##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930966_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2440374 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.40402454705713 30.0 16.0 31.0 2.0 33.0 2 22.247851353931814 30.0 10.0 31.0 2.0 33.0 3 22.753379604929407 30.0 16.0 31.0 2.0 34.0 4 24.694938562695718 33.0 10.0 35.0 2.0 37.0 5 24.664280966769848 33.0 10.0 35.0 2.0 37.0 6 25.443351305988344 33.0 17.0 35.0 2.0 37.0 7 25.537397956214907 33.0 17.0 35.0 2.0 37.0 8 26.335291639724076 33.0 17.0 35.0 2.0 37.0 9 27.55788907765777 34.0 17.0 37.0 2.0 39.0 10-11 27.79863844640207 34.0 22.0 37.0 2.0 39.0 12-13 27.722437626363828 34.0 22.0 37.0 2.0 39.0 14-15 28.008945759953185 34.5 18.5 38.0 2.0 40.0 16-17 27.103674067991218 34.0 16.5 38.0 2.0 40.0 18-19 26.437436229037026 33.0 10.5 38.0 2.0 40.0 20-21 25.86431444524487 33.0 10.0 38.0 2.0 40.0 22-23 25.805173100516562 32.0 10.0 38.0 2.0 40.0 24-25 25.46559810094682 32.0 10.0 38.0 2.0 40.0 26-27 25.12452230682674 32.0 8.5 38.0 2.0 40.0 28-29 25.06432600085069 32.0 8.5 38.0 2.0 40.0 30-31 25.00142805979739 32.0 8.0 38.0 2.0 40.0 32-33 24.706759701586723 31.0 5.0 38.0 2.0 40.0 34-35 21.705437773062656 27.0 2.0 37.0 2.0 39.0 36-37 20.033620461453857 19.0 2.0 35.0 2.0 38.5 38-39 19.032786982651018 19.0 2.0 34.0 2.0 38.0 40-41 20.35772447174081 24.0 2.0 35.0 2.0 38.0 42-43 20.261330435416866 23.5 2.0 35.0 2.0 38.0 44-45 18.271974090856567 18.0 2.0 34.0 2.0 38.0 46-47 19.12134328590618 19.0 2.0 33.0 2.0 38.0 48-49 18.54158788775819 20.0 2.0 34.0 2.0 38.0 50-51 16.456806620624544 10.5 2.0 31.5 2.0 37.5 52-53 19.37916401338483 23.0 2.0 33.5 2.0 37.5 54-55 21.068828589388346 27.5 2.0 35.5 2.0 38.5 56-57 21.25732961423126 28.0 2.0 36.0 2.0 39.0 58-59 20.84966832952654 27.5 2.0 35.0 2.0 39.0 60-61 20.476180290398112 26.5 2.0 35.0 2.0 38.0 62-63 19.951651673063225 26.0 2.0 34.0 2.0 38.0 64-65 19.41754460586779 25.5 2.0 34.0 2.0 37.0 66-67 18.95782470227924 24.5 2.0 34.0 2.0 36.5 68-69 18.455401098356234 22.5 2.0 33.0 2.0 36.0 70-71 17.857760736673967 21.0 2.0 33.0 2.0 35.5 72-73 17.366334832283904 19.5 2.0 32.0 2.0 35.0 74-75 16.824547999609894 17.5 2.0 32.0 2.0 35.0 76-77 16.256640170727927 12.0 2.0 31.5 2.0 34.5 78-79 15.754259388110183 7.5 2.0 31.0 2.0 34.0 80-81 15.251870819800573 4.5 2.0 31.0 2.0 34.0 82-83 14.776904482673558 2.0 2.0 30.5 2.0 34.0 84-85 14.26320002589767 2.0 2.0 30.0 2.0 34.0 86-87 13.706795966519886 2.0 2.0 29.5 2.0 34.0 88-89 13.162463827265821 2.0 2.0 29.0 2.0 34.0 90-91 12.582546363795057 2.0 2.0 29.0 2.0 34.0 92-93 11.941582519728533 2.0 2.0 27.5 2.0 33.5 94-95 11.269900228407613 2.0 2.0 26.5 2.0 33.0 96-97 10.520178464448481 2.0 2.0 25.0 2.0 33.0 98-99 9.697772759421301 2.0 2.0 22.0 2.0 32.0 100-101 8.623961327239186 2.0 2.0 9.5 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.978589559323137 1101 2 8.142002084780017 1101 3 7.752385522498162 1101 4 9.496979324171537 1101 5 9.490069054828908 1101 6 8.866622534646574 1101 7 8.577878746213013 1101 8 7.41372838733454 1101 9 7.803600018203472 1101 10-11 7.198220728052476 1101 12-13 7.031515821443929 1101 14-15 7.7024690081199765 1101 16-17 8.365443491590515 1101 18-19 8.94158143609111 1101 20-21 9.411851745561673 1101 22-23 9.243074357189034 1101 24-25 9.232419559271136 1101 26-27 9.174554450038276 1101 28-29 9.026974634353707 1101 30-31 8.811082539674668 1101 32-33 8.802876091540867 1101 34-35 11.598671001729048 1101 36-37 5.356628348093917 1101 38-39 8.652713939861094 1101 40-41 10.075485377986649 1101 42-43 10.593779876867867 1101 44-45 4.861463335194447 1101 46-47 6.421897736078577 1101 48-49 9.718205113403851 1101 50-51 -3.8423628557779494 1101 52-53 6.118049505618391 1101 54-55 8.788767415325815 1101 56-57 9.382266131547357 1101 58-59 9.719837429456394 1101 60-61 9.315112048023849 1101 62-63 9.485892557683666 1101 64-65 9.629282251339774 1101 66-67 9.37064076347417 1101 68-69 9.15598838479767 1101 70-71 9.146883592044137 1101 72-73 9.183367369260512 1101 74-75 9.232452382261613 1101 76-77 9.142653181090177 1101 78-79 8.976445086113161 1101 80-81 8.751570780914586 1101 82-83 8.665525380119734 1101 84-85 8.538744789009506 1101 86-87 8.37188044263705 1101 88-89 8.205167743492618 1101 90-91 8.14004992016505 1101 92-93 7.979772329989492 1101 94-95 7.906032465747764 1101 96-97 7.679095587374107 1101 98-99 7.3466659228294535 1101 100-101 6.916579612332267 1102 1 6.6556924997613365 1102 2 7.787889946136957 1102 3 7.51797276413372 1102 4 9.299901850631638 1102 5 9.231417808785451 1102 6 8.64778462225047 1102 7 8.464667103210711 1102 8 7.554974846047362 1102 9 7.537077988313801 1102 10-11 6.985304845670001 1102 12-13 6.98807001499112 1102 14-15 7.324042613282025 1102 16-17 8.073343544705946 1102 18-19 8.38946121919172 1102 20-21 8.826908886488635 1102 22-23 8.740953613473788 1102 24-25 8.726681527442778 1102 26-27 8.872167958712456 1102 28-29 8.751403380029942 1102 30-31 8.362465369320393 1102 32-33 8.31186806348699 1102 34-35 10.86476600088304 1102 36-37 1.254509200320829 1102 38-39 3.5520062712195895 1102 40-41 8.895561028743682 1102 42-43 10.212882405769914 1102 44-45 1.3820619126676768 1102 46-47 2.0766267296061756 1102 48-49 8.310554383639932 1102 50-51 -4.962559379784947 1102 52-53 5.0174089285257075 1102 54-55 8.044333922109033 1102 56-57 8.639221185477808 1102 58-59 9.010301922997325 1102 60-61 8.746346774237644 1102 62-63 8.792579648278021 1102 64-65 8.667196413823419 1102 66-67 8.810743737380673 1102 68-69 8.616079294621048 1102 70-71 8.783406991655035 1102 72-73 8.711157990326825 1102 74-75 8.377576902936656 1102 76-77 8.414329329409297 1102 78-79 8.483281529002918 1102 80-81 8.288011253511792 1102 82-83 8.01640976449202 1102 84-85 7.724824981539008 1102 86-87 7.941179349897006 1102 88-89 7.796475157898987 1102 90-91 7.605901716328217 1102 92-93 7.542911365371067 1102 94-95 7.282431084374608 1102 96-97 7.045048609654696 1102 98-99 6.544972349301142 1102 100-101 5.866002346109902 1103 1 6.9148773574518145 1103 2 7.920910937502857 1103 3 7.522267718432669 1103 4 9.472684995546768 1103 5 9.3105227305022 1103 6 8.591384326092651 1103 7 8.373416199662987 1103 8 7.616069503946331 1103 9 7.780030618803043 1103 10-11 7.186469453599912 1103 12-13 7.065102982223667 1103 14-15 7.6168819749081536 1103 16-17 8.206581569142 1103 18-19 8.633763117338138 1103 20-21 9.068656635338083 1103 22-23 8.944015293243158 1103 24-25 8.909349514671426 1103 26-27 8.917498962900556 1103 28-29 8.857585598816229 1103 30-31 8.699248100752715 1103 32-33 8.509878956912559 1103 34-35 10.888763819655285 1103 36-37 -3.057577014174438 1103 38-39 -1.6370654780366856 1103 40-41 7.396874653940586 1103 42-43 9.92858157008665 1103 44-45 1.1701957884726966 1103 46-47 0.8098213474281941 1103 48-49 8.132458620294972 1103 50-51 -5.2640962584558935 1103 52-53 4.8914888838719826 1103 54-55 8.098001553972516 1103 56-57 8.896890444029882 1103 58-59 9.028691010925684 1103 60-61 9.064086291570625 1103 62-63 8.917709958928928 1103 64-65 8.811748838358263 1103 66-67 8.816861073267951 1103 68-69 8.833445784472861 1103 70-71 8.775078309751056 1103 72-73 8.60673186269322 1103 74-75 8.397547653417035 1103 76-77 8.423173805387941 1103 78-79 8.3197505916501 1103 80-81 8.028926739562953 1103 82-83 7.844660175250565 1103 84-85 7.792320677201133 1103 86-87 7.505885020362712 1103 88-89 7.5448265238451935 1103 90-91 7.3218737611369225 1103 92-93 7.275822508339255 1103 94-95 7.083717709083565 1103 96-97 6.879923674885699 1103 98-99 6.501003477352471 1103 100-101 5.804033123639201 1104 1 6.545667233210729 1104 2 7.705841177541455 1104 3 7.128164143988538 1104 4 9.074691348327118 1104 5 8.923561008014161 1104 6 8.244479296138088 1104 7 8.107448807921603 1104 8 7.153452399688366 1104 9 7.306497186291278 1104 10-11 6.636740351432728 1104 12-13 6.521312020863039 1104 14-15 6.951458389205932 1104 16-17 7.790564923249189 1104 18-19 8.329588576708492 1104 20-21 8.529137717723192 1104 22-23 8.214284884333185 1104 24-25 8.388140073957345 1104 26-27 8.70191153175585 1104 28-29 8.39038232994887 1104 30-31 7.919542318918456 1104 32-33 7.925769954459895 1104 34-35 10.281618449545519 1104 36-37 -3.375831708396621 1104 38-39 -2.076756785181555 1104 40-41 6.787181135384838 1104 42-43 8.761305927772305 1104 44-45 0.4518114251579952 1104 46-47 -0.3371671130277605 1104 48-49 6.784875089274909 1104 50-51 -5.738850992885551 1104 52-53 3.8982336870564147 1104 54-55 6.927051954036848 1104 56-57 7.542325890935917 1104 58-59 7.752154080363983 1104 60-61 7.644599238376145 1104 62-63 7.837475951450337 1104 64-65 8.005621722440125 1104 66-67 7.9429868824432965 1104 68-69 7.693699694649371 1104 70-71 7.627275648016504 1104 72-73 7.472637435448636 1104 74-75 7.381401841025092 1104 76-77 7.49977908864957 1104 78-79 7.0593871161163495 1104 80-81 6.9981881283289855 1104 82-83 7.02378948247585 1104 84-85 6.686386557141628 1104 86-87 6.379196306599695 1104 88-89 6.424430279222944 1104 90-91 6.051058856227586 1104 92-93 6.073071312167004 1104 94-95 5.863875824961497 1104 96-97 5.726723160230076 1104 98-99 5.5976270550132 1104 100-101 5.160836730249308 1105 1 6.163479083966468 1105 2 7.143809855041443 1105 3 6.8302551085481475 1105 4 8.881975181079302 1105 5 8.837310260727506 1105 6 7.886996289664097 1105 7 7.705038648873174 1105 8 6.899277793024549 1105 9 6.965263720879829 1105 10-11 6.496534480378969 1105 12-13 6.070111343616627 1105 14-15 6.4829697469359076 1105 16-17 7.049641612982807 1105 18-19 7.78566646415516 1105 20-21 8.120268463319373 1105 22-23 7.9566054042011025 1105 24-25 8.104972212463565 1105 26-27 7.962416026921712 1105 28-29 7.804856564444123 1105 30-31 7.52634099130886 1105 32-33 7.3186318664513195 1105 34-35 9.593170217250819 1105 36-37 -3.6651748650704583 1105 38-39 -2.5497185819213435 1105 40-41 5.846509972916426 1105 42-43 8.1859528724301 1105 44-45 -3.2222515573499955 1105 46-47 -4.589898432915753 1105 48-49 4.9919024746711465 1105 50-51 -10.019443595239244 1105 52-53 0.5009270886583188 1105 54-55 5.398357760611912 1105 56-57 6.576595422448445 1105 58-59 6.8658076862550566 1105 60-61 6.950104404155653 1105 62-63 6.931720941731573 1105 64-65 6.877268544127624 1105 66-67 6.69239213471289 1105 68-69 6.718931423215491 1105 70-71 6.712904573564298 1105 72-73 6.713790177759545 1105 74-75 6.541537337265986 1105 76-77 6.422422409939253 1105 78-79 6.334394328514806 1105 80-81 6.185866695722135 1105 82-83 6.1656557459721295 1105 84-85 5.897692589700284 1105 86-87 5.841083659941479 1105 88-89 5.670922544274111 1105 90-91 5.333072660596821 1105 92-93 5.10770483868207 1105 94-95 5.061228104328869 1105 96-97 4.759610009657795 1105 98-99 4.592704794836822 1105 100-101 4.055571224473081 1106 1 5.993630330210102 1106 2 7.190410317829297 1106 3 6.683452228029235 1106 4 8.093516554396619 1106 5 8.191327085922069 1106 6 7.414472908308262 1106 7 7.084285249959425 1106 8 6.32299142507792 1106 9 6.3144332678476935 1106 10-11 5.8684205041738835 1106 12-13 5.865032760528866 1106 14-15 6.126322426432452 1106 16-17 6.691165095874094 1106 18-19 7.0903464909462635 1106 20-21 7.541280940896964 1106 22-23 7.10822639377383 1106 24-25 7.2582436725135295 1106 26-27 7.160832088878518 1106 28-29 7.28885716178128 1106 30-31 6.889787252637337 1106 32-33 6.886642873249443 1106 34-35 9.325450946714685 1106 36-37 -0.24090917116478394 1106 38-39 0.6978803423967506 1106 40-41 6.666959314747071 1106 42-43 7.9441544570955145 1106 44-45 -3.529321452329098 1106 46-47 -4.93713591184418 1106 48-49 4.311066962680165 1106 50-51 -9.999965787264733 1106 52-53 -0.25067595407979226 1106 54-55 4.730806988437848 1106 56-57 5.897970087633841 1106 58-59 6.381474278304058 1106 60-61 6.347829490713131 1106 62-63 6.283240796660589 1106 64-65 6.287226573176262 1106 66-67 6.095577200571391 1106 68-69 5.840475729778895 1106 70-71 5.69558483905276 1106 72-73 5.444600795754116 1106 74-75 5.492555393355115 1106 76-77 5.29702836632889 1106 78-79 4.963632690979706 1106 80-81 4.888858384066522 1106 82-83 4.705032051162727 1106 84-85 4.576478985851203 1106 86-87 4.249012984148894 1106 88-89 4.197706450728502 1106 90-91 4.023881060843594 1106 92-93 3.9143781665091364 1106 94-95 3.5860226435034406 1106 96-97 3.2036199678501305 1106 98-99 2.8402370679659725 1106 100-101 2.580074665693264 1107 1 5.935462277688654 1107 2 7.131609636270806 1107 3 6.6405821126996365 1107 4 7.829102081640247 1107 5 8.322970482316833 1107 6 6.710981935792223 1107 7 6.917796024512658 1107 8 6.5357070384661675 1107 9 6.33528570525397 1107 10-11 5.857262862112975 1107 12-13 5.548347385542176 1107 14-15 5.955068263831251 1107 16-17 6.881657815459402 1107 18-19 7.09567325334196 1107 20-21 7.4240404324911715 1107 22-23 7.488178342173402 1107 24-25 7.392752320074678 1107 26-27 7.396256796525041 1107 28-29 7.339960952662551 1107 30-31 7.169280487420995 1107 32-33 6.905881430233549 1107 34-35 9.376405790586556 1107 36-37 -0.23628519400620362 1107 38-39 0.7099846254507547 1107 40-41 6.798354106350775 1107 42-43 7.9796584592369015 1107 44-45 -3.477468321275877 1107 46-47 -4.684886668092039 1107 48-49 4.546246087648864 1107 50-51 -9.968282887532693 1107 52-53 0.08849236035814201 1107 54-55 4.948350427717838 1107 56-57 6.089833263615812 1107 58-59 6.468571149985127 1107 60-61 6.541187533419908 1107 62-63 6.149122865167573 1107 64-65 6.31697920601253 1107 66-67 6.163420764477571 1107 68-69 6.050349261535548 1107 70-71 6.156815803337135 1107 72-73 5.878415724417852 1107 74-75 5.791251855180068 1107 76-77 5.7587507118341446 1107 78-79 5.719895301106954 1107 80-81 5.893387884208993 1107 82-83 5.717554393214138 1107 84-85 5.553314344000402 1107 86-87 5.43975207791787 1107 88-89 5.177738844904168 1107 90-91 5.050438384575239 1107 92-93 4.69968529955983 1107 94-95 4.582040073901295 1107 96-97 4.280838630694543 1107 98-99 4.059996939482987 1107 100-101 3.530145469489039 1108 1 6.161948148762683 1108 2 6.654007387968992 1108 3 6.904208886789508 1108 4 8.361673245677462 1108 5 8.250281757918557 1108 6 7.716836667662989 1108 7 7.747661041346337 1108 8 6.740340570429243 1108 9 5.033022274390579 1108 10-11 5.882544963056489 1108 12-13 5.379497325548886 1108 14-15 6.182688255646227 1108 16-17 6.143392552377829 1108 18-19 6.901545414989901 1108 20-21 7.684876978132433 1108 22-23 7.577262630484231 1108 24-25 7.248231300809202 1108 26-27 7.545953971576477 1108 28-29 7.6359345346978955 1108 30-31 7.520058029780582 1108 32-33 7.508676817896195 1108 34-35 9.875119751907793 1108 36-37 -3.4832448482552003 1108 38-39 -2.461984876622342 1108 40-41 5.802544991692823 1108 42-43 8.370581658758347 1108 44-45 -3.0624889616180013 1108 46-47 -4.625259958143751 1108 48-49 5.087975274926244 1108 50-51 -10.008568331507464 1108 52-53 0.5175403389571613 1108 54-55 5.44515059950572 1108 56-57 6.621252877134907 1108 58-59 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7.404649596144724 2301 92-93 7.242015795506051 2301 94-95 6.822473470147703 2301 96-97 6.3567395881737525 2301 98-99 6.276819851402459 2301 100-101 5.36575404789807 2302 1 6.727050338208969 2302 2 8.039373143802841 2302 3 7.3963768177221425 2302 4 9.02092806923196 2302 5 9.258093109372012 2302 6 8.357444164136204 2302 7 8.22317894114357 2302 8 7.338388632049476 2302 9 7.405322809848737 2302 10-11 6.93297207767975 2302 12-13 6.694896012804197 2302 14-15 7.02807388269467 2302 16-17 7.75536085988443 2302 18-19 8.119860036361128 2302 20-21 8.391192414642244 2302 22-23 8.322163701297331 2302 24-25 8.131758571161086 2302 26-27 8.33182784716669 2302 28-29 8.37832346580025 2302 30-31 8.177419661555884 2302 32-33 8.41225925460904 2302 34-35 10.864828922319976 2302 36-37 12.237555223231627 2302 38-39 12.632697199427078 2302 40-41 11.08168564276264 2302 42-43 10.744885388415458 2302 44-45 12.29350002870282 2302 46-47 11.557964554878044 2302 48-49 11.793867880419079 2302 50-51 12.962652985840819 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2313 74-75 -14.434503430174297 2313 76-77 -13.906258599152821 2313 78-79 -13.431962514645049 2313 80-81 -12.904612992119194 2313 82-83 -12.420030227226215 2313 84-85 -11.910096763459341 2313 86-87 -11.36600868448765 2313 88-89 -10.842547057166156 2313 90-91 -10.28891313256793 2313 92-93 -9.642098028296452 2313 94-95 -9.049989721256992 2313 96-97 -8.309064973205118 2313 98-99 -7.505255552575289 2313 100-101 -6.461893088951099 2314 1 -21.131949267935184 2314 2 -19.962989991289714 2314 3 -20.441354572589002 2314 4 -22.349296428601836 2314 5 -22.309059312841455 2314 6 -23.120302980408894 2314 7 -23.194308258541344 2314 8 -24.005447523917443 2314 9 -25.211455449308414 2314 10-11 -25.46620082503786 2314 12-13 -25.380330454308844 2314 14-15 -25.650145888868934 2314 16-17 -24.76787663519237 2314 18-19 -24.08179415820366 2314 20-21 -23.51841766806749 2314 22-23 -23.436402663413187 2314 24-25 -23.106130361489562 2314 26-27 -22.770968529359504 2314 28-29 -22.734713162793682 2314 30-31 -22.64692062989744 2314 32-33 -22.348867173118407 2314 34-35 -19.449664972914373 2314 36-37 -17.798482586929854 2314 38-39 -16.80211965760325 2314 40-41 -18.10834980426375 2314 42-43 -17.995760535176508 2314 44-45 -16.042459010288912 2314 46-47 -16.846525401797813 2314 48-49 -16.307943856485213 2314 50-51 -14.246681397774779 2314 52-53 -17.089161270926777 2314 54-55 -18.726882346956277 2314 56-57 -18.904580144205415 2314 58-59 -18.470534995266267 2314 60-61 -18.104063863228134 2314 62-63 -17.583838028687172 2314 64-65 -17.054156734397154 2314 66-67 -16.60380456140697 2314 68-69 -16.11817960252404 2314 70-71 -15.532197232044613 2314 72-73 -15.042068446904306 2314 74-75 -14.507444606644885 2314 76-77 -13.942925265819488 2314 78-79 -13.463531142096029 2314 80-81 -12.970299266628997 2314 82-83 -12.494736109579156 2314 84-85 -11.98225362620444 2314 86-87 -11.440322409977846 2314 88-89 -10.916860782656352 2314 90-91 -10.354403328646361 2314 92-93 -9.703666655747432 2314 94-95 -9.049989721256992 2314 96-97 -8.313574777126686 2314 98-99 -7.514863395712544 2314 100-101 -6.472089167382471 2315 1 -21.131949267935184 2315 2 -19.962989991289714 2315 3 -20.441354572589002 2315 4 -17.554925826568997 2315 5 -22.309059312841455 2315 6 -23.120302980408894 2315 7 -23.194308258541344 2315 8 -24.005447523917443 2315 9 -20.793550836329523 2315 10-11 -19.451540934498375 2315 12-13 -16.03122959426193 2315 14-15 -15.97813650653899 2315 16-17 -20.92463973136125 2315 18-19 -24.08179415820366 2315 20-21 -23.51841766806749 2315 22-23 -23.436402663413187 2315 24-25 -23.106130361489562 2315 26-27 -22.770968529359504 2315 28-29 -22.734713162793682 2315 30-31 -22.64692062989744 2315 32-33 -22.348867173118407 2315 34-35 -19.449664972914373 2315 36-37 -17.798482586929854 2315 38-39 -16.80211965760325 2315 40-41 -18.10834980426375 2315 42-43 -17.995760535176508 2315 44-45 -16.042459010288912 2315 46-47 -16.846525401797813 2315 48-49 -16.307943856485213 2315 50-51 -14.246681397774779 2315 52-53 -17.089161270926777 2315 54-55 -18.726882346956277 2315 56-57 -18.904580144205415 2315 58-59 -18.470534995266267 2315 60-61 -18.104063863228134 2315 62-63 -17.583838028687172 2315 64-65 -17.054156734397154 2315 66-67 -16.60380456140697 2315 68-69 -16.11817960252404 2315 70-71 -15.532197232044613 2315 72-73 -15.042068446904306 2315 74-75 -14.507444606644885 2315 76-77 -13.942925265819488 2315 78-79 -13.463531142096029 2315 80-81 -12.970299266628997 2315 82-83 -12.494736109579156 2315 84-85 -11.98225362620444 2315 86-87 -11.440322409977846 2315 88-89 -10.916860782656352 2315 90-91 -10.354403328646361 2315 92-93 -9.703666655747432 2315 94-95 -9.049989721256992 2315 96-97 -8.313574777126686 2315 98-99 -7.514863395712544 2315 100-101 -6.472089167382471 2316 1 -21.131949267935184 2316 2 -19.962989991289714 2316 3 -20.441354572589002 2316 4 -22.349296428601836 2316 5 -22.309059312841455 2316 6 -23.120302980408894 2316 7 -23.194308258541344 2316 8 -24.005447523917443 2316 9 -25.211455449308414 2316 10-11 -25.46620082503786 2316 12-13 -25.380330454308844 2316 14-15 -25.650145888868934 2316 16-17 -24.76787663519237 2316 18-19 -24.08179415820366 2316 20-21 -23.51841766806749 2316 22-23 -23.436402663413187 2316 24-25 -23.106130361489562 2316 26-27 -22.770968529359504 2316 28-29 -22.734713162793682 2316 30-31 -22.64692062989744 2316 32-33 -22.348867173118407 2316 34-35 -19.449664972914373 2316 36-37 -17.798482586929854 2316 38-39 -16.80211965760325 2316 40-41 -18.10834980426375 2316 42-43 -17.995760535176508 2316 44-45 -16.042459010288912 2316 46-47 -16.846525401797813 2316 48-49 -16.307943856485213 2316 50-51 -14.246681397774779 2316 52-53 -17.089161270926777 2316 54-55 -18.726882346956277 2316 56-57 -18.904580144205415 2316 58-59 -18.470534995266267 2316 60-61 -18.104063863228134 2316 62-63 -17.583838028687172 2316 64-65 -17.054156734397154 2316 66-67 -16.60380456140697 2316 68-69 -16.11817960252404 2316 70-71 -15.532197232044613 2316 72-73 -15.042068446904306 2316 74-75 -14.507444606644885 2316 76-77 -13.942925265819488 2316 78-79 -13.463531142096029 2316 80-81 -12.970299266628997 2316 82-83 -12.494736109579156 2316 84-85 -11.98225362620444 2316 86-87 -11.440322409977846 2316 88-89 -10.916860782656352 2316 90-91 -10.354403328646361 2316 92-93 -9.703666655747432 2316 94-95 -9.049989721256992 2316 96-97 -8.313574777126686 2316 98-99 -7.514863395712544 2316 100-101 -6.472089167382471 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 392918.0 3 22107.0 4 48725.0 5 29151.0 6 25116.0 7 31813.0 8 41231.0 9 50376.0 10 54165.0 11 50842.0 12 43993.0 13 38207.0 14 36394.0 15 38264.0 16 42265.0 17 46415.0 18 48923.0 19 51730.0 20 54843.0 21 58560.0 22 62444.0 23 66454.0 24 71312.0 25 75383.0 26 80738.0 27 85947.0 28 91419.0 29 97143.0 30 100391.0 31 99602.0 32 96851.0 33 93021.0 34 84748.0 35 69248.0 36 43811.0 37 14689.0 38 1131.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8991965682472 16.553019843888553 12.301421139601112 26.246362448263138 2 17.553370450035676 18.696193367950126 36.90730025467822 26.843135927335982 3 20.86634020587985 22.214028851647495 26.867132963906776 30.052497978565878 4 14.764413760648454 14.93914679421334 33.113228799507034 37.18321064563117 5 16.40567628418902 35.26483939181617 31.822787995302228 16.506696328692588 6 35.764641685400825 31.67184194779295 16.564918134243637 15.998598232562589 7 31.22775813562921 29.34647738943407 19.61078211916608 19.814982355770645 8 28.497545447304322 32.234921584272975 18.836822901891956 20.430710066530743 9 28.172645247222842 14.632098402331259 18.128351013840152 39.06690533660575 10-11 27.883216157932573 23.761089292915123 25.470330662552378 22.885363886599926 12-13 27.941147102076474 21.520454343624134 25.38799240777731 25.15040614652208 14-15 25.19576336095724 22.606934781829548 23.048771260518258 29.148530596694954 16-17 25.655941934283632 25.37198118158401 23.60298806679927 25.369088817333086 18-19 25.518698260825374 24.86424235101659 24.782687076101954 24.834372312056086 20-21 26.598977849223605 23.93802235123591 24.540966985859892 24.92203281368059 22-23 26.43106414976508 23.759650351261737 24.245759535607487 25.563525963365695 24-25 26.023680590537452 24.015625605455885 24.299148486844654 25.66154531716201 26-27 25.826909340804814 24.23695206805294 24.3298199948459 25.606318596296347 28-29 25.767938254214563 24.374642195338748 24.38457598264459 25.4728435678021 30-31 26.082561771123558 24.11390466131554 24.2180260476962 25.585507519864702 32-33 25.90583573801748 24.21529587017252 24.125898384000863 25.75297000780914 34-35 26.10571266832229 24.203482386076537 24.270171763773206 25.420633181827966 36-37 26.493118417067674 24.313329070902174 23.913934784803313 25.27961772722684 38-39 26.313952622961278 24.26249813936675 24.004508048567843 25.41904118910413 40-41 26.386602911014656 24.172409326489653 24.045778238557567 25.395209523938128 42-43 26.393675217945194 24.159443459146203 23.93720180850798 25.509679514400617 44-45 26.349095453641596 24.373306848723413 23.898395956321774 25.379201741313214 46-47 26.003600666581328 24.1089172281209 24.10043816330773 25.787043941990046 48-49 26.335605388979005 24.414465099258653 23.860288009331313 25.389641502431033 50-51 26.339224928672028 24.468561123785864 23.954921760098948 25.23729218744316 52-53 25.778630109422984 24.268038663203296 24.11835246887614 25.834978758497584 54-55 25.460676258323556 24.145797381846954 24.368119102801245 26.02540725702825 56-57 25.579314164537625 24.287383835243258 24.135919685690865 25.99738231452825 58-59 25.584017495640865 24.370765729822384 24.294068623104888 25.751148151431863 60-61 25.654420508620092 24.31037867530335 24.287201086599175 25.74799972947738 62-63 25.572572202100403 24.372147230538623 24.14971071283652 25.90556985452445 64-65 25.41896879527731 24.25065090028336 24.232640956323273 26.09773934811606 66-67 25.561510118093384 24.391534169490072 24.044565494126104 26.00239021829044 68-69 25.400823975250077 24.290379917932725 24.199631770579625 26.109164336237573 70-71 25.21652282886327 24.13380421312356 24.302978271336894 26.34669468667627 72-73 25.436334458524705 24.281191780569237 24.178656410823972 26.103817350082082 74-75 25.328659677720832 24.34786120258174 24.12914704246673 26.194332077230698 76-77 25.31577527957057 24.16027234809722 24.163781988525322 26.360170383806892 78-79 25.291077083170542 24.18320460594487 24.106526966670152 26.419191344214433 80-81 25.256900673111417 24.31488323097189 24.126924943520923 26.301291152395773 82-83 25.42030135717918 24.25033581689598 24.208965912019394 26.12039691390544 84-85 25.388748125207755 24.182980178635834 23.971504297070528 26.456767399085884 86-87 25.047844723255718 24.307583226092866 24.196050098427015 26.448521952224404 88-89 25.091316157861073 24.19697405137809 24.12892729008872 26.582782500672124 90-91 25.109846195041403 24.20727493532294 24.055133390944263 26.627745478691395 92-93 25.14084911317567 23.99895514477078 24.205505409523536 26.654690332530016 94-95 25.037083367472114 23.949796956013483 24.27653679313978 26.736582883374627 96-97 24.898833938906442 24.00349019467408 24.183578179534294 26.914097686885185 98-99 24.815541671422814 23.88136538816817 24.219521799662324 27.08357114074669 100-101 25.230525703934386 23.922673981132956 23.764542171580473 27.08225814335219 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27265.0 1 30412.5 2 22122.0 3 10593.0 4 13956.0 5 19462.5 6 21812.0 7 21586.0 8 20283.0 9 18616.0 10 16890.0 11 15313.0 12 13568.0 13 11641.0 14 9707.0 15 7862.0 16 6388.5 17 5155.5 18 4221.5 19 3719.0 20 3447.0 21 3521.0 22 3930.0 23 4388.0 24 4835.5 25 5343.0 26 6040.5 27 6663.5 28 7371.5 29 8162.0 30 8670.5 31 9389.0 32 10587.5 33 11965.0 34 13423.0 35 15216.5 36 17710.5 37 21235.5 38 25524.0 39 30512.0 40 36510.5 41 43433.0 42 50937.0 43 58895.0 44 67471.0 45 75721.0 46 83698.0 47 90977.5 48 97114.0 49 101858.5 50 104546.0 51 106422.0 52 107269.0 53 107041.0 54 105092.5 55 99419.5 56 91821.0 57 83398.5 58 74592.0 59 66049.0 60 57287.5 61 48092.0 62 39442.0 63 31917.5 64 25101.0 65 19501.0 66 14992.0 67 11548.0 68 8857.0 69 6784.5 70 5229.0 71 3973.0 72 3002.5 73 2251.5 74 1680.5 75 1229.0 76 842.0 77 547.5 78 352.5 79 229.5 80 143.0 81 92.0 82 51.5 83 24.0 84 15.5 85 13.0 86 10.5 87 8.5 88 4.5 89 2.0 90 2.0 91 1.5 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.371280795484626 2 21.320666422441807 3 17.089921462857742 4 22.328995473644614 5 22.158529799120956 6 16.043934249422424 7 14.894889062086387 8 12.02877099985494 9 3.0292897727971204 10-11 6.511051174942857 12-13 3.5614623004506685 14-15 7.39128510629928 16-17 11.453654234965624 18-19 14.53351002756135 20-21 14.96254262666296 22-23 12.862659575950245 24-25 15.399852645537118 26-27 12.942749758848437 28-29 12.961537862639085 30-31 13.792127764023054 32-33 12.290472689841803 34-35 19.10758350974072 36-37 23.892300934201067 38-39 22.918372347845043 40-41 18.06546865357523 42-43 17.977736199451396 44-45 23.34076662019838 46-47 12.768596125020181 48-49 21.447020005949906 50-51 21.151020294430282 52-53 11.2076058833605 54-55 12.781934244505145 56-57 12.290472689841803 58-59 13.340639590488998 60-61 12.750586590416061 62-63 12.697992192999926 64-65 13.767602834647477 66-67 12.12109291444672 68-69 13.14435820083315 70-71 11.74717481828605 72-73 11.139849875469908 74-75 11.212174855165642 76-77 11.265076582523827 78-79 11.635245253391489 80-81 11.639383963277758 82-83 10.160696680099033 84-85 10.072124190800261 86-87 10.188950546104818 88-89 11.597976375752243 90-91 9.098810264328337 92-93 11.131592944360168 94-95 11.226619362441987 96-97 10.747205141507 98-99 10.612594626889157 100-101 10.715386248173436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2440374.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.68047954318452 #Duplication Level Percentage of deduplicated Percentage of total 1 99.0911151203884 96.7926764342844 2 0.6454791493087513 1.2610142567921125 3 0.11309933654082667 0.3314279228797188 4 0.04221064341121973 0.1649262356093722 5 0.02894146494494883 0.14135080872524328 6 0.014842012304393084 0.08698649275673768 7 0.010679869021405419 0.07302503092284975 8 0.009533308623148279 0.07449745263538399 9 0.006914915620915688 0.06079070464665317 >10 0.03476419185771681 0.6029362831061522 >50 0.0017455309897315544 0.1204209852809977 >100 6.324829038343685E-4 0.10532219822376447 >500 0.0 0.0 >1k 4.197408474325587E-5 0.18462519413662062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4503 0.18452089720673964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2293197681994644E-4 2 0.0 0.0 0.0 0.0 1.2293197681994644E-4 3 0.0 0.0 0.0 0.0 1.2293197681994644E-4 4 0.0 0.0 0.0 0.0 1.6390930242659527E-4 5 0.0 0.0 0.0 0.0 2.458639536398929E-4 6 0.0 0.0 0.0 0.0 2.868412792465417E-4 7 0.0 0.0 0.0 4.097732560664882E-5 3.2781860485319055E-4 8 0.0 0.0 0.0 4.097732560664882E-5 3.2781860485319055E-4 9 0.0 0.0 0.0 4.097732560664882E-5 3.687959304598393E-4 10-11 0.0 0.0 0.0 4.097732560664882E-5 4.0977325606648816E-4 12-13 0.0 0.0 0.0 4.097732560664882E-5 5.941712212964078E-4 14-15 0.0 0.0 0.0 4.097732560664882E-5 7.580805237230031E-4 16-17 2.048866280332441E-5 0.0 0.0 4.097732560664882E-5 8.605238377396251E-4 18-19 4.097732560664882E-5 0.0 0.0 4.097732560664882E-5 8.605238377396251E-4 20-21 4.097732560664882E-5 0.0 0.0 4.097732560664882E-5 9.629671517562472E-4 22-23 4.097732560664882E-5 0.0 0.0 4.097732560664882E-5 0.0010244331401662204 24-25 4.097732560664882E-5 0.0 0.0 1.2293197681994644E-4 0.0010654104657728692 26-27 4.097732560664882E-5 0.0 0.0 3.0732994204986613E-4 0.0011473651169861669 28-29 4.097732560664882E-5 0.0 0.0 0.0010654104657728692 0.0011883424425928157 30-31 8.195465121329764E-5 0.0 0.0 0.003954311921041611 0.0011883424425928157 32-33 8.195465121329764E-5 0.0 0.0 0.012661993612454483 0.0011883424425928157 34-35 8.195465121329764E-5 0.0 0.0 0.024279065421939423 0.0012293197681994645 36-37 8.195465121329764E-5 0.0 0.0 0.039379209907989515 0.0012293197681994645 38-39 8.195465121329764E-5 0.0 0.0 0.0633714340506824 0.0012702970938061134 40-41 1.2293197681994644E-4 0.0 0.0 0.08918714918287116 0.0012702970938061134 42-43 1.2293197681994644E-4 0.0 0.0 0.11873180094526495 0.0012702970938061134 44-45 1.2293197681994644E-4 0.0 0.0 0.15649240649179183 0.0013727404078227353 46-47 1.2293197681994644E-4 0.0 0.0 0.20148551000789222 0.0014956723846426818 48-49 1.2293197681994644E-4 0.0 0.0 0.24529027108139984 0.0016800703498726015 50-51 1.2293197681994644E-4 0.0 0.0 0.29647095076410424 0.0017620250010858991 52-53 1.2293197681994644E-4 0.0 0.0 0.36611191563260387 0.0018849569779058456 54-55 1.2293197681994644E-4 0.0 0.0 0.4503612970798738 0.0018849569779058456 56-57 1.2293197681994644E-4 0.0 0.0 0.5453672264988891 0.0019054456407091701 58-59 1.2293197681994644E-4 0.0 0.0 0.6435898759780263 0.0019259343035124945 60-61 1.2293197681994644E-4 0.0 0.0 0.7461766106342717 0.001966911629119143 62-63 1.2293197681994644E-4 0.0 0.0 0.8516522467457857 0.00208984360593909 64-65 1.2293197681994644E-4 4.097732560664882E-5 0.0 0.9751579061242251 0.0021308209315457384 66-67 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.1202995934229754 0.002171798257152387 68-69 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.268494091479421 0.002212775582759036 70-71 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.4236137575633898 0.002212775582759036 72-73 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.5865191155126221 0.0022947302339723337 74-75 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.7616766938182424 0.0023766848851856314 76-77 1.2293197681994644E-4 4.097732560664882E-5 0.0 1.9676697096428661 0.0024996168620055777 78-79 1.2293197681994644E-4 4.097732560664882E-5 0.0 2.1865296057079773 0.0026020601760222 80-81 1.2293197681994644E-4 4.097732560664882E-5 0.0 2.410429712822707 0.002704503490038822 82-83 1.2293197681994644E-4 4.097732560664882E-5 0.0 2.641173033313746 0.0029503674436787146 84-85 1.2293197681994644E-4 4.097732560664882E-5 0.0 2.889618558466858 0.0029503674436787146 86-87 1.2293197681994644E-4 4.097732560664882E-5 0.0 3.1628758542747955 0.003011833432088688 88-89 1.2293197681994644E-4 4.097732560664882E-5 0.0 3.452667500965016 0.0030937880833019856 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 51.09693 1 GTATCAA 2920 0.0 43.46011 1 TATCAAC 3825 0.0 33.03213 2 ATCAACG 3905 0.0 32.73764 3 TCAACGC 3980 0.0 32.23657 4 CAACGCA 4015 0.0 30.93012 5 AACGCAG 4435 0.0 28.786474 6 GTACATG 7340 0.0 28.609688 1 TACATGG 7605 0.0 27.204258 2 ACATGGG 8050 0.0 25.259235 3 ACGCAGA 5295 0.0 23.524744 7 CATGGGG 6845 0.0 22.492601 4 CGCAGAG 5840 0.0 21.52681 8 TAGTACT 540 0.0 21.43018 4 GTACTAG 430 0.0 21.080318 1 GAGTACA 5405 0.0 19.193077 1 GCAGAGT 6125 0.0 19.036781 9 AGTACAT 5265 0.0 18.643633 2 CTAGTAC 545 0.0 18.470057 3 GTGGTAT 865 0.0 18.047567 1 >>END_MODULE