FastQCFastQC Report
Sat 18 Jun 2016
SRR1930966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440374
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.049.2747541
GTATCAA41150.041.5487021
TATCAAC53350.031.5029472
CAACGCA53550.031.1932645
TCAACGC54300.030.6888584
ATCAACG55100.030.2432843
AACGCAG57700.029.3609456
GTACATG103500.024.0903851
GTGGTAT11300.023.1158051
TACATGG104900.023.0369322
ACGCAGA72750.022.8303577
TATAGCG1252.2715956E-422.7781725
TATACCG2154.4718035E-822.0718735
ACATGGG110800.021.5955473
CTAGTAC6200.021.4407393
CGCAGAG77700.021.4369858
TAGATCG1605.0291965E-520.7613565
GTACTAG5200.020.0929681
CATGGGG88150.019.442784
GCAGAGT85350.019.126379
Produced by FastQC (version 0.11.5)