##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930964_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3956962 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266195884620576 31.0 31.0 34.0 30.0 34.0 2 31.392668668539148 31.0 31.0 34.0 30.0 34.0 3 31.477796602545084 31.0 31.0 34.0 30.0 34.0 4 35.34370130418235 37.0 35.0 37.0 33.0 37.0 5 35.13364242567909 37.0 35.0 37.0 32.0 37.0 6 35.17069105035631 37.0 35.0 37.0 32.0 37.0 7 35.04727414617578 37.0 35.0 37.0 32.0 37.0 8 35.022564280374695 37.0 35.0 37.0 32.0 37.0 9 36.53388584474655 38.0 35.0 39.0 32.0 39.0 10-11 36.45891772526499 38.0 35.0 39.0 32.0 39.0 12-13 36.35811981515112 38.0 35.0 39.0 32.0 39.0 14-15 37.38592700662781 39.0 36.0 41.0 32.0 41.0 16-17 37.35048074760384 39.0 36.0 41.0 32.0 41.0 18-19 37.3296925772853 39.0 36.0 40.0 32.0 41.0 20-21 37.28717321520904 39.0 36.0 40.0 31.5 41.0 22-23 37.15597951155458 39.0 36.0 40.0 31.0 41.0 24-25 37.035687100356284 39.0 36.0 40.0 31.0 41.0 26-27 36.76484232095228 39.0 35.5 40.0 30.5 41.0 28-29 36.582554116011224 38.0 35.0 40.0 30.0 41.0 30-31 36.340471174603145 38.0 35.0 40.0 30.0 41.0 32-33 36.10942548854399 38.0 35.0 40.0 30.0 41.0 34-35 36.32244345535793 38.0 35.0 40.0 30.0 41.0 36-37 36.3774310948652 38.0 35.0 40.0 30.0 41.0 38-39 36.34083597466946 38.0 35.0 40.0 30.0 41.0 40-41 36.24172306936483 38.0 35.0 40.0 30.0 41.0 42-43 36.09869604509723 38.0 35.0 40.0 29.5 41.0 44-45 35.91387041371638 38.0 35.0 40.0 29.0 41.0 46-47 35.696735904969515 38.0 34.0 40.0 28.0 41.0 48-49 35.49874802942257 38.0 34.0 40.0 27.5 41.0 50-51 35.22010117863148 38.0 34.0 40.0 27.0 41.0 52-53 34.99650173542227 38.0 33.5 40.0 26.5 41.0 54-55 34.81453663189083 38.0 33.0 40.0 26.0 41.0 56-57 34.55065818170607 37.0 33.0 40.0 26.0 41.0 58-59 34.29533187329067 37.0 33.0 40.0 26.0 41.0 60-61 33.974666170663255 36.5 32.5 39.5 25.5 41.0 62-63 33.661285728799015 36.0 32.0 39.0 24.5 41.0 64-65 33.34192873219404 36.0 32.0 39.0 23.5 40.5 66-67 32.99767725846243 35.0 31.0 39.0 23.0 40.0 68-69 32.61479829727958 35.0 31.0 38.0 22.5 40.0 70-71 32.20996676237982 35.0 31.0 38.0 22.0 40.0 72-73 31.791702573843267 34.5 30.5 37.0 21.5 39.5 74-75 31.264514291519603 34.0 30.0 36.5 20.0 39.0 76-77 29.901318486252837 32.5 28.5 35.0 18.5 37.5 78-79 30.667771638949276 34.0 29.5 36.0 20.0 38.0 80-81 30.671535384974636 34.0 30.0 35.0 20.0 37.0 82-83 30.407666790836 34.0 30.0 35.0 18.5 37.0 84-85 30.09778625622384 34.0 30.0 35.0 18.0 36.5 86-87 29.768796743562362 34.0 29.0 35.0 16.5 36.0 88-89 29.43052473084149 34.0 29.0 35.0 11.5 36.0 90-91 29.097480086996036 34.0 29.0 35.0 7.0 35.0 92-93 28.74764781668361 33.5 29.0 35.0 2.0 35.0 94-95 28.332425102894593 33.0 29.0 35.0 2.0 35.0 96-97 27.86974337888511 33.0 27.0 35.0 2.0 35.0 98-99 27.379080845355602 33.0 27.0 35.0 2.0 35.0 100-101 26.161175922336376 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4942364893392508 1101 2 0.6172069155763644 1101 3 0.5516884993539861 1101 4 0.3714728998353678 1101 5 0.49594422562201146 1101 6 0.4538197403552573 1101 7 0.48694201121507064 1101 8 0.3966742421241989 1101 9 0.6025247480664788 1101 10-11 0.6666567524372198 1101 12-13 0.6522054011926315 1101 14-15 0.744039947067705 1101 16-17 0.8121572711955523 1101 18-19 0.7583485961034526 1101 20-21 0.7588943018346939 1101 22-23 0.7901897770041657 1101 24-25 0.6943880185508036 1101 26-27 0.7733712672919708 1101 28-29 0.8075611111842633 1101 30-31 0.7742087633398924 1101 32-33 0.7056214970568675 1101 34-35 0.7361517157476882 1101 36-37 0.8607610855918253 1101 38-39 0.9976522440483535 1101 40-41 0.8529907146430773 1101 42-43 0.8300745443667665 1101 44-45 0.8595057606533913 1101 46-47 0.9087232771692086 1101 48-49 0.8860298614080122 1101 50-51 0.8459959460871787 1101 52-53 0.710696856774895 1101 54-55 0.7245974477428092 1101 56-57 1.0000743938408974 1101 58-59 0.8124238864718052 1101 60-61 0.7329318928601367 1101 62-63 0.7351817874509408 1101 64-65 0.753192328111048 1101 66-67 0.8711551264197013 1101 68-69 0.7767230837350709 1101 70-71 0.7887331612522033 1101 72-73 0.8009394127622222 1101 74-75 0.7636180010613138 1101 76-77 0.9257604643219182 1101 78-79 0.9968156645744592 1101 80-81 0.8056078926909827 1101 82-83 1.0096648710313723 1101 84-85 0.9869513457314305 1101 86-87 0.8918663956360717 1101 88-89 0.7698585109681453 1101 90-91 0.8406998392202851 1101 92-93 0.8847912367943458 1101 94-95 0.9613673420433457 1101 96-97 1.0034469551664351 1101 98-99 1.1201237080189053 1101 100-101 1.2502341050175119 1102 1 0.4828127488041609 1102 2 0.5216723491983792 1102 3 0.5458286420931913 1102 4 0.2651878614147307 1102 5 0.423435630363862 1102 6 0.48863989119004714 1102 7 0.502438129905542 1102 8 0.5160584250378832 1102 9 0.6526661753302676 1102 10-11 0.61729397071948 1102 12-13 0.650085281540143 1102 14-15 0.7145766360810555 1102 16-17 0.7237144767533223 1102 18-19 0.792372302701537 1102 20-21 0.8028776022603594 1102 22-23 0.8178423699589246 1102 24-25 0.8135590259805952 1102 26-27 0.8700329687738417 1102 28-29 0.9095294010873332 1102 30-31 0.8963582212722727 1102 32-33 0.9713846275534692 1102 34-35 0.8863358095547156 1102 36-37 0.9615604480590818 1102 38-39 1.0203812348952965 1102 40-41 1.0844465544563704 1102 42-43 0.9531000689470162 1102 44-45 1.0605196759590427 1102 46-47 1.1405496819481655 1102 48-49 1.1363652377962339 1102 50-51 1.166887753570002 1102 52-53 1.2176885032141556 1102 54-55 1.1204206107701324 1102 56-57 1.1314347999680194 1102 58-59 1.1641218626914949 1102 60-61 1.090938404287833 1102 62-63 0.9767603967203726 1102 64-65 1.1511149819197186 1102 66-67 1.1923115557784527 1102 68-69 1.0624817853136719 1102 70-71 1.1044424994516078 1102 72-73 1.0408090332652016 1102 74-75 1.2758195695347645 1102 76-77 1.3823155271185925 1102 78-79 1.4039719080218127 1102 80-81 1.363488228383094 1102 82-83 1.451658623753847 1102 84-85 1.6019406985924256 1102 86-87 1.5323520898284002 1102 88-89 1.5416702717643105 1102 90-91 1.5156164086440462 1102 92-93 1.6262569533086229 1102 94-95 1.530828933538114 1102 96-97 1.4622263197041256 1102 98-99 1.5860078004297051 1102 100-101 1.881527372974091 1103 1 0.3996087201753653 1103 2 0.47515190208637037 1103 3 0.3877303904233287 1103 4 0.3289330412453495 1103 5 0.281871452971842 1103 6 0.4514716005868138 1103 7 0.44624026580715537 1103 8 0.358270015177375 1103 9 0.5332006206351139 1103 10-11 0.5858264879565738 1103 12-13 0.5315169464919904 1103 14-15 0.7206477978850501 1103 16-17 0.5992522945859164 1103 18-19 0.5786061730613739 1103 20-21 0.6888206961164869 1103 22-23 0.7425429173950633 1103 24-25 0.6913124819649497 1103 26-27 0.7259425160132125 1103 28-29 0.6370886919852197 1103 30-31 0.6767660988901483 1103 32-33 0.8231004194024223 1103 34-35 0.6863415487256717 1103 36-37 0.6903091178930154 1103 38-39 0.7866586984803163 1103 40-41 0.8246966330181564 1103 42-43 0.8420144992541481 1103 44-45 0.7492417277873074 1103 46-47 0.7644561802070768 1103 48-49 0.7314349105974074 1103 50-51 0.7485938463424162 1103 52-53 0.7691867327594579 1103 54-55 0.9338348399003564 1103 56-57 0.9757739827688141 1103 58-59 0.9135482515219238 1103 60-61 0.8637667827627808 1103 62-63 0.7536070514567399 1103 64-65 0.733381959341898 1103 66-67 0.7136867859571439 1103 68-69 0.8184354715501385 1103 70-71 0.7054622485629523 1103 72-73 0.6969574964400387 1103 74-75 0.8396219575193662 1103 76-77 0.9520871423782822 1103 78-79 1.0173983619532727 1103 80-81 0.9901202821790704 1103 82-83 0.9956121685301476 1103 84-85 1.041445859685691 1103 86-87 1.09954695193656 1103 88-89 1.0284036090787616 1103 90-91 1.0261453399394398 1103 92-93 1.1287476800810126 1103 94-95 1.155932458881999 1103 96-97 1.0047712590035225 1103 98-99 1.1820600462199202 1103 100-101 1.3238009192384226 1104 1 0.364126389009769 1104 2 0.45526194679033694 1104 3 0.41068570341942845 1104 4 0.2688705961122011 1104 5 0.36494852502453057 1104 6 0.39189238427983497 1104 7 0.3902455660098241 1104 8 0.4963777443411672 1104 9 0.5198755445224492 1104 10-11 0.48011320239165656 1104 12-13 0.492850511976215 1104 14-15 0.638759772677048 1104 16-17 0.5669289746837194 1104 18-19 0.6556885924380254 1104 20-21 0.5562726901245441 1104 22-23 0.5970842964844323 1104 24-25 0.584667461248074 1104 26-27 0.7548111091081537 1104 28-29 0.5997283712682986 1104 30-31 0.7001684456717427 1104 32-33 0.7951230076994733 1104 34-35 0.7287071517391652 1104 36-37 0.7441588565819046 1104 38-39 0.6612541033063835 1104 40-41 0.6043172229955545 1104 42-43 0.6888646825323974 1104 44-45 0.8011605538263353 1104 46-47 0.8400028578498677 1104 48-49 0.7859356888055089 1104 50-51 0.7886865833966681 1104 52-53 0.8274989164872508 1104 54-55 0.7866773786917847 1104 56-57 0.8293829493376705 1104 58-59 0.8054361389001556 1104 60-61 0.8451120237245675 1104 62-63 0.7005172908187589 1104 64-65 0.730810108799318 1104 66-67 0.7653289216730599 1104 68-69 0.7129486332331751 1104 70-71 0.6929532248359607 1104 72-73 0.6271439777661136 1104 74-75 0.836332037422931 1104 76-77 0.9286276610328912 1104 78-79 0.9683471609022796 1104 80-81 0.8571584923616982 1104 82-83 0.8987871362402835 1104 84-85 0.9599430804720193 1104 86-87 0.9493461033292547 1104 88-89 0.907633863962527 1104 90-91 0.8054779599349828 1104 92-93 0.8199542112802511 1104 94-95 0.8154373766954031 1104 96-97 0.7880167130948941 1104 98-99 0.9919193102419186 1104 100-101 1.257626872278287 1105 1 0.1796103776474567 1105 2 0.3495096316509638 1105 3 0.3559123413056504 1105 4 0.16544790481467686 1105 5 0.18004757444947472 1105 6 0.23461586281396762 1105 7 0.2614467246745349 1105 8 0.315785285580759 1105 9 0.38991545444528697 1105 10-11 0.31726998134269735 1105 12-13 0.24609436644833238 1105 14-15 0.44927182294301105 1105 16-17 0.4001324499362582 1105 18-19 0.4970258205095206 1105 20-21 0.4427053963306449 1105 22-23 0.5490336393183455 1105 24-25 0.4893061873379807 1105 26-27 0.5445073570748278 1105 28-29 0.498430203750452 1105 30-31 0.5872992163126227 1105 32-33 0.5657692837891943 1105 34-35 0.5574776846308467 1105 36-37 0.6349688558240771 1105 38-39 0.48489754020868503 1105 40-41 0.47137973471348715 1105 42-43 0.46104677569788777 1105 44-45 0.4978427648374577 1105 46-47 0.5376614957954189 1105 48-49 0.5841084790029782 1105 50-51 0.5170355777227016 1105 52-53 0.4207547861149905 1105 54-55 0.5160427481140744 1105 56-57 0.566725291700898 1105 58-59 0.5708952084596675 1105 60-61 0.5083855783079514 1105 62-63 0.4341402536363148 1105 64-65 0.5192696439475597 1105 66-67 0.48603240561627103 1105 68-69 0.5567992640723034 1105 70-71 0.4545790089960775 1105 72-73 0.42604568431450573 1105 74-75 0.5510046235632231 1105 76-77 0.6051279679877304 1105 78-79 0.6933543278168806 1105 80-81 0.49069427606525906 1105 82-83 0.5966050743612499 1105 84-85 0.652652230970741 1105 86-87 0.6238942516696682 1105 88-89 0.6299736871871318 1105 90-91 0.6065764098188389 1105 92-93 0.6768819409708868 1105 94-95 0.5433272423652902 1105 96-97 0.4826999227231532 1105 98-99 0.5837073239161832 1105 100-101 0.914826626065512 1106 1 0.07325788880752171 1106 2 0.10898771011073194 1106 3 0.14585311764779263 1106 4 -0.0175904114206773 1106 5 0.08362760415212733 1106 6 0.09181019537965085 1106 7 0.08995723741872297 1106 8 0.11770083624112715 1106 9 0.13623038017390598 1106 10-11 0.04048336088519733 1106 12-13 -0.026068612816800396 1106 14-15 0.10246779798661265 1106 16-17 0.030580253354763443 1106 18-19 0.1022079530107618 1106 20-21 0.08251568150286204 1106 22-23 0.20193813924012005 1106 24-25 0.1512598119716131 1106 26-27 0.16867240998536914 1106 28-29 0.10357473113617033 1106 30-31 0.11561481819541797 1106 32-33 0.1246275618302306 1106 34-35 0.11612940415810158 1106 36-37 0.19569984023688392 1106 38-39 0.28918070894230397 1106 40-41 0.18680638617690448 1106 42-43 0.27573540761186166 1106 44-45 0.13935895995584247 1106 46-47 0.26654683362615117 1106 48-49 0.23515531459920425 1106 50-51 0.1710947107877061 1106 52-53 0.11798723125239263 1106 54-55 0.1223975074515522 1106 56-57 0.22790073168358305 1106 58-59 0.28002281859525624 1106 60-61 0.04579463636707004 1106 62-63 0.19450839551335264 1106 64-65 0.08333454180582578 1106 66-67 0.062172235115532715 1106 68-69 0.11317801689254026 1106 70-71 0.03857257463309338 1106 72-73 0.09913047990501411 1106 74-75 0.2533656969273146 1106 76-77 0.31099250420114544 1106 78-79 0.2838430773880454 1106 80-81 0.1962184646972851 1106 82-83 0.23249761602658126 1106 84-85 0.1663859313527034 1106 86-87 0.21944132918740777 1106 88-89 0.3043320048483551 1106 90-91 0.20013893057970122 1106 92-93 0.2307571864400515 1106 94-95 0.25453340785594847 1106 96-97 0.19934753423920526 1106 98-99 0.031197811099112016 1106 100-101 0.31135080084749944 1107 1 0.1434694611414855 1107 2 0.1860346834868416 1107 3 0.20411290790100978 1107 4 0.03590060294450126 1107 5 0.11161140258448654 1107 6 0.14799819224869992 1107 7 0.1715180762391384 1107 8 0.1662726958953371 1107 9 0.22011108742118068 1107 10-11 0.17042091699591566 1107 12-13 0.18808366824822542 1107 14-15 0.18731913218186946 1107 16-17 0.20155914113498596 1107 18-19 0.3187016923669148 1107 20-21 0.22983516231247592 1107 22-23 0.22336895539533685 1107 24-25 0.29871741789740014 1107 26-27 0.25146912265761756 1107 28-29 0.3569284539706601 1107 30-31 0.378003107319536 1107 32-33 0.4387935970558843 1107 34-35 0.23695770471902478 1107 36-37 0.2911541318267581 1107 38-39 0.3647653232881396 1107 40-41 0.281866141752964 1107 42-43 0.49030698217983115 1107 44-45 0.49655409481746915 1107 46-47 0.541312283508077 1107 48-49 0.5022264369470477 1107 50-51 0.46561588677676724 1107 52-53 0.30736293406916815 1107 54-55 0.4243929444045662 1107 56-57 0.30997339199666385 1107 58-59 0.4131555079004343 1107 60-61 0.46368054685499516 1107 62-63 0.2492575404630344 1107 64-65 0.15060439093518596 1107 66-67 0.16245640441007225 1107 68-69 0.2940225556988665 1107 70-71 0.25731457742227093 1107 72-73 0.24215499482077618 1107 74-75 0.35215399705476713 1107 76-77 0.4833779841421588 1107 78-79 0.6012439244776182 1107 80-81 0.6560335587632444 1107 82-83 0.6330301104394742 1107 84-85 0.6165766973927624 1107 86-87 0.46731497763272856 1107 88-89 0.527702657804209 1107 90-91 0.6473385417954312 1107 92-93 0.5765330336338259 1107 94-95 0.6671567264098144 1107 96-97 0.6621835421315936 1107 98-99 0.5443206140803447 1107 100-101 0.9750308974100435 1108 1 0.36665376712909037 1108 2 0.33814492546752106 1108 3 0.38412290793456805 1108 4 0.14662798793573018 1108 5 0.2463625259103992 1108 6 0.18313706855365552 1108 7 0.38446912641044406 1108 8 0.4257863184242723 1108 9 0.388525914316574 1108 10-11 0.45171741463516923 1108 12-13 0.4847709054537148 1108 14-15 0.4802283365751805 1108 16-17 0.6284852382024226 1108 18-19 0.6213623556577375 1108 20-21 0.5665089430969701 1108 22-23 0.5982149280328386 1108 24-25 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-1.226753180856953 2316 54-55 -1.4541374135223677 2316 56-57 -1.4101158781605605 2316 58-59 -1.352423110273996 2316 60-61 -1.1400194950619564 2316 62-63 -1.3275157904010797 2316 64-65 -1.1492545251781507 2316 66-67 -0.7967244527865418 2316 68-69 -0.8840839531768765 2316 70-71 -0.9275804858364793 2316 72-73 -0.849585374984283 2316 74-75 -0.5775665134757482 2316 76-77 -0.8666016547746906 2316 78-79 -0.7907296270229764 2316 80-81 -0.7873638248624637 2316 82-83 -0.8851271966006813 2316 84-85 -0.887820101785227 2316 86-87 -0.8631786566662853 2316 88-89 -0.8528865093030333 2316 90-91 -0.6381733278697403 2316 92-93 -0.8721236824868761 2316 94-95 -0.6769651931673941 2316 96-97 -0.7678173439986757 2316 98-99 -0.8703869579525865 2316 100-101 -0.897026527813999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 32.0 4 48.0 5 121.0 6 263.0 7 694.0 8 1733.0 9 2996.0 10 4903.0 11 7349.0 12 10041.0 13 12875.0 14 15329.0 15 18498.0 16 22054.0 17 24976.0 18 23281.0 19 20973.0 20 21524.0 21 23257.0 22 26074.0 23 30545.0 24 37021.0 25 47147.0 26 60334.0 27 76942.0 28 97577.0 29 120946.0 30 147847.0 31 178471.0 32 214160.0 33 260115.0 34 320326.0 35 397849.0 36 512775.0 37 639577.0 38 502266.0 39 76021.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78228741621048 20.678212615006398 14.305773252214946 24.233726716568178 2 15.697349400026283 21.30164474681817 39.21358963213069 23.787416221024856 3 18.553337125957498 25.747326819151723 30.40421774852071 25.295118306370068 4 13.249392386259814 17.525693706046912 37.785712443858614 31.43920146383466 5 13.268821307670049 37.94724890917982 35.31598719699966 13.46794258615047 6 31.389139291491624 37.76941445720592 17.358295924574442 13.48315032672801 7 27.064904843665417 32.50273821178975 22.595086836820773 17.837270107724056 8 25.392889797779205 36.133250711025276 20.355995331772203 18.117864159423316 9 25.874319743277795 15.697295046047955 20.515739094790398 37.91264611588385 10-11 24.7109145854825 26.24138164581818 28.742201719399883 20.305502049299438 12-13 25.16914491470982 23.740359396931282 28.74342740718763 22.34706828117126 14-15 22.384205357544502 25.361919068214455 26.021642360982995 26.23223321325805 16-17 22.25689809505373 28.325417327737796 26.82108142559873 22.596603151609745 18-19 22.240471351506535 27.456670041309465 28.46777401450911 21.835084592674885 20-21 22.887002705611025 26.654173075202642 28.37505136516348 22.08377285402286 22-23 22.72024483441834 26.492206138045365 28.237738061542196 22.549810965994098 24-25 22.621120377948472 26.707243460637464 28.262522961231547 22.409113200182514 26-27 22.54094171019533 26.867183463315943 28.086984288487216 22.50489053800151 28-29 22.540492926823937 26.9840216843425 28.08472875554735 22.390756633286212 30-31 22.7485025990165 26.77861464471936 28.033135786006934 22.4397469702572 32-33 22.651079639060757 26.846028284401115 27.87734284109156 22.625549235446567 34-35 22.795316180323216 26.850526067825303 28.00586084067223 22.348296911179247 36-37 22.908624616196043 26.689264962747057 27.929871487670194 22.472238933386706 38-39 22.704811009633847 26.799382198517563 27.98765054109438 22.50815625075421 40-41 22.78462746097045 26.64881419019155 28.04386745698751 22.522690891850488 42-43 22.75581222692248 26.769447542262842 27.753844015278123 22.720896215536552 44-45 22.73610024680063 26.8717988897131 27.786351280823222 22.605749582663044 46-47 22.893332846390457 26.79668968145722 27.52702817288882 22.782949299263507 48-49 22.83561813485384 26.914304778369196 27.45827204302706 22.791805043749903 50-51 22.876513303703433 26.955625287860986 27.33250636997267 22.83535503846291 52-53 22.917585335018963 26.93504424778761 27.34734513274336 22.80002528445006 54-55 22.75416280306254 26.933907377386994 27.424408693213547 22.887521126336914 56-57 22.828073892125502 27.022711570922304 27.26348943012621 22.885725106825983 58-59 22.790718542629957 26.94037853553976 27.427769425839525 22.841133495990753 60-61 22.821468382417176 27.100873775569408 27.3629571398247 22.71470070218872 62-63 22.784882973076172 27.000637336376627 27.332484816305087 22.88199487424211 64-65 22.5940682199107 26.949587977016414 27.350435890959147 23.10590791211374 66-67 22.641154542196816 26.993848346233136 27.280553637454798 23.084443474115254 68-69 22.710831997079616 26.941126455243225 27.31282782065694 23.03521372702022 70-71 22.637762162761042 26.773264071664375 27.336841442262283 23.252132323312296 72-73 22.713544532155584 26.92350635886434 27.20180506597471 23.161144043005365 74-75 22.672157957450604 27.037551729436128 27.157163878524322 23.13312643458895 76-77 22.595692796060547 26.91039929455668 27.11503412713872 23.378873782244053 78-79 22.538108835305433 26.95998224780587 27.151354135548516 23.35055478134018 80-81 22.590577933768756 27.04815659840826 27.090740181009288 23.270525286813697 82-83 22.641296530014692 26.89346018485899 27.22111053884268 23.24413274628364 84-85 22.64802172070666 26.907792759671782 27.000098434114157 23.444087085507405 86-87 22.4678806619826 26.984262168805262 27.117975861279437 23.4298813079327 88-89 22.539699708563855 26.813237246004253 27.051956099587667 23.59510694584423 90-91 22.49545739504747 26.828923709383982 27.072270099455213 23.603348796113334 92-93 22.539274906334857 26.716819410312425 27.17825414107687 23.565651542275855 94-95 22.358909013805516 26.60728848893464 27.251303230789176 23.782499266470673 96-97 22.33773030926251 26.494467220054176 27.25340804384778 23.914394426835536 98-99 22.151640576785926 26.584485774692805 27.145218983654633 24.118654664866632 100-101 22.226609707144014 26.507697066587955 27.118531843368725 24.147161382899306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251.0 1 204.0 2 215.5 3 281.0 4 246.5 5 235.0 6 392.5 7 801.0 8 1275.5 9 1476.0 10 1328.5 11 1171.0 12 1231.0 13 1392.0 14 1604.5 15 1811.0 16 2085.0 17 2465.0 18 2735.5 19 3024.5 20 3545.0 21 4216.0 22 5122.0 23 6410.0 24 8275.5 25 10952.0 26 14804.5 27 19684.0 28 25555.5 29 33679.0 30 43290.0 31 53553.5 32 65559.5 33 74739.5 34 81428.0 35 88540.0 36 97304.5 37 108027.5 38 115190.5 39 119182.5 40 122365.5 41 125899.5 42 129676.5 43 134255.5 44 138413.0 45 143363.5 46 148499.0 47 151920.5 48 154417.0 49 153544.0 50 149576.5 51 146328.5 52 145323.0 53 143257.0 54 138261.5 55 129060.0 56 117441.5 57 104636.0 58 92571.0 59 81295.5 60 68685.0 61 56466.5 62 45533.0 63 34969.5 64 25794.5 65 19061.0 66 13982.5 67 10719.0 68 8167.0 69 5881.0 70 4299.0 71 3130.0 72 2215.0 73 1581.5 74 1120.5 75 762.0 76 542.5 77 344.0 78 188.0 79 105.0 80 61.0 81 38.0 82 22.5 83 11.5 84 6.5 85 5.5 86 3.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019434101211990414 2 0.003083173404242952 3 0.00401823419077565 4 0.03346001300998089 5 0.0019459373125139945 6 2.5271913149532394E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.054382629906479E-5 24-25 5.180742195654141E-4 26-27 0.002186020487434552 28-29 1.3899552232242817E-4 30-31 0.006899232289822344 32-33 0.022605726312256727 34-35 0.013469929708700767 36-37 6.697056984626084E-4 38-39 6.949776116121408E-4 40-41 0.0022239283571588507 42-43 0.00820073581702326 44-45 0.008263915599897092 46-47 0.007670025640883082 48-49 0.009653870823121375 50-51 0.19138419828140882 52-53 0.04958349359938255 54-55 0.017778790900696038 56-57 0.010374120347883048 58-59 0.020078534997303485 60-61 0.053412188441536716 62-63 0.09579318679330254 64-65 0.10024104350762023 66-67 0.1041834619589473 68-69 0.10254078760422768 70-71 0.09211612343004558 72-73 0.06968730050983557 74-75 0.24312844045507642 76-77 0.29420297693028136 78-79 0.006772872724074681 80-81 9.603326996822309E-4 82-83 0.0 84-85 1.2635956574766197E-5 86-87 0.0 88-89 1.5163147889719436E-4 90-91 0.006482245722855059 92-93 0.018056781945340897 94-95 0.0016426743547196055 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3956962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.378935859088784 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67714109783381 40.60863465899227 2 13.839406802506671 15.051444296826947 3 4.696118205548086 7.661097320585954 4 2.1233209630031866 4.618557378216356 5 1.1876349845875558 3.2291163325448298 6 0.7354253402499787 2.3994988443961285 7 0.5160814792621902 1.964477316120361 8 0.3703116194557548 1.610972144180783 9 0.26654737960978053 1.3045106573287601 >10 1.4676911849968506 14.183539725249242 >50 0.08621184737231043 3.1966952990086424 >100 0.03229736691989845 3.1070372072175645 >500 0.0013936374484371807 0.5534290882228365 >1k 3.7163662723274193E-4 0.36616552068561203 >5k 4.6454578404090444E-5 0.1448242104236326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5733 0.1448838780862692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4658 0.11771657145052189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5271913149532394E-5 2 0.0 0.0 0.0 0.0 2.5271913149532394E-5 3 0.0 0.0 0.0 0.0 2.5271913149532394E-5 4 0.0 0.0 0.0 0.0 2.5271913149532394E-5 5 0.0 0.0 0.0 0.0 2.5271913149532394E-5 6 0.0 0.0 0.0 0.0 5.054382629906479E-5 7 0.0 0.0 0.0 0.0 5.054382629906479E-5 8 0.0 0.0 0.0 0.0 7.581573944859718E-5 9 0.0 0.0 0.0 0.0 7.581573944859718E-5 10-11 0.0 0.0 0.0 0.0 1.0108765259812958E-4 12-13 0.0 0.0 0.0 0.0 2.2744721834579157E-4 14-15 0.0 0.0 0.0 0.0 2.7799104464485634E-4 16-17 0.0 0.0 0.0 0.0 3.285348709439211E-4 18-19 0.0 0.0 0.0 1.2635956574766197E-5 3.538067840934535E-4 20-21 0.0 0.0 0.0 6.317978287383099E-5 4.422584801168169E-4 22-23 0.0 0.0 0.0 1.3899552232242817E-4 4.8016634984111546E-4 24-25 0.0 1.2635956574766197E-5 0.0 3.032629577943887E-4 4.8016634984111546E-4 26-27 0.0 2.5271913149532394E-5 0.0 5.433461327149465E-4 5.938899590140112E-4 28-29 0.0 2.5271913149532394E-5 0.0 0.0019459373125139943 6.317978287383099E-4 30-31 0.0 2.5271913149532394E-5 0.0 0.007139315464742901 6.697056984626084E-4 32-33 0.0 2.5271913149532394E-5 0.0 0.019446737168565174 7.328854813364394E-4 34-35 0.0 2.5271913149532394E-5 0.0 0.03909564964232662 8.592450470841014E-4 36-37 0.0 2.5271913149532394E-5 0.0 0.06956094094408792 8.592450470841014E-4 38-39 0.0 2.5271913149532394E-5 0.0 0.1134835259979752 8.845169602336338E-4 40-41 0.0 2.5271913149532394E-5 0.0 0.16143698119921293 9.097888733831662E-4 42-43 0.0 2.5271913149532394E-5 0.0 0.21152591306158613 9.224248299579324E-4 44-45 0.0 2.5271913149532394E-5 0.0 0.2739980823672302 9.603326996822309E-4 46-47 1.2635956574766197E-5 2.5271913149532394E-5 0.0 0.34856286211492554 0.0010108765259812958 48-49 2.5271913149532394E-5 2.5271913149532394E-5 0.0 0.42540211404607875 0.001149872048303724 50-51 2.5271913149532394E-5 2.5271913149532394E-5 0.0 0.5065502271692273 0.0012130518311775549 52-53 2.5271913149532394E-5 2.5271913149532394E-5 0.0 0.5901118079981562 0.0012383237443270873 54-55 2.5271913149532394E-5 7.581573944859718E-5 0.0 0.6844771316985101 0.0012509597009018535 56-57 2.5271913149532394E-5 7.581573944859718E-5 0.0 0.7907834343620181 0.0013267754403504506 58-59 2.5271913149532394E-5 7.581573944859718E-5 0.0 0.9137186558779185 0.0013646833100747493 60-61 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.0427949522891553 0.0013773192666495155 62-63 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.173981453448378 0.001415227136373814 64-65 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.3166414031774882 0.0014404990495233464 66-67 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.4793546159907525 0.0014657709626728788 68-69 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.663081424587853 0.0014910428758224113 70-71 2.5271913149532394E-5 7.581573944859718E-5 0.0 1.8605687898948737 0.0015668586152710086 72-73 2.5271913149532394E-5 7.581573944859718E-5 0.0 2.0706415679503616 0.001731126050742969 74-75 2.5271913149532394E-5 7.581573944859718E-5 0.0 2.295511051154901 0.0018953934862149294 76-77 2.5271913149532394E-5 7.581573944859718E-5 0.0 2.5429104449322484 0.0019459373125139943 78-79 2.5271913149532394E-5 7.581573944859718E-5 0.0 2.8208130378810816 0.0019712092256635267 80-81 2.5271913149532394E-5 7.581573944859718E-5 0.0 3.1239749080228725 0.0020217530519625916 82-83 2.5271913149532394E-5 7.581573944859718E-5 0.0 3.4483904571234194 0.0021607485742850194 84-85 2.5271913149532394E-5 1.0108765259812958E-4 0.0 3.7855430504513308 0.002173384530859786 86-87 2.5271913149532394E-5 1.0108765259812958E-4 0.0 4.153009303602107 0.002249200270308383 88-89 2.5271913149532394E-5 1.0108765259812958E-4 0.0 4.551496830143934 0.0023376519663317464 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4025 0.0 54.026257 1 GTATCAA 7660 0.0 44.13229 1 CAACGCA 9810 0.0 33.812508 5 TCAACGC 9845 0.0 33.798954 4 ATCAACG 9875 0.0 33.64905 3 TATCAAC 9960 0.0 33.315914 2 AACGCAG 10525 0.0 31.740538 6 ACGCAGA 11990 0.0 27.466543 7 CGCAGAG 12355 0.0 26.578295 8 GTACATG 11115 0.0 23.2805 1 TACATGG 11375 0.0 22.494236 2 GCAGAGT 14430 0.0 22.131582 9 ACATGGG 11155 0.0 21.830301 3 AGAGTAC 12895 0.0 19.890152 10-11 CATGGGG 8275 0.0 18.9872 4 CAGAGTA 13560 0.0 18.879719 10-11 GAGTACT 8575 0.0 18.787518 12-13 GAGTACA 8535 0.0 18.635736 1 AGTACTT 9295 0.0 17.357742 12-13 GTACTTT 9360 0.0 17.034412 14-15 >>END_MODULE