FastQCFastQC Report
Sat 18 Jun 2016
SRR1930965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520898
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45580.18080858487729373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.059.3165471
GTATCAA44500.044.480591
ATCAACG54300.036.617713
TCAACGC55750.035.8303684
CAACGCA56850.034.5637785
TATCAAC56900.034.5127642
AACGCAG62000.031.200656
GTACATG55450.026.7950611
ACGCAGA71550.026.7922697
TACATGG54950.026.1228522
CGCAGAG75150.025.2285848
ACATGGG58450.024.38373
GCAGAGT81550.022.6032479
GTGGTAT9800.021.876731
CTAGTAC4700.021.2055853
CATGGGG42450.020.8878654
GAGTACT52500.020.16298512-13
AGAGTAC74250.019.56504410-11
GTATTAC4900.019.3329241
CAGAGTA78100.019.19783610-11
Produced by FastQC (version 0.11.5)