FastQCFastQC Report
Sat 18 Jun 2016
SRR1930968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194020
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57230.17917858999004388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.052.806331
GTATCAA49700.049.4226381
ATCAACG62150.038.2040563
TATCAAC63050.038.0711562
TCAACGC64100.037.5025334
CAACGCA65750.036.305395
AACGCAG71300.033.5882456
ACGCAGA83000.028.3791547
CGCAGAG85550.027.9851688
GTACATG67600.026.4328611
TACATGG69300.024.9071272
GCAGAGT93700.024.0230039
ACATGGG72800.023.3557553
AGAGTAC82650.020.56531710-11
GAGTACA51650.020.3675351
TATACGG4700.020.3431642
GAGTACT56050.020.20814912-13
GTATACG4500.020.133651
CAGAGTA90950.019.71057710-11
CATGGGG58200.019.701654
Produced by FastQC (version 0.11.5)