FastQCFastQC Report
Sat 18 Jun 2016
SRR1930970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933873
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.10007249802564734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.054.4618451
GTATCAA56250.043.218741
CAACGCA74500.031.405135
ATCAACG74650.031.352183
TCAACGC76100.030.8790384
TATCAAC79200.029.8507182
AACGCAG79150.029.7394756
GTACATG113500.026.2717171
TACATGG116500.025.3463292
ACGCAGA92450.025.3071127
ACATGGG118150.024.5101933
CGCAGAG97000.024.0710988
CATGGGG86750.023.2574794
GCAGAGT108200.020.9654039
GTGGTAT14150.020.4690691
GAGTACA83850.020.1024951
AGAGTAC99900.019.47694810-11
AGTACAT86550.018.86872
CAGAGTA104650.018.2074410-11
GAGTACT50750.017.7204912-13
Produced by FastQC (version 0.11.5)