##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930968_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3194020 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24696057006531 31.0 31.0 33.0 30.0 34.0 2 31.367125440667248 31.0 31.0 34.0 30.0 34.0 3 31.448621173317637 31.0 31.0 34.0 30.0 34.0 4 35.319703070112276 37.0 35.0 37.0 33.0 37.0 5 35.10449151852524 37.0 35.0 37.0 32.0 37.0 6 35.1447245164401 37.0 35.0 37.0 32.0 37.0 7 35.026506095766464 36.0 35.0 37.0 32.0 37.0 8 35.002571680828545 36.0 35.0 37.0 32.0 37.0 9 36.50723696157194 38.0 35.0 39.0 32.0 39.0 10-11 36.431231019217165 38.0 35.0 39.0 32.0 39.0 12-13 36.324039454981495 38.0 35.0 39.0 32.0 39.0 14-15 37.33923300417655 39.0 36.0 41.0 32.0 41.0 16-17 37.303177187368895 39.0 36.0 40.5 32.0 41.0 18-19 37.29044808736326 39.0 36.0 40.0 32.0 41.0 20-21 37.25121132616577 39.0 36.0 40.0 31.0 41.0 22-23 37.12505087632513 39.0 36.0 40.0 31.0 41.0 24-25 37.00222274750941 39.0 36.0 40.0 31.0 41.0 26-27 36.7247559501819 38.5 35.5 40.0 30.0 41.0 28-29 36.530575105979295 38.0 35.0 40.0 30.0 41.0 30-31 36.28529595932399 38.0 35.0 40.0 30.0 41.0 32-33 36.04498140274639 38.0 34.5 40.0 29.5 41.0 34-35 36.23891569245026 38.0 35.0 40.0 30.0 41.0 36-37 36.29252712882199 38.0 35.0 40.0 30.0 41.0 38-39 36.24986599958673 38.0 35.0 40.0 30.0 41.0 40-41 36.1462005560391 38.0 35.0 40.0 30.0 41.0 42-43 35.993172240624666 38.0 35.0 40.0 29.0 41.0 44-45 35.80280571192416 38.0 34.5 40.0 28.5 41.0 46-47 35.572892780884274 38.0 34.0 40.0 28.0 41.0 48-49 35.361534054263906 38.0 34.0 40.0 27.0 41.0 50-51 35.082897258000884 38.0 34.0 40.0 26.5 41.0 52-53 34.859951252653396 38.0 33.0 40.0 26.0 41.0 54-55 34.67696226072473 38.0 33.0 40.0 26.0 41.0 56-57 34.41102763915066 37.0 33.0 40.0 26.0 41.0 58-59 34.15327486991315 37.0 33.0 40.0 25.0 41.0 60-61 33.832798949286484 36.5 32.0 39.5 24.5 41.0 62-63 33.52140813144564 36.0 32.0 39.0 23.5 41.0 64-65 33.20644031659163 36.0 31.5 39.0 23.0 40.0 66-67 32.8637716733145 35.0 31.0 39.0 22.5 40.0 68-69 32.48536108102016 35.0 31.0 38.0 22.0 40.0 70-71 32.081798329378024 35.0 31.0 38.0 21.5 40.0 72-73 31.658532037996004 34.5 30.0 37.0 20.5 39.5 74-75 31.128725712425094 34.0 30.0 36.5 19.0 39.0 76-77 29.77725327330449 32.5 28.5 35.0 18.0 37.5 78-79 30.538245846926444 34.0 29.5 36.0 18.5 38.0 80-81 30.543743151263925 34.0 30.0 35.0 18.5 37.0 82-83 30.27776375852374 34.0 30.0 35.0 18.0 37.0 84-85 29.959955322759406 34.0 29.0 35.0 17.5 36.5 86-87 29.622769894991265 34.0 29.0 35.0 13.0 36.0 88-89 29.281924033036738 34.0 29.0 35.0 8.5 36.0 90-91 28.940278238708586 34.0 29.0 35.0 4.5 35.0 92-93 28.58035907727566 33.0 29.0 35.0 2.0 35.0 94-95 28.16242665982054 33.0 28.0 35.0 2.0 35.0 96-97 27.686650521912824 33.0 27.0 35.0 2.0 35.0 98-99 27.189197155935155 33.0 27.0 35.0 2.0 35.0 100-101 25.965882492908623 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48794005136252494 1101 2 0.570302604508754 1101 3 0.5473423738770045 1101 4 0.38797429781471493 1101 5 0.516840939148338 1101 6 0.4931756835754655 1101 7 0.4854599748347752 1101 8 0.49364407522612197 1101 9 0.6197379500096858 1101 10-11 0.5285876608604312 1101 12-13 0.5478944534235595 1101 14-15 0.7289833973409969 1101 16-17 0.7260574523753789 1101 18-19 0.705608652546438 1101 20-21 0.7517248437694874 1101 22-23 0.654151285309652 1101 24-25 0.6685680890815036 1101 26-27 0.5592737599515729 1101 28-29 0.5669444442044593 1101 30-31 0.7487832957068363 1101 32-33 0.631789336948728 1101 34-35 0.5873989283500123 1101 36-37 0.6645758406630264 1101 38-39 0.6759775450631125 1101 40-41 0.8185936685800641 1101 42-43 0.7830503595118543 1101 44-45 0.7646724387248653 1101 46-47 0.6541763794520676 1101 48-49 0.6346285706146872 1101 50-51 0.7189444779536913 1101 52-53 0.598806464365488 1101 54-55 0.6252248534352347 1101 56-57 0.8668426684460329 1101 58-59 0.8280067296799132 1101 60-61 0.8245534951825206 1101 62-63 0.6128290649420478 1101 64-65 0.6482879592828397 1101 66-67 0.8026382820503741 1101 68-69 0.8431488864254533 1101 70-71 0.6374556593412919 1101 72-73 0.5708561890515647 1101 74-75 0.6152226708063075 1101 76-77 0.7115422572184187 1101 78-79 0.7595146618443351 1101 80-81 0.6404907083824725 1101 82-83 0.7364718850517242 1101 84-85 0.9262568862603153 1101 86-87 0.9401912795668963 1101 88-89 0.8302321027866988 1101 90-91 0.731600405914488 1101 92-93 0.922874729311129 1101 94-95 0.8547676872050509 1101 96-97 0.8960518962588715 1101 98-99 0.9175339331659451 1101 100-101 1.1186359874445024 1102 1 0.4252077473057021 1102 2 0.49138400177328023 1102 3 0.5463416614050622 1102 4 0.29459874559257315 1102 5 0.4233901079357736 1102 6 0.4182971473072996 1102 7 0.4827854560963658 1102 8 0.42360876928563584 1102 9 0.64869038130022 1102 10-11 0.640329172167462 1102 12-13 0.5602306217642976 1102 14-15 0.7682365508117357 1102 16-17 0.6979858134671701 1102 18-19 0.7313571216621781 1102 20-21 0.6348887962416612 1102 22-23 0.7530144503786147 1102 24-25 0.7669814128425259 1102 26-27 0.704941895764648 1102 28-29 0.7531398844404151 1102 30-31 0.7856893141634416 1102 32-33 0.7771306059344667 1102 34-35 0.7575398316358672 1102 36-37 0.7164752066560354 1102 38-39 0.7931294636106827 1102 40-41 0.815306439007621 1102 42-43 0.6965813022313085 1102 44-45 0.799482487088568 1102 46-47 0.8596797059658101 1102 48-49 0.7417891532947536 1102 50-51 0.870855976927551 1102 52-53 0.9728090764806652 1102 54-55 0.9264769469853107 1102 56-57 0.6845021046016981 1102 58-59 0.83147557804012 1102 60-61 0.8644115590426011 1102 62-63 0.656862481295299 1102 64-65 0.6463177361177515 1102 66-67 0.6007692579076291 1102 68-69 0.6341232362137532 1102 70-71 0.6994428329666889 1102 72-73 0.5830378991891045 1102 74-75 0.6890887469448508 1102 76-77 0.8248264709321802 1102 78-79 0.7324342433006699 1102 80-81 0.6142952643872661 1102 82-83 0.782705355744941 1102 84-85 0.8129232853936585 1102 86-87 0.7697049481545726 1102 88-89 0.7803701565607106 1102 90-91 0.7175686276546251 1102 92-93 0.8639209418709939 1102 94-95 0.8324852470116717 1102 96-97 0.8500922399709019 1102 98-99 0.9706745566695894 1102 100-101 1.2657618666039596 1103 1 0.4115005562078018 1103 2 0.46600756810178723 1103 3 0.4499616704939129 1103 4 0.4304577117611075 1103 5 0.34834358427584533 1103 6 0.415382420803482 1103 7 0.4651359199425613 1103 8 0.35994096687818455 1103 9 0.5039512138304119 1103 10-11 0.5158403286984523 1103 12-13 0.4996718365678632 1103 14-15 0.589877681605607 1103 16-17 0.5453102730938539 1103 18-19 0.5590570764374476 1103 20-21 0.6211384751680384 1103 22-23 0.63501897797412 1103 24-25 0.6276961977433189 1103 26-27 0.5700106585755762 1103 28-29 0.5912445913980662 1103 30-31 0.6710979539894524 1103 32-33 0.6583202834265265 1103 34-35 0.6211080097451926 1103 36-37 0.6842561543117043 1103 38-39 0.6202642007401096 1103 40-41 0.6654538767486073 1103 42-43 0.6423633095214214 1103 44-45 0.6472459302857914 1103 46-47 0.6478830310079431 1103 48-49 0.6085414944794749 1103 50-51 0.6849860887672818 1103 52-53 0.6417469502890398 1103 54-55 0.7128705053153297 1103 56-57 0.6390731593843597 1103 58-59 0.8025441000915592 1103 60-61 0.7520037739493475 1103 62-63 0.6792207314056142 1103 64-65 0.5908676805381674 1103 66-67 0.44818477986905947 1103 68-69 0.608691872424707 1103 70-71 0.46646490343970015 1103 72-73 0.5038431225721887 1103 74-75 0.7736085891120119 1103 76-77 0.6916499788637083 1103 78-79 0.8394098925645928 1103 80-81 0.8172345150617986 1103 82-83 0.7875773049966455 1103 84-85 0.7927628345660374 1103 86-87 0.8528026902843315 1103 88-89 0.7651503061974871 1103 90-91 0.8356730455545467 1103 92-93 0.8757819138806902 1103 94-95 0.925188944901695 1103 96-97 0.8953597336354129 1103 98-99 0.8987901508588685 1103 100-101 1.0388768351112994 1104 1 0.4490251696652372 1104 2 0.5041890953733912 1104 3 0.3530073426962872 1104 4 0.3009378257698714 1104 5 0.42044147451086644 1104 6 0.379587145886326 1104 7 0.4487357690898435 1104 8 0.4205271369365846 1104 9 0.4992723787050011 1104 10-11 0.4647835710645083 1104 12-13 0.560367010332385 1104 14-15 0.7249111923591229 1104 16-17 0.6065174313076511 1104 18-19 0.6122414654944563 1104 20-21 0.57345649505789 1104 22-23 0.6153768430997388 1104 24-25 0.6550932803695346 1104 26-27 0.7852766801926592 1104 28-29 0.6234899731108214 1104 30-31 0.6010948801550384 1104 32-33 0.8205187620011642 1104 34-35 0.7616462514698483 1104 36-37 0.7807788484627309 1104 38-39 0.8041446468071101 1104 40-41 0.705621129091135 1104 42-43 0.7153266576385988 1104 44-45 0.7802294879003853 1104 46-47 0.8670730546102234 1104 48-49 0.7387964544985977 1104 50-51 0.7435048685987269 1104 52-53 0.7615419356979558 1104 54-55 0.7647682738587562 1104 56-57 0.8721995346361027 1104 58-59 0.7581239507389625 1104 60-61 0.6561718163058785 1104 62-63 0.6607172646671629 1104 64-65 0.7490341610923963 1104 66-67 0.7636310343825485 1104 68-69 0.6409662137233738 1104 70-71 0.6296700452601769 1104 72-73 0.6879660668358945 1104 74-75 0.8350539957799548 1104 76-77 0.8911820515393885 1104 78-79 0.9154904925686616 1104 80-81 0.8592319168480529 1104 82-83 0.9134204626833018 1104 84-85 0.9377194942017901 1104 86-87 0.937570552697121 1104 88-89 1.0858865701532885 1104 90-91 0.9722913159776674 1104 92-93 0.9501149934562001 1104 94-95 0.9143369863048818 1104 96-97 1.0079967067812454 1104 98-99 1.1717352119394988 1104 100-101 1.5448162665535783 1105 1 0.17363965340312504 1105 2 0.22698916234977773 1105 3 0.2736299203600403 1105 4 0.16044918195652969 1105 5 0.15985130660636315 1105 6 0.24336398965255057 1105 7 0.24956521675224508 1105 8 0.2151811348497219 1105 9 0.3147004079437892 1105 10-11 0.36898085246654944 1105 12-13 0.2146856154829493 1105 14-15 0.3034381659630512 1105 16-17 0.2852062645090143 1105 18-19 0.2183909950042704 1105 20-21 0.2536910826848455 1105 22-23 0.3543193540898244 1105 24-25 0.36530311132983684 1105 26-27 0.3712074769373075 1105 28-29 0.43783483905872345 1105 30-31 0.3635723063698606 1105 32-33 0.46397593542743465 1105 34-35 0.4343133589697814 1105 36-37 0.4693741323035425 1105 38-39 0.4046314553918151 1105 40-41 0.3567678158885599 1105 42-43 0.25148633766553985 1105 44-45 0.2190023821410776 1105 46-47 0.32151019282659377 1105 48-49 0.45424330902078225 1105 50-51 0.3801754800609629 1105 52-53 0.4133616973263585 1105 54-55 0.4734044435663165 1105 56-57 0.3666508060049267 1105 58-59 0.18897414405286383 1105 60-61 0.2597742565923582 1105 62-63 0.24987153644560323 1105 64-65 0.3342507322609549 1105 66-67 0.42330349337134265 1105 68-69 0.39450210332207547 1105 70-71 0.3095068423510625 1105 72-73 0.29878360443207086 1105 74-75 0.5097209348929326 1105 76-77 0.4381610665513662 1105 78-79 0.5386471047366044 1105 80-81 0.47088145629080813 1105 82-83 0.5777789036471148 1105 84-85 0.6077326151405202 1105 86-87 0.5503812999473539 1105 88-89 0.6420450393077317 1105 90-91 0.4048348388030867 1105 92-93 0.39951376688019735 1105 94-95 0.2856103606376017 1105 96-97 0.316737503440752 1105 98-99 0.5268656692614435 1105 100-101 0.8963940428808321 1106 1 0.08867512943087519 1106 2 0.16124646276680465 1106 3 0.13984375726415976 1106 4 0.06277895566740455 1106 5 0.12407397408438214 1106 6 0.19669049443762532 1106 7 0.09130698076764787 1106 8 0.1214904071747398 1106 9 0.05795067201321302 1106 10-11 0.09044644781594258 1106 12-13 0.08782582631587132 1106 14-15 0.19677884285166414 1106 16-17 0.19714181745417392 1106 18-19 0.34754453305546207 1106 20-21 0.22439740626102633 1106 22-23 0.25369690565233327 1106 24-25 0.14725461323831723 1106 26-27 0.3025330442727139 1106 28-29 0.18806678470477323 1106 30-31 0.1346287577268086 1106 32-33 0.2314733631726753 1106 34-35 0.12279617651332586 1106 36-37 0.287656684832335 1106 38-39 0.21346046373552952 1106 40-41 0.17321931313460226 1106 42-43 0.2202128356579962 1106 44-45 0.2575087813599737 1106 46-47 0.0832779108005326 1106 48-49 0.10853303948133686 1106 50-51 0.09204988233842215 1106 52-53 0.12031910855544226 1106 54-55 0.27305849914823455 1106 56-57 0.2917649780143634 1106 58-59 0.324154290857237 1106 60-61 0.09574721478842463 1106 62-63 0.11855864752227063 1106 64-65 0.05188752415730846 1106 66-67 0.0057325915760841895 1106 68-69 0.09372953290406372 1106 70-71 0.05898650988237364 1106 72-73 0.11382931216079228 1106 74-75 0.24170666081457526 1106 76-77 0.1406167432248253 1106 78-79 0.1770627275516219 1106 80-81 0.12077805708191747 1106 82-83 0.10807564607371134 1106 84-85 0.14637234300464996 1106 86-87 0.24758420441586182 1106 88-89 0.28855003654406275 1106 90-91 0.23523934773234245 1106 92-93 0.32362320467474603 1106 94-95 0.36554839040723053 1106 96-97 0.28790921542247716 1106 98-99 0.3663003386958046 1106 100-101 0.5824166325260585 1107 1 0.08029962368236099 1107 2 0.12800882556449267 1107 3 0.09166617232168761 1107 4 3.1644194633173583E-4 1107 5 0.049266372727217345 1107 6 0.1404710274123957 1107 7 0.13774493250049602 1107 8 0.12816026614874687 1107 9 0.1757346687370216 1107 10-11 0.09072091147121597 1107 12-13 0.046778350892360265 1107 14-15 0.08624508883990956 1107 16-17 0.055686774676807715 1107 18-19 0.08477942199487387 1107 20-21 0.1512925421696636 1107 22-23 0.19593776034961508 1107 24-25 0.1933224670295175 1107 26-27 0.15216094762508448 1107 28-29 0.20828390658165574 1107 30-31 0.20216347280409508 1107 32-33 0.20658584730953322 1107 34-35 0.031002947623200328 1107 36-37 0.00865858132601005 1107 38-39 0.12525997210445894 1107 40-41 0.06122009440093734 1107 42-43 0.2040753088314844 1107 44-45 0.1746016559141026 1107 46-47 0.30538301709795945 1107 48-49 0.2665766672693195 1107 50-51 0.2503115546127077 1107 52-53 0.0730274479326809 1107 54-55 0.2467187167693723 1107 56-57 0.1880575217784326 1107 58-59 0.2685988664872596 1107 60-61 0.1657569941110424 1107 62-63 0.009694867178204447 1107 64-65 0.049576235735770524 1107 66-67 0.1889155131478688 1107 68-69 0.25953627810665836 1107 70-71 0.050603624945090075 1107 72-73 0.1516596721167751 1107 74-75 0.2636264356236637 1107 76-77 0.39472290335474014 1107 78-79 0.3771553342622802 1107 80-81 0.38350273018807 1107 82-83 0.3667640715926481 1107 84-85 0.44985282382753056 1107 86-87 0.25426296058317277 1107 88-89 0.3278933831976012 1107 90-91 0.3655388281025189 1107 92-93 0.45939344692803274 1107 94-95 0.3748427949075399 1107 96-97 0.43692033666111385 1107 98-99 0.4736244829291749 1107 100-101 0.8186595054947183 1108 1 0.2557380078568272 1108 2 0.3353321202144315 1108 3 0.2683965787530269 1108 4 0.1957448649360103 1108 5 0.204810511821087 1108 6 0.19268781715639705 1108 7 0.30186465626000114 1108 8 0.33865446977554114 1108 9 0.2485246118266744 1108 10-11 0.2887169730191985 1108 12-13 0.2781363702597446 1108 14-15 0.35810165664243954 1108 16-17 0.4770266102797578 1108 18-19 0.35578914420668895 1108 20-21 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-0.7155874208227644 2316 62-63 -1.073672323181107 2316 64-65 -0.9411663159983163 2316 66-67 -0.5925320677816117 2316 68-69 -0.8017568308413345 2316 70-71 -0.49227468954582676 2316 72-73 -0.5516667387138874 2316 74-75 -0.23403638029596507 2316 76-77 -0.7498872399863927 2316 78-79 -0.6467245717886705 2316 80-81 -0.7508212429367234 2316 82-83 -0.9284029498732664 2316 84-85 -0.817467143535417 2316 86-87 -0.6204065136983559 2316 88-89 -0.8392319622280056 2316 90-91 -0.7287424264094184 2316 92-93 -0.9331367977491389 2316 94-95 -0.5794707860689527 2316 96-97 -0.4924004634167858 2316 98-99 -0.3826228518793151 2316 100-101 -0.4703716994908298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 27.0 4 57.0 5 109.0 6 262.0 7 615.0 8 1479.0 9 2564.0 10 4190.0 11 6327.0 12 8887.0 13 11599.0 14 13480.0 15 16623.0 16 19268.0 17 21832.0 18 19904.0 19 17876.0 20 18274.0 21 19544.0 22 21510.0 23 25233.0 24 30864.0 25 38549.0 26 49314.0 27 63021.0 28 79193.0 29 98231.0 30 120105.0 31 144889.0 32 173077.0 33 209302.0 34 258555.0 35 319705.0 36 410068.0 37 507117.0 38 399784.0 39 62570.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.62546482574535 20.85353514951614 14.464453415026801 24.056546609711717 2 15.640816188513234 21.450262201389577 39.43869584670118 23.470225763396 3 18.655530855693666 25.916716240333137 30.438429506246283 24.989323397726917 4 13.242321460449297 17.680105481069468 37.941772445262906 31.135800613218333 5 13.300498317442862 38.0252590211237 35.20758634768005 13.466656313753386 6 31.13977441579853 37.94330526628216 17.47930036712379 13.437619950795519 7 26.78286297518488 32.62703427029261 22.748761748517545 17.84134100600497 8 25.292859781717087 36.26599082034552 20.48487485989443 17.95627453804297 9 25.89287223803999 15.790581017389382 20.62233212211077 37.69421462245986 10-11 24.633173868667072 26.450304005610487 28.73339866375289 20.183123461969554 12-13 25.133421205878488 23.806284869850533 28.86336027952236 22.196933644748622 14-15 22.281184839168194 25.51325602219147 26.193104614247876 26.01245452439246 16-17 22.19438200136505 28.379722105685 26.997451487467206 22.428444405482743 18-19 22.122466359008396 27.53420454474299 28.755972097857875 21.587356998390742 20-21 22.73706802086399 26.640143142497543 28.750101752650263 21.872687083988204 22-23 22.53081815274395 26.537510662508684 28.578497588539324 22.353173596208038 24-25 22.405466876247164 26.75878628033599 28.591206729994788 22.244540113422058 26-27 22.38945646254105 26.92810625316907 28.379722767044573 22.302714517245303 28-29 22.342648771124264 27.092310404108684 28.332175439266607 22.232865385500453 30-31 22.66136058181158 26.792733147714294 28.30781658626393 22.2380896842102 32-33 22.615316588252387 26.88241240398635 28.161028604927324 22.341242402833938 34-35 22.65959543756048 26.958053744075844 28.32381716111152 22.058533657252156 36-37 22.73136278377044 26.79501465091254 28.31544405618161 22.15817850913541 38-39 22.606150661334166 26.80936243541793 28.25944161517947 22.325045288068434 40-41 22.71621489615373 26.73856714189819 28.291627425834825 22.253590536113254 42-43 22.57662977708128 26.876476509699366 28.047273359185787 22.49962035403356 44-45 22.505584701713758 26.958943029545495 28.094200285900406 22.441271982840338 46-47 22.71027588203237 26.931926471327266 27.755857528451056 22.601940118189308 48-49 22.714139043018964 27.018822186339424 27.663006057805116 22.604032712836492 50-51 22.710593122909863 27.12227433263831 27.55105337732901 22.61607916712282 52-53 22.7604813039586 27.103872395688132 27.543763590471897 22.591882709881368 54-55 22.51527376763582 27.05598353888589 27.67839514784174 22.75034754563655 56-57 22.643557683350775 27.13510864723836 27.467700157262364 22.7536335121485 58-59 22.64330618305232 27.14850652056109 27.591357608516443 22.616829687870148 60-61 22.7068292365098 27.222032883179757 27.57141040323369 22.49972747707675 62-63 22.661399092976207 27.16709243469955 27.53571186827347 22.63579660405077 64-65 22.430885362142323 27.04612750722865 27.55738615023088 22.96560098039815 66-67 22.439507561084763 27.123597378974377 27.52054962811974 22.916345431821117 68-69 22.53371205811807 27.059292512559608 27.546759384979115 22.860236044343203 70-71 22.539027258679976 26.865751620018635 27.55537394313742 23.039847178163974 72-73 22.52029403936952 27.09995393005169 27.357638612036677 23.022113418542112 74-75 22.496185399547127 27.32947209821621 27.24325420296546 22.931088299271195 76-77 22.532213909022907 27.180079472594393 27.22426231608538 23.06344430229732 78-79 22.428344963646857 27.118497067294882 27.349490843065443 23.103667125992818 80-81 22.523655701045477 27.239345052712157 27.214720737884107 23.02227850835826 82-83 22.60201877258126 27.129025491387033 27.365608230380523 22.903347505651187 84-85 22.604890578296498 27.147161616759323 27.078048064210016 23.169899740734166 86-87 22.41421468869951 27.285818498318733 27.143803107056314 23.156163705925447 88-89 22.398188486904413 27.066341266399814 27.171506708641008 23.36396353805477 90-91 22.468386285733967 26.994131649251564 27.231401821169115 23.306080243845354 92-93 22.516892037354673 26.880083170434414 27.268989499576087 23.334035292634827 94-95 22.3077584178559 26.855985944762466 27.322693594913787 23.513562042467846 96-97 22.283282509189046 26.771967614479557 27.320367436647235 23.62438243968416 98-99 22.092441500053226 26.80665744109304 27.184363278877406 23.91653777997633 100-101 22.19347405463961 26.76977601893539 27.146245170662674 23.890504755762333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 256.0 1 201.0 2 165.0 3 204.5 4 196.5 5 201.5 6 340.5 7 648.0 8 1059.0 9 1285.5 10 1179.0 11 1054.5 12 1099.5 13 1262.5 14 1499.0 15 1766.5 16 2065.0 17 2408.5 18 2799.0 19 3170.0 20 3632.0 21 4320.5 22 5205.0 23 6454.0 24 8470.0 25 11321.5 26 14799.0 27 19073.5 28 24634.5 29 31670.0 30 39344.5 31 46969.5 32 55728.0 33 61945.0 34 65964.5 35 71200.5 36 78284.5 37 86217.0 38 91329.5 39 93966.0 40 96609.5 41 99807.5 42 103347.5 43 107570.0 44 111073.5 45 116444.5 46 121380.0 47 123504.0 48 124854.0 49 124166.0 50 120857.5 51 116973.0 52 115779.0 53 114834.0 54 111036.0 55 103196.5 56 93041.0 57 82272.0 58 72255.0 59 62331.0 60 51098.5 61 41266.5 62 33783.5 63 26450.5 64 19611.5 65 14687.5 66 10929.5 67 8401.0 68 6220.0 69 4439.5 70 3363.0 71 2520.5 72 1831.0 73 1395.5 74 1097.5 75 857.5 76 567.5 77 324.5 78 204.0 79 136.0 80 87.0 81 57.5 82 38.5 83 18.0 84 13.0 85 9.0 86 5.5 87 4.0 88 3.5 89 3.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019379966312045638 2 0.0031621592851641504 3 0.0037570209328682976 4 0.032247763007119554 5 0.0022542125597209786 6 6.261701554780496E-5 7 0.0 8 0.0 9 6.261701554780496E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.696276166085372E-5 24-25 6.418244093650008E-4 26-27 0.0019880902436428074 28-29 9.392552332170744E-5 30-31 0.006339972824215253 32-33 0.022667359628305395 34-35 0.013196536026699896 36-37 6.57478663251952E-4 38-39 6.887871710258546E-4 40-41 0.0021289785286253684 42-43 0.007983669482345133 44-45 0.007842781197362573 46-47 0.007686238658493059 48-49 0.009048158746657816 50-51 0.1919524611617961 52-53 0.04777678286297519 54-55 0.017360567560628926 56-57 0.009783908679344526 58-59 0.01945823758148039 60-61 0.05097025065591324 62-63 0.09154607673089085 64-65 0.09616408162754148 66-67 0.10043769293867917 68-69 0.09819913463284513 70-71 0.08932317267894377 72-73 0.06682800984339485 74-75 0.24754071671435995 76-77 0.298244845054195 78-79 0.006606095140293423 80-81 7.044414249128058E-4 82-83 0.0 84-85 3.130850777390248E-5 86-87 0.0 88-89 1.565425388695124E-4 90-91 0.006026887746476227 92-93 0.018080663239428682 94-95 0.0015810796425820752 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3194020.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.26472779366982 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53499557142237 43.82755688087228 2 13.130541383279674 15.038337561940548 3 4.294989762660459 7.378542589060494 4 1.9172018856328819 4.391521764251101 5 1.0810994318919398 3.0954432342591516 6 0.6579869029037346 2.260766453194933 7 0.47426345114313834 1.9010997202538775 8 0.32010981324784016 1.4664801055776042 9 0.24309228693772383 1.252855227620649 >10 1.2464394749485754 12.707705512137077 >50 0.07035614306978415 2.744669083245537 >100 0.026898407438188065 2.7349445771520076 >500 0.0015875426296555492 0.6420140775499709 >1k 3.8319994471451915E-4 0.3925753069855639 >5k 5.47428492449313E-5 0.16548790589928863 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5279 0.1652776125384312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4406 0.13794528525181432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.130850777390248E-5 7 0.0 0.0 0.0 0.0 3.130850777390248E-5 8 0.0 0.0 0.0 0.0 3.130850777390248E-5 9 0.0 0.0 0.0 3.130850777390248E-5 3.130850777390248E-5 10-11 0.0 0.0 0.0 3.130850777390248E-5 3.130850777390248E-5 12-13 0.0 0.0 0.0 4.696276166085372E-5 1.4088828498256116E-4 14-15 0.0 0.0 0.0 6.261701554780496E-5 1.565425388695124E-4 16-17 0.0 0.0 0.0 6.261701554780496E-5 1.8785104664341488E-4 18-19 0.0 0.0 0.0 6.261701554780496E-5 2.1915955441731736E-4 20-21 0.0 0.0 0.0 6.261701554780496E-5 2.5046806219121984E-4 22-23 0.0 0.0 0.0 1.4088828498256116E-4 2.817765699651223E-4 24-25 0.0 0.0 0.0 2.1915955441731736E-4 2.817765699651223E-4 26-27 0.0 0.0 0.0 6.261701554780496E-4 2.817765699651223E-4 28-29 0.0 0.0 0.0 0.001628042404242929 2.817765699651223E-4 30-31 0.0 0.0 0.0 0.006183430285345739 2.817765699651223E-4 32-33 0.0 0.0 0.0 0.018456365332715514 2.817765699651223E-4 34-35 0.0 0.0 0.0 0.036396140287161635 3.443935855129273E-4 36-37 0.0 0.0 0.0 0.06496515363084765 3.443935855129273E-4 38-39 0.0 0.0 0.0 0.10657416046236404 3.443935855129273E-4 40-41 0.0 0.0 0.0 0.15433528907145228 3.443935855129273E-4 42-43 0.0 0.0 0.0 0.20272258783601854 3.443935855129273E-4 44-45 0.0 0.0 0.0 0.26244356641473754 4.383191088346347E-4 46-47 0.0 0.0 0.0 0.33235546427386176 5.16590378269391E-4 48-49 0.0 0.0 0.0 0.4046468087238026 5.322446321563422E-4 50-51 0.0 0.0 0.0 0.4800533496972467 5.948616477041472E-4 52-53 0.0 0.0 0.0 0.5566809224738731 7.200956787997571E-4 54-55 0.0 0.0 0.0 0.6444543240180086 7.514041865736595E-4 56-57 0.0 0.0 0.0 0.7455651498738267 7.670584404606108E-4 58-59 0.0 0.0 0.0 0.8584636289065191 7.827126943475621E-4 60-61 0.0 0.0 0.0 0.9803476496703214 7.827126943475621E-4 62-63 0.0 0.0 0.0 1.1032335426828888 7.827126943475621E-4 64-65 0.0 0.0 0.0 1.2337587115922881 7.827126943475621E-4 66-67 0.0 0.0 0.0 1.3859180593734541 7.827126943475621E-4 68-69 0.0 0.0 0.0 1.5578330755599528 7.827126943475621E-4 70-71 0.0 0.0 0.0 1.7426785054570728 8.140212021214645E-4 72-73 0.0 0.0 0.0 1.9338639081784084 9.07946725443172E-4 74-75 0.0 0.0 0.0 2.135005416371845 9.705637409909769E-4 76-77 0.0 3.130850777390248E-5 0.0 2.3605362521211513 0.0010018722487648794 78-79 0.0 3.130850777390248E-5 0.0 2.62009943582069 0.0010175265026518307 80-81 0.0 3.130850777390248E-5 0.0 2.901594229215847 0.0010331807565387818 82-83 0.0 3.130850777390248E-5 0.0 3.197553553202547 0.0010331807565387818 84-85 0.0 3.130850777390248E-5 0.0 3.5099811522783204 0.0010331807565387818 86-87 0.0 3.130850777390248E-5 0.0 3.839925861453591 0.0010957977720865869 88-89 0.0 3.130850777390248E-5 0.0 4.203777684547999 0.0011584147876343917 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3635 0.0 54.34183 1 GTATCAA 6830 0.0 43.034355 1 ATCAACG 8550 0.0 34.035404 3 CAACGCA 8580 0.0 33.571804 5 TCAACGC 8675 0.0 33.488686 4 TATCAAC 8805 0.0 33.319805 2 AACGCAG 8950 0.0 32.448517 6 ACGCAGA 10455 0.0 27.459831 7 CGCAGAG 10800 0.0 26.538702 8 GTACATG 9070 0.0 24.972178 1 TACATGG 9350 0.0 24.06634 2 GCAGAGT 12245 0.0 22.748138 9 ACATGGG 9695 0.0 22.670948 3 GTACTAG 710 0.0 20.732357 1 CATGGGG 7085 0.0 20.569069 4 AGAGTAC 11305 0.0 19.917377 10-11 GTGGTAT 1770 0.0 19.85198 1 CAGAGTA 11900 0.0 19.539598 10-11 GAGTACT 7685 0.0 19.38886 12-13 GAGTACA 7125 0.0 18.127144 1 >>END_MODULE