FastQCFastQC Report
Sat 18 Jun 2016
SRR1930969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226593
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59920.18570671913067438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.047.7369271
GTATCAA38650.046.0605661
TCAACGC48000.037.0681844
ATCAACG47650.036.927383
CAACGCA49200.035.5907635
TATCAAC50800.034.5426752
AACGCAG55600.032.6326566
GTACATG63550.029.5210271
TACATGG63800.028.3735852
ACGCAGA64000.027.9380267
CGCAGAG67550.026.3482678
ACATGGG70500.025.6023523
CATGGGG55050.022.3408854
GCAGAGT75850.021.813899
AGAGTAC67150.018.91517310-11
GTGTAGC10050.017.5632971
CAGAGTA71300.017.34949910-11
GAGTACT46350.017.01643612-13
GAGTACA44600.016.2828981
ATGGGGG38200.016.208415
Produced by FastQC (version 0.11.5)