##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930966_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2440374 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.263045336493505 31.0 31.0 33.0 30.0 34.0 2 31.37437253470165 31.0 31.0 34.0 30.0 34.0 3 31.462797505628235 31.0 31.0 34.0 30.0 34.0 4 35.32342993328072 37.0 35.0 37.0 33.0 37.0 5 35.10086117947495 35.0 35.0 37.0 32.0 37.0 6 35.144256986838904 37.0 35.0 37.0 32.0 37.0 7 35.009634998569894 36.0 35.0 37.0 32.0 37.0 8 34.986221374264765 36.0 35.0 37.0 32.0 37.0 9 36.50021881891874 38.0 35.0 39.0 32.0 39.0 10-11 36.41820167728389 38.0 35.0 39.0 32.0 39.0 12-13 36.3007969270284 38.0 35.0 39.0 32.0 39.0 14-15 37.327586878076886 39.0 36.0 41.0 32.0 41.0 16-17 37.266619378832914 39.0 36.0 40.0 32.0 41.0 18-19 37.199388085596716 39.0 36.0 40.0 31.0 41.0 20-21 37.129601036562434 39.0 36.0 40.0 31.0 41.0 22-23 36.978819230167176 39.0 36.0 40.0 31.0 41.0 24-25 36.838790078897745 38.5 35.5 40.0 30.5 41.0 26-27 36.552593168096365 38.0 35.0 40.0 30.0 41.0 28-29 36.34312445551379 38.0 35.0 40.0 30.0 41.0 30-31 36.10085872083541 38.0 34.5 40.0 29.5 41.0 32-33 35.88985110478968 38.0 34.0 40.0 29.0 41.0 34-35 36.17108566146008 38.0 35.0 40.0 30.0 41.0 36-37 36.262840040092215 38.0 35.0 40.0 30.0 41.0 38-39 36.22667324762516 38.0 35.0 40.0 30.0 41.0 40-41 36.12311453080552 38.0 35.0 40.0 29.5 41.0 42-43 35.97753807408209 38.0 34.5 40.0 29.0 41.0 44-45 35.797132529686024 38.0 34.0 40.0 28.5 41.0 46-47 35.58728108888228 38.0 34.0 40.0 28.0 41.0 48-49 35.377124776776014 38.0 34.0 40.0 27.5 41.0 50-51 35.05231780046829 38.0 33.0 40.0 26.5 41.0 52-53 34.77886381349744 37.0 33.0 40.0 26.0 41.0 54-55 34.537446104572496 37.0 33.0 40.0 26.0 41.0 56-57 34.209392904530205 36.5 32.5 39.5 26.0 41.0 58-59 33.892166938346335 36.0 32.0 39.0 26.0 41.0 60-61 33.503548841284164 35.5 32.0 39.0 25.0 40.0 62-63 33.121500433949876 35.0 31.0 38.5 24.5 40.0 64-65 32.74558571759903 35.0 31.0 38.0 23.5 40.0 66-67 32.34904342531104 35.0 31.0 37.5 23.0 40.0 68-69 31.918905462851185 34.5 30.5 37.0 22.0 39.0 70-71 31.494685650642072 34.0 30.0 36.0 21.5 39.0 72-73 31.054114451309513 34.0 30.0 36.0 20.5 38.5 74-75 30.53956155900694 34.0 29.0 35.0 20.0 37.0 76-77 29.228701830129317 32.5 28.0 34.5 18.0 36.0 78-79 30.013923070807998 33.5 29.0 35.0 19.0 36.0 80-81 30.059350738862157 34.0 29.0 35.0 19.0 36.0 82-83 29.852293132118273 34.0 29.0 35.0 18.0 35.5 84-85 29.598908609909792 34.0 29.0 35.0 18.0 35.0 86-87 29.306864234744346 33.0 29.0 35.0 16.0 35.0 88-89 29.010389800907564 33.0 29.0 35.0 10.5 35.0 90-91 28.709341273099945 33.0 29.0 35.0 7.0 35.0 92-93 28.35964323501234 33.0 28.0 35.0 2.0 35.0 94-95 27.933109842999475 33.0 27.0 35.0 2.0 35.0 96-97 27.438988245244374 33.0 27.0 35.0 2.0 35.0 98-99 26.91661831342245 33.0 25.5 35.0 2.0 35.0 100-101 25.634769711527824 32.0 22.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4824327262480921 1101 2 0.549719186614265 1101 3 0.5002067747016135 1101 4 0.4327980980567432 1101 5 0.5159927957339576 1101 6 0.45661413502144654 1101 7 0.36133705358064105 1101 8 0.4597792230428368 1101 9 0.6708497723150657 1101 10-11 0.5339632444171727 1101 12-13 0.5846303586772308 1101 14-15 0.6944272870076347 1101 16-17 0.788174176546363 1101 18-19 0.6899861710215589 1101 20-21 0.7348106955107738 1101 22-23 0.743805915902847 1101 24-25 0.7002969151741212 1101 26-27 0.7297998661143694 1101 28-29 0.660094155830329 1101 30-31 0.7756836155128113 1101 32-33 0.6982139029255947 1101 34-35 0.7206169330497545 1101 36-37 0.7265959550468963 1101 38-39 0.761984580053344 1101 40-41 0.7079885414923268 1101 42-43 0.8608760855610171 1101 44-45 0.8838516720776681 1101 46-47 0.656248463981818 1101 48-49 0.7618877133590658 1101 50-51 0.7405225298102991 1101 52-53 0.6530928596060903 1101 54-55 0.5974383568658865 1101 56-57 0.7212865651034974 1101 58-59 0.6857477297058097 1101 60-61 0.5584533211134115 1101 62-63 0.45013037873489026 1101 64-65 0.5551058439568877 1101 66-67 0.6779245101965259 1101 68-69 0.6046489438567981 1101 70-71 0.5293395109501411 1101 72-73 0.5435849347887398 1101 74-75 0.5907365992733311 1101 76-77 0.6103409259676518 1101 78-79 0.5579826797655763 1101 80-81 0.5797699861928685 1101 82-83 0.7427984628047177 1101 84-85 0.8654404869106429 1101 86-87 0.7277125468297783 1101 88-89 0.7549330550939786 1101 90-91 0.6292479592827895 1101 92-93 0.7020939725889477 1101 94-95 0.7742254436079179 1101 96-97 0.7378967938314709 1101 98-99 0.900129216308283 1101 100-101 1.0728940026808225 1102 1 0.4084291984412012 1102 2 0.5122291108953334 1102 3 0.5235696634374847 1102 4 0.3014663137686213 1102 5 0.45235715893444706 1102 6 0.437334534013182 1102 7 0.40875435433734 1102 8 0.5608590715925743 1102 9 0.6790033323197022 1102 10-11 0.7247762576365275 1102 12-13 0.6809240851680727 1102 14-15 0.8688478825326769 1102 16-17 0.7138861681768063 1102 18-19 0.8164802362859831 1102 20-21 0.7966022443927798 1102 22-23 0.8276915020350728 1102 24-25 0.7713462455911682 1102 26-27 0.7741731008938828 1102 28-29 0.8824442255869442 1102 30-31 0.8551595205049836 1102 32-33 0.9316444596088118 1102 34-35 0.7755146316081962 1102 36-37 0.67874713012516 1102 38-39 0.7500975225554143 1102 40-41 0.7650182173394384 1102 42-43 0.7483979561663716 1102 44-45 0.9320989402275615 1102 46-47 0.8694841545785224 1102 48-49 0.6949214382508657 1102 50-51 0.8180828393796986 1102 52-53 0.8962804333215715 1102 54-55 0.8577053006583526 1102 56-57 0.6816071821350178 1102 58-59 0.7845511139708776 1102 60-61 0.8607899973515956 1102 62-63 0.7357807824927605 1102 64-65 0.7329023219759634 1102 66-67 0.9082098987418163 1102 68-69 0.7766079922910087 1102 70-71 0.7944581963648396 1102 72-73 0.6900122400884428 1102 74-75 0.8410615981732157 1102 76-77 1.0395169642752187 1102 78-79 1.050177515482698 1102 80-81 0.954762316373774 1102 82-83 1.161464447296499 1102 84-85 1.1505978530048964 1102 86-87 1.223702006174161 1102 88-89 1.1703996404037476 1102 90-91 1.1100188706184433 1102 92-93 1.1227026680255463 1102 94-95 1.221860722172643 1102 96-97 1.1402520945596564 1102 98-99 1.2431596284580202 1102 100-101 1.533221592275158 1103 1 0.4932496270345972 1103 2 0.5356732229122443 1103 3 0.47395354257216127 1103 4 0.2841452888640035 1103 5 0.35467396425608655 1103 6 0.3884689480117913 1103 7 0.4962554591779309 1103 8 0.4398743347383629 1103 9 0.6246554667506459 1103 10-11 0.5796175329071147 1103 12-13 0.6727411514137174 1103 14-15 0.6880372382865048 1103 16-17 0.6938493542828184 1103 18-19 0.7155598586666017 1103 20-21 0.6889874408606786 1103 22-23 0.7808312773861701 1103 24-25 0.791204695835809 1103 26-27 0.9556036666570265 1103 28-29 0.8828102607892916 1103 30-31 0.9796625692596308 1103 32-33 0.849006847929104 1103 34-35 0.7513644810471831 1103 36-37 0.7713296692849454 1103 38-39 0.800300646741924 1103 40-41 0.9558247933506507 1103 42-43 0.9164582859113395 1103 44-45 0.8498615684042008 1103 46-47 0.7560188417300466 1103 48-49 0.87497329916566 1103 50-51 0.8517114628027969 1103 52-53 0.7218415409954062 1103 54-55 0.9529495975620179 1103 56-57 0.9527211399343471 1103 58-59 0.9572367907568733 1103 60-61 0.8072570652317879 1103 62-63 0.6498968313164255 1103 64-65 0.7170468756869539 1103 66-67 0.5859351621176572 1103 68-69 0.5721231113534735 1103 70-71 0.5629438443341748 1103 72-73 0.6775665134907811 1103 74-75 0.857680746604462 1103 76-77 1.011746871613905 1103 78-79 1.0099367994733441 1103 80-81 1.1035564963762212 1103 82-83 0.8710906963717626 1103 84-85 1.00088756339742 1103 86-87 1.0949867148171002 1103 88-89 0.8964494205858742 1103 90-91 1.0410522234857602 1103 92-93 1.0833889586791372 1103 94-95 1.1967405064351162 1103 96-97 1.2276748011224683 1103 98-99 1.2565736040558768 1103 100-101 1.35954095356227 1104 1 0.43888410091984653 1104 2 0.4958376714667878 1104 3 0.43754567799092214 1104 4 0.3347514164825114 1104 5 0.4085972081948128 1104 6 0.3836691812151187 1104 7 0.3328874804074786 1104 8 0.3622186313086644 1104 9 0.38946186777995706 1104 10-11 0.5337483479125424 1104 12-13 0.5750922832540084 1104 14-15 0.7093170420740478 1104 16-17 0.6524909448903529 1104 18-19 0.735311778573319 1104 20-21 0.5536591468636516 1104 22-23 0.5997921153098531 1104 24-25 0.6059764179217737 1104 26-27 0.7957588674933405 1104 28-29 0.6463677172647024 1104 30-31 0.7825379059971951 1104 32-33 0.821004857659311 1104 34-35 0.6718994414428252 1104 36-37 0.7700555950619545 1104 38-39 0.7051270509878336 1104 40-41 0.7809222964667271 1104 42-43 0.819513048486229 1104 44-45 0.9120725772096421 1104 46-47 0.8878490857893908 1104 48-49 0.9310501557456163 1104 50-51 0.8609792847641131 1104 52-53 0.9506723077518657 1104 54-55 0.9238469277859664 1104 56-57 0.9723021780912333 1104 58-59 0.8710520776371737 1104 60-61 0.8703099179518219 1104 62-63 1.0496495553725609 1104 64-65 0.9729046015524219 1104 66-67 1.0544024533403658 1104 68-69 0.8969115195397173 1104 70-71 0.8399733164100915 1104 72-73 0.7462433861537257 1104 74-75 0.7547018756302357 1104 76-77 0.8839524524108171 1104 78-79 0.9905065393310117 1104 80-81 0.9644889076182324 1104 82-83 1.014174006250741 1104 84-85 0.8848035699914476 1104 86-87 1.0681532541601122 1104 88-89 1.2825416565233105 1104 90-91 1.1844848357651223 1104 92-93 1.0010691886658734 1104 94-95 1.1400404340616674 1104 96-97 1.1801244412656047 1104 98-99 1.257417960937385 1104 100-101 1.6775820455971697 1105 1 0.21312883880382216 1105 2 0.3731966687100794 1105 3 0.4207457054611332 1105 4 0.24209256689162117 1105 5 0.27311931012397395 1105 6 0.27789800046257795 1105 7 0.3224825500911308 1105 8 0.3326931410930172 1105 9 0.36876294454776115 1105 10-11 0.3207745124103951 1105 12-13 0.3268930349869663 1105 14-15 0.5152049859080279 1105 16-17 0.4092710396684609 1105 18-19 0.3172016294740416 1105 20-21 0.3437127578026704 1105 22-23 0.5578391416152257 1105 24-25 0.6104842036435372 1105 26-27 0.3871419369786011 1105 28-29 0.5249864262996695 1105 30-31 0.595237641009426 1105 32-33 0.6411372517617835 1105 34-35 0.6285960690338968 1105 36-37 0.6373736569914357 1105 38-39 0.5307686022683527 1105 40-41 0.5331281311283291 1105 42-43 0.4766292424998966 1105 44-45 0.7043337837213741 1105 46-47 0.49071975244392974 1105 48-49 0.5732837893240728 1105 50-51 0.6658117091249167 1105 52-53 0.5275747447648129 1105 54-55 0.6810447253557399 1105 56-57 0.7511663728104381 1105 58-59 0.7281007260351728 1105 60-61 0.6910266410605814 1105 62-63 0.5717178071135791 1105 64-65 0.5207448147165863 1105 66-67 0.5223038317522324 1105 68-69 0.6546989288491183 1105 70-71 0.5628703996662558 1105 72-73 0.48596208647218475 1105 74-75 0.658087799552419 1105 76-77 0.651690036740117 1105 78-79 0.7617898649407913 1105 80-81 0.6673912119758683 1105 82-83 0.7397575061613715 1105 84-85 0.8058721965130573 1105 86-87 0.7879851781286646 1105 88-89 0.7556955393176565 1105 90-91 0.7024172208869501 1105 92-93 0.731637020869826 1105 94-95 0.6435623296707504 1105 96-97 0.6534194170633505 1105 98-99 0.9169824083766827 1105 100-101 1.3271292566514639 1106 1 0.02919248786883699 1106 2 0.09457451058911559 1106 3 0.09965516137961217 1106 4 -0.08811642392328167 1106 5 0.06806477490394514 1106 6 -0.00529711206065997 1106 7 0.08243614067529137 1106 8 -0.045319893445061155 1106 9 0.027113106440381785 1106 10-11 0.017232974023769998 1106 12-13 -0.03778456623525983 1106 14-15 -0.04495164587042666 1106 16-17 -0.10802019366651194 1106 18-19 -0.025770692914640847 1106 20-21 -0.02013563731389212 1106 22-23 0.09963478105396462 1106 24-25 0.036025696309529565 1106 26-27 0.18456885966499925 1106 28-29 0.16263085452747106 1106 30-31 0.14398103601019585 1106 32-33 0.09712898582680651 1106 34-35 -0.09198670877390214 1106 36-37 0.03290637960789411 1106 38-39 -0.006507448252357051 1106 40-41 -0.03187384910967239 1106 42-43 -0.02498196463775315 1106 44-45 -0.11149317197889275 1106 46-47 -0.1594674943483554 1106 48-49 -0.12479671581177598 1106 50-51 -0.07045131754566114 1106 52-53 -0.22075192443924863 1106 54-55 -0.08408516875645233 1106 56-57 0.047946303944478075 1106 58-59 0.11150619802356232 1106 60-61 -0.06703503548549605 1106 62-63 -0.20792876048378872 1106 64-65 -0.2411386172525951 1106 66-67 -0.242006133981036 1106 68-69 -0.20063822819414057 1106 70-71 -0.1192993978744461 1106 72-73 -0.12470887278835363 1106 74-75 0.039681442840002745 1106 76-77 -0.02395254603650443 1106 78-79 0.004726397570781415 1106 80-81 -0.03925552959743328 1106 82-83 0.07247682716834092 1106 84-85 -0.03305451746160415 1106 86-87 -0.012045742679035243 1106 88-89 0.03514564177344681 1106 90-91 -0.030408519356885932 1106 92-93 0.04718052346253998 1106 94-95 -0.017094423534594227 1106 96-97 0.0048824180828646035 1106 98-99 -0.12021244347954863 1106 100-101 0.2633791015278746 1107 1 0.15461205792285782 1107 2 0.17143577256603848 1107 3 0.21882269870270932 1107 4 0.060332944167228675 1107 5 0.12709456142761866 1107 6 0.1850496826284811 1107 7 0.24649717009340577 1107 8 0.12647126989583768 1107 9 0.19668503129625492 1107 10-11 0.1450667808215087 1107 12-13 0.16492957926941898 1107 14-15 0.16424781072483086 1107 16-17 0.19928907501086002 1107 18-19 0.23679726865559303 1107 20-21 0.20125344105669996 1107 22-23 0.3068471262426229 1107 24-25 0.25836220717906855 1107 26-27 0.24817851488504772 1107 28-29 0.24042156023408978 1107 30-31 0.27457647866344104 1107 32-33 0.2833782986558262 1107 34-35 0.2972787450179837 1107 36-37 0.35215026126769544 1107 38-39 0.29751160257382736 1107 40-41 0.10716811221303857 1107 42-43 0.2600165530876595 1107 44-45 0.3374693077657511 1107 46-47 0.2939012984469329 1107 48-49 0.18862463297175225 1107 50-51 0.14265328498888863 1107 52-53 -0.01569297884017118 1107 54-55 0.28847627368379847 1107 56-57 0.307070783349495 1107 58-59 0.3067406852285046 1107 60-61 0.22402022889184536 1107 62-63 0.05526780063917869 1107 64-65 0.042727543425968406 1107 66-67 0.10540261978581356 1107 68-69 0.19814350039706952 1107 70-71 0.0377393197133884 1107 72-73 0.21391114396278965 1107 74-75 0.39830439535829143 1107 76-77 0.3471379907195882 1107 78-79 0.5657620757761634 1107 80-81 0.6554792295977094 1107 82-83 0.5978986997848317 1107 84-85 0.5479497019841091 1107 86-87 0.5892604578338592 1107 88-89 0.5965451248481735 1107 90-91 0.5523203193745125 1107 92-93 0.5762706659048114 1107 94-95 0.7519653970382265 1107 96-97 0.7603325834356305 1107 98-99 0.9957866959377348 1107 100-101 1.1280182180346578 1108 1 0.31660705627400176 1108 2 0.2749074355829073 1108 3 0.29981218018078337 1108 4 0.2622472914534484 1108 5 0.31800430516109657 1108 6 0.24771726122109072 1108 7 0.272472000100791 1108 8 0.3056182844887445 1108 9 0.25791569905890555 1108 10-11 0.4205237721491457 1108 12-13 0.36628958992250205 1108 14-15 0.42335685629115005 1108 16-17 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1251.0 9 1931.0 10 2898.0 11 4057.0 12 5419.0 13 6968.0 14 8309.0 15 9837.0 16 11442.0 17 12901.0 18 13580.0 19 13643.0 20 14810.0 21 15958.0 22 17854.0 23 21432.0 24 25953.0 25 32909.0 26 42076.0 27 54204.0 28 67156.0 29 82573.0 30 100127.0 31 120166.0 32 143306.0 33 173373.0 34 213558.0 35 266270.0 36 344028.0 37 392477.0 38 207713.0 39 11260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43415299516204 17.514021250329936 12.92963389822728 26.12219185628075 2 17.930880742727133 19.50404539762259 35.94761144513143 26.617462414518844 3 20.83961160433472 22.63816464812656 27.57732159431544 28.94490215322328 4 15.366469022141244 15.679598616966551 32.971984759578035 35.98194760131417 5 16.14936535092662 35.74540276397611 32.103797649903186 16.001434235194083 6 35.805536364508065 32.455394132210884 16.179774083808464 15.559295419472589 7 31.173910228514156 29.588087727536845 19.49725738759715 19.740744656351854 8 28.42228281402769 32.69814380910467 18.663696630106696 20.215876746760948 9 27.87646483694712 14.328254603597646 17.96765577735216 39.82762478210307 10-11 27.692189803694024 23.824032709740393 25.702371849560762 22.78140563700482 12-13 27.87527649450453 21.151286647046724 25.75734293186208 25.216093926586662 14-15 25.08726121487936 22.483643900484108 23.12739768576456 29.301697198871977 16-17 25.59460148321528 25.12854587042806 23.849008389697644 25.42784425665902 18-19 25.563233340463388 24.59854514103166 24.96041590346398 24.877805615040973 20-21 26.491144390163146 23.742795161725212 24.633683197739362 25.13237725037228 22-23 26.308308953526993 23.552993015208532 24.489222653827376 25.649475377437103 24-25 25.945398618523214 23.807578350648747 24.56232370681451 25.684699324013533 26-27 25.835338528263414 24.069284948580684 24.470911287335145 25.624465235820757 28-29 25.75456702725362 24.185462632146805 24.55446360044141 25.50550674015816 30-31 26.052423550355076 23.957841997442014 24.418685182081312 25.5710492701216 32-33 25.966086578555352 23.99342905173126 24.300130685307852 25.740353684405537 34-35 25.97197177095458 24.06780216717759 24.509331814232553 25.450894247635286 36-37 25.930558663018026 23.97068549665992 24.45827709167738 25.640478748644668 38-39 25.882696911359872 23.962162171022168 24.567994420499907 25.587146497118052 40-41 25.978031191356393 23.861836350795294 24.534001332200422 25.626131125647888 42-43 25.908616835336957 23.91000198757051 24.399784440806258 25.78159673628628 44-45 25.744384307493206 23.880088731486442 24.504717087450018 25.870809873570337 46-47 25.845246408455786 23.910304010858233 24.43303269928071 25.811416881405275 48-49 25.85909216461063 24.040278115873964 24.403393420197418 25.69723629931799 50-51 25.75080328659324 23.993333537238364 24.514687217556467 25.741175958611933 52-53 25.925733845036824 23.918824126046 24.40165170736147 25.75379032155571 54-55 25.694043990444023 23.834304874015295 24.46571410186894 26.005937033671746 56-57 25.670507269852216 23.97828951668807 24.401323464112192 25.94987974934752 58-59 25.6815418560792 24.047513607680347 24.54681880190935 25.72412573433111 60-61 25.801568061177594 24.054319220126747 24.491003307417643 25.65310941127802 62-63 25.673727360578575 24.10804083996581 24.3431101835764 25.87512161587922 64-65 25.537908916708492 24.06017582010998 24.415196012780726 25.986719250400807 66-67 25.592237231262743 24.104127098724664 24.37322328830621 25.93041238170638 68-69 25.51968220995998 24.013571872759286 24.52535773530295 25.941388181977786 70-71 25.39547676747666 23.878845897104775 24.570347050768806 26.155330284649757 72-73 25.52871338771659 24.00194449496131 24.452741739945736 26.016600377376363 74-75 25.38746774311143 24.132742808498055 24.371492560763095 26.108296887627425 76-77 25.469355264250176 23.96007048753605 24.315467066227544 26.255107181986226 78-79 25.371794209804634 24.053333464470448 24.383409679722188 26.191462646002734 80-81 25.367190429261317 24.220390066199386 24.3349635606295 26.0774559439098 82-83 25.453578017139993 24.062951006689957 24.506919840975193 25.976551135194853 84-85 25.435855323815122 24.036172324406014 24.18930459019806 26.33866776158081 86-87 25.189725017558786 24.169881337860506 24.478030826422508 26.1623628181582 88-89 25.1848641959373 24.017296539770054 24.428237902205506 26.369601362087142 90-91 25.207025857767835 24.037046743226874 24.34792105366147 26.40800634534382 92-93 25.26944024177847 23.947285965786985 24.48031584648592 26.302957945948624 94-95 25.16433181154898 23.91444296422994 24.541569463561792 26.379655760659286 96-97 25.068862395681972 23.923648588290156 24.480325556656478 26.52716345937139 98-99 24.839532792924363 23.89266973013153 24.555969699726354 26.711827777217756 100-101 24.887312354581717 23.94217033946436 24.491860673814752 26.67865663213917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 17.0 2 17.0 3 25.0 4 23.5 5 23.5 6 35.0 7 73.0 8 132.5 9 180.0 10 175.5 11 147.0 12 177.5 13 233.0 14 262.5 15 302.5 16 341.5 17 392.5 18 451.0 19 508.5 20 603.5 21 722.0 22 789.0 23 915.0 24 1127.0 25 1303.0 26 1539.0 27 1810.0 28 2144.0 29 2622.0 30 3085.5 31 3595.5 32 4439.5 33 5159.5 34 5773.5 35 7000.0 36 9149.5 37 12553.5 38 17355.5 39 23299.5 40 30263.5 41 38441.0 42 47463.0 43 57566.0 44 68552.0 45 79498.5 46 90354.5 47 101125.5 48 111320.0 49 119136.0 50 123474.0 51 127355.5 52 132835.5 53 136595.5 54 137400.0 55 132823.5 56 123004.0 57 111505.0 58 100785.5 59 90615.0 60 78269.5 61 65554.5 62 53532.0 63 42213.0 64 32762.5 65 24950.5 66 18608.0 67 14404.0 68 11294.5 69 8423.0 70 6201.0 71 4516.0 72 3258.0 73 2436.5 74 1780.0 75 1334.0 76 951.5 77 554.0 78 285.0 79 165.5 80 99.5 81 56.5 82 39.5 83 23.5 84 9.0 85 4.5 86 3.5 87 3.5 88 4.0 89 3.0 90 2.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0207345267569643 2 0.002868412792465417 3 0.003237208722925256 4 0.03315065641577889 5 0.002007888954725792 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.048866280332441E-5 24-25 5.941712212964078E-4 26-27 0.0018849569779058456 28-29 4.097732560664882E-5 30-31 0.007089077329950246 32-33 0.022271176467213633 34-35 0.013686426752620705 36-37 4.917279072797859E-4 38-39 4.917279072797859E-4 40-41 0.002069354943135765 42-43 0.008564261051789602 44-45 0.00831839709814971 46-47 0.008113510470116466 48-49 0.009834558145595716 50-51 0.18847520912778123 52-53 0.04921376805358523 54-55 0.017825136638892236 56-57 0.010756547971745314 58-59 0.019812536930814702 60-61 0.0537212738703166 62-63 0.0971162616877577 64-65 0.10180816546971898 66-67 0.10588540936758055 68-69 0.10400045238967469 70-71 0.09312097244110944 72-73 0.06943607824046641 74-75 0.23834461439107285 76-77 0.2883164629683811 78-79 0.007375918609196787 80-81 7.785691865263276E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.146598840997323E-5 90-91 0.006535883434260486 92-93 0.018624194488221887 94-95 0.0015161610474460061 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2440374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.71063111043245 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56919469652131 43.42287354857301 2 12.739290244330462 14.449063793099112 3 4.230666062273577 7.19771227227068 4 1.9540949932276508 4.432718412627052 5 1.1133650793392105 3.1569818152821663 6 0.7084335889760651 2.410542955839681 7 0.48108328537283884 1.9097775711121776 8 0.36599341114291917 1.660457386253994 9 0.26215867991351915 1.338046577007617 >10 1.4765311870465954 14.871916639596519 >50 0.07692991658838998 2.946053629708444 >100 0.02153565599260755 1.9056902540131684 >500 5.78559419841071E-4 0.21183259586602474 >1k 1.4463985495945264E-4 0.0863325487504175 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2293197681994644E-4 2 0.0 0.0 0.0 0.0 1.2293197681994644E-4 3 0.0 0.0 0.0 0.0 1.2293197681994644E-4 4 0.0 0.0 0.0 0.0 1.2293197681994644E-4 5 0.0 0.0 0.0 0.0 1.2293197681994644E-4 6 0.0 0.0 0.0 0.0 2.0488662803324408E-4 7 0.0 0.0 0.0 0.0 2.0488662803324408E-4 8 0.0 0.0 0.0 0.0 2.458639536398929E-4 9 0.0 0.0 0.0 4.097732560664882E-5 3.2781860485319055E-4 10-11 0.0 0.0 0.0 4.097732560664882E-5 3.2781860485319055E-4 12-13 0.0 0.0 0.0 4.097732560664882E-5 5.53193895689759E-4 14-15 0.0 0.0 0.0 4.097732560664882E-5 7.990578493296519E-4 16-17 0.0 0.0 0.0 8.195465121329764E-5 9.01501163346274E-4 18-19 0.0 0.0 0.0 8.195465121329764E-5 9.424784889529228E-4 20-21 0.0 0.0 0.0 8.195465121329764E-5 9.834558145595715E-4 22-23 0.0 0.0 0.0 8.195465121329764E-5 0.0011268764541828426 24-25 0.0 0.0 0.0 2.458639536398929E-4 0.0012293197681994645 26-27 0.0 0.0 0.0 6.556372097063811E-4 0.001454695059036033 28-29 0.0 0.0 0.0 0.0021308209315457384 0.0015161610474460061 30-31 2.048866280332441E-5 0.0 0.0 0.008482306400576305 0.0015161610474460061 32-33 4.097732560664882E-5 0.0 0.0 0.02751627414486468 0.0015981156986593038 34-35 4.097732560664882E-5 0.0 0.0 0.05298368200939692 0.0018439796522991968 36-37 4.097732560664882E-5 0.0 0.0 0.08742512418178525 0.0018439796522991968 38-39 4.097732560664882E-5 0.0 0.0 0.13881069049252287 0.0018849569779058456 40-41 8.195465121329764E-5 0.0 0.0 0.1942325233755154 0.0018849569779058456 42-43 8.195465121329764E-5 0.0 0.0 0.25914060713644715 0.0019054456407091701 44-45 8.195465121329764E-5 0.0 0.0 0.34175089555945115 0.001966911629119143 46-47 8.195465121329764E-5 0.0 0.0 0.43466698137252735 0.002110332268742414 48-49 8.195465121329764E-5 0.0 0.0 0.5240590171834316 0.002212775582759036 50-51 8.195465121329764E-5 0.0 0.0 0.6210318582315661 0.002253752908365685 52-53 1.0244331401662204E-4 0.0 0.0 0.7205043161417062 0.002458639536398929 54-55 1.2293197681994644E-4 0.0 0.0 0.8402810388899407 0.0025815715132188753 56-57 1.2293197681994644E-4 0.0 0.0 0.977923875602674 0.0026020601760222 58-59 1.2293197681994644E-4 0.0 0.0 1.1186605003987093 0.002663526164432173 60-61 1.2293197681994644E-4 0.0 0.0 1.2654207920589222 0.0027864581412521197 62-63 1.2293197681994644E-4 0.0 0.0 1.418757944479002 0.0030323220948920123 64-65 1.2293197681994644E-4 0.0 0.0 1.598320585287337 0.0031757427345152833 66-67 1.2293197681994644E-4 0.0 0.0 1.8080015604165591 0.003257697385728581 68-69 1.2293197681994644E-4 0.0 0.0 2.0264107058999974 0.0033396520369418786 70-71 1.2293197681994644E-4 0.0 0.0 2.256539366506937 0.0035240500021717983 72-73 1.2293197681994644E-4 0.0 0.0 2.50295651404252 0.003954311921041611 74-75 1.2293197681994644E-4 0.0 0.0 2.771501417405693 0.004077243897861557 76-77 1.2293197681994644E-4 0.0 0.0 3.0912065117887666 0.004118221223468206 78-79 1.2293197681994644E-4 0.0 0.0 3.4258273526926613 0.00417968721187818 80-81 1.2293197681994644E-4 0.0 0.0 3.7668816337167987 0.004302619188698125 82-83 1.2293197681994644E-4 0.0 0.0 4.127359167078489 0.0046714151191579656 84-85 1.2293197681994644E-4 0.0 0.0 4.528998424012057 0.00469190378196129 86-87 1.2293197681994644E-4 0.0 0.0 4.976655217601892 0.004814835758781236 88-89 1.2293197681994644E-4 0.0 0.0 5.459224692608592 0.004937767735601182 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2265 0.0 49.274754 1 GTATCAA 4115 0.0 41.548702 1 TATCAAC 5335 0.0 31.502947 2 CAACGCA 5355 0.0 31.193264 5 TCAACGC 5430 0.0 30.688858 4 ATCAACG 5510 0.0 30.243284 3 AACGCAG 5770 0.0 29.360945 6 GTACATG 10350 0.0 24.090385 1 GTGGTAT 1130 0.0 23.115805 1 TACATGG 10490 0.0 23.036932 2 ACGCAGA 7275 0.0 22.830357 7 TATAGCG 125 2.2715956E-4 22.778172 5 TATACCG 215 4.4718035E-8 22.071873 5 ACATGGG 11080 0.0 21.595547 3 CTAGTAC 620 0.0 21.440739 3 CGCAGAG 7770 0.0 21.436985 8 TAGATCG 160 5.0291965E-5 20.761356 5 GTACTAG 520 0.0 20.092968 1 CATGGGG 8815 0.0 19.44278 4 GCAGAGT 8535 0.0 19.12637 9 >>END_MODULE