##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930965_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2520898 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95411198707762 31.0 31.0 33.0 28.0 34.0 2 31.07322866692742 31.0 31.0 34.0 28.0 34.0 3 31.136697319764625 31.0 31.0 34.0 28.0 34.0 4 35.04569562116357 35.0 35.0 37.0 32.0 37.0 5 34.82488224434309 35.0 35.0 37.0 32.0 37.0 6 34.87872020208672 35.0 35.0 37.0 32.0 37.0 7 34.752150225832224 35.0 35.0 37.0 32.0 37.0 8 34.72847215555726 35.0 35.0 37.0 31.0 37.0 9 36.18857169151628 38.0 35.0 39.0 32.0 39.0 10-11 36.090092498784166 37.0 35.0 39.0 30.5 39.0 12-13 35.97915623718215 37.0 35.0 39.0 30.0 39.0 14-15 36.91351236741828 38.0 36.0 40.0 31.0 41.0 16-17 36.865709362298674 38.0 36.0 40.0 31.0 41.0 18-19 36.856498755602175 38.5 36.0 40.0 31.0 41.0 20-21 36.81532453911265 39.0 35.5 40.0 31.0 41.0 22-23 36.66844017489006 38.0 35.0 40.0 30.0 41.0 24-25 36.53012160745892 38.0 35.0 40.0 30.0 41.0 26-27 36.20351854775561 38.0 34.5 40.0 29.5 41.0 28-29 35.98504679681605 38.0 34.0 40.0 29.0 41.0 30-31 35.69874544705894 38.0 34.0 40.0 28.0 41.0 32-33 35.42097141574153 38.0 34.0 40.0 27.0 41.0 34-35 35.608382013076294 38.0 34.0 40.0 28.0 41.0 36-37 35.64020936190199 38.0 34.0 40.0 28.0 41.0 38-39 35.575249573763 38.0 34.0 40.0 27.5 41.0 40-41 35.445640204403354 38.0 34.0 40.0 27.0 41.0 42-43 35.26593301275974 38.0 34.0 40.0 27.0 41.0 44-45 35.02978224426375 38.0 33.0 40.0 26.5 41.0 46-47 34.76094252920983 38.0 33.0 40.0 26.0 41.0 48-49 34.51187275328078 38.0 33.0 40.0 24.5 41.0 50-51 34.20868654741287 37.0 33.0 40.0 24.0 41.0 52-53 33.96218688737109 37.0 32.5 40.0 24.0 41.0 54-55 33.74297631240931 37.0 32.0 40.0 23.0 41.0 56-57 33.443701807847845 36.5 32.0 39.5 23.0 41.0 58-59 33.16382158262651 36.0 31.0 39.0 22.5 40.5 60-61 32.813642003762155 36.0 31.0 39.0 22.0 40.0 62-63 32.48965091011219 35.5 31.0 39.0 21.0 40.0 64-65 32.14615644901142 35.0 30.5 38.5 20.0 40.0 66-67 31.780112682068058 35.0 30.0 38.0 19.0 40.0 68-69 31.37825251160499 34.5 29.5 38.0 18.0 40.0 70-71 30.949175650899004 34.0 29.0 37.0 17.5 39.5 72-73 30.506670043770118 34.0 29.0 36.5 14.0 39.0 74-75 29.968370993193695 34.0 28.5 36.0 11.0 39.0 76-77 28.71672356438063 32.0 26.5 35.0 8.0 37.0 78-79 29.489482716079745 33.5 28.5 35.0 9.0 37.0 80-81 29.488769279835996 34.0 29.0 35.0 7.5 37.0 82-83 29.193118880652847 33.5 29.0 35.0 7.0 37.0 84-85 28.85367357187796 33.0 28.5 35.0 4.5 36.0 86-87 28.471427443712518 33.0 27.5 35.0 2.0 36.0 88-89 28.08921622374249 33.0 27.0 35.0 2.0 35.5 90-91 27.695807010041662 33.0 26.0 35.0 2.0 35.0 92-93 27.282533644756747 32.5 26.0 35.0 2.0 35.0 94-95 26.777463229373026 32.0 25.0 34.0 2.0 35.0 96-97 26.217739075519916 32.0 25.0 34.0 2.0 35.0 98-99 25.61533290914587 32.0 23.5 34.0 2.0 35.0 100-101 24.30167979823063 31.0 18.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4786119994062794 1101 2 0.6299097947970473 1101 3 0.5112123958627137 1101 4 0.401097680242664 1101 5 0.5357243156496168 1101 6 0.44194539248676534 1101 7 0.5150013927081929 1101 8 0.5378301149647555 1101 9 0.7883643882345339 1101 10-11 0.7420406311458194 1101 12-13 0.690688944374962 1101 14-15 0.8997752682854951 1101 16-17 0.8650065518876175 1101 18-19 0.8592425055395836 1101 20-21 0.8426247618497484 1101 22-23 0.8365856559146465 1101 24-25 0.8595690076685116 1101 26-27 0.9241308287807684 1101 28-29 0.8267954227175238 1101 30-31 0.9069258775172102 1101 32-33 0.8708723386765769 1101 34-35 0.7098856843484285 1101 36-37 0.884255996685404 1101 38-39 0.9773947559798302 1101 40-41 0.9341879280910277 1101 42-43 0.9859517823223527 1101 44-45 1.0580936671199268 1101 46-47 1.1090385170337171 1101 48-49 1.239250769553692 1101 50-51 1.1428994957117098 1101 52-53 1.1368508001150985 1101 54-55 1.1808117876154611 1101 56-57 1.3165944256253894 1101 58-59 1.1977738205372432 1101 60-61 1.1101129324427177 1101 62-63 1.0483600606672567 1101 64-65 1.0901873936080264 1101 66-67 1.162213926621618 1101 68-69 1.0212848198643485 1101 70-71 1.0079836939801972 1101 72-73 1.1226224200263992 1101 74-75 1.1828308359711208 1101 76-77 1.2346592633663178 1101 78-79 1.3183524651569734 1101 80-81 1.272661520942787 1101 82-83 1.3750174116903935 1101 84-85 1.3380234266187117 1101 86-87 1.2009750207843446 1101 88-89 1.1517451437510076 1101 90-91 1.2284899758661645 1101 92-93 1.2825250667308197 1101 94-95 1.3547998603570797 1101 96-97 1.497117947062499 1101 98-99 1.4125886533745735 1101 100-101 1.5671487389834908 1102 1 0.5388839341244456 1102 2 0.5888112540123096 1102 3 0.6351537401128162 1102 4 0.4590943767718372 1102 5 0.5290137986892489 1102 6 0.4760872967954981 1102 7 0.4831928381407664 1102 8 0.47268535566963976 1102 9 0.6661058861726019 1102 10-11 0.7463868625091052 1102 12-13 0.811771029335425 1102 14-15 0.9145754260754444 1102 16-17 0.7815453731392239 1102 18-19 0.9315184068839386 1102 20-21 0.8180015298082708 1102 22-23 0.9138779515534168 1102 24-25 0.8978455489236126 1102 26-27 0.8988097453626622 1102 28-29 0.8795096082260088 1102 30-31 0.9788693465991258 1102 32-33 1.0993599547629458 1102 34-35 0.9955217392166773 1102 36-37 1.0539632648681732 1102 38-39 1.2510241095605963 1102 40-41 1.250364851104841 1102 42-43 1.134251438301959 1102 44-45 1.1550030554855795 1102 46-47 1.1909824715180193 1102 48-49 1.1374829135828293 1102 50-51 1.0838159422381466 1102 52-53 1.2700036799868926 1102 54-55 1.2299086999094087 1102 56-57 1.263833921791388 1102 58-59 1.2739396066429762 1102 60-61 1.1885079802446015 1102 62-63 1.052838923102854 1102 64-65 1.1597431615556104 1102 66-67 1.1125495721110639 1102 68-69 0.9896982856099754 1102 70-71 0.9680697742807922 1102 72-73 0.9378521247376703 1102 74-75 1.1058191849467178 1102 76-77 1.271621799874044 1102 78-79 1.2188800155020907 1102 80-81 1.122773710421999 1102 82-83 1.3377185118446278 1102 84-85 1.3602569065298908 1102 86-87 1.4221981800838321 1102 88-89 1.4647348190373286 1102 90-91 1.4220352864689936 1102 92-93 1.4905023813481328 1102 94-95 1.44367703578013 1102 96-97 1.6365793486857925 1102 98-99 1.503537847469893 1102 100-101 1.613032082435069 1103 1 0.5081129643911133 1103 2 0.5347381653512251 1103 3 0.49451919211306006 1103 4 0.39386305107255026 1103 5 0.34205220371137557 1103 6 0.4086172287150589 1103 7 0.526182465219911 1103 8 0.5344530126763658 1103 9 0.6827922900817924 1103 10-11 0.626252962273476 1103 12-13 0.6114755793349858 1103 14-15 0.6649215668793715 1103 16-17 0.7006510239030632 1103 18-19 0.7381563806429767 1103 20-21 0.8185246102092734 1103 22-23 0.709933980976686 1103 24-25 0.7157292296150217 1103 26-27 0.7394994185243675 1103 28-29 0.7008588704704906 1103 30-31 0.8075048684187749 1103 32-33 0.8045198090377568 1103 34-35 0.9043887171579144 1103 36-37 1.0162573063076863 1103 38-39 1.0011311428008938 1103 40-41 0.9543169948128352 1103 42-43 0.9603565934610359 1103 44-45 0.974517536709115 1103 46-47 1.0539804456248376 1103 48-49 0.9887501836700423 1103 50-51 1.021093767837435 1103 52-53 0.8976429492741786 1103 54-55 1.0627253870088964 1103 56-57 1.0938007945253077 1103 58-59 1.0722816782709046 1103 60-61 1.0892270418995693 1103 62-63 0.884467897722665 1103 64-65 0.8029524198004765 1103 66-67 0.9729898778209503 1103 68-69 1.007874460062851 1103 70-71 0.9310604447383994 1103 72-73 0.9512230196955471 1103 74-75 1.031593415237733 1103 76-77 1.1965032459965919 1103 78-79 1.1785670019642538 1103 80-81 1.0420318683373289 1103 82-83 1.0736724640752797 1103 84-85 1.057268670622019 1103 86-87 1.2300569066404243 1103 88-89 1.2454625755127964 1103 90-91 1.1763914784854101 1103 92-93 1.312877458514663 1103 94-95 1.2421950905748886 1103 96-97 1.0833522660589168 1103 98-99 1.177263143311162 1103 100-101 1.478403046951506 1104 1 0.3764668327449847 1104 2 0.5080981387111976 1104 3 0.43578681338068037 1104 4 0.2542337914432906 1104 5 0.36049598215515033 1104 6 0.29453131462913973 1104 7 0.35809277713022425 1104 8 0.38468722291057134 1104 9 0.45421198150148 1104 10-11 0.43474225076276696 1104 12-13 0.42659601445541995 1104 14-15 0.5026238645816647 1104 16-17 0.45429293754497735 1104 18-19 0.6144400969266854 1104 20-21 0.49101434269901034 1104 22-23 0.43900491004761477 1104 24-25 0.4097263436121352 1104 26-27 0.6159962550212015 1104 28-29 0.32564519525681135 1104 30-31 0.35159411250802464 1104 32-33 0.6203528648708101 1104 34-35 0.5486000613692639 1104 36-37 0.5720792621296411 1104 38-39 0.49889560056535487 1104 40-41 0.5554314359272752 1104 42-43 0.6781476912521356 1104 44-45 0.6822076243992043 1104 46-47 0.5335630852074829 1104 48-49 0.5909368858567419 1104 50-51 0.41753732428099255 1104 52-53 0.6885764602076918 1104 54-55 0.5773196816031927 1104 56-57 0.5039373246618339 1104 58-59 0.4020319280211524 1104 60-61 0.4871735398460686 1104 62-63 0.517321710576482 1104 64-65 0.6304404872885385 1104 66-67 0.4742219790530555 1104 68-69 0.3012211134095111 1104 70-71 0.28793912161707524 1104 72-73 0.3455287140012402 1104 74-75 0.646236549880868 1104 76-77 0.6915348810282254 1104 78-79 0.8362826196798707 1104 80-81 0.569038195343655 1104 82-83 0.7554684458712657 1104 84-85 0.8777948111481599 1104 86-87 0.9194608360443546 1104 88-89 0.8705241805743995 1104 90-91 0.7770197391136726 1104 92-93 0.7627697884069953 1104 94-95 0.7696853780651765 1104 96-97 0.8898013725080247 1104 98-99 1.1034946895427105 1104 100-101 1.4598194083678706 1105 1 0.27817240753421046 1105 2 0.28145114202569843 1105 3 0.36201237873820347 1105 4 0.2007559427256851 1105 5 0.1672216331230203 1105 6 0.28509156525239376 1105 7 0.35305208220503914 1105 8 0.39815607817151744 1105 9 0.5275326638526181 1105 10-11 0.36813414180253545 1105 12-13 0.3541595254981118 1105 14-15 0.47758071265717206 1105 16-17 0.4601320406889471 1105 18-19 0.38890784129292655 1105 20-21 0.4063337100659794 1105 22-23 0.5433068034639632 1105 24-25 0.48229454078656886 1105 26-27 0.41977566310582404 1105 28-29 0.4665048023397347 1105 30-31 0.5355987090082408 1105 32-33 0.5312890876726328 1105 34-35 0.600732679655188 1105 36-37 0.634253291577032 1105 38-39 0.6181020853651162 1105 40-41 0.6554156309412207 1105 42-43 0.5271883367576038 1105 44-45 0.7279430114016563 1105 46-47 0.8266544297514002 1105 48-49 0.6898400244868625 1105 50-51 0.678604515169674 1105 52-53 0.7069382174563899 1105 54-55 0.8380490933651501 1105 56-57 0.9803793285802129 1105 58-59 0.9836854279455665 1105 60-61 0.9005529500753156 1105 62-63 0.5987985116892673 1105 64-65 0.7286034373940851 1105 66-67 0.7581347760915094 1105 68-69 0.9897159111097693 1105 70-71 0.9544889409495347 1105 72-73 0.9783098868594173 1105 74-75 1.0617730609721576 1105 76-77 1.00455257153979 1105 78-79 1.1204633788343799 1105 80-81 1.092210915751238 1105 82-83 1.2674992921688215 1105 84-85 1.4043571532512793 1105 86-87 1.3729446407707222 1105 88-89 1.4161675466587873 1105 90-91 1.1801622738627877 1105 92-93 1.2765123753032377 1105 94-95 1.1710893643484077 1105 96-97 0.997018504926416 1105 98-99 1.2226703452951924 1105 100-101 1.539431081259206 1106 1 0.14441175910575055 1106 2 0.17006983038585943 1106 3 0.10472760855909868 1106 4 0.028563422208570444 1106 5 0.03608029882857977 1106 6 0.18420929341603198 1106 7 0.1638579690340336 1106 8 0.12420760352006255 1106 9 0.2558591791882847 1106 10-11 0.27620194618285865 1106 12-13 0.18479573552642847 1106 14-15 0.29631985505983494 1106 16-17 0.1851870434390861 1106 18-19 0.15192365297460242 1106 20-21 0.019102591084283915 1106 22-23 0.18433012938272952 1106 24-25 0.09707323764219922 1106 26-27 0.18775205697502173 1106 28-29 0.28245983276452336 1106 30-31 0.16200564816708152 1106 32-33 0.25757249342707667 1106 34-35 0.1105212260131978 1106 36-37 0.2087239705565267 1106 38-39 0.18616130515778906 1106 40-41 0.19446937818303667 1106 42-43 0.3270079069440328 1106 44-45 0.2421695271828881 1106 46-47 0.03158033198710086 1106 48-49 0.1140898879701453 1106 50-51 0.20453662860703048 1106 52-53 0.02243072475502572 1106 54-55 0.24717128214786754 1106 56-57 0.22047326397004952 1106 58-59 0.24444611449474252 1106 60-61 0.18034546692888398 1106 62-63 0.23795627403283248 1106 64-65 0.13503984832354377 1106 66-67 0.08275577199278672 1106 68-69 0.17414109771563346 1106 70-71 0.029265452052314345 1106 72-73 0.04725355681519261 1106 74-75 0.2286702053343248 1106 76-77 0.14142327646117536 1106 78-79 0.24258959653140266 1106 80-81 0.17923946272313884 1106 82-83 0.3056360999993153 1106 84-85 0.30383288198821035 1106 86-87 0.19649980367737996 1106 88-89 0.2727568488961758 1106 90-91 0.43368980172432714 1106 92-93 0.5804599365426846 1106 94-95 0.4365828370418434 1106 96-97 0.6745199542563043 1106 98-99 0.604400137230332 1106 100-101 0.8813595019376663 1107 1 0.1959845243556977 1107 2 0.20002249342624978 1107 3 0.23191316764657088 1107 4 0.13766818650559287 1107 5 0.21013084341792165 1107 6 0.2179412380390673 1107 7 0.24356776725637985 1107 8 0.15202840885640967 1107 9 0.33488169938050305 1107 10-11 0.2675509149667832 1107 12-13 0.2108427754463733 1107 14-15 0.15874874745073697 1107 16-17 0.23916117008762683 1107 18-19 0.1408381175107536 1107 20-21 0.3585499079089871 1107 22-23 0.26629002803040436 1107 24-25 0.2835908511975731 1107 26-27 0.30762052222686265 1107 28-29 0.24541534758510153 1107 30-31 0.25063112201014803 1107 32-33 0.48917783834694717 1107 34-35 0.2965536581600716 1107 36-37 0.39206547746469056 1107 38-39 0.3775363396712095 1107 40-41 0.26292742830698757 1107 42-43 0.5880362523034819 1107 44-45 0.5187915830761867 1107 46-47 0.44558323559972024 1107 48-49 0.4280881067289002 1107 50-51 0.3326880650131301 1107 52-53 0.33288951149749124 1107 54-55 0.39730589693824214 1107 56-57 0.3543098203831647 1107 58-59 0.35117580739758836 1107 60-61 0.24679376458256996 1107 62-63 0.13410415294031708 1107 64-65 0.1777497671559587 1107 66-67 0.29056455120957736 1107 68-69 0.15912290154385644 1107 70-71 0.24285242536758744 1107 72-73 0.30325316009777836 1107 74-75 0.4987638675244668 1107 76-77 0.5761405854013724 1107 78-79 0.5130186743229039 1107 80-81 0.6901941711507931 1107 82-83 0.8300818169962625 1107 84-85 0.9194798702750795 1107 86-87 0.8995814905836106 1107 88-89 0.8809285049322142 1107 90-91 1.0087027847502057 1107 92-93 1.0342850948060196 1107 94-95 0.9922418913193631 1107 96-97 0.9053865625118149 1107 98-99 0.7173072335878103 1107 100-101 1.0839743232204597 1108 1 0.25144316531293853 1108 2 0.3158362993289394 1108 3 0.41901196519221884 1108 4 0.19179744942579902 1108 5 0.2585009440344592 1108 6 0.20480463642078917 1108 7 0.42197339912603127 1108 8 0.26320765535770363 1108 9 0.40252021776783664 1108 10-11 0.44555935686783243 1108 12-13 0.40225107492723566 1108 14-15 0.508962377726462 1108 16-17 0.4826253381286847 1108 18-19 0.4779254771659964 1108 20-21 0.5860137586408385 1108 22-23 0.4962945662901461 1108 24-25 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-1.2095341606991639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 63.0 5 127.0 6 268.0 7 747.0 8 1794.0 9 2911.0 10 4651.0 11 6817.0 12 9434.0 13 11951.0 14 14181.0 15 17103.0 16 20043.0 17 21717.0 18 19946.0 19 18045.0 20 18214.0 21 19184.0 22 21766.0 23 25556.0 24 30811.0 25 38455.0 26 47979.0 27 60406.0 28 74748.0 29 90428.0 30 108527.0 31 127759.0 32 151127.0 33 178609.0 34 212776.0 35 256704.0 36 312236.0 37 346785.0 38 224076.0 39 24931.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.483737710095326 21.954815444622675 15.01339238068561 23.548054464596387 2 16.28894099688476 22.584677366568908 38.142629837041966 22.983751799504372 3 18.96505557096053 26.38514838955884 31.028617150455545 23.62117888902509 4 13.769364737795073 18.424639569161492 37.470284023429144 30.33571166961429 5 12.965221171956696 38.334581592514255 35.40619728947924 13.293999946049814 6 30.72190941481964 38.62004730060478 17.18875575291027 13.469287531665303 7 26.237912045628185 33.149417390152244 22.76649035383423 17.846180210385345 8 25.204867471829484 36.30773637013477 20.533912915159597 17.95348324287615 9 25.809820869317544 15.561008640971844 20.579063722159216 38.05010676755139 10-11 24.517414032618532 26.572455529735834 28.858684484655868 20.05144595298977 12-13 24.936490885390842 24.144372362547 28.96118764027739 21.95794911178477 14-15 22.173551284810273 25.697236797608785 26.44246741487903 25.686744502701913 16-17 21.8536013753829 28.628607742161723 27.23594925300429 22.281841629451094 18-19 21.795546666307004 27.543518222474688 29.241226737456255 21.41970837376205 20-21 22.481175358939552 26.734600130588387 29.183866225448234 21.60035828502383 22-23 22.288494928590893 26.59594977502056 29.02883378416492 22.086721512223626 24-25 22.22346516973473 26.829617085556507 29.00154885805189 21.945368886656873 26-27 22.09682342816193 27.02564396523152 28.857191423595207 22.02034118301134 28-29 22.116682229903788 27.115396973618132 28.877804655325207 21.890116141152873 30-31 22.414599860001232 26.862565762030265 28.72174461709901 22.00108976086949 32-33 22.241139366694473 27.020758050229183 28.616053937006704 22.12204864606964 34-35 22.441037583798092 27.01391650888143 28.67754979781339 21.867496109507083 36-37 22.47682411597036 26.87254141451486 28.66655387561236 21.98408059390242 38-39 22.290390535544173 26.962421324737406 28.65744146548563 22.089746674232792 40-41 22.429934718331605 26.934732810876348 28.644238609625223 21.991093861166817 42-43 22.453982643358124 27.01835775009903 28.361493097272533 22.16616650927032 44-45 22.37830705567362 27.062683554507878 28.381459343383153 22.17755004643535 46-47 22.592242603989263 27.113099169799703 28.0158581863811 22.27880003982994 48-49 22.57098124377773 27.185384440371035 27.93520090880112 22.308433407050114 50-51 22.583781833440888 27.228261387876035 27.765189927741947 22.422766850941134 52-53 22.679329583219896 27.261557315697694 27.759861509850598 22.299251591231815 54-55 22.502975623690727 27.23538770392941 27.812361137561098 22.449275534818764 56-57 22.509817394576444 27.38720335817676 27.62059521469333 22.482384032553462 58-59 22.509205327454936 27.305557357507126 27.809184636422053 22.37605267861589 60-61 22.48305679894002 27.393186841840507 27.83780882286698 22.285947536352502 62-63 22.511396924239936 27.295941257699525 27.78366053086917 22.409001287191373 64-65 22.274090794486497 27.235844505458505 27.79587023459974 22.694194465455254 66-67 22.289463397027593 27.34715035576255 27.726389522818177 22.63699672439168 68-69 22.423876766255766 27.264876274494505 27.713337927340437 22.597909031909293 70-71 22.342310115664194 27.0420290643361 27.789580336650182 22.826080483349525 72-73 22.358168443381697 27.23415167017564 27.652826866344665 22.754853020097997 74-75 22.32152849852687 27.384651044349283 27.46082708654668 22.832993370577164 76-77 22.26450478686803 27.285068233253917 27.509555837512977 22.940871142365076 78-79 22.201738742003556 27.39477702991256 27.4790383184557 22.92444590962818 80-81 22.291263491592506 27.412400969425104 27.347661556471152 22.948673982511234 82-83 22.410942449873023 27.275399480661257 27.453213101045737 22.860444968419984 84-85 22.395694390589068 27.304223991653764 27.195849890763114 23.10423172699406 86-87 22.269584092652696 27.3313517643316 27.302116150673296 23.09694799234241 88-89 22.21253209922113 27.21109510929553 27.31387608644471 23.26249670503863 90-91 22.16915141834258 27.176388376198034 27.39709724301361 23.25736296244577 92-93 22.255679638286956 27.03121385815475 27.401048463152033 23.31205804040626 94-95 22.061079709709432 26.963347929580202 27.47156865720281 23.50400370350756 96-97 22.026377108474836 26.854557383916365 27.489232011767236 23.629833495841563 98-99 21.85286750991115 26.912929440223287 27.366616975379408 23.867586074486155 100-101 21.906776870781762 26.86681492071476 27.334525236641866 23.891882971861612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 253.0 1 197.0 2 183.0 3 258.5 4 237.0 5 217.0 6 383.5 7 811.0 8 1260.0 9 1397.0 10 1279.5 11 1168.5 12 1220.0 13 1350.0 14 1539.0 15 1762.5 16 2017.0 17 2313.0 18 2616.5 19 2898.5 20 3358.0 21 4000.0 22 4745.0 23 5963.0 24 7588.5 25 9810.0 26 13038.5 27 16861.5 28 21116.0 29 26603.5 30 32688.5 31 39154.5 32 46565.0 33 52058.0 34 55792.5 35 59653.0 36 65163.0 37 70967.5 38 74704.0 39 76888.0 40 78432.0 41 80219.5 42 82152.5 43 85035.5 44 87666.5 45 90587.5 46 92939.0 47 94187.5 48 95110.5 49 94111.5 50 91953.5 51 89795.5 52 87870.0 53 86045.0 54 82900.5 55 77100.0 56 69468.0 57 61056.0 58 53593.5 59 46958.0 60 39881.5 61 33203.5 62 27243.5 63 21177.5 64 15828.5 65 11950.5 66 8826.0 67 6677.0 68 5020.0 69 3657.5 70 2793.0 71 2044.0 72 1491.5 73 1169.5 74 901.0 75 671.5 76 474.0 77 289.0 78 172.5 79 110.5 80 67.5 81 40.0 82 24.0 83 22.5 84 15.5 85 6.0 86 2.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017255755687060722 2 0.002578446252089533 3 0.002657783059846134 4 0.031298370659979105 5 0.0021420938094282274 6 0.0 7 0.0 8 0.0 9 3.966840387830051E-5 10-11 0.0 12-13 0.0 14-15 1.9834201939150254E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.966840387830051E-5 24-25 3.3718143296555434E-4 26-27 0.0020429227997324764 28-29 0.0 30-31 0.006406447226345533 32-33 0.021837456335004432 34-35 0.013110407481778318 36-37 4.958550484787563E-4 38-39 5.355234523570569E-4 40-41 0.0020825912036107766 42-43 0.007596499342694548 44-45 0.007497328332998796 46-47 0.0071799811019723925 48-49 0.008508872631895458 50-51 0.1730335777171468 52-53 0.045698001267802185 54-55 0.016581392821129613 56-57 0.009698924748244474 58-59 0.019140004871279995 60-61 0.04877230256837047 62-63 0.08869855107187995 64-65 0.0933397543256411 66-67 0.0965727292417226 68-69 0.09518433510598207 70-71 0.0849300527034414 72-73 0.06513551916816944 74-75 0.22497935259578133 76-77 0.26688902129320585 78-79 0.006644457649615335 80-81 8.925390872617615E-4 82-83 0.0 84-85 1.9834201939150254E-5 86-87 0.0 88-89 1.3883941357405177E-4 90-91 0.00620810520695403 92-93 0.01773177653360033 94-95 0.0017652439725843725 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2520898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.59937200098938 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37929087349505 49.51313839692151 2 11.17243316087851 13.7642973286629 3 3.4018693315280943 6.286590433546683 4 1.5411964305575838 3.797469290100544 5 0.8801956489979224 2.7109749608137648 6 0.5650394588672034 2.088364549319916 7 0.3763639860449743 1.6228649628911684 8 0.28558649098451117 1.4073558797289683 9 0.20658932639530259 1.1453195491252253 >10 1.1141130728435202 12.05929107536737 >50 0.055167235653561776 2.3131075232643834 >100 0.020802311621441406 2.2795566443225312 >500 0.0011594332562442132 0.5131949678942703 >1k 1.2882591735946349E-4 0.29361703745138934 >5k 6.441295867973174E-5 0.20485740058939503 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5163 0.20480796922366556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4322 0.1714468415620148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3078 0.12209934713740898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.966840387830051E-5 0.0 10-11 0.0 0.0 0.0 3.966840387830051E-5 0.0 12-13 0.0 0.0 0.0 3.966840387830051E-5 2.3801042326980303E-4 14-15 0.0 0.0 0.0 3.966840387830051E-5 4.5618664460045583E-4 16-17 0.0 0.0 0.0 3.966840387830051E-5 5.156892504179066E-4 18-19 0.0 0.0 0.0 3.966840387830051E-5 5.156892504179066E-4 20-21 0.0 0.0 0.0 3.966840387830051E-5 5.553576542962072E-4 22-23 0.0 1.9834201939150254E-5 0.0 9.917100969575127E-5 5.950260581745077E-4 24-25 0.0 3.966840387830051E-5 0.0 1.5867361551320203E-4 6.346944620528081E-4 26-27 0.0 3.966840387830051E-5 0.0 4.5618664460045583E-4 6.743628659311087E-4 28-29 0.0 3.966840387830051E-5 0.0 0.001864414982280124 6.743628659311087E-4 30-31 0.0 3.966840387830051E-5 0.0 0.006426281428284683 6.743628659311087E-4 32-33 0.0 3.966840387830051E-5 0.0 0.019774699333332806 6.743628659311087E-4 34-35 0.0 3.966840387830051E-5 0.0 0.03826017554062085 7.140312698094091E-4 36-37 0.0 3.966840387830051E-5 0.0 0.06711893936208446 7.140312698094091E-4 38-39 0.0 3.966840387830051E-5 0.0 0.11295578004346071 7.536996736877097E-4 40-41 0.0 3.966840387830051E-5 0.0 0.16293796893011936 7.536996736877097E-4 42-43 0.0 3.966840387830051E-5 0.0 0.21518125683784112 7.933680775660103E-4 44-45 0.0 3.966840387830051E-5 0.0 0.28031677600601057 8.52870683383461E-4 46-47 0.0 3.966840387830051E-5 0.0 0.3584238632423843 8.925390872617615E-4 48-49 0.0 3.966840387830051E-5 0.0 0.436134266439975 9.123732892009118E-4 50-51 0.0 3.966840387830051E-5 0.0 0.5217981846151649 9.520416930792122E-4 52-53 0.0 3.966840387830051E-5 0.0 0.6082951392717992 0.001071046904714114 54-55 0.0 3.966840387830051E-5 0.0 0.7077239935927594 0.0011107153085924144 56-57 0.0 3.966840387830051E-5 0.0 0.8257573293326426 0.0011305495105315647 58-59 0.0 3.966840387830051E-5 0.0 0.9528945637625957 0.0011503837124707149 60-61 0.0 3.966840387830051E-5 0.0 1.0923488375967612 0.0011900521163490153 62-63 0.0 3.966840387830051E-5 0.0 1.23289399253758 0.0011900521163490153 64-65 0.0 3.966840387830051E-5 0.0 1.3863710471427244 0.0012297205202273158 66-67 0.0 3.966840387830051E-5 0.0 1.55920628284048 0.0012892231260447666 68-69 0.0 3.966840387830051E-5 0.0 1.7547715139605014 0.001309057327983917 70-71 0.0 3.966840387830051E-5 0.0 1.9686833818742369 0.0013685599338013675 72-73 0.0 3.966840387830051E-5 0.0 2.1951304654135155 0.0015669019531928702 74-75 0.0 3.966840387830051E-5 0.0 2.4320698417786044 0.001626404559010321 76-77 0.0 3.966840387830051E-5 0.0 2.698363837013636 0.0016660729628886215 78-79 0.0 3.966840387830051E-5 0.0 2.997443768054082 0.0017255755687060723 80-81 0.0 3.966840387830051E-5 0.0 3.3135612785602593 0.0019040833861584242 82-83 0.0 3.966840387830051E-5 0.0 3.656276453866836 0.0022015964152456782 84-85 0.0 3.966840387830051E-5 0.0 4.013926783233593 0.002221430617184829 86-87 0.0 3.966840387830051E-5 0.0 4.396548372841741 0.002241264819123979 88-89 0.0 3.966840387830051E-5 0.0 4.821139133753131 0.002261099021063129 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 55.04852 1 GTATCAA 5960 0.0 41.916115 1 TCAACGC 7875 0.0 30.931164 4 ATCAACG 7835 0.0 30.907272 3 CAACGCA 7890 0.0 30.681473 5 TATCAAC 8070 0.0 30.184359 2 AACGCAG 8380 0.0 29.283361 6 ACGCAGA 9540 0.0 25.125656 7 GTACATG 7490 0.0 24.79342 1 CGCAGAG 9930 0.0 24.04325 8 TACATGG 7600 0.0 23.67902 2 ACATGGG 7640 0.0 22.870935 3 GTGGTAT 1525 0.0 21.489225 1 GCAGAGT 10885 0.0 21.192503 9 CATGGGG 5365 0.0 21.0638 4 AGAGTAC 10125 0.0 20.275217 10-11 GAGTACA 5735 0.0 19.875559 1 GAGTACT 7225 0.0 19.084585 12-13 AGTACTT 7780 0.0 19.065353 12-13 GGACCGT 385 1.2732926E-11 18.492897 6 >>END_MODULE