##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930973_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1794755 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.275492198099464 31.0 31.0 34.0 30.0 34.0 2 31.44109641705971 31.0 31.0 34.0 30.0 34.0 3 31.505300166317966 31.0 31.0 34.0 30.0 34.0 4 35.28133589264273 37.0 35.0 37.0 33.0 37.0 5 35.15778086702642 37.0 35.0 37.0 32.0 37.0 6 35.187318603374834 37.0 35.0 37.0 32.0 37.0 7 35.06682416262944 37.0 35.0 37.0 32.0 37.0 8 35.068230482712124 37.0 35.0 37.0 32.0 37.0 9 36.56307629732192 39.0 35.0 39.0 32.0 39.0 10-11 36.498346292390885 38.0 35.0 39.0 32.0 39.0 12-13 36.38023212081872 38.0 35.0 39.0 32.0 39.0 14-15 37.423484263868886 39.0 36.0 41.0 32.0 41.0 16-17 37.37423297330277 39.0 36.0 41.0 32.0 41.0 18-19 37.40484244367616 39.0 36.0 41.0 32.0 41.0 20-21 37.3913163635148 39.0 36.0 41.0 32.0 41.0 22-23 37.28703583497469 39.0 36.0 40.0 31.0 41.0 24-25 37.15674757836028 39.0 36.0 40.0 31.0 41.0 26-27 36.89033739981223 39.0 36.0 40.0 31.0 41.0 28-29 36.69908845497018 39.0 35.0 40.0 30.0 41.0 30-31 36.470800471373536 38.0 35.0 40.0 30.0 41.0 32-33 36.29788021206237 38.0 35.0 40.0 30.0 41.0 34-35 36.37850430838749 38.0 35.0 40.0 30.0 41.0 36-37 36.44055372460308 38.0 35.0 40.0 30.0 41.0 38-39 36.42123743909336 38.5 35.0 40.0 30.0 41.0 40-41 36.307746461216155 38.0 35.0 40.0 30.0 41.0 42-43 36.151784226816474 38.0 35.0 40.0 30.0 41.0 44-45 35.96159197216333 38.0 35.0 40.0 29.0 41.0 46-47 35.71014428153146 38.0 35.0 40.0 28.5 41.0 48-49 35.47396747745514 38.0 34.0 40.0 27.0 41.0 50-51 35.2081749319545 38.0 34.0 40.0 26.5 41.0 52-53 35.0055249880903 38.0 34.0 40.0 26.0 41.0 54-55 34.84326272945333 38.0 34.0 40.0 26.0 41.0 56-57 34.66999284024839 38.0 33.0 40.0 26.0 41.0 58-59 34.457431515722206 38.0 33.0 40.0 25.5 41.0 60-61 34.147861407267285 37.0 33.0 40.0 24.5 41.0 62-63 33.89937985964658 37.0 32.5 40.0 24.0 41.0 64-65 33.583682731069146 36.5 32.0 39.5 23.0 41.0 66-67 33.25675092143496 36.0 32.0 39.0 22.5 40.5 68-69 32.89958044412747 36.0 31.0 39.0 22.0 40.0 70-71 32.539079428668536 35.0 31.0 38.5 22.0 40.0 72-73 32.105609400726 35.0 31.0 38.0 20.5 40.0 74-75 31.589101855127858 35.0 30.0 37.0 20.0 39.0 76-77 30.23133937501219 33.5 28.5 36.0 18.5 38.5 78-79 30.95902170491237 34.0 30.0 36.0 19.5 39.0 80-81 30.92158734757669 34.0 30.0 36.0 19.5 38.5 82-83 30.646911695468184 34.0 30.0 36.0 18.5 37.0 84-85 30.299690208412848 34.0 30.0 35.0 18.0 37.0 86-87 29.944548698847477 34.0 29.5 35.0 15.0 36.5 88-89 29.593102122573832 34.0 29.0 35.0 9.5 36.0 90-91 29.243073567144265 34.0 29.0 35.0 7.0 36.0 92-93 28.90213622472148 34.0 29.0 35.0 2.0 35.0 94-95 28.501847327351086 34.0 29.0 35.0 2.0 35.0 96-97 28.064166418257646 33.0 29.0 35.0 2.0 35.0 98-99 27.532388543283066 33.0 27.0 35.0 2.0 35.0 100-101 26.366426615331896 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3761705667031663 1101 2 0.39016297729425276 1101 3 0.30289969261989214 1101 4 0.18834752762460027 1101 5 0.35088454456127494 1101 6 0.38094541474251997 1101 7 0.4046683236125048 1101 8 0.447436252461749 1101 9 0.6518611085905874 1101 10-11 0.5216922085703004 1101 12-13 0.4745243704591715 1101 14-15 0.4593827831288664 1101 16-17 0.5913778727736414 1101 18-19 0.5231654807271866 1101 20-21 0.47883260546753803 1101 22-23 0.5060721948286755 1101 24-25 0.3959939987643537 1101 26-27 0.4787124250646855 1101 28-29 0.6069480081817389 1101 30-31 0.5645397959202683 1101 32-33 0.5076777168232596 1101 34-35 0.5490745694237305 1101 36-37 0.41343933082921325 1101 38-39 0.4757788196335184 1101 40-41 0.5589857013424862 1101 42-43 0.610143491242944 1101 44-45 0.6123034616833607 1101 46-47 0.519242008465227 1101 48-49 0.4673684109141618 1101 50-51 0.5043034331747336 1101 52-53 0.42910593230971017 1101 54-55 0.5453977516606088 1101 56-57 0.5795659916722187 1101 58-59 0.5913472054188418 1101 60-61 0.3644366686487359 1101 62-63 0.3167089799219269 1101 64-65 0.5169653843792119 1101 66-67 0.5384585942549265 1101 68-69 0.35313927537615797 1101 70-71 0.23153228192530406 1101 72-73 0.4321570176917007 1101 74-75 0.5177237210089523 1101 76-77 0.32603093792573645 1101 78-79 0.45491082822668005 1101 80-81 0.5637727862159849 1101 82-83 0.5475052244971721 1101 84-85 0.6226679959922841 1101 86-87 0.6349108297245856 1101 88-89 0.5332219475670499 1101 90-91 0.46383891235730346 1101 92-93 0.45151033744384605 1101 94-95 0.5921954752111454 1101 96-97 0.7666499457325671 1101 98-99 0.896724661733149 1101 100-101 1.3725619457532723 1102 1 0.23864602312367467 1102 2 0.3545263753866088 1102 3 0.4185117734650916 1102 4 0.13059355786949567 1102 5 0.1960652213335905 1102 6 0.28083575673140615 1102 7 0.3196462118739234 1102 8 0.32250042604114526 1102 9 0.4660954840127829 1102 10-11 0.28117329200046015 1102 12-13 0.4347294331916558 1102 14-15 0.40208056159716676 1102 16-17 0.5680246005966012 1102 18-19 0.5237509239076772 1102 20-21 0.3824391043371804 1102 22-23 0.47721237919824944 1102 24-25 0.5390442252858278 1102 26-27 0.4328288787201018 1102 28-29 0.38662294085578 1102 30-31 0.33882780694461445 1102 32-33 0.4074819311134803 1102 34-35 0.4752566580147999 1102 36-37 0.6278086396459912 1102 38-39 0.46515077420312423 1102 40-41 0.5262394436754292 1102 42-43 0.5160233673091241 1102 44-45 0.7968689277411656 1102 46-47 0.6997949370321521 1102 48-49 0.5954735114752836 1102 50-51 0.46188751182226895 1102 52-53 0.4803901919942888 1102 54-55 0.6266761231378055 1102 56-57 0.635462305181548 1102 58-59 0.6941403323159108 1102 60-61 0.59707743395807 1102 62-63 0.24587288825053122 1102 64-65 0.23622258082691872 1102 66-67 0.40301378128150134 1102 68-69 0.48298747671457676 1102 70-71 0.6267523072344616 1102 72-73 0.42650326905398117 1102 74-75 0.4113340590348322 1102 76-77 0.5130448512080896 1102 78-79 0.6976710352033599 1102 80-81 0.5910071526313629 1102 82-83 0.4970763310748545 1102 84-85 0.5077767420630614 1102 86-87 0.5696586838501609 1102 88-89 0.602114536757064 1102 90-91 0.6288424137578623 1102 92-93 0.6050247978904011 1102 94-95 0.5995453202938741 1102 96-97 0.5841304347127227 1102 98-99 0.6970156382523776 1102 100-101 0.943649665407051 1103 1 0.25177627522451473 1103 2 0.40652217370593746 1103 3 0.39855379027181215 1103 4 0.2939338940039491 1103 5 0.3520526162915729 1103 6 0.386928193706197 1103 7 0.4640789849831606 1103 8 0.332479417637785 1103 9 0.4808013663657249 1103 10-11 0.34997581300886793 1103 12-13 0.39402565167905124 1103 14-15 0.52392930109297 1103 16-17 0.5459657770671953 1103 18-19 0.6062089071009495 1103 20-21 0.5147920455136585 1103 22-23 0.3989560766772371 1103 24-25 0.5542753177227979 1103 26-27 0.558354088804137 1103 28-29 0.6119380669061982 1103 30-31 0.5518004960202418 1103 32-33 0.6312214269117931 1103 34-35 0.6677461538131197 1103 36-37 0.6664115808224622 1103 38-39 0.669982706976235 1103 40-41 0.640997847036779 1103 42-43 0.5619792496620661 1103 44-45 0.5053773311025154 1103 46-47 0.5682298109817339 1103 48-49 0.44316258710554024 1103 50-51 0.5627278479567224 1103 52-53 0.5982998558598425 1103 54-55 0.62089881221344 1103 56-57 0.6934980194672633 1103 58-59 0.6965037776940619 1103 60-61 0.4497560053866394 1103 62-63 0.3811144848891814 1103 64-65 0.272199471583221 1103 66-67 0.3250200838025137 1103 68-69 0.2920736111683553 1103 70-71 0.24728802152017693 1103 72-73 0.2607994875413766 1103 74-75 0.5415861598751697 1103 76-77 0.6004923301996854 1103 78-79 0.6724609511697466 1103 80-81 0.4998832030515281 1103 82-83 0.5703431377975399 1103 84-85 0.5757914479454129 1103 86-87 0.5704464989762066 1103 88-89 0.7108330241520235 1103 90-91 0.5910272877074441 1103 92-93 0.37472017604166297 1103 94-95 0.4165096060081588 1103 96-97 0.6298237120236472 1103 98-99 0.6374042937145603 1103 100-101 1.1009500855751178 1104 1 0.2939383238865503 1104 2 0.38613744032009123 1104 3 0.312168834943229 1104 4 0.17021360060435597 1104 5 0.3171627281768252 1104 6 0.2746523667519014 1104 7 0.2757831461919267 1104 8 0.255674990532917 1104 9 0.408377316476475 1104 10-11 0.3250239821988572 1104 12-13 0.4245567856758967 1104 14-15 0.505333573055502 1104 16-17 0.5400245929809273 1104 18-19 0.4626698886971141 1104 20-21 0.29509688495330977 1104 22-23 0.37652122883856975 1104 24-25 0.33166131096007234 1104 26-27 0.458365077145622 1104 28-29 0.48079397928668044 1104 30-31 0.424155542388867 1104 32-33 0.40647258123162544 1104 34-35 0.38426761480559435 1104 36-37 0.5045271999339036 1104 38-39 0.5425859429883957 1104 40-41 0.44462340134965217 1104 42-43 0.5054076502817679 1104 44-45 0.5029971584223532 1104 46-47 0.48813228129145614 1104 48-49 0.48367148875009036 1104 50-51 0.42485326518730915 1104 52-53 0.42560270216667107 1104 54-55 0.5751684084517308 1104 56-57 0.7128311087361112 1104 58-59 0.6721095236174932 1104 60-61 0.6172933983863089 1104 62-63 0.5421828998263649 1104 64-65 0.41821331471757617 1104 66-67 0.29199984567100756 1104 68-69 0.3356410251873072 1104 70-71 0.4099908162579524 1104 72-73 0.4163865152058861 1104 74-75 0.5151918372574649 1104 76-77 0.4353326619823221 1104 78-79 0.6507733921482135 1104 80-81 0.508007096057721 1104 82-83 0.5577865097605148 1104 84-85 0.45068945220149104 1104 86-87 0.6155779837296116 1104 88-89 0.6567078266882618 1104 90-91 0.48800601057929427 1104 92-93 0.4331867203496991 1104 94-95 0.6737950939993169 1104 96-97 0.5779678518649796 1104 98-99 0.675847985536187 1104 100-101 0.824724939898001 1105 1 0.31850639687606375 1105 2 0.3327785065639901 1105 3 0.38882507929283605 1105 4 0.16071065886151814 1105 5 0.26575320483024 1105 6 0.32391056788330275 1105 7 0.37453350975336264 1105 8 0.44007922651440623 1105 9 0.4384584364139599 1105 10-11 0.4709646868977728 1105 12-13 0.4837711617964544 1105 14-15 0.4549305373275345 1105 16-17 0.6142611006117704 1105 18-19 0.6015275219114926 1105 20-21 0.5922738053656076 1105 22-23 0.37706126279501007 1105 24-25 0.35151009611068673 1105 26-27 0.4765009504368791 1105 28-29 0.4376811348915126 1105 30-31 0.4567365227775184 1105 32-33 0.5792635498981724 1105 34-35 0.5411269140594541 1105 36-37 0.4246784254664533 1105 38-39 0.4185909950568032 1105 40-41 0.3526195895966211 1105 42-43 0.5649228910175239 1105 44-45 0.425346311474442 1105 46-47 0.4486876349055322 1105 48-49 0.42873732241482543 1105 50-51 0.46049390383755906 1105 52-53 0.3130681187606683 1105 54-55 0.4268279979671661 1105 56-57 0.45993216532777126 1105 58-59 0.4407937770873218 1105 60-61 0.43321473183750925 1105 62-63 0.2601531039469194 1105 64-65 0.5366512811879289 1105 66-67 0.6710045967412626 1105 68-69 0.3531781071183602 1105 70-71 0.39589972251826566 1105 72-73 0.5180216002131601 1105 74-75 0.6724791307819409 1105 76-77 0.7446805108871217 1105 78-79 0.8203559394899855 1105 80-81 0.6633610039191709 1105 82-83 0.7377998316664254 1105 84-85 0.8179739707740374 1105 86-87 0.8779209779374675 1105 88-89 0.9090059756430904 1105 90-91 0.84307219411766 1105 92-93 0.705015696777032 1105 94-95 0.9532818961516547 1105 96-97 0.8088549364267656 1105 98-99 0.8727913185605978 1105 100-101 0.9827872969939833 1106 1 0.2753782614735627 1106 2 0.22709704001609765 1106 3 0.3655572280105446 1106 4 0.17778225152878235 1106 5 0.15132496159333186 1106 6 0.33471276207900047 1106 7 0.2939090079014193 1106 8 0.1838984397921024 1106 9 0.24097707301201865 1106 10-11 0.16626591996073614 1106 12-13 0.28410873036506956 1106 14-15 0.4294497365398726 1106 16-17 0.3742020261122647 1106 18-19 0.4266004273454129 1106 20-21 0.33637245040288377 1106 22-23 0.34936001097823066 1106 24-25 0.37199113132096784 1106 26-27 0.31200191156960955 1106 28-29 0.6035585787472897 1106 30-31 0.6198018329186397 1106 32-33 0.6582095858117185 1106 34-35 0.7436896221095282 1106 36-37 0.5975116572166499 1106 38-39 0.6350896588478889 1106 40-41 0.5196475796570965 1106 42-43 0.6411608768584003 1106 44-45 0.6895809216296342 1106 46-47 0.5367620875287216 1106 48-49 0.6388081180451834 1106 50-51 0.5436426684303655 1106 52-53 0.535682399786495 1106 54-55 0.7135315471255907 1106 56-57 0.8983652845551191 1106 58-59 0.9070528609637307 1106 60-61 0.8705123079076529 1106 62-63 0.5086851495186693 1106 64-65 0.48568686654120796 1106 66-67 0.5302244382715742 1106 68-69 0.4733665829025071 1106 70-71 0.6118048282428603 1106 72-73 0.7181772568309128 1106 74-75 0.8727970078507248 1106 76-77 0.7682578000239815 1106 78-79 0.7240251222927405 1106 80-81 0.7409689555343419 1106 82-83 0.7721193028089992 1106 84-85 0.84620970615779 1106 86-87 0.8068702957676521 1106 88-89 0.7961080432505732 1106 90-91 0.8579514664698564 1106 92-93 0.8136115053006385 1106 94-95 0.9651230513863105 1106 96-97 0.865296401099279 1106 98-99 1.0196636520033273 1106 100-101 1.302004325452888 1107 1 0.23064690111948138 1107 2 0.30051704367059884 1107 3 0.22699182259782447 1107 4 0.06919187369400248 1107 5 0.1376549630145263 1107 6 0.2669569639427962 1107 7 0.1766609823469878 1107 8 0.2534135416782277 1107 9 0.1897785997812349 1107 10-11 0.31214303398792254 1107 12-13 0.2420528706437608 1107 14-15 0.34136652220339414 1107 16-17 0.24877035512561463 1107 18-19 0.3011965034943813 1107 20-21 0.36331518978960275 1107 22-23 0.2555880924329941 1107 24-25 0.26301608344024174 1107 26-27 0.3445046355262136 1107 28-29 0.3977526598095338 1107 30-31 0.26013168545276244 1107 32-33 0.2507599536304639 1107 34-35 0.4112476711475139 1107 36-37 0.241364177807057 1107 38-39 0.29982372623959463 1107 40-41 0.1274493974328621 1107 42-43 0.2926144883849702 1107 44-45 0.3218337860138192 1107 46-47 0.4400472130337505 1107 48-49 0.2188335641988246 1107 50-51 0.3338366953518559 1107 52-53 0.21706014658373363 1107 54-55 0.39522994252903487 1107 56-57 0.2382644474037292 1107 58-59 0.3758780743121406 1107 60-61 0.1904527530799882 1107 62-63 0.1055146670568945 1107 64-65 0.07286322327462358 1107 66-67 0.1618094764746374 1107 68-69 0.2622920919947731 1107 70-71 0.2309829716211098 1107 72-73 0.1278999885025769 1107 74-75 0.28474884965144653 1107 76-77 0.21399956217011962 1107 78-79 0.30346706648716193 1107 80-81 0.41103114972368715 1107 82-83 0.5190586583986949 1107 84-85 0.4328431664963013 1107 86-87 0.36491378432703314 1107 88-89 0.5106373909221844 1107 90-91 0.5295849606463428 1107 92-93 0.4950082013612196 1107 94-95 0.8802112669747864 1107 96-97 0.625239824012997 1107 98-99 0.6850664930393116 1107 100-101 0.8638211537522587 1108 1 0.10765243324074802 1108 2 0.2502782871187108 1108 3 0.1240721533335396 1108 4 0.14545594358943248 1108 5 0.21270294212288832 1108 6 0.10658610342984076 1108 7 0.14871084341717733 1108 8 0.1767251474868914 1108 9 0.2247672921048789 1108 10-11 0.18838133170883964 1108 12-13 0.24217404838900336 1108 14-15 0.27185821269899435 1108 16-17 0.23130040626289627 1108 18-19 0.3063384027688514 1108 20-21 0.22043651877113746 1108 22-23 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-1.0276487116574238 2316 90-91 -1.0026772652198233 2316 92-93 -0.7854912279330222 2316 94-95 -1.3157454856447544 2316 96-97 -1.0037114458318186 2316 98-99 -1.1230252166900563 2316 100-101 -1.624834133304308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 24.0 5 48.0 6 102.0 7 255.0 8 623.0 9 1235.0 10 2116.0 11 3485.0 12 4948.0 13 6375.0 14 8128.0 15 10265.0 16 12454.0 17 14467.0 18 12469.0 19 9542.0 20 9251.0 21 9706.0 22 10955.0 23 12483.0 24 15460.0 25 19634.0 26 25341.0 27 32769.0 28 40839.0 29 51683.0 30 62519.0 31 75350.0 32 90689.0 33 109615.0 34 134757.0 35 167798.0 36 218948.0 37 296906.0 38 274417.0 39 49070.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.88610687788528 22.116829603296438 15.208743693112641 22.78831982570563 2 15.185137099690579 21.94659327788312 40.58818383377641 22.280085788649888 3 18.66353823763431 26.17967999536419 31.005714527693396 24.151067239308105 4 12.463743688457754 18.195380288733347 39.30343232927233 30.03744369353657 5 11.983779139545534 38.813947087187685 36.37301738538362 12.829256387883165 6 29.34348370187202 40.029731226591394 17.50743559255739 13.119349478979197 7 25.75504734629518 33.435092812110845 22.884014809820837 17.92584503177314 8 24.11927501597722 37.96671724029569 20.298839443864665 17.615168299862432 9 24.5529891266496 16.781900593674344 21.451451590885664 37.21365868879039 10-11 23.611607155294177 27.92865878629674 29.015826672721346 19.44390738568774 12-13 24.135550534752653 25.105905820014428 29.594707912779185 21.163835732453734 14-15 21.23378957016417 27.048120774144664 27.19527177804213 24.52281787764904 16-17 20.443486715456984 30.168964566194273 28.091717253887023 21.295831464461724 18-19 20.277920941855573 28.932556254196257 30.51915163908166 20.270371164866514 20-21 20.97879654883257 28.200785065371036 30.596822407515234 20.223595978281157 22-23 20.637794097613117 27.852844005720023 30.53023863789272 20.97912325877414 24-25 20.57728995937034 28.12832219342214 30.72060812994359 20.57377971726393 26-27 20.488959082815864 28.299410963495227 30.576017691989875 20.63561226169903 28-29 20.4052312490911 28.311360950504056 30.70985255352686 20.573555246877984 30-31 20.813659072597048 28.167337736801574 30.5588678770075 20.460135313593877 32-33 20.683408684021707 28.357949439572 30.3503005900342 20.608341286372088 34-35 20.64864897998874 28.324513399027985 30.63165552577034 20.395182095212938 36-37 20.822000469705003 28.119422078976747 30.573978955767217 20.484598495551033 38-39 20.572613772651106 28.447292871858377 30.59655857841123 20.38353477707929 40-41 20.666718299288306 28.41016286006092 30.487047919716105 20.436070920934668 42-43 20.753125158466 28.315303733695764 30.28630034621512 20.645270761623117 44-45 20.62021313644912 28.663063314062825 30.109716514592183 20.60700703489587 46-47 20.802973194663004 28.544362765857855 29.8087339125628 20.843930126916337 48-49 20.872818010696665 28.644004328594242 29.743510730656453 20.739666930052636 50-51 20.863244697730668 28.8442726057628 29.47983880308584 20.812643893420695 52-53 20.88079365473364 28.970037698655627 29.32164452820064 20.82752411841009 54-55 20.96138226390017 28.79900359302794 29.399606285500134 20.84000785757176 56-57 20.893318485461336 29.02453437182998 29.303948885727017 20.77819825698167 58-59 20.782693256132116 28.99425727731973 29.52247073607717 20.70057873047098 60-61 20.900725757415163 29.05986243522015 29.41083113449105 20.628580672873632 62-63 20.81413684421866 28.99903858155735 29.392988375018536 20.793836199205455 64-65 20.648333828083352 28.784481586223475 29.567238482261466 20.999946103431704 66-67 20.721045045276526 28.95284611064269 29.298323471262677 21.02778537281811 68-69 20.718847065355266 29.00419637914981 29.314749661100354 20.96220689439457 70-71 20.79893445621246 28.594185861440213 29.504700846347802 21.102178835999517 72-73 20.918469418533174 28.764405668766507 29.30548932607015 21.011635586630167 74-75 20.680321094216332 29.1441965982828 29.118247841630463 21.057234465870405 76-77 20.664925653333633 29.032230148749168 29.241514653683975 21.06132954423323 78-79 20.72319729329668 29.1021591609086 29.105753165440383 21.068890380354336 80-81 20.71664954733363 29.10013597960055 29.03213224984253 21.151082223223288 82-83 20.81757677231711 28.922137004772242 29.24184638014659 21.018439842764053 84-85 20.946034416953847 28.903722235068297 29.046192934411664 21.104050413566195 86-87 20.61537647199757 29.084025396223996 29.097091246437536 21.203506885340893 88-89 20.76695811818064 29.07693400622091 29.03798713193045 21.118120743667998 90-91 20.78855333355436 28.98044107121514 29.259575734257336 20.971429860973164 92-93 20.791295162502394 28.99547800287609 29.08664797385049 21.126578860771026 94-95 20.519259668280483 28.971409849730513 29.1479844364741 21.3613460455149 96-97 20.59072129622149 28.87502751071873 29.2305913620522 21.303659831007575 98-99 20.501433343269696 28.940997517767048 29.117010399748157 21.440558739215103 100-101 20.51536282110929 28.914949394207007 29.027248844549813 21.54243894013389 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 80.0 2 73.5 3 105.5 4 106.5 5 112.5 6 219.5 7 503.5 8 827.0 9 1059.5 10 1027.5 11 922.0 12 1022.0 13 1177.0 14 1344.5 15 1533.0 16 1804.5 17 2061.0 18 2295.5 19 2651.5 20 3089.5 21 3698.0 22 4599.5 23 5896.5 24 7545.0 25 9586.5 26 12843.0 27 16890.5 28 20333.5 29 24191.0 30 28853.5 31 33295.0 32 39093.5 33 45929.5 34 51854.5 35 56765.5 36 63226.5 37 69507.0 38 71143.0 39 71099.0 40 71609.0 41 71453.0 42 71094.5 43 72354.5 44 73948.0 45 75432.0 46 74620.0 47 72168.0 48 71065.5 49 69355.5 50 65194.5 51 59534.5 52 53451.0 53 47489.5 54 42393.0 55 35692.0 56 28273.0 57 21974.0 58 17186.0 59 13540.0 60 10094.5 61 7089.0 62 4660.0 63 2798.5 64 1629.0 65 984.5 66 517.5 67 262.5 68 156.5 69 91.5 70 56.0 71 39.5 72 30.0 73 27.0 74 23.0 75 14.5 76 10.5 77 7.0 78 4.0 79 4.0 80 2.0 81 1.0 82 0.5 83 0.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005014612022253734 2 0.006240406072137979 3 0.0017272552521096194 4 0.1670422982524077 5 0.0016158194293928697 6 5.014612022253734E-4 7 0.0 8 6.686149363004978E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.6216642382943636E-4 24-25 3.900253795086237E-4 26-27 0.0024794470554476795 28-29 1.1143582271674963E-4 30-31 0.006073252338062855 32-33 0.020476332424202746 34-35 0.013400157681689144 36-37 6.407559806213104E-4 38-39 1.3929477839593705E-4 40-41 0.0013372298726009957 42-43 0.011477889739825213 44-45 0.007243328476588726 46-47 0.010419249424016092 48-49 0.009862070310432344 50-51 0.18283832612250697 52-53 0.1106279130020532 54-55 0.017133257742700258 56-57 0.006101111293742042 58-59 0.017272552521096194 60-61 0.11951491986371399 62-63 0.23242726723146057 64-65 0.23894626286039045 66-67 0.24953266601848165 68-69 0.24527024579956594 70-71 0.22955779479650423 72-73 0.1557594212023368 74-75 0.26123342740374034 76-77 0.20350967123646405 78-79 0.005543932180158295 80-81 2.7858955679187408E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.3929477839593705E-4 90-91 0.005655368002875045 92-93 0.016966104008625135 94-95 0.0025630239224852416 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1794755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.929769600332236 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9927418172066 44.78234766585519 2 14.415301662620022 16.98980811394968 3 4.485467852957522 7.929827613734511 4 1.8374941632401074 4.331324307267793 5 0.9100466839667571 2.6814420705853683 6 0.5339514461334418 1.8879381419044765 7 0.3558697771032544 1.4679926780691457 8 0.24012874968722284 1.1320585514787116 9 0.17927413547375903 0.9508125148890632 >10 0.941065187841612 10.174032641822432 >50 0.07160654162567062 2.9121197284853033 >100 0.035623582280795346 3.5961519209476345 >500 9.522665754956479E-4 0.37547784262774614 >1k 4.7613328774782394E-4 0.7886662083829538 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4774 0.2659973088248814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4293 0.2391969934615031 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2661 0.1482653621246354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.571791135837482E-5 0.0 5 0.0 0.0 0.0 5.571791135837482E-5 0.0 6 0.0 0.0 0.0 5.571791135837482E-5 0.0 7 0.0 0.0 0.0 1.1143582271674965E-4 0.0 8 0.0 0.0 0.0 1.1143582271674965E-4 0.0 9 0.0 0.0 0.0 1.1143582271674965E-4 0.0 10-11 0.0 0.0 0.0 1.1143582271674965E-4 0.0 12-13 0.0 0.0 0.0 1.1143582271674965E-4 0.0 14-15 0.0 0.0 0.0 1.3929477839593705E-4 0.0 16-17 0.0 0.0 0.0 1.6715373407512446E-4 0.0 18-19 0.0 0.0 0.0 1.6715373407512446E-4 0.0 20-21 0.0 0.0 0.0 1.9501268975431188E-4 0.0 22-23 0.0 0.0 0.0 2.507306011126867E-4 0.0 24-25 0.0 0.0 0.0 2.785895567918741E-4 0.0 26-27 0.0 0.0 0.0 3.0644851247106154E-4 0.0 28-29 0.0 0.0 0.0 6.128970249421231E-4 0.0 30-31 0.0 0.0 0.0 0.0025351649668060544 0.0 32-33 0.0 0.0 0.0 0.007048315786834415 0.0 34-35 0.0 0.0 0.0 0.013818042016876957 0.0 36-37 0.0 0.0 0.0 0.025992405648681854 0.0 38-39 0.0 0.0 0.0 0.044602188042379046 0.0 40-41 0.0 0.0 0.0 0.0655242637574488 0.0 42-43 0.0 0.0 0.0 0.08683636485202716 0.0 44-45 0.0 0.0 0.0 0.11463960261985619 0.0 46-47 0.0 0.0 0.0 0.1471231449417887 0.0 48-49 0.0 0.0 0.0 0.18175182685101865 0.0 50-51 0.0 0.0 0.0 0.21975144239743027 0.0 52-53 0.0 0.0 0.0 0.2573610325643333 0.0 54-55 0.0 0.0 0.0 0.29987379893077326 0.0 56-57 0.0 0.0 0.0 0.35341871174617145 0.0 58-59 0.0 0.0 0.0 0.41236826196333204 0.0 60-61 0.0 0.0 0.0 0.4763602831584255 0.0 62-63 0.0 0.0 0.0 0.5408537655557444 0.0 64-65 0.0 0.0 0.0 0.6071302211165313 0.0 66-67 0.0 0.0 0.0 0.6850517201512184 0.0 68-69 0.0 0.0 0.0 0.7786856701889673 0.0 70-71 0.0 0.0 0.0 0.8825995748723363 0.0 72-73 0.0 0.0 0.0 0.9897729773701703 0.0 74-75 0.0 0.0 0.0 1.102267440402729 0.0 76-77 0.0 0.0 0.0 1.2250975759922662 0.0 78-79 0.0 0.0 0.0 1.3660081738175962 0.0 80-81 0.0 0.0 0.0 1.5284816033386173 0.0 82-83 0.0 0.0 0.0 1.703407986048235 0.0 84-85 0.0 0.0 0.0 1.8746012686968416 0.0 86-87 0.0 0.0 0.0 2.0542636738719215 0.0 88-89 0.0 0.0 0.0 2.2623143548840927 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2455 0.0 58.273453 1 ACGACCG 50 2.84791E-5 47.44261 5 GTATCAA 4750 0.0 40.824635 1 ATCAACG 5665 0.0 33.975227 3 CAACGCA 5720 0.0 33.75724 5 TCAACGC 5765 0.0 33.46739 4 TATCAAC 5950 0.0 32.26977 2 AACGCAG 6050 0.0 31.91594 6 CGACCGA 80 4.526568E-4 29.65163 6 TATCACG 85 6.3880946E-4 27.956486 2 ACGCAGA 7005 0.0 27.429348 7 CGCAGAG 7145 0.0 26.825493 8 GGCGTGC 575 0.0 25.577753 8 ACGGGCG 160 1.8801929E-6 23.721304 5 GCAGAGT 8075 0.0 23.265976 9 GCGTGCG 610 0.0 22.554682 9 ACGTGCG 170 3.18385E-6 22.325935 9 GAGTACT 5765 0.0 21.72567 12-13 CCGATCG 135 3.8282148E-4 21.085604 9 GGCGTCG 180 5.2258474E-6 21.085604 8 >>END_MODULE