FastQCFastQC Report
Sat 18 Jun 2016
SRR1930971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764209
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.047.8282171
GTATCAA39800.038.8947071
CAACGCA45250.033.1427655
TCAACGC46800.032.158714
ATCAACG47300.031.718973
AACGCAG49300.030.5152456
TATCAAC50850.029.8785842
GTACATG86200.027.9290831
TACATGG90000.026.0079062
ACGCAGA59050.025.2356387
ACATGGG88350.025.2034193
CGCAGAG62400.023.8047878
CATGGGG69550.022.2538784
CTAGTAC8700.020.737553
GAGTACA60900.020.4286651
GTACTAA4500.018.993981
GCAGAGT77400.018.8848829
AGTACAT63650.018.7230382
ATGGGGG41900.018.5759075
TATACTG10800.018.4563485
Produced by FastQC (version 0.11.5)