##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930963_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3339504 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.991715835645053 30.0 16.0 31.0 2.0 33.0 2 22.780735103176998 30.0 10.0 31.0 2.0 34.0 3 23.29912346264595 30.0 16.0 31.0 2.0 34.0 4 25.315460020410217 33.0 10.0 35.0 2.0 37.0 5 25.309033616968268 33.0 10.0 35.0 2.0 37.0 6 26.12649513221125 33.0 17.0 36.0 2.0 37.0 7 26.25951249047763 33.0 17.0 36.0 2.0 37.0 8 27.14960994207523 33.0 18.0 36.0 2.0 37.0 9 28.44678940345632 35.0 23.0 38.0 2.0 39.0 10-11 28.789688828041527 35.0 26.5 38.0 2.0 39.0 12-13 28.77970141673734 35.0 26.5 38.0 2.0 39.0 14-15 29.093574375116784 36.0 25.5 39.0 2.0 40.0 16-17 28.190773989191207 35.0 19.5 38.5 2.0 40.0 18-19 27.545719514035618 34.0 17.5 38.5 2.0 40.0 20-21 27.028896057618134 34.0 16.5 39.0 2.0 40.0 22-23 27.006805950823832 34.0 17.0 38.0 2.0 40.0 24-25 26.723740262026936 34.0 13.5 38.0 2.0 40.0 26-27 26.426689712005135 33.5 10.5 38.0 2.0 40.0 28-29 26.431253413680594 33.0 13.0 38.0 2.0 40.0 30-31 26.420777157326356 33.0 13.0 38.0 2.0 40.0 32-33 26.184596275375025 33.0 9.5 38.0 2.0 40.0 34-35 23.045345955567054 30.0 5.0 37.0 2.0 40.0 36-37 21.344127451262224 22.5 5.0 36.0 2.0 39.5 38-39 20.34790645556945 21.0 2.0 35.0 2.0 39.0 40-41 21.9109089253973 27.0 2.0 36.0 2.0 39.0 42-43 21.86981719440971 27.5 2.0 36.5 2.0 39.0 44-45 19.744552634163636 20.5 2.0 35.0 2.0 38.5 46-47 20.76493649955203 23.5 2.0 35.0 2.0 38.0 48-49 20.190183931506 24.0 2.0 35.0 2.0 38.5 50-51 17.910761298683877 12.0 2.0 33.5 2.0 37.5 52-53 21.154341033878083 24.5 2.0 34.5 2.0 38.0 54-55 23.08456180917885 30.5 2.0 37.0 2.0 39.0 56-57 23.369721521519363 31.0 2.0 37.0 2.0 39.0 58-59 22.997636774802487 30.5 2.0 37.0 2.0 39.0 60-61 22.703831916356442 30.0 2.0 36.5 2.0 39.0 62-63 22.232531088449065 29.0 2.0 36.0 2.0 39.0 64-65 21.726639045798418 28.5 2.0 35.5 2.0 39.0 66-67 21.31120489749376 28.0 2.0 35.0 2.0 38.0 68-69 20.850661056252665 28.0 2.0 34.5 2.0 38.0 70-71 20.246290916255827 26.0 2.0 34.0 2.0 37.0 72-73 19.77991327454616 26.0 2.0 34.0 2.0 36.5 74-75 19.23398474743555 25.5 2.0 34.0 2.0 36.0 76-77 18.666242801326185 24.5 2.0 33.0 2.0 35.5 78-79 18.169025549902024 22.5 2.0 33.0 2.0 35.0 80-81 17.656541061187532 20.5 2.0 32.0 2.0 35.0 82-83 17.183861136264547 19.5 2.0 32.0 2.0 35.0 84-85 16.654876143283552 17.5 2.0 32.0 2.0 34.0 86-87 16.084176123160805 10.0 2.0 31.0 2.0 34.0 88-89 15.514992346168771 6.5 2.0 31.0 2.0 34.0 90-91 14.915984229993438 2.0 2.0 31.0 2.0 34.0 92-93 14.257328184065658 2.0 2.0 30.5 2.0 34.0 94-95 13.558892727782329 2.0 2.0 30.0 2.0 34.0 96-97 12.77110582888956 2.0 2.0 29.0 2.0 34.0 98-99 11.876766879153312 2.0 2.0 29.0 2.0 34.0 100-101 10.61367900742146 2.0 2.0 26.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.63295800400083 1101 2 7.921844426049493 1101 3 7.47818607791999 1101 4 9.248592382951049 1101 5 9.239300423229402 1101 6 8.545055815333573 1101 7 8.226265906027717 1101 8 7.2209976463700976 1101 9 7.459231215306936 1101 10-11 6.727665640039408 1101 12-13 6.510711582988311 1101 14-15 7.149510872652069 1101 16-17 8.007545551165602 1101 18-19 8.53031408194597 1101 20-21 8.884142318249157 1101 22-23 8.6407534300896 1101 24-25 8.792579432796696 1101 26-27 8.766134973100012 1101 28-29 8.823831657792656 1101 30-31 8.411151635888242 1101 32-33 8.390340295946633 1101 34-35 11.151784391222481 1101 36-37 4.95375535852072 1101 38-39 8.437230671822828 1101 40-41 9.97425455552288 1101 42-43 10.304015897559069 1101 44-45 4.440630766660327 1101 46-47 6.075646760771914 1101 48-49 9.597657721484637 1101 50-51 -4.525934292538661 1101 52-53 5.6995998791456906 1101 54-55 8.26343566745587 1101 56-57 8.687468659387843 1101 58-59 9.018814478562177 1101 60-61 8.824973409305109 1101 62-63 9.088135937541573 1101 64-65 8.988322638421113 1101 66-67 8.85331640420058 1101 68-69 8.852277208966065 1101 70-71 8.843946777812125 1101 72-73 8.639595348512337 1101 74-75 8.839517019557217 1101 76-77 8.738426887546755 1101 78-79 8.639449531780361 1101 80-81 8.469509385118503 1101 82-83 8.3427350518682 1101 84-85 8.338975743433256 1101 86-87 8.153908830063612 1101 88-89 7.838171746368259 1101 90-91 7.813103996469669 1101 92-93 7.994613442405571 1101 94-95 7.7143176183314335 1101 96-97 7.788155130833989 1101 98-99 7.578079551968074 1101 100-101 7.131521213774084 1102 1 6.421450651532915 1102 2 7.619290240246578 1102 3 7.323514639765538 1102 4 9.049364412344712 1102 5 9.147167513837609 1102 6 8.343175111110032 1102 7 8.097538479174556 1102 8 7.230121305044715 1102 9 7.150915295901598 1102 10-11 6.647052502905378 1102 12-13 6.281790540908343 1102 14-15 6.548438336788433 1102 16-17 7.540922842449962 1102 18-19 8.02506785305663 1102 20-21 8.65742835149224 1102 22-23 8.216608548115754 1102 24-25 8.330065624421895 1102 26-27 8.382231282968288 1102 28-29 8.067083730896346 1102 30-31 7.975425717050253 1102 32-33 7.968220602546193 1102 34-35 10.644059687187752 1102 36-37 0.492877696549737 1102 38-39 3.144114930347069 1102 40-41 8.52146656300431 1102 42-43 9.665853244558463 1102 44-45 0.6491664281440563 1102 46-47 1.054034776938142 1102 48-49 7.461062917962849 1102 50-51 -5.882396325884724 1102 52-53 4.243199301112938 1102 54-55 7.274840419586898 1102 56-57 7.953578628641118 1102 58-59 8.211132898135578 1102 60-61 8.176200894562271 1102 62-63 8.210880529926929 1102 64-65 7.957151230541392 1102 66-67 8.11732394856736 1102 68-69 8.101052989565353 1102 70-71 7.9309344138040565 1102 72-73 7.981994413285474 1102 74-75 7.718415963469827 1102 76-77 7.716078894093723 1102 78-79 7.879087350684092 1102 80-81 7.836359344998531 1102 82-83 7.561587243973026 1102 84-85 7.575733887454188 1102 86-87 7.279718798220321 1102 88-89 7.253268179719276 1102 90-91 7.25673912831253 1102 92-93 7.223497773398448 1102 94-95 6.979239483311867 1102 96-97 7.069724877846923 1102 98-99 6.633596370175248 1102 100-101 6.116049040062945 1103 1 6.561412648285675 1103 2 7.758533796016273 1103 3 7.431610547835501 1103 4 9.20636633721536 1103 5 9.113690872435171 1103 6 8.405399132602437 1103 7 8.06819548680134 1103 8 7.1261464542803985 1103 9 7.082656512699245 1103 10-11 6.572835933812694 1103 12-13 6.347343647809229 1103 14-15 7.0007402939224015 1103 16-17 7.709013799359187 1103 18-19 8.306459257518473 1103 20-21 8.716230156337634 1103 22-23 8.654761090432256 1103 24-25 8.667974029848782 1103 26-27 8.574764319612104 1103 28-29 8.537633352503182 1103 30-31 8.255708931047312 1103 32-33 8.113805397826152 1103 34-35 10.701345971189557 1103 36-37 -3.4907694557963396 1103 38-39 -2.004891606420525 1103 40-41 7.1006518737371636 1103 42-43 9.641464739044 1103 44-45 0.6748556824621872 1103 46-47 0.1948699978421118 1103 48-49 7.80954647055848 1103 50-51 -6.157422495287594 1103 52-53 4.532353503161161 1103 54-55 7.842103981467144 1103 56-57 8.292217710405119 1103 58-59 8.33307875182355 1103 60-61 8.304884983422777 1103 62-63 8.256624479841332 1103 64-65 8.145290230815554 1103 66-67 8.131801817306481 1103 68-69 8.2724910879395 1103 70-71 8.187222910623102 1103 72-73 8.24870077951837 1103 74-75 8.08722624040697 1103 76-77 8.045129497142248 1103 78-79 7.888437299390638 1103 80-81 7.788593710710646 1103 82-83 7.6923206952409195 1103 84-85 7.692409867244709 1103 86-87 7.570715549555635 1103 88-89 7.489017437163957 1103 90-91 7.19824855286126 1103 92-93 7.154883808559296 1103 94-95 7.260644429816049 1103 96-97 6.999837010709086 1103 98-99 6.576004439902036 1103 100-101 6.066416385779879 1104 1 6.268295136724127 1104 2 7.515997181729638 1104 3 7.003256720196479 1104 4 8.873677616680883 1104 5 8.87072311799023 1104 6 7.988286643707209 1104 7 7.761509877933559 1104 8 6.812516925327824 1104 9 6.911382472557065 1104 10-11 6.211965095946564 1104 12-13 5.993829692384924 1104 14-15 6.624095086383189 1104 16-17 7.323174120108813 1104 18-19 7.881930225705464 1104 20-21 8.263885592851889 1104 22-23 8.145185584040657 1104 24-25 8.165830913688911 1104 26-27 8.2089540138242 1104 28-29 7.9674852174057555 1104 30-31 7.604935255859456 1104 32-33 7.402510830751456 1104 34-35 10.125538014532896 1104 36-37 -3.837901837731522 1104 38-39 -2.53593859184193 1104 40-41 6.3910124187729025 1104 42-43 8.727276778714486 1104 44-45 0.04296175133088198 1104 46-47 -0.7793345369133782 1104 48-49 7.091826050735168 1104 50-51 -6.456976346790807 1104 52-53 3.743581185249127 1104 54-55 6.7241629111022725 1104 56-57 7.123257145047532 1104 58-59 7.442228990884942 1104 60-61 7.451604775878895 1104 62-63 7.541119188236774 1104 64-65 7.467024728646805 1104 66-67 7.19842602249377 1104 68-69 7.191615486557243 1104 70-71 7.222542378488942 1104 72-73 7.119192568279264 1104 74-75 7.237277231470017 1104 76-77 7.030020947702827 1104 78-79 6.902686365098262 1104 80-81 6.7151733383360614 1104 82-83 6.762982236699823 1104 84-85 6.624661668012276 1104 86-87 6.581904918431695 1104 88-89 6.591144053473451 1104 90-91 6.401292495598389 1104 92-93 6.26505340278416 1104 94-95 5.918293508760838 1104 96-97 5.784419578477326 1104 98-99 5.440075269937729 1104 100-101 5.043252040744706 1105 1 5.90820979788657 1105 2 7.1322585781522925 1105 3 6.749393063867 1105 4 8.6332455807385 1105 5 8.46566226706657 1105 6 7.503465027078722 1105 7 7.435364989301906 1105 8 6.239631437807329 1105 9 6.2488202027298385 1105 10-11 5.720706449635184 1105 12-13 5.456094588641495 1105 14-15 5.809664671737178 1105 16-17 6.594096466659142 1105 18-19 7.229211315694176 1105 20-21 7.637174158219995 1105 22-23 7.515775347903411 1105 24-25 7.458068738080879 1105 26-27 7.517476727897094 1105 28-29 7.235342516862648 1105 30-31 6.893349387077375 1105 32-33 6.81836411835371 1105 34-35 9.386174983382556 1105 36-37 -4.2320482085769875 1105 38-39 -2.8272729950647033 1105 40-41 5.5476940660457075 1105 42-43 8.08424179899146 1105 44-45 -3.825917712561271 1105 46-47 -4.944340597927848 1105 48-49 5.269248498751139 1105 50-51 -10.925687196168251 1105 52-53 0.1989518025525676 1105 54-55 5.369043503963187 1105 56-57 6.193261515993619 1105 58-59 6.711855515773564 1105 60-61 6.594274663906674 1105 62-63 6.55841761892712 1105 64-65 6.21742697569157 1105 66-67 6.284177376740878 1105 68-69 6.221518004151413 1105 70-71 6.284970865980053 1105 72-73 6.1558019726290105 1105 74-75 5.997182901499432 1105 76-77 5.933787092394525 1105 78-79 5.73169436957598 1105 80-81 5.804992626408794 1105 82-83 5.626314437679646 1105 84-85 5.3906473917423305 1105 86-87 5.345204879645513 1105 88-89 5.532237093084133 1105 90-91 5.222295099242201 1105 92-93 5.1699166303270605 1105 94-95 4.98881078507525 1105 96-97 4.695166774863893 1105 98-99 4.430883321094264 1105 100-101 4.090301713677624 1106 1 5.832555583831812 1106 2 7.154809148783567 1106 3 6.768819571986057 1106 4 8.28392448156831 1106 5 8.188210476438293 1106 6 7.352913363300406 1106 7 7.05585994801277 1106 8 6.241916827378379 1106 9 6.311021043725333 1106 10-11 5.70497624173208 1106 12-13 5.54844008194539 1106 14-15 5.937539147179496 1106 16-17 6.533446740932991 1106 18-19 7.0520271602876825 1106 20-21 7.520833506659454 1106 22-23 7.1726756257191475 1106 24-25 7.259791876914807 1106 26-27 7.249098754500356 1106 28-29 7.097570996207626 1106 30-31 6.7230966510162595 1106 32-33 6.7069803699354615 1106 34-35 9.500061279832114 1106 36-37 -0.4377222765665536 1106 38-39 0.6235430910740405 1106 40-41 6.965187825159298 1106 42-43 8.018283535995923 1106 44-45 -3.9480516563640666 1106 46-47 -5.091979164186533 1106 48-49 4.7394312099183935 1106 50-51 -10.814910097908498 1106 52-53 -0.026873641528741388 1106 54-55 4.936527721265644 1106 56-57 6.0581955729820365 1106 58-59 6.4348428032940745 1106 60-61 6.382721291892551 1106 62-63 6.395127811478158 1106 64-65 6.2466685817041885 1106 66-67 6.318608566734397 1106 68-69 6.054164739981054 1106 70-71 5.868678987977429 1106 72-73 5.824531812407873 1106 74-75 5.80204879937612 1106 76-77 5.669039923293116 1106 78-79 5.5784626374992 1106 80-81 5.2446206389985655 1106 82-83 5.172833805620787 1106 84-85 5.010879128077118 1106 86-87 4.9193218363148485 1106 88-89 4.59280177475731 1106 90-91 4.585713459854533 1106 92-93 4.508819658816625 1106 94-95 4.092729972709064 1106 96-97 3.9656016375837453 1106 98-99 3.777331309320479 1106 100-101 3.6144023931397378 1107 1 5.7098868960131135 1107 2 7.084173490844115 1107 3 6.591597116639221 1107 4 7.666517974553219 1107 5 8.278887145557242 1107 6 6.736546020964457 1107 7 6.940524866116984 1107 8 6.088656147096042 1107 9 6.125157319594173 1107 10-11 5.659943458104028 1107 12-13 5.279716706416931 1107 14-15 5.590675205948383 1107 16-17 6.752855400536365 1107 18-19 7.096731685709475 1107 20-21 7.586419442571245 1107 22-23 7.378691020637785 1107 24-25 7.445372182036149 1107 26-27 7.393508046992306 1107 28-29 7.33882785154157 1107 30-31 7.160664149541436 1107 32-33 7.032303584211586 1107 34-35 9.636506034029697 1107 36-37 -0.48606683184534205 1107 38-39 0.5896556286350219 1107 40-41 7.102351003778864 1107 42-43 8.537501792031183 1107 44-45 -3.6968995690208413 1107 46-47 -4.769506249899589 1107 48-49 5.2234164440526385 1107 50-51 -10.728425605979936 1107 52-53 0.36969069408784705 1107 54-55 5.4933526765606935 1107 56-57 6.6706404253732465 1107 58-59 7.080732633119929 1107 60-61 7.067435958874256 1107 62-63 6.936880621311637 1107 64-65 6.929930826947359 1107 66-67 6.700303166161962 1107 68-69 6.647131008170845 1107 70-71 6.675168473972182 1107 72-73 6.633155053457106 1107 74-75 6.611532845597697 1107 76-77 6.657204024779293 1107 78-79 6.510700638890789 1107 80-81 6.254905653654728 1107 82-83 6.012390722249034 1107 84-85 6.1570831391331104 1107 86-87 5.964532672594634 1107 88-89 5.975713514801976 1107 90-91 5.870962668360248 1107 92-93 5.6459592095961515 1107 94-95 5.480599646759943 1107 96-97 5.279355955609866 1107 98-99 5.041522742387336 1107 100-101 4.608322136731646 1108 1 6.003935118632764 1108 2 6.478637682927353 1108 3 6.632065538060026 1108 4 8.209490136572999 1108 5 8.189110139113573 1108 6 7.526018858056343 1108 7 7.6151206121413075 1108 8 6.504184848339026 1108 9 5.006571510532176 1108 10-11 5.803822726983295 1108 12-13 5.178740575678891 1108 14-15 5.886537085143598 1108 16-17 6.182415371763003 1108 18-19 7.035865934367532 1108 20-21 7.72809652472451 1108 22-23 7.7945858889136055 1108 24-25 7.391409669740302 1108 26-27 7.671060568592441 1108 28-29 7.605203611964942 1108 30-31 7.260240380070048 1108 32-33 7.2634837439631745 1108 34-35 9.95565929365915 1108 36-37 -3.9934749821106372 1108 38-39 -2.579774546747533 1108 40-41 5.864779944779233 1108 42-43 8.31621131478833 1108 44-45 -3.57899370706736 1108 46-47 -4.813299811550294 1108 48-49 5.500393482934442 1108 50-51 -10.84509886551034 1108 52-53 0.5019023739185755 1108 54-55 5.669258560799914 1108 56-57 6.8099562254033295 1108 58-59 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-14.995305851995184 2313 82-83 -14.500454243546425 2313 84-85 -13.982013080893267 2313 86-87 -13.427523500216665 2313 88-89 -12.850122161800495 2313 90-91 -12.275033672085168 2313 92-93 -11.657432250113029 2313 94-95 -11.21644944314629 2313 96-97 -10.385448699734878 2313 98-99 -9.431249907623188 2313 100-101 -8.148528586780188 2314 1 -21.58023996554232 2314 2 -20.333090712134375 2314 3 -20.831458592130772 2314 4 -22.771430177680646 2314 5 -22.75393138726129 2314 6 -23.613150614446464 2314 7 -23.724946727926035 2314 8 -24.652380808226628 2314 9 -25.94516810787411 2314 10-11 -26.304342256181688 2314 12-13 -26.302881197609548 2314 14-15 -26.585965720692545 2314 16-17 -25.673785526104563 2314 18-19 -25.009308027473093 2314 20-21 -24.485147021852367 2314 22-23 -24.45597527831423 2314 24-25 -24.194589207925244 2314 26-27 -23.89193045273191 2314 28-29 -23.908965860537858 2314 30-31 -23.88594368278068 2314 32-33 -23.64392366344423 2314 34-35 -20.653207148540627 2314 36-37 -18.931185419821073 2314 38-39 -17.985358160664482 2314 40-41 -19.50727392727464 2314 42-43 -19.475735935492256 2314 44-45 -17.361681698088685 2314 46-47 -18.32466344791393 2314 48-49 -17.827328178120553 2314 50-51 -15.553853074264548 2314 52-53 -18.722283933601066 2314 54-55 -20.578075894867876 2314 56-57 -20.837771047323944 2314 58-59 -20.46849516610787 2314 60-61 -20.172911531473233 2314 62-63 -19.705011270580254 2314 64-65 -19.220786216626827 2314 66-67 -18.825758609899815 2314 68-69 -18.345974342077362 2314 70-71 -17.74967984373328 2314 72-73 -17.302311024866118 2314 74-75 -16.756365665157954 2314 76-77 -16.199249367388386 2314 78-79 -15.702344038439605 2314 80-81 -15.213654743477097 2314 82-83 -14.755121688120521 2314 84-85 -14.242806546470863 2314 86-87 -13.679565507217832 2314 88-89 -13.102747599373425 2314 90-91 -12.533201700089835 2314 92-93 -11.90188965968129 2314 94-95 -11.21644944314629 2314 96-97 -10.448167486199289 2314 98-99 -9.578857842278964 2314 100-101 -8.338726953174588 2315 1 -21.58023996554232 2315 2 -20.333090712134375 2315 3 -20.831458592130772 2315 4 -16.427744877473607 2315 5 -22.75393138726129 2315 6 -23.613150614446464 2315 7 -23.724946727926035 2315 8 -24.652380808226628 2315 9 -20.040406203112205 2315 10-11 -18.19549871876672 2315 12-13 -13.804064279537972 2315 14-15 -13.629443981562112 2315 16-17 -20.43554240276827 2315 18-19 -25.009308027473093 2315 20-21 -24.485147021852367 2315 22-23 -24.45597527831423 2315 24-25 -24.194589207925244 2315 26-27 -23.89193045273191 2315 28-29 -23.908965860537858 2315 30-31 -23.88594368278068 2315 32-33 -23.64392366344423 2315 34-35 -20.653207148540627 2315 36-37 -18.931185419821073 2315 38-39 -17.985358160664482 2315 40-41 -19.50727392727464 2315 42-43 -19.475735935492256 2315 44-45 -17.361681698088685 2315 46-47 -18.32466344791393 2315 48-49 -17.827328178120553 2315 50-51 -15.553853074264548 2315 52-53 -18.722283933601066 2315 54-55 -20.578075894867876 2315 56-57 -20.837771047323944 2315 58-59 -20.46849516610787 2315 60-61 -20.172911531473233 2315 62-63 -19.705011270580254 2315 64-65 -19.220786216626827 2315 66-67 -18.825758609899815 2315 68-69 -18.345974342077362 2315 70-71 -17.74967984373328 2315 72-73 -17.302311024866118 2315 74-75 -16.756365665157954 2315 76-77 -16.199249367388386 2315 78-79 -15.702344038439605 2315 80-81 -15.213654743477097 2315 82-83 -14.755121688120521 2315 84-85 -14.242806546470863 2315 86-87 -13.679565507217832 2315 88-89 -13.102747599373425 2315 90-91 -12.533201700089835 2315 92-93 -11.90188965968129 2315 94-95 -11.21644944314629 2315 96-97 -10.448167486199289 2315 98-99 -9.578857842278964 2315 100-101 -8.338726953174588 2316 1 -21.58023996554232 2316 2 -20.333090712134375 2316 3 -20.831458592130772 2316 4 -22.771430177680646 2316 5 -22.75393138726129 2316 6 -23.613150614446464 2316 7 -23.724946727926035 2316 8 -24.652380808226628 2316 9 -25.94516810787411 2316 10-11 -26.304342256181688 2316 12-13 -26.302881197609548 2316 14-15 -26.585965720692545 2316 16-17 -25.673785526104563 2316 18-19 -25.009308027473093 2316 20-21 -24.485147021852367 2316 22-23 -24.45597527831423 2316 24-25 -24.194589207925244 2316 26-27 -23.89193045273191 2316 28-29 -23.908965860537858 2316 30-31 -23.88594368278068 2316 32-33 -23.64392366344423 2316 34-35 -20.653207148540627 2316 36-37 -18.931185419821073 2316 38-39 -17.985358160664482 2316 40-41 -19.50727392727464 2316 42-43 -19.475735935492256 2316 44-45 -17.361681698088685 2316 46-47 -18.32466344791393 2316 48-49 -17.827328178120553 2316 50-51 -15.553853074264548 2316 52-53 -18.722283933601066 2316 54-55 -20.578075894867876 2316 56-57 -20.837771047323944 2316 58-59 -20.46849516610787 2316 60-61 -20.172911531473233 2316 62-63 -19.705011270580254 2316 64-65 -19.220786216626827 2316 66-67 -18.825758609899815 2316 68-69 -18.345974342077362 2316 70-71 -17.74967984373328 2316 72-73 -17.302311024866118 2316 74-75 -16.756365665157954 2316 76-77 -16.199249367388386 2316 78-79 -15.702344038439605 2316 80-81 -15.213654743477097 2316 82-83 -14.755121688120521 2316 84-85 -14.242806546470863 2316 86-87 -13.679565507217832 2316 88-89 -13.102747599373425 2316 90-91 -12.533201700089835 2316 92-93 -11.90188965968129 2316 94-95 -11.21644944314629 2316 96-97 -10.448167486199289 2316 98-99 -9.578857842278964 2316 100-101 -8.338726953174588 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 477891.0 3 27323.0 4 64694.0 5 31116.0 6 25212.0 7 30656.0 8 41245.0 9 51474.0 10 56373.0 11 54976.0 12 50498.0 13 47118.0 14 45266.0 15 48846.0 16 54648.0 17 58390.0 18 60570.0 19 64492.0 20 68399.0 21 73808.0 22 79248.0 23 85896.0 24 92569.0 25 100067.0 26 108936.0 27 118309.0 28 127962.0 29 139157.0 30 149091.0 31 152954.0 32 151590.0 33 151778.0 34 148166.0 35 133053.0 36 104489.0 37 53816.0 38 9336.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.864769835891096 19.398506505220826 13.559270679734455 24.17745297915362 2 15.46487942305744 20.512824390310293 40.390515954687814 23.63178023194446 3 18.59355037421729 25.62256769529605 30.056097593371245 25.727784337115413 4 12.735260527975603 16.905958555568485 38.29170315303455 32.067077763421366 5 13.410295037256653 37.64628361173064 35.14265491536605 13.800766435646663 6 30.93690282492091 36.985792888814764 18.16877842265047 13.908525863613864 7 26.887755316333944 32.459283091194955 22.867061200317533 17.785900392153568 8 25.427796479232352 35.437944394843704 20.96359456451211 18.170664561411833 9 26.261128832107673 16.094335542828517 20.875056332410733 36.76947929265308 10-11 24.80309444804861 26.208810703725575 28.819004407061204 20.169090441164606 12-13 25.13659541490925 24.140865310517142 28.65498753515477 22.067551739418835 14-15 22.422940339477684 25.526158162257595 26.277684844429576 25.773216653835146 16-17 22.25078136223898 28.527161864301586 26.93241993489729 22.28963683856215 18-19 22.21295717351769 27.661729412248548 28.590644938007237 21.534668476226525 20-21 23.015605711240774 26.867318194199267 28.399127951147342 21.717948143412613 22-23 22.743359442065223 26.75832083301698 28.200987302688237 22.297332422229562 24-25 22.539396180104564 26.894633750275617 28.215550261925582 22.350419807694237 26-27 22.398620370925087 27.147556978167287 28.064120229709953 22.389702421197676 28-29 22.460265593274823 27.31486917694567 28.050144415615712 22.174720814163788 30-31 22.700304041906882 26.9927426992017 28.050816137494607 22.25613712139681 32-33 22.504346106545995 27.131987599007388 27.86552102397719 22.49814527046943 34-35 22.887357329133255 27.104330271952605 27.851617695928216 22.156694702985927 36-37 23.352397139588614 27.10282200492658 27.428270319703955 22.116510535780844 38-39 23.072028665712352 27.23090122390434 27.47567822985055 22.221391880532753 40-41 23.080647470355643 27.00450488095385 27.69876815233023 22.21607949636027 42-43 23.09239870339768 27.088350359919293 27.28782763943538 22.531423297247642 44-45 23.335233575593207 27.231279785036627 27.199856929021003 22.233629710349156 46-47 22.774486362132073 27.21098146952155 27.394042391581657 22.620489776764718 48-49 23.187725208963215 27.378435958691366 26.927705340733322 22.506133491612097 50-51 23.482121142769167 27.356601510563017 26.84806806155985 22.31320928510797 52-53 22.626380914858142 27.373963217459224 27.313009829929808 22.686646037752826 54-55 22.35895552033899 27.33285207191583 27.480323100120884 22.82786930762429 56-57 22.396521535809637 27.479652996416586 27.321621316672378 22.802204151101396 58-59 22.477220804025006 27.402652582627653 27.4088230582596 22.71130355508774 60-61 22.466521828512263 27.446022061751325 27.477917417664564 22.609538692071855 62-63 22.476867814841494 27.337540565902984 27.38406005963574 22.801531559619782 64-65 22.281290261068335 27.238560739621352 27.395688478410353 23.084460520899956 66-67 22.34139885822297 27.390936767681495 27.30649503061564 22.961169343479902 68-69 22.378953688589164 27.34767873733653 27.352119278067516 22.921248296006787 70-71 22.28100475571038 27.177048959935362 27.263860350535612 23.278085933818645 72-73 22.32923304371041 27.311443805930953 27.18783476400752 23.17148838635112 74-75 22.30953104358204 27.452574753200707 27.11325825420033 23.124635949016916 76-77 22.293238929539445 27.240687685820518 27.191090759519902 23.27498262512013 78-79 22.219438911560218 27.280273648356868 27.160625026989678 23.339662413093233 80-81 22.328093540586753 27.37121138027675 27.056975579485552 23.243719499650943 82-83 22.416520172984782 27.25457841845233 27.153175032764032 23.175726375798856 84-85 22.31473102428048 27.305697394710847 27.00576995218699 23.37380162882168 86-87 22.16756824152145 27.394693550049983 27.006437780709703 23.431300427718867 88-89 22.204652133307874 27.21799852791046 26.9514751486711 23.625874190110572 90-91 22.17936601651258 27.20343408132468 26.99427494534385 23.622924956818892 92-93 22.276415893501387 27.05774979559998 27.02694726647256 23.638887044426067 94-95 22.078570197188423 26.99868749199511 27.117499765413744 23.80524254540272 96-97 22.074248933992653 26.78786641679714 27.049601350752038 24.088283298458173 98-99 21.88820934660225 26.89710186831331 26.984342958866957 24.23034582621748 100-101 22.116860472204042 26.943659593854225 26.811763501522286 24.12771643241945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38669.0 1 41293.0 2 30253.0 3 18044.0 4 23656.5 5 29831.0 6 31766.5 7 30510.0 8 27963.0 9 25044.0 10 22157.0 11 19413.5 12 16591.0 13 14041.0 14 11940.5 15 10157.0 16 8875.0 17 8195.0 18 7918.5 19 7997.5 20 8594.0 21 9461.0 22 10634.5 23 12433.5 24 14648.0 25 17323.5 26 20881.0 27 25353.5 28 30534.5 29 37012.5 30 44370.0 31 52197.5 32 60355.5 33 66745.5 34 71627.0 35 76678.5 36 82120.0 37 87329.5 38 91735.5 39 94977.0 40 96836.0 41 99010.0 42 101585.5 43 103735.0 44 105723.0 45 107886.5 46 109179.0 47 109388.0 48 108679.0 49 107093.5 50 103968.5 51 99836.5 52 96174.5 53 91879.0 54 86289.5 55 79227.5 56 71151.0 57 62737.0 58 54642.0 59 46774.0 60 38931.0 61 31778.5 62 25739.5 63 20336.5 64 15590.0 65 11711.5 66 8668.0 67 6495.5 68 4880.5 69 3646.0 70 2742.5 71 2050.5 72 1548.0 73 1122.5 74 794.0 75 578.5 76 409.5 77 260.0 78 160.5 79 102.0 80 55.0 81 32.0 82 27.0 83 18.0 84 12.5 85 7.0 86 4.0 87 3.0 88 2.5 89 2.0 90 2.0 91 2.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.017590636214239 2 20.793147724931607 3 16.583989718233603 4 21.759399000570145 5 21.563262089220437 6 15.61755877519536 7 14.447055610653559 8 11.62325303398349 9 2.922200422577724 10-11 6.277593918138742 12-13 3.438684307609753 14-15 7.139278767146259 16-17 11.103759720006325 18-19 14.082869791442082 20-21 14.470472261749052 22-23 12.444303106090006 24-25 14.916691221211293 26-27 12.529675065518711 28-29 12.529435508985765 30-31 13.345080586817684 32-33 11.868603840570337 34-35 18.675872225336455 36-37 23.396902653807274 38-39 22.464668405847096 40-41 17.588779651109864 42-43 17.496700108758667 44-45 22.81064792855466 46-47 12.322698221053187 48-49 20.99476449197246 50-51 20.64475742505474 52-53 10.809943632347798 54-55 12.372091783690033 56-57 11.889669843186294 58-59 12.890836483501742 60-61 12.31254701296959 62-63 12.263782286231727 64-65 13.317112361596214 66-67 11.664636425049947 68-69 12.67231600860487 70-71 11.350877256023649 72-73 10.717938951413144 74-75 10.80606581097073 76-77 10.85505512195823 78-79 11.241429865033849 80-81 11.210467183150552 82-83 9.770642586443975 84-85 9.680314801239945 86-87 9.816712302186193 88-89 11.168499873035037 90-91 8.731401429673388 92-93 10.708356690095295 94-95 10.805661559321385 96-97 10.367063492063492 98-99 10.208611817802883 100-101 10.345263847565386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3339504.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.65051233184666 #Duplication Level Percentage of deduplicated Percentage of total 1 98.734251381488 95.42715980722153 2 0.8535205679558828 1.6498640035740963 3 0.18422761833755372 0.5341708109400142 4 0.07616804285095206 0.29446721459434266 5 0.03898554495042742 0.1883986446497524 6 0.025422634784022024 0.14742664060206925 7 0.01826996419429318 0.1236060979764051 8 0.0125235592751585 0.09683267361298553 9 0.00898588577695295 0.07816414176881609 >10 0.04375722185260078 0.6918415049266737 >50 0.002132601518024913 0.14425297026822934 >100 0.0016001685693775782 0.3041306603740698 >500 6.19233816068839E-5 0.03842322788786648 >1k 6.192337676937782E-5 0.09746452137692571 >5k 3.096168838468891E-5 0.18379708022630573 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6142 0.18391952816945273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.9944566618276248E-5 7 0.0 0.0 0.0 0.0 2.9944566618276248E-5 8 0.0 0.0 0.0 0.0 2.9944566618276248E-5 9 0.0 0.0 0.0 0.0 2.9944566618276248E-5 10-11 0.0 0.0 0.0 0.0 2.9944566618276248E-5 12-13 0.0 0.0 0.0 0.0 8.983369985482874E-5 14-15 0.0 0.0 0.0 0.0 1.1977826647310499E-4 16-17 0.0 0.0 0.0 2.9944566618276248E-5 2.0961196632793372E-4 18-19 0.0 0.0 0.0 8.983369985482874E-5 2.0961196632793372E-4 20-21 0.0 0.0 0.0 1.4972283309138123E-4 2.0961196632793372E-4 22-23 0.0 0.0 0.0 2.0961196632793372E-4 2.0961196632793372E-4 24-25 0.0 0.0 0.0 2.695010995644862E-4 2.0961196632793372E-4 26-27 0.0 0.0 0.0 3.892793660375912E-4 2.0961196632793372E-4 28-29 0.0 0.0 0.0 0.001092976681567083 2.3955653294620999E-4 30-31 0.0 0.0 0.0 0.0038179322438302216 2.3955653294620999E-4 32-33 0.0 0.0 0.0 0.010585404299560654 2.695010995644862E-4 34-35 0.0 1.4972283309138124E-5 0.0 0.02082644608301113 2.695010995644862E-4 36-37 0.0 2.9944566618276248E-5 0.0 0.03671203867400668 2.695010995644862E-4 38-39 0.0 2.9944566618276248E-5 0.0 0.059874160953243355 2.8447338287362434E-4 40-41 0.0 2.9944566618276248E-5 0.0 0.08621040729401733 2.9944566618276245E-4 42-43 0.0 5.9889133236552496E-5 0.0 0.11340007378341216 2.9944566618276245E-4 44-45 0.0 5.9889133236552496E-5 0.0 0.14910896947570657 3.1441794949190057E-4 46-47 0.0 5.9889133236552496E-5 0.0 0.18868071426175864 3.4436251611017686E-4 48-49 0.0 5.9889133236552496E-5 0.0 0.2317859179087673 3.59334799419315E-4 50-51 0.0 5.9889133236552496E-5 0.0 0.2840242143743502 3.892793660375912E-4 52-53 0.0 5.9889133236552496E-5 0.0 0.34990226093455795 4.7911306589241997E-4 54-55 0.0 5.9889133236552496E-5 0.0 0.4244941763806841 5.090576325106962E-4 56-57 0.0 5.9889133236552496E-5 0.0 0.5108393342244836 5.988913323655249E-4 58-59 0.0 5.9889133236552496E-5 0.0 0.6067667533861316 6.288358989838011E-4 60-61 0.0 5.9889133236552496E-5 0.0 0.7114529582836253 6.587804656020774E-4 62-63 0.0 5.9889133236552496E-5 0.0 0.8168278882133395 7.785587320751824E-4 64-65 0.0 5.9889133236552496E-5 0.0 0.930916687028972 7.785587320751824E-4 66-67 0.0 5.9889133236552496E-5 0.0 1.0614450529180381 7.785587320751824E-4 68-69 0.0 5.9889133236552496E-5 0.0 1.2050142775693637 7.785587320751824E-4 70-71 0.0 5.9889133236552496E-5 0.0 1.358659848887739 7.935310153843205E-4 72-73 0.0 7.486141654569061E-5 0.0 1.5199113401271567 8.683924319300111E-4 74-75 0.0 8.983369985482874E-5 0.0 1.6894724486031458 9.133092818574256E-4 76-77 0.0 8.983369985482874E-5 0.0 1.879395862379563 9.282815651665637E-4 78-79 0.0 8.983369985482874E-5 0.0 2.0906098630215744 9.582261317848399E-4 80-81 0.0 8.983369985482874E-5 0.0 2.3210033585825918 0.0010031429817122543 82-83 0.0 8.983369985482874E-5 0.0 2.5565772641685713 0.0010630321149488068 84-85 0.0 8.983369985482874E-5 0.0 2.8042038578184068 0.0010780043982579449 86-87 0.0 8.983369985482874E-5 0.0 3.0710698355204844 0.001092976681567083 88-89 0.0 8.983369985482874E-5 0.0 3.3512910899343136 0.0011229212481853592 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2565 0.0 56.018024 1 GTATCAA 5005 0.0 45.471912 1 ATCAACG 6340 0.0 35.171913 3 TCAACGC 6465 0.0 34.946167 4 TATCAAC 6440 0.0 34.62852 2 CAACGCA 6575 0.0 34.05734 5 AACGCAG 7265 0.0 30.544508 6 GTACATG 7640 0.0 28.47368 1 TACATGG 7735 0.0 26.882946 2 ACGCAGA 8300 0.0 26.473745 7 CGCAGAG 8620 0.0 25.537872 8 ACATGGG 7995 0.0 24.561823 3 GCAGAGT 9750 0.0 21.291725 9 CATGGGG 5780 0.0 21.281088 4 GTATTAG 920 0.0 19.659107 1 AGAGTAC 8750 0.0 19.05893 10-11 TAAGGTG 1205 0.0 18.422955 5 GAGTACT 5715 0.0 18.257004 12-13 CAGAGTA 9360 0.0 18.19542 10-11 TATACTG 1355 0.0 17.808165 5 >>END_MODULE