##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930965_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2520898 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.007186328046593 31.0 25.0 31.0 2.0 33.0 2 23.850125233151044 31.0 16.0 31.0 2.0 34.0 3 24.372575963010007 31.0 16.0 31.0 2.0 34.0 4 26.547371611227426 35.0 19.0 37.0 2.0 37.0 5 26.521684732980074 35.0 19.0 37.0 2.0 37.0 6 27.35725919890452 35.0 17.0 37.0 2.0 37.0 7 27.518562036226772 35.0 19.0 37.0 2.0 37.0 8 28.396386129069878 35.0 26.0 37.0 2.0 37.0 9 29.767048884960836 35.0 27.0 39.0 2.0 39.0 10-11 30.112912541483233 35.0 29.0 39.0 2.0 39.0 12-13 30.10851053870486 35.0 29.5 39.0 2.0 39.0 14-15 30.491488747263872 36.5 28.0 39.0 2.0 40.0 16-17 29.611627681881615 36.0 26.5 39.0 2.0 40.0 18-19 28.986389373945315 36.0 25.0 39.0 2.0 40.0 20-21 28.477105975727696 35.5 21.0 39.0 2.0 40.0 22-23 28.486709299622596 35.0 21.0 39.0 2.0 40.0 24-25 28.20736459785362 35.0 19.0 39.0 2.0 40.0 26-27 27.914899373159884 34.0 18.5 38.5 2.0 40.0 28-29 27.93977721431014 34.0 19.0 38.0 2.0 40.0 30-31 27.958415215530337 34.0 20.0 38.0 2.0 40.0 32-33 27.741035734091582 34.0 19.0 38.0 2.0 40.0 34-35 24.42457767033811 31.0 8.5 38.0 2.0 40.0 36-37 22.644561382491474 25.0 8.5 37.0 2.0 39.5 38-39 21.582337920852012 25.5 2.0 36.0 2.0 39.0 40-41 23.26956584518691 30.0 5.5 37.0 2.0 39.0 42-43 23.24582728059604 30.0 5.0 37.0 2.0 39.0 44-45 20.961639265055545 22.5 2.0 36.0 2.0 39.0 46-47 22.10449748462651 26.0 7.0 35.0 2.0 39.0 48-49 21.477778950199493 27.0 2.0 36.0 2.0 39.0 50-51 19.03141261566315 14.5 4.0 34.0 2.0 38.5 52-53 22.590867619395944 27.0 2.0 35.5 2.0 38.5 54-55 24.68590795819585 31.5 2.0 37.0 2.0 39.0 56-57 25.004438696051963 32.0 2.0 38.0 2.0 40.0 58-59 24.628375880341054 32.0 2.0 37.5 2.0 40.0 60-61 24.326982091302384 31.0 2.0 37.0 2.0 39.0 62-63 23.846529292339476 31.0 2.0 36.5 2.0 39.0 64-65 23.327862134842427 30.5 2.0 36.0 2.0 39.0 66-67 22.905400377167183 30.0 2.0 35.5 2.0 38.5 68-69 22.436333798511484 29.5 2.0 35.0 2.0 38.0 70-71 21.810097631875628 29.0 2.0 34.5 2.0 37.5 72-73 21.32782802001509 28.0 2.0 34.0 2.0 37.0 74-75 20.75854397916933 27.5 2.0 34.0 2.0 36.0 76-77 20.168852131264334 26.0 2.0 34.0 2.0 36.0 78-79 19.65094621043771 26.0 2.0 33.5 2.0 35.0 80-81 19.119635145888488 25.5 2.0 33.0 2.0 35.0 82-83 18.61474799853068 24.5 2.0 32.5 2.0 35.0 84-85 18.057019760418708 23.5 2.0 32.0 2.0 35.0 86-87 17.449484866107234 20.5 2.0 32.0 2.0 34.0 88-89 16.849179538402588 19.0 2.0 31.5 2.0 34.0 90-91 16.212080377706677 14.0 2.0 31.0 2.0 34.0 92-93 15.500145384700215 4.5 2.0 31.0 2.0 34.0 94-95 14.73939385885506 2.0 2.0 31.0 2.0 34.0 96-97 13.870873990141607 2.0 2.0 30.0 2.0 34.0 98-99 12.89036882888558 2.0 2.0 29.5 2.0 34.0 100-101 11.504569205100722 2.0 2.0 27.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.509529047229815 1101 2 7.75432324454178 1101 3 7.44076005616898 1101 4 9.2425813252561 1101 5 9.173572582852454 1101 6 8.38332051705947 1101 7 8.188279991651228 1101 8 7.028030680846211 1101 9 7.114338010247174 1101 10-11 6.476991937326293 1101 12-13 6.313395225496624 1101 14-15 6.93056864836171 1101 16-17 7.725313070878304 1101 18-19 8.276934689570432 1101 20-21 8.648532049584418 1101 22-23 8.543099283390564 1101 24-25 8.76447131486087 1101 26-27 8.664571799303982 1101 28-29 8.440692004219994 1101 30-31 8.142639057010555 1101 32-33 8.223782818272763 1101 34-35 11.022062622889873 1101 36-37 4.652801986252406 1101 38-39 8.362992156297484 1101 40-41 9.968710096555725 1101 42-43 10.461457492740745 1101 44-45 4.12609131384659 1101 46-47 6.006790180471491 1101 48-49 9.506737459668091 1101 50-51 -5.01961097156072 1101 52-53 5.676857006246973 1101 54-55 8.221792468600285 1101 56-57 8.640431458351667 1101 58-59 9.040226907965653 1101 60-61 8.929831745926784 1101 62-63 8.837887936818223 1101 64-65 8.776825987146129 1101 66-67 8.687836302877205 1101 68-69 8.682384181643993 1101 70-71 8.768304926973222 1101 72-73 8.788888329817915 1101 74-75 8.83072365358209 1101 76-77 8.732254721055881 1101 78-79 8.732086049496036 1101 80-81 8.61821347125316 1101 82-83 8.376526976541957 1101 84-85 8.556407717897027 1101 86-87 8.49491051979873 1101 88-89 8.314703441549776 1101 90-91 8.167284060774307 1101 92-93 8.27230020726227 1101 94-95 7.950819574410001 1101 96-97 7.797542617275363 1101 98-99 7.25969310363851 1101 100-101 7.055369423330333 1102 1 6.364615585586176 1102 2 7.708498525391494 1102 3 7.35697140173172 1102 4 9.17305427125201 1102 5 9.26227405628796 1102 6 8.479587772772181 1102 7 8.138966528366787 1102 8 7.174793115351076 1102 9 7.1504693806406365 1102 10-11 6.559678010516773 1102 12-13 6.229264396566091 1102 14-15 6.7266287192988194 1102 16-17 7.631955323856349 1102 18-19 8.137780766425351 1102 20-21 8.640472148196302 1102 22-23 8.311509706825522 1102 24-25 8.571687607425872 1102 26-27 8.352884164621909 1102 28-29 8.176358106179105 1102 30-31 8.079495044277976 1102 32-33 7.976530640115055 1102 34-35 10.609354378433036 1102 36-37 0.3778148733437128 1102 38-39 3.2533589017223896 1102 40-41 8.715586258786775 1102 42-43 9.85762240375487 1102 44-45 0.7191436333862917 1102 46-47 1.2501292292469124 1102 48-49 8.00377906890687 1102 50-51 -6.269321268380857 1102 52-53 4.4944166423729435 1102 54-55 7.351316111114421 1102 56-57 8.023504007548222 1102 58-59 8.405321110210267 1102 60-61 8.160914042823485 1102 62-63 8.249756640260618 1102 64-65 8.317697551044223 1102 66-67 8.262781062719252 1102 68-69 8.110558815746316 1102 70-71 8.145688184282971 1102 72-73 8.133470008681172 1102 74-75 8.142999069524073 1102 76-77 7.863823206545771 1102 78-79 8.04032305438561 1102 80-81 7.979233370015997 1102 82-83 7.822947954402139 1102 84-85 7.710295141463245 1102 86-87 7.819764523069388 1102 88-89 7.7867092267257245 1102 90-91 7.683582300199376 1102 92-93 7.766322750442571 1102 94-95 7.746845291164725 1102 96-97 7.37392512528792 1102 98-99 6.948495144242761 1102 100-101 6.4859698729086475 1103 1 6.434849215412889 1103 2 7.7198567433010865 1103 3 7.256388674862116 1103 4 9.099158730825447 1103 5 9.101810796251964 1103 6 8.305833957919685 1103 7 8.014519997165422 1103 8 7.0682829554533235 1103 9 7.120468076417559 1103 10-11 6.460650091448155 1103 12-13 6.2970521733874385 1103 14-15 6.801124376235986 1103 16-17 7.478292978515473 1103 18-19 8.090336261117159 1103 20-21 8.377399398633216 1103 22-23 8.325804687305652 1103 24-25 8.359311747725492 1103 26-27 8.355423226102982 1103 28-29 8.213024338104749 1103 30-31 8.01150519982832 1103 32-33 7.852463029190883 1103 34-35 10.432535465459491 1103 36-37 -4.0699873368796595 1103 38-39 -2.4320788390154497 1103 40-41 6.933976673616662 1103 42-43 9.29241420627811 1103 44-45 0.26145662953448934 1103 46-47 -0.022067691047666926 1103 48-49 7.6284349713857615 1103 50-51 -6.6527059881033175 1103 52-53 4.010293558494009 1103 54-55 7.190260038217048 1103 56-57 7.991222573598424 1103 58-59 8.152886647419749 1103 60-61 7.982247654391074 1103 62-63 8.077630148012048 1103 64-65 8.04151788127351 1103 66-67 7.7634566502717455 1103 68-69 7.75654102614358 1103 70-71 7.958043002517748 1103 72-73 7.762997097394425 1103 74-75 7.643821599542939 1103 76-77 7.576012475398311 1103 78-79 7.575498994078334 1103 80-81 7.391604186720485 1103 82-83 7.182848833448492 1103 84-85 7.217826073287156 1103 86-87 7.255913587071959 1103 88-89 7.168077269782474 1103 90-91 7.24134216664693 1103 92-93 7.141866481042252 1103 94-95 6.832648909949015 1103 96-97 6.832368230730006 1103 98-99 6.698941449378795 1103 100-101 6.322009208276569 1104 1 6.219602240165912 1104 2 7.392064535508879 1104 3 6.864700363173803 1104 4 8.88594971761643 1104 5 8.768117822558988 1104 6 7.880858932944658 1104 7 7.762181224826655 1104 8 6.5632280103983796 1104 9 6.69956897551846 1104 10-11 5.932256263054331 1104 12-13 5.678064494399759 1104 14-15 6.421217616329226 1104 16-17 7.13267637289886 1104 18-19 7.682151112932019 1104 20-21 8.21055957179339 1104 22-23 7.97465361615458 1104 24-25 7.903338831752578 1104 26-27 8.075050265730024 1104 28-29 7.843129788210202 1104 30-31 7.4239283322514495 1104 32-33 7.199356136083988 1104 34-35 10.204072816996838 1104 36-37 -4.175890322782646 1104 38-39 -2.6369051238417356 1104 40-41 6.259406799046786 1104 42-43 8.735087088950994 1104 44-45 -0.08649985842199825 1104 46-47 -0.8965088054887858 1104 48-49 7.148836791787584 1104 50-51 -6.883448578845908 1104 52-53 3.4236157918162426 1104 54-55 6.5424833704403795 1104 56-57 7.01932558170143 1104 58-59 7.305240959774061 1104 60-61 7.179761251904672 1104 62-63 7.206652237034138 1104 64-65 7.354737994493622 1104 66-67 7.165188252003347 1104 68-69 7.0779263075288625 1104 70-71 7.1584470429217895 1104 72-73 7.10451557891291 1104 74-75 7.068352624073963 1104 76-77 7.156998156383995 1104 78-79 6.787700126279464 1104 80-81 6.433682108798411 1104 82-83 6.503734614334274 1104 84-85 6.361997457458543 1104 86-87 6.339567710726083 1104 88-89 6.3496867713919745 1104 90-91 6.177252922557685 1104 92-93 6.127820526996297 1104 94-95 5.535907873207975 1104 96-97 5.402686801048576 1104 98-99 5.139720670158015 1104 100-101 4.766211672479031 1105 1 5.8374873302246115 1105 2 7.091074893952211 1105 3 6.711789803892927 1105 4 8.675952271357172 1105 5 8.729129392951027 1105 6 7.663971864815029 1105 7 7.6066983924099745 1105 8 6.465688518039148 1105 9 6.502217266441164 1105 10-11 5.710733706740033 1105 12-13 5.515924219553849 1105 14-15 6.032497073075955 1105 16-17 6.86278403212798 1105 18-19 7.661183572713512 1105 20-21 7.961337903250751 1105 22-23 7.716228806802224 1105 24-25 7.8038317002188435 1105 26-27 7.749880743850664 1105 28-29 7.49138673485707 1105 30-31 7.237964488604717 1105 32-33 7.210975365064083 1105 34-35 9.852879499755215 1105 36-37 -4.433782005245558 1105 38-39 -2.901396903209257 1105 40-41 5.720061533415208 1105 42-43 8.284388155402915 1105 44-45 -3.9400497620244046 1105 46-47 -5.2407147687006965 1105 48-49 5.4445809496248465 1105 50-51 -11.390701913540616 1105 52-53 0.2973929305566294 1105 54-55 5.384127492273514 1105 56-57 6.489217840883153 1105 58-59 6.945163107396596 1105 60-61 6.938315671467144 1105 62-63 6.885399519386599 1105 64-65 6.877813798633479 1105 66-67 6.555159440539462 1105 68-69 6.482566353050949 1105 70-71 6.453451012234474 1105 72-73 6.467557915178904 1105 74-75 6.229414639878716 1105 76-77 6.263982643137471 1105 78-79 6.199928779670177 1105 80-81 6.1768034723257905 1105 82-83 6.028252565173542 1105 84-85 5.91185525337864 1105 86-87 5.8036887023234485 1105 88-89 5.858494029936724 1105 90-91 5.737560541668245 1105 92-93 5.625160953458183 1105 94-95 5.23940382889154 1105 96-97 5.089346891628866 1105 98-99 4.894520997586975 1105 100-101 4.6255420515854 1106 1 5.872254043807814 1106 2 7.347671634680335 1106 3 6.8762762460368165 1106 4 8.439917202276984 1106 5 8.550699926923269 1106 6 7.432708073902713 1106 7 7.346316561437234 1106 8 6.552701408287621 1106 9 6.425925951875435 1106 10-11 5.755503148182402 1106 12-13 5.682297884771767 1106 14-15 6.109605136399917 1106 16-17 6.834123440682564 1106 18-19 7.218406112058304 1106 20-21 7.749522671400058 1106 22-23 7.512908677924489 1106 24-25 7.558602297659604 1106 26-27 7.462018083390912 1106 28-29 7.550335536257531 1106 30-31 7.133761815599783 1106 32-33 7.08262550697712 1106 34-35 9.860951004815622 1106 36-37 -0.5237483228683679 1106 38-39 0.7451736060092706 1106 40-41 7.178609708348706 1106 42-43 8.558613809754942 1106 44-45 -3.9015964779740635 1106 46-47 -5.07858209523534 1106 48-49 5.419662500732105 1106 50-51 -11.26369209770329 1106 52-53 0.44146015867051247 1106 54-55 5.389472190201481 1106 56-57 6.331389343022618 1106 58-59 6.745647500428127 1106 60-61 6.885903706809504 1106 62-63 7.03354275575634 1106 64-65 6.893142659135908 1106 66-67 6.827102245600511 1106 68-69 6.45953562230649 1106 70-71 6.53624952097179 1106 72-73 6.414836034035343 1106 74-75 6.212248744455234 1106 76-77 5.98696068560395 1106 78-79 5.860899204429039 1106 80-81 5.5888491364901896 1106 82-83 5.604064152782623 1106 84-85 5.620462589686049 1106 86-87 5.493216866850489 1106 88-89 5.188136687812191 1106 90-91 5.029146903164538 1106 92-93 4.951344775025499 1106 94-95 4.969279369698285 1106 96-97 4.743333365705043 1106 98-99 4.747958966515123 1106 100-101 4.354225144155871 1107 1 5.912273909581767 1107 2 7.273299579534619 1107 3 6.869455298161299 1107 4 8.355782210704742 1107 5 8.653829010595754 1107 6 7.267708517468666 1107 7 7.357860739110819 1107 8 6.41100426515138 1107 9 6.350747254603718 1107 10-11 5.814267662507593 1107 12-13 5.569417327613063 1107 14-15 5.934160100201947 1107 16-17 7.012786572776502 1107 18-19 7.421136933313186 1107 20-21 7.850322909231146 1107 22-23 7.666560546433605 1107 24-25 7.713892619050554 1107 26-27 7.570171423641877 1107 28-29 7.524125149438117 1107 30-31 7.474768964487424 1107 32-33 7.208132243697303 1107 34-35 9.91136165219265 1107 36-37 -0.5212290925400183 1107 38-39 0.8855162532308043 1107 40-41 7.586701597271819 1107 42-43 8.752325664329103 1107 44-45 -3.7581808905681484 1107 46-47 -4.9166921105558075 1107 48-49 5.666696838717396 1107 50-51 -11.261847311895805 1107 52-53 0.6328406754597324 1107 54-55 5.797369956722317 1107 56-57 6.879051127473488 1107 58-59 7.369370628554893 1107 60-61 7.357920302156749 1107 62-63 7.327126723787696 1107 64-65 7.276117003081932 1107 66-67 7.0052764583706235 1107 68-69 6.9677612787591805 1107 70-71 6.958699116429674 1107 72-73 6.799082639759039 1107 74-75 6.793722215722628 1107 76-77 6.819808214542892 1107 78-79 6.689081690451726 1107 80-81 6.735854742366389 1107 82-83 6.603791534158635 1107 84-85 6.573866983281555 1107 86-87 6.408239349165164 1107 88-89 6.224850367485805 1107 90-91 5.959857914465005 1107 92-93 5.952577165706424 1107 94-95 5.623126855775794 1107 96-97 5.368860162570774 1107 98-99 5.1064651659722795 1107 100-101 4.859091660707858 1108 1 5.969333486232237 1108 2 6.622732297066694 1108 3 6.778187574043212 1108 4 8.129596820949399 1108 5 8.39360298312268 1108 6 7.6548740975357425 1108 7 7.676131846630675 1108 8 6.62456702042849 1108 9 4.707838193211764 1108 10-11 5.753378421872821 1108 12-13 5.045399602572569 1108 14-15 5.689769832001854 1108 16-17 6.077202004335486 1108 18-19 6.775751753129512 1108 20-21 7.812912890377078 1108 22-23 7.772014021891952 1108 24-25 7.4801700284790655 1108 26-27 7.715970950629764 1108 28-29 7.523346081515495 1108 30-31 7.286023479686911 1108 32-33 7.436952222371172 1108 34-35 10.147161142284116 1108 36-37 -4.318738399662134 1108 38-39 -2.8601380863417134 1108 40-41 6.058700350172799 1108 42-43 8.76117600875719 1108 44-45 -3.6922248489637433 1108 46-47 -5.085862383847598 1108 48-49 5.537868004902567 1108 50-51 -11.401311170006931 1108 52-53 0.2325632143762313 1108 54-55 5.510980492864206 1108 56-57 6.840264503163912 1108 58-59 7.109667423362829 1108 60-61 6.987756223773992 1108 62-63 7.101887542740645 1108 64-65 7.0445249963225045 1108 66-67 6.910308576704825 1108 68-69 6.745634864242653 1108 70-71 6.794285898655932 1108 72-73 6.666989579606806 1108 74-75 6.636339610909118 1108 76-77 6.494377356590423 1108 78-79 6.582334810966504 1108 80-81 6.5659336269138 1108 82-83 6.393099873856599 1108 84-85 6.392790050554797 1108 86-87 6.360192461193769 1108 88-89 6.377374267142299 1108 90-91 6.326537494878906 1108 92-93 6.168549257253824 1108 94-95 6.011387890572813 1108 96-97 5.796197827543894 1108 98-99 5.695072473677355 1108 100-101 5.423649696932763 1109 1 4.998651292757565 1109 2 6.812631263250495 1109 3 6.4789067845551145 1109 4 8.198131556704102 1109 5 8.073218178107776 1109 6 5.811663554332242 1109 7 5.303271673988849 1109 8 3.81491167800133 1109 9 3.2123342371626435 1109 10-11 3.03612343474661 1109 12-13 3.527513251920272 1109 14-15 4.019574416479745 1109 16-17 3.2333391630235333 1109 18-19 4.48099165953041 1109 20-21 5.045045713454414 1109 22-23 6.535302981736681 1109 24-25 6.403239155643931 1109 26-27 5.2602708747174525 1109 28-29 5.171506381721326 1109 30-31 5.160320116849512 1109 32-33 4.94994261985433 1109 34-35 7.762672102614065 1109 36-37 -4.8799043580747075 1109 38-39 -3.4013678658829534 1109 40-41 4.540865064249445 1109 42-43 5.170996284389336 1109 44-45 -4.829101800575511 1109 46-47 -6.773741065008039 1109 48-49 2.8864530619150663 1109 50-51 -11.523848910950278 1109 52-53 -0.9778582946354213 1109 54-55 3.13520063075854 1109 56-57 4.758252200672896 1109 58-59 5.499713714224395 1109 60-61 5.517345005968249 1109 62-63 5.467022658839536 1109 64-65 5.096209154126214 1109 66-67 5.373175518847116 1109 68-69 5.44547169189039 1109 70-71 5.537459659602568 1109 72-73 5.4177970284051185 1109 74-75 5.50407609342972 1109 76-77 5.6980430606576 1109 78-79 5.8536851502241305 1109 80-81 5.755639486249059 1109 82-83 5.372771220478501 1109 84-85 5.040127249938109 1109 86-87 5.003503177352137 1109 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-13.158459653461664 2313 92-93 -12.471655199663065 2313 94-95 -11.918804081503978 2313 96-97 -11.029967393216184 2313 98-99 -10.030371913015095 2313 100-101 -8.713400534657675 2314 1 -22.070108049544377 2314 2 -20.759117615673272 2314 3 -21.25950876103532 2314 4 -23.23759340592669 2314 5 -23.181266126824962 2314 6 -24.094166042080314 2314 7 -24.277445814800387 2314 8 -25.260947813777445 2314 9 -26.60978833383885 2314 10-11 -27.01712768632962 2314 12-13 -27.053204236868968 2314 14-15 -27.33169613658453 2314 16-17 -26.411279671057184 2314 18-19 -25.746586815805912 2314 20-21 -25.193369832136014 2314 22-23 -25.180691039190076 2314 24-25 -24.920004491590745 2314 26-27 -24.644063953384194 2314 28-29 -24.68817224309183 2314 30-31 -24.738409330086213 2314 32-33 -24.548562611834758 2314 34-35 -21.382507269583247 2314 36-37 -19.608790755683078 2314 38-39 -18.625583112519724 2314 40-41 -20.254912215272228 2314 42-43 -20.21596186209796 2314 44-45 -18.000252772174914 2314 46-47 -19.03420444318442 2314 48-49 -18.52199237904159 2314 50-51 -16.190141885539216 2314 52-53 -19.544749176548724 2314 54-55 -21.49914167118466 2314 56-57 -21.79219622982038 2314 58-59 -21.391728737195635 2314 60-61 -21.105786533643112 2314 62-63 -20.647327116945217 2314 64-65 -20.168738528982903 2314 66-67 -19.757056170241075 2314 68-69 -19.306023076420523 2314 70-71 -18.67255528808054 2314 72-73 -18.235977261579933 2314 74-75 -17.66558010985877 2314 76-77 -17.100910601524763 2314 78-79 -16.600911262331923 2314 80-81 -16.090959915843616 2314 82-83 -15.623305012705355 2314 84-85 -15.103370507909425 2314 86-87 -14.523402652244279 2314 88-89 -13.942521020815816 2314 90-91 -13.335068089763325 2314 92-93 -12.648902749726977 2314 94-95 -11.918804081503978 2314 96-97 -11.082161683799908 2314 98-99 -10.157981627544283 2314 100-101 -8.864657458390099 2315 1 -22.070108049544377 2315 2 -20.759117615673272 2315 3 -21.25950876103532 2315 4 -16.4492913544208 2315 5 -23.181266126824962 2315 6 -24.094166042080314 2315 7 -24.277445814800387 2315 8 -25.260947813777445 2315 9 -20.38063076072667 2315 10-11 -18.450781112780952 2315 12-13 -13.819900002909998 2315 14-15 -13.666047948020584 2315 16-17 -20.811323320118728 2315 18-19 -25.746586815805912 2315 20-21 -25.193369832136014 2315 22-23 -25.180691039190076 2315 24-25 -24.920004491590745 2315 26-27 -24.644063953384194 2315 28-29 -24.68817224309183 2315 30-31 -24.738409330086213 2315 32-33 -24.548562611834758 2315 34-35 -21.382507269583247 2315 36-37 -19.608790755683078 2315 38-39 -18.625583112519724 2315 40-41 -20.254912215272228 2315 42-43 -20.21596186209796 2315 44-45 -18.000252772174914 2315 46-47 -19.03420444318442 2315 48-49 -18.52199237904159 2315 50-51 -16.190141885539216 2315 52-53 -19.544749176548724 2315 54-55 -21.49914167118466 2315 56-57 -21.79219622982038 2315 58-59 -21.391728737195635 2315 60-61 -21.105786533643112 2315 62-63 -20.647327116945217 2315 64-65 -20.168738528982903 2315 66-67 -19.757056170241075 2315 68-69 -19.306023076420523 2315 70-71 -18.67255528808054 2315 72-73 -18.235977261579933 2315 74-75 -17.66558010985877 2315 76-77 -17.100910601524763 2315 78-79 -16.600911262331923 2315 80-81 -16.090959915843616 2315 82-83 -15.623305012705355 2315 84-85 -15.103370507909425 2315 86-87 -14.523402652244279 2315 88-89 -13.942521020815816 2315 90-91 -13.335068089763325 2315 92-93 -12.648902749726977 2315 94-95 -11.918804081503978 2315 96-97 -11.082161683799908 2315 98-99 -10.157981627544283 2315 100-101 -8.864657458390099 2316 1 -22.070108049544377 2316 2 -20.759117615673272 2316 3 -21.25950876103532 2316 4 -23.23759340592669 2316 5 -23.181266126824962 2316 6 -24.094166042080314 2316 7 -24.277445814800387 2316 8 -25.260947813777445 2316 9 -26.60978833383885 2316 10-11 -27.01712768632962 2316 12-13 -27.053204236868968 2316 14-15 -27.33169613658453 2316 16-17 -26.411279671057184 2316 18-19 -25.746586815805912 2316 20-21 -25.193369832136014 2316 22-23 -25.180691039190076 2316 24-25 -24.920004491590745 2316 26-27 -24.644063953384194 2316 28-29 -24.68817224309183 2316 30-31 -24.738409330086213 2316 32-33 -24.548562611834758 2316 34-35 -21.382507269583247 2316 36-37 -19.608790755683078 2316 38-39 -18.625583112519724 2316 40-41 -20.254912215272228 2316 42-43 -20.21596186209796 2316 44-45 -18.000252772174914 2316 46-47 -19.03420444318442 2316 48-49 -18.52199237904159 2316 50-51 -16.190141885539216 2316 52-53 -19.544749176548724 2316 54-55 -21.49914167118466 2316 56-57 -21.79219622982038 2316 58-59 -21.391728737195635 2316 60-61 -21.105786533643112 2316 62-63 -20.647327116945217 2316 64-65 -20.168738528982903 2316 66-67 -19.757056170241075 2316 68-69 -19.306023076420523 2316 70-71 -18.67255528808054 2316 72-73 -18.235977261579933 2316 74-75 -17.66558010985877 2316 76-77 -17.100910601524763 2316 78-79 -16.600911262331923 2316 80-81 -16.090959915843616 2316 82-83 -15.623305012705355 2316 84-85 -15.103370507909425 2316 86-87 -14.523402652244279 2316 88-89 -13.942521020815816 2316 90-91 -13.335068089763325 2316 92-93 -12.648902749726977 2316 94-95 -11.918804081503978 2316 96-97 -11.082161683799908 2316 98-99 -10.157981627544283 2316 100-101 -8.864657458390099 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 303865.0 3 17524.0 4 43495.0 5 18920.0 6 13881.0 7 17053.0 8 22866.0 9 30195.0 10 34732.0 11 35838.0 12 34257.0 13 32943.0 14 31475.0 15 33986.0 16 38192.0 17 41018.0 18 42183.0 19 45172.0 20 49431.0 21 53290.0 22 58241.0 23 63923.0 24 70448.0 25 77593.0 26 86238.0 27 95341.0 28 106510.0 29 117568.0 30 127687.0 31 131565.0 32 131824.0 33 133595.0 34 130401.0 35 115055.0 36 87000.0 37 41429.0 38 6116.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.438774672004335 19.531427632644853 13.676859882575531 24.352937812775277 2 15.409451244667924 20.513109694920022 40.49199865053361 23.585440409878444 3 18.479678136174375 25.293746328025534 30.303028893409355 25.92354664239074 4 12.59893452656351 16.705140176071236 38.400693658857904 32.295231638507346 5 13.321865471646573 37.532736315469556 35.275487076507375 13.869911136376501 6 31.005343463313572 37.07320530931007 17.8819374922039 14.039513735172454 7 26.81908275640623 32.54305463456432 22.839945822975025 17.79791678605442 8 25.302931849334282 35.41083482456214 20.98279303805604 18.303440288047536 9 26.206473793566882 15.837104072398189 20.867196766682774 37.08922536735215 10-11 24.68612364723661 26.11867917549487 28.88776482776374 20.30743234950478 12-13 25.065373127496194 24.081077391646954 28.83972609906144 22.013823381795415 14-15 22.3574111850374 25.387524584642946 26.512451039484052 25.742613190835602 16-17 22.0607190067479 28.482442385730188 27.15602580486363 22.300812802658285 18-19 21.989034533627773 27.53267942383148 29.014275047615595 21.464010994925157 20-21 22.71633107898475 26.7654631460557 28.89341766786801 21.624788107091536 22-23 22.513823564710627 26.599515526724964 28.69227174056565 22.194389167998757 24-25 22.274123697187065 26.748899374768577 28.737323079333876 22.239653848710486 26-27 22.2000019175511 27.01751459958746 28.52883472872338 22.253648754138062 28-29 22.203624547355215 27.12305762125507 28.57845512258672 22.094862708803 30-31 22.434627924729444 26.89997161814973 28.467018290001388 22.198382167119433 32-33 22.295525494276795 27.0101844266832 28.31454380410477 22.379746274935243 34-35 22.620230505619055 27.012712270020867 28.297771523749354 22.069285700610724 36-37 23.160527650938608 26.98376458650431 27.876179604261797 21.97952815829528 38-39 22.876753932452676 27.009500182700645 27.937423838158075 22.176322046688604 40-41 22.848662797421657 26.987252261714534 28.05878451578185 22.10530042508196 42-43 22.91574599040581 27.079636886853166 27.668013798601105 22.33660332413992 44-45 23.19375369281608 27.242438974798766 27.447902983745067 22.115904348640086 46-47 22.63404279935743 27.178481953338107 27.694828744721278 22.49264650258318 48-49 23.086362107561165 27.291603783231714 27.197281710807246 22.424752398399875 50-51 23.416084201725575 27.3836903928673 26.942030233469268 22.25819517193786 52-53 22.494071188659607 27.373336740935304 27.55044006463932 22.582152005765767 54-55 22.265391332745008 27.31662217887762 27.699271296021728 22.71871519235565 56-57 22.28910728185474 27.522339870658545 27.5104294054774 22.67812344200932 58-59 22.323549135052893 27.369942545627307 27.65446314854257 22.652045170777228 60-61 22.30149722598008 27.39717895902276 27.696512994151828 22.604810820845334 62-63 22.275894185875483 27.384677117257873 27.626389809096075 22.71303888777057 64-65 22.11702795194122 27.283831838290084 27.61461885941922 22.98452135034947 66-67 22.110597822426065 27.419458497492094 27.562041687031225 22.907901993050615 68-69 22.305305860925174 27.275919581720714 27.56964851582781 22.8491260415263 70-71 22.163289277494968 27.17204150243629 27.485729876543967 23.178939343524775 72-73 22.250727018163253 27.28258322446203 27.424606394959195 23.042083362415518 74-75 22.22471241553425 27.504177828720856 27.252412712517188 23.01869704322771 76-77 22.147376917328206 27.30123751459447 27.340039248688004 23.21134631938932 78-79 22.113611116241884 27.414262606965593 27.297324991578204 23.17480128521432 80-81 22.256043535175117 27.379675640783795 27.162632952937 23.20164787110408 82-83 22.31490291356826 27.29733654390075 27.28266358614023 23.105096956390756 84-85 22.314865195200156 27.34800642710174 27.068612407268315 23.26851597042979 86-87 22.078489818780074 27.410199413259534 27.158658672008002 23.352652095952383 88-89 22.135185130655255 27.275825712124657 27.059857893702873 23.52913126351722 90-91 22.11753888045153 27.24024794755454 27.173418903115476 23.468794268878455 92-93 22.227511927544068 27.057294874355765 27.189112094519842 23.52608110358033 94-95 21.98039016451169 26.98888247500703 27.29695319199629 23.733774168484985 96-97 22.035373899294207 26.851628773120357 27.196533121522805 23.91646420606263 98-99 21.875766595743524 26.911099381281733 27.119167206299284 24.093966816675465 100-101 22.07983618337973 27.008462928466276 26.943469137869684 23.968231750284307 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24111.0 1 25668.0 2 19037.5 3 12142.0 4 16086.0 5 20191.0 6 21619.5 7 20733.0 8 18951.5 9 17160.5 10 15340.5 11 13334.5 12 11492.5 13 9870.0 14 8468.0 15 7485.0 16 6828.0 17 6424.0 18 6316.5 19 6501.5 20 7039.5 21 7825.0 22 8928.5 23 10485.5 24 12297.5 25 14728.5 26 17936.5 27 21553.0 28 25542.5 29 30286.0 30 35324.0 31 40412.0 32 46009.0 33 50611.0 34 54387.5 35 57988.5 36 61984.0 37 66247.5 38 69144.5 39 70867.0 40 72528.5 41 74187.0 42 75605.0 43 77340.0 44 79007.5 45 80570.5 46 81712.5 47 81943.5 48 81339.0 49 79991.0 50 77787.0 51 75341.5 52 73009.0 53 70136.5 54 65945.0 55 60444.5 56 54708.5 57 48445.0 58 42036.0 59 36264.5 60 30941.0 61 25751.5 62 20970.5 63 16796.0 64 12921.0 65 9811.0 66 7476.5 67 5669.5 68 4250.5 69 3191.0 70 2437.5 71 1884.5 72 1423.0 73 1071.0 74 831.5 75 626.5 76 432.5 77 290.0 78 191.0 79 110.5 80 62.5 81 40.5 82 26.5 83 15.0 84 9.0 85 7.0 86 5.0 87 5.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.592298458723835 2 18.750500813598965 3 14.723681799104924 4 19.66342946045417 5 19.403125394204764 6 13.817972801755566 7 12.78036636151086 8 10.198944979130452 9 2.479037231970512 10-11 5.461327669743084 12-13 2.942364189269062 14-15 6.182499252250587 16-17 9.748331745274898 18-19 12.472757723636576 20-21 12.865593927243388 22-23 10.923349536554037 24-25 13.171040637106302 26-27 11.045845567730229 28-29 11.043029111054869 30-31 11.737265847329008 32-33 10.414860101440041 34-35 17.120010408989177 36-37 21.813575955869695 38-39 20.91316269043809 40-41 15.965858198150023 42-43 15.802464042575304 44-45 21.180230219548747 46-47 10.855972752566743 48-49 19.504180653084735 50-51 19.1516078794144 52-53 9.431857219133818 54-55 10.840482240852268 56-57 10.408195809588488 58-59 11.327669743083616 60-61 10.78605719073124 62-63 10.730263580676409 64-65 11.683971346718511 66-67 10.193054221154526 68-69 11.095887259222707 70-71 9.930290713864663 72-73 9.350140307144517 74-75 9.465357186209042 76-77 9.47216031747417 78-79 9.799523820479845 80-81 9.78583822114183 82-83 8.486380646896464 84-85 8.394409452504624 86-87 8.533010855655405 88-89 9.740933587951595 90-91 7.549869133935605 92-93 9.343357010081329 94-95 9.427434192101385 96-97 9.030650982308686 98-99 8.878205306204375 100-101 9.028608059508953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2520898.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.82718706825023 #Duplication Level Percentage of deduplicated Percentage of total 1 98.81952569099005 95.68416700077252 2 0.8368924544760274 1.6206788449111478 3 0.1585439145418833 0.4605408381563882 4 0.0561761206766735 0.21757502982115454 5 0.029232288797662678 0.14152401479221996 6 0.02220990083259608 0.129031333361104 7 0.013161467019933048 0.0892071480462164 8 0.011155063216066323 0.08640907142241686 9 0.008645597605832656 0.07534160070270972 >10 0.040343632091817974 0.6737855827856808 >50 0.0022225916906831464 0.15739222698272823 >100 0.0017272973290291735 0.3015000438565372 >500 4.099518295036684E-5 0.03659831285054592 >1k 1.2298554885110052E-4 0.32624895153865174 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4558 0.18080858487729373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.933680775660101E-5 2 0.0 0.0 0.0 0.0 7.933680775660101E-5 3 0.0 0.0 0.0 0.0 7.933680775660101E-5 4 0.0 0.0 0.0 0.0 7.933680775660101E-5 5 0.0 0.0 0.0 0.0 7.933680775660101E-5 6 0.0 0.0 0.0 3.966840387830051E-5 7.933680775660101E-5 7 0.0 0.0 0.0 3.966840387830051E-5 7.933680775660101E-5 8 0.0 0.0 0.0 3.966840387830051E-5 7.933680775660101E-5 9 0.0 0.0 0.0 7.933680775660101E-5 7.933680775660101E-5 10-11 0.0 0.0 0.0 7.933680775660101E-5 7.933680775660101E-5 12-13 0.0 0.0 0.0 7.933680775660101E-5 1.7850781745235229E-4 14-15 0.0 0.0 0.0 7.933680775660101E-5 2.3801042326980308E-4 16-17 0.0 0.0 0.0 7.933680775660101E-5 3.1734723102640406E-4 18-19 0.0 0.0 0.0 7.933680775660101E-5 3.5701563490470457E-4 20-21 0.0 0.0 0.0 7.933680775660101E-5 3.768498368438548E-4 22-23 0.0 0.0 0.0 1.3883941357405177E-4 4.5618664460045583E-4 24-25 0.0 0.0 0.0 1.9834201939150254E-4 4.760208465396061E-4 26-27 0.0 0.0 0.0 2.9751302908725383E-4 5.156892504179066E-4 28-29 0.0 0.0 0.0 0.0011900521163490153 5.156892504179066E-4 30-31 0.0 0.0 0.0 0.0038280009742559995 5.156892504179066E-4 32-33 0.0 0.0 0.0 0.011166655691741594 5.156892504179066E-4 34-35 0.0 0.0 0.0 0.02015154917017666 5.553576542962072E-4 36-37 0.0 0.0 0.0 0.034809024403208697 5.553576542962072E-4 38-39 0.0 0.0 0.0 0.0581737142875277 5.553576542962072E-4 40-41 0.0 0.0 0.0 0.08401767941424049 5.553576542962072E-4 42-43 0.0 0.0 0.0 0.11095252564760652 5.553576542962072E-4 44-45 0.0 0.0 0.0 0.1452656950023365 5.751918562353574E-4 46-47 0.0 0.0 0.0 0.18701669008424776 5.950260581745077E-4 48-49 0.0 0.0 0.0 0.23085027636976982 6.743628659311087E-4 50-51 0.0 0.0 0.0 0.2840456059705708 6.743628659311087E-4 52-53 0.0 0.0 0.0 0.3532868049401443 7.338654717485595E-4 54-55 0.0 0.0 0.0 0.43242527067735387 8.132022795051604E-4 56-57 0.0 0.0 0.0 0.5264790562728043 8.925390872617615E-4 58-59 0.0 0.0 0.0 0.6281491754128885 9.123732892009118E-4 60-61 0.0 0.0 0.0 0.7393992140895824 9.123732892009118E-4 62-63 0.0 0.0 0.0 0.8506492527662761 9.123732892009118E-4 64-65 0.0 0.0 0.0 0.9724114184707195 9.123732892009118E-4 66-67 0.0 0.0 0.0 1.1076608414937852 9.32207491140062E-4 68-69 0.0 0.0 0.0 1.2586982892604144 9.520416930792122E-4 70-71 0.0 0.0 0.0 1.4243932122600755 9.718758950183625E-4 72-73 0.0 0.0 0.0 1.6003424176622776 0.0011107153085924142 74-75 0.0 0.0 0.0 1.7801592924426137 0.001170217914409865 76-77 0.0 0.0 0.0 1.9802665558067005 0.0011900521163490153 78-79 0.0 0.0 0.0 2.206673970942101 0.0011900521163490153 80-81 0.0 0.0 0.0 2.4455372648952873 0.001249554722166466 82-83 0.0 0.0 0.0 2.702370345805344 0.001309057327983917 84-85 0.0 0.0 0.0 2.965034682085511 0.001309057327983917 86-87 0.0 0.0 0.0 3.2448556030430424 0.0013685599338013675 88-89 0.0 0.0 0.0 3.5510758467815835 0.0014280625396188183 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2135 0.0 59.316547 1 GTATCAA 4450 0.0 44.48059 1 ATCAACG 5430 0.0 36.61771 3 TCAACGC 5575 0.0 35.830368 4 CAACGCA 5685 0.0 34.563778 5 TATCAAC 5690 0.0 34.512764 2 AACGCAG 6200 0.0 31.20065 6 GTACATG 5545 0.0 26.795061 1 ACGCAGA 7155 0.0 26.792269 7 TACATGG 5495 0.0 26.122852 2 CGCAGAG 7515 0.0 25.228584 8 ACATGGG 5845 0.0 24.3837 3 GCAGAGT 8155 0.0 22.603247 9 GTGGTAT 980 0.0 21.87673 1 CTAGTAC 470 0.0 21.205585 3 CATGGGG 4245 0.0 20.887865 4 GAGTACT 5250 0.0 20.162985 12-13 AGAGTAC 7425 0.0 19.565044 10-11 GTATTAC 490 0.0 19.332924 1 CAGAGTA 7810 0.0 19.197836 10-11 >>END_MODULE