##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930971_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2764209 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20382105694613 31.0 31.0 33.0 30.0 34.0 2 31.321293360957874 31.0 31.0 34.0 30.0 34.0 3 31.410628863447013 31.0 31.0 34.0 28.0 34.0 4 35.277474315437075 37.0 35.0 37.0 33.0 37.0 5 35.04894130653652 35.0 35.0 37.0 32.0 37.0 6 35.08921792816679 36.0 35.0 37.0 32.0 37.0 7 34.95671745515625 36.0 35.0 37.0 32.0 37.0 8 34.932480141696956 36.0 35.0 37.0 32.0 37.0 9 36.444705519734576 38.0 35.0 39.0 32.0 39.0 10-11 36.36353473995635 38.0 35.0 39.0 32.0 39.0 12-13 36.247246680695994 38.0 35.0 39.0 32.0 39.0 14-15 37.26138490251641 39.0 36.0 40.0 32.0 41.0 16-17 37.21183962573018 39.0 36.0 40.0 31.5 41.0 18-19 37.164093778726574 39.0 36.0 40.0 31.0 41.0 20-21 37.11070725838748 39.0 36.0 40.0 31.0 41.0 22-23 36.973892893048244 39.0 36.0 40.0 31.0 41.0 24-25 36.8316498499209 38.5 35.5 40.0 30.5 41.0 26-27 36.544640799592216 38.0 35.0 40.0 30.0 41.0 28-29 36.34976588239167 38.0 35.0 40.0 30.0 41.0 30-31 36.1243513062869 38.0 34.5 40.0 29.5 41.0 32-33 35.90997515021476 38.0 34.0 40.0 29.0 41.0 34-35 36.161843767964 38.0 35.0 40.0 30.0 41.0 36-37 36.24110604516518 38.0 35.0 40.0 30.0 41.0 38-39 36.21236382632428 38.0 35.0 40.0 30.0 41.0 40-41 36.125935665501416 38.0 35.0 40.0 29.5 41.0 42-43 36.001389366723 38.0 35.0 40.0 29.0 41.0 44-45 35.831686388402616 38.0 34.0 40.0 28.5 41.0 46-47 35.643795385949474 38.0 34.0 40.0 28.0 41.0 48-49 35.45522805981747 38.0 34.0 40.0 27.5 41.0 50-51 35.171267621225454 38.0 33.5 40.0 27.0 41.0 52-53 34.938806544657076 38.0 33.0 40.0 26.0 41.0 54-55 34.73079622416395 37.0 33.0 40.0 26.0 41.0 56-57 34.44106053485825 37.0 33.0 40.0 26.0 41.0 58-59 34.16393478206604 37.0 32.5 39.5 26.0 41.0 60-61 33.81546909079596 36.0 32.0 39.0 25.0 41.0 62-63 33.47301687390498 36.0 32.0 39.0 24.5 40.0 64-65 33.13153111794369 35.0 31.0 39.0 23.5 40.0 66-67 32.765270281661046 35.0 31.0 38.0 23.0 40.0 68-69 32.36189846715642 35.0 31.0 38.0 22.5 40.0 70-71 31.93397460177577 34.5 30.5 37.0 22.0 39.5 72-73 31.494778976553512 34.0 30.0 36.5 21.0 39.0 74-75 30.95901232504489 34.0 30.0 36.0 20.0 39.0 76-77 29.61040301221796 32.5 28.0 35.0 18.5 37.0 78-79 30.384818767321864 34.0 29.0 35.0 20.0 37.0 80-81 30.402242739242944 34.0 30.0 35.0 20.0 37.0 82-83 30.147076613960813 34.0 29.0 35.0 18.5 36.0 84-85 29.848576392016668 34.0 29.0 35.0 18.0 36.0 86-87 29.52486244708703 34.0 29.0 35.0 16.0 35.5 88-89 29.194029829148228 33.5 29.0 35.0 11.0 35.0 90-91 28.860001360244468 33.0 29.0 35.0 7.0 35.0 92-93 28.497118343801066 33.0 29.0 35.0 2.0 35.0 94-95 28.06777237177073 33.0 27.0 35.0 2.0 35.0 96-97 27.584017706331178 33.0 27.0 35.0 2.0 35.0 98-99 27.05702987726326 33.0 26.5 35.0 2.0 35.0 100-101 25.78902119919297 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5401665280986307 1101 2 0.6257192698188234 1101 3 0.6266116963864121 1101 4 0.4791875233471359 1101 5 0.5811786377114245 1101 6 0.4807912281281972 1101 7 0.4927488569156111 1101 8 0.5226440383277264 1101 9 0.7783057640504865 1101 10-11 0.7302668161619721 1101 12-13 0.7656428529135653 1101 14-15 0.9390058376281871 1101 16-17 0.8239505623981813 1101 18-19 0.902127386215497 1101 20-21 0.8379486464654136 1101 22-23 0.8938595600547501 1101 24-25 0.7594618021002191 1101 26-27 0.7082524659104692 1101 28-29 0.7142102598547808 1101 30-31 0.8005359976642197 1101 32-33 0.7589124023025775 1101 34-35 0.68260316098538 1101 36-37 0.7813508489824557 1101 38-39 0.8382915139278495 1101 40-41 0.8637699812218997 1101 42-43 0.749702886211935 1101 44-45 0.7779005210683252 1101 46-47 0.6954772404387768 1101 48-49 0.7692511071390058 1101 50-51 0.7769822475796815 1101 52-53 0.7555827271119213 1101 54-55 0.7755590373303036 1101 56-57 0.9440948216269973 1101 58-59 0.8519220640875318 1101 60-61 0.8481067563129798 1101 62-63 1.0206965531307404 1101 64-65 0.992807472643733 1101 66-67 0.8043947985724813 1101 68-69 0.7904182735075125 1101 70-71 0.8022711984791506 1101 72-73 0.8383579746818199 1101 74-75 0.8263520034661376 1101 76-77 0.8607295572176028 1101 78-79 0.8010838721937006 1101 80-81 0.7008991562474272 1101 82-83 0.9647795646710087 1101 84-85 1.0894981310007346 1101 86-87 0.9891326694257039 1101 88-89 0.9840138018799784 1101 90-91 0.9406929586812751 1101 92-93 0.9934133944613066 1101 94-95 0.9288614522290857 1101 96-97 1.0275634041205386 1101 98-99 0.8763821418407183 1101 100-101 1.2515474041447767 1102 1 0.4719086550341629 1102 2 0.5566038910525926 1102 3 0.4415213497067789 1102 4 0.3034340210698119 1102 5 0.35001331261499047 1102 6 0.42070539371716364 1102 7 0.4455185048468735 1102 8 0.49479629612719833 1102 9 0.6377902265921378 1102 10-11 0.6078726076092309 1102 12-13 0.5762396507981649 1102 14-15 0.6934330426836013 1102 16-17 0.7611443831403619 1102 18-19 0.7518771678216964 1102 20-21 0.6909054574757718 1102 22-23 0.7215969234319886 1102 24-25 0.8368152441984478 1102 26-27 0.8138131761264091 1102 28-29 0.8589238955694896 1102 30-31 0.9668476797937089 1102 32-33 0.9296134446540876 1102 34-35 0.8007946716789363 1102 36-37 0.7562769001780154 1102 38-39 0.9094934239158334 1102 40-41 0.8232211053764757 1102 42-43 0.8349200771285155 1102 44-45 1.027102519089219 1102 46-47 0.91193204590585 1102 48-49 0.7893044439004981 1102 50-51 0.8287204433103739 1102 52-53 0.8637465214139652 1102 54-55 0.8115475525296034 1102 56-57 0.8723234924535603 1102 58-59 0.8555754296343849 1102 60-61 0.8793371279445878 1102 62-63 0.6662147700033003 1102 64-65 0.843871110419542 1102 66-67 0.7011879205476603 1102 68-69 0.6483775310407864 1102 70-71 0.607651755643591 1102 72-73 0.6983435610403532 1102 74-75 0.7510951651376381 1102 76-77 0.8250344268112926 1102 78-79 0.8878443754158702 1102 80-81 0.914059190519712 1102 82-83 0.9105508877892632 1102 84-85 0.967132859189828 1102 86-87 1.0618938447721114 1102 88-89 1.0751556887977536 1102 90-91 1.0516236178637506 1102 92-93 1.0138400559484708 1102 94-95 0.9973873249367635 1102 96-97 1.0099442470425863 1102 98-99 0.988653090353349 1102 100-101 1.350783776918945 1103 1 0.3896413555315199 1103 2 0.49705867246084523 1103 3 0.4624852467862155 1103 4 0.2852782131053644 1103 5 0.18512101572390804 1103 6 0.37125940070260555 1103 7 0.4058955432139868 1103 8 0.4165550493365444 1103 9 0.48852907476361906 1103 10-11 0.44374466610086927 1103 12-13 0.5424086742990184 1103 14-15 0.6812351544786566 1103 16-17 0.6831185471593102 1103 18-19 0.6976721054553039 1103 20-21 0.6957560175897939 1103 22-23 0.6514625961668941 1103 24-25 0.7254853358568027 1103 26-27 0.7957156260814244 1103 28-29 0.8689302528573393 1103 30-31 0.7592255809519983 1103 32-33 0.7004789059385743 1103 34-35 0.7410986138631941 1103 36-37 0.8446485390978467 1103 38-39 0.8197312861222485 1103 40-41 0.837018130422905 1103 42-43 0.8661629096825152 1103 44-45 0.8193141986369099 1103 46-47 0.7486249957907276 1103 48-49 0.7911278306841822 1103 50-51 0.9273130467701236 1103 52-53 0.7802681459501102 1103 54-55 0.9122326771418585 1103 56-57 0.9591624028203967 1103 58-59 0.8578164367353125 1103 60-61 0.8318292008497536 1103 62-63 0.824182639641478 1103 64-65 0.7368250245596499 1103 66-67 0.6245326092965513 1103 68-69 0.6957657280666609 1103 70-71 0.6117075399834455 1103 72-73 0.5247632577502017 1103 74-75 0.8321661970960363 1103 76-77 1.0156767241041535 1103 78-79 1.047673226849792 1103 80-81 0.9439120407726342 1103 82-83 0.9736527093163865 1103 84-85 1.043113354323598 1103 86-87 1.0322021651968676 1103 88-89 1.011154399432332 1103 90-91 1.0044498558990647 1103 92-93 1.0744670925362811 1103 94-95 1.039977422674614 1103 96-97 1.2101237388314878 1103 98-99 1.3615611718689742 1103 100-101 1.5059347757064927 1104 1 0.4536705701384207 1104 2 0.530513365868817 1104 3 0.4737413686553822 1104 4 0.28842195013381655 1104 5 0.33362526032008333 1104 6 0.4480489607297429 1104 7 0.4237327683875378 1104 8 0.4477318612444634 1104 9 0.5146089639661824 1104 10-11 0.5432078156303106 1104 12-13 0.5097466796444721 1104 14-15 0.628025626460115 1104 16-17 0.5666584732208548 1104 18-19 0.6018848333311411 1104 20-21 0.6066305216520007 1104 22-23 0.7197268865411601 1104 24-25 0.6170058643493661 1104 26-27 0.7151805393906443 1104 28-29 0.5799439552214949 1104 30-31 0.6954734601571388 1104 32-33 0.8270481438153396 1104 34-35 0.6787830716562482 1104 36-37 0.5911603138310966 1104 38-39 0.5721862972110117 1104 40-41 0.7243025810004866 1104 42-43 0.6861601637980925 1104 44-45 0.7214685153376053 1104 46-47 0.6931240572794977 1104 48-49 0.6893567352266174 1104 50-51 0.7245570406490032 1104 52-53 0.758963490072567 1104 54-55 0.6449538987197059 1104 56-57 0.7433358865961281 1104 58-59 0.745008188659682 1104 60-61 0.7892654868105637 1104 62-63 0.8055782704783567 1104 64-65 0.8552342755845928 1104 66-67 0.8300710782354841 1104 68-69 0.894673487394904 1104 70-71 0.853167488653451 1104 72-73 0.7556710932416593 1104 74-75 0.9744656448124935 1104 76-77 1.0138392864753136 1104 78-79 1.1563130419736076 1104 80-81 0.9503768949551521 1104 82-83 1.1256384146829852 1104 84-85 1.1592046379443985 1104 86-87 1.0282993013596666 1104 88-89 1.0349017900905118 1104 90-91 1.0326998957344315 1104 92-93 0.9595949214736912 1104 94-95 1.095847151503495 1104 96-97 1.0049390279961692 1104 98-99 1.2025884934190572 1104 100-101 1.4822515224948596 1105 1 0.15817297003768616 1105 2 0.26952858704558835 1105 3 0.2686426803573134 1105 4 0.07752108908607624 1105 5 0.1589248542342645 1105 6 0.17906540132374005 1105 7 0.25269624717282113 1105 8 0.21929756977392856 1105 9 0.27759666872803734 1105 10-11 0.32623173840939046 1105 12-13 0.23140210480853085 1105 14-15 0.4006785382329525 1105 16-17 0.29571224852666234 1105 18-19 0.45707771166941313 1105 20-21 0.36188470221521385 1105 22-23 0.5036173245538436 1105 24-25 0.4510556053060384 1105 26-27 0.4602870478644334 1105 28-29 0.3831686179349987 1105 30-31 0.38129591234554283 1105 32-33 0.39828845350756126 1105 34-35 0.2856543883452005 1105 36-37 0.4355106510761715 1105 38-39 0.27648126363561687 1105 40-41 0.3172117360735811 1105 42-43 0.31463322478386146 1105 44-45 0.24831855849767948 1105 46-47 0.2369740358237209 1105 48-49 0.29122032286477406 1105 50-51 0.3136381202971634 1105 52-53 0.2998530681972724 1105 54-55 0.2697249680458924 1105 56-57 0.3476490780065262 1105 58-59 0.22552750134576627 1105 60-61 0.2801155498524537 1105 62-63 0.1740511989187823 1105 64-65 0.10562851516363025 1105 66-67 0.17449332133359974 1105 68-69 0.17558563136565652 1105 70-71 0.11749738077810079 1105 72-73 0.16706850231140535 1105 74-75 0.3687484667692935 1105 76-77 0.34676852216911414 1105 78-79 0.3592873596549886 1105 80-81 0.43727975743768965 1105 82-83 0.5045262597772329 1105 84-85 0.6326281352528973 1105 86-87 0.6169774431401152 1105 88-89 0.6313694564804919 1105 90-91 0.6587415779337569 1105 92-93 0.6643155241574057 1105 94-95 0.5126689790719361 1105 96-97 0.4408523565910052 1105 98-99 0.5834300569902915 1105 100-101 1.0268358022848396 1106 1 0.04395784919060475 1106 2 0.07125779195235893 1106 3 0.11429409110365896 1106 4 6.256337655585753E-4 1106 5 0.06311780835951453 1106 6 0.08614270400481416 1106 7 0.061064435419446284 1106 8 0.14388357364558146 1106 9 0.12972591108085396 1106 10-11 0.1337961740272675 1106 12-13 0.062397066309316074 1106 14-15 0.19431638816124064 1106 16-17 0.11701670828386312 1106 18-19 0.1554809052821895 1106 20-21 0.09932021644638667 1106 22-23 0.07283767781716222 1106 24-25 0.15337575562517003 1106 26-27 0.3181368424126916 1106 28-29 0.06339643700373188 1106 30-31 0.130927971510296 1106 32-33 0.2738913515640391 1106 34-35 0.2527829453016395 1106 36-37 0.2490418800754597 1106 38-39 0.22535671206873786 1106 40-41 0.1913373541840926 1106 42-43 0.19148137103741192 1106 44-45 0.18059872178918823 1106 46-47 0.2424842883504894 1106 48-49 0.2998454511676343 1106 50-51 0.3501633947064704 1106 52-53 0.14736374199206637 1106 54-55 0.2778646351104186 1106 56-57 0.3313759291618368 1106 58-59 0.3500720470951393 1106 60-61 0.04178517542542437 1106 62-63 -0.0023158325124938983 1106 64-65 0.14127347315385208 1106 66-67 0.05596103916427353 1106 68-69 0.07787385224383314 1106 70-71 0.04539184310971578 1106 72-73 0.020634736676843346 1106 74-75 0.2824935060040552 1106 76-77 0.1018225681809426 1106 78-79 0.29769496655174876 1106 80-81 0.28951414962483923 1106 82-83 0.33796006218915764 1106 84-85 0.27880964158696386 1106 86-87 0.38288883643393135 1106 88-89 0.5398315941816385 1106 90-91 0.44510653645891907 1106 92-93 0.5599945179286543 1106 94-95 0.6418264096045014 1106 96-97 0.6218575154525148 1106 98-99 0.6204259145214621 1106 100-101 0.9792940348983414 1107 1 0.05278896956803791 1107 2 0.14838097761509772 1107 3 0.2135355034640014 1107 4 0.08959280260508251 1107 5 0.17728732934592273 1107 6 0.1596599342021321 1107 7 0.11869728632425591 1107 8 0.022204560234470705 1107 9 0.09844476772284594 1107 10-11 0.24427692668288614 1107 12-13 0.08323430873678461 1107 14-15 0.05768921669350391 1107 16-17 0.10282160248062411 1107 18-19 0.2909132832383676 1107 20-21 0.2758993106872083 1107 22-23 0.3241169731407041 1107 24-25 0.2184172524982202 1107 26-27 0.29250299165150295 1107 28-29 0.2961349124493893 1107 30-31 0.27018412456067153 1107 32-33 0.4196684729408915 1107 34-35 0.33084513078981814 1107 36-37 0.24854843525857717 1107 38-39 0.31403577930820603 1107 40-41 0.2974773506405768 1107 42-43 0.3704608397667357 1107 44-45 0.40834107527416563 1107 46-47 0.45951610527129816 1107 48-49 0.36317991337107713 1107 50-51 0.3323325861848474 1107 52-53 0.20398268011756215 1107 54-55 0.2731690911617477 1107 56-57 0.2613270870055544 1107 58-59 0.3032637094086823 1107 60-61 0.3223357676572718 1107 62-63 0.20709703734774365 1107 64-65 0.2841120453815975 1107 66-67 0.3718332253519989 1107 68-69 0.4824255960103727 1107 70-71 0.32420352861522517 1107 72-73 0.39658150020841276 1107 74-75 0.5030940126008652 1107 76-77 0.36997890753847784 1107 78-79 0.5084696177079984 1107 80-81 0.5407381455678824 1107 82-83 0.48021039066203386 1107 84-85 0.5319023752156795 1107 86-87 0.3537845817922154 1107 88-89 0.46597286987896425 1107 90-91 0.5857031682853382 1107 92-93 0.507622681189428 1107 94-95 0.6699118480735642 1107 96-97 0.7217065127303677 1107 98-99 0.695681070766458 1107 100-101 0.9571008407647206 1108 1 0.14843358240885252 1108 2 0.22352982446391323 1108 3 0.2913374018795061 1108 4 0.12253796525872929 1108 5 0.15115594629784823 1108 6 0.05659519242137634 1108 7 0.10944914747680912 1108 8 0.2594911972372316 1108 9 0.2244483780588098 1108 10-11 0.19644265284141227 1108 12-13 0.3210099706308043 1108 14-15 0.319225728639438 1108 16-17 0.4359174453482808 1108 18-19 0.39511574970745755 1108 20-21 0.3444350918007828 1108 22-23 0.41022896131623554 1108 24-25 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-0.9332988517068195 2316 86-87 -0.8525578572644825 2316 88-89 -1.0191497068809205 2316 90-91 -0.5682791814849715 2316 92-93 -0.7813974644617012 2316 94-95 -0.5798888966846469 2316 96-97 -0.5876412157737896 2316 98-99 -0.49715667730423263 2316 100-101 -0.7082744701193775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 54.0 5 97.0 6 205.0 7 624.0 8 1406.0 9 2185.0 10 3237.0 11 4636.0 12 6162.0 13 7893.0 14 9456.0 15 11304.0 16 13145.0 17 14798.0 18 15086.0 19 15311.0 20 16524.0 21 17847.0 22 19985.0 23 23552.0 24 28851.0 25 36412.0 26 46819.0 27 59639.0 28 74596.0 29 91700.0 30 111016.0 31 132713.0 32 157649.0 33 189200.0 34 230852.0 35 285580.0 36 365816.0 37 439113.0 38 300074.0 39 30648.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.59516851997389 19.691950090241082 13.6817275920611 25.031153797723928 2 16.497180864245546 20.828429767613493 37.963457054532235 24.71093231360873 3 19.446384983591543 24.62053967931239 29.421845554629805 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25.54315738902427 26.47666166945469 23.825052239194736 24-25 24.07581592470864 25.689486450847355 26.47593603200932 23.758761592434688 26-27 23.940439134581016 25.888279421915634 26.298240625375342 23.873040818128015 28-29 23.904668568838318 26.047487726145164 26.288587440385296 23.75925626463122 30-31 24.263434426755882 25.76839412777188 26.237944448816087 23.73022699665615 32-33 24.097290823102004 25.89122041417442 26.030532970035136 23.980955792688444 34-35 24.125487639236333 25.88444424504292 26.254004412723518 23.736063702997225 36-37 24.251349536148105 25.67209097925776 26.203367676721268 23.873191807872864 38-39 24.13720188141949 25.718515318866526 26.224812010898674 23.91947078881531 40-41 24.19006456040552 25.6321867564976 26.327570934413785 23.850177748683095 42-43 24.154289970077404 25.743944974691246 26.046933670819577 24.054831384411777 44-45 23.982508471068662 25.703459141056882 26.234706534974283 24.079325852900173 46-47 24.16407152373708 25.729225364533537 26.0109932153934 24.095709896335986 48-49 24.12347381727849 25.72805961962747 26.009308511600544 24.139158051493496 50-51 24.121328665182816 25.78081014675826 26.04309009046067 24.054771097598255 52-53 24.222986050748933 25.6705020365845 26.006587794826082 24.099924117840487 54-55 24.005680122069435 25.60053233504832 26.14440999351226 24.24937754936999 56-57 24.03205335085452 25.704549960707585 25.974875284562494 24.288521403875404 58-59 23.998114788058395 25.693050587726585 26.13003258413346 24.17880204008156 60-61 24.08338645067905 25.829616928923382 26.102623797801538 23.98437282259603 62-63 24.070096957161564 25.71805367261098 26.00455093220241 24.207298438025052 64-65 23.828230807225616 25.6840646487354 25.986091657167027 24.501612886871957 66-67 23.903047764522427 25.759587252992233 25.946738630137382 24.390626352347958 68-69 23.97723153516032 25.653831989052133 26.01393253039797 24.35500394538958 70-71 23.84785249238558 25.528718436804265 26.011862614406372 24.61156645640378 72-73 23.870295208328844 25.65141737683785 25.962932747976463 24.515354666856844 74-75 23.877267722769332 25.7414283437767 25.91749984272856 24.463804090725407 76-77 23.813319722975532 25.594564417765437 25.920349661409702 24.671766197849333 78-79 23.83146065179918 25.638898471433833 25.88620350503465 24.643437371732336 80-81 23.883672061806273 25.73305952151185 25.79059907520678 24.592669341475094 82-83 23.967815024117208 25.56168871456536 25.98651187374037 24.48398438757706 84-85 23.900761483664947 25.626897242574636 25.738791820734253 24.733549453026164 86-87 23.76482747867473 25.696700213334083 25.85490098614106 24.68357132185012 88-89 23.75718379889198 25.475489163976928 25.831269449527277 24.93605758760382 90-91 23.73301312206529 25.57059791965184 25.815333057043784 24.881055901239087 92-93 23.82626485503949 25.34640123907859 25.973376310057674 24.853957595824244 94-95 23.590829188314284 25.312400399253153 25.99539245362336 25.101377958809202 96-97 23.637232206392497 25.213053716271094 25.9677904239513 25.18192365338511 98-99 23.48038806038183 25.30570228228039 25.88027895141069 25.333630705927085 100-101 23.455914512976406 25.28124682323225 25.873749054431123 25.389089609360216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 138.0 1 111.0 2 108.0 3 135.0 4 117.5 5 118.5 6 173.5 7 324.5 8 526.5 9 608.5 10 538.5 11 459.5 12 492.0 13 563.5 14 607.5 15 670.5 16 780.5 17 853.0 18 931.5 19 1086.5 20 1227.0 21 1389.0 22 1624.5 23 1952.5 24 2359.5 25 2918.5 26 3721.5 27 4692.0 28 5939.0 29 7654.5 30 9743.0 31 12835.0 32 17769.0 33 22505.5 34 27478.0 35 34398.0 36 43280.0 37 53322.0 38 62228.5 39 69391.5 40 76139.5 41 82750.0 42 88669.0 43 94701.5 44 101055.5 45 108054.0 46 114585.0 47 119607.0 48 123903.0 49 125829.0 50 124214.5 51 123099.0 52 123206.0 53 122543.0 54 120097.5 55 112421.5 56 101728.0 57 90227.5 58 79700.5 59 69546.0 60 58020.5 61 47777.0 62 38653.5 63 29646.0 64 21943.5 65 16545.0 66 12371.0 67 9522.5 68 7572.5 69 5843.5 70 4546.0 71 3513.5 72 2699.0 73 2117.5 74 1661.5 75 1307.0 76 939.0 77 632.5 78 409.0 79 265.5 80 176.5 81 107.5 82 64.0 83 41.0 84 21.0 85 11.0 86 10.0 87 5.0 88 2.0 89 1.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01812453399869547 2 0.002315309732368283 3 0.003111197452869881 4 0.03252286639686073 5 0.0020620727303905023 6 1.4470685827301769E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.617671456825442E-5 24-25 5.064740039555619E-4 26-27 0.0016279521555714492 28-29 0.0 30-31 0.006566073694138179 32-33 0.023116920609114577 34-35 0.013855681679641445 36-37 6.692692195127068E-4 38-39 7.054459340809613E-4 40-41 0.0019354542294016117 42-43 0.008845206711938208 44-45 0.0087728532828017 46-47 0.0083749094225509 48-49 0.01038271708108902 50-51 0.17883958846816575 52-53 0.049652540744929204 54-55 0.019390719008584374 56-57 0.011468018518136653 58-59 0.02148896845354313 60-61 0.052745649840514955 62-63 0.09322739344239166 64-65 0.09771330604885521 66-67 0.10158421450765844 68-69 0.09977537877924571 70-71 0.09071311177989796 72-73 0.06893472960980881 74-75 0.22568843383405524 76-77 0.2707827085433844 78-79 0.007723728560322321 80-81 8.50152792353979E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0853014370476328E-4 90-91 0.007253431270935012 92-93 0.019625867653278027 94-95 0.0017364822992762124 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2764209.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.35236063344021 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26901839012478 42.97939229478796 2 12.895782968528598 14.534156249861995 3 4.299410087614406 7.2684572330489345 4 1.9926286175407277 4.49157305856674 5 1.1436194331866578 3.222282736317251 6 0.7268645441263274 2.457631975336075 7 0.48652567800338353 1.9191809324992974 8 0.36588012201818143 1.6494566867660536 9 0.2732473757234845 1.3858321193019842 >10 1.4460713940337064 14.452527435421828 >50 0.07642253402932643 2.912151670777958 >100 0.023498090147177724 2.2197582542453924 >500 7.730736924794172E-4 0.280809683601319 >1k 2.576912308032055E-4 0.22678966946711826 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.617671456825443E-5 2 0.0 0.0 0.0 0.0 3.617671456825443E-5 3 0.0 0.0 0.0 0.0 3.617671456825443E-5 4 0.0 0.0 0.0 0.0 3.617671456825443E-5 5 0.0 0.0 0.0 0.0 7.235342913650886E-5 6 0.0 0.0 0.0 0.0 1.447068582730177E-4 7 0.0 0.0 0.0 0.0 1.447068582730177E-4 8 0.0 0.0 0.0 0.0 1.447068582730177E-4 9 0.0 0.0 0.0 3.617671456825443E-5 1.447068582730177E-4 10-11 0.0 0.0 0.0 3.617671456825443E-5 1.8088357284127212E-4 12-13 0.0 0.0 0.0 3.617671456825443E-5 6.150041476603253E-4 14-15 0.0 0.0 0.0 3.617671456825443E-5 7.59711005933343E-4 16-17 0.0 0.0 0.0 5.426507185238164E-5 9.044178642063607E-4 18-19 0.0 0.0 0.0 1.0853014370476328E-4 9.767712933428695E-4 20-21 0.0 0.0 0.0 1.266185009888905E-4 0.001175743223468269 22-23 0.0 0.0 0.0 2.3514864469365376E-4 0.0012480966526047777 24-25 0.0 0.0 0.0 2.8941371654603537E-4 0.001266185009888905 26-27 0.0 0.0 0.0 6.87357576796834E-4 0.0014108918681619226 28-29 0.0 0.0 0.0 0.00220677958866352 0.0014832452972984315 30-31 0.0 0.0 0.0 0.0081759374924255 0.001519422011866686 32-33 0.0 0.0 0.0 0.02445545904813999 0.001609863798287322 34-35 0.0 0.0 0.0 0.0479884118747895 0.0016279521555714492 36-37 0.0 0.0 0.0 0.08293511814772328 0.0016279521555714492 38-39 0.0 0.0 0.0 0.1354094426289763 0.0018450124429809757 40-41 0.0 0.0 0.0 0.19390719008584373 0.0019173658721174847 42-43 0.0 0.0 0.0 0.2535445040516111 0.0019173658721174847 44-45 0.0 0.0 0.0 0.3277610339883851 0.0020258960158222477 46-47 0.0 0.0 0.0 0.4165748682534497 0.0021886912313793926 48-49 0.0 0.0 0.0 0.5082647513266906 0.0023153097323682834 50-51 0.0 0.0 0.0 0.6093967569022458 0.002387663161504792 52-53 0.0 0.0 0.0 0.7080144808153074 0.0025866350916301916 54-55 0.0 1.8088357284127214E-5 0.0 0.8171958053822992 0.0026409001634825732 56-57 0.0 3.617671456825443E-5 0.0 0.9434525392255072 0.002713253592619082 58-59 0.0 3.617671456825443E-5 0.0 1.083347894460947 0.0027856070217555907 60-61 0.0 3.617671456825443E-5 0.0 1.241331606980514 0.0027856070217555907 62-63 0.0 3.617671456825443E-5 0.0 1.4029329909569066 0.003038844023733372 64-65 0.0 5.426507185238164E-5 0.0 1.57063015133805 0.003111197452869881 66-67 3.617671456825443E-5 7.235342913650886E-5 0.0 1.7648267551404397 0.003165462524722262 68-69 3.617671456825443E-5 7.235342913650886E-5 0.0 1.9839852919949252 0.0031835508820063896 70-71 3.617671456825443E-5 7.235342913650886E-5 0.0 2.2233847006503487 0.0033463460975635346 72-73 3.617671456825443E-5 7.235342913650886E-5 0.0 2.473799918891806 0.003581494742257188 74-75 3.617671456825443E-5 7.235342913650886E-5 0.0 2.730039588178752 0.0036719365286778244 76-77 3.617671456825443E-5 7.235342913650886E-5 0.0 3.015238717477586 0.0037081132432460785 78-79 3.617671456825443E-5 7.235342913650886E-5 0.0 3.3311518774448676 0.003744289957814333 80-81 3.617671456825443E-5 7.235342913650886E-5 0.0 3.680600851816921 0.003834731744234969 82-83 3.617671456825443E-5 7.235342913650886E-5 0.0 4.059208258130988 0.00408796874621275 84-85 3.617671456825443E-5 7.235342913650886E-5 0.0 4.448288823312565 0.004178410532633386 86-87 3.617671456825443E-5 7.235342913650886E-5 0.0 4.85594613142494 0.004395470820042912 88-89 3.617671456825443E-5 7.235342913650886E-5 0.0 5.300648395255207 0.004612531107452439 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2075 0.0 47.828217 1 GTATCAA 3980 0.0 38.894707 1 CAACGCA 4525 0.0 33.142765 5 TCAACGC 4680 0.0 32.15871 4 ATCAACG 4730 0.0 31.71897 3 AACGCAG 4930 0.0 30.515245 6 TATCAAC 5085 0.0 29.878584 2 GTACATG 8620 0.0 27.929083 1 TACATGG 9000 0.0 26.007906 2 ACGCAGA 5905 0.0 25.235638 7 ACATGGG 8835 0.0 25.203419 3 CGCAGAG 6240 0.0 23.804787 8 CATGGGG 6955 0.0 22.253878 4 CTAGTAC 870 0.0 20.73755 3 GAGTACA 6090 0.0 20.428665 1 GTACTAA 450 0.0 18.99398 1 GCAGAGT 7740 0.0 18.884882 9 AGTACAT 6365 0.0 18.723038 2 ATGGGGG 4190 0.0 18.575907 5 TATACTG 1080 0.0 18.456348 5 >>END_MODULE