FastQCFastQC Report
Sat 18 Jun 2016
SRR1930965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520898
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.20480796922366556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43220.1714468415620148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.12209934713740898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.055.048521
GTATCAA59600.041.9161151
TCAACGC78750.030.9311644
ATCAACG78350.030.9072723
CAACGCA78900.030.6814735
TATCAAC80700.030.1843592
AACGCAG83800.029.2833616
ACGCAGA95400.025.1256567
GTACATG74900.024.793421
CGCAGAG99300.024.043258
TACATGG76000.023.679022
ACATGGG76400.022.8709353
GTGGTAT15250.021.4892251
GCAGAGT108850.021.1925039
CATGGGG53650.021.06384
AGAGTAC101250.020.27521710-11
GAGTACA57350.019.8755591
GAGTACT72250.019.08458512-13
AGTACTT77800.019.06535312-13
GGACCGT3851.2732926E-1118.4928976
Produced by FastQC (version 0.11.5)