FastQCFastQC Report
Sat 18 Jun 2016
SRR1930962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703705
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68250.18427493550377255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35050.040.3586121
GGTATCA20300.036.2055171
ATCAACG38750.033.3732833
TCAACGC40400.032.86734
CAACGCA39450.032.8242455
GTACATG75500.031.604531
TATCAAC43450.030.6958662
AACGCAG43400.030.244696
TACATGG77600.029.9642942
ACATGGG79750.028.3935263
CATGGGG63150.024.2126144
ACGCAGA53550.024.0193277
CGCAGAG54200.023.500958
GAGTACA51900.019.3020611
GTATAAG11000.019.2209361
GTACTAG6850.019.107361
GCAGAGT64800.018.4569849
ATGGGGG40700.018.2824025
GTATAGG10650.017.9618851
AGTACAT52650.017.7802722
Produced by FastQC (version 0.11.5)