FastQCFastQC Report
Sat 18 Jun 2016
SRR1930969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226593
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.13642873458164695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.11110790855865613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.054.9002151
GTATCAA59600.040.1588551
TCAACGC73700.031.437534
ATCAACG74250.031.2065933
CAACGCA74500.031.1544975
TATCAAC76500.030.5384392
AACGCAG78150.029.9409736
GTACATG89300.027.9193251
TACATGG91950.026.2850762
ACATGGG91200.025.6669983
ACGCAGA92850.025.0980997
CGCAGAG95600.024.3761358
CATGGGG67450.021.8914494
GCAGAGT111500.020.5169779
AGAGTAC102250.019.40234610-11
ATGGGGG40900.018.8000875
CCGTCGA1801.2418964E-418.4572419
TATACTG16450.018.1778625
CAGAGTA106600.018.00953710-11
GTACTAG7200.017.1489641
Produced by FastQC (version 0.11.5)