FastQCFastQC Report
Sat 18 Jun 2016
SRR1930973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794755
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.2659973088248814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42930.2391969934615031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.1482653621246354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.058.2734531
ACGACCG502.84791E-547.442615
GTATCAA47500.040.8246351
ATCAACG56650.033.9752273
CAACGCA57200.033.757245
TCAACGC57650.033.467394
TATCAAC59500.032.269772
AACGCAG60500.031.915946
CGACCGA804.526568E-429.651636
TATCACG856.3880946E-427.9564862
ACGCAGA70050.027.4293487
CGCAGAG71450.026.8254938
GGCGTGC5750.025.5777538
ACGGGCG1601.8801929E-623.7213045
GCAGAGT80750.023.2659769
GCGTGCG6100.022.5546829
ACGTGCG1703.18385E-622.3259359
GAGTACT57650.021.7256712-13
CCGATCG1353.8282148E-421.0856049
GGCGTCG1805.2258474E-621.0856048
Produced by FastQC (version 0.11.5)