FastQCFastQC Report
Sat 18 Jun 2016
SRR1930972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163900
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.5204392922513729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.45393532641854795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.39841366687004265No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.3233679072605247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.2940817571690055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.1513117754728493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.137888956680903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3150.054.3126031
GTGGTAT1550.052.122581
GTATCAA6000.042.771171
TGGTATC1750.040.734452
ACCTTGT500.001619962337.960838
TATCAAC7250.035.396832
ATCAACG7400.034.6687363
TCAACGC7450.034.436064
CAACGCA7550.033.948855
AACGCAG8050.031.8305116
AAAGGCG753.0801166E-431.6436795
ACAGGTC753.0856373E-431.6340268
ATCTATG650.00581554129.2452471
ACGCAGA8700.028.9069567
CGCAGAG8800.028.5784668
ATCCTGC908.88893E-426.4019581
GCAGAGT9300.026.0215369
GGCCTGG950.001218541425.012381
ACATGGG3450.023.4102363
GAGTACA2653.092282E-1123.3134271
Produced by FastQC (version 0.11.5)