##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930971_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2764209 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.563152062669648 30.0 10.0 31.0 2.0 33.0 2 21.48400030533147 28.0 10.0 31.0 2.0 33.0 3 21.917251553699447 28.0 10.0 31.0 2.0 33.0 4 23.872445245638083 32.0 2.0 35.0 2.0 37.0 5 23.829029932251867 32.0 10.0 35.0 2.0 37.0 6 24.522118262403456 32.0 11.0 35.0 2.0 37.0 7 24.554539833999527 32.0 11.0 35.0 2.0 37.0 8 25.238418296156333 32.0 17.0 35.0 2.0 37.0 9 26.335645748928535 33.0 17.0 37.0 2.0 39.0 10-11 26.48629915465871 33.0 17.0 37.0 2.0 39.0 12-13 26.34205734805147 33.0 17.0 37.0 2.0 39.0 14-15 26.544567179978067 33.0 14.0 38.0 2.0 40.0 16-17 25.678523223099265 32.0 10.0 38.0 2.0 40.0 18-19 25.057806048674323 32.0 10.0 38.0 2.0 40.0 20-21 24.529256109071348 32.0 8.0 38.0 2.0 40.0 22-23 24.405761105618282 31.0 9.5 37.0 2.0 39.0 24-25 24.067824828006856 31.0 7.0 37.0 2.0 39.0 26-27 23.691863024829164 30.0 4.0 37.0 2.0 39.0 28-29 23.574012855033754 30.0 2.0 37.0 2.0 39.0 30-31 23.44769570607722 30.0 2.0 37.0 2.0 39.0 32-33 23.095592084390145 30.0 2.0 37.0 2.0 39.0 34-35 20.346167203709996 24.5 2.0 35.0 2.0 39.0 36-37 18.741054493346923 17.0 2.0 34.0 2.0 38.0 38-39 17.825272256909663 15.5 2.0 33.0 2.0 38.0 40-41 18.982859834404707 19.5 2.0 34.0 2.0 38.0 42-43 18.864604666289704 19.5 2.0 34.0 2.0 38.0 44-45 17.004535112938278 12.0 2.0 32.0 2.0 38.0 46-47 17.726913558272912 17.5 2.0 32.5 2.0 38.0 48-49 17.15694923936649 13.5 2.0 33.0 2.0 38.0 50-51 15.316038150516114 9.5 2.0 30.5 2.0 36.5 52-53 17.99885772023751 18.5 2.0 32.0 2.0 37.5 54-55 19.45748801917655 24.0 2.0 34.5 2.0 38.0 56-57 19.60943655128827 25.0 2.0 35.0 2.0 38.0 58-59 19.22639912539175 23.0 2.0 34.5 2.0 38.0 60-61 18.859294467241803 22.5 2.0 34.0 2.0 38.0 62-63 18.36220886336742 21.5 2.0 34.0 2.0 38.0 64-65 17.839550844382607 19.5 2.0 33.5 2.0 37.0 66-67 17.382112025537864 18.0 2.0 33.0 2.0 36.5 68-69 16.899774402007953 13.5 2.0 32.0 2.0 36.0 70-71 16.306493828795148 8.0 2.0 32.0 2.0 35.5 72-73 15.80890627300613 7.0 2.0 31.0 2.0 35.0 74-75 15.268007773652426 2.0 2.0 31.0 2.0 35.0 76-77 14.703627149756041 2.0 2.0 30.5 2.0 34.0 78-79 14.199008468607113 2.0 2.0 30.0 2.0 34.0 80-81 13.683481603597992 2.0 2.0 29.5 2.0 34.0 82-83 13.201657508531373 2.0 2.0 29.0 2.0 34.0 84-85 12.68773236755976 2.0 2.0 29.0 2.0 34.0 86-87 12.13310824181529 2.0 2.0 27.5 2.0 34.0 88-89 11.592901622127705 2.0 2.0 26.5 2.0 33.0 90-91 11.030629196272786 2.0 2.0 25.5 2.0 33.0 92-93 10.431959197007172 2.0 2.0 24.5 2.0 32.0 94-95 9.805802672663319 2.0 2.0 21.5 2.0 32.0 96-97 9.126280067824105 2.0 2.0 17.0 2.0 32.0 98-99 8.391495722646154 2.0 2.0 2.0 2.0 31.5 100-101 7.472324632471713 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.909616275219722 1101 2 8.03303502997792 1101 3 7.492295385144228 1101 4 9.415943874549871 1101 5 9.356051784272996 1101 6 8.669279985611194 1101 7 8.49202571725726 1101 8 7.6393971479574105 1101 9 7.738300813854227 1101 10-11 7.385683539832687 1101 12-13 7.29493548690245 1101 14-15 7.785450640783619 1101 16-17 8.363442198978387 1101 18-19 8.776621280135195 1101 20-21 9.15442728870488 1101 22-23 9.035882073056154 1101 24-25 9.208702880761795 1101 26-27 8.944706491505684 1101 28-29 8.937228861083078 1101 30-31 9.062427381400735 1101 32-33 8.985823527286751 1101 34-35 11.148008041120196 1101 36-37 5.560533742266411 1101 38-39 8.521695434776035 1101 40-41 10.11954315248645 1101 42-43 10.385727129634894 1101 44-45 4.832011059621323 1101 46-47 6.52878497449856 1101 48-49 9.378267654584796 1101 50-51 -2.756184058343221 1101 52-53 6.4022460567989015 1101 54-55 8.911753965795132 1101 56-57 9.354034804030523 1101 58-59 9.512959323833485 1101 60-61 9.378193843059798 1101 62-63 9.243466001396811 1101 64-65 9.0841041041039 1101 66-67 8.946801900983186 1101 68-69 8.952694760914309 1101 70-71 9.03170025014703 1101 72-73 8.955356772262247 1101 74-75 9.032180236337004 1101 76-77 8.906771749775432 1101 78-79 8.911831568554758 1101 80-81 8.56215798104318 1101 82-83 8.4139063321091 1101 84-85 8.184434025659927 1101 86-87 7.736076101800068 1101 88-89 7.580950381474963 1101 90-91 7.338654730510832 1101 92-93 7.375377049436677 1101 94-95 7.072441008344542 1101 96-97 6.842012122156381 1101 98-99 6.400679516262519 1101 100-101 6.024951284756231 1102 1 6.538969799295671 1102 2 7.361867157050856 1102 3 7.289260182928221 1102 4 9.029355641009285 1102 5 8.898723871717156 1102 6 8.519611238526704 1102 7 8.141934342362614 1102 8 7.289004062406434 1102 9 7.246744520188567 1102 10-11 6.771461369933373 1102 12-13 6.591767885992407 1102 14-15 6.894979818451485 1102 16-17 7.7811488416665675 1102 18-19 8.001562993269495 1102 20-21 8.360320416292737 1102 22-23 8.261026750943056 1102 24-25 8.376700171770398 1102 26-27 8.34446210703851 1102 28-29 8.23151424466317 1102 30-31 7.9213695565913405 1102 32-33 8.072326278082134 1102 34-35 10.044526642496255 1102 36-37 1.118291362527394 1102 38-39 3.476213921569588 1102 40-41 7.988126036980063 1102 42-43 9.209227734802887 1102 44-45 1.4487004215434567 1102 46-47 1.9811377470343459 1102 48-49 7.052506897744021 1102 50-51 -4.11048779608528 1102 52-53 4.7067004635561 1102 54-55 7.508243327670687 1102 56-57 8.281799788313858 1102 58-59 8.24790045706849 1102 60-61 8.327079621297457 1102 62-63 8.410270190365729 1102 64-65 8.238616407633902 1102 66-67 8.152451482512486 1102 68-69 8.057570479645918 1102 70-71 7.744438285415619 1102 72-73 7.822588479758247 1102 74-75 7.684844722209702 1102 76-77 7.489268246508319 1102 78-79 7.351885177799053 1102 80-81 7.634831123484846 1102 82-83 7.405816620523993 1102 84-85 7.25917390356434 1102 86-87 7.052561846874015 1102 88-89 6.838479640189 1102 90-91 6.661095272784209 1102 92-93 6.623681193341133 1102 94-95 6.366041749777228 1102 96-97 6.203538342570747 1102 98-99 5.715125715930929 1102 100-101 4.898772534641822 1103 1 6.7668830453902125 1103 2 7.397480251915873 1103 3 7.185659235097702 1103 4 8.774380529481245 1103 5 8.954362193696216 1103 6 8.051588120865912 1103 7 7.844320173485588 1103 8 7.162135188527998 1103 9 7.367808150234364 1103 10-11 6.776763988543625 1103 12-13 6.799734079639212 1103 14-15 7.148105197988915 1103 16-17 7.867994180493479 1103 18-19 8.196179858196757 1103 20-21 8.491181593467758 1103 22-23 8.111646385618261 1103 24-25 8.2456824156749 1103 26-27 8.224369691037246 1103 28-29 8.361249522973019 1103 30-31 8.162398468360955 1103 32-33 8.14109019541122 1103 34-35 10.010200961555885 1103 36-37 -2.7182827182429925 1103 38-39 -1.5124936273481246 1103 40-41 6.438539019622532 1103 42-43 8.737914680506051 1103 44-45 1.1024852577704038 1103 46-47 1.091058559885571 1103 48-49 7.311504044196511 1103 50-51 -4.38819273711449 1103 52-53 4.493051224215119 1103 54-55 7.533784001341157 1103 56-57 8.065410991834081 1103 58-59 8.295263571620666 1103 60-61 8.196250643819795 1103 62-63 8.160569400744205 1103 64-65 8.035151169187394 1103 66-67 7.9232256252256725 1103 68-69 7.933890351554261 1103 70-71 7.7751735566082445 1103 72-73 7.706382699241027 1103 74-75 7.694960650303704 1103 76-77 7.66071206257079 1103 78-79 7.3611037617424575 1103 80-81 7.1341927544402655 1103 82-83 6.676176758928737 1103 84-85 6.544802266022041 1103 86-87 6.331156268669615 1103 88-89 6.093928448869072 1103 90-91 5.979398062896596 1103 92-93 5.854195210713124 1103 94-95 5.574841519546522 1103 96-97 5.357292299230528 1103 98-99 5.0398617874668314 1103 100-101 4.468519834172016 1104 1 6.4707283859550415 1104 2 7.529965167089088 1104 3 7.025852769842658 1104 4 8.742366924325399 1104 5 8.803574054714378 1104 6 8.148686202053899 1104 7 7.976917329190261 1104 8 7.184765685683402 1104 9 7.225534257281769 1104 10-11 6.76259913325875 1104 12-13 6.478603897659223 1104 14-15 6.702698190772448 1104 16-17 7.3334431376085725 1104 18-19 8.00764883621666 1104 20-21 8.231035099531255 1104 22-23 7.973987883448114 1104 24-25 7.857727027277566 1104 26-27 7.928883965126623 1104 28-29 7.7116850062135995 1104 30-31 7.625579064378218 1104 32-33 7.413624736933702 1104 34-35 9.527293591060914 1104 36-37 -2.9394632080326115 1104 38-39 -1.94683842825647 1104 40-41 5.69935043849317 1104 42-43 7.769351048642967 1104 44-45 0.5311353549286117 1104 46-47 0.14629944109400128 1104 48-49 6.604019905687686 1104 50-51 -4.623509019908518 1104 52-53 3.7293200483051443 1104 54-55 6.390901804793959 1104 56-57 6.872466010739597 1104 58-59 6.941161230303223 1104 60-61 6.964697425272448 1104 62-63 7.099493515121313 1104 64-65 6.914750650876655 1104 66-67 6.804868986949849 1104 68-69 6.853174377221265 1104 70-71 6.8140263180760705 1104 72-73 6.673079330121004 1104 74-75 6.648112755264632 1104 76-77 6.47972771310989 1104 78-79 6.263774685532139 1104 80-81 6.044664896240144 1104 82-83 5.8910893165396985 1104 84-85 5.780211748543083 1104 86-87 5.621367190675473 1104 88-89 5.363397420876083 1104 90-91 5.025013316384822 1104 92-93 4.894868874374353 1104 94-95 4.619096858738411 1104 96-97 4.45602688745309 1104 98-99 3.973580246123733 1104 100-101 3.6504857872093766 1105 1 6.013422734718311 1105 2 6.840565315871245 1105 3 6.787703566854425 1105 4 8.576826396099037 1105 5 8.422889226020963 1105 6 7.687713020948898 1105 7 7.58906245972258 1105 8 6.730045662885601 1105 9 6.7645120343968195 1105 10-11 6.273641486468922 1105 12-13 5.916150847707851 1105 14-15 6.088538536583268 1105 16-17 6.602411454299183 1105 18-19 7.482409384755599 1105 20-21 7.632552412439345 1105 22-23 7.598299848814012 1105 24-25 7.709538508024522 1105 26-27 7.571359282696999 1105 28-29 7.333485237173875 1105 30-31 7.16444500635318 1105 32-33 7.0845404761971125 1105 34-35 8.888854445116884 1105 36-37 -3.4796831007702878 1105 38-39 -2.153852396879671 1105 40-41 5.114255494601274 1105 42-43 7.109254323672914 1105 44-45 -3.0823170041301164 1105 46-47 -3.9461222784499785 1105 48-49 4.427271839607201 1105 50-51 -8.976603042610222 1105 52-53 0.35219174274834586 1105 54-55 4.914201455039912 1105 56-57 6.01178708031431 1105 58-59 6.413410707163287 1105 60-61 6.1477524962090655 1105 62-63 6.205154195708555 1105 64-65 6.129715683756185 1105 66-67 6.077220523730357 1105 68-69 5.9509968227824 1105 70-71 6.029985441089757 1105 72-73 5.971373305769111 1105 74-75 5.748328638505436 1105 76-77 5.56669469440609 1105 78-79 5.444554449833371 1105 80-81 5.370257746743897 1105 82-83 5.4182571371793244 1105 84-85 5.074003489973615 1105 86-87 4.626042893621458 1105 88-89 4.755380083944143 1105 90-91 4.408750498835154 1105 92-93 4.264732700594143 1105 94-95 4.074866806399566 1105 96-97 3.9412076544599 1105 98-99 3.7062457165686062 1105 100-101 3.3888886488635066 1106 1 5.597755242232594 1106 2 6.714019412702708 1106 3 6.379294254825215 1106 4 7.5012398806876455 1106 5 7.6475715197250125 1106 6 6.672283912666302 1106 7 6.700517606380689 1106 8 5.947281208544702 1106 9 5.942966850058628 1106 10-11 5.621145640639643 1106 12-13 5.563542329401081 1106 14-15 5.795972078210237 1106 16-17 6.1983764756802096 1106 18-19 6.479443624483842 1106 20-21 6.9349556461728845 1106 22-23 6.705039134062613 1106 24-25 6.784964540381917 1106 26-27 6.933678530298948 1106 28-29 6.9153020056469785 1106 30-31 6.537320862680726 1106 32-33 6.389447078403087 1106 34-35 8.520314473134551 1106 36-37 -0.2123654747158632 1106 38-39 0.833945207242067 1106 40-41 5.768075994305384 1106 42-43 6.799447137375495 1106 44-45 -3.210098793984244 1106 46-47 -4.201638214751579 1106 48-49 3.722307563803696 1106 50-51 -8.979700198382432 1106 52-53 -0.014495485809611353 1106 54-55 4.131138936860193 1106 56-57 5.126767003215573 1106 58-59 5.498670840163619 1106 60-61 5.439493135533429 1106 62-63 5.44045896305828 1106 64-65 5.563204485048075 1106 66-67 5.526025092147961 1106 68-69 5.202475774426851 1106 70-71 5.186255647745121 1106 72-73 5.187044475991476 1106 74-75 5.231182027205422 1106 76-77 4.861222389434788 1106 78-79 4.853722880141932 1106 80-81 4.645433191499281 1106 82-83 4.5272724399072075 1106 84-85 3.9816362980592874 1106 86-87 4.04938849722163 1106 88-89 3.9514216691155113 1106 90-91 3.664039418483295 1106 92-93 3.387475155383056 1106 94-95 3.1224393330380806 1106 96-97 2.85655668838535 1106 98-99 2.787380933571786 1106 100-101 2.6243545679466145 1107 1 6.038538377348978 1107 2 7.170217726850698 1107 3 6.5498558502717295 1107 4 7.575118654420887 1107 5 8.03268534056025 1107 6 6.4484193805666195 1107 7 6.729912577765575 1107 8 6.292001637759423 1107 9 6.179616489964314 1107 10-11 5.75640830670395 1107 12-13 5.561012232917555 1107 14-15 5.830245762526285 1107 16-17 6.745541834884282 1107 18-19 6.868146674764979 1107 20-21 7.24862310326656 1107 22-23 7.059577028467054 1107 24-25 6.955014221563285 1107 26-27 7.0755308666787045 1107 28-29 6.917469795844511 1107 30-31 6.746032329364127 1107 32-33 6.565485818697365 1107 34-35 8.468409003584515 1107 36-37 -0.12301812944570756 1107 38-39 1.0808306262718972 1107 40-41 6.501799997331105 1107 42-43 7.454440676882349 1107 44-45 -3.041040197562065 1107 46-47 -3.9454438124218356 1107 48-49 4.502820672317537 1107 50-51 -8.840633479761587 1107 52-53 0.569194487666298 1107 54-55 5.022241271986861 1107 56-57 6.208709952125048 1107 58-59 6.403784785218157 1107 60-61 6.565633746548542 1107 62-63 6.28948462611147 1107 64-65 6.306787087285457 1107 66-67 6.339129094864223 1107 68-69 6.282042157546627 1107 70-71 6.193811504477992 1107 72-73 5.994783730531855 1107 74-75 6.013689727167437 1107 76-77 5.9946315728149475 1107 78-79 5.94469951405611 1107 80-81 5.863341244810714 1107 82-83 5.511968241011623 1107 84-85 5.366087888382298 1107 86-87 5.228194222380113 1107 88-89 4.943882701848601 1107 90-91 4.857069833251531 1107 92-93 4.679773951175031 1107 94-95 4.44312605218631 1107 96-97 4.171420954830078 1107 98-99 3.842547050731506 1107 100-101 3.4769771424286144 1108 1 5.870280262262604 1108 2 6.263817713998016 1108 3 6.442800875218065 1108 4 7.691614886431914 1108 5 7.798149243157027 1108 6 7.416035648434004 1108 7 7.512849898752901 1108 8 6.547627063381572 1108 9 4.914268491020948 1108 10-11 5.955365589131045 1108 12-13 5.354290662564583 1108 14-15 5.9638510350113485 1108 16-17 5.973133767902571 1108 18-19 6.826841421482435 1108 20-21 7.529882908764819 1108 22-23 7.3613930928117774 1108 24-25 7.062888172596967 1108 26-27 7.407935222120511 1108 28-29 7.44673014036853 1108 30-31 7.2537943221681545 1108 32-33 7.307773089044467 1108 34-35 9.195526611420544 1108 36-37 -3.177632138585322 1108 38-39 -1.9878636275408184 1108 40-41 5.379737056565247 1108 42-43 7.502570420339097 1108 44-45 -2.819504107481375 1108 46-47 -3.8395310976544437 1108 48-49 4.63102366513457 1108 50-51 -8.935692617947705 1108 52-53 0.7429850486170295 1108 54-55 5.142665932918128 1108 56-57 6.299528087653304 1108 58-59 6.552605513359769 1108 60-61 6.5640683095098 1108 62-63 6.643511816310731 1108 64-65 6.5895931491612885 1108 66-67 6.4435833689876745 1108 68-69 6.433254457301345 1108 70-71 6.462250704678301 1108 72-73 6.328836967085367 1108 74-75 6.244411823868353 1108 76-77 6.228542304159896 1108 78-79 6.2747342108339 1108 80-81 6.135104000032365 1108 82-83 6.049354901610425 1108 84-85 5.83492521571946 1108 86-87 5.697223957432337 1108 88-89 5.448416168605021 1108 90-91 5.368262104242909 1108 92-93 5.121773325808972 1108 94-95 4.971692778099909 1108 96-97 4.675687695640109 1108 98-99 4.552285558755852 1108 100-101 4.217252355954184 1109 1 4.8038646676577805 1109 2 6.362038221794823 1109 3 6.146390444808322 1109 4 7.619548561294053 1109 5 7.457001918314674 1109 6 5.015356866540387 1109 7 4.663684738645038 1109 8 3.1037998396714706 1109 9 2.633290073172624 1109 10-11 2.3771715953899175 1109 12-13 3.023694421588278 1109 14-15 3.5675387737318083 1109 16-17 2.446208346672403 1109 18-19 3.4213073422200324 1109 20-21 3.9212606799221277 1109 22-23 5.714093364561311 1109 24-25 5.533367493346123 1109 26-27 4.2981969386538985 1109 28-29 4.170022499210319 1109 30-31 4.199697281046195 1109 32-33 3.8520135632998667 1109 34-35 5.984677399595327 1109 36-37 -4.444697228707019 1109 38-39 -3.26179831547252 1109 40-41 2.741045143846467 1109 42-43 3.4955787001770915 1109 44-45 -4.367404861605575 1109 46-47 -5.737665769241865 1109 48-49 1.371845667525065 1109 50-51 -9.276720751374505 1109 52-53 -1.3959428906898275 1109 54-55 2.142325829351474 1109 56-57 3.2557443797290553 1109 58-59 4.248762630403327 1109 60-61 4.330343023769359 1109 62-63 4.31675889505787 1109 64-65 3.971668645256763 1109 66-67 4.031574218948901 1109 68-69 4.1366167931128714 1109 70-71 4.160599817368897 1109 72-73 4.048703520974199 1109 74-75 4.18438825684054 1109 76-77 4.05766100835036 1109 78-79 4.204831261610709 1109 80-81 4.0665481896577464 1109 82-83 3.8673098412785336 1109 84-85 3.799702255935701 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2313 78-79 -11.813149320255576 2313 80-81 -11.300119439968146 2313 82-83 -10.817871666783873 2313 84-85 -10.329355306841316 2313 86-87 -9.78986929293055 2313 88-89 -9.268553454946346 2313 90-91 -8.721626616749848 2313 92-93 -8.12337681371484 2313 94-95 -7.567846761439631 2313 96-97 -6.890891091214686 2313 98-99 -6.150381545715923 2313 100-101 -5.2534609263302245 2314 1 -20.15307642776075 2314 2 -19.035481585301284 2314 3 -19.435077002659813 2314 4 -21.338081476597782 2314 5 -21.27258816091371 2314 6 -21.997044097979074 2314 7 -22.028357975788307 2314 8 -22.74566622991172 2314 9 -23.833812923727134 2314 10-11 -24.00743054033175 2314 12-13 -23.86676372515647 2314 14-15 -24.05216498100467 2314 16-17 -23.184690723255255 2314 18-19 -22.53735610938176 2314 20-21 -22.00915613027422 2314 22-23 -21.860829129410444 2314 24-25 -21.541382765007302 2314 26-27 -21.16046651294789 2314 28-29 -21.05026685662847 2314 30-31 -20.93723003552287 2314 32-33 -20.579781131843088 2314 34-35 -17.936438687211425 2314 36-37 -16.33412196174181 2314 38-39 -15.448157254501112 2314 40-41 -16.581724404896214 2314 42-43 -16.451717200615192 2314 44-45 -14.611462732773333 2314 46-47 -15.28330820809822 2314 48-49 -14.769043068265496 2314 50-51 -12.96755781647957 2314 52-53 -15.588846783214802 2314 54-55 -16.985128528209668 2314 56-57 -17.12489633677112 2314 58-59 -16.73935311193219 2314 60-61 -16.37737313193164 2314 62-63 -15.898363392231143 2314 64-65 -15.390887331319192 2314 66-67 -14.965325539921235 2314 68-69 -14.48015895611207 2314 70-71 -13.886764977670715 2314 72-73 -13.418743947162215 2314 74-75 -12.896562483772621 2314 76-77 -12.345198102913846 2314 78-79 -11.843050240283885 2314 80-81 -11.359390494462131 2314 82-83 -10.881742862820673 2314 84-85 -10.38579550500126 2314 86-87 -9.846486419611372 2314 88-89 -9.325524438668921 2314 90-91 -8.786559383911914 2314 92-93 -8.189371152002172 2314 94-95 -7.567846761439631 2314 96-97 -6.9140687274496475 2314 98-99 -6.2036370305000705 2314 100-101 -5.310608838573678 2315 1 -20.15307642776075 2315 2 -19.035481585301284 2315 3 -19.435077002659813 2315 4 -16.842911530265045 2315 5 -21.27258816091371 2315 6 -21.997044097979074 2315 7 -22.028357975788307 2315 8 -22.74566622991172 2315 9 -19.67996676988098 2315 10-11 -18.132296372174327 2315 12-13 -15.122756569521407 2315 14-15 -15.058426161684455 2315 16-17 -19.523688934346488 2315 18-19 -22.53735610938176 2315 20-21 -22.00915613027422 2315 22-23 -21.860829129410444 2315 24-25 -21.541382765007302 2315 26-27 -21.16046651294789 2315 28-29 -21.05026685662847 2315 30-31 -20.93723003552287 2315 32-33 -20.579781131843088 2315 34-35 -17.936438687211425 2315 36-37 -16.33412196174181 2315 38-39 -15.448157254501112 2315 40-41 -16.581724404896214 2315 42-43 -16.451717200615192 2315 44-45 -14.611462732773333 2315 46-47 -15.28330820809822 2315 48-49 -14.769043068265496 2315 50-51 -12.96755781647957 2315 52-53 -15.588846783214802 2315 54-55 -16.985128528209668 2315 56-57 -17.12489633677112 2315 58-59 -16.73935311193219 2315 60-61 -16.37737313193164 2315 62-63 -15.898363392231143 2315 64-65 -15.390887331319192 2315 66-67 -14.965325539921235 2315 68-69 -14.48015895611207 2315 70-71 -13.886764977670715 2315 72-73 -13.418743947162215 2315 74-75 -12.896562483772621 2315 76-77 -12.345198102913846 2315 78-79 -11.843050240283885 2315 80-81 -11.359390494462131 2315 82-83 -10.881742862820673 2315 84-85 -10.38579550500126 2315 86-87 -9.846486419611372 2315 88-89 -9.325524438668921 2315 90-91 -8.786559383911914 2315 92-93 -8.189371152002172 2315 94-95 -7.567846761439631 2315 96-97 -6.9140687274496475 2315 98-99 -6.2036370305000705 2315 100-101 -5.310608838573678 2316 1 -20.15307642776075 2316 2 -19.035481585301284 2316 3 -19.435077002659813 2316 4 -21.338081476597782 2316 5 -21.27258816091371 2316 6 -21.997044097979074 2316 7 -22.028357975788307 2316 8 -22.74566622991172 2316 9 -23.833812923727134 2316 10-11 -24.00743054033175 2316 12-13 -23.86676372515647 2316 14-15 -24.05216498100467 2316 16-17 -23.184690723255255 2316 18-19 -22.53735610938176 2316 20-21 -22.00915613027422 2316 22-23 -21.860829129410444 2316 24-25 -21.541382765007302 2316 26-27 -21.16046651294789 2316 28-29 -21.05026685662847 2316 30-31 -20.93723003552287 2316 32-33 -20.579781131843088 2316 34-35 -17.936438687211425 2316 36-37 -16.33412196174181 2316 38-39 -15.448157254501112 2316 40-41 -16.581724404896214 2316 42-43 -16.451717200615192 2316 44-45 -14.611462732773333 2316 46-47 -15.28330820809822 2316 48-49 -14.769043068265496 2316 50-51 -12.96755781647957 2316 52-53 -15.588846783214802 2316 54-55 -16.985128528209668 2316 56-57 -17.12489633677112 2316 58-59 -16.73935311193219 2316 60-61 -16.37737313193164 2316 62-63 -15.898363392231143 2316 64-65 -15.390887331319192 2316 66-67 -14.965325539921235 2316 68-69 -14.48015895611207 2316 70-71 -13.886764977670715 2316 72-73 -13.418743947162215 2316 74-75 -12.896562483772621 2316 76-77 -12.345198102913846 2316 78-79 -11.843050240283885 2316 80-81 -11.359390494462131 2316 82-83 -10.881742862820673 2316 84-85 -10.38579550500126 2316 86-87 -9.846486419611372 2316 88-89 -9.325524438668921 2316 90-91 -8.786559383911914 2316 92-93 -8.189371152002172 2316 94-95 -7.567846761439631 2316 96-97 -6.9140687274496475 2316 98-99 -6.2036370305000705 2316 100-101 -5.310608838573678 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 473473.0 3 32207.0 4 61942.0 5 43186.0 6 39322.0 7 48354.0 8 60742.0 9 70381.0 10 71002.0 11 64636.0 12 54694.0 13 48173.0 14 46719.0 15 49166.0 16 53541.0 17 57686.0 18 60178.0 19 62794.0 20 65331.0 21 68782.0 22 71985.0 23 75799.0 24 79139.0 25 82716.0 26 86909.0 27 91255.0 28 95003.0 29 97981.0 30 99105.0 31 96515.0 32 91984.0 33 85901.0 34 75037.0 35 56942.0 36 33934.0 37 10850.0 38 843.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.672407654257775 18.451492921606537 12.90858348477148 24.96751593936421 2 16.37825235985711 19.85847700821673 38.97795033464576 24.785320297280407 3 19.689078747993246 24.194177551395452 28.82891356132607 27.28783013928523 4 13.56877203225115 15.877354725710022 36.410258068330464 34.14361517370836 5 14.691820595066918 36.699987095822735 34.07781218891716 14.530380120193193 6 33.22433778026051 34.978233732274475 17.32639463044467 14.471033857020338 7 28.924362331802172 31.070168438742098 21.51786755842439 18.487601671031342 8 26.73288553698534 34.47989601152482 20.072975229909208 18.714243221580638 9 27.20015498706268 15.366706469033323 19.560000670617097 37.8731378732869 10-11 26.38310606347092 25.019371125292594 27.533237686794372 21.064285124442115 12-13 26.558970304061152 22.573236011190524 27.385655409345937 23.482138275402388 14-15 23.749615431048245 23.826276087405223 24.816594685743883 27.607513795802646 16-17 24.103620857482788 26.647393872032143 25.506825900049474 23.742159370435598 18-19 24.122674325887445 26.11773527299228 26.691629824339202 23.067960576781065 20-21 24.799342167844436 25.420520050986216 26.580076056558344 23.200061724610997 22-23 24.586597543986848 25.15501817938591 26.378379784423778 23.880004492203465 24-25 24.463340785930313 25.364493176667334 26.26120545118804 23.910960586214312 26-27 24.260304033735782 25.542735807604856 26.241872035005585 23.955088123653777 28-29 24.249133166104343 25.764237713153925 26.24069153278221 23.745937587959514 30-31 24.497440817136233 25.60897538190618 26.0653040637158 23.82827973724179 32-33 24.377994842496335 25.690407416532114 25.91833289263502 24.01326484833653 34-35 24.64171914338082 25.567802345466784 26.100076747472357 23.690401763680036 36-37 25.130773540299206 25.560297894171956 25.64794436690881 23.660984198620028 38-39 24.939006578313922 25.569887795417394 25.61763343425661 23.87347219201207 40-41 24.881104649186053 25.482002991549823 25.87651115711208 23.76038120215204 42-43 25.06103688420237 25.41890957951492 25.576590936082955 23.94346260019975 44-45 25.05962176542261 25.609814367967125 25.511112297846967 23.8194515687633 46-47 24.58354635068537 25.459772046857697 25.749936223687502 24.20674537876943 48-49 24.986331553728316 25.52693862032648 25.466134050970606 24.020595774974595 50-51 25.089975113266544 25.750202831435683 25.441511618366956 23.718310436930818 52-53 24.335471932507627 25.627145829650523 25.80923219640514 24.22815004143671 54-55 24.10336855295207 25.527309215489645 26.029084087054628 24.340238144503655 56-57 24.206924441998705 25.646895355739357 25.79134179042587 24.35483841183607 58-59 24.231604527828882 25.629300353107563 25.93108643612468 24.208008682938875 60-61 24.220509333352027 25.70833640891822 25.88546947995743 24.185684777772327 62-63 24.20506389469908 25.637849745614265 25.79997416064865 24.35711219903801 64-65 24.035803724720132 25.53802451407786 25.83134930027253 24.59482246092948 66-67 24.185115614360754 25.636632743896286 25.637574303926165 24.540677337816796 68-69 24.210110298341935 25.538837119221043 25.778099453383803 24.47295312905322 70-71 24.007611557656734 25.386869859088286 25.717499444225762 24.888019139029222 72-73 24.194921759645403 25.51167437883085 25.668928802472323 24.624475059051427 74-75 24.16617699955514 25.61865333636663 25.560805706172967 24.654363957905254 76-77 24.155407769536566 25.39743100597736 25.638582252168867 24.8085789723172 78-79 24.130487567394038 25.484947816217264 25.56559251754058 24.818972098848118 80-81 24.286019464966948 25.492076816381676 25.437418019441342 24.784485699210034 82-83 24.33197196025699 25.423000925840572 25.628529516679162 24.61649759722328 84-85 24.31278952854172 25.475108103335536 25.43496601837366 24.777136349749078 86-87 24.0915602597275 25.50829217238576 25.489244687004703 24.910902880882038 88-89 24.174541552200598 25.27091207540349 25.43483535613264 25.119711016263274 90-91 24.190144445510125 25.35750719532524 25.361767715639793 25.09058064352484 92-93 24.299111603831935 25.12488704168086 25.50213036048235 25.073870994004853 94-95 24.12175645060373 25.081434677689796 25.488881779109985 25.30792709259649 96-97 24.11418452607059 25.043094761674116 25.457974512915722 25.384746199339574 98-99 23.982149255571105 25.026994245860024 25.310844204379222 25.68001229418965 100-101 24.583644372621066 24.99846203216279 24.958787504480277 25.459106090735865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29663.0 1 32987.5 2 24484.5 3 12748.5 4 16420.5 5 22050.0 6 24426.0 7 23795.0 8 22115.0 9 20430.5 10 18677.0 11 16763.5 12 14696.0 13 12617.5 14 10455.5 15 8654.5 16 7162.0 17 6060.5 18 5397.0 19 4911.5 20 4813.5 21 5073.0 22 5492.0 23 6216.5 24 7084.0 25 8061.5 26 9126.0 27 10510.0 28 12123.0 29 13882.5 30 16293.0 31 19332.5 32 23182.5 33 27627.5 34 32806.0 35 38649.5 36 45330.5 37 52655.0 38 59475.0 39 65720.5 40 71832.0 41 77300.0 42 82061.5 43 86882.5 44 91449.5 45 95554.0 46 98953.5 47 101855.5 48 103764.5 49 104457.0 50 103599.0 51 101646.0 52 98914.0 53 95967.0 54 91744.0 55 85164.5 56 77786.5 57 69467.0 58 61409.0 59 53680.0 60 45748.0 61 38169.5 62 31286.5 63 25133.0 64 19775.0 65 15481.0 66 11938.0 67 9210.0 68 7169.5 69 5562.0 70 4359.5 71 3396.0 72 2647.5 73 2094.0 74 1604.0 75 1149.0 76 860.0 77 653.5 78 455.5 79 311.5 80 218.5 81 141.5 82 85.0 83 46.5 84 31.5 85 18.5 86 9.0 87 8.0 88 6.5 89 4.0 90 1.5 91 3.5 92 4.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.940696958876844 2 20.70534463928017 3 16.532794734406842 4 21.67712354601262 5 21.502317661218814 6 15.551031054453551 7 14.407195693234485 8 11.579876919581695 9 2.8982613109211353 10-11 6.24855067037261 12-13 3.416890690971631 14-15 7.105070564490601 16-17 11.044841399474498 18-19 14.023541635238146 20-21 14.429589079552233 22-23 12.380937910266553 24-25 14.856582841601341 26-27 12.478723569744545 28-29 12.472718235126216 30-31 13.285988866977858 32-33 11.82217046540258 34-35 18.6409565991573 36-37 23.350983952371184 38-39 22.411854530536583 40-41 17.53805519047221 42-43 17.45135769400939 44-45 22.7733141741453 46-47 12.289935384770109 48-49 20.928880558597417 50-51 20.630458840123882 52-53 10.773317068282463 54-55 12.318623519422735 56-57 11.841344124123756 58-59 12.838627614626825 60-61 12.270996874693628 62-63 12.216062533621734 64-65 13.259688395486737 66-67 11.629167693180943 68-69 12.628477079699834 70-71 11.311518050914385 72-73 10.693764472946873 74-75 10.789632766552746 76-77 10.822571665167143 78-79 11.200853481050094 80-81 11.177790825512831 82-83 9.738011850768158 84-85 9.653032748247329 86-87 9.78368495291058 88-89 11.137978351130467 90-91 8.72014380967575 92-93 10.685805595741856 94-95 10.785074500517146 96-97 10.334222195210275 98-99 10.192264767244446 100-101 10.320800634105453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2764209.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.73987251095872 #Duplication Level Percentage of deduplicated Percentage of total 1 99.0912693948712 96.85168037603776 2 0.637083861845757 1.2453699087118706 3 0.12448944476042413 0.36502747379531675 4 0.04324099655497954 0.1690547796212202 5 0.027346350879001582 0.1336414424276779 6 0.01633317689742994 0.09578415765922431 7 0.012631822722713565 0.08642429197493386 8 0.00793273170679371 0.06202753485485257 9 0.007959181961097276 0.07001364871522531 >10 0.029219550989418062 0.48914215249549 >50 0.0017858651296937746 0.11916031378182168 >100 6.335628676525275E-4 0.10778820513964935 >500 3.702940690881012E-5 0.02707198633796789 >1k 3.70294069087771E-5 0.17781372844694873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4913 0.177736198673834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.235342913650886E-5 2 0.0 0.0 0.0 0.0 7.235342913650886E-5 3 0.0 0.0 0.0 0.0 7.235342913650886E-5 4 0.0 0.0 0.0 3.617671456825443E-5 7.235342913650886E-5 5 0.0 0.0 0.0 3.617671456825443E-5 7.235342913650886E-5 6 0.0 0.0 0.0 3.617671456825443E-5 1.0853014370476328E-4 7 0.0 0.0 0.0 3.617671456825443E-5 1.0853014370476328E-4 8 0.0 0.0 0.0 3.617671456825443E-5 1.447068582730177E-4 9 0.0 0.0 0.0 3.617671456825443E-5 1.8088357284127212E-4 10-11 0.0 0.0 0.0 3.617671456825443E-5 1.8088357284127212E-4 12-13 0.0 0.0 0.0 3.617671456825443E-5 3.798555029666715E-4 14-15 0.0 3.617671456825443E-5 0.0 3.617671456825443E-5 4.702972893873076E-4 16-17 0.0 3.617671456825443E-5 0.0 3.617671456825443E-5 5.788274330920707E-4 18-19 0.0 3.617671456825443E-5 0.0 3.617671456825443E-5 7.416226486492157E-4 20-21 0.0 3.617671456825443E-5 0.0 5.426507185238164E-5 8.863295069222335E-4 22-23 0.0 3.617671456825443E-5 0.0 1.0853014370476328E-4 9.40594578774615E-4 24-25 3.617671456825443E-5 3.617671456825443E-5 0.0 1.0853014370476328E-4 9.40594578774615E-4 26-27 3.617671456825443E-5 7.235342913650886E-5 0.0 3.0750207383016266E-4 9.767712933428695E-4 28-29 3.617671456825443E-5 7.235342913650886E-5 0.0 9.586829360587422E-4 9.767712933428695E-4 30-31 3.617671456825443E-5 7.235342913650886E-5 0.0 0.003888996816087351 9.767712933428695E-4 32-33 3.617671456825443E-5 7.235342913650886E-5 0.0 0.010889191085044582 9.767712933428695E-4 34-35 3.617671456825443E-5 7.235342913650886E-5 0.0 0.021199554736997094 9.948596506269966E-4 36-37 3.617671456825443E-5 7.235342913650886E-5 0.0 0.03700877900332428 0.0010129480079111239 38-39 3.617671456825443E-5 7.235342913650886E-5 0.0 0.05918510503366424 0.0010491247224793784 40-41 3.617671456825443E-5 7.235342913650886E-5 0.0 0.08333306200797408 0.0010491247224793784 42-43 3.617671456825443E-5 7.235342913650886E-5 3.617671456825443E-5 0.11001338900206172 0.0010672130797635057 44-45 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.14316934790386687 0.0011395665089000144 46-47 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.1821316694938769 0.001193831580752396 48-49 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.2243498953950298 0.001193831580752396 50-51 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.27872349739111624 0.001266185009888905 52-53 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.3467176324221504 0.0013928035108777955 54-55 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.4203191582112641 0.0014108918681619226 56-57 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.5051897305883889 0.0015013336545825588 58-59 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.5983809473162123 0.0015917754410031948 60-61 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.7034923914942757 0.0016279521555714492 62-63 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.8114075310513785 0.0016641288701397035 64-65 3.617671456825443E-5 1.0853014370476328E-4 3.617671456825443E-5 0.9237181414285245 0.0017183939419920854 66-67 5.426507185238164E-5 1.0853014370476328E-4 3.617671456825443E-5 1.050915469850507 0.0017364822992762124 68-69 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 1.1918780381657104 0.0017364822992762124 70-71 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 1.3418847851229772 0.001790747371128594 72-73 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 1.5001940880736586 0.0018992775148333574 74-75 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 1.656929703940621 0.001953542586685739 76-77 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 1.8308854359420725 0.001953542586685739 78-79 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 2.025082039744462 0.001971630943969866 80-81 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 2.23776494469123 0.0019897193012539936 82-83 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 2.4655697163275283 0.0020620727303905023 84-85 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 2.691601828949982 0.0020620727303905023 86-87 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 2.92479693105695 0.0020982494449587564 88-89 7.235342913650886E-5 1.0853014370476328E-4 3.617671456825443E-5 3.171847714843559 0.002134426159527011 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2940 0.0 46.142765 1 GGTATCA 1640 0.0 42.278778 1 ATCAACG 3785 0.0 35.826786 3 TCAACGC 3765 0.0 35.338245 4 TATCAAC 3870 0.0 34.915146 2 CAACGCA 3905 0.0 33.00317 5 AACGCAG 4355 0.0 30.37311 6 GTACATG 6495 0.0 26.379251 1 ACGCAGA 4920 0.0 26.244612 7 TACATGG 6610 0.0 25.153538 2 CGCAGAG 5410 0.0 24.311464 8 ACATGGG 6945 0.0 23.93678 3 GCAGAGT 6035 0.0 20.857428 9 GAGTACA 4620 0.0 20.336996 1 CATGGGG 5735 0.0 19.96023 4 AGTACAT 4630 0.0 19.745264 2 AGAGTAC 5320 0.0 17.7348 10-11 GTATTAG 510 1.8189894E-12 16.748116 1 ATTAGAC 545 0.0 16.587692 3 TAATACT 1270 0.0 16.208553 4 >>END_MODULE