FastQCFastQC Report
Sat 18 Jun 2016
SRR1930964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956962
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72870.18415643112064256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.053.7574421
GTATCAA56000.042.9189831
TCAACGC70500.034.7025224
ATCAACG69450.034.5169753
CAACGCA71450.033.9625745
TATCAAC70300.033.8933372
AACGCAG79350.031.495796
GTACATG82400.028.0675531
ACGCAGA92200.026.723547
TACATGG85000.026.253872
CGCAGAG95850.025.8030838
ACATGGG89250.024.4330483
GCAGAGT107550.022.0742869
CATGGGG69550.020.772974
GTGTAGC15100.020.688731
AGAGTAC94100.018.82658410-11
GAGTACT62350.018.71894312-13
CAGAGTA102800.018.39507910-11
GTATTAA22550.017.8756791
GAGTACA61900.017.6639631
Produced by FastQC (version 0.11.5)