##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930970_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933873 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.668872851687855 28.0 10.0 31.0 2.0 33.0 2 20.725448579403402 27.0 2.0 31.0 2.0 33.0 3 21.11762301912864 27.0 10.0 31.0 2.0 33.0 4 22.993926117456347 32.0 2.0 35.0 2.0 37.0 5 22.96217389096256 30.0 2.0 35.0 2.0 37.0 6 23.55421076508765 31.0 10.0 35.0 2.0 37.0 7 23.54680042387656 31.0 10.0 35.0 2.0 37.0 8 24.128778239548883 32.0 10.0 35.0 2.0 37.0 9 25.094284926443645 32.0 10.0 37.0 2.0 39.0 10-11 25.17125247071022 32.0 10.0 37.0 2.0 39.0 12-13 24.98841446102132 32.0 10.0 37.0 2.0 39.0 14-15 25.124778748091686 32.0 10.0 38.0 2.0 40.0 16-17 24.299131387077765 31.5 9.0 37.0 2.0 39.0 18-19 23.711659298135945 30.5 2.0 37.0 2.0 39.0 20-21 23.1864042853934 30.0 2.0 37.0 2.0 39.0 22-23 23.02946923742098 29.5 2.0 37.0 2.0 39.0 24-25 22.672278759169195 29.0 2.0 37.0 2.0 39.0 26-27 22.25904342144326 28.0 2.0 36.0 2.0 39.0 28-29 22.071844111861694 27.0 2.0 36.0 2.0 39.0 30-31 21.886756345622324 27.0 2.0 36.0 2.0 39.0 32-33 21.47426609808945 27.0 2.0 36.0 2.0 39.0 34-35 18.870179793058526 18.5 2.0 34.0 2.0 38.0 36-37 17.36403092431063 16.0 2.0 32.5 2.0 38.0 38-39 16.47609388681787 13.5 2.0 31.5 2.0 38.0 40-41 17.434459160297667 15.0 2.0 33.0 2.0 38.0 42-43 17.272490833788645 12.0 2.0 33.0 2.0 38.0 44-45 15.583217133120622 8.5 2.0 31.5 2.0 37.5 46-47 16.15817709219179 13.0 2.0 30.5 2.0 37.0 48-49 15.553289286891424 7.5 2.0 31.0 2.0 37.0 50-51 13.974312794043914 7.0 2.0 28.5 2.0 36.0 52-53 16.36197851781587 13.0 2.0 31.0 2.0 37.0 54-55 17.599502432450212 16.0 2.0 33.5 2.0 38.0 56-57 17.699683830895204 16.0 2.0 34.0 2.0 38.0 58-59 17.336846380194373 12.0 2.0 34.0 2.0 38.0 60-61 16.961262126888247 8.5 2.0 33.0 2.0 38.0 62-63 16.46795566815605 7.5 2.0 32.5 2.0 37.0 64-65 15.95915041312286 4.0 2.0 32.0 2.0 36.5 66-67 15.50694150701138 2.0 2.0 31.5 2.0 36.0 68-69 15.034676177189674 2.0 2.0 31.0 2.0 35.5 70-71 14.482123288908554 2.0 2.0 30.5 2.0 35.0 72-73 13.989523915997728 2.0 2.0 30.0 2.0 34.5 74-75 13.469486409261751 2.0 2.0 29.5 2.0 34.0 76-77 12.928957217984555 2.0 2.0 29.0 2.0 34.0 78-79 12.437383963109514 2.0 2.0 28.5 2.0 34.0 80-81 11.941280859805453 2.0 2.0 27.0 2.0 34.0 82-83 11.475131166209309 2.0 2.0 26.0 2.0 33.0 84-85 10.981567027611625 2.0 2.0 25.5 2.0 33.0 86-87 10.465816175410456 2.0 2.0 24.5 2.0 32.0 88-89 9.965490837537956 2.0 2.0 22.5 2.0 32.0 90-91 9.455235110722242 2.0 2.0 19.5 2.0 32.0 92-93 8.899079646596835 2.0 2.0 14.0 2.0 31.0 94-95 8.334917189667038 2.0 2.0 4.5 2.0 31.0 96-97 7.72425340156169 2.0 2.0 2.0 2.0 31.0 98-99 7.079807476329071 2.0 2.0 2.0 2.0 30.5 100-101 6.312308508241495 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.41089122235401 1101 2 8.492428180345229 1101 3 8.00050004348088 1101 4 9.966995508039584 1101 5 9.692912133183395 1101 6 9.502638600879425 1101 7 9.071780783715884 1101 8 8.374173449204488 1101 9 8.738598817481183 1101 10-11 8.254842323381393 1101 12-13 8.264193150760534 1101 14-15 8.625551083497935 1101 16-17 9.328553455155902 1101 18-19 9.706950055736442 1101 20-21 10.052809544331495 1101 22-23 9.910676399321357 1101 24-25 10.055748795148315 1101 26-27 10.04467695066204 1101 28-29 9.882345043662951 1101 30-31 9.817301560784912 1101 32-33 9.790289646091441 1101 34-35 12.063996854862758 1101 36-37 6.704030683512723 1101 38-39 9.338334164479402 1101 40-41 11.001438917099232 1101 42-43 11.204275953587963 1101 44-45 5.7263277590226025 1101 46-47 7.326984127620415 1101 48-49 10.1069149331641 1101 50-51 -1.514768757542921 1101 52-53 7.5161230411069155 1101 54-55 9.920656010794612 1101 56-57 10.263722585985132 1101 58-59 10.628383818106471 1101 60-61 10.249182078274885 1101 62-63 10.4070170033939 1101 64-65 10.33851353445506 1101 66-67 10.211653510810425 1101 68-69 10.145898677090996 1101 70-71 9.975035657320726 1101 72-73 9.798328304571216 1101 74-75 9.915685647798549 1101 76-77 9.730720182309414 1101 78-79 9.605837631119472 1101 80-81 9.523996793074451 1101 82-83 9.051014592752232 1101 84-85 8.875722151858321 1101 86-87 8.525385537084508 1101 88-89 8.420517101931928 1101 90-91 7.997887601791312 1101 92-93 7.760929933649896 1101 94-95 7.181313642777495 1101 96-97 6.9971909755993416 1101 98-99 6.533023041381592 1101 100-101 5.784174398767654 1102 1 6.899183337184219 1102 2 7.745085381774352 1102 3 7.617014361946037 1102 4 9.234208640521501 1102 5 9.102137263872393 1102 6 8.508431356271675 1102 7 8.410744037634238 1102 8 7.719198704232046 1102 9 7.575998791042139 1102 10-11 7.474169774063629 1102 12-13 7.1675156760117 1102 14-15 7.4544811275019605 1102 16-17 8.192612065093233 1102 18-19 8.368007988366301 1102 20-21 8.919535783452144 1102 22-23 8.580199240737617 1102 24-25 8.726511104808694 1102 26-27 8.759848030935448 1102 28-29 8.658555562475172 1102 30-31 8.319996942139468 1102 32-33 8.465499254985556 1102 34-35 10.248966345129855 1102 36-37 1.9102844890912714 1102 38-39 4.0092472076026375 1102 40-41 8.352899475801753 1102 42-43 9.372036283850939 1102 44-45 1.9173979820573663 1102 46-47 2.66978165020671 1102 48-49 7.597083547190872 1102 50-51 -3.018170633037963 1102 52-53 5.4743854859418875 1102 54-55 7.9045654343285 1102 56-57 8.562395701160288 1102 58-59 8.594573342373568 1102 60-61 8.454571042334333 1102 62-63 8.44730515589821 1102 64-65 8.282847699728233 1102 66-67 8.073527092442745 1102 68-69 8.050696370935222 1102 70-71 8.078465130501757 1102 72-73 8.084162371525567 1102 74-75 7.893546201469258 1102 76-77 7.652844500119118 1102 78-79 7.702537671749093 1102 80-81 7.500272438453122 1102 82-83 7.294303257044092 1102 84-85 7.009742076482764 1102 86-87 6.877667074530519 1102 88-89 6.831935481748033 1102 90-91 6.7781982694169685 1102 92-93 6.496117557086368 1102 94-95 5.837445647773107 1102 96-97 5.724304648272026 1102 98-99 5.233204859483158 1102 100-101 4.866975898245602 1103 1 7.025285459229881 1103 2 7.768089943901401 1103 3 7.405719542352475 1103 4 9.123082115919793 1103 5 9.235443861937654 1103 6 8.633086594355301 1103 7 8.32150751283 1103 8 7.797789177054298 1103 9 8.014518853490728 1103 10-11 7.381734163557656 1103 12-13 7.365412609266663 1103 14-15 7.724694492798559 1103 16-17 8.297656406947223 1103 18-19 8.289419366614744 1103 20-21 8.820948193944709 1103 22-23 8.768345876895733 1103 24-25 8.632139111029165 1103 26-27 8.696453269639722 1103 28-29 8.597273635044544 1103 30-31 8.305423190365438 1103 32-33 8.147754967553041 1103 34-35 10.31340287812236 1103 36-37 -2.121931109685452 1103 38-39 -1.1034343797100803 1103 40-41 6.2713362923347304 1103 42-43 8.710568530700147 1103 44-45 1.4223482895202082 1103 46-47 1.7118471772381145 1103 48-49 7.253008759143912 1103 50-51 -3.497061458412963 1103 52-53 4.731069375551002 1103 54-55 7.6250416189974075 1103 56-57 7.985550733557286 1103 58-59 8.378925730047872 1103 60-61 8.312928909613436 1103 62-63 8.24587855749322 1103 64-65 8.077938475146105 1103 66-67 7.765512576999697 1103 68-69 7.892499078594318 1103 70-71 7.8519414978822315 1103 72-73 7.612267232284763 1103 74-75 7.288311362663764 1103 76-77 7.223466984704595 1103 78-79 6.965645702194633 1103 80-81 6.69596333098932 1103 82-83 6.479303399685421 1103 84-85 6.287947221718861 1103 86-87 6.193516246677261 1103 88-89 6.049456214284216 1103 90-91 5.935251763400007 1103 92-93 5.528379487073648 1103 94-95 5.301502264839064 1103 96-97 4.822057023794599 1103 98-99 4.479214931049905 1103 100-101 3.9881983257309983 1104 1 6.647556917771393 1104 2 7.514685132087312 1104 3 6.94510615774843 1104 4 8.597049188741458 1104 5 8.547532507880074 1104 6 7.8270488166270376 1104 7 7.668259165530625 1104 8 7.096056433785506 1104 9 7.397084554446614 1104 10-11 6.80181749703382 1104 12-13 6.504851136687698 1104 14-15 7.053676935693193 1104 16-17 7.499014218132281 1104 18-19 8.067353199457592 1104 20-21 8.228560268643179 1104 22-23 8.204209084103343 1104 24-25 7.875352358258095 1104 26-27 8.056040133047894 1104 28-29 7.859932145617201 1104 30-31 7.561044493535093 1104 32-33 7.391223543530991 1104 34-35 9.44041535286265 1104 36-37 -2.4752533400300116 1104 38-39 -1.7489712310347265 1104 40-41 5.305798335496053 1104 42-43 7.30318591443692 1104 44-45 0.5241933410165949 1104 46-47 0.3335725739805344 1104 48-49 5.7254169714848775 1104 50-51 -4.014136776055425 1104 52-53 3.568723354801616 1104 54-55 5.951845760047288 1104 56-57 6.407011781755987 1104 58-59 6.529701507378494 1104 60-61 6.544957664976035 1104 62-63 6.433124032170095 1104 64-65 6.577904463449933 1104 66-67 6.278490261899062 1104 68-69 6.205521948165947 1104 70-71 6.413451034440465 1104 72-73 6.206937636988593 1104 74-75 6.120364981379685 1104 76-77 5.9086284261749125 1104 78-79 5.589653846147987 1104 80-81 5.449189139514294 1104 82-83 5.340767601598561 1104 84-85 4.956510044910315 1104 86-87 4.870043966370323 1104 88-89 4.759002435054512 1104 90-91 4.312014426249078 1104 92-93 4.059181228986683 1104 94-95 3.8837073561168154 1104 96-97 3.65224567676432 1104 98-99 3.0419932377794874 1104 100-101 2.574008067623467 1105 1 6.074524478319322 1105 2 6.976011253723723 1105 3 6.628398785205615 1105 4 8.405543697568206 1105 5 8.301681376025996 1105 6 7.481538328683886 1105 7 7.168606071884035 1105 8 6.626463888069676 1105 9 6.905568516001807 1105 10-11 6.458815282106794 1105 12-13 6.152721411839643 1105 14-15 6.280909661240003 1105 16-17 6.8618296474721525 1105 18-19 7.512659828417764 1105 20-21 7.744900711496744 1105 22-23 7.360217650465458 1105 24-25 7.420518164662926 1105 26-27 7.26870279175137 1105 28-29 7.293862141398293 1105 30-31 6.993512291396634 1105 32-33 6.79062317305808 1105 34-35 8.373194212834438 1105 36-37 -3.102064916319746 1105 38-39 -1.9625411198928528 1105 40-41 4.7965793609545315 1105 42-43 6.832731870876689 1105 44-45 -2.7158773916232537 1105 46-47 -3.5648534929419036 1105 48-49 3.9665308952017373 1105 50-51 -7.997627412055899 1105 52-53 0.5193118595940334 1105 54-55 4.8302344830363815 1105 56-57 5.752812319666834 1105 58-59 6.043273291449477 1105 60-61 6.1504048363296455 1105 62-63 6.212344094398997 1105 64-65 6.095553427180505 1105 66-67 5.784278901169195 1105 68-69 5.785841069050859 1105 70-71 5.918218730784519 1105 72-73 5.589243682552807 1105 74-75 5.410334608533637 1105 76-77 5.466688272052908 1105 78-79 5.278866953769706 1105 80-81 4.997180873090214 1105 82-83 5.023839395557609 1105 84-85 4.737360814201935 1105 86-87 4.555369003615374 1105 88-89 4.492062528134184 1105 90-91 4.091665031398785 1105 92-93 3.838697109222414 1105 94-95 3.6014644293807994 1105 96-97 3.4560672498898715 1105 98-99 3.0997766297820197 1105 100-101 2.9942814067578 1106 1 5.894968291188658 1106 2 6.784794785375929 1106 3 6.613048373609509 1106 4 7.675819912492464 1106 5 7.7543872303734105 1106 6 6.8557779454925765 1106 7 6.872944076085243 1106 8 6.232579363375521 1106 9 6.191034299964517 1106 10-11 5.913846115625095 1106 12-13 5.9300515781830185 1106 14-15 6.131098691455886 1106 16-17 6.517450250438731 1106 18-19 6.7838476185375285 1106 20-21 7.250494815651951 1106 22-23 6.706575612011218 1106 24-25 6.628061962091376 1106 26-27 6.750586290270011 1106 28-29 6.709043628098222 1106 30-31 6.4864868895315055 1106 32-33 6.31908466574135 1106 34-35 8.127869704932241 1106 36-37 -0.030026148438075495 1106 38-39 1.166815501834403 1106 40-41 5.8323085246053985 1106 42-43 6.800001817766475 1106 44-45 -2.9019143275081944 1106 46-47 -3.637291150366556 1106 48-49 3.7541821274165716 1106 50-51 -8.063470725669934 1106 52-53 -0.06007258789569647 1106 54-55 3.7456855820735804 1106 56-57 4.797616823580135 1106 58-59 5.291344186023615 1106 60-61 5.295578313070205 1106 62-63 5.276687551162908 1106 64-65 5.065212087104651 1106 66-67 5.133664321756395 1106 68-69 4.958545765331861 1106 70-71 4.867516934494832 1106 72-73 4.800511186244146 1106 74-75 4.685881634072219 1106 76-77 4.527392263843504 1106 78-79 4.383209340930611 1106 80-81 4.156619476269105 1106 82-83 3.8095227190795473 1106 84-85 3.5897929949374987 1106 86-87 3.5470029790321984 1106 88-89 3.4884944370458264 1106 90-91 3.2172024465945466 1106 92-93 3.0229400474299215 1106 94-95 2.7417179333359183 1106 96-97 2.5137023656804285 1106 98-99 2.3066615758836795 1106 100-101 2.1980361556343144 1107 1 5.883265991558787 1107 2 7.063156284696952 1107 3 6.325002941829666 1107 4 7.732703669556798 1107 5 8.073171976136795 1107 6 6.547163081365714 1107 7 6.73204453192821 1107 8 6.216131264614443 1107 9 6.5050239963326675 1107 10-11 6.055649789377046 1107 12-13 5.5276025492274385 1107 14-15 5.80700022945425 1107 16-17 6.5739166710923875 1107 18-19 6.675069324955906 1107 20-21 7.150960876063753 1107 22-23 7.053678018402952 1107 24-25 7.068830675305101 1107 26-27 6.99538353606815 1107 28-29 6.93737038413823 1107 30-31 6.847291642817467 1107 32-33 6.42415340163403 1107 34-35 8.443755586112804 1107 36-37 0.1635303680272031 1107 38-39 1.1388897581256288 1107 40-41 5.860533741693811 1107 42-43 6.780968477323629 1107 44-45 -2.82587132408411 1107 46-47 -3.678292342699562 1107 48-49 3.493251150470064 1107 50-51 -8.016607957558845 1107 52-53 0.10013618754812015 1107 54-55 4.203184590117658 1107 56-57 5.337531084430982 1107 58-59 5.571343604155576 1107 60-61 5.625409962560841 1107 62-63 5.583232017597862 1107 64-65 5.446558768053894 1107 66-67 5.412589667094082 1107 68-69 5.437719308169461 1107 70-71 5.327511868469694 1107 72-73 5.087893775004838 1107 74-75 5.22851558188203 1107 76-77 5.035893892081061 1107 78-79 5.014414129486122 1107 80-81 4.859301292158113 1107 82-83 4.644820849992309 1107 84-85 4.61599294386021 1107 86-87 4.4016958341295815 1107 88-89 4.062965949938473 1107 90-91 3.8562788373286665 1107 92-93 3.653484127806353 1107 94-95 3.311112895828268 1107 96-97 3.0441122192284134 1107 98-99 2.6797997967496654 1107 100-101 2.387165808145898 1108 1 5.979714384130503 1108 2 6.036004473972255 1108 3 6.372191188973169 1108 4 7.6476387377979655 1108 5 7.861009052693706 1108 6 7.2152256887529695 1108 7 7.360178622501735 1108 8 6.61844431532743 1108 9 5.01490504637 1108 10-11 5.789013131991695 1108 12-13 5.250146228155678 1108 14-15 5.880003945873131 1108 16-17 5.981652506756532 1108 18-19 6.418246331497151 1108 20-21 7.286441849670261 1108 22-23 7.488405285848241 1108 24-25 6.86245502079878 1108 26-27 7.0642229405905645 1108 28-29 7.0466251326877085 1108 30-31 7.088329814204009 1108 32-33 6.829781998742057 1108 34-35 8.74147161625027 1108 36-37 -2.8464517611762314 1108 38-39 -1.9880757075408493 1108 40-41 4.699052211627777 1108 42-43 6.69702844437332 1108 44-45 -2.705516136386837 1108 46-47 -3.5691207036866572 1108 48-49 4.129581189071823 1108 50-51 -8.062204544766805 1108 52-53 0.4311879769189062 1108 54-55 4.716037816879105 1108 56-57 5.986396437098438 1108 58-59 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-14.631678630225114 2313 62-63 -14.152630993194093 2313 64-65 -13.654446572819495 2313 66-67 -13.213530022668118 2313 68-69 -12.765803466206188 2313 70-71 -12.195279264233001 2313 72-73 -11.704074929812805 2313 74-75 -11.18798197220521 2313 76-77 -10.674541012015142 2313 78-79 -10.184422632977425 2313 80-81 -9.694815350082322 2313 82-83 -9.245260393952535 2313 84-85 -8.74706976411228 2313 86-87 -8.233482944081892 2313 88-89 -7.762592728117232 2313 90-91 -7.264153580966828 2313 92-93 -6.7343253019521985 2313 94-95 -6.185431244919455 2313 96-97 -5.576057432043971 2313 98-99 -4.946024896935538 2313 100-101 -4.196717320977569 2314 1 -19.381264325751925 2314 2 -18.424434038897143 2314 3 -18.8000363292982 2314 4 -20.636759101413983 2314 5 -20.63025246621319 2314 6 -21.213754292181253 2314 7 -21.20512166348492 2314 8 -21.7860679185461 2314 9 -22.73374446109743 2314 10-11 -22.83030685529779 2314 12-13 -22.64864627263873 2314 14-15 -22.769185758607325 2314 16-17 -21.94399096321487 2314 18-19 -21.36043177463567 2314 20-21 -20.845112011149567 2314 22-23 -20.670952832995102 2314 24-25 -20.321053082156414 2314 26-27 -19.897327742599774 2314 28-29 -19.724426662672954 2314 30-31 -19.54290628366698 2314 32-33 -19.131768684448282 2314 34-35 -16.63714293729279 2314 36-37 -15.130531837439339 2314 38-39 -14.248323562640945 2314 40-41 -15.176066949478038 2314 42-43 -15.020162531158935 2314 44-45 -13.34692064862071 2314 46-47 -13.874490897522056 2314 48-49 -13.327044168411495 2314 50-51 -11.739039625796357 2314 52-53 -14.089307733281284 2314 54-55 -15.295401649279139 2314 56-57 -15.384115161249195 2314 58-59 -15.011911186922024 2314 60-61 -14.63315114331412 2314 62-63 -14.158521045550113 2314 64-65 -13.654446572819495 2314 66-67 -13.217947561935134 2314 68-69 -12.767275979295194 2314 70-71 -12.202805442243472 2314 72-73 -11.713237233477725 2314 74-75 -11.198125951262801 2314 76-77 -10.679940226674828 2314 78-79 -10.18916739737533 2314 80-81 -9.704632104009024 2314 82-83 -9.256058823271907 2314 84-85 -8.757868193431653 2314 86-87 -8.24313608544315 2314 88-89 -7.772409482043933 2314 90-91 -7.273315884631749 2314 92-93 -6.739070066350104 2314 94-95 -6.185431244919455 2314 96-97 -5.576057432043971 2314 98-99 -4.946024896935538 2314 100-101 -4.196717320977569 2315 1 -19.381264325751925 2315 2 -18.424434038897143 2315 3 -18.8000363292982 2315 4 -17.289591775985787 2315 5 -20.63025246621319 2315 6 -21.213754292181253 2315 7 -21.20512166348492 2315 8 -21.7860679185461 2315 9 -19.629001378093477 2315 10-11 -18.46518827822269 2315 12-13 -16.131518472902233 2315 14-15 -16.145503281663977 2315 16-17 -19.34847054160749 2315 18-19 -21.36043177463567 2315 20-21 -20.845112011149567 2315 22-23 -20.670952832995102 2315 24-25 -20.321053082156414 2315 26-27 -19.897327742599774 2315 28-29 -19.724426662672954 2315 30-31 -19.54290628366698 2315 32-33 -19.131768684448282 2315 34-35 -16.63714293729279 2315 36-37 -15.130531837439339 2315 38-39 -14.248323562640945 2315 40-41 -15.176066949478038 2315 42-43 -15.020162531158935 2315 44-45 -13.34692064862071 2315 46-47 -13.874490897522056 2315 48-49 -13.327044168411495 2315 50-51 -11.739039625796357 2315 52-53 -14.089307733281284 2315 54-55 -15.295401649279139 2315 56-57 -15.384115161249195 2315 58-59 -15.011911186922024 2315 60-61 -14.63315114331412 2315 62-63 -14.158521045550113 2315 64-65 -13.654446572819495 2315 66-67 -13.217947561935134 2315 68-69 -12.767275979295194 2315 70-71 -12.202805442243472 2315 72-73 -11.713237233477725 2315 74-75 -11.198125951262801 2315 76-77 -10.679940226674828 2315 78-79 -10.18916739737533 2315 80-81 -9.704632104009024 2315 82-83 -9.256058823271907 2315 84-85 -8.757868193431653 2315 86-87 -8.24313608544315 2315 88-89 -7.772409482043933 2315 90-91 -7.273315884631749 2315 92-93 -6.739070066350104 2315 94-95 -6.185431244919455 2315 96-97 -5.576057432043971 2315 98-99 -4.946024896935538 2315 100-101 -4.196717320977569 2316 1 -19.381264325751925 2316 2 -18.424434038897143 2316 3 -18.8000363292982 2316 4 -20.636759101413983 2316 5 -20.63025246621319 2316 6 -21.213754292181253 2316 7 -21.20512166348492 2316 8 -21.7860679185461 2316 9 -22.73374446109743 2316 10-11 -22.83030685529779 2316 12-13 -22.64864627263873 2316 14-15 -22.769185758607325 2316 16-17 -21.94399096321487 2316 18-19 -21.36043177463567 2316 20-21 -20.845112011149567 2316 22-23 -20.670952832995102 2316 24-25 -20.321053082156414 2316 26-27 -19.897327742599774 2316 28-29 -19.724426662672954 2316 30-31 -19.54290628366698 2316 32-33 -19.131768684448282 2316 34-35 -16.63714293729279 2316 36-37 -15.130531837439339 2316 38-39 -14.248323562640945 2316 40-41 -15.176066949478038 2316 42-43 -15.020162531158935 2316 44-45 -13.34692064862071 2316 46-47 -13.874490897522056 2316 48-49 -13.327044168411495 2316 50-51 -11.739039625796357 2316 52-53 -14.089307733281284 2316 54-55 -15.295401649279139 2316 56-57 -15.384115161249195 2316 58-59 -15.011911186922024 2316 60-61 -14.63315114331412 2316 62-63 -14.158521045550113 2316 64-65 -13.654446572819495 2316 66-67 -13.217947561935134 2316 68-69 -12.767275979295194 2316 70-71 -12.202805442243472 2316 72-73 -11.713237233477725 2316 74-75 -11.198125951262801 2316 76-77 -10.679940226674828 2316 78-79 -10.18916739737533 2316 80-81 -9.704632104009024 2316 82-83 -9.256058823271907 2316 84-85 -8.757868193431653 2316 86-87 -8.24313608544315 2316 88-89 -7.772409482043933 2316 90-91 -7.273315884631749 2316 92-93 -6.739070066350104 2316 94-95 -6.185431244919455 2316 96-97 -5.576057432043971 2316 98-99 -4.946024896935538 2316 100-101 -4.196717320977569 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 579239.0 3 41907.0 4 70665.0 5 55165.0 6 51852.0 7 62553.0 8 76397.0 9 85899.0 10 84731.0 11 74877.0 12 63336.0 13 55687.0 14 52723.0 15 54613.0 16 58832.0 17 61718.0 18 63877.0 19 66258.0 20 68449.0 21 71004.0 22 73509.0 23 76395.0 24 79305.0 25 82633.0 26 85019.0 27 87000.0 28 89964.0 29 91134.0 30 90799.0 31 86741.0 32 81095.0 33 73085.0 34 61346.0 35 44798.0 36 24152.0 37 6599.0 38 515.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.354318609648416 19.551550787469658 13.47490493314012 24.619225669741805 2 16.378096457237753 20.242137766399114 39.092253667312484 24.28751210905065 3 19.5712533834534 24.887190849967833 28.836198774012615 26.705356992566152 4 13.50541709483924 16.241034657667814 36.425505856875155 33.82804239061778 5 14.581907894305399 36.881100787957195 33.81894078932124 14.718050528416168 6 32.4876699438658 35.20843473182128 17.630885168240372 14.673010156072552 7 28.593832869762632 31.344059055467692 21.68698938227104 18.375118692498635 8 26.798445767067815 34.16607788344491 20.13093042710451 18.904545922382766 9 27.505897365808135 15.36972636363189 19.835702937193844 37.28867333336613 10-11 26.370245096985762 25.051968690935354 27.38565157829288 21.192134633786004 12-13 26.54080420748901 22.823306939968816 27.38322755498343 23.25266129755875 14-15 23.81418391437407 24.02820461474895 25.066275806282857 27.09133566459413 16-17 23.82481173130304 26.758906296949426 25.763451121461067 23.65283085028647 18-19 23.813715521098253 25.931953019114633 27.29584740948669 22.958484050300427 20-21 24.636963858130876 25.253675674042785 27.06269966812754 23.046660799698795 22-23 24.413320962937643 25.151403877142435 26.723863469895992 23.711411690023926 24-25 24.110929124748402 25.4709324556151 26.754905133658063 23.66323328597844 26-27 23.87822414578819 25.694633293952712 26.844810702249074 23.58233185801002 28-29 23.842592818870187 25.835343927425846 26.87737940394398 23.44468384975999 30-31 24.162779684557805 25.624708710755034 26.631293797022053 23.5812178076651 32-33 24.073255996909804 25.72958093948758 26.489151035734192 23.70801202786843 34-35 24.34659374858623 25.627366584847756 26.64302931277375 23.38301035379227 36-37 24.787876674793107 25.696446626991044 26.225916817081796 23.289759881134053 38-39 24.62043740674704 25.744384623722162 26.227914817979713 23.407263151551085 40-41 24.619741999117505 25.579380694058713 26.43642121109871 23.364456095725075 42-43 24.751269195591476 25.520472358783902 26.148737919745223 23.579520525879392 44-45 24.82057899272199 25.800483484925262 25.941522107809682 23.437415414543064 46-47 24.23850865872085 25.63513442086558 26.272272881797914 23.854084038615657 48-49 24.727729735852307 25.85057453590013 25.86701243281091 23.554683295436654 50-51 24.769197011217535 25.977124780222304 25.83362334963106 23.420054858929095 52-53 24.043161778289488 25.941526510531926 26.178810124816316 23.836501586362267 54-55 23.83920122814724 25.848756563365516 26.3565557159408 23.955486492546445 56-57 23.96688857906716 26.028880698278833 26.06752000471978 23.93671071793423 58-59 23.865196546648576 25.99887186698161 26.331657364052212 23.8042742223176 60-61 23.891091030182825 26.073755968940542 26.21563562573804 23.819517375138588 62-63 23.86394403474496 25.988644069811933 26.22368441827514 23.92372747716797 64-65 23.708318065225356 25.82671254893441 26.283522500011347 24.181446885828887 66-67 23.875119388082602 26.006721737787185 25.965315802045946 24.152843072084266 68-69 23.75672903881395 25.922184850642953 26.13973523372123 24.181350876821863 70-71 23.6754800229092 25.675696001882102 26.182803059959237 24.466020915249466 72-73 23.9457431357963 25.826811155055037 26.031787062487492 24.195658646661172 74-75 23.929370764198808 25.99605651964093 25.83040964588788 24.244163070272375 76-77 23.921487554172536 25.823428310829442 25.96609208650423 24.288992048493792 78-79 23.81143002117684 25.97265022926965 25.904321072677217 24.311598676876294 80-81 23.928605816447966 25.91379316985245 25.83668729755857 24.32091371614101 82-83 24.071497848093593 25.83638313026611 25.89298433048433 24.19913469115597 84-85 24.04918497785022 25.958904407683836 25.671243965838762 24.320666648627178 86-87 23.776069653372367 26.010669576470764 25.750344948608767 24.462915821548105 88-89 23.896188746613422 25.81061274272952 25.721253706128504 24.57194480452855 90-91 23.924358071668266 25.77857217761266 25.665504404362416 24.631565346356663 92-93 24.00824356818453 25.59714461913608 25.80112774677909 24.5934840659003 94-95 23.813634955636864 25.506369246056693 25.813215553013414 24.86678024529303 96-97 23.842007690195004 25.526774543013214 25.743118496139644 24.88809927065214 98-99 23.701366333413688 25.543206928737327 25.596864047940294 25.158562689908692 100-101 24.337660758088255 25.5750745824498 25.252152448834124 24.835112210627816 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32793.0 1 36238.5 2 26956.0 3 14453.5 4 18692.0 5 24707.0 6 26879.5 7 26071.5 8 23989.5 9 21964.5 10 19937.5 11 17772.5 12 15561.5 13 13217.0 14 11088.5 15 9355.0 16 7924.0 17 6831.0 18 6156.5 19 5933.5 20 6019.0 21 6404.5 22 7067.0 23 7909.5 24 9158.0 25 10910.5 26 12960.5 27 15415.0 28 18566.5 29 22343.5 30 26808.0 31 31928.5 32 37671.5 33 43202.5 34 48341.0 35 53440.5 36 58807.0 37 63739.5 38 67182.0 39 70334.0 40 72908.5 41 75389.5 42 78740.0 43 82370.5 44 86171.5 45 89835.0 46 92911.0 47 95924.0 48 98335.5 49 98954.5 50 98305.5 51 97084.5 52 95580.0 53 93341.0 54 89848.5 55 84814.5 56 78477.5 57 71396.5 58 63903.5 59 56712.5 60 49496.5 61 42261.0 62 35579.5 63 29218.5 64 23338.5 65 18513.5 66 14660.0 67 11506.0 68 9047.0 69 7109.5 70 5659.0 71 4466.0 72 3462.0 73 2722.0 74 2099.0 75 1617.5 76 1243.5 77 878.5 78 583.5 79 383.5 80 263.5 81 164.5 82 98.0 83 64.0 84 40.0 85 22.0 86 12.5 87 8.5 88 6.0 89 4.5 90 2.5 91 1.5 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.265716341504897 2 21.185409184378464 3 16.978308195344518 4 22.186202333911524 5 22.037968241979115 6 15.963642598026567 7 14.783018896864316 8 11.910774597264435 9 2.988779677920619 10-11 6.423931778914765 12-13 3.5145011389381886 14-15 7.319454523082629 16-17 11.350269762869763 18-19 14.406946040268274 20-21 14.812144220284928 22-23 12.74758655197413 24-25 15.271059790249952 26-27 12.827907002109498 28-29 12.828725033428508 30-31 13.666320934818923 32-33 12.157700759371656 34-35 19.00867897144832 36-37 23.747824121903026 38-39 22.798004548935825 40-41 17.926321282482235 42-43 17.846256467134058 44-45 23.18556392863631 46-47 12.62159268652733 48-49 21.309153463697985 50-51 21.012310348811962 52-53 11.069293047108719 54-55 12.66719793256218 56-57 12.18461058130328 58-59 13.19714588872797 60-61 12.614059981464774 62-63 12.569784036323316 64-65 13.63373602061166 66-67 11.960640423085799 68-69 12.99071227691178 70-71 11.623816027483127 72-73 10.99538050897227 74-75 11.07784829132004 76-77 11.13524682220396 78-79 11.532486239179406 80-81 11.501622599205897 82-83 10.032915535198693 84-85 9.936404881874573 86-87 10.0698462407882 88-89 11.469003600360343 90-91 8.991288988991684 92-93 10.98829090420751 94-95 11.091175384892257 96-97 10.64643902445675 98-99 10.49602351567365 100-101 10.603884353549047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2933873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.60667415252897 #Duplication Level Percentage of deduplicated Percentage of total 1 99.07649293212484 96.70526961801248 2 0.660398738663342 1.289186489909079 3 0.12005123225200152 0.3515340452409207 4 0.0490100260497859 0.19134822571393634 5 0.022882373082906245 0.11167361666699148 6 0.015699769201056795 0.09194413540064766 7 0.009499031144259337 0.06490181863637118 8 0.006016450496589245 0.046979657854032596 9 0.0067321468371001406 0.05913922164082308 >10 0.029777368558747172 0.5171713015485276 >50 0.002135383564810222 0.14240366985457803 >100 0.0011997576960598906 0.18818694875682038 >500 6.986021902501633E-5 0.05468696554947741 >1k 0.0 0.0 >5k 3.49301095124337E-5 0.1855742852152182 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5443 0.18552268622397766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.408463829211421E-5 2 0.0 0.0 0.0 0.0 3.408463829211421E-5 3 0.0 0.0 0.0 0.0 3.408463829211421E-5 4 0.0 0.0 0.0 0.0 3.408463829211421E-5 5 0.0 0.0 0.0 3.408463829211421E-5 3.408463829211421E-5 6 0.0 0.0 0.0 3.408463829211421E-5 6.816927658422842E-5 7 0.0 0.0 0.0 3.408463829211421E-5 6.816927658422842E-5 8 0.0 0.0 0.0 3.408463829211421E-5 6.816927658422842E-5 9 0.0 0.0 0.0 3.408463829211421E-5 6.816927658422842E-5 10-11 0.0 0.0 0.0 3.408463829211421E-5 6.816927658422842E-5 12-13 1.7042319146057106E-5 0.0 0.0 3.408463829211421E-5 1.3633855316845685E-4 14-15 3.408463829211421E-5 0.0 0.0 3.408463829211421E-5 1.7042319146057106E-4 16-17 3.408463829211421E-5 0.0 0.0 3.408463829211421E-5 1.7042319146057106E-4 18-19 3.408463829211421E-5 0.0 0.0 3.408463829211421E-5 2.0450782975268527E-4 20-21 3.408463829211421E-5 0.0 0.0 6.816927658422842E-5 2.556347871908566E-4 22-23 3.408463829211421E-5 5.112695743817132E-5 0.0 1.3633855316845685E-4 3.578887020671992E-4 24-25 3.408463829211421E-5 6.816927658422842E-5 0.0 2.215501488987424E-4 3.749310212132563E-4 26-27 3.408463829211421E-5 6.816927658422842E-5 0.0 3.23804063775085E-4 3.749310212132563E-4 28-29 3.408463829211421E-5 6.816927658422842E-5 0.0 5.112695743817132E-4 3.749310212132563E-4 30-31 3.408463829211421E-5 6.816927658422842E-5 0.0 0.002385924680447995 3.749310212132563E-4 32-33 3.408463829211421E-5 6.816927658422842E-5 0.0 0.008367778700714038 3.749310212132563E-4 34-35 3.408463829211421E-5 6.816927658422842E-5 0.0 0.017280911614101904 3.749310212132563E-4 36-37 3.408463829211421E-5 6.816927658422842E-5 0.0 0.02827320746330874 3.749310212132563E-4 38-39 3.408463829211421E-5 6.816927658422842E-5 0.0 0.04511101877961316 3.749310212132563E-4 40-41 3.408463829211421E-5 6.816927658422842E-5 0.0 0.06522095537196054 3.749310212132563E-4 42-43 3.408463829211421E-5 6.816927658422842E-5 0.0 0.08706920851720576 3.749310212132563E-4 44-45 3.408463829211421E-5 6.816927658422842E-5 0.0 0.11570030468258169 3.749310212132563E-4 46-47 3.408463829211421E-5 6.816927658422842E-5 0.0 0.14922254644287603 3.9197334035931343E-4 48-49 5.112695743817132E-5 6.816927658422842E-5 0.0 0.18201196847988987 4.4310029779748473E-4 50-51 6.816927658422842E-5 6.816927658422842E-5 0.0 0.21914718189914834 5.623965318198845E-4 52-53 6.816927658422842E-5 6.816927658422842E-5 0.0 0.27284752952837427 6.305658084041129E-4 54-55 6.816927658422842E-5 6.816927658422842E-5 0.0 0.3371311573473017 6.4760812755017E-4 56-57 6.816927658422842E-5 6.816927658422842E-5 0.0 0.40637410003773167 6.646504466962271E-4 58-59 6.816927658422842E-5 6.816927658422842E-5 0.0 0.47875282945103625 6.816927658422842E-4 60-61 6.816927658422842E-5 6.816927658422842E-5 0.0 0.5559034082252368 6.816927658422842E-4 62-63 6.816927658422842E-5 6.816927658422842E-5 0.0 0.6343321609353916 7.157774041343985E-4 64-65 6.816927658422842E-5 6.816927658422842E-5 0.0 0.7214695387291815 7.157774041343985E-4 66-67 8.521159573028553E-5 6.816927658422842E-5 0.0 0.8221896448823791 7.328197232804555E-4 68-69 1.0225391487634264E-4 6.816927658422842E-5 0.0 0.926659061247709 7.498620424265126E-4 70-71 1.0225391487634264E-4 6.816927658422842E-5 0.0 1.0299525575919612 7.839466807186269E-4 72-73 1.0225391487634264E-4 6.816927658422842E-5 0.0 1.1438975034024992 8.180313190107411E-4 74-75 1.0225391487634264E-4 6.816927658422842E-5 0.0 1.2616088017443157 8.350736381567982E-4 76-77 1.0225391487634264E-4 6.816927658422842E-5 0.0 1.400878633805894 8.521159573028553E-4 78-79 1.0225391487634264E-4 6.816927658422842E-5 3.408463829211421E-5 1.5510555501209493 8.862005955949695E-4 80-81 1.0225391487634264E-4 6.816927658422842E-5 3.408463829211421E-5 1.7030730369037788 9.032429147410266E-4 82-83 1.0225391487634264E-4 1.0225391487634264E-4 3.408463829211421E-5 1.8560278512396413 9.202852338870837E-4 84-85 1.0225391487634264E-4 1.0225391487634264E-4 3.408463829211421E-5 2.018662702850464 9.202852338870837E-4 86-87 1.0225391487634264E-4 1.0225391487634264E-4 3.408463829211421E-5 2.198800016224288 9.543698721791978E-4 88-89 1.0225391487634264E-4 1.0225391487634264E-4 3.408463829211421E-5 2.386572288575545 9.88454510471312E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2195 0.0 50.45842 1 GTATCAA 4095 0.0 44.381172 1 ATCAACG 5270 0.0 34.946857 3 TATCAAC 5400 0.0 33.925358 2 TCAACGC 5435 0.0 33.78563 4 CAACGCA 5450 0.0 32.364902 5 GTACATG 8155 0.0 30.249458 1 AACGCAG 6070 0.0 29.620264 6 TACATGG 8325 0.0 29.078882 2 ACATGGG 9015 0.0 27.62976 3 ACGCAGA 7040 0.0 24.580158 7 CGCAGAG 7305 0.0 24.18705 8 CATGGGG 7100 0.0 24.09125 4 GCAGAGT 7445 0.0 21.411224 9 AGAGTAC 6645 0.0 18.949022 10-11 ATGGGGG 4290 0.0 18.812092 5 GAGTACA 5855 0.0 18.400618 1 AGTACAT 5825 0.0 18.144299 2 CAGAGTA 7110 0.0 18.020985 10-11 GTGGTAT 1130 0.0 17.375273 1 >>END_MODULE