FastQCFastQC Report
Sat 18 Jun 2016
SRR1930971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764209
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49130.177736198673834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29400.046.1427651
GGTATCA16400.042.2787781
ATCAACG37850.035.8267863
TCAACGC37650.035.3382454
TATCAAC38700.034.9151462
CAACGCA39050.033.003175
AACGCAG43550.030.373116
GTACATG64950.026.3792511
ACGCAGA49200.026.2446127
TACATGG66100.025.1535382
CGCAGAG54100.024.3114648
ACATGGG69450.023.936783
GCAGAGT60350.020.8574289
GAGTACA46200.020.3369961
CATGGGG57350.019.960234
AGTACAT46300.019.7452642
AGAGTAC53200.017.734810-11
GTATTAG5101.8189894E-1216.7481161
ATTAGAC5450.016.5876923
TAATACT12700.016.2085534
Produced by FastQC (version 0.11.5)