FastQCFastQC Report
Sat 18 Jun 2016
SRR1930968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194020
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.1652776125384312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44060.13794528525181432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.054.341831
GTATCAA68300.043.0343551
ATCAACG85500.034.0354043
CAACGCA85800.033.5718045
TCAACGC86750.033.4886864
TATCAAC88050.033.3198052
AACGCAG89500.032.4485176
ACGCAGA104550.027.4598317
CGCAGAG108000.026.5387028
GTACATG90700.024.9721781
TACATGG93500.024.066342
GCAGAGT122450.022.7481389
ACATGGG96950.022.6709483
GTACTAG7100.020.7323571
CATGGGG70850.020.5690694
AGAGTAC113050.019.91737710-11
GTGGTAT17700.019.851981
CAGAGTA119000.019.53959810-11
GAGTACT76850.019.3888612-13
GAGTACA71250.018.1271441
Produced by FastQC (version 0.11.5)