FastQCFastQC Report
Sat 18 Jun 2016
SRR1930967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224644
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41150.1849734159712745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.063.491121
GTATCAA54450.045.358161
GTGGTAT10850.039.4058761
TATCAAC67100.036.8676152
ATCAACG68200.036.858363
TCAACGC69100.036.435764
CAACGCA72200.034.1835255
TGGTATC13150.031.736192
AACGCAG77250.031.3502456
ACGCAGA88500.027.271587
GTACATG65850.026.8880121
TACATGG66650.026.0268382
CGCAGAG93250.025.7148388
ACATGGG71300.024.1854953
GCAGAGT98700.022.7196189
CATGGGG52100.021.8955734
GAGTACT57300.021.44629112-13
CAGAGTA93600.020.97301110-11
GTACTTT62450.019.71421814-15
AGAGTAC88150.018.85517910-11
Produced by FastQC (version 0.11.5)