FastQCFastQC Report
Sat 18 Jun 2016
SRR1930963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339504
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61420.18391952816945273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.056.0180241
GTATCAA50050.045.4719121
ATCAACG63400.035.1719133
TCAACGC64650.034.9461674
TATCAAC64400.034.628522
CAACGCA65750.034.057345
AACGCAG72650.030.5445086
GTACATG76400.028.473681
TACATGG77350.026.8829462
ACGCAGA83000.026.4737457
CGCAGAG86200.025.5378728
ACATGGG79950.024.5618233
GCAGAGT97500.021.2917259
CATGGGG57800.021.2810884
GTATTAG9200.019.6591071
AGAGTAC87500.019.0589310-11
TAAGGTG12050.018.4229555
GAGTACT57150.018.25700412-13
CAGAGTA93600.018.1954210-11
TATACTG13550.017.8081655
Produced by FastQC (version 0.11.5)