FastQCFastQC Report
Sat 18 Jun 2016
SRR1930970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933873
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54430.18552268622397766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.050.458421
GTATCAA40950.044.3811721
ATCAACG52700.034.9468573
TATCAAC54000.033.9253582
TCAACGC54350.033.785634
CAACGCA54500.032.3649025
GTACATG81550.030.2494581
AACGCAG60700.029.6202646
TACATGG83250.029.0788822
ACATGGG90150.027.629763
ACGCAGA70400.024.5801587
CGCAGAG73050.024.187058
CATGGGG71000.024.091254
GCAGAGT74450.021.4112249
AGAGTAC66450.018.94902210-11
ATGGGGG42900.018.8120925
GAGTACA58550.018.4006181
AGTACAT58250.018.1442992
CAGAGTA71100.018.02098510-11
GTGGTAT11300.017.3752731
Produced by FastQC (version 0.11.5)