FastQCFastQC Report
Sat 18 Jun 2016
SRR1930972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163900
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74514.546064673581452No Hit
NNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6190.3776693105552166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT5180.31604636973764494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA5140.3136058572300183No Hit
NNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4460.27211714460036607No Hit
NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4230.25808419768151314No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNN4200.25625381330079317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNG4040.24649176327028677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNN3510.2141549725442343No Hit
NNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3460.21110433190970104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.2050030506406345No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNN2870.17510677242220865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.1683953630262355No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNN2310.14093959731543623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.13971934106162293No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11775472849298353No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNANNNNNNNNNNNNNNN1640.10006101281269067No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGT255.676085E-574.8278666
ATACGGG309.477796E-567.4653553
CTAAGTC250.00364585961.7597162
TACGGGC352.0317902E-457.8274424
ACGGGCG352.0365002E-457.8003855
GTATACG459.368781E-657.1849251
GGCGTCG250.006681144753.00188
GGTATAT250.006681144753.00188
CCTCTCG250.00706734752.2543959
GGTATCA1900.048.7576681
GTATCAA4050.048.289491
GCTAAGT456.45766E-445.747941
CGATCTC207.8467093E-443.99735392-93
TATACGG605.134718E-542.888692
GTATTAC500.001084832541.1731451
TATCAAC4850.040.324212
ATCAACG4650.040.2615853
CAACGCA5000.036.4142465
TCAACGC5100.035.7169534
GACCGTC300.003190813133.06700522-23