##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930967_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2224644 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.546359327604776 30.0 16.0 31.0 2.0 33.0 2 22.377163716981233 30.0 10.0 31.0 2.0 33.0 3 22.860988544684 30.0 16.0 31.0 2.0 34.0 4 24.84171984371432 33.0 10.0 35.0 2.0 37.0 5 24.844917209225386 33.0 10.0 35.0 2.0 37.0 6 25.62540568288679 33.0 17.0 35.0 2.0 37.0 7 25.738006620385104 33.0 17.0 35.0 2.0 37.0 8 26.562319184552674 33.0 17.0 35.0 2.0 37.0 9 27.76890190070861 34.0 17.0 38.0 2.0 39.0 10-11 28.0479917236196 34.5 24.0 37.0 2.0 39.0 12-13 27.998286242652757 35.0 24.0 37.0 2.0 39.0 14-15 28.271344763476762 35.0 21.0 38.0 2.0 40.0 16-17 27.39543989959742 34.0 17.0 38.0 2.0 40.0 18-19 26.79685401349609 34.0 13.5 38.0 2.0 40.0 20-21 26.26243884414765 33.0 10.0 38.0 2.0 40.0 22-23 26.227948381853455 33.0 11.0 38.0 2.0 40.0 24-25 25.913304555695206 33.0 10.0 38.0 2.0 40.0 26-27 25.566229473120195 32.5 10.0 38.0 2.0 40.0 28-29 25.492083002943396 32.0 9.5 38.0 2.0 40.0 30-31 25.40743148117182 32.0 9.0 38.0 2.0 40.0 32-33 25.092142832740876 32.0 8.0 38.0 2.0 40.0 34-35 22.008335490981928 27.5 2.0 37.0 2.0 39.0 36-37 20.317381792322728 21.0 2.0 36.0 2.0 38.5 38-39 19.31041303687242 19.5 2.0 35.0 2.0 38.0 40-41 20.685297512770582 24.5 2.0 35.0 2.0 38.0 42-43 20.57732113542661 24.5 2.0 35.0 2.0 39.0 44-45 18.517813636698726 19.5 2.0 34.0 2.0 38.0 46-47 19.35916398309123 19.0 2.0 33.5 2.0 38.0 48-49 18.747182245788537 20.5 2.0 34.0 2.0 38.0 50-51 16.65492883355719 11.0 2.0 32.5 2.0 37.5 52-53 19.632356008422022 23.0 2.0 33.5 2.0 38.0 54-55 21.374671408099452 27.5 2.0 35.5 2.0 38.5 56-57 21.60358915853503 28.0 2.0 36.0 2.0 39.0 58-59 21.227286702951123 28.0 2.0 36.0 2.0 39.0 60-61 20.896694707108193 28.0 2.0 35.0 2.0 39.0 62-63 20.407750633359765 26.5 2.0 35.0 2.0 38.5 64-65 19.900667028072803 26.0 2.0 34.0 2.0 38.0 66-67 19.47640836016909 26.0 2.0 34.0 2.0 37.5 68-69 19.006555206136355 24.5 2.0 34.0 2.0 37.0 70-71 18.41103632761017 22.5 2.0 33.5 2.0 36.0 72-73 17.93535639859681 21.5 2.0 33.0 2.0 36.0 74-75 17.40864673179169 19.5 2.0 32.5 2.0 35.0 76-77 16.843099615039527 17.0 2.0 32.0 2.0 35.0 78-79 16.352911072513173 12.0 2.0 31.5 2.0 35.0 80-81 15.847866220393016 7.5 2.0 31.0 2.0 34.0 82-83 15.379254838077463 4.5 2.0 31.0 2.0 34.0 84-85 14.857376281328609 2.0 2.0 31.0 2.0 34.0 86-87 14.306204498337712 2.0 2.0 30.0 2.0 34.0 88-89 13.753843761069188 2.0 2.0 29.5 2.0 34.0 90-91 13.170943755495262 2.0 2.0 29.0 2.0 34.0 92-93 12.537088630810143 2.0 2.0 29.0 2.0 34.0 94-95 11.864067239522369 2.0 2.0 28.0 2.0 34.0 96-97 11.115533991056548 2.0 2.0 26.5 2.0 33.5 98-99 10.291384149553817 2.0 2.0 25.0 2.0 33.0 100-101 9.184685055226813 2.0 2.0 20.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.875115048792956 1101 2 8.037287172082028 1101 3 7.6811192043788665 1101 4 9.579296681316976 1101 5 9.315652603761336 1101 6 8.82463111287677 1101 7 8.615576581190176 1101 8 7.561194009539701 1101 9 7.876318196334921 1101 10-11 7.1910607458100095 1101 12-13 7.003954978761342 1101 14-15 7.566310735643146 1101 16-17 8.357475033716202 1101 18-19 8.81849126273892 1101 20-21 9.11002531326531 1101 22-23 9.01375420054228 1101 24-25 8.933653168056019 1101 26-27 8.989397410515174 1101 28-29 8.93286911168444 1101 30-31 8.775231876636656 1101 32-33 8.732870953218235 1101 34-35 11.340935516287615 1101 36-37 5.320901889339421 1101 38-39 8.672938579775337 1101 40-41 10.43723503750924 1101 42-43 10.843974000633747 1101 44-45 4.765526576044341 1101 46-47 6.440009310508049 1101 48-49 9.776762611754066 1101 50-51 -3.8158561819773915 1101 52-53 6.172283316026487 1101 54-55 8.618453483721833 1101 56-57 9.18300032391075 1101 58-59 9.47815654204398 1101 60-61 9.223957479988524 1101 62-63 9.420387199086793 1101 64-65 9.300567041218127 1101 66-67 9.449858607914958 1101 68-69 9.332915137173433 1101 70-71 9.365459302568745 1101 72-73 9.120266765296865 1101 74-75 9.250286593937055 1101 76-77 9.059323089420381 1101 78-79 9.018891662651939 1101 80-81 8.88657893245085 1101 82-83 8.878667208198081 1101 84-85 8.837925678382113 1101 86-87 8.68051736581795 1101 88-89 8.412434470687446 1101 90-91 8.244899121717125 1101 92-93 8.291343791590764 1101 94-95 8.024884814095408 1101 96-97 7.854545070524843 1101 98-99 7.553534831162983 1101 100-101 6.897054838785554 1102 1 6.690209999886335 1102 2 7.899051941831399 1102 3 7.635317429715204 1102 4 9.361864160925606 1102 5 9.45038609673788 1102 6 8.65652819866131 1102 7 8.360100668817914 1102 8 7.456110870194831 1102 9 7.452920204642382 1102 10-11 7.008139315196143 1102 12-13 6.813003730644738 1102 14-15 7.175348520647429 1102 16-17 7.932920775309892 1102 18-19 8.400819496503882 1102 20-21 8.787061672027875 1102 22-23 8.50726458176085 1102 24-25 8.610082516446397 1102 26-27 8.610068572568238 1102 28-29 8.52049356340892 1102 30-31 8.397489677855855 1102 32-33 8.48085836667158 1102 34-35 10.67767846632701 1102 36-37 0.8775846875979312 1102 38-39 3.3353336987294817 1102 40-41 8.869615012681393 1102 42-43 10.03390538373603 1102 44-45 1.185977013688511 1102 46-47 1.7623986960288853 1102 48-49 7.934669156791173 1102 50-51 -4.864729595178558 1102 52-53 5.2385377728037525 1102 54-55 7.931031435696394 1102 56-57 8.414474372825953 1102 58-59 8.731856768881375 1102 60-61 8.701902739386234 1102 62-63 8.675520693012214 1102 64-65 8.585070238857163 1102 66-67 8.52353313466493 1102 68-69 8.583658686102961 1102 70-71 8.530948413516153 1102 72-73 8.255628892192597 1102 74-75 8.022127790607868 1102 76-77 8.127231884401237 1102 78-79 8.005592360098007 1102 80-81 7.87798453052258 1102 82-83 7.831477785743864 1102 84-85 7.699810153168052 1102 86-87 7.625587169729792 1102 88-89 7.405947315348081 1102 90-91 7.34900454827854 1102 92-93 7.128428521406827 1102 94-95 6.9918764239766915 1102 96-97 6.9306651565737845 1102 98-99 6.344258676205543 1102 100-101 5.976972001440428 1103 1 6.682940368701942 1103 2 7.779542833470671 1103 3 7.475075655572482 1103 4 9.383873804136904 1103 5 9.14802296066322 1103 6 8.603320143233443 1103 7 8.26727193086289 1103 8 7.415980814772741 1103 9 7.688802679179155 1103 10-11 7.127036778558871 1103 12-13 6.81338860622693 1103 14-15 7.305451464927561 1103 16-17 7.922030874300614 1103 18-19 8.479374229064138 1103 20-21 8.703146959734113 1103 22-23 8.86368313196887 1103 24-25 8.814201281461962 1103 26-27 8.76726558333175 1103 28-29 8.804966399504679 1103 30-31 8.412100675250993 1103 32-33 8.289592729519992 1103 34-35 10.816659930140752 1103 36-37 -3.093123118473926 1103 38-39 -1.8122484694969287 1103 40-41 7.034270749604296 1103 42-43 9.51299498493988 1103 44-45 0.9576389136801247 1103 46-47 0.5174149077834578 1103 48-49 8.145088226671568 1103 50-51 -5.428277884234937 1103 52-53 4.57295517236026 1103 54-55 7.800468899377147 1103 56-57 8.485520236985472 1103 58-59 8.656834308771106 1103 60-61 8.583145041874591 1103 62-63 8.646897785073012 1103 64-65 8.585954097500498 1103 66-67 8.470225878910949 1103 68-69 8.288679559730777 1103 70-71 8.35006646660224 1103 72-73 8.21409937133198 1103 74-75 8.302560788286325 1103 76-77 8.247401605498343 1103 78-79 8.148891929181907 1103 80-81 7.878153116869148 1103 82-83 8.003625676987056 1103 84-85 7.744553475486356 1103 86-87 7.492986057674257 1103 88-89 7.388455262215738 1103 90-91 7.428822052203188 1103 92-93 7.3775326466948545 1103 94-95 6.954503559403747 1103 96-97 6.840019187178804 1103 98-99 6.742171873571186 1103 100-101 6.230991150536532 1104 1 6.431630336906306 1104 2 7.592383935414183 1104 3 7.046898273976176 1104 4 8.922486651463466 1104 5 8.798217104370345 1104 6 8.124550206567147 1104 7 7.799308325563807 1104 8 6.6964395906263725 1104 9 6.884096509795121 1104 10-11 6.378133420412919 1104 12-13 6.148836521194347 1104 14-15 6.621056977220828 1104 16-17 7.288921251612667 1104 18-19 7.832558014147377 1104 20-21 8.27962274882973 1104 22-23 8.055807147119737 1104 24-25 8.100997094654716 1104 26-27 8.103355546241602 1104 28-29 7.873702376226042 1104 30-31 7.564817578166977 1104 32-33 7.465739597606731 1104 34-35 9.992686598696267 1104 36-37 -3.4471884562202995 1104 38-39 -2.263859304196817 1104 40-41 6.094273389701144 1104 42-43 8.291730453675125 1104 44-45 0.15494170416948094 1104 46-47 -0.6981209174006331 1104 48-49 6.811726095533878 1104 50-51 -5.870125547785001 1104 52-53 3.6139297079464754 1104 54-55 6.679661662936088 1104 56-57 7.158312429167349 1104 58-59 7.429108694966477 1104 60-61 7.3256446447868555 1104 62-63 7.586839964090178 1104 64-65 7.6549515797496 1104 66-67 7.281668588093908 1104 68-69 7.161059496139504 1104 70-71 7.281021673674555 1104 72-73 7.060533559226744 1104 74-75 6.966855660631559 1104 76-77 6.916318793745123 1104 78-79 6.7363563164934135 1104 80-81 6.537632674363158 1104 82-83 6.458569007916402 1104 84-85 6.2916607807559615 1104 86-87 6.1931376843745785 1104 88-89 6.236149845939005 1104 90-91 5.868971539638043 1104 92-93 5.751512381626265 1104 94-95 5.390750407743418 1104 96-97 4.983371795365535 1104 98-99 4.553386078436979 1104 100-101 4.306011577302105 1105 1 5.829839832004851 1105 2 6.986987369073859 1105 3 6.559146092792595 1105 4 8.586389358097186 1105 5 8.48411028504021 1105 6 7.6641023706972895 1105 7 7.511550295475068 1105 8 6.560175427291117 1105 9 6.645614801012812 1105 10-11 6.014571817700155 1105 12-13 5.638932140223446 1105 14-15 6.044245349315492 1105 16-17 6.695256056333225 1105 18-19 7.379200742584121 1105 20-21 7.785327633511166 1105 22-23 7.584787591340557 1105 24-25 7.6814574175847525 1105 26-27 7.720638955293595 1105 28-29 7.333389766338854 1105 30-31 7.113872715725474 1105 32-33 6.661338789753135 1105 34-35 9.151525157638787 1105 36-37 -3.894121093040635 1105 38-39 -2.8016160129967425 1105 40-41 5.390779056444622 1105 42-43 7.481704390812784 1105 44-45 -3.6361367247159375 1105 46-47 -4.736736375845574 1105 48-49 4.711596362589248 1105 50-51 -10.149991255513097 1105 52-53 0.07302674621593752 1105 54-55 4.724753374473718 1105 56-57 5.6753640136921355 1105 58-59 5.976558995128062 1105 60-61 5.995719179569871 1105 62-63 6.217077552960067 1105 64-65 6.110924826998321 1105 66-67 5.970247244241005 1105 68-69 5.880635940272949 1105 70-71 6.069977672290548 1105 72-73 6.009276361726126 1105 74-75 5.68010753562848 1105 76-77 5.706431448591363 1105 78-79 5.398004199718262 1105 80-81 5.434149510401435 1105 82-83 5.388130044060517 1105 84-85 5.284495960017857 1105 86-87 5.0848516355637035 1105 88-89 4.971460637732777 1105 90-91 4.81191203442723 1105 92-93 4.51107023258348 1105 94-95 4.31454569183461 1105 96-97 4.168301756487931 1105 98-99 4.034914284605954 1105 100-101 3.7266133944089557 1106 1 5.9960148804106055 1106 2 7.229037793539227 1106 3 6.852653020125196 1106 4 8.342823869595996 1106 5 8.339298722096231 1106 6 7.273355894505638 1106 7 7.084757254906336 1106 8 6.663913410051318 1106 9 6.412366766283014 1106 10-11 5.938859856169071 1106 12-13 5.956401720376974 1106 14-15 6.322792716159288 1106 16-17 6.716874801818282 1106 18-19 7.288426635418556 1106 20-21 7.7174522663869425 1106 22-23 7.35814676169651 1106 24-25 7.4174948403709635 1106 26-27 7.5254977795750335 1106 28-29 7.449410558990394 1106 30-31 7.153269106103021 1106 32-33 7.058810521273546 1106 34-35 9.74793294201271 1106 36-37 -0.14014209533015887 1106 38-39 0.8230291489365733 1106 40-41 6.8804050691507435 1106 42-43 8.302771066689775 1106 44-45 -3.3963776262555037 1106 46-47 -4.593794543169679 1106 48-49 4.791634771017659 1106 50-51 -9.939572281969411 1106 52-53 0.4627163548389994 1106 54-55 5.190973649697465 1106 56-57 6.337170601686136 1106 58-59 6.759980445149335 1106 60-61 6.5300606310483005 1106 62-63 6.4797762021353655 1106 64-65 6.543165362186919 1106 66-67 6.446720621247771 1106 68-69 6.080349715688115 1106 70-71 6.060691740511558 1106 72-73 6.070320881273666 1106 74-75 5.701616307088017 1106 76-77 5.506308019931165 1106 78-79 5.482650519815532 1106 80-81 5.127550690835131 1106 82-83 5.061303564514656 1106 84-85 4.943819712551555 1106 86-87 4.860604201567023 1106 88-89 4.661946475483974 1106 90-91 4.516881193586453 1106 92-93 4.341598884597483 1106 94-95 4.245541630356378 1106 96-97 4.012832937217183 1106 98-99 3.8791726782016163 1106 100-101 3.6739128103781464 1107 1 5.824185014708494 1107 2 7.332622954407935 1107 3 6.749896139321535 1107 4 8.151561928769475 1107 5 8.294490374789369 1107 6 7.071421890105025 1107 7 7.011399334910934 1107 8 6.50290657273025 1107 9 6.707134332828268 1107 10-11 6.052251988099481 1107 12-13 5.702220805773415 1107 14-15 5.968285302166162 1107 16-17 6.838560541723137 1107 18-19 7.2201296670418 1107 20-21 7.6651645754223665 1107 22-23 7.453427085105673 1107 24-25 7.425627858265528 1107 26-27 7.493398845278499 1107 28-29 7.335033271932673 1107 30-31 7.192506109029448 1107 32-33 6.847513490416947 1107 34-35 9.32187639944452 1107 36-37 -0.3369969951851033 1107 38-39 0.44797608328714844 1107 40-41 6.951996667131802 1107 42-43 7.840883516895872 1107 44-45 -3.391412589637252 1107 46-47 -4.662274560596488 1107 48-49 4.8987698845198935 1107 50-51 -10.015408878607996 1107 52-53 0.14560413935446803 1107 54-55 4.896009862676049 1107 56-57 6.084149194741414 1107 58-59 6.475012166333887 1107 60-61 6.368978480188186 1107 62-63 6.406270947918397 1107 64-65 6.3656997303215235 1107 66-67 6.213515540454576 1107 68-69 5.91900835791672 1107 70-71 5.9239295303462605 1107 72-73 5.848905577765169 1107 74-75 5.929519720007164 1107 76-77 5.86212193807582 1107 78-79 5.876940031138737 1107 80-81 5.620152570971774 1107 82-83 5.3019832607613075 1107 84-85 5.2698946613434146 1107 86-87 5.146612600884135 1107 88-89 5.043964101553941 1107 90-91 4.909593342200669 1107 92-93 4.873591687595161 1107 94-95 4.459520566206468 1107 96-97 4.392948136536322 1107 98-99 3.9293732047209318 1107 100-101 3.4443036977776504 1108 1 6.046743749586071 1108 2 6.4226018965607885 1108 3 6.743451818746781 1108 4 7.987741856374527 1108 5 8.187495522263113 1108 6 7.4799682104870975 1108 7 7.577907723641545 1108 8 6.589492861731994 1108 9 4.871386916887616 1108 10-11 5.843328897413105 1108 12-13 5.3369151573155875 1108 14-15 5.977153339657388 1108 16-17 6.148392698294419 1108 18-19 6.725581825815208 1108 20-21 7.4339382310921565 1108 22-23 7.522707402212085 1108 24-25 7.218608403842694 1108 26-27 7.498710962486754 1108 28-29 7.305767681555963 1108 30-31 7.010684674067914 1108 32-33 7.217444639514763 1108 34-35 9.479730425897579 1108 36-37 -3.796501585245249 1108 38-39 -2.635645763197015 1108 40-41 5.444430465664631 1108 42-43 7.974360676701533 1108 44-45 -3.342452234819408 1108 46-47 -4.644845063094527 1108 48-49 4.887921480374384 1108 50-51 -10.06303333323728 1108 52-53 0.46849629014516836 1108 54-55 5.132636925450452 1108 56-57 6.448799194053247 1108 58-59 7.004917061939263 1108 60-61 6.982590898812685 1108 62-63 6.9447607011424495 1108 64-65 6.796577519109938 1108 66-67 6.968879270033284 1108 68-69 6.838192676014085 1108 70-71 6.645471501525908 1108 72-73 6.34317901322218 1108 74-75 6.31641791976768 1108 76-77 6.428056225205761 1108 78-79 6.197447346332044 1108 80-81 6.183392520322835 1108 82-83 6.002764672049963 1108 84-85 5.882621411690938 1108 86-87 5.714314095578382 1108 88-89 5.724722837807848 1108 90-91 5.434706889415665 1108 92-93 5.476119489458712 1108 94-95 5.192812093519734 1108 96-97 4.897125641800784 1108 98-99 4.638037201307634 1108 100-101 4.281876471219988 1109 1 4.888088056721198 1109 2 6.325661175632945 1109 3 6.032594529760775 1109 4 7.5770932984975445 1109 5 7.388877600794409 1109 6 4.793881296175897 1109 7 4.711803066834321 1109 8 3.1810841005127877 1109 9 2.9013451425254537 1109 10-11 2.601944947547967 1109 12-13 3.212151624265445 1109 14-15 3.875789833762685 1109 16-17 3.0964517522437127 1109 18-19 4.168275304849939 1109 20-21 4.619547023125058 1109 22-23 5.797077656164749 1109 24-25 5.844371095496115 1109 26-27 4.7438687982399195 1109 28-29 4.400206372860453 1109 30-31 4.237303905470302 1109 32-33 3.83763694720707 1109 34-35 6.487007170846034 1109 36-37 -4.808778696018363 1109 38-39 -3.8261226613697215 1109 40-41 2.8532132298357666 1109 42-43 3.949702687583411 1109 44-45 -4.866303562973977 1109 46-47 -6.497027101666518 1109 48-49 1.7238001384451103 1109 50-51 -10.344673297048779 1109 52-53 -1.4992381506988721 1109 54-55 2.0610247256201397 1109 56-57 3.5091748608038706 1109 58-59 4.3925079377094285 1109 60-61 4.431029583785591 1109 62-63 4.417088176122153 1109 64-65 4.051880481726883 1109 66-67 4.399674477870935 1109 68-69 4.575026681667481 1109 70-71 4.498943423779725 1109 72-73 4.259313533984841 1109 74-75 4.4317273476319805 1109 76-77 4.217749168166659 1109 78-79 4.207117780523703 1109 80-81 4.057826195608426 1109 82-83 3.918602663785144 1109 84-85 3.7748328463849035 1109 86-87 3.945346350760781 1109 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-10.176430677765367 2313 94-95 -9.607146100460199 2313 96-97 -8.847188739470118 2313 98-99 -7.993885875615444 2313 100-101 -6.91411015085359 2314 1 -21.21161745442731 2314 2 -20.015525193740213 2314 3 -20.47602978660439 2314 4 -22.40481667205357 2314 5 -22.39151785566331 2314 6 -23.198125434997852 2314 7 -23.306707660973842 2314 8 -24.15799877706399 2314 9 -25.34265990585486 2314 10-11 -25.638694513958132 2314 12-13 -25.59222363867103 2314 14-15 -25.8267764262289 2314 16-17 -24.954032050869454 2314 18-19 -24.359911485221577 2314 20-21 -23.845535013055 2314 22-23 -23.789165507486913 2314 24-25 -23.47725280017069 2314 26-27 -23.117300743198868 2314 28-29 -23.05961693382865 2314 30-31 -22.967576195497305 2314 32-33 -22.66236305279293 2314 34-35 -19.706724423600747 2314 36-37 -18.017655431399096 2314 38-39 -17.051406632762234 2314 40-41 -18.40067822998754 2314 42-43 -18.28483664771318 2314 44-45 -16.262811766591984 2314 46-47 -17.035731350719946 2314 48-49 -16.470983201899863 2314 50-51 -14.399153734575073 2314 52-53 -17.311185984102323 2314 54-55 -18.985883141439178 2314 56-57 -19.185398130361467 2314 58-59 -18.81531705177311 2314 60-61 -18.47999271322745 2314 62-63 -18.00339983397461 2314 64-65 -17.493765290254608 2314 66-67 -17.097801331973404 2314 68-69 -16.625436086527724 2314 70-71 -16.034712444962384 2314 72-73 -15.566938043634007 2314 74-75 -15.056282749128641 2314 76-77 -14.511952135998254 2314 78-79 -14.034994125239862 2314 80-81 -13.518530556600242 2314 82-83 -13.060362418251039 2314 84-85 -12.53648393947963 2314 86-87 -11.996597275659076 2314 88-89 -11.4527437173761 2314 90-91 -10.88176468249069 2314 92-93 -10.264474156026237 2314 94-95 -9.607146100460199 2314 96-97 -8.87001482642664 2314 98-99 -8.047581527789356 2314 100-101 -6.9760666725927205 2315 1 -21.21161745442731 2315 2 -20.015525193740213 2315 3 -20.47602978660439 2315 4 -17.502154079299316 2315 5 -22.39151785566331 2315 6 -23.198125434997852 2315 7 -23.306707660973842 2315 8 -24.15799877706399 2315 9 -20.854226907164332 2315 10-11 -19.57082022325538 2315 12-13 -16.118631320905862 2315 14-15 -15.974964990174776 2315 16-17 -20.952067843099922 2315 18-19 -24.359911485221577 2315 20-21 -23.845535013055 2315 22-23 -23.789165507486913 2315 24-25 -23.47725280017069 2315 26-27 -23.117300743198868 2315 28-29 -23.05961693382865 2315 30-31 -22.967576195497305 2315 32-33 -22.66236305279293 2315 34-35 -19.706724423600747 2315 36-37 -18.017655431399096 2315 38-39 -17.051406632762234 2315 40-41 -18.40067822998754 2315 42-43 -18.28483664771318 2315 44-45 -16.262811766591984 2315 46-47 -17.035731350719946 2315 48-49 -16.470983201899863 2315 50-51 -14.399153734575073 2315 52-53 -17.311185984102323 2315 54-55 -18.985883141439178 2315 56-57 -19.185398130361467 2315 58-59 -18.81531705177311 2315 60-61 -18.47999271322745 2315 62-63 -18.00339983397461 2315 64-65 -17.493765290254608 2315 66-67 -17.097801331973404 2315 68-69 -16.625436086527724 2315 70-71 -16.034712444962384 2315 72-73 -15.566938043634007 2315 74-75 -15.056282749128641 2315 76-77 -14.511952135998254 2315 78-79 -14.034994125239862 2315 80-81 -13.518530556600242 2315 82-83 -13.060362418251039 2315 84-85 -12.53648393947963 2315 86-87 -11.996597275659076 2315 88-89 -11.4527437173761 2315 90-91 -10.88176468249069 2315 92-93 -10.264474156026237 2315 94-95 -9.607146100460199 2315 96-97 -8.87001482642664 2315 98-99 -8.047581527789356 2315 100-101 -6.9760666725927205 2316 1 -21.21161745442731 2316 2 -20.015525193740213 2316 3 -20.47602978660439 2316 4 -22.40481667205357 2316 5 -22.39151785566331 2316 6 -23.198125434997852 2316 7 -23.306707660973842 2316 8 -24.15799877706399 2316 9 -25.34265990585486 2316 10-11 -25.638694513958132 2316 12-13 -25.59222363867103 2316 14-15 -25.8267764262289 2316 16-17 -24.954032050869454 2316 18-19 -24.359911485221577 2316 20-21 -23.845535013055 2316 22-23 -23.789165507486913 2316 24-25 -23.47725280017069 2316 26-27 -23.117300743198868 2316 28-29 -23.05961693382865 2316 30-31 -22.967576195497305 2316 32-33 -22.66236305279293 2316 34-35 -19.706724423600747 2316 36-37 -18.017655431399096 2316 38-39 -17.051406632762234 2316 40-41 -18.40067822998754 2316 42-43 -18.28483664771318 2316 44-45 -16.262811766591984 2316 46-47 -17.035731350719946 2316 48-49 -16.470983201899863 2316 50-51 -14.399153734575073 2316 52-53 -17.311185984102323 2316 54-55 -18.985883141439178 2316 56-57 -19.185398130361467 2316 58-59 -18.81531705177311 2316 60-61 -18.47999271322745 2316 62-63 -18.00339983397461 2316 64-65 -17.493765290254608 2316 66-67 -17.097801331973404 2316 68-69 -16.625436086527724 2316 70-71 -16.034712444962384 2316 72-73 -15.566938043634007 2316 74-75 -15.056282749128641 2316 76-77 -14.511952135998254 2316 78-79 -14.034994125239862 2316 80-81 -13.518530556600242 2316 82-83 -13.060362418251039 2316 84-85 -12.53648393947963 2316 86-87 -11.996597275659076 2316 88-89 -11.4527437173761 2316 90-91 -10.88176468249069 2316 92-93 -10.264474156026237 2316 94-95 -9.607146100460199 2316 96-97 -8.87001482642664 2316 98-99 -8.047581527789356 2316 100-101 -6.9760666725927205 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 346652.0 3 19345.0 4 42990.0 5 24404.0 6 20667.0 7 26819.0 8 35083.0 9 42783.0 10 46742.0 11 44549.0 12 40941.0 13 38153.0 14 35496.0 15 37843.0 16 40873.0 17 42579.0 18 43788.0 19 45187.0 20 48141.0 21 51398.0 22 54699.0 23 58912.0 24 62247.0 25 66912.0 26 71715.0 27 76694.0 28 81674.0 29 87816.0 30 91723.0 31 91867.0 32 90303.0 33 88255.0 34 82714.0 35 70197.0 36 49756.0 37 21702.0 38 3004.0 39 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25862688618996 18.417104040252692 13.342243215688656 23.982025857868692 2 17.14299866263807 20.209423249146145 38.248380391507155 24.399197696708633 3 19.652635555966345 24.59522523881103 29.297612078895067 26.45452712632756 4 13.916602431463392 16.64521009091323 35.69934119760555 33.738846280017825 5 14.729996278270333 36.229456426138626 33.446672560843375 15.593874734747667 6 32.03462232736487 34.59792814884696 17.654247625881535 15.713201897906634 7 27.964393410674077 31.876114351931108 21.435904188778128 18.72358804861669 8 26.641708122655356 33.175970477899206 20.275087669221985 19.907233730223457 9 27.028675824130012 15.532406099252277 20.163311287359495 37.27560678925821 10-11 25.895515006952287 25.21334589354167 27.24357681700506 21.647562282500978 12-13 26.315289471723418 23.689739959764548 27.247527196185082 22.747443372326952 14-15 23.709896253740578 24.94722784750926 25.41639172966894 25.926484169081228 16-17 23.024144719895464 27.63850724047788 26.22754296884795 23.1098050707787 18-19 22.948141691956813 26.162127550450503 28.514019578691563 22.37571117890112 20-21 23.892929534116547 25.26675769648697 28.449059011517353 22.391253757879127 22-23 23.764168707015358 25.236101166992842 28.123907415463705 22.875822710528094 24-25 23.31214573858663 25.632787262122918 28.12582353653172 22.929243462758734 26-27 22.999797578253002 25.841479563902176 28.249422737473417 22.909300120371405 28-29 23.02601552978362 25.9364848951889 28.345898783393825 22.691600791633658 30-31 23.40845792565229 25.53245536308573 28.197436185436636 22.861650525825347 32-33 23.24755739873349 25.750800355287456 27.936763944713185 23.06487830126587 34-35 23.641384997934093 25.845324882370726 27.791490454840584 22.721799664854597 36-37 23.938498229555126 25.920566589445137 27.45216620522093 22.68876897577881 38-39 23.670807036136647 26.006995652946514 27.566926795758867 22.755270515157974 40-41 23.82670946944449 25.798538298083585 27.59630419876214 22.778448033709786 42-43 23.89168564770894 25.89264635804439 27.174139951122907 23.041528043123762 44-45 23.918789124468077 26.244188401142992 26.910445785537064 22.926576688851867 46-47 23.49089462967349 26.201259949164275 27.022472227631432 23.285373193530802 48-49 23.868617846089222 26.39987763751369 26.576972224742907 23.15453229165418 50-51 24.120735959780067 26.555341198521987 26.39791239172152 22.926010449976427 52-53 23.363080210533973 26.556106373782622 26.779031228666874 23.301782187016535 54-55 23.019409165567815 26.489668237187146 26.89779792979182 23.59312466745321 56-57 23.089119136545722 26.712324214605815 26.70036019868447 23.498196450163995 58-59 23.08984976376967 26.600008223903266 26.926610973428254 23.383531038898802 60-61 23.07809495036375 26.55455928527498 27.03702333553756 23.330322428823706 62-63 23.080517913197543 26.536567830066954 26.92840498398372 23.454509272751782 64-65 22.933920814766605 26.536030715935816 26.81736473406346 23.712683735234123 66-67 22.93769942715927 26.65229082745984 26.76213672193129 23.647873023449602 68-69 22.894931820949243 26.556189155331012 26.869609500281715 23.67926952343803 70-71 22.768544632663875 26.465201139511972 26.792274811999324 23.97397941582483 72-73 22.947605699744315 26.600278287332173 26.603103539435473 23.849012473488035 74-75 22.86058013972031 26.834150935297934 26.42117568048637 23.88409324449539 76-77 22.85023911707199 26.649912849665608 26.480690371119703 24.0191576621427 78-79 22.723131882827435 26.72527764765709 26.486594298700815 24.064996170814652 80-81 22.774578762496077 26.796205222030938 26.428305780196954 24.00091023527603 82-83 22.962288167319738 26.717123817605508 26.400004991639005 23.920583023435746 84-85 22.91386835547552 26.728695690326543 26.252895535366168 24.104540418831775 86-87 22.725120921067482 26.785177469081862 26.276050587601986 24.213651022248676 88-89 22.69142582117885 26.69217337325011 26.230709581091904 24.385691224479135 90-91 22.706428412647856 26.628703331113694 26.280954177295072 24.383914078943373 92-93 22.723437678531837 26.439245639377607 26.3960133470244 24.441303335066156 94-95 22.588412887318913 26.34767297427036 26.43804854423113 24.6258655941796 96-97 22.609249589136105 26.25912821466445 26.299586371883336 24.832035824316108 98-99 22.40061821724286 26.32815878210601 26.272025619596747 24.99919738105438 100-101 22.649267206544838 26.41838565945886 26.085201836516664 24.847145297479635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25751.0 1 27739.0 2 20449.5 3 11939.0 4 15634.0 5 19947.0 6 21305.5 7 20567.5 8 18947.5 9 17094.5 10 15251.0 11 13473.5 12 11837.5 13 10300.0 14 8837.5 15 7805.5 16 7136.0 17 6652.0 18 6521.5 19 6573.5 20 6827.0 21 7503.5 22 8477.0 23 9589.5 24 11043.5 25 12943.0 26 15046.0 27 17312.5 28 19760.0 29 22538.0 30 25485.5 31 28692.5 32 32193.5 33 35015.0 34 37285.0 35 39736.5 36 42790.5 37 46179.0 38 49047.0 39 51180.5 40 53361.0 41 55712.5 42 58164.0 43 60864.0 44 63558.5 45 66257.0 46 68601.0 47 70688.0 48 71840.5 49 72137.5 50 71885.5 51 71024.5 52 69782.5 53 68381.5 54 66237.5 55 62107.0 56 56767.0 57 51274.0 58 46097.0 59 40578.5 60 34916.0 61 29720.0 62 24759.5 63 20101.0 64 15974.0 65 12446.5 66 9560.5 67 7539.0 68 5920.5 69 4572.0 70 3570.0 71 2767.0 72 2084.5 73 1627.0 74 1310.0 75 931.0 76 663.5 77 473.5 78 307.0 79 203.5 80 119.5 81 70.5 82 53.0 83 36.5 84 21.0 85 16.5 86 9.5 87 6.0 88 3.5 89 3.0 90 3.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.200114714983611 2 21.046108950465783 3 16.835997130327367 4 22.031974554130908 5 21.855406977475948 6 15.806259338572822 7 14.659379208538533 8 11.809709778283626 9 2.9587655373174315 10-11 6.377469833375588 12-13 3.473994041293798 14-15 7.250261165381968 16-17 11.263307747217082 18-19 14.2843079611839 20-21 14.719815844692455 22-23 12.637729901952852 24-25 15.140647222656748 26-27 12.72801401033154 28-29 12.736509751672628 30-31 13.555966707482186 32-33 12.068515232100058 34-35 18.895270434280718 36-37 23.627870346895953 38-39 22.71974751915363 40-41 17.82620050668781 42-43 17.744322237625436 44-45 23.0831764543001 46-47 12.513238073147884 48-49 21.23813967538177 50-51 20.907547454783774 52-53 10.975059380287362 54-55 12.560616440203468 56-57 12.0818881582851 58-59 13.092229588194785 60-61 12.513687583271752 62-63 12.469703017651364 64-65 13.528097079802432 66-67 11.854256231558848 68-69 12.87900446093847 70-71 11.52692295935889 72-73 10.901519524022719 74-75 10.979689334563194 76-77 11.03963150958086 78-79 11.428839850331109 80-81 11.405195617815703 82-83 9.947389335102605 84-85 9.842923182315912 86-87 9.983529949061513 88-89 11.36698725728701 90-91 8.902076017556066 92-93 10.892956356163054 94-95 10.993826427958812 96-97 10.560521144057207 98-99 10.391325533433664 100-101 10.505096545784404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2224644.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.10274281216407 #Duplication Level Percentage of deduplicated Percentage of total 1 98.89901414787107 96.033655351773 2 0.7604505134228849 1.4768366125256103 3 0.16506893335965875 0.48085938546903584 4 0.06140981432971662 0.2385224562800488 5 0.03372021591452789 0.16371627267595218 6 0.019980722273377376 0.11641097616678249 7 0.014174424298103736 0.09634628339904794 8 0.008352486446395642 0.06488394745971537 9 0.006232871494267039 0.05447060259201728 >10 0.028116916797101932 0.4777648262647089 >50 0.0018512968095263575 0.1283981174967435 >100 0.001441952907571973 0.2588453339953587 >500 4.642601892809951E-5 0.04363834774943334 >1k 1.3927805678429854E-4 0.36565148615253495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4115 0.1849734159712745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3485303716010292E-4 2 0.0 0.0 0.0 0.0 1.3485303716010292E-4 3 0.0 0.0 0.0 0.0 1.3485303716010292E-4 4 0.0 0.0 0.0 0.0 1.3485303716010292E-4 5 0.0 0.0 0.0 0.0 1.3485303716010292E-4 6 0.0 0.0 0.0 0.0 1.798040495468039E-4 7 0.0 0.0 0.0 0.0 1.798040495468039E-4 8 0.0 0.0 0.0 0.0 1.798040495468039E-4 9 0.0 0.0 0.0 0.0 1.798040495468039E-4 10-11 0.0 0.0 0.0 0.0 1.798040495468039E-4 12-13 0.0 2.2475506193350488E-5 0.0 0.0 3.5960809909360775E-4 14-15 0.0 4.4951012386700976E-5 0.0 0.0 4.7198563006036024E-4 16-17 0.0 4.4951012386700976E-5 0.0 0.0 6.068386672204631E-4 18-19 0.0 4.4951012386700976E-5 0.0 0.0 6.742651858005146E-4 20-21 0.0 4.4951012386700976E-5 0.0 2.2475506193350488E-5 6.967406919938652E-4 22-23 0.0 4.4951012386700976E-5 0.0 8.990202477340195E-5 7.192161981872156E-4 24-25 0.0 6.742651858005146E-5 0.0 3.8208360528695827E-4 7.192161981872156E-4 26-27 0.0 8.990202477340195E-5 0.0 7.86642716767267E-4 7.641672105739165E-4 28-29 0.0 8.990202477340195E-5 0.0 0.0021126975821749456 7.641672105739165E-4 30-31 0.0 8.990202477340195E-5 0.0 0.007147210969485455 7.641672105739165E-4 32-33 2.2475506193350488E-5 8.990202477340195E-5 0.0 0.018856949696221058 7.641672105739165E-4 34-35 4.4951012386700976E-5 8.990202477340195E-5 0.0 0.03623051598368099 7.641672105739165E-4 36-37 4.4951012386700976E-5 8.990202477340195E-5 0.0 0.05803175699123096 7.641672105739165E-4 38-39 4.4951012386700976E-5 8.990202477340195E-5 0.0 0.08603623770814567 7.641672105739165E-4 40-41 4.4951012386700976E-5 8.990202477340195E-5 0.0 0.1156589548709816 8.091182229606175E-4 42-43 4.4951012386700976E-5 1.3485303716010292E-4 0.0 0.1497542977662943 8.76544741540669E-4 44-45 4.4951012386700976E-5 1.3485303716010292E-4 0.0 0.19929031341643877 9.2149575392737E-4 46-47 4.4951012386700976E-5 1.3485303716010292E-4 0.0 0.25118625721688503 9.439712601207205E-4 48-49 4.4951012386700976E-5 1.573285433534534E-4 0.0 0.3002727627431625 9.439712601207205E-4 50-51 4.4951012386700976E-5 1.798040495468039E-4 0.0 0.3556524100035781 9.439712601207205E-4 52-53 4.4951012386700976E-5 1.798040495468039E-4 0.0 0.43143981688755595 0.0010788242972808234 54-55 4.4951012386700976E-5 2.022795557401544E-4 0.0 0.5272304242836157 0.0011237753096675243 56-57 4.4951012386700976E-5 2.2475506193350486E-4 0.0 0.6343037357887374 0.0012136773344409262 58-59 4.4951012386700976E-5 2.2475506193350486E-4 0.0 0.7422535920353999 0.0012811038530209777 60-61 4.4951012386700976E-5 2.2475506193350486E-4 0.0 0.8502259237882556 0.0013260548654076788 62-63 4.4951012386700976E-5 2.2475506193350486E-4 0.0 0.9633676219655819 0.0013485303716010292 64-65 4.4951012386700976E-5 2.2475506193350486E-4 0.0 1.0962652900868632 0.0013485303716010292 66-67 4.4951012386700976E-5 2.6970607432020584E-4 0.0 1.2493684382759667 0.0013485303716010292 68-69 4.4951012386700976E-5 2.6970607432020584E-4 0.0 1.402831194564164 0.0013485303716010292 70-71 4.4951012386700976E-5 2.6970607432020584E-4 0.0 1.5581594178664093 0.00139348138398773 72-73 4.4951012386700976E-5 2.6970607432020584E-4 0.0 1.7202752440390463 0.0015508099273411837 74-75 4.4951012386700976E-5 2.6970607432020584E-4 0.0 1.9057206456403812 0.001573285433534534 76-77 4.4951012386700976E-5 2.9218158051355636E-4 0.0 2.117012879364069 0.001573285433534534 78-79 4.4951012386700976E-5 3.146570867069068E-4 0.0 2.3425321085081476 0.001573285433534534 80-81 4.4951012386700976E-5 3.146570867069068E-4 0.0 2.5702089862467883 0.0015957609397278844 82-83 4.4951012386700976E-5 3.146570867069068E-4 0.0 2.8068311154503824 0.001618236445921235 84-85 4.4951012386700976E-5 3.146570867069068E-4 0.0 3.063141788079351 0.001618236445921235 86-87 4.4951012386700976E-5 3.146570867069068E-4 0.0 3.348827947303029 0.0016407119521145855 88-89 6.742651858005146E-5 3.596080990936078E-4 0.0 3.6453922515242887 0.001663187458307936 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 63.49112 1 GTATCAA 5445 0.0 45.35816 1 GTGGTAT 1085 0.0 39.405876 1 TATCAAC 6710 0.0 36.867615 2 ATCAACG 6820 0.0 36.85836 3 TCAACGC 6910 0.0 36.43576 4 CAACGCA 7220 0.0 34.183525 5 TGGTATC 1315 0.0 31.73619 2 AACGCAG 7725 0.0 31.350245 6 ACGCAGA 8850 0.0 27.27158 7 GTACATG 6585 0.0 26.888012 1 TACATGG 6665 0.0 26.026838 2 CGCAGAG 9325 0.0 25.714838 8 ACATGGG 7130 0.0 24.185495 3 GCAGAGT 9870 0.0 22.719618 9 CATGGGG 5210 0.0 21.895573 4 GAGTACT 5730 0.0 21.446291 12-13 CAGAGTA 9360 0.0 20.973011 10-11 GTACTTT 6245 0.0 19.714218 14-15 AGAGTAC 8815 0.0 18.855179 10-11 >>END_MODULE