FastQCFastQC Report
Sat 18 Jun 2016
SRR1930963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339504
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.1444525893665646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.12334166990067987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.055.030181
GTATCAA65900.043.445161
TCAACGC83550.033.5185284
ATCAACG84200.033.260773
CAACGCA84100.033.119095
TATCAAC86250.032.6358152
AACGCAG88550.031.454726
ACGCAGA105500.026.2661657
CGCAGAG108250.025.5988968
GTACATG100300.024.2843171
ACATGGG100150.022.8922543
TACATGG104000.022.8210452
GCAGAGT125100.021.4302589
CATGGGG70500.020.1308174
GTGGTAT19800.019.9031751
AGAGTAC114450.019.7345610-11
TATACCG2905.2850737E-817.998285
CAGAGTA121850.017.71830610-11
GAGTACA78400.017.4415841
GAGTACT73750.017.2428512-13
Produced by FastQC (version 0.11.5)