##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930963_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3339504 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.262492873193146 31.0 31.0 33.0 30.0 34.0 2 31.384694852888334 31.0 31.0 34.0 30.0 34.0 3 31.46760506949535 31.0 31.0 34.0 30.0 34.0 4 35.33743753563403 37.0 35.0 37.0 33.0 37.0 5 35.130569689391 37.0 35.0 37.0 32.0 37.0 6 35.165195789554375 37.0 35.0 37.0 32.0 37.0 7 35.044355688748986 37.0 35.0 37.0 32.0 37.0 8 35.020715351740854 36.0 35.0 37.0 32.0 37.0 9 36.52638685265836 38.0 35.0 39.0 32.0 39.0 10-11 36.45510965101404 38.0 35.0 39.0 32.0 39.0 12-13 36.35272408716983 38.0 35.0 39.0 32.0 39.0 14-15 37.37769905351214 39.0 36.0 41.0 32.0 41.0 16-17 37.340561352823656 39.0 36.0 41.0 32.0 41.0 18-19 37.3253301987361 39.0 36.0 40.0 32.0 41.0 20-21 37.28532261078292 39.0 36.0 40.0 31.5 41.0 22-23 37.158642121704304 39.0 36.0 40.0 31.0 41.0 24-25 37.04100414313024 39.0 36.0 40.0 31.0 41.0 26-27 36.77309130337919 39.0 35.5 40.0 30.5 41.0 28-29 36.58893805786728 38.0 35.0 40.0 30.0 41.0 30-31 36.35379370708944 38.0 35.0 40.0 30.0 41.0 32-33 36.11534227837427 38.0 35.0 40.0 30.0 41.0 34-35 36.31539608876049 38.0 35.0 40.0 30.0 41.0 36-37 36.37570968622885 38.0 35.0 40.0 30.0 41.0 38-39 36.34098252315314 38.0 35.0 40.0 30.0 41.0 40-41 36.24274772541072 38.0 35.0 40.0 30.0 41.0 42-43 36.1049278575501 38.0 35.0 40.0 29.5 41.0 44-45 35.92546408089345 38.0 35.0 40.0 29.0 41.0 46-47 35.71141358117852 38.0 34.0 40.0 28.0 41.0 48-49 35.51608487368184 38.0 34.0 40.0 27.5 41.0 50-51 35.24122953588317 38.0 34.0 40.0 27.0 41.0 52-53 35.02635106890125 38.0 33.5 40.0 26.5 41.0 54-55 34.8512403338939 38.0 33.0 40.0 26.0 41.0 56-57 34.59169355688749 37.5 33.0 40.0 26.0 41.0 58-59 34.345933857243466 37.0 33.0 40.0 26.0 41.0 60-61 34.03157145492264 37.0 32.5 40.0 25.5 41.0 62-63 33.722647285345374 36.0 32.0 39.0 24.5 41.0 64-65 33.40750213205314 36.0 32.0 39.0 23.5 40.5 66-67 33.06382340012169 35.5 31.0 39.0 23.0 40.0 68-69 32.6929085876226 35.0 31.0 38.5 22.5 40.0 70-71 32.28215058284104 35.0 31.0 38.0 22.0 40.0 72-73 31.859560880897284 35.0 30.5 37.0 21.5 39.5 74-75 31.33531985588279 34.0 30.0 36.5 20.0 39.0 76-77 29.968246931280813 33.0 28.5 35.0 18.5 37.5 78-79 30.72942164465142 34.0 30.0 36.0 20.0 38.0 80-81 30.73197262228163 34.0 30.0 35.5 20.0 37.0 82-83 30.46520800693756 34.0 30.0 35.0 19.0 37.0 84-85 30.151094144519668 34.0 30.0 35.0 18.0 36.5 86-87 29.8183366452024 34.0 29.0 35.0 17.0 36.0 88-89 29.48548392216329 34.0 29.0 35.0 12.0 36.0 90-91 29.156433260747704 34.0 29.0 35.0 7.0 35.0 92-93 28.809227058868622 33.5 29.0 35.0 3.5 35.0 94-95 28.40079559719048 33.0 29.0 35.0 2.0 35.0 96-97 27.938794503614908 33.0 27.5 35.0 2.0 35.0 98-99 27.45047213598187 33.0 27.0 35.0 2.0 35.0 100-101 26.240444089900777 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5132654242569537 1101 2 0.5849184447915903 1101 3 0.5654331148326719 1101 4 0.3544548055836927 1101 5 0.39935929395817027 1101 6 0.4328149690880281 1101 7 0.4969899446471402 1101 8 0.4451503884376393 1101 9 0.5678055140233411 1101 10-11 0.5396936192421222 1101 12-13 0.6528003181221536 1101 14-15 0.7234191100363674 1101 16-17 0.7337837157823657 1101 18-19 0.7686023978734866 1101 20-21 0.817334656155019 1101 22-23 0.815587143599636 1101 24-25 0.7226173131277136 1101 26-27 0.6678502159291995 1101 28-29 0.6550096446852152 1101 30-31 0.7637971694187939 1101 32-33 0.76681761940538 1101 34-35 0.6450646275945715 1101 36-37 0.764955038956991 1101 38-39 0.779195984653299 1101 40-41 0.9418662633937629 1101 42-43 0.8170203569485679 1101 44-45 0.9179017283397215 1101 46-47 0.7737214449005947 1101 48-49 0.766072965311551 1101 50-51 0.7332046447540961 1101 52-53 0.7064183139393165 1101 54-55 0.7057991960617613 1101 56-57 0.8393954227552598 1101 58-59 0.6623870456918013 1101 60-61 0.6512922707454933 1101 62-63 0.6808126491178896 1101 64-65 0.7578757047700506 1101 66-67 0.7684035058210839 1101 68-69 0.6783585042350992 1101 70-71 0.6862178293545753 1101 72-73 0.6271129156678938 1101 74-75 0.6959675658404727 1101 76-77 0.8085774021823973 1101 78-79 0.6977756734078895 1101 80-81 0.6326903035820415 1101 82-83 0.9124042737951363 1101 84-85 0.9164859747960676 1101 86-87 0.8998819777413409 1101 88-89 0.786507712223333 1101 90-91 0.8292725551429356 1101 92-93 1.03669294007436 1101 94-95 1.0609327295924835 1101 96-97 0.9808360893059564 1101 98-99 1.0362459789799452 1101 100-101 1.0036524569620724 1102 1 0.4407672252456756 1102 2 0.5080330324903244 1102 3 0.5394986388786727 1102 4 0.30908133225239 1102 5 0.42738582510848033 1102 6 0.3762522424745427 1102 7 0.4061071192855934 1102 8 0.4537913201554957 1102 9 0.6221293410921902 1102 10-11 0.573106668078843 1102 12-13 0.5548920872580396 1102 14-15 0.750545146826596 1102 16-17 0.7016172944125003 1102 18-19 0.6883769917988047 1102 20-21 0.6218557457210139 1102 22-23 0.6657274800734356 1102 24-25 0.618727916481582 1102 26-27 0.6811581041882775 1102 28-29 0.6403066159251765 1102 30-31 0.765263467448932 1102 32-33 0.8004689327326062 1102 34-35 0.8861732738184074 1102 36-37 0.9096942209862888 1102 38-39 0.9305609219416979 1102 40-41 0.9104471748680822 1102 42-43 0.8348612301193938 1102 44-45 0.8795931130104151 1102 46-47 0.9454847546959186 1102 48-49 0.9126568794667307 1102 50-51 0.8763840823497802 1102 52-53 0.9720876309557624 1102 54-55 0.8821513917423474 1102 56-57 0.7370351382395697 1102 58-59 0.844978099915032 1102 60-61 0.7211683684427541 1102 62-63 0.6472293943319798 1102 64-65 0.6358927482498018 1102 66-67 0.749113379053405 1102 68-69 0.7601840607775685 1102 70-71 0.7683857048648974 1102 72-73 0.6622045432038774 1102 74-75 0.7290475926729378 1102 76-77 0.9259887556343607 1102 78-79 0.9279042480308028 1102 80-81 0.8049740049559411 1102 82-83 0.8105164657378623 1102 84-85 0.9333443496200786 1102 86-87 0.849268711880125 1102 88-89 0.8440509109223306 1102 90-91 0.8562113415506794 1102 92-93 0.9114475836693003 1102 94-95 0.8741673387076112 1102 96-97 0.9490855282699648 1102 98-99 1.209993832930845 1102 100-101 1.4101954454066608 1103 1 0.4249617611978955 1103 2 0.5164908694602879 1103 3 0.43778673466319873 1103 4 0.2734762144057399 1103 5 0.3495742001441755 1103 6 0.49774907054809603 1103 7 0.4432595936543109 1103 8 0.4518020654097228 1103 9 0.570666966825307 1103 10-11 0.5954360704186143 1103 12-13 0.585092267153378 1103 14-15 0.651069817814367 1103 16-17 0.7433018389508561 1103 18-19 0.7113005809436928 1103 20-21 0.7809325728157503 1103 22-23 0.6885754000870108 1103 24-25 0.8041760891849137 1103 26-27 0.8734204187751473 1103 28-29 0.8793704664172282 1103 30-31 0.7950831634704727 1103 32-33 0.8125495036080892 1103 34-35 0.7142828237141785 1103 36-37 0.8822168231301148 1103 38-39 0.837452491046264 1103 40-41 0.8080761058022361 1103 42-43 0.8200470673288365 1103 44-45 0.8367243525064225 1103 46-47 0.8495651621165763 1103 48-49 0.768575844835695 1103 50-51 0.8346873024573824 1103 52-53 0.7728718339970655 1103 54-55 0.7677710257080719 1103 56-57 0.7529405045470625 1103 58-59 0.7997008753019657 1103 60-61 0.6191003322717208 1103 62-63 0.6567126252447082 1103 64-65 0.6986654600366506 1103 66-67 0.5558282839003681 1103 68-69 0.5379975151054879 1103 70-71 0.5794036530601687 1103 72-73 0.5081305504348563 1103 74-75 0.7288804553609047 1103 76-77 0.7968493452976482 1103 78-79 0.9488732690646842 1103 80-81 0.8807901981025275 1103 82-83 0.8278344808830909 1103 84-85 0.8984481695688835 1103 86-87 0.8360804256992331 1103 88-89 0.7660982824119138 1103 90-91 0.7453554702200407 1103 92-93 0.804825857576823 1103 94-95 0.9408311306600261 1103 96-97 0.7608442499391792 1103 98-99 0.9295918290887109 1103 100-101 1.0960595013576295 1104 1 0.4007428706403786 1104 2 0.49404175099043 1104 3 0.4233670772821263 1104 4 0.2760327590201257 1104 5 0.3861442075178374 1104 6 0.3853338187202979 1104 7 0.38946215774970483 1104 8 0.40949533039452035 1104 9 0.5213948712417675 1104 10-11 0.5235216589645475 1104 12-13 0.5162797343517767 1104 14-15 0.7072464482466145 1104 16-17 0.6259044712829223 1104 18-19 0.7225053310362171 1104 20-21 0.6357117498181211 1104 22-23 0.595038027925888 1104 24-25 0.6263256468118001 1104 26-27 0.6399377478129296 1104 28-29 0.5829131334455937 1104 30-31 0.7113907020377042 1104 32-33 0.814436266160321 1104 34-35 0.7939507665557102 1104 36-37 0.7600640960404377 1104 38-39 0.6869239831268033 1104 40-41 0.7124156596723878 1104 42-43 0.6936412008396644 1104 44-45 0.8182680579999371 1104 46-47 0.7582741467046006 1104 48-49 0.7074581151405255 1104 50-51 0.8465135935240653 1104 52-53 0.8160506373757173 1104 54-55 0.7526412688338269 1104 56-57 0.7651445676119124 1104 58-59 0.7524577019985372 1104 60-61 0.7609568637421589 1104 62-63 0.7058299764687277 1104 64-65 0.7725190700081583 1104 66-67 0.7400575031777876 1104 68-69 0.7224639779943445 1104 70-71 0.7306431926677348 1104 72-73 0.7581147031209241 1104 74-75 0.8361346099206877 1104 76-77 0.9813605535434711 1104 78-79 1.0028976944364203 1104 80-81 0.8395550211672678 1104 82-83 1.0054402220852694 1104 84-85 1.10178971553961 1104 86-87 1.0471911173226225 1104 88-89 1.060498727255336 1104 90-91 1.0415550512916347 1104 92-93 1.1814142012248965 1104 94-95 1.1556179330169876 1104 96-97 1.1674348215331456 1104 98-99 1.2793767717167732 1104 100-101 1.6508900423010289 1105 1 0.20712226722211824 1105 2 0.34392044608224737 1105 3 0.24908202544079927 1105 4 0.19781397878332285 1105 5 0.18087051721313685 1105 6 0.2557268823165586 1105 7 0.2776256326718425 1105 8 0.25341955379002457 1105 9 0.39518999556756285 1105 10-11 0.2743996944912155 1105 12-13 0.27007588794999293 1105 14-15 0.42586871711687735 1105 16-17 0.3562481759589744 1105 18-19 0.4015146894148671 1105 20-21 0.40556466848236994 1105 22-23 0.5010364937099681 1105 24-25 0.420078763284053 1105 26-27 0.364509988197419 1105 28-29 0.609874724353034 1105 30-31 0.540407514564123 1105 32-33 0.5770634693840933 1105 34-35 0.4402353777638268 1105 36-37 0.46505985202256994 1105 38-39 0.30708592198679696 1105 40-41 0.32123371646452625 1105 42-43 0.32132605947317217 1105 44-45 0.4010641772890722 1105 46-47 0.4712940735108688 1105 48-49 0.3965708438555495 1105 50-51 0.41960966648825604 1105 52-53 0.4509158634610202 1105 54-55 0.3871309797252138 1105 56-57 0.3670879231661459 1105 58-59 0.3252718789413862 1105 60-61 0.39496447820409486 1105 62-63 0.31827552618866406 1105 64-65 0.337185186498715 1105 66-67 0.28160964597987714 1105 68-69 0.48443961639324584 1105 70-71 0.3919742203693204 1105 72-73 0.3436811268085336 1105 74-75 0.40650172631324466 1105 76-77 0.38479653102994504 1105 78-79 0.5086706203255744 1105 80-81 0.34471723003818155 1105 82-83 0.41693233322761003 1105 84-85 0.515106208191547 1105 86-87 0.6193880000580307 1105 88-89 0.5983064108585339 1105 90-91 0.511336790152761 1105 92-93 0.5948336595814396 1105 94-95 0.36851900886052036 1105 96-97 0.17620775342254902 1105 98-99 0.3225121708875385 1105 100-101 0.5261582646921781 1106 1 0.018271515470623 1106 2 0.045609211170599906 1106 3 0.1115590950944707 1106 4 -0.004978888045123142 1106 5 -0.05044675207147975 1106 6 0.08304077151620248 1106 7 0.08525010104973774 1106 8 0.009050867498061166 1106 9 0.09399632080125997 1106 10-11 0.20251815385065441 1106 12-13 0.1117396546544498 1106 14-15 0.10206954340288377 1106 16-17 0.02749972480975771 1106 18-19 0.14437631147630725 1106 20-21 0.08015542542863585 1106 22-23 0.16076606368589097 1106 24-25 0.216222323219597 1106 26-27 0.18071577699939922 1106 28-29 0.1349473911479535 1106 30-31 0.1741347395383528 1106 32-33 0.09588549505833299 1106 34-35 0.027728947202476206 1106 36-37 0.1461129892655535 1106 38-39 0.10840451125420714 1106 40-41 0.12343224265147512 1106 42-43 0.23403896476305164 1106 44-45 0.10910186608822414 1106 46-47 0.0265551339796275 1106 48-49 0.14684155645658592 1106 50-51 0.10631983968470138 1106 52-53 0.1453761163015983 1106 54-55 0.2084253602758679 1106 56-57 0.15000684254538754 1106 58-59 0.2286612154158405 1106 60-61 0.21198056612346505 1106 62-63 0.04927239964875696 1106 64-65 0.0467993837846592 1106 66-67 0.050269770538250214 1106 68-69 0.08893301589862546 1106 70-71 0.03833426135459206 1106 72-73 0.07124542564799086 1106 74-75 0.3174928088810738 1106 76-77 0.2630467370595184 1106 78-79 0.32634213196135775 1106 80-81 0.29711385041771265 1106 82-83 0.4092154273447086 1106 84-85 0.3582744069027619 1106 86-87 0.36184789832258346 1106 88-89 0.44541543563124364 1106 90-91 0.414175837024537 1106 92-93 0.42883084232783375 1106 94-95 0.37449895673474387 1106 96-97 0.20884217973518204 1106 98-99 0.4077736145771098 1106 100-101 0.6091909116215533 1107 1 0.15440454327859854 1107 2 0.19731904595215966 1107 3 0.17985521330118104 1107 4 0.0342271398157834 1107 5 0.1324253653128551 1107 6 0.16821767729712178 1107 7 0.121327894945928 1107 8 0.12094863566446179 1107 9 0.24887013816265835 1107 10-11 0.22080529654669334 1107 12-13 0.16497122206244796 1107 14-15 0.2095952789536497 1107 16-17 0.16036117898056546 1107 18-19 0.09075745461882434 1107 20-21 0.23810389218893846 1107 22-23 0.21366990988399692 1107 24-25 0.2669473833823659 1107 26-27 0.24331221579294038 1107 28-29 0.331606084697178 1107 30-31 0.35042259106960216 1107 32-33 0.3616678565688929 1107 34-35 0.16842065221963054 1107 36-37 0.11063885599686074 1107 38-39 0.1808353480403042 1107 40-41 0.267568817704003 1107 42-43 0.2996348734644627 1107 44-45 0.26028249095253386 1107 46-47 0.09524552258854868 1107 48-49 0.16165164769226692 1107 50-51 0.06727395181107454 1107 52-53 -0.1827311172695758 1107 54-55 0.09952188383811489 1107 56-57 0.08745278810776114 1107 58-59 0.09148725851210315 1107 60-61 -0.009654442050383238 1107 62-63 -0.18423218438234557 1107 64-65 -0.2333360083415954 1107 66-67 -0.13289754141904808 1107 68-69 -0.028581005460026176 1107 70-71 -0.20607825311554961 1107 72-73 -0.21387670650374702 1107 74-75 0.01983971313538646 1107 76-77 0.019271598917203647 1107 78-79 0.04637880881305634 1107 80-81 0.18111726571458675 1107 82-83 0.23732737881251253 1107 84-85 0.2656245829992123 1107 86-87 0.1569955608157656 1107 88-89 0.252509272677397 1107 90-91 0.2580701500028937 1107 92-93 0.29591691594082903 1107 94-95 0.36788899176992373 1107 96-97 0.5376370371511427 1107 98-99 0.4712975228059655 1107 100-101 0.692674821292627 1108 1 0.24256490524848218 1108 2 0.3354240650095619 1108 3 0.3602567032264794 1108 4 0.15069982771704105 1108 5 0.25732701402402824 1108 6 0.2295470957693908 1108 7 0.3243583836907078 1108 8 0.3138372571245114 1108 9 0.4426011295127026 1108 10-11 0.40501277242083944 1108 12-13 0.3456011407876076 1108 14-15 0.377213113238156 1108 16-17 0.38559298159331945 1108 18-19 0.4226933639356929 1108 20-21 0.532674746283611 1108 22-23 0.4969531157862477 1108 24-25 0.48840833103378856 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2316 8 -0.4732947634705482 2316 9 -0.48876724511118397 2316 10-11 -0.4547778488808021 2316 12-13 -0.9650551410100476 2316 14-15 -1.7010413291537745 2316 16-17 -1.2156261924209275 2316 18-19 -0.7923390396505425 2316 20-21 -0.7671959887362831 2316 22-23 -0.5844778169741218 2316 24-25 -0.8648966600792463 2316 26-27 -1.2203883190582374 2316 28-29 -1.3266936590667129 2316 30-31 -1.0581104050120302 2316 32-33 -1.0445371038328446 2316 34-35 -1.3026310377036552 2316 36-37 -1.0357710191512055 2316 38-39 -0.9727321832370706 2316 40-41 -1.2523841572239576 2316 42-43 -1.0346019914839033 2316 44-45 -0.640136085258149 2316 46-47 -1.549266677649051 2316 48-49 -1.1690429232746737 2316 50-51 -0.6252919329449114 2316 52-53 -1.0007662137927227 2316 54-55 -1.4403940165558282 2316 56-57 -1.3229779804386226 2316 58-59 -1.2692546692114846 2316 60-61 -0.7320946710220184 2316 62-63 -1.0936912633800517 2316 64-65 -0.9263960055983631 2316 66-67 -0.7463689895208461 2316 68-69 -0.9539736761278519 2316 70-71 -0.7654170184020614 2316 72-73 -0.726488525674764 2316 74-75 -0.42563719176341763 2316 76-77 -0.8320534615518227 2316 78-79 -0.9254076600551251 2316 80-81 -0.9621878464621183 2316 82-83 -1.2400213432554992 2316 84-85 -1.1126423883750398 2316 86-87 -1.056380313343876 2316 88-89 -0.9735752920675367 2316 90-91 -0.6680197899836529 2316 92-93 -1.0628285143721357 2316 94-95 -0.833886554919463 2316 96-97 -0.6578396726390743 2316 98-99 -0.7859784179052163 2316 100-101 -0.7417604008024057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 25.0 4 54.0 5 102.0 6 228.0 7 538.0 8 1401.0 9 2516.0 10 3967.0 11 6208.0 12 8594.0 13 11135.0 14 13027.0 15 15946.0 16 18576.0 17 20995.0 18 19290.0 19 17642.0 20 17825.0 21 19119.0 22 21752.0 23 25427.0 24 31379.0 25 39088.0 26 50044.0 27 64286.0 28 81010.0 29 101854.0 30 124434.0 31 149918.0 32 180228.0 33 218343.0 34 268153.0 35 332846.0 36 430976.0 37 541175.0 38 434914.0 39 66465.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78060863093233 20.756977421562507 14.401919249551861 24.06049469795331 2 15.633574683424523 21.63727174479077 39.37226617045112 23.35688740133359 3 18.452165450121655 26.16378813400711 30.699097885083287 24.68494853078795 4 13.20582277768159 17.81943589894133 37.99378612950516 30.980955193871925 5 13.099781550527108 38.171432678184544 35.378333824931985 13.350451946356356 6 30.905257458969192 37.94938947502068 17.73725012374596 13.408102942264163 7 26.615299757089677 32.85904134266646 22.888578663178723 17.637080237065145 8 25.254498871688728 36.10491258582113 20.73580387985761 17.904784662632533 9 25.904116299905617 15.888167823724721 20.64734763006722 37.560368246302446 10-11 24.51024164067478 26.441741048970147 28.97602458329141 20.07199272706366 12-13 25.023042344012765 23.971838332878175 28.907706054551813 22.097413268557247 14-15 22.271520982541563 25.6029303766055 26.30433724783856 25.821211393014377 16-17 22.13089129403648 28.537112098084027 27.060560490420134 22.27143611745936 18-19 22.09437389504549 27.633444966677683 28.758986364442148 21.513194773834677 20-21 22.7745048366464 26.807199512262898 28.65985487665234 21.75844077443836 22-23 22.496996933825642 26.732132486161493 28.490567534964406 22.280303045048463 24-25 22.38204393791258 26.92412153370954 28.487476362373236 22.206358166004637 26-27 22.30469779364205 27.109096135879067 28.37478738950243 22.211418680976454 28-29 22.34242481137152 27.244145158213755 28.33135848739078 22.082071543023943 30-31 22.647171871698863 26.962452585276875 28.23306909009999 22.15730645292427 32-33 22.463118958300573 27.035679253618465 28.082215782734686 22.418986005346273 34-35 22.55420980250753 27.125670547195263 28.213670058435536 22.106449591861672 36-37 22.652540704346713 26.939558658502715 28.181624221154355 22.226276415996217 38-39 22.452128378727398 27.06280336884936 28.21434544345771 22.27072280896553 40-41 22.625510790151864 26.9219873786995 28.217985443042142 22.23451638810649 42-43 22.59088605896678 27.011582889020126 27.946811785689903 22.450719266323194 44-45 22.48413601216203 27.059361164071344 28.031661643326316 22.42484118044031 46-47 22.63006663172868 27.106431084824433 27.718319982031893 22.545182301414986 48-49 22.598352238577117 27.196224122146734 27.65468763897428 22.550736000301868 50-51 22.61410078780413 27.168096483053723 27.633599374989124 22.58420335415303 52-53 22.76362212483142 27.172826858569515 27.554672224303555 22.508878792295505 54-55 22.53309327664058 27.0991620527932 27.69080306630845 22.676941604257767 56-57 22.566050076501025 27.276770192362115 27.4933958159076 22.66378391522926 58-59 22.50624464637631 27.24768334102059 27.65788322960531 22.58818878299779 60-61 22.54562363220483 27.33623421881397 27.65699243779392 22.461149711187275 62-63 22.55084608722407 27.22984459139167 27.634802883879733 22.584506437504523 64-65 22.314138892456747 27.18560084961138 27.612849502872127 22.887410755059744 66-67 22.379665390467718 27.265338068818817 27.5461428055833 22.808853735130164 68-69 22.45459912473848 27.232881344195942 27.566646178251013 22.745873352814563 70-71 22.373730077472167 27.04449434484346 27.60068864149161 22.981086936192764 72-73 22.394608727780398 27.161549833444283 27.492518739297633 22.951322699477686 74-75 22.389956458374296 27.327675873796824 27.35763419330921 22.92473347451967 76-77 22.36224253890871 27.146423254868928 27.42800730367543 23.06332690254693 78-79 22.274665361793776 27.231739915035526 27.41114995516995 23.082444768000748 80-81 22.429621786302008 27.292916894559312 27.307005937610846 22.97045538152784 82-83 22.43750868392432 27.161069428274377 27.432427090969195 22.968994796832103 84-85 22.39844599677078 27.19269089062328 27.238730661798876 23.170132450807067 86-87 22.283623556073 27.28746544397012 27.257865239867957 23.171045760088923 88-89 22.246823365702937 27.12728232043016 27.28759067783021 23.338303636036695 90-91 22.242632333359335 27.118888714368133 27.347903537369394 23.290575414903138 92-93 22.310815499578982 27.005796640916298 27.41179661126596 23.271591248238764 94-95 22.11413254150859 26.950648750194457 27.40584217296242 23.529376535334535 96-97 22.094523617878583 26.79320042736886 27.49423267646932 23.618043278283242 98-99 21.921578773374726 26.86711859006607 27.396867918109997 23.814434718449206 100-101 21.945803927768914 26.820988985190613 27.395026327262972 23.8381807597775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 225.0 1 172.5 2 165.0 3 229.0 4 216.5 5 237.5 6 361.5 7 627.0 8 1028.5 9 1249.0 10 1120.0 11 990.0 12 1102.5 13 1256.0 14 1437.0 15 1639.5 16 1831.0 17 2088.0 18 2346.0 19 2669.0 20 3030.5 21 3642.5 22 4646.5 23 5812.5 24 7414.5 25 9761.0 26 13235.5 27 17772.5 28 23123.5 29 30825.5 30 40060.5 31 49728.5 32 60900.5 33 68806.0 34 74031.0 35 79399.5 36 86511.0 37 94555.0 38 99236.0 39 102766.5 40 105848.5 41 108849.0 42 112289.5 43 116025.5 44 119608.5 45 123792.0 46 127138.0 47 129121.0 48 130554.5 49 129574.5 50 126438.0 51 122644.5 52 119826.0 53 117535.0 54 112907.0 55 104534.0 56 94341.5 57 82911.0 58 71869.5 59 61770.5 60 51358.0 61 41953.0 62 34410.0 63 26518.0 64 19262.0 65 14175.5 66 10181.5 67 7708.5 68 5753.5 69 4108.5 70 3076.0 71 2255.0 72 1573.0 73 1124.5 74 840.5 75 583.0 76 362.0 77 227.5 78 141.0 79 76.0 80 37.0 81 22.0 82 16.5 83 9.0 84 5.5 85 5.0 86 3.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020931252066175094 2 0.0028447338287362436 3 0.003862849093757636 4 0.03491536467691011 5 0.0017667294304782987 6 2.9944566618276245E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.491684992741437E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.4972283309138122E-5 24-25 5.689467657472487E-4 26-27 0.0019164522635696797 28-29 8.983369985482874E-5 30-31 0.0065728323727116365 32-33 0.021829589064723383 34-35 0.013175609312041547 36-37 7.935310153843205E-4 38-39 7.635864487660443E-4 40-41 0.0020961196632793373 42-43 0.008025143853698034 44-45 0.00774067047082441 46-47 0.007605919921042166 48-49 0.009192981951810807 50-51 0.19290289815493558 52-53 0.04894439413757253 54-55 0.0170833752557266 56-57 0.00997154068388599 58-59 0.019284300902169902 60-61 0.05352591283016879 62-63 0.09685570072681451 64-65 0.10152705311926563 66-67 0.10648287889459032 68-69 0.10398250758196427 70-71 0.09222926518429084 72-73 0.06984570163712935 74-75 0.24581494736942971 76-77 0.297873576435303 78-79 0.0067674720557304325 80-81 8.833647152391494E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.486141654569061E-5 90-91 0.006003885606964387 92-93 0.017592432888237294 94-95 0.0013325332145132929 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3339504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.7601341496097 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58223636651014 46.96084987038985 2 12.220370779716157 14.605819943076161 3 3.7392728596927762 6.70378343151705 4 1.7015671421040688 4.067435227068286 5 0.9337386038859783 2.7900172114447668 6 0.601828102246253 2.1579196875144664 7 0.4060840762390907 1.6987347210447874 8 0.2894645321955923 1.383875142045009 9 0.22852731485216174 1.2291140693173752 >10 1.2156146613343053 12.842087188329426 >50 0.05921253891469175 2.38412603255906 >100 0.020375924866658075 2.1009849159789007 >500 0.0012050099617489103 0.4942093136378549 >1k 5.020874804412887E-4 0.5810432460771019 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4824 0.1444525893665646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4119 0.12334166990067987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9944566618276248E-5 2 0.0 0.0 0.0 0.0 2.9944566618276248E-5 3 0.0 0.0 0.0 0.0 2.9944566618276248E-5 4 0.0 0.0 0.0 0.0 5.9889133236552496E-5 5 0.0 0.0 0.0 2.9944566618276248E-5 5.9889133236552496E-5 6 0.0 0.0 0.0 2.9944566618276248E-5 8.983369985482874E-5 7 0.0 0.0 0.0 2.9944566618276248E-5 8.983369985482874E-5 8 0.0 0.0 0.0 2.9944566618276248E-5 8.983369985482874E-5 9 0.0 0.0 0.0 5.9889133236552496E-5 8.983369985482874E-5 10-11 0.0 0.0 0.0 8.983369985482874E-5 8.983369985482874E-5 12-13 0.0 0.0 0.0 1.1977826647310499E-4 3.892793660375912E-4 14-15 0.0 0.0 0.0 1.1977826647310499E-4 4.4916849927414374E-4 16-17 0.0 0.0 0.0 1.1977826647310499E-4 5.390021991289724E-4 18-19 0.0 0.0 0.0 1.1977826647310499E-4 5.839190490563868E-4 20-21 0.0 0.0 0.0 1.6469511640051934E-4 7.336418821477681E-4 22-23 0.0 0.0 0.0 2.0961196632793372E-4 7.486141654569062E-4 24-25 0.0 0.0 0.0 3.743070827284531E-4 7.486141654569062E-4 26-27 0.0 0.0 0.0 7.336418821477681E-4 8.085032986934587E-4 28-29 0.0 0.0 0.0 0.002051202813351923 8.53420148620873E-4 30-31 0.0 0.0 0.0 0.007695753620896995 8.983369985482875E-4 32-33 0.0 0.0 0.0 0.022129034730906147 9.582261317848399E-4 34-35 0.0 0.0 0.0 0.043973596078938665 9.582261317848399E-4 36-37 0.0 0.0 0.0 0.07674792424264201 9.582261317848399E-4 38-39 0.0 0.0 0.0 0.12418011776599161 0.0010330875483305305 40-41 0.0 0.0 0.0 0.17930806491023815 0.0010480598316396686 42-43 0.0 2.9944566618276248E-5 0.0 0.23684654966725596 0.0010630321149488068 44-45 0.0 2.9944566618276248E-5 0.0 0.30928245631686624 0.0011229212481853592 46-47 0.0 2.9944566618276248E-5 0.0 0.38936919973744605 0.0011828103814219117 48-49 0.0 2.9944566618276248E-5 0.0 0.4745465194831328 0.0011977826647310498 50-51 0.0 2.9944566618276248E-5 0.0 0.5649491661037088 0.0011977826647310498 52-53 0.0 2.9944566618276248E-5 0.0 0.6552020898911934 0.001227727231349326 54-55 0.0 2.9944566618276248E-5 0.0 0.7581964267747545 0.0012426995146584642 56-57 0.0 2.9944566618276248E-5 0.0 0.8768966888496016 0.0013175609312041548 58-59 0.0 2.9944566618276248E-5 0.0 1.0075897498550683 0.0013624777811315693 60-61 0.0 2.9944566618276248E-5 0.0 1.1486735754770767 0.0013774500644407074 62-63 0.0 2.9944566618276248E-5 0.0 1.2928117468941496 0.00143733919767726 64-65 0.0 2.9944566618276248E-5 0.0 1.4494068580244253 0.0014822560476046743 66-67 0.0 2.9944566618276248E-5 0.0 1.6284304495517898 0.0014972283309138124 68-69 0.0 2.9944566618276248E-5 0.0 1.8271875104805984 0.0014972283309138124 70-71 0.0 2.9944566618276248E-5 0.0 2.04361186571419 0.0015122006142229505 72-73 0.0 2.9944566618276248E-5 0.0 2.2702922350145407 0.0016319788806960554 74-75 0.0 2.9944566618276248E-5 0.0 2.5134720605215626 0.0017517571471691604 76-77 0.0 2.9944566618276248E-5 0.0 2.7860724227310403 0.0017966739970965747 78-79 0.0 2.9944566618276248E-5 0.0 3.0911326951547293 0.0017966739970965747 80-81 0.0 2.9944566618276248E-5 0.0 3.418127362626306 0.0018415908470239893 82-83 0.0 2.9944566618276248E-5 0.0 3.761831098270881 0.0020212582467336467 84-85 0.0 2.9944566618276248E-5 0.0 4.127663868646362 0.002036230530042785 86-87 0.0 2.9944566618276248E-5 0.0 4.526315884035474 0.0021260642298976135 88-89 0.0 2.9944566618276248E-5 0.0 4.955840747608028 0.0022458424963707185 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 55.03018 1 GTATCAA 6590 0.0 43.44516 1 TCAACGC 8355 0.0 33.518528 4 ATCAACG 8420 0.0 33.26077 3 CAACGCA 8410 0.0 33.11909 5 TATCAAC 8625 0.0 32.635815 2 AACGCAG 8855 0.0 31.45472 6 ACGCAGA 10550 0.0 26.266165 7 CGCAGAG 10825 0.0 25.598896 8 GTACATG 10030 0.0 24.284317 1 ACATGGG 10015 0.0 22.892254 3 TACATGG 10400 0.0 22.821045 2 GCAGAGT 12510 0.0 21.430258 9 CATGGGG 7050 0.0 20.130817 4 GTGGTAT 1980 0.0 19.903175 1 AGAGTAC 11445 0.0 19.73456 10-11 TATACCG 290 5.2850737E-8 17.99828 5 CAGAGTA 12185 0.0 17.718306 10-11 GAGTACA 7840 0.0 17.441584 1 GAGTACT 7375 0.0 17.24285 12-13 >>END_MODULE