##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930967_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2224644 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27937099149347 31.0 31.0 33.0 30.0 34.0 2 31.389987791305035 31.0 31.0 34.0 30.0 34.0 3 31.454198963969066 31.0 31.0 34.0 30.0 34.0 4 35.33661700478818 37.0 35.0 37.0 33.0 37.0 5 35.128956363355215 37.0 35.0 37.0 32.0 37.0 6 35.173858379138416 37.0 35.0 37.0 32.0 37.0 7 35.05191482322565 37.0 35.0 37.0 32.0 37.0 8 35.03151380625395 37.0 35.0 37.0 32.0 37.0 9 36.53803664766138 38.0 35.0 39.0 32.0 39.0 10-11 36.47282396644137 38.0 35.0 39.0 32.0 39.0 12-13 36.36026775520038 38.0 35.0 39.0 32.0 39.0 14-15 37.36302797211599 39.0 36.0 41.0 32.0 41.0 16-17 37.31982757690669 39.0 36.0 40.5 32.0 41.0 18-19 37.32222121831628 39.0 36.0 40.0 32.0 41.0 20-21 37.29045815869865 39.0 36.0 40.0 31.5 41.0 22-23 37.156124755241734 39.0 36.0 40.0 31.0 41.0 24-25 37.0230207619736 39.0 36.0 40.0 31.0 41.0 26-27 36.738936656831385 38.5 35.5 40.0 30.5 41.0 28-29 36.53915323080906 38.0 35.0 40.0 30.0 41.0 30-31 36.25667904617548 38.0 35.0 40.0 30.0 41.0 32-33 35.96127964744022 38.0 34.5 40.0 29.5 41.0 34-35 36.120971040759784 38.0 35.0 40.0 30.0 41.0 36-37 36.14476406112618 38.0 35.0 40.0 30.0 41.0 38-39 36.072039616226235 38.0 35.0 40.0 30.0 41.0 40-41 35.93690675901403 38.0 35.0 40.0 29.0 41.0 42-43 35.73946955108323 38.0 34.5 40.0 28.5 41.0 44-45 35.488782699613964 38.0 34.0 40.0 27.5 41.0 46-47 35.18484845215684 38.0 34.0 40.0 27.0 41.0 48-49 34.913957244395064 38.0 33.5 40.0 26.0 41.0 50-51 34.58797677291288 38.0 33.0 40.0 25.0 41.0 52-53 34.337151022815334 37.5 33.0 40.0 24.0 41.0 54-55 34.12574146694932 37.0 33.0 40.0 24.0 41.0 56-57 33.834297083038905 37.0 33.0 40.0 23.5 41.0 58-59 33.555917935633744 36.5 32.0 39.5 23.0 41.0 60-61 33.21349842941163 36.0 32.0 39.0 22.5 41.0 62-63 32.87690614768026 35.5 31.0 39.0 22.0 40.5 64-65 32.539952909319425 35.0 31.0 39.0 21.5 40.0 66-67 32.17333627312954 35.0 31.0 38.0 20.5 40.0 68-69 31.792605693315423 35.0 31.0 38.0 20.0 40.0 70-71 31.38161791279863 34.5 30.0 37.0 18.5 39.5 72-73 30.95361909590928 34.0 30.0 36.5 18.0 39.0 74-75 30.44032415973073 34.0 29.0 36.0 14.0 39.0 76-77 29.11365234167804 32.5 28.0 35.0 12.0 37.0 78-79 29.86902983128986 34.0 29.0 35.0 13.0 37.0 80-81 29.892004293720703 34.0 29.0 35.0 11.5 37.0 82-83 29.64928051409574 34.0 29.0 35.0 9.0 36.5 84-85 29.35559667074822 34.0 29.0 35.0 7.0 36.0 86-87 29.03559558293372 34.0 29.0 35.0 4.5 36.0 88-89 28.71542772686326 33.5 29.0 35.0 2.0 35.0 90-91 28.393677145646677 33.0 28.5 35.0 2.0 35.0 92-93 28.050920731586714 33.0 27.0 35.0 2.0 35.0 94-95 27.6401273641985 33.0 27.0 35.0 2.0 35.0 96-97 27.176354059346124 33.0 26.0 35.0 2.0 35.0 98-99 26.681922141250467 33.0 25.0 35.0 2.0 35.0 100-101 25.48407070974052 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47239428279026185 1101 2 0.5092094409379939 1101 3 0.5028789498688049 1101 4 0.3326425288318049 1101 5 0.4858831635745986 1101 6 0.4339393301822767 1101 7 0.4504162119696531 1101 8 0.4428520947498953 1101 9 0.6592047070282163 1101 10-11 0.6233789696341461 1101 12-13 0.608181922267697 1101 14-15 0.77481100022748 1101 16-17 0.70201387367009 1101 18-19 0.6765797142731671 1101 20-21 0.7663477348709051 1101 22-23 0.793102295633517 1101 24-25 0.678963684884323 1101 26-27 0.6195900949780793 1101 28-29 0.5883108384026698 1101 30-31 0.7500914621530299 1101 32-33 0.6763203773009181 1101 34-35 0.4803232916811524 1101 36-37 0.7602831637849121 1101 38-39 0.9266725518679806 1101 40-41 0.82514073980213 1101 42-43 0.875399497696371 1101 44-45 0.7912385866630274 1101 46-47 0.7695832410723611 1101 48-49 0.8342223195319676 1101 50-51 0.8528701141364508 1101 52-53 0.8703092980588565 1101 54-55 0.9255679145410767 1101 56-57 1.042160672451864 1101 58-59 0.8397972590159526 1101 60-61 0.8095784214425663 1101 62-63 0.8092073968654674 1101 64-65 0.7721573667895072 1101 66-67 0.7899838963660102 1101 68-69 0.7256343458053749 1101 70-71 0.6074634354899864 1101 72-73 0.6518461478635125 1101 74-75 0.5650894541567766 1101 76-77 0.7227025561249469 1101 78-79 0.7664469077099838 1101 80-81 0.5502564564053181 1101 82-83 0.6731470267362454 1101 84-85 0.8997296739757097 1101 86-87 0.6600761528854804 1101 88-89 0.661027765873623 1101 90-91 0.41199667401365403 1101 92-93 0.6615085216057821 1101 94-95 0.6852462813760383 1101 96-97 0.7148608063937019 1101 98-99 0.7895030727909109 1101 100-101 1.022557084383596 1102 1 0.3883372601282389 1102 2 0.5090818893822586 1102 3 0.5288863288814092 1102 4 0.3165965796155774 1102 5 0.4236883680795245 1102 6 0.2820454997908115 1102 7 0.4704936297422435 1102 8 0.4724851130485277 1102 9 0.6531986528000076 1102 10-11 0.5578038754348569 1102 12-13 0.6016666654410869 1102 14-15 0.7631681343648253 1102 16-17 0.5811963649107312 1102 18-19 0.49606424540807126 1102 20-21 0.6855166352104405 1102 22-23 0.6982790275290895 1102 24-25 0.7714062643307855 1102 26-27 0.7521445365914943 1102 28-29 0.8179654984960223 1102 30-31 0.8719713531119595 1102 32-33 0.8540168472979204 1102 34-35 0.7596248857836301 1102 36-37 0.774603061261935 1102 38-39 0.8206857854787444 1102 40-41 0.7018116150010556 1102 42-43 0.766457969409764 1102 44-45 0.9218100486538532 1102 46-47 0.9693389223628088 1102 48-49 0.8478251728912909 1102 50-51 0.9459686990805878 1102 52-53 0.8815394917565698 1102 54-55 0.901742324049458 1102 56-57 0.8733121564384874 1102 58-59 0.8953169476660676 1102 60-61 0.8111601337245773 1102 62-63 0.7193777083011028 1102 64-65 0.765474960856956 1102 66-67 0.7566329965618621 1102 68-69 0.6723705314153392 1102 70-71 0.6633932416243944 1102 72-73 0.7339044978646143 1102 74-75 0.7968422369795434 1102 76-77 0.9702054926391241 1102 78-79 0.9548951162127963 1102 80-81 0.7004635588919861 1102 82-83 0.9093087321826623 1102 84-85 0.9051196852108276 1102 86-87 0.9352881594466282 1102 88-89 0.9290967940749262 1102 90-91 0.8858741518942246 1102 92-93 0.981561377948605 1102 94-95 0.9103714926618132 1102 96-97 0.8995247116938785 1102 98-99 1.0047073642164754 1102 100-101 1.1492387713560213 1103 1 0.44941593086555187 1103 2 0.5672823051201661 1103 3 0.4731789311751946 1103 4 0.28285527816511546 1103 5 0.4298684219115074 1103 6 0.4017089197699377 1103 7 0.4520029780629571 1103 8 0.41232327738353547 1103 9 0.5031842696561881 1103 10-11 0.6152058098592903 1103 12-13 0.4900360325249409 1103 14-15 0.6363012167520523 1103 16-17 0.6206819795985936 1103 18-19 0.6020541923855873 1103 20-21 0.6280966315098482 1103 22-23 0.6880101675414068 1103 24-25 0.7317715781146035 1103 26-27 0.8010352095202435 1103 28-29 0.8408561996128512 1103 30-31 0.8613889008365376 1103 32-33 0.8677488647542901 1103 34-35 0.7555238828665267 1103 36-37 0.8542807993354842 1103 38-39 0.7715227182118838 1103 40-41 1.000329428416272 1103 42-43 1.0653387945830062 1103 44-45 1.0531415230200452 1103 46-47 0.9766247255493639 1103 48-49 0.9342377819213041 1103 50-51 1.0956316348031123 1103 52-53 0.8446355248999637 1103 54-55 0.9978305787308415 1103 56-57 1.1733448979668921 1103 58-59 1.2233036725302568 1103 60-61 0.9712422658124282 1103 62-63 0.8807689594466979 1103 64-65 0.6880065531793917 1103 66-67 0.6415921491561747 1103 68-69 0.7197601895735417 1103 70-71 0.7305832125979101 1103 72-73 0.6565923894258319 1103 74-75 0.9011018700720506 1103 76-77 0.9715087391821093 1103 78-79 1.139119608177026 1103 80-81 1.0278740545394633 1103 82-83 0.9856352519533402 1103 84-85 1.052750188750963 1103 86-87 1.0362400308454198 1103 88-89 1.039475677885143 1103 90-91 1.184234401221321 1103 92-93 1.090194046910792 1103 94-95 1.1433767963557528 1103 96-97 1.1374324810489647 1103 98-99 1.2107693895365905 1103 100-101 1.4823942126655645 1104 1 0.359827034726532 1104 2 0.37685762872878215 1104 3 0.2985839248933395 1104 4 0.21715568111973482 1104 5 0.29669986210222987 1104 6 0.31706412369416626 1104 7 0.46113742696241644 1104 8 0.45776710699783507 1104 9 0.4163504827127156 1104 10-11 0.40163038179156274 1104 12-13 0.44509366032995246 1104 14-15 0.5220590747964025 1104 16-17 0.4771223312967052 1104 18-19 0.5934893497468039 1104 20-21 0.4628596595649981 1104 22-23 0.46611941818318314 1104 24-25 0.510909321769077 1104 26-27 0.6601038527537284 1104 28-29 0.4973368503502158 1104 30-31 0.5235734843880877 1104 32-33 0.6499171748633543 1104 34-35 0.5627352823611176 1104 36-37 0.4274642481462294 1104 38-39 0.5426701443678752 1104 40-41 0.5151336462750535 1104 42-43 0.509947738286705 1104 44-45 0.43699765276686264 1104 46-47 0.4275766092477298 1104 48-49 0.44297916762156575 1104 50-51 0.4620926386409181 1104 52-53 0.40200862138298277 1104 54-55 0.47263428860351553 1104 56-57 0.3403932696925196 1104 58-59 0.44003943604054285 1104 60-61 0.3221756187497391 1104 62-63 0.377891235997204 1104 64-65 0.47558715125791906 1104 66-67 0.45176373040962403 1104 68-69 0.47020333591075314 1104 70-71 0.38920751107623985 1104 72-73 0.5078270460987646 1104 74-75 0.6907220145118202 1104 76-77 0.6653819079279408 1104 78-79 0.606933768969661 1104 80-81 0.6065772911980254 1104 82-83 0.6672054978472381 1104 84-85 0.5946667345026242 1104 86-87 0.6231168356270729 1104 88-89 0.5653327076974897 1104 90-91 0.4604053449879757 1104 92-93 0.3517351694599391 1104 94-95 0.5207276630802262 1104 96-97 0.5234549748171915 1104 98-99 0.49448893541131156 1104 100-101 0.7409685353268962 1105 1 0.17225845983693944 1105 2 0.2829268543971608 1105 3 0.3286488829750098 1105 4 0.21717611705344098 1105 5 0.1629086229165324 1105 6 0.27421772228591834 1105 7 0.2917459104117768 1105 8 0.29891824200255 1105 9 0.4267262995334633 1105 10-11 0.40356298690096537 1105 12-13 0.2902904936058519 1105 14-15 0.4405905204132168 1105 16-17 0.2717508870610743 1105 18-19 0.3586721033690736 1105 20-21 0.39042229041663035 1105 22-23 0.4371117554127366 1105 24-25 0.47460030508278805 1105 26-27 0.4474208366354091 1105 28-29 0.45893596920912927 1105 30-31 0.3760113327238557 1105 32-33 0.4463871668087833 1105 34-35 0.44747855418228966 1105 36-37 0.47536880740303644 1105 38-39 0.40175936060754225 1105 40-41 0.5981740939206546 1105 42-43 0.5380787699701486 1105 44-45 0.3963883694973376 1105 46-47 0.4593861089972151 1105 48-49 0.49125650649655483 1105 50-51 0.6556618026491421 1105 52-53 0.5927767411854674 1105 54-55 0.6096631258200276 1105 56-57 0.4925771134661474 1105 58-59 0.33360539372124265 1105 60-61 0.4822400694565019 1105 62-63 0.4569288832152054 1105 64-65 0.5515718285357636 1105 66-67 0.4302772840141387 1105 68-69 0.45058801065172105 1105 70-71 0.41189474474845866 1105 72-73 0.3986167117175583 1105 74-75 0.40293394712397657 1105 76-77 0.3936630908725327 1105 78-79 0.5391245222029397 1105 80-81 0.5761201745955233 1105 82-83 0.5570477993843745 1105 84-85 0.5198839015325589 1105 86-87 0.42864743350497747 1105 88-89 0.5084980858432999 1105 90-91 0.5477184883235005 1105 92-93 0.5884955031716892 1105 94-95 0.3326928494101402 1105 96-97 0.30883477312157126 1105 98-99 0.5455256538513602 1105 100-101 0.7950277459783912 1106 1 0.12153758009972293 1106 2 0.11165072955189714 1106 3 0.15178298461437834 1106 4 0.04626479667091843 1106 5 0.04331049549925581 1106 6 0.0556759641174267 1106 7 0.10002217753593357 1106 8 0.01990142353736246 1106 9 0.18296543530258447 1106 10-11 0.14385829465295785 1106 12-13 0.19405110695848293 1106 14-15 0.22391625866540465 1106 16-17 0.2555525722755547 1106 18-19 0.21119684924315862 1106 20-21 0.1710953348894364 1106 22-23 0.38551666808992024 1106 24-25 0.36662402648008197 1106 26-27 0.4600918204699198 1106 28-29 0.4238456633507397 1106 30-31 0.3060107175566529 1106 32-33 0.21018950843072304 1106 34-35 0.3563532174727513 1106 36-37 0.4271384097575819 1106 38-39 0.3026508547352691 1106 40-41 0.34027392078030516 1106 42-43 0.22530619762957826 1106 44-45 0.23617331185376145 1106 46-47 0.19853811646994757 1106 48-49 0.324285435163965 1106 50-51 0.18412754814588084 1106 52-53 0.24122062590794258 1106 54-55 0.32645947591959157 1106 56-57 0.5834604214023571 1106 58-59 0.4210940268747052 1106 60-61 0.3291713473753006 1106 62-63 0.42465193631409903 1106 64-65 0.4245961623509231 1106 66-67 0.38051077261704336 1106 68-69 0.25539064507950826 1106 70-71 0.43377149960742045 1106 72-73 0.44724000875259406 1106 74-75 0.573466286704523 1106 76-77 0.5020151713405276 1106 78-79 0.4327923552816131 1106 80-81 0.38723548992198786 1106 82-83 0.42831658690012375 1106 84-85 0.30886883440372515 1106 86-87 0.38528433157761555 1106 88-89 0.3144741774901547 1106 90-91 0.2837248949978246 1106 92-93 0.3117463834793952 1106 94-95 0.4939201835916478 1106 96-97 0.4180055586591642 1106 98-99 0.442433167070579 1106 100-101 0.6349366668651371 1107 1 0.13994566127623287 1107 2 0.1583295882309379 1107 3 0.19507256517200133 1107 4 -0.08929450767514169 1107 5 0.09519764001850461 1107 6 0.13738541258928905 1107 7 0.14528004197494226 1107 8 0.026620539498260598 1107 9 0.10184262188596449 1107 10-11 0.12952406802675398 1107 12-13 0.12831446118035217 1107 14-15 0.18173037607009945 1107 16-17 0.22975489888977307 1107 18-19 0.2628529872121561 1107 20-21 0.3112336517500438 1107 22-23 0.298785866709963 1107 24-25 0.2539166398429984 1107 26-27 0.31910073820085216 1107 28-29 0.20969490793568468 1107 30-31 0.27712544236672443 1107 32-33 0.4077521191424651 1107 34-35 0.19139773858164943 1107 36-37 0.13305693942114516 1107 38-39 0.22444221934567565 1107 40-41 0.15128894802658266 1107 42-43 0.26748144176534083 1107 44-45 0.27092308008732857 1107 46-47 0.1261339428165158 1107 48-49 0.18067737627756486 1107 50-51 0.19203606077103075 1107 52-53 0.16032797471938665 1107 54-55 0.2433195767992018 1107 56-57 0.29260037793665816 1107 58-59 0.12177463498931473 1107 60-61 0.21897311741232528 1107 62-63 0.09299863526764085 1107 64-65 0.03330973618098909 1107 66-67 0.13064848156568587 1107 68-69 -0.051891464915414076 1107 70-71 0.03072966006578781 1107 72-73 -0.030074067280310146 1107 74-75 0.17139507994859216 1107 76-77 0.26217126962638915 1107 78-79 0.31053610687527566 1107 80-81 0.30628643801136946 1107 82-83 0.31875809985793424 1107 84-85 0.35327823947742587 1107 86-87 0.21233025088405455 1107 88-89 0.3259867638009908 1107 90-91 0.39339077982214476 1107 92-93 0.24768858793707693 1107 94-95 0.3718148999922697 1107 96-97 0.4482701185731095 1107 98-99 0.27048636274562554 1107 100-101 0.6330729100704531 1108 1 0.26516656222177204 1108 2 0.3144298263360099 1108 3 0.346163994765849 1108 4 0.14770949744480077 1108 5 0.21512483216792333 1108 6 0.1752368928320749 1108 7 0.23182744965761515 1108 8 0.2536409917602569 1108 9 0.3131147635928926 1108 10-11 0.2750427379415612 1108 12-13 0.2844236454296336 1108 14-15 0.3893172224854453 1108 16-17 0.3696844652082234 1108 18-19 0.4466303589726195 1108 20-21 0.3826412587252861 1108 22-23 0.4775189894648264 1108 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-0.7166266516801798 2316 62-63 -0.9158843060040098 2316 64-65 -0.7583732208343577 2316 66-67 -0.5591642559563503 2316 68-69 -0.6055868594479783 2316 70-71 -0.692073110824122 2316 72-73 -0.7109601310510101 2316 74-75 -0.2661293555744244 2316 76-77 -0.7483247441580936 2316 78-79 -0.6564659879105008 2316 80-81 -0.648777783135035 2316 82-83 -0.915129105330486 2316 84-85 -0.8616758632851571 2316 86-87 -0.5872009369338258 2316 88-89 -0.7920273005644702 2316 90-91 -0.47432918361750254 2316 92-93 -0.6871944058135497 2316 94-95 -0.5724067274682305 2316 96-97 -0.3418017338909962 2316 98-99 -0.5195417572388088 2316 100-101 -0.7190223130307913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 52.0 5 103.0 6 222.0 7 561.0 8 1254.0 9 2310.0 10 3837.0 11 5865.0 12 8179.0 13 10305.0 14 12035.0 15 14638.0 16 17373.0 17 19976.0 18 17324.0 19 14216.0 20 13706.0 21 14474.0 22 16185.0 23 18497.0 24 22646.0 25 27894.0 26 34639.0 27 44089.0 28 55661.0 29 68152.0 30 83341.0 31 100192.0 32 121345.0 33 147021.0 34 181426.0 35 226425.0 36 290942.0 37 349922.0 38 246939.0 39 32863.0 40 11.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.3798044067821 19.684579859437605 14.058397416069003 23.877218317711293 2 17.29982387575824 21.21452004570779 37.287587781167204 24.198068297366767 3 19.42737004501528 25.076036893289423 29.99490240396156 25.50169065773374 4 14.283543042456543 17.584854177889575 35.43001986623343 32.70158291342046 5 14.404695868099976 36.83427178172736 33.713882173556165 15.047150176616498 6 32.15640988527592 35.53178443992337 17.134577158931638 15.177228515869071 7 27.74623715075311 32.19346556123137 21.4263046132325 18.63399267478302 8 26.599267118694048 33.83125569754082 19.962924404983447 19.606552778781683 9 26.496643957415213 15.303527216039962 19.95281042719644 38.24701839934839 10-11 25.613154284460794 25.402648693453873 27.489634296543624 21.494562725541705 12-13 26.2330287452734 23.429209347652925 27.537214943154947 22.800546963918723 14-15 23.498489420549724 24.91249157730027 25.421067560053455 26.167951442096555 16-17 22.92726386783683 27.57418715084301 26.409146811804497 23.08940216951566 18-19 22.850599916211312 26.05169636130545 28.614353577471274 22.483350145011965 20-21 23.718334259324187 25.132178451923092 28.58843931883034 22.56104796992238 22-23 23.63897855993556 25.09282948031898 28.322065984954442 22.946125974791016 24-25 23.21670947152797 25.556064254266047 28.36686811585115 22.86035815835484 26-27 22.959166013738244 25.808541157865733 28.487210919031032 22.745081909364995 28-29 22.95746911188407 25.868274535857903 28.531984022597793 22.642272329660234 30-31 23.448490247075 25.471181776108782 28.35964614754911 22.720681829267114 32-33 23.21526321693577 25.65426841646305 28.077829475557902 23.052638891043276 34-35 23.414514626232247 25.74106730415842 28.09762884987361 22.746789219735717 36-37 23.282342036511274 25.764240133631333 28.1721258565477 22.781291973309695 38-39 23.068541377947856 25.781670365414065 28.313150923727886 22.836637332910193 40-41 23.295740091935546 25.624763440831217 28.261662086072025 22.817834381161216 42-43 23.333863799631192 25.704958241513214 27.836331367011653 23.124846591843944 44-45 23.167909436282937 25.938865355161482 27.778069982607068 23.11515522594851 46-47 23.316531634956974 26.095925897205074 27.445074180341226 23.142468287496726 48-49 23.266752216691966 26.173047965781787 27.34008877332368 23.22011104420257 50-51 23.32323344919488 26.18588065472471 27.284347206318 23.20653868976241 52-53 23.505867067406232 26.21378238591301 27.10802586218334 23.17232468449742 54-55 23.194072595420018 26.244545902092618 27.138264894379894 23.42311660810747 56-57 23.256987503470008 26.47131215250734 26.975129444000505 23.29657090002214 58-59 23.10023815165184 26.43773325666202 27.257731161538935 23.204297430147204 60-61 23.184543787392748 26.523982518099746 27.201782821008415 23.089690873499087 62-63 23.14244254716026 26.46050011811156 27.134635935162375 23.2624213995658 64-65 22.989258285321394 26.43324855874195 27.032902800801306 23.54459035513534 66-67 22.926469052958986 26.626236135660825 26.95997476429273 23.487320047087454 68-69 22.94385969175276 26.527680468990106 27.062417568769753 23.46604227048738 70-71 22.955294579216897 26.398141090995473 27.036044369776935 23.61051996001069 72-73 23.01372457202645 26.53433751984285 26.865970069628215 23.585967838502484 74-75 23.006667629495826 26.75735285668483 26.615947650332593 23.620031863486744 76-77 22.916794407995585 26.72011213584704 26.596781639874052 23.766311816283324 78-79 22.79184118240189 26.78597196929272 26.694018266667086 23.7281685816383 80-81 22.84989284722948 26.86517180966252 26.612163159899804 23.6727721832082 82-83 22.95717876658018 26.781835655502633 26.59425508081293 23.666730497104254 84-85 22.948598281027948 26.725765274301256 26.43616831191155 23.889468132759248 86-87 22.77611608868655 26.85384717734613 26.44587178892443 23.924164945042893 88-89 22.683892840093534 26.84054664174576 26.423849633063895 24.05171088509681 90-91 22.680898753160257 26.74820993803563 26.528430607452268 24.042460701351853 92-93 22.768994974896962 26.58885107810198 26.594583391218457 24.0475705557826 94-95 22.5968125620334 26.515458004286597 26.62511777355831 24.262611660121696 96-97 22.57260038010576 26.446478627591652 26.553304708528646 24.427616283773943 98-99 22.396055278956993 26.564542461625322 26.484080149453128 24.55532210996456 100-101 22.372478472960168 26.61625860137622 26.44076984901854 24.57049307664507 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 154.0 1 123.5 2 149.0 3 205.5 4 201.5 5 227.5 6 324.0 7 654.5 8 1065.5 9 1343.5 10 1373.5 11 1257.5 12 1368.0 13 1599.5 14 1827.0 15 2055.5 16 2266.5 17 2574.5 18 2906.0 19 3193.5 20 3566.5 21 4118.0 22 4833.5 23 5669.0 24 6900.5 25 8637.5 26 10852.0 27 13340.5 28 15909.0 29 19159.0 30 22753.0 31 26791.5 32 31868.0 33 34741.5 34 36383.0 35 39374.0 36 43591.5 37 48245.5 38 51349.5 39 53364.5 40 55528.0 41 58648.0 42 61962.5 43 65959.0 44 69883.5 45 74264.5 46 78555.5 47 81599.0 48 84483.5 49 85951.0 50 86152.5 51 85806.5 52 85770.0 53 86326.0 54 85470.5 55 81365.5 56 74921.5 57 67727.0 58 61357.5 59 54671.0 60 46843.5 61 39671.0 62 33231.5 63 26478.5 64 20400.5 65 15811.5 66 12012.0 67 9366.5 68 7235.0 69 5396.0 70 4154.5 71 3097.0 72 2258.5 73 1785.0 74 1393.5 75 980.5 76 710.0 77 471.0 78 267.5 79 160.5 80 101.0 81 67.0 82 40.5 83 28.0 84 13.5 85 8.5 86 6.0 87 4.0 88 3.5 89 2.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019598641400601625 2 0.003506178966162676 3 0.0029667668175222643 4 0.03461227953775975 5 0.0022475506193350486 6 8.990202477340194E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.495101238670097E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2475506193350485E-5 24-25 6.068386672204632E-4 26-27 0.0022925016317217497 28-29 8.990202477340194E-5 30-31 0.007079784450905403 32-33 0.022453030687157136 34-35 0.013552730234590344 36-37 7.192161981872155E-4 38-39 7.641672105739165E-4 40-41 0.001887942520241441 42-43 0.008360888303926381 44-45 0.007956329192446071 46-47 0.007484343562385713 48-49 0.00964199215694736 50-51 0.19425580002912826 52-53 0.048929176982924004 54-55 0.017935453942293688 56-57 0.01018140430558777 58-59 0.01937388633866812 60-61 0.054008641382621216 62-63 0.09749874586675442 64-65 0.10248830824167822 66-67 0.10770262567853554 68-69 0.10505051594772018 70-71 0.092149575392737 72-73 0.06971902021177322 74-75 0.246758582496795 76-77 0.2975757019999604 78-79 0.006967406919938651 80-81 7.416917043805661E-4 82-83 0.0 84-85 2.2475506193350485E-5 86-87 0.0 88-89 2.6970607432020584E-4 90-91 0.006293141734138137 92-93 0.0184748660909341 94-95 0.0016182364459212352 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2224644.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.403235062469896 #Duplication Level Percentage of deduplicated Percentage of total 1 80.66697156804922 50.33879988538552 2 11.084730355487308 13.8344606795514 3 3.3114667900706043 6.199387215070158 4 1.5094957947699736 3.7678968362736187 5 0.8623592569260541 2.6907003709126713 6 0.5643819387376553 2.1131555272835034 7 0.36513872692412236 1.595008646465991 8 0.2898653009439855 1.4470826009008884 9 0.20539314026274208 1.1535476770831272 >10 1.0742013597203492 11.615064987154962 >50 0.04606296255782965 1.9384017763325643 >100 0.01856231846759587 2.1103445241391663 >500 9.37701688020892E-4 0.37742574162146636 >1k 2.8852359631407847E-4 0.32570693695558606 >5k 1.4426179815703923E-4 0.49301659486933863 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5630 0.2530741997371265 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5323 0.23927423893440927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3092 0.13898853029967942 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4951012386700976E-5 2 0.0 0.0 0.0 0.0 4.4951012386700976E-5 3 0.0 0.0 0.0 0.0 4.4951012386700976E-5 4 0.0 0.0 0.0 0.0 4.4951012386700976E-5 5 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 6 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 7 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 8 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 9 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 10-11 0.0 8.990202477340195E-5 0.0 0.0 4.4951012386700976E-5 12-13 0.0 8.990202477340195E-5 0.0 4.4951012386700976E-5 2.9218158051355636E-4 14-15 0.0 8.990202477340195E-5 0.0 8.990202477340195E-5 4.045591114803088E-4 16-17 0.0 8.990202477340195E-5 0.0 8.990202477340195E-5 4.944611362537108E-4 18-19 0.0 8.990202477340195E-5 0.0 8.990202477340195E-5 5.394121486404117E-4 20-21 0.0 8.990202477340195E-5 0.0 1.573285433534534E-4 5.843631610271126E-4 22-23 0.0 8.990202477340195E-5 0.0 3.596080990936078E-4 6.293141734138136E-4 24-25 0.0 8.990202477340195E-5 0.0 8.540692353473185E-4 6.742651858005146E-4 26-27 0.0 8.990202477340195E-5 0.0 0.0014384323963744312 7.86642716767267E-4 28-29 0.0 8.990202477340195E-5 0.0 0.00415796864576984 8.091182229606175E-4 30-31 0.0 8.990202477340195E-5 0.0 0.013687583271750445 8.091182229606175E-4 32-33 0.0 8.990202477340195E-5 0.0 0.039399562356943404 8.540692353473185E-4 34-35 0.0 8.990202477340195E-5 0.0 0.0785294186395666 8.990202477340194E-4 36-37 0.0 1.1237753096675243E-4 0.0 0.12761592416584405 9.2149575392737E-4 38-39 0.0 1.3485303716010292E-4 0.0 0.18868187449317733 9.439712601207205E-4 40-41 0.0 1.3485303716010292E-4 0.0 0.25336638131764 9.439712601207205E-4 42-43 0.0 1.3485303716010292E-4 0.0 0.3279176353609836 9.439712601207205E-4 44-45 0.0 1.3485303716010292E-4 0.0 0.4304958456274352 9.439712601207205E-4 46-47 0.0 1.3485303716010292E-4 0.0 0.5371421225148834 9.439712601207205E-4 48-49 0.0 1.3485303716010292E-4 0.0 0.6349105744559579 0.0010788242972808234 50-51 0.0 1.3485303716010292E-4 0.0 0.7351063810659144 0.0010788242972808234 52-53 0.0 1.3485303716010292E-4 0.0 0.8419324620029092 0.0011687263220542254 54-55 0.0 1.3485303716010292E-4 0.0 0.9759763809400516 0.0012586283468276273 56-57 0.0 1.3485303716010292E-4 0.0 1.126180188830213 0.0013035793592143282 58-59 0.0 1.3485303716010292E-4 0.0 1.275799633559347 0.0013485303716010292 60-61 0.0 1.3485303716010292E-4 0.0 1.4269923637220159 0.0013485303716010292 62-63 0.0 1.3485303716010292E-4 0.0 1.5869730168062843 0.0013485303716010292 64-65 0.0 1.3485303716010292E-4 0.0 1.7755200382623018 0.0013485303716010292 66-67 0.0 1.3485303716010292E-4 0.0 1.996386837624357 0.0013485303716010292 68-69 0.0 1.3485303716010292E-4 0.0 2.2216363606941156 0.0013710058777943795 70-71 0.0 1.3485303716010292E-4 0.0 2.45027968519907 0.0014159568901810807 72-73 0.0 1.3485303716010292E-4 0.0 2.693397235692542 0.001573285433534534 74-75 0.0 1.3485303716010292E-4 0.0 2.9711495412299676 0.001573285433534534 76-77 0.0 1.3485303716010292E-4 0.0 3.2889531988039433 0.001573285433534534 78-79 0.0 1.3485303716010292E-4 0.0 3.625771134617494 0.0015957609397278844 80-81 0.0 1.3485303716010292E-4 0.0 3.966926843126361 0.0016631874583079359 82-83 0.0 1.3485303716010292E-4 0.0 4.327860997085376 0.0018429915078547398 84-85 0.0 1.3485303716010292E-4 0.0 4.728509370488043 0.0018429915078547398 86-87 0.0 1.3485303716010292E-4 0.0 5.181952707938889 0.0018879425202414407 88-89 0.0 1.3485303716010292E-4 0.0 5.6610630734625405 0.0019328935326281419 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3925 0.0 65.95079 1 GTATCAA 7015 0.0 41.63999 1 GTGGTAT 1340 0.0 32.964108 1 TATCAAC 9340 0.0 31.063477 2 ATCAACG 9495 0.0 30.606392 3 TCAACGC 9550 0.0 30.529572 4 CAACGCA 9600 0.0 30.301336 5 AACGCAG 10035 0.0 29.12969 6 TGGTATC 1475 0.0 28.008053 2 ACGCAGA 11540 0.0 25.207352 7 CGCAGAG 11975 0.0 24.09354 8 GTACATG 8610 0.0 23.389767 1 TACATGG 8790 0.0 22.743061 2 GCAGAGT 12775 0.0 21.878973 9 ACATGGG 9010 0.0 21.818819 3 GAGTACT 7575 0.0 21.769314 12-13 CAGAGTA 12265 0.0 21.279806 10-11 CATGGGG 6425 0.0 20.176472 4 TAACGCA 190 8.2796305E-6 19.99365 4 GTACTTT 8255 0.0 19.74614 14-15 >>END_MODULE