FastQCFastQC Report
Sat 18 Jun 2016
SRR1930964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956962
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.1448838780862692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46580.11771657145052189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.054.0262571
GTATCAA76600.044.132291
CAACGCA98100.033.8125085
TCAACGC98450.033.7989544
ATCAACG98750.033.649053
TATCAAC99600.033.3159142
AACGCAG105250.031.7405386
ACGCAGA119900.027.4665437
CGCAGAG123550.026.5782958
GTACATG111150.023.28051
TACATGG113750.022.4942362
GCAGAGT144300.022.1315829
ACATGGG111550.021.8303013
AGAGTAC128950.019.89015210-11
CATGGGG82750.018.98724
CAGAGTA135600.018.87971910-11
GAGTACT85750.018.78751812-13
GAGTACA85350.018.6357361
AGTACTT92950.017.35774212-13
GTACTTT93600.017.03441214-15
Produced by FastQC (version 0.11.5)