FastQCFastQC Report
Sat 18 Jun 2016
SRR1930967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224644
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56300.2530741997371265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.23927423893440927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.13898853029967942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.065.950791
GTATCAA70150.041.639991
GTGGTAT13400.032.9641081
TATCAAC93400.031.0634772
ATCAACG94950.030.6063923
TCAACGC95500.030.5295724
CAACGCA96000.030.3013365
AACGCAG100350.029.129696
TGGTATC14750.028.0080532
ACGCAGA115400.025.2073527
CGCAGAG119750.024.093548
GTACATG86100.023.3897671
TACATGG87900.022.7430612
GCAGAGT127750.021.8789739
ACATGGG90100.021.8188193
GAGTACT75750.021.76931412-13
CAGAGTA122650.021.27980610-11
CATGGGG64250.020.1764724
TAACGCA1908.2796305E-619.993654
GTACTTT82550.019.7461414-15
Produced by FastQC (version 0.11.5)