FastQCFastQC Report
Sat 18 Jun 2016
SRR1930966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440374
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45030.18452089720673964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.051.096931
GTATCAA29200.043.460111
TATCAAC38250.033.032132
ATCAACG39050.032.737643
TCAACGC39800.032.236574
CAACGCA40150.030.930125
AACGCAG44350.028.7864746
GTACATG73400.028.6096881
TACATGG76050.027.2042582
ACATGGG80500.025.2592353
ACGCAGA52950.023.5247447
CATGGGG68450.022.4926014
CGCAGAG58400.021.526818
TAGTACT5400.021.430184
GTACTAG4300.021.0803181
GAGTACA54050.019.1930771
GCAGAGT61250.019.0367819
AGTACAT52650.018.6436332
CTAGTAC5450.018.4700573
GTGGTAT8650.018.0475671
Produced by FastQC (version 0.11.5)