##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930969_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3226593 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.236739185884307 31.0 31.0 33.0 30.0 34.0 2 31.35226475728423 31.0 31.0 34.0 30.0 34.0 3 31.437092933629994 31.0 31.0 34.0 28.0 34.0 4 35.31066608028964 37.0 35.0 37.0 33.0 37.0 5 35.09513688277387 35.0 35.0 37.0 32.0 37.0 6 35.132400646750305 37.0 35.0 37.0 32.0 37.0 7 35.01194479750003 36.0 35.0 37.0 32.0 37.0 8 34.98369115658529 36.0 35.0 37.0 32.0 37.0 9 36.4895092129686 38.0 35.0 39.0 32.0 39.0 10-11 36.41490342909688 38.0 35.0 39.0 32.0 39.0 12-13 36.30609810409928 38.0 35.0 39.0 32.0 39.0 14-15 37.32249093703483 39.0 36.0 41.0 32.0 41.0 16-17 37.28431274102435 39.0 36.0 40.5 32.0 41.0 18-19 37.26309206026295 39.0 36.0 40.0 31.5 41.0 20-21 37.22185305057068 39.0 36.0 40.0 31.0 41.0 22-23 37.09124035786354 39.0 36.0 40.0 31.0 41.0 24-25 36.96408750654328 39.0 36.0 40.0 31.0 41.0 26-27 36.68169288782316 38.5 35.0 40.0 30.0 41.0 28-29 36.48931365065256 38.0 35.0 40.0 30.0 41.0 30-31 36.24737765190714 38.0 35.0 40.0 30.0 41.0 32-33 36.00997243842034 38.0 34.5 40.0 29.5 41.0 34-35 36.207367027697636 38.0 35.0 40.0 30.0 41.0 36-37 36.25747452498657 38.0 35.0 40.0 30.0 41.0 38-39 36.22581295502718 38.0 35.0 40.0 30.0 41.0 40-41 36.127386534341326 38.0 35.0 40.0 30.0 41.0 42-43 35.98689949429631 38.0 35.0 40.0 29.0 41.0 44-45 35.80402285010846 38.0 34.5 40.0 28.5 41.0 46-47 35.58840114015 38.0 34.0 40.0 28.0 41.0 48-49 35.390775037322655 38.0 34.0 40.0 27.0 41.0 50-51 35.1176352270026 38.0 33.5 40.0 26.5 41.0 52-53 34.89676479184081 38.0 33.0 40.0 26.0 41.0 54-55 34.716252406175805 38.0 33.0 40.0 26.0 41.0 56-57 34.451330397109274 37.0 33.0 40.0 26.0 41.0 58-59 34.202452246068844 37.0 33.0 40.0 25.5 41.0 60-61 33.88011813079616 36.5 32.0 39.5 24.5 41.0 62-63 33.5648316970873 36.0 32.0 39.0 24.0 41.0 64-65 33.252311648850664 36.0 31.5 39.0 23.0 40.5 66-67 32.908189691107616 35.0 31.0 39.0 23.0 40.0 68-69 32.52562393211663 35.0 31.0 38.0 22.0 40.0 70-71 32.11362666441042 35.0 31.0 38.0 22.0 40.0 72-73 31.685916383008333 34.5 30.0 37.0 20.5 39.0 74-75 31.160588583685637 34.0 30.0 36.5 19.5 39.0 76-77 29.793937134308543 32.5 28.5 35.0 18.0 37.5 78-79 30.555098055441142 34.0 29.5 36.0 19.0 38.0 80-81 30.560183915355918 34.0 30.0 35.0 19.0 37.0 82-83 30.28759236135453 34.0 30.0 35.0 18.0 37.0 84-85 29.969353277590326 34.0 29.0 35.0 17.5 36.0 86-87 29.634161172481313 34.0 29.0 35.0 14.0 36.0 88-89 29.296721185473345 34.0 29.0 35.0 8.5 36.0 90-91 28.95226202994924 34.0 29.0 35.0 6.5 35.0 92-93 28.598618108946496 33.0 29.0 35.0 2.0 35.0 94-95 28.169319309872677 33.0 28.0 35.0 2.0 35.0 96-97 27.697763089425905 33.0 27.0 35.0 2.0 35.0 98-99 27.20093749041171 33.0 27.0 35.0 2.0 35.0 100-101 25.963932234403288 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4978118535106866 1101 2 0.6163395825187479 1101 3 0.5564365574242025 1101 4 0.34731627374852536 1101 5 0.50792677555431 1101 6 0.47160912028581947 1101 7 0.49910172639575734 1101 8 0.45464149159907663 1101 9 0.6992412355495503 1101 10-11 0.6076532221679898 1101 12-13 0.6504670663176313 1101 14-15 0.7861147605865213 1101 16-17 0.7381039498168889 1101 18-19 0.801066514692657 1101 20-21 0.7277272580976231 1101 22-23 0.8117217716766163 1101 24-25 0.7752840021293679 1101 26-27 0.6990513995247198 1101 28-29 0.6389247643409988 1101 30-31 0.6967843736497912 1101 32-33 0.8073964377709473 1101 34-35 0.7647213980992191 1101 36-37 0.844334149779165 1101 38-39 0.9842739857987937 1101 40-41 0.9120649637193807 1101 42-43 0.9228680325629384 1101 44-45 1.0008237939891487 1101 46-47 0.9129932523753439 1101 48-49 0.9391098220738954 1101 50-51 0.7626585832300634 1101 52-53 0.8965906788734017 1101 54-55 0.8221396665030554 1101 56-57 1.016756412872006 1101 58-59 0.9234884439339339 1101 60-61 0.9009174282176176 1101 62-63 0.9669577160170277 1101 64-65 0.9774260991644468 1101 66-67 1.1182874050306637 1101 68-69 0.9348943386077266 1101 70-71 0.8276144283009259 1101 72-73 0.8078828150246515 1101 74-75 0.9649921717591354 1101 76-77 1.0560594506622643 1101 78-79 1.0423398646577446 1101 80-81 0.9760791445611083 1101 82-83 1.1888919955635089 1101 84-85 1.2022807256824244 1101 86-87 1.1499806448795091 1101 88-89 1.0643430680971129 1101 90-91 0.9793148823495699 1101 92-93 1.0652982900648418 1101 94-95 1.22115032665927 1101 96-97 1.1306796652384143 1101 98-99 1.1983169931960838 1101 100-101 1.2750677437343114 1102 1 0.4168216397272353 1102 2 0.528092566795415 1102 3 0.5014891030774038 1102 4 0.3342069084209953 1102 5 0.3921384985528533 1102 6 0.4414341760566316 1102 7 0.44305381435704305 1102 8 0.481063265967677 1102 9 0.615980267191901 1102 10-11 0.6549655953085818 1102 12-13 0.6879527122551039 1102 14-15 0.7773758376265008 1102 16-17 0.6657913535243907 1102 18-19 0.7470618403713374 1102 20-21 0.6835743726551797 1102 22-23 0.7963987884720609 1102 24-25 0.851911081157219 1102 26-27 0.8033874225496831 1102 28-29 0.8727789391306402 1102 30-31 0.9399699764968759 1102 32-33 0.8837788834550295 1102 34-35 0.7772133928310652 1102 36-37 0.7929239229087202 1102 38-39 0.8623249505556103 1102 40-41 0.8283693641082479 1102 42-43 0.7059094979147034 1102 44-45 0.7201183122306674 1102 46-47 0.8290184921802677 1102 48-49 0.6948947287458722 1102 50-51 0.7620840283739767 1102 52-53 0.7240245623963446 1102 54-55 0.666298859052155 1102 56-57 0.5728926538305501 1102 58-59 0.7372196723219133 1102 60-61 0.6789060477646061 1102 62-63 0.4907900945054138 1102 64-65 0.46710142441938274 1102 66-67 0.6764930243586207 1102 68-69 0.626047341462403 1102 70-71 0.5806196469167162 1102 72-73 0.5807410005652152 1102 74-75 0.7763780000337874 1102 76-77 0.8983560359280212 1102 78-79 0.9320825670754083 1102 80-81 0.8649601373055837 1102 82-83 0.9594728055392139 1102 84-85 0.9203893018859581 1102 86-87 0.8511967197816475 1102 88-89 0.864943891120582 1102 90-91 0.8952578256928234 1102 92-93 1.1246283497842136 1102 94-95 1.0344404989806044 1102 96-97 0.9559008627274572 1102 98-99 0.9518937749610714 1102 100-101 1.160284974514287 1103 1 0.44801253073866576 1103 2 0.49850667320154685 1103 3 0.4990611776185716 1103 4 0.27365935435400246 1103 5 0.33157857179153183 1103 6 0.3680301457233881 1103 7 0.3989021903449981 1103 8 0.4164928598220001 1103 9 0.5162731355804837 1103 10-11 0.5508354652562204 1103 12-13 0.6316672156904488 1103 14-15 0.7721422838386118 1103 16-17 0.6242017404492302 1103 18-19 0.6678983687851385 1103 20-21 0.708111295796293 1103 22-23 0.7094663198845552 1103 24-25 0.7545644486161649 1103 26-27 0.8397037741202737 1103 28-29 0.7929069629475975 1103 30-31 0.9353481996916528 1103 32-33 0.8979203935088051 1103 34-35 0.9164459596807788 1103 36-37 0.9273461972113566 1103 38-39 0.8840952337416397 1103 40-41 0.9656411946004155 1103 42-43 0.8467330589775415 1103 44-45 0.9764995772859137 1103 46-47 0.8477668646674275 1103 48-49 0.835167273305558 1103 50-51 0.9640999143380213 1103 52-53 0.8972936440403032 1103 54-55 1.0431905984563556 1103 56-57 0.93366190931566 1103 58-59 0.9943461284513688 1103 60-61 0.9644056816191764 1103 62-63 0.9072757985285094 1103 64-65 0.859209851423131 1103 66-67 0.7620859809386147 1103 68-69 0.8114472744196561 1103 70-71 0.7478703239380877 1103 72-73 0.7899315146410721 1103 74-75 0.98301029171094 1103 76-77 1.1253778085131039 1103 78-79 1.0740002483749471 1103 80-81 1.0411806340644638 1103 82-83 0.8711961731947895 1103 84-85 0.911836700910623 1103 86-87 1.02832056709493 1103 88-89 1.0866835303078801 1103 90-91 1.1211529157952889 1103 92-93 1.1206232414919661 1103 94-95 1.1167689345493201 1103 96-97 1.082231059247082 1103 98-99 1.0927194460958134 1103 100-101 1.375981559602696 1104 1 0.4208304215981684 1104 2 0.5280106233472353 1104 3 0.3969627133160536 1104 4 0.18094715226862945 1104 5 0.42419529872380224 1104 6 0.41768814206812976 1104 7 0.4854951012223694 1104 8 0.513310956064096 1104 9 0.5525153001650267 1104 10-11 0.4922473040826816 1104 12-13 0.6517184780764254 1104 14-15 0.6104591827094268 1104 16-17 0.6150644815020243 1104 18-19 0.7015566907815654 1104 20-21 0.6894325349934647 1104 22-23 0.5555991829965947 1104 24-25 0.588335962327541 1104 26-27 0.715012067737824 1104 28-29 0.5469779559400649 1104 30-31 0.6131093813631949 1104 32-33 0.7798585668711482 1104 34-35 0.7334816134519784 1104 36-37 0.7428510567763809 1104 38-39 0.8107831757016299 1104 40-41 0.7026353185303051 1104 42-43 0.6762238720382143 1104 44-45 0.8194874037413129 1104 46-47 0.7228842542361207 1104 48-49 0.67026586389062 1104 50-51 0.7433950357149683 1104 52-53 0.7180340945590515 1104 54-55 0.80358510882008 1104 56-57 0.9266542458618758 1104 58-59 0.8407198535412022 1104 60-61 0.8059762293847328 1104 62-63 0.6626467153351356 1104 64-65 0.8638688908709256 1104 66-67 0.8262078153752626 1104 68-69 0.8049302625884209 1104 70-71 0.7331916667517078 1104 72-73 0.6868196907557227 1104 74-75 0.9663976150715001 1104 76-77 1.2005823469134036 1104 78-79 1.1746307821985447 1104 80-81 0.9064228213151075 1104 82-83 1.1252356649376196 1104 84-85 1.1602489870455877 1104 86-87 1.104522413780522 1104 88-89 1.1676244416422676 1104 90-91 1.1684630282515123 1104 92-93 1.11673286028984 1104 94-95 1.0454298909013566 1104 96-97 1.029912941957896 1104 98-99 1.2545108356617334 1104 100-101 1.4648611096482824 1105 1 0.22505070913277692 1105 2 0.3266758223198494 1105 3 0.28867117590653635 1105 4 0.17519539270359985 1105 5 0.23434453652359366 1105 6 0.1705845028540125 1105 7 0.23083096966360728 1105 8 0.2287482955350626 1105 9 0.3158595077770201 1105 10-11 0.29907515024166287 1105 12-13 0.3013719466155109 1105 14-15 0.40490151456393875 1105 16-17 0.3261872452143919 1105 18-19 0.3953817905736443 1105 20-21 0.3236652534519422 1105 22-23 0.4484772198446123 1105 24-25 0.5530763472636622 1105 26-27 0.45102763418989156 1105 28-29 0.47703913209670645 1105 30-31 0.46861236678632423 1105 32-33 0.3857608317899235 1105 34-35 0.3584371712313086 1105 36-37 0.32964100974344746 1105 38-39 0.21434735951918782 1105 40-41 0.26521592495480917 1105 42-43 0.16221077398068218 1105 44-45 0.1862670828500299 1105 46-47 0.15001693600224542 1105 48-49 0.11319911716791609 1105 50-51 0.29037783478543133 1105 52-53 0.19605070618720077 1105 54-55 0.34713421541692213 1105 56-57 0.33821478268209404 1105 58-59 0.25885677160607656 1105 60-61 0.3742051451813495 1105 62-63 0.3802379625415213 1105 64-65 0.33392993360084233 1105 66-67 0.21133896273396857 1105 68-69 0.36555558920593967 1105 70-71 0.316110465466366 1105 72-73 0.3198832352368619 1105 74-75 0.43718845755012836 1105 76-77 0.4974771065785042 1105 78-79 0.4955335474675664 1105 80-81 0.44416710898314093 1105 82-83 0.43761037167678296 1105 84-85 0.5154817388607462 1105 86-87 0.5844951947271824 1105 88-89 0.4797338070869692 1105 90-91 0.4888762508406579 1105 92-93 0.4913186703568897 1105 94-95 0.38847971466688236 1105 96-97 0.2909982794719319 1105 98-99 0.5590571736538834 1105 100-101 0.912289954283974 1106 1 0.07885865399981995 1106 2 0.16489336344201178 1106 3 0.1409244754319232 1106 4 0.08196010241037754 1106 5 0.172300716225827 1106 6 0.1846850164747167 1106 7 0.13631591862661452 1106 8 0.22445442647583036 1106 9 0.3464268310980003 1106 10-11 0.18563077081250157 1106 12-13 0.21055804201460404 1106 14-15 0.2567178247301669 1106 16-17 0.2225730025340411 1106 18-19 0.24830049980364066 1106 20-21 0.20902853715694647 1106 22-23 0.3101527069173997 1106 24-25 0.20742404897355726 1106 26-27 0.2795206233756815 1106 28-29 0.3714181711708875 1106 30-31 0.25646517219559684 1106 32-33 0.2994942691477078 1106 34-35 0.2493872178255927 1106 36-37 0.3052626208220559 1106 38-39 0.28301263919325237 1106 40-41 0.23473962302384876 1106 42-43 0.21193324542813485 1106 44-45 0.20886106243522562 1106 46-47 0.10818289833672168 1106 48-49 0.10322291275988249 1106 50-51 0.027617762835191684 1106 52-53 -0.038634294788032264 1106 54-55 0.17879526909786847 1106 56-57 0.19534847576663594 1106 58-59 0.06569161112669519 1106 60-61 0.0038173073727989504 1106 62-63 0.04897198503680755 1106 64-65 0.02917471612472866 1106 66-67 0.018090582697993796 1106 68-69 0.021003002681439398 1106 70-71 0.0026576773944952947 1106 72-73 -0.01577231381716615 1106 74-75 0.12715391276543642 1106 76-77 0.12379451116857965 1106 78-79 0.09733993196792667 1106 80-81 0.22700281537414568 1106 82-83 0.29300737663961485 1106 84-85 0.1769135486693827 1106 86-87 0.206349690533699 1106 88-89 0.26539967189853186 1106 90-91 0.219195995980769 1106 92-93 0.31777585779593664 1106 94-95 0.24036715154788268 1106 96-97 0.18264169234062066 1106 98-99 0.08441148525044184 1106 100-101 0.5064144802142323 1107 1 0.1049730769154067 1107 2 0.15741041717671322 1107 3 0.24318257813846245 1107 4 -0.012825956156177654 1107 5 0.04813589993941747 1107 6 0.06843991284916484 1107 7 0.14319584108525163 1107 8 0.09392830523908913 1107 9 0.07196625207590301 1107 10-11 0.17674230210968744 1107 12-13 0.08124320652171235 1107 14-15 0.12360764427443627 1107 16-17 0.1819963993466942 1107 18-19 0.13521164663057306 1107 20-21 0.212587782968356 1107 22-23 0.31447301476616474 1107 24-25 0.21927333901523838 1107 26-27 0.2949079511881152 1107 28-29 0.34393093219375004 1107 30-31 0.19029310429238677 1107 32-33 0.314632253429906 1107 34-35 0.1667580510631126 1107 36-37 0.20805731250910497 1107 38-39 0.28944553192740585 1107 40-41 0.2343396513012337 1107 42-43 0.2263323977878926 1107 44-45 0.17870182326729633 1107 46-47 0.1731791549897892 1107 48-49 0.25470130057402685 1107 50-51 0.22671488799584694 1107 52-53 0.18035929425123953 1107 54-55 0.12142113115362463 1107 56-57 0.030503315756654104 1107 58-59 0.22207527395121218 1107 60-61 0.06776231807477018 1107 62-63 0.09153733177975454 1107 64-65 0.09713226256735652 1107 66-67 0.07995290418634227 1107 68-69 0.07077312114836332 1107 70-71 0.04625570240635568 1107 72-73 0.03380463261677491 1107 74-75 0.23664761976911564 1107 76-77 0.30743732006709834 1107 78-79 0.40504543490085254 1107 80-81 0.3906377543009505 1107 82-83 0.43233849718220085 1107 84-85 0.5218521092311157 1107 86-87 0.42115895018569915 1107 88-89 0.43383052389111043 1107 90-91 0.48416397524628607 1107 92-93 0.5479067344034689 1107 94-95 0.5255575086180393 1107 96-97 0.42980015558385176 1107 98-99 0.5321194411615622 1107 100-101 0.8500108017690131 1108 1 0.31501449796449066 1108 2 0.4156195997164538 1108 3 0.40454615208339817 1108 4 0.27549575481528166 1108 5 0.29231061237646827 1108 6 0.1775522939727523 1108 7 0.29693884082808353 1108 8 0.36116148402372517 1108 9 0.42681281442844465 1108 10-11 0.34108849119840556 1108 12-13 0.39768653908105733 1108 14-15 0.5079172060701964 1108 16-17 0.5543516566240996 1108 18-19 0.5267988542925863 1108 20-21 0.5138511374491443 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68-69 -0.6938802419740782 2316 70-71 -0.47286040143336905 2316 72-73 -0.48753992937782087 2316 74-75 -0.32528385333804977 2316 76-77 -0.8873736306156026 2316 78-79 -0.6916254432605093 2316 80-81 -0.6111317206262221 2316 82-83 -0.7428638138630674 2316 84-85 -0.8003093134775021 2316 86-87 -0.6944345818346065 2316 88-89 -0.7220093656343138 2316 90-91 -0.3545449377898322 2316 92-93 -0.6432622689955956 2316 94-95 -0.2154581684967205 2316 96-97 -0.21914926068108187 2316 98-99 -0.2971498993561994 2316 100-101 -0.46277885049892475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 27.0 4 55.0 5 126.0 6 234.0 7 627.0 8 1552.0 9 2739.0 10 4413.0 11 6571.0 12 9026.0 13 11378.0 14 13111.0 15 15805.0 16 18156.0 17 20513.0 18 19024.0 19 17296.0 20 18194.0 21 19870.0 22 22053.0 23 25809.0 24 31806.0 25 40133.0 26 50855.0 27 65130.0 28 81703.0 29 101228.0 30 122633.0 31 147064.0 32 176495.0 33 212887.0 34 259987.0 35 321072.0 36 410416.0 37 510979.0 38 407114.0 39 60500.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.415000291385766 20.371244068439697 13.70700354994662 24.506752090227913 2 15.89132811175512 21.644713454247242 38.994533676452036 23.4694247575456 3 18.516336348584808 26.102980146202405 30.635000430809246 24.745683074403544 4 13.04448653275639 17.507370191161474 38.01502592006865 31.433117356013483 5 12.901394220115387 38.18371720746869 35.48602448137088 13.428864091045037 6 31.029219383553723 38.001593632412664 17.726913637334263 13.24227334669935 7 26.884580732679954 32.54906956036909 22.992704688815728 17.573645018135228 8 25.563124943245093 36.01417966257288 20.681474236137003 17.741221158045033 9 26.150098927909077 15.563448988902223 20.3553160734298 37.9311360097589 10-11 24.6301284357835 26.203645765053107 29.16123911506657 20.004986684096817 12-13 25.178462235553106 23.738754779422134 29.021463196628766 22.061319788395995 14-15 22.27629372977645 25.39955863641245 26.24261546588714 26.081532167923953 16-17 22.286557368716785 28.262148340370167 27.023581840039945 22.427712450873102 18-19 22.263080593058994 27.3562702206321 28.587212579956628 21.793436606352273 20-21 22.82985489648059 26.685888179885097 28.464947391877438 22.01930953175687 22-23 22.6029472268345 26.558947248529215 28.38511525703974 22.452990267596544 24-25 22.505305947371944 26.74257911192326 28.349270804148556 22.40284413655624 26-27 22.42550421893523 26.896971199665277 28.180550282424587 22.496974298974905 28-29 22.502724624421777 27.078745481105994 28.160862631665644 22.25766726280659 30-31 22.73673244929229 26.819415759284038 28.084960180339536 22.358891611084136 32-33 22.52946298732077 26.951011322057877 27.978902774389326 22.540622916232024 34-35 22.726623908700898 26.93643354674087 28.063010836312767 22.273931708245463 36-37 22.83535191168205 26.746223814487802 27.98885937525908 22.429564898571066 38-39 22.631711073988786 26.900873063345905 27.987987243419482 22.479428619245827 40-41 22.726553544666515 26.806929392736716 28.06303640474177 22.403480657854995 42-43 22.708475523990003 26.903433094608005 27.681801021727043 22.706290359674945 44-45 22.564121717549686 26.954809456203076 27.962479064676298 22.518589761570937 46-47 22.798345975731433 27.00588876528719 27.542786456242773 22.652978802738605 48-49 22.710970362777555 27.13699540053815 27.43732888399431 22.714705352689986 50-51 22.74889764435957 27.092077182204644 27.49355616987711 22.66546900355868 52-53 22.9468625248766 26.99541507722057 27.437364298875195 22.620358099027634 54-55 22.572616526031222 26.957909802157687 27.6737757803663 22.795697891444792 56-57 22.65696593611559 27.137414015019136 27.402972676206733 22.802647372658537 58-59 22.65433906102729 27.04100730452638 27.568011360633303 22.736642273813025 60-61 22.654232084329575 27.19505424095303 27.585726784737215 22.56498688998018 62-63 22.657200430655728 27.09391300921852 27.56115989156361 22.687726668562146 64-65 22.438144592961848 26.98464479066275 27.526728401467626 23.05048221490778 66-67 22.44117042523899 27.129867561226273 27.44151558046189 22.98744643307285 68-69 22.548740386646898 27.03638345260732 27.550228015131307 22.864648145614474 70-71 22.492159642232444 26.87201095812049 27.48723416499007 23.148595234656995 72-73 22.472796463113756 27.100693017565025 27.32981216107037 23.096698358250848 74-75 22.50612079941065 27.2238930412145 27.2549105795589 23.015075579815957 76-77 22.473863832896352 26.970905289793336 27.312560728255807 23.242670149054508 78-79 22.394598462251583 27.055203875722356 27.342634790097325 23.20756287192874 80-81 22.522617734485724 27.18264120839494 27.149401483499 23.145339573620337 82-83 22.580102293657735 27.029408419345113 27.26135586359978 23.12913342339737 84-85 22.498917588924293 27.102039209779477 27.08877444412729 23.310268757168938 86-87 22.389142355419477 27.17764527475266 27.11406427770717 23.3191480921207 88-89 22.34546724955251 27.037077384650267 27.100193563513425 23.517261802283805 90-91 22.351442312237193 27.033554330268633 27.159129106460227 23.455874251033944 92-93 22.414763145886855 26.8504615071768 27.272708955371932 23.462066391564413 94-95 22.22608774347554 26.78389372113482 27.31423118423954 23.6757873511501 96-97 22.17431823598452 26.590911837966548 27.38219539929579 23.85257452675314 98-99 21.990269612560372 26.692319111830965 27.237584659732416 24.07982661587625 100-101 22.043576614714034 26.618681066995435 27.21852430721817 24.11921801107236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 395.0 1 292.0 2 333.5 3 454.5 4 346.5 5 303.0 6 441.0 7 777.0 8 1176.5 9 1340.0 10 1192.5 11 1003.0 12 1038.5 13 1201.0 14 1414.5 15 1594.0 16 1771.0 17 1997.0 18 2266.5 19 2590.5 20 2993.5 21 3554.5 22 4365.0 23 5516.5 24 7224.5 25 9673.5 26 12716.5 27 16468.5 28 21017.0 29 27056.5 30 34660.5 31 43402.5 32 54200.5 33 61719.5 34 66278.5 35 71922.0 36 79650.0 37 88929.0 38 95516.0 39 99732.0 40 102815.5 41 106296.5 42 110103.5 43 113795.0 44 117044.5 45 120784.5 46 123920.0 47 125172.0 48 126128.0 49 125410.5 50 122480.0 51 118988.5 52 116596.5 53 115112.0 54 111259.0 55 102741.0 56 92466.0 57 81421.5 58 70804.5 59 60893.0 60 50496.0 61 41701.5 62 34446.0 63 26535.5 64 19577.0 65 14811.5 66 10964.5 67 8536.0 68 6607.5 69 5023.0 70 3903.0 71 2930.5 72 2216.5 73 1724.5 74 1339.0 75 1044.0 76 794.5 77 496.5 78 313.0 79 219.5 80 141.0 81 80.5 82 46.5 83 33.5 84 23.0 85 12.5 86 7.0 87 3.5 88 2.5 89 2.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019494246717822794 2 0.0034091687423855443 3 0.003316191413047757 4 0.03409168742385544 5 0.0017975617005305596 6 6.198488622519171E-5 7 0.0 8 0.0 9 3.0992443112595855E-5 10-11 0.0 12-13 0.0 14-15 4.648866466889378E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5496221556297928E-5 24-25 7.748110778148964E-4 26-27 0.002107486131656518 28-29 4.648866466889378E-5 30-31 0.007267727909903728 32-33 0.023740211424248425 34-35 0.013931103179111838 36-37 7.283224131460025E-4 38-39 7.128261915897047E-4 40-41 0.002370921898113583 42-43 0.009018800945765394 44-45 0.008910327394871308 46-47 0.00867788407152684 48-49 0.010723385316958167 50-51 0.18212709195116955 52-53 0.05191234221359806 54-55 0.019835163592061347 56-57 0.011637662388779743 58-59 0.02220608549017493 60-61 0.05484112808773837 62-63 0.0972077978226569 64-65 0.10194964161888408 66-67 0.1055447650199452 68-69 0.10357674488229536 70-71 0.09444947038563586 72-73 0.07091070984161932 74-75 0.23078522763794507 76-77 0.2751354137320697 78-79 0.007825591885930454 80-81 9.917581796030674E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0847355089408549E-4 90-91 0.007081773251228152 92-93 0.019866156035173943 94-95 0.0019370276945372409 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3226593.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.67007803976807 #Duplication Level Percentage of deduplicated Percentage of total 1 79.13396527239827 46.42795918127903 2 11.630242441496245 13.64694463328015 3 3.6575325178394533 6.437631547638903 4 1.6599246600816262 3.8955163738849796 5 0.9656860529233319 2.8328438043463735 6 0.5972710163603728 2.1025162284452796 7 0.43208241879909787 1.7745216461388373 8 0.30068885012497953 1.4113150642016539 9 0.2395523573971838 1.2649099952792866 >10 1.2866409111033177 13.411499620578777 >50 0.06988080160556512 2.7874510140054642 >100 0.02393908997062161 2.441803678818865 >500 0.001693786058573611 0.6605302797446853 >1k 8.998238414095128E-4 0.9045569323578501 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.13642873458164695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.11110790855865613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.198488622519171E-5 2 0.0 0.0 0.0 0.0 9.297732933778757E-5 3 0.0 0.0 0.0 0.0 9.297732933778757E-5 4 0.0 0.0 0.0 0.0 9.297732933778757E-5 5 0.0 0.0 0.0 0.0 9.297732933778757E-5 6 0.0 0.0 0.0 0.0 9.297732933778757E-5 7 0.0 0.0 0.0 0.0 9.297732933778757E-5 8 0.0 0.0 0.0 0.0 9.297732933778757E-5 9 0.0 0.0 0.0 0.0 9.297732933778757E-5 10-11 0.0 0.0 0.0 1.5496221556297928E-5 9.297732933778757E-5 12-13 0.0 0.0 0.0 3.0992443112595855E-5 3.254206526822565E-4 14-15 0.0 1.5496221556297928E-5 0.0 6.198488622519171E-5 3.5641309579485233E-4 16-17 0.0 3.0992443112595855E-5 0.0 6.198488622519171E-5 3.7190931735115026E-4 18-19 0.0 3.0992443112595855E-5 0.0 7.748110778148964E-5 3.7190931735115026E-4 20-21 0.0 3.0992443112595855E-5 0.0 1.704584371192772E-4 4.8038286824523575E-4 22-23 1.5496221556297928E-5 3.0992443112595855E-5 0.0 4.1839798202004404E-4 5.578639760267254E-4 24-25 3.0992443112595855E-5 3.0992443112595855E-5 0.0 5.888564191393212E-4 5.578639760267254E-4 26-27 3.0992443112595855E-5 3.0992443112595855E-5 0.0 0.0011622166167223446 6.973299700334067E-4 28-29 3.0992443112595855E-5 3.0992443112595855E-5 0.0 0.0025723727783454556 7.438186347023005E-4 30-31 3.0992443112595855E-5 3.0992443112595855E-5 0.0 0.0082284936463942 7.593148562585984E-4 32-33 3.0992443112595855E-5 3.0992443112595855E-5 0.0 0.021725702621929695 7.748110778148964E-4 34-35 3.0992443112595855E-5 3.0992443112595855E-5 0.0 0.040522619369719085 7.748110778148964E-4 36-37 6.198488622519171E-5 3.0992443112595855E-5 0.0 0.07105017583562599 7.748110778148964E-4 38-39 6.198488622519171E-5 3.0992443112595855E-5 0.0 0.1158962410195522 7.748110778148964E-4 40-41 6.198488622519171E-5 3.0992443112595855E-5 0.0 0.16715774192778576 7.748110778148964E-4 42-43 6.198488622519171E-5 3.0992443112595855E-5 0.0 0.22102260805747737 7.903072993711944E-4 44-45 6.198488622519171E-5 3.0992443112595855E-5 0.0 0.28469658243230556 8.212997424837901E-4 46-47 6.198488622519171E-5 9.297732933778757E-5 0.0 0.3583811159325022 8.987808502652798E-4 48-49 6.198488622519171E-5 9.297732933778757E-5 0.0 0.4381711607258802 9.607657364904715E-4 50-51 6.198488622519171E-5 9.297732933778757E-5 0.0 0.5259107671776391 9.917581796030674E-4 52-53 6.198488622519171E-5 9.297732933778757E-5 0.0 0.6139912905036364 0.001084735508940855 54-55 6.198488622519171E-5 9.297732933778757E-5 0.0 0.7105172545778162 0.001084735508940855 56-57 6.198488622519171E-5 9.297732933778757E-5 0.0 0.8201685183101803 0.0011157279520534508 58-59 6.198488622519171E-5 9.297732933778757E-5 0.0 0.9462922655568893 0.0011932090598349406 60-61 9.297732933778757E-5 9.297732933778757E-5 0.0 1.084689020276186 0.001255193946060132 62-63 9.297732933778757E-5 9.297732933778757E-5 0.0 1.2270683039354515 0.0014411486047357074 64-65 9.297732933778757E-5 9.297732933778757E-5 0.0 1.3759869930914745 0.0014721410478483032 66-67 9.297732933778757E-5 9.297732933778757E-5 0.0 1.5435786292228366 0.001549622155629793 68-69 9.297732933778757E-5 9.297732933778757E-5 0.0 1.730323595197783 0.0015806145987423887 70-71 9.297732933778757E-5 9.297732933778757E-5 0.0 1.9364233418965453 0.0016425994849675802 72-73 1.2396977245038342E-4 9.297732933778757E-5 0.0 2.1549510582834586 0.0017975617005305596 74-75 1.2396977245038342E-4 9.297732933778757E-5 0.0 2.383504830017297 0.001937027694537241 76-77 1.2396977245038342E-4 9.297732933778757E-5 0.0 2.631568344690514 0.001968020137649837 78-79 1.2396977245038342E-4 9.297732933778757E-5 0.0 2.911027204236791 0.0019835163592061347 80-81 1.2396977245038342E-4 9.297732933778757E-5 0.0 3.2194949905364574 0.0020300050238750283 82-83 1.2396977245038342E-4 9.297732933778757E-5 0.0 3.5515015373801404 0.0020764936885439224 84-85 1.2396977245038342E-4 9.297732933778757E-5 0.0 3.89641643678022 0.0020764936885439224 86-87 1.2396977245038342E-4 9.297732933778757E-5 0.0 4.259213975856268 0.00216947101788171 88-89 1.2396977245038342E-4 9.297732933778757E-5 0.0 4.654429610428089 0.0022314559041069016 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3140 0.0 54.900215 1 GTATCAA 5960 0.0 40.158855 1 TCAACGC 7370 0.0 31.43753 4 ATCAACG 7425 0.0 31.206593 3 CAACGCA 7450 0.0 31.154497 5 TATCAAC 7650 0.0 30.538439 2 AACGCAG 7815 0.0 29.940973 6 GTACATG 8930 0.0 27.919325 1 TACATGG 9195 0.0 26.285076 2 ACATGGG 9120 0.0 25.666998 3 ACGCAGA 9285 0.0 25.098099 7 CGCAGAG 9560 0.0 24.376135 8 CATGGGG 6745 0.0 21.891449 4 GCAGAGT 11150 0.0 20.516977 9 AGAGTAC 10225 0.0 19.402346 10-11 ATGGGGG 4090 0.0 18.800087 5 CCGTCGA 180 1.2418964E-4 18.457241 9 TATACTG 1645 0.0 18.177862 5 CAGAGTA 10660 0.0 18.009537 10-11 GTACTAG 720 0.0 17.148964 1 >>END_MODULE