##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930972_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 163900 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.620695546064674 31.0 2.0 31.0 2.0 33.0 2 21.126772422208663 30.0 2.0 31.0 2.0 34.0 3 21.97327028676022 30.0 2.0 31.0 2.0 34.0 4 23.36939597315436 33.0 2.0 37.0 2.0 37.0 5 22.61679072605247 33.0 2.0 37.0 2.0 37.0 6 23.86688224527151 33.0 2.0 37.0 2.0 37.0 7 24.276168395363026 33.0 2.0 37.0 2.0 37.0 8 24.898669920683343 33.0 10.0 37.0 2.0 37.0 9 26.580726052471018 35.0 15.0 39.0 2.0 39.0 10-11 27.221659548505187 35.0 13.0 39.0 2.0 39.0 12-13 27.260878584502745 35.0 12.5 39.0 2.0 39.0 14-15 27.336799877974375 36.0 5.0 40.0 2.0 41.0 16-17 25.959426479560708 35.5 2.0 39.0 2.0 41.0 18-19 24.635320317266626 34.0 2.0 39.0 2.0 40.5 20-21 24.26888346552776 33.5 2.0 39.0 2.0 40.0 22-23 23.894752898108603 33.0 2.0 39.0 2.0 40.0 24-25 23.799792556436852 33.0 2.0 39.0 2.0 40.0 26-27 23.529978645515556 32.5 2.0 38.5 2.0 40.0 28-29 23.425247101891397 32.0 2.0 38.0 2.0 40.0 30-31 23.586812080536916 32.0 2.0 38.0 2.0 40.0 32-33 23.49275777913362 32.5 2.0 38.0 2.0 40.0 34-35 23.211073825503355 31.0 2.0 38.0 2.0 40.0 36-37 22.68473154362416 30.0 2.0 38.0 2.0 40.0 38-39 22.673602806589386 30.0 2.0 38.0 2.0 40.0 40-41 22.6690634533252 30.5 2.0 38.0 2.0 40.0 42-43 22.595036607687614 30.5 2.0 38.0 2.0 40.0 44-45 22.24606467358145 30.0 2.0 38.0 2.0 40.0 46-47 21.88956375838926 30.0 2.0 38.0 2.0 40.0 48-49 21.755683343502135 29.5 2.0 38.0 2.0 40.0 50-51 18.91467053081147 16.0 2.0 35.5 2.0 39.0 52-53 21.15154362416107 28.0 2.0 36.5 2.0 39.0 54-55 22.13318791946309 30.5 2.0 38.0 2.0 40.0 56-57 22.1937278828554 31.0 2.0 38.0 2.0 40.0 58-59 21.951995118974985 30.5 2.0 38.0 2.0 40.0 60-61 21.725195241000613 30.0 2.0 38.0 2.0 40.0 62-63 21.339624771201954 28.5 2.0 37.0 2.0 40.0 64-65 21.060347773032337 28.0 2.0 36.5 2.0 39.5 66-67 20.80049115314216 28.0 2.0 36.0 2.0 39.0 68-69 20.39954850518609 27.5 2.0 35.5 2.0 39.0 70-71 20.126644295302015 26.0 2.0 35.0 2.0 39.0 72-73 19.791934106162294 26.0 2.0 35.0 2.0 38.0 74-75 19.39526235509457 26.0 2.0 35.0 2.0 37.5 76-77 19.081342281879195 25.5 2.0 34.0 2.0 37.0 78-79 18.79323672971324 25.0 2.0 34.0 2.0 36.0 80-81 18.448441122635753 24.0 2.0 34.0 2.0 36.0 82-83 18.064929835265403 22.5 2.0 34.0 2.0 35.0 84-85 17.76647345942648 20.5 2.0 34.0 2.0 35.0 86-87 17.379237339841367 19.0 2.0 33.5 2.0 35.0 88-89 17.049392922513725 18.0 2.0 33.0 2.0 35.0 90-91 16.633212324588165 11.5 2.0 33.0 2.0 35.0 92-93 16.161275167785234 4.5 2.0 32.0 2.0 35.0 94-95 15.656223306894447 2.0 2.0 32.0 2.0 35.0 96-97 15.078880414887127 2.0 2.0 32.0 2.0 34.0 98-99 14.421726662599145 2.0 2.0 31.5 2.0 34.0 100-101 13.235042708968884 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.720893957709741 1101 2 10.424465960205087 1101 3 9.9538262032192 1101 4 11.941505372873891 1101 5 12.659077418470822 1101 6 11.63761892550659 1101 7 11.228690263898496 1101 8 10.381357052571339 1101 9 10.14178599621619 1101 10-11 9.430028461565833 1101 12-13 9.879476128525894 1101 14-15 10.8019014436552 1101 16-17 12.07884090532404 1101 18-19 13.301715541199474 1101 20-21 13.563550359740859 1101 22-23 13.89924277406735 1101 24-25 13.856829513582685 1101 26-27 13.832340026835414 1101 28-29 13.573778358123896 1101 30-31 13.682331303087079 1101 32-33 13.616819615124637 1101 34-35 13.683585614104675 1101 36-37 14.016806965828597 1101 38-39 13.752349354674092 1101 40-41 13.879822501366213 1101 42-43 13.495417555360817 1101 44-45 13.291756234497136 1101 46-47 12.810791736606475 1101 48-49 12.671886019499656 1101 50-51 14.637062333266623 1101 52-53 13.132006675093457 1101 54-55 12.882305304348012 1101 56-57 12.893152119068272 1101 58-59 12.950894030127554 1101 60-61 12.911588006215538 1101 62-63 13.202894574673167 1101 64-65 12.97440529862611 1101 66-67 11.943795189924284 1101 68-69 12.22846354688198 1101 70-71 12.50349124918269 1101 72-73 12.060972469817536 1101 74-75 12.193182183093029 1101 76-77 12.434097611254096 1101 78-79 12.007150762077327 1101 80-81 11.595656561841718 1101 82-83 10.593828855167864 1101 84-85 10.694392746197412 1101 86-87 10.803310392788248 1101 88-89 10.523326837359363 1101 90-91 10.338903548163323 1101 92-93 10.21567977858501 1101 94-95 10.269015657567403 1101 96-97 10.369092208482805 1101 98-99 11.427321615285267 1101 100-101 10.91940233370773 1102 1 8.446447755178095 1102 2 9.944402669065848 1102 3 9.490693291826794 1102 4 11.508752208316928 1102 5 12.612479317205 1102 6 11.523378419177472 1102 7 11.057408618328875 1102 8 10.308493128520709 1102 9 10.0871182746972 1102 10-11 9.256848081819001 1102 12-13 9.240789419665134 1102 14-15 10.426584987959007 1102 16-17 11.714758626843032 1102 18-19 12.604563642465298 1102 20-21 13.276010802778835 1102 22-23 13.10533454621925 1102 24-25 12.877676032570026 1102 26-27 13.564578950886048 1102 28-29 12.699885953060601 1102 30-31 13.258082885365564 1102 32-33 12.959066450567672 1102 34-35 12.640626753345177 1102 36-37 13.215422472157716 1102 38-39 12.305316126825982 1102 40-41 11.630890539340896 1102 42-43 11.77548084650006 1102 44-45 12.184833766142702 1102 46-47 12.221551230277356 1102 48-49 11.744393930892063 1102 50-51 14.129230054785612 1102 52-53 12.484340535852951 1102 54-55 12.05959960814548 1102 56-57 12.039394207675866 1102 58-59 11.879849726330082 1102 60-61 11.542758892291488 1102 62-63 12.01412875188835 1102 64-65 11.668194855588133 1102 66-67 11.301627468405297 1102 68-69 11.45124835700857 1102 70-71 11.655864666904211 1102 72-73 11.429010444501081 1102 74-75 11.206235980561381 1102 76-77 11.620490016317383 1102 78-79 10.830014686127956 1102 80-81 9.97532428336071 1102 82-83 9.592286133648873 1102 84-85 9.804044644931587 1102 86-87 9.946704380130022 1102 88-89 10.08246449558721 1102 90-91 10.071577598796232 1102 92-93 10.248393386179945 1102 94-95 10.429300467693983 1102 96-97 10.352557398356218 1102 98-99 8.72882477984223 1102 100-101 8.86797828307482 1103 1 8.378334433900214 1103 2 10.04589453163366 1103 3 9.53537382226682 1103 4 11.920672039540555 1103 5 12.685565513708916 1103 6 11.52571416360183 1103 7 10.884940263898496 1103 8 10.76260705257134 1103 9 10.46053599621619 1103 10-11 9.325564175851547 1103 12-13 9.493464223763986 1103 14-15 10.317972872226637 1103 16-17 11.555626619609757 1103 18-19 12.820167922151857 1103 20-21 13.225603931169431 1103 22-23 13.448349916924489 1103 24-25 13.117692608820779 1103 26-27 13.303917407787797 1103 28-29 13.292825977171518 1103 30-31 12.724742017372794 1103 32-33 12.92455771036273 1103 34-35 13.351145137914191 1103 36-37 13.463086727733359 1103 38-39 13.011426735626468 1103 40-41 12.777590358509066 1103 42-43 12.572798507741766 1103 44-45 12.64934552021142 1103 46-47 12.396506022320757 1103 48-49 12.762213400452037 1103 50-51 15.014740904695191 1103 52-53 13.982155484617266 1103 54-55 13.673674351967062 1103 56-57 13.057289023830176 1103 58-59 12.7032749825085 1103 60-61 12.87646895859649 1103 62-63 13.094561241339825 1103 64-65 13.090625536721348 1103 66-67 12.612545189924287 1103 68-69 12.47965402307246 1103 70-71 12.412419820611259 1103 72-73 12.075258184103252 1103 74-75 11.516098849759697 1103 76-77 11.872788087444569 1103 78-79 11.84613885731542 1103 80-81 11.41738275231791 1103 82-83 11.236537188501195 1103 84-85 11.636059412864078 1103 86-87 10.659709202312058 1103 88-89 11.095499456406984 1103 90-91 11.288010691020467 1103 92-93 11.199757159537388 1103 94-95 11.106515657567401 1103 96-97 11.005699351339945 1103 98-99 10.799047805761456 1103 100-101 9.743807095612492 1104 1 8.05839395770974 1104 2 9.824465960205089 1104 3 8.585076203219199 1104 4 10.74775537287389 1104 5 12.152827418470821 1104 6 11.062618925506587 1104 7 10.472440263898495 1104 8 9.525107052571343 1104 9 9.45428599621619 1104 10-11 8.936278461565834 1104 12-13 8.645101128525894 1104 14-15 9.476901443655205 1104 16-17 10.528840905324042 1104 18-19 11.854840541199476 1104 20-21 11.89792535974086 1104 22-23 11.952367774067351 1104 24-25 12.644329513582687 1104 26-27 12.566715026835414 1104 28-29 12.3894033581239 1104 30-31 12.260456303087079 1104 32-33 12.291819615124634 1104 34-35 11.79921061410467 1104 36-37 11.923056965828597 1104 38-39 11.827349354674087 1104 40-41 11.24232250136621 1104 42-43 11.620417555360817 1104 44-45 11.382381234497132 1104 46-47 10.92641673660647 1104 48-49 10.625011019499663 1104 50-51 13.624562333266628 1104 52-53 12.353881675093454 1104 54-55 11.29168030434801 1104 56-57 10.48065211906827 1104 58-59 10.966519030127554 1104 60-61 10.586588006215543 1104 62-63 11.081019574673164 1104 64-65 11.158780298626105 1104 66-67 10.946920189924288 1104 68-69 10.634713546881986 1104 70-71 10.03474124918269 1104 72-73 10.060972469817536 1104 74-75 9.911932183093029 1104 76-77 9.321597611254091 1104 78-79 9.241525762077327 1104 80-81 8.94565656184172 1104 82-83 8.71882885516786 1104 84-85 8.222517746197408 1104 86-87 8.659560392788247 1104 88-89 8.720201837359362 1104 90-91 7.392028548163324 1104 92-93 7.518804778585007 1104 94-95 7.0627656575673985 1104 96-97 7.156592208482802 1104 98-99 7.2991966152852665 1104 100-101 6.23502733370773 1105 1 7.4256733694744455 1105 2 9.049465960205087 1105 3 8.082502673807436 1105 4 10.31135831405036 1105 5 10.787386242000231 1105 6 10.462618925506586 1105 7 10.159940263898495 1105 8 9.614077640806634 1105 9 8.931124231510307 1105 10-11 8.39951375568348 1105 12-13 8.521020246172952 1105 14-15 9.260724973066974 1105 16-17 10.688399728853451 1105 18-19 11.776715541199483 1105 20-21 12.020351830329098 1105 22-23 12.353286891714408 1105 24-25 12.707013337112098 1105 26-27 12.464876791541304 1105 28-29 12.546572475770958 1105 30-31 11.971485714851784 1105 32-33 12.16755490924228 1105 34-35 12.294982672928203 1105 36-37 12.829123142299185 1105 38-39 12.409150825262326 1105 40-41 11.842506324895623 1105 42-43 11.967476378890225 1105 44-45 11.988631234497134 1105 46-47 11.737262324841765 1105 48-49 11.161408078323188 1105 50-51 12.70875350973721 1105 52-53 11.43292579274052 1105 54-55 10.996643539642132 1105 56-57 11.692968295538865 1105 58-59 12.104570500715784 1105 60-61 11.616735065039073 1105 62-63 11.50362986879081 1105 64-65 11.176795004508463 1105 66-67 11.404456954630167 1105 68-69 11.093169429234923 1105 70-71 11.039520660947396 1105 72-73 10.921266587464594 1105 74-75 10.335461594857733 1105 76-77 10.667921140665861 1105 78-79 10.489136056194972 1105 80-81 10.466612444194663 1105 82-83 10.50982150222669 1105 84-85 10.190716275609176 1105 86-87 10.019303039847074 1105 88-89 10.316157719712304 1105 90-91 9.013535901104497 1105 92-93 9.410716543290892 1105 94-95 8.874162716390929 1105 96-97 8.519459855541626 1105 98-99 7.790924556461734 1105 100-101 7.134843510178321 1106 1 7.709836265402046 1106 2 9.508806619545748 1106 3 8.623743785636783 1106 4 10.964170208038723 1106 5 11.439228517371923 1106 6 10.464816727704392 1106 7 9.836863340821576 1106 8 9.192552107516399 1106 9 9.119876655556848 1106 10-11 8.630509230796605 1106 12-13 8.670890963690724 1106 14-15 9.081022322776082 1106 16-17 10.31936288334602 1106 18-19 12.033308947792886 1106 20-21 11.702252282817781 1106 22-23 12.238871894946474 1106 24-25 11.753910557538727 1106 26-27 12.378322169692556 1106 28-29 11.905234402079941 1106 30-31 11.868285973416743 1106 32-33 12.029869065674081 1106 34-35 12.071841108610162 1106 36-37 12.13112702077365 1106 38-39 12.320721607421344 1106 40-41 11.745996951915664 1106 42-43 11.301736236679503 1106 44-45 11.494125739991645 1106 46-47 10.575077450892188 1106 48-49 10.366837942576577 1106 50-51 13.134933212387498 1106 52-53 11.869060246522025 1106 54-55 11.241439919732624 1106 56-57 10.993873272914431 1106 58-59 10.618339085072602 1106 60-61 10.766120973248505 1106 62-63 11.420339629618212 1106 64-65 10.648546782142589 1106 66-67 9.7904298053089 1106 68-69 9.537804206222642 1106 70-71 9.897309930501368 1106 72-73 9.337620821465887 1106 74-75 8.911932183093029 1106 76-77 8.28327343542992 1106 78-79 8.418483179659741 1106 80-81 8.024983484918643 1106 82-83 8.177242316706323 1106 84-85 7.79013450443917 1106 86-87 7.464196381799237 1106 88-89 7.04925403516156 1106 90-91 7.625235965745738 1106 92-93 7.834601481881709 1106 94-95 7.543328844380591 1106 96-97 6.994023527164124 1106 98-99 5.842088098801748 1106 100-101 5.5066276084330035 1107 1 7.682845712095705 1107 2 8.206191106403917 1107 3 7.912671232458965 1107 4 10.436022916733542 1107 5 11.498222155312924 1107 6 10.700630621413023 1107 7 10.075729737582709 1107 8 8.225326350816957 1107 9 5.747085703818531 1107 10-11 7.919757993729576 1107 12-13 7.864965894607764 1107 14-15 8.459211385175674 1107 16-17 9.822335057370825 1107 18-19 11.01121846517609 1107 20-21 11.255783546875364 1107 22-23 10.965945990441622 1107 24-25 11.136855098378003 1107 26-27 11.561488418648285 1107 28-29 11.409523972158986 1107 30-31 11.483044022385325 1107 32-33 11.070547685300074 1107 34-35 11.56417771936783 1107 36-37 11.536708281618072 1107 38-39 11.294582541808591 1107 40-41 10.631704811307735 1107 42-43 10.276850303898826 1107 44-45 10.09243240408778 1107 46-47 10.555236181050915 1107 48-49 11.679141136458721 1107 50-51 13.324525783559022 1107 52-53 11.721608283280588 1107 54-55 11.33511963183339 1107 56-57 10.804939399770024 1107 58-59 10.955206895624624 1107 60-61 10.635345316157064 1107 62-63 11.264517381690709 1107 64-65 10.844854860029614 1107 66-67 11.072706008637738 1107 68-69 10.640159453314734 1107 70-71 10.126809962632983 1107 72-73 9.572229779759056 1107 74-75 9.422166101221684 1107 76-77 9.742723342248247 1107 78-79 9.169029417048083 1107 80-81 8.806584924414818 1107 82-83 8.635294498442715 1107 84-85 8.545050640934253 1107 86-87 8.505887147174214 1107 88-89 7.885077568353516 1107 90-91 7.761747115414785 1107 92-93 7.153234603146412 1107 94-95 7.309732031836406 1107 96-97 7.232359752342454 1107 98-99 6.570559919378832 1107 100-101 5.378101164117087 1108 1 6.873408325525833 1108 2 8.728776305032675 1108 3 8.144774479081267 1108 4 9.818660545287685 1108 5 11.570212475942085 1108 6 9.30054995998935 1108 7 8.564537965047922 1108 8 6.88473348935295 1108 9 7.477777375526532 1108 10-11 5.953232484554334 1108 12-13 4.631703140020143 1108 14-15 7.578338225264407 1108 16-17 9.025248951301048 1108 18-19 9.31637071361327 1108 20-21 9.417106394223623 1108 22-23 10.120866337285737 1108 24-25 10.534667157260845 1108 26-27 8.867648934881398 1108 28-29 10.671623185710104 1108 30-31 10.758301130673285 1108 32-33 10.737365592136133 1108 34-35 10.755496533644905 1108 36-37 11.04744633364469 1108 38-39 10.63693987191547 1108 40-41 11.03395324849265 1108 42-43 9.192256635820588 1108 44-45 9.207022039094838 1108 46-47 9.538952656146702 1108 48-49 10.25697941030425 1108 50-51 12.034547965450532 1108 52-53 11.260275353254372 1108 54-55 10.235250706646863 1108 56-57 11.08427999263149 1108 58-59 11.152474489897667 1108 60-61 10.664030534951173 1108 62-63 10.503325609155922 1108 64-65 10.36043259747668 1108 66-67 10.471022201418538 1108 68-69 10.415819868721066 1108 70-71 10.945876306653954 1108 72-73 10.195742584760065 1108 74-75 10.854460918725213 1108 76-77 9.818293013552946 1108 78-79 9.957797313801464 1108 80-81 9.8576536882785 1108 82-83 9.764554429880508 1108 84-85 9.609478953093962 1108 86-87 8.900472749110087 1108 88-89 8.779612756899596 1108 90-91 9.118573088393205 1108 92-93 8.731879491228685 1108 94-95 8.609389220785795 1108 96-97 8.905227265954068 1108 98-99 7.884433684250782 1108 100-101 7.671557506121523 1109 1 6.814934655384157 1109 2 8.727372936949273 1109 3 8.890163412521524 1109 4 10.268540256594818 1109 5 10.979862302191748 1109 6 9.482386367367052 1109 7 7.980870496456635 1109 8 8.579467517687618 1109 9 7.448908089239449 1109 10-11 6.489185438310017 1109 12-13 6.646772640153799 1109 14-15 7.722250280864511 1109 16-17 6.637852533231015 1109 18-19 6.615087634222736 1109 20-21 8.697780010903656 1109 22-23 10.234271843834794 1109 24-25 9.332047536838498 1109 26-27 9.898255724509834 1109 28-29 9.460115567426222 1109 30-31 7.354933047273125 1109 32-33 6.840947522101377 1109 34-35 8.586056544337229 1109 36-37 7.7389726635030165 1109 38-39 6.742392959325251 1109 40-41 8.007656803691795 1109 42-43 6.245417555360817 1109 44-45 6.203747513566903 1109 46-47 8.20090801567624 1109 48-49 8.981551717174074 1109 50-51 11.136626286754993 1109 52-53 10.64646888439578 1109 54-55 10.149311118301494 1109 56-57 9.392352700463618 1109 58-59 8.95583589059267 1109 60-61 9.415367075982985 1109 62-63 9.618301551417346 1109 64-65 10.223169833509829 1109 66-67 9.88725449224987 1109 68-69 10.015091453858727 1109 70-71 9.524130784066411 1109 72-73 8.788298051212887 1109 74-75 7.641583345883724 1109 76-77 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-20.59785604229026 2314 2 -19.150534039794913 2314 3 -19.9586737967808 2314 4 -21.37099462712611 2314 5 -20.65967258152918 2314 6 -21.83738107449341 2314 7 -22.240059736101504 2314 8 -22.86239294742866 2314 9 -24.53946400378381 2314 10-11 -25.13872153843417 2314 12-13 -25.16427387147411 2314 14-15 -25.298098556344794 2314 16-17 -23.908659094675958 2314 18-19 -22.623284458800523 2314 20-21 -22.22082464025914 2314 22-23 -21.88200722593265 2314 24-25 -21.790045486417316 2314 26-27 -21.514534973164583 2314 28-29 -21.388721641876103 2314 30-31 -21.55204369691292 2314 32-33 -21.420680384875364 2314 34-35 -21.172664385895327 2314 36-37 -20.6738180341714 2314 38-39 -20.68202564532591 2314 40-41 -20.704552498633788 2314 42-43 -20.629582444639183 2314 44-45 -20.261368765502866 2314 46-47 -19.889208263393527 2314 48-49 -19.806238980500343 2314 50-51 -16.994187666733378 2314 52-53 -19.202368324906544 2314 54-55 -20.12394469565199 2314 56-57 -20.15997288093173 2314 58-59 -19.93660596987245 2314 60-61 -19.70091199378446 2314 62-63 -19.38460542532684 2314 64-65 -19.084969701373893 2314 66-67 -18.824954810075713 2314 68-69 -18.371536453118015 2314 70-71 -18.03400875081731 2314 72-73 -17.764027530182464 2314 74-75 -17.33806781690697 2314 76-77 -17.040902388745906 2314 78-79 -16.749099237922675 2314 80-81 -16.39809343815828 2314 82-83 -16.034296144832137 2314 84-85 -15.71810725380259 2314 86-87 -15.274814607211752 2314 88-89 -14.898548162640637 2314 90-91 -14.542346451836679 2314 92-93 -14.118695221414992 2314 94-95 -13.643484342432599 2314 96-97 -13.074657791517197 2314 98-99 -12.391428384714734 2314 100-101 -11.26809766629227 2315 1 -20.59785604229026 2315 2 -19.150534039794913 2315 3 -19.9586737967808 2315 4 -21.37099462712611 2315 5 -20.65967258152918 2315 6 -21.83738107449341 2315 7 -22.240059736101504 2315 8 -22.86239294742866 2315 9 -24.53946400378381 2315 10-11 -25.13872153843417 2315 12-13 -25.16427387147411 2315 14-15 -25.298098556344794 2315 16-17 -23.908659094675958 2315 18-19 -22.623284458800523 2315 20-21 -22.22082464025914 2315 22-23 -21.88200722593265 2315 24-25 -21.790045486417316 2315 26-27 -21.514534973164583 2315 28-29 -21.388721641876103 2315 30-31 -21.55204369691292 2315 32-33 -21.420680384875364 2315 34-35 -21.172664385895327 2315 36-37 -20.6738180341714 2315 38-39 -20.68202564532591 2315 40-41 -20.704552498633788 2315 42-43 -20.629582444639183 2315 44-45 -20.261368765502866 2315 46-47 -19.889208263393527 2315 48-49 -19.806238980500343 2315 50-51 -16.994187666733378 2315 52-53 -19.202368324906544 2315 54-55 -20.12394469565199 2315 56-57 -20.15997288093173 2315 58-59 -19.93660596987245 2315 60-61 -19.70091199378446 2315 62-63 -19.38460542532684 2315 64-65 -19.084969701373893 2315 66-67 -18.824954810075713 2315 68-69 -18.371536453118015 2315 70-71 -18.03400875081731 2315 72-73 -17.764027530182464 2315 74-75 -17.33806781690697 2315 76-77 -17.040902388745906 2315 78-79 -16.749099237922675 2315 80-81 -16.39809343815828 2315 82-83 -16.034296144832137 2315 84-85 -15.71810725380259 2315 86-87 -15.274814607211752 2315 88-89 -14.898548162640637 2315 90-91 -14.542346451836679 2315 92-93 -14.118695221414992 2315 94-95 -13.643484342432599 2315 96-97 -13.074657791517197 2315 98-99 -12.391428384714734 2315 100-101 -11.26809766629227 2316 1 -20.59785604229026 2316 2 -19.150534039794913 2316 3 -19.9586737967808 2316 4 -21.37099462712611 2316 5 -20.65967258152918 2316 6 -21.83738107449341 2316 7 -22.240059736101504 2316 8 -22.86239294742866 2316 9 -24.53946400378381 2316 10-11 -25.13872153843417 2316 12-13 -25.16427387147411 2316 14-15 -25.298098556344794 2316 16-17 -23.908659094675958 2316 18-19 -22.623284458800523 2316 20-21 -22.22082464025914 2316 22-23 -21.88200722593265 2316 24-25 -21.790045486417316 2316 26-27 -21.514534973164583 2316 28-29 -21.388721641876103 2316 30-31 -21.55204369691292 2316 32-33 -21.420680384875364 2316 34-35 -21.172664385895327 2316 36-37 -20.6738180341714 2316 38-39 -20.68202564532591 2316 40-41 -20.704552498633788 2316 42-43 -20.629582444639183 2316 44-45 -20.261368765502866 2316 46-47 -19.889208263393527 2316 48-49 -19.806238980500343 2316 50-51 -16.994187666733378 2316 52-53 -19.202368324906544 2316 54-55 -20.12394469565199 2316 56-57 -20.15997288093173 2316 58-59 -19.93660596987245 2316 60-61 -19.70091199378446 2316 62-63 -19.38460542532684 2316 64-65 -19.084969701373893 2316 66-67 -18.824954810075713 2316 68-69 -18.371536453118015 2316 70-71 -18.03400875081731 2316 72-73 -17.764027530182464 2316 74-75 -17.33806781690697 2316 76-77 -17.040902388745906 2316 78-79 -16.749099237922675 2316 80-81 -16.39809343815828 2316 82-83 -16.034296144832137 2316 84-85 -15.71810725380259 2316 86-87 -15.274814607211752 2316 88-89 -14.898548162640637 2316 90-91 -14.542346451836679 2316 92-93 -14.118695221414992 2316 94-95 -13.643484342432599 2316 96-97 -13.074657791517197 2316 98-99 -12.391428384714734 2316 100-101 -11.26809766629227 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 37780.0 3 1362.0 4 6785.0 5 3093.0 6 1117.0 7 1260.0 8 1322.0 9 978.0 10 995.0 11 1056.0 12 1194.0 13 1340.0 14 1460.0 15 1568.0 16 1719.0 17 1780.0 18 1517.0 19 1494.0 20 1689.0 21 1763.0 22 1998.0 23 2142.0 24 2348.0 25 2664.0 26 3014.0 27 3306.0 28 3809.0 29 4333.0 30 5011.0 31 5609.0 32 6745.0 33 7756.0 34 9143.0 35 10889.0 36 11573.0 37 9083.0 38 3098.0 39 107.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.43509329098849 21.815799920603414 15.443429932512903 22.305676855895197 2 15.520852503264365 22.11355609888617 39.903252096642746 22.46233930120672 3 18.494897959183675 25.901681427287897 31.711269413425747 23.89215120010268 4 13.465605447668857 18.922049545003816 38.29777898681081 29.31456602051651 5 13.127245922786326 37.253294020086614 35.44642034460518 14.173039712521884 6 28.665929823982577 38.10191524109602 18.51564690938485 14.716508025536548 7 24.55089333615126 33.933767943944986 23.86727577181317 17.648062948090583 8 23.087767412120886 35.55300479935906 22.0547111525395 19.304516635980555 9 24.187389142002573 18.41755642889438 23.886203178798734 33.50885125030432 10-11 23.255763233081066 27.88888646558054 29.074247744505755 19.78110255683264 12-13 23.404711855283928 25.931206040019493 29.527287010030328 21.13679509466625 14-15 21.18448842227921 28.237679626201178 27.9264361471737 22.65139580434591 16-17 20.093643393450574 30.33749303487174 29.054305952528892 20.51455761914879 18-19 19.805751123929085 29.209856646025955 31.171855119178893 19.81253711086606 20-21 20.65871797898713 28.56368350406835 31.101317348513664 19.676281168430858 22-23 20.411861328141974 27.926440297923744 30.959995828372282 20.701702545561997 24-25 20.414519677132485 28.25303486101124 31.291512601108845 20.04093286074743 26-27 20.379610392997158 28.378202174914595 31.10510348664366 20.13708394544459 28-29 20.020599118723187 28.724557388895576 31.083073421737883 20.17177007064335 30-31 20.54472148903638 28.41358515063766 30.991979089255057 20.049714271070908 32-33 20.3768203730345 28.546730269301634 30.725288777836667 20.351160579827194 34-35 20.49885063113031 28.55220425225579 30.593663337178196 20.355281779435703 36-37 20.520791020844467 28.289470871191874 30.72966328166756 20.460074826296097 38-39 20.492582568178705 28.371676716458822 30.73823146498561 20.397509250376867 40-41 20.643477832754844 28.26928915474813 30.588993099147444 20.49823991334958 42-43 20.691803006394817 28.333194917365667 30.131633585250395 20.84336849098912 44-45 20.269410786216344 28.626934555358048 30.104046054240936 20.999608604184676 46-47 20.47940990444281 28.384245884545034 30.14025880784287 20.996085403169285 48-49 20.72661464359928 28.429178294449493 29.951833953259317 20.89237310869191 50-51 20.731296337901593 28.745917079999312 29.428131966887307 21.094654615211795 52-53 20.38257425742574 28.996435643564354 29.477227722772277 21.143762376237625 54-55 20.33890124564106 29.13802603900622 29.32143331174812 21.201639403604595 56-57 20.14421843048622 29.176497859904458 29.474124299398124 21.205159410211195 58-59 19.917869525659626 28.892386127982554 29.69524839960328 21.494495946754537 60-61 20.379202900026474 28.91595218214773 29.239761318045744 21.465083599780055 62-63 20.074712106369226 28.714569673713775 29.40600217063465 21.804716049282348 64-65 19.899459054660177 28.7270655906183 29.703396244453117 21.6700791102684 66-67 20.004971474162367 28.675382783464237 29.321674424571913 21.997971317801486 68-69 20.041391199694242 28.41070804160263 29.315784765761588 22.23211599294154 70-71 20.052644363827508 28.31406623951194 29.357396294187215 22.27589310247333 72-73 19.896253602305475 28.443207790917217 29.398787637881345 22.261750968895953 74-75 19.747165185412197 28.779497395035243 29.10128715905608 22.372050260496476 76-77 19.776451112015454 28.71406050596802 29.19450491704827 22.314983464968254 78-79 19.81003858591224 28.62660709543374 28.990986205926923 22.572368112727105 80-81 19.622668397884535 28.379041707571968 29.035373848053965 22.962916046489536 82-83 20.052646761863553 27.81463852428803 29.37120374376973 22.761510970078692 84-85 19.891045913067565 28.09376514854228 29.17253372113602 22.84265521725414 86-87 19.62245190363848 28.101778613458738 29.231518082504742 23.04425140039804 88-89 20.08657506715365 27.589232226022766 29.112798809592828 23.211393897230757 90-91 19.934644368863104 27.753782663104666 29.058933832327106 23.252639135705124 92-93 19.955135847399134 27.22869737089791 28.92678986007404 23.889376921628912 94-95 19.80755981824234 27.152566514169823 29.118769497534508 23.921104170053333 96-97 19.93135413326003 27.148333104259255 28.950382553717873 23.969930208762847 98-99 19.93083888189526 26.931127053220692 28.640817193211287 24.49721687167276 100-101 20.25208164782119 26.945871350100063 28.569382454157292 24.23266454792145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10609.0 1 9611.0 2 6426.5 3 3334.5 4 2072.5 5 1597.0 6 1471.0 7 1532.5 8 1664.5 9 1689.0 10 1538.5 11 1295.5 12 1046.0 13 806.5 14 633.5 15 541.0 16 476.5 17 491.0 18 574.5 19 667.0 20 734.5 21 828.5 22 913.0 23 984.5 24 1111.5 25 1258.0 26 1394.5 27 1528.0 28 1708.5 29 1954.5 30 2148.5 31 2378.0 32 2663.0 33 2960.0 34 3346.5 35 3630.5 36 3930.5 37 4243.0 38 4346.0 39 4395.5 40 4472.5 41 4493.5 42 4516.5 43 4621.0 44 4730.5 45 4735.0 46 4695.0 47 4662.5 48 4560.5 49 4378.0 50 4080.0 51 3822.0 52 3599.0 53 3240.0 54 2936.5 55 2553.5 56 2078.5 57 1647.0 58 1247.5 59 1011.0 60 818.5 61 612.5 62 427.5 63 275.0 64 184.5 65 111.0 66 66.0 67 46.0 68 21.5 69 8.0 70 3.0 71 4.5 72 6.0 73 3.0 74 1.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 23.154362416107382 2 30.377669310555216 3 23.94386821232459 4 30.47162904209884 5 33.78279438682123 6 26.02928615009152 7 24.940817571690054 8 20.79987797437462 9 12.284929835265405 10-11 16.07504575960952 12-13 16.117754728492983 14-15 20.412141549725444 16-17 23.899633923123854 18-19 28.071995118974986 20-21 30.226662599145822 22-23 29.796827333740083 24-25 28.304148871262964 26-27 29.811470408785844 28-29 26.544539353264184 30-31 28.572910311165344 32-33 27.478340451494816 34-35 25.41732763880415 36-37 28.653142159853566 38-39 28.76632092739475 40-41 25.64429530201342 42-43 26.534472239170228 44-45 25.175106772422208 46-47 28.61622940817572 48-49 23.806894447834047 50-51 29.392312385600977 52-53 22.97132397803539 54-55 24.152837095790115 56-57 26.30323367907261 58-59 25.563758389261743 60-61 25.102196461256867 62-63 24.950884685784015 64-65 23.416107382550337 66-67 23.91000610128127 68-69 24.97071384990848 70-71 23.392617449664428 72-73 23.253508236729715 74-75 20.36607687614399 76-77 22.788590604026844 78-79 21.887736424649177 80-81 22.9359365466748 82-83 23.85967053081147 84-85 20.705918242830997 86-87 22.28523489932886 88-89 21.069249542403906 90-91 22.235204392922515 92-93 22.211104331909702 94-95 21.181818181818183 96-97 20.096400244051253 98-99 19.54484441732764 100-101 21.193105552165957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 163900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.75486198658487 #Duplication Level Percentage of deduplicated Percentage of total 1 93.25226846154358 72.50817264163283 2 4.632270609251137 7.203631238136711 3 0.9918050479446914 2.31352993861613 4 0.42293940894014714 1.315423814833157 5 0.21253901024541635 0.826297070419885 6 0.10763467626426955 0.5021471638739412 7 0.0946464008503535 0.5151452484952359 8 0.05279771027103551 0.32842229402656486 9 0.027892390170417475 0.19518920533591086 >10 0.17034115471696473 2.6497444496271916 >50 0.008965319091941206 0.5545795093780682 >100 0.021915224446967394 4.0377415955137845 >500 0.0029884396973137357 1.278785991596635 >1k 0.0 0.0 >5k 9.961465657712452E-4 5.771189838513948 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7451 4.546064673581452 No Hit NNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 619 0.3776693105552166 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT 518 0.31604636973764494 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA 514 0.3136058572300183 No Hit NNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 446 0.27211714460036607 No Hit NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 423 0.25808419768151314 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNN 420 0.25625381330079317 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNG 404 0.24649176327028677 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNN 351 0.2141549725442343 No Hit NNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 346 0.21110433190970104 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 336 0.2050030506406345 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNN 287 0.17510677242220865 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 276 0.1683953630262355 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNN 231 0.14093959731543623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.13971934106162293 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 193 0.11775472849298353 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNANNNNNNNNNNNNNNN 164 0.10006101281269067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 9.151921903599756E-4 0.0 28-29 0.0 0.0 0.0 0.003965832824893227 0.0 30-31 0.0 0.0 0.0 0.018608907870652835 0.0 32-33 0.0 0.0 0.0 0.04697986577181208 0.0 34-35 0.0 0.0 0.0 0.0896888346552776 0.0 36-37 0.0 0.0 0.0 0.18090298962782184 0.0 38-39 0.0 0.0 0.0 0.2830994508846858 0.0 40-41 0.0 0.0 0.0 0.37858450274557653 0.0 42-43 0.0 0.0 0.0 0.47742525930445395 0.0 44-45 0.0 0.0 0.0 0.6192800488102501 0.0 46-47 0.0 0.0 0.0 0.7879804758999389 0.0 48-49 0.0 0.0 0.0 0.9392922513727883 0.0 50-51 0.0 0.0 0.0 1.0991458206223306 0.0 52-53 0.0 0.0 0.0 1.2745576571079926 0.0 54-55 0.0 0.0 0.0 1.485051860890787 0.0 56-57 0.0 0.0 0.0 1.7461866992068336 0.0 58-59 0.0 0.0 0.0 2.0305064063453324 0.0 60-61 0.0 0.0 0.0 2.301403294691885 0.0 62-63 0.0 0.0 0.0 2.5869432580841973 0.0 64-65 0.0 0.0 0.0 2.9359365466748018 0.0 66-67 0.0 0.0 0.0 3.343502135448444 0.0 68-69 0.0 0.0 0.0 3.7849298352654057 0.0 70-71 0.0 0.0 0.0 4.24222086638194 0.0 72-73 0.0 0.0 0.0 4.707748627211714 0.0 74-75 0.0 0.0 0.0 5.206833435021354 0.0 76-77 0.0 0.0 0.0 5.812385600976205 0.0 78-79 0.0 0.0 0.0 6.412751677852349 0.0 80-81 0.0 0.0 0.0 7.065893837705918 0.0 82-83 0.0 0.0 0.0 7.758389261744966 0.0 84-85 0.0 0.0 0.0 8.429530201342281 0.0 86-87 0.0 0.0 0.0 9.130262355094569 0.0 88-89 0.0 0.0 0.0 9.87248322147651 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGCGT 25 5.676085E-5 74.827866 6 ATACGGG 30 9.477796E-5 67.465355 3 CTAAGTC 25 0.003645859 61.759716 2 TACGGGC 35 2.0317902E-4 57.827442 4 ACGGGCG 35 2.0365002E-4 57.800385 5 GTATACG 45 9.368781E-6 57.184925 1 GGCGTCG 25 0.0066811447 53.0018 8 GGTATAT 25 0.0066811447 53.0018 8 CCTCTCG 25 0.007067347 52.254395 9 GGTATCA 190 0.0 48.757668 1 GTATCAA 405 0.0 48.28949 1 GCTAAGT 45 6.45766E-4 45.74794 1 CGATCTC 20 7.8467093E-4 43.997353 92-93 TATACGG 60 5.134718E-5 42.88869 2 GTATTAC 50 0.0010848325 41.173145 1 TATCAAC 485 0.0 40.32421 2 ATCAACG 465 0.0 40.261585 3 CAACGCA 500 0.0 36.414246 5 TCAACGC 510 0.0 35.716953 4 GACCGTC 30 0.0031908131 33.067005 22-23 >>END_MODULE