##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930962_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3703705 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.861603718438698 30.0 10.0 31.0 2.0 33.0 2 21.734770452830343 28.0 10.0 31.0 2.0 33.0 3 22.203756238685315 28.0 10.0 31.0 2.0 33.0 4 24.145506999072552 32.0 2.0 35.0 2.0 37.0 5 24.12086248769813 32.0 10.0 35.0 2.0 37.0 6 24.854594790891824 32.0 11.0 35.0 2.0 37.0 7 24.919265708257 32.0 11.0 35.0 2.0 37.0 8 25.682949640967625 32.0 17.0 35.0 2.0 37.0 9 26.85116120209358 33.0 17.0 37.0 2.0 39.0 10-11 27.07053329031335 33.5 17.5 37.0 2.0 39.0 12-13 26.986225549821057 33.0 17.0 37.0 2.0 39.0 14-15 27.22687754559286 33.5 17.0 38.0 2.0 40.0 16-17 26.347750833287208 33.0 13.5 38.0 2.0 40.0 18-19 25.6941902770334 32.0 10.0 38.0 2.0 40.0 20-21 25.156855365100622 32.0 10.0 38.0 2.0 40.0 22-23 25.068274876103793 32.0 10.0 38.0 2.0 40.0 24-25 24.76639905176033 32.0 9.0 38.0 2.0 40.0 26-27 24.434407572957348 31.0 8.0 37.0 2.0 39.0 28-29 24.361227473570384 31.0 5.0 37.0 2.0 39.0 30-31 24.285263135157905 31.0 7.0 37.0 2.0 39.0 32-33 23.993856962150062 30.0 2.0 37.0 2.0 39.0 34-35 21.130985999154902 25.0 2.0 36.0 2.0 39.0 36-37 19.49240908765682 18.0 2.0 35.0 2.0 38.5 38-39 18.57058391529563 17.5 2.0 34.0 2.0 38.0 40-41 19.884065415577105 23.5 2.0 34.5 2.0 38.0 42-43 19.80804397218461 23.5 2.0 35.0 2.0 38.0 44-45 17.89044105834563 17.0 2.0 34.0 2.0 38.0 46-47 18.6964360012474 18.5 2.0 33.0 2.0 38.0 48-49 18.15474720583848 17.0 2.0 33.0 2.0 38.0 50-51 16.185085880219944 10.5 2.0 31.5 2.0 37.5 52-53 19.012597925590725 22.0 2.0 33.5 2.0 37.5 54-55 20.660882548691106 26.0 2.0 35.5 2.0 38.5 56-57 20.86798759620434 27.5 2.0 36.0 2.0 39.0 58-59 20.483022405942158 26.5 2.0 35.5 2.0 39.0 60-61 20.14996348251278 26.0 2.0 35.0 2.0 39.0 62-63 19.659895159036694 25.5 2.0 34.5 2.0 38.0 64-65 19.148503188023884 23.5 2.0 34.0 2.0 38.0 66-67 18.71505303473144 22.5 2.0 34.0 2.0 37.5 68-69 18.24031814088865 21.5 2.0 33.5 2.0 37.0 70-71 17.644205734527993 19.5 2.0 33.0 2.0 36.0 72-73 17.165164072192574 18.0 2.0 32.0 2.0 36.0 74-75 16.616659939169022 12.5 2.0 32.0 2.0 35.0 76-77 16.04539305911243 7.5 2.0 31.0 2.0 35.0 78-79 15.542095415266605 7.0 2.0 31.0 2.0 34.5 80-81 15.024974451258942 2.0 2.0 31.0 2.0 34.0 82-83 14.540707210752476 2.0 2.0 30.0 2.0 34.0 84-85 14.021111292611048 2.0 2.0 30.0 2.0 34.0 86-87 13.459279154252297 2.0 2.0 29.0 2.0 34.0 88-89 12.903382963815963 2.0 2.0 29.0 2.0 34.0 90-91 12.316979619057133 2.0 2.0 29.0 2.0 34.0 92-93 11.692589852593551 2.0 2.0 27.0 2.0 33.5 94-95 11.031281919051327 2.0 2.0 25.5 2.0 33.0 96-97 10.306125217856174 2.0 2.0 25.0 2.0 32.5 98-99 9.508452347041679 2.0 2.0 21.0 2.0 32.0 100-101 8.471591014943144 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.874301370873248 1101 2 7.958426273129152 1101 3 7.55373707773629 1101 4 9.487216965216259 1101 5 9.225908588104321 1101 6 8.666981115817848 1101 7 8.369029976722313 1101 8 7.457993692560041 1101 9 7.679572626676897 1101 10-11 7.206433513657895 1101 12-13 6.964320521566986 1101 14-15 7.3732840923994125 1101 16-17 8.218295242252413 1101 18-19 8.595360837904995 1101 20-21 9.045757909286461 1101 22-23 9.092750254866 1101 24-25 9.133851299453472 1101 26-27 8.94968528904862 1101 28-29 8.783636109300858 1101 30-31 8.833143733844235 1101 32-33 8.835342716874738 1101 34-35 11.1720692788473 1101 36-37 5.350674869348811 1101 38-39 8.572047394050212 1101 40-41 10.115449722071379 1101 42-43 10.498997322341314 1101 44-45 4.646619212987563 1101 46-47 6.276026942411498 1101 48-49 9.512155302785136 1101 50-51 -3.5556266658482336 1101 52-53 6.039353560992623 1101 54-55 8.706359281510181 1101 56-57 9.33527200374385 1101 58-59 9.394315311449283 1101 60-61 9.228701705969758 1101 62-63 9.28148843240923 1101 64-65 9.235352288139847 1101 66-67 9.089658275462714 1101 68-69 9.16001295281696 1101 70-71 9.200546712381083 1101 72-73 9.077483771678725 1101 74-75 8.92349217600405 1101 76-77 8.867032281841011 1101 78-79 8.829500822553419 1101 80-81 8.662563118856655 1101 82-83 8.287983921607784 1101 84-85 8.230783993138386 1101 86-87 8.02471891564734 1101 88-89 7.842908377082944 1101 90-91 7.722179482282522 1101 92-93 7.736869892758049 1101 94-95 7.407089622660521 1101 96-97 7.235005456275868 1101 98-99 6.859180318598876 1101 100-101 6.131584240732861 1102 1 6.5135758021621974 1102 2 7.559813361539835 1102 3 7.341698360252927 1102 4 9.178438793733914 1102 5 9.146529585041105 1102 6 8.442339531012927 1102 7 8.19447097385115 1102 8 7.253084868776444 1102 9 7.218826892609929 1102 10-11 6.865440705968172 1102 12-13 6.550042729385385 1102 14-15 6.984543082787265 1102 16-17 7.667534480194011 1102 18-19 8.071318932374883 1102 20-21 8.364833615794108 1102 22-23 8.15772471208184 1102 24-25 8.281798274169955 1102 26-27 8.404513691472307 1102 28-29 8.077266977659491 1102 30-31 8.083920580174624 1102 32-33 8.084650853115722 1102 34-35 10.358550328214012 1102 36-37 1.1264990082401027 1102 38-39 3.230839969358211 1102 40-41 8.259668746644305 1102 42-43 9.277174806281046 1102 44-45 1.1686563460501027 1102 46-47 1.5997454820556243 1102 48-49 7.443648547390904 1102 50-51 -4.754609995820674 1102 52-53 4.861209926043177 1102 54-55 7.769078579755792 1102 56-57 8.220583702531048 1102 58-59 8.40912268428751 1102 60-61 8.299181305351357 1102 62-63 8.338416432486046 1102 64-65 8.14855123377981 1102 66-67 8.277763356172148 1102 68-69 8.160591746544572 1102 70-71 7.994703080312405 1102 72-73 7.989132865678091 1102 74-75 7.750385798950308 1102 76-77 7.62053721275066 1102 78-79 7.494900672753632 1102 80-81 7.382257229612472 1102 82-83 7.193301573976255 1102 84-85 7.15526113426707 1102 86-87 7.152050895021061 1102 88-89 7.065859432883363 1102 90-91 7.031105292977843 1102 92-93 6.808744022526918 1102 94-95 6.353639906415889 1102 96-97 6.195255938804024 1102 98-99 5.830312171218932 1102 100-101 5.1859140307250815 1103 1 6.545717637638351 1103 2 7.650434394207043 1103 3 7.246834161531048 1103 4 8.871239359703768 1103 5 8.799545846625296 1103 6 8.177347794789178 1103 7 7.887253183317615 1103 8 7.106351019987134 1103 9 6.955676970223099 1103 10-11 6.6055967965421125 1103 12-13 6.500099406764473 1103 14-15 6.919672673671723 1103 16-17 7.584392818233706 1103 18-19 7.976171715227498 1103 20-21 8.218340042915099 1103 22-23 8.22750170066396 1103 24-25 8.248401564003732 1103 26-27 8.169855708637645 1103 28-29 8.000041301868833 1103 30-31 7.76528966715296 1103 32-33 7.734592132403222 1103 34-35 9.871066448774705 1103 36-37 -3.2540255120957546 1103 38-39 -2.0134700170485935 1103 40-41 6.183343778918921 1103 42-43 8.574532575202145 1103 44-45 0.5443213170850143 1103 46-47 0.21587867121671422 1103 48-49 6.842341718436668 1103 50-51 -5.231887893853649 1103 52-53 3.984702398201925 1103 54-55 7.0936885543045705 1103 56-57 7.760105890787038 1103 58-59 7.996047192832329 1103 60-61 8.065557249220653 1103 62-63 7.885422848552945 1103 64-65 7.796306514791748 1103 66-67 7.596102842953794 1103 68-69 7.629931126804902 1103 70-71 7.785725745234529 1103 72-73 7.454901638050078 1103 74-75 7.3127471286310985 1103 76-77 7.2263653690810745 1103 78-79 7.197524078367374 1103 80-81 6.928070070991515 1103 82-83 6.742121364698717 1103 84-85 6.631125447549609 1103 86-87 6.451250846249037 1103 88-89 6.413469469737057 1103 90-91 6.275726301527014 1103 92-93 6.251094083726427 1103 94-95 5.932375706086141 1103 96-97 5.40395156184988 1103 98-99 5.204566069798581 1103 100-101 4.957331749038508 1104 1 6.43950465722202 1104 2 7.577140887586545 1104 3 6.959704373885124 1104 4 8.783290243813187 1104 5 8.74276384237967 1104 6 7.917410375848235 1104 7 7.666918039018459 1104 8 6.8041845340206315 1104 9 6.972341225351915 1104 10-11 6.36425705842851 1104 12-13 6.161893808957455 1104 14-15 6.765700408339125 1104 16-17 7.30896113644042 1104 18-19 7.804952560613568 1104 20-21 8.098125772821543 1104 22-23 7.964138477475142 1104 24-25 7.983178120155902 1104 26-27 8.010618263334965 1104 28-29 7.895174620270456 1104 30-31 7.411714215419064 1104 32-33 7.213286624913248 1104 34-35 9.740345636806605 1104 36-37 -3.348778104829295 1104 38-39 -2.2411994649649927 1104 40-41 5.692719672922255 1104 42-43 7.9322173204743756 1104 44-45 0.24359223981867828 1104 46-47 -0.6521964987306355 1104 48-49 6.236666246683708 1104 50-51 -5.551652560793844 1104 52-53 3.170701042455164 1104 54-55 6.1388540155326545 1104 56-57 6.749414552436388 1104 58-59 6.904883827329741 1104 60-61 6.784651178289728 1104 62-63 6.761648752169393 1104 64-65 6.883842200596778 1104 66-67 6.628059089068078 1104 68-69 6.658193062100363 1104 70-71 6.704445159171371 1104 72-73 6.4678685431616785 1104 74-75 6.493571108170688 1104 76-77 6.377985988588636 1104 78-79 6.1097097773532685 1104 80-81 5.978612039348173 1104 82-83 5.734907298760005 1104 84-85 5.619968649358329 1104 86-87 5.470852024136624 1104 88-89 5.211730949152765 1104 90-91 5.217746489732681 1104 92-93 4.922384362189677 1104 94-95 4.744307400762986 1104 96-97 4.451185197583452 1104 98-99 4.17946701505535 1104 100-101 3.796870795139844 1105 1 5.9291659389453635 1105 2 6.919710603281409 1105 3 6.757398960643457 1105 4 8.576881157710634 1105 5 8.347922920496014 1105 6 7.552591928787152 1105 7 7.498993093477882 1105 8 6.404461723933732 1105 9 6.459787882363852 1105 10-11 5.9822230498247855 1105 12-13 5.657155607326597 1105 14-15 6.034583262189155 1105 16-17 6.794563761445463 1105 18-19 7.482436053282097 1105 20-21 7.75171514684747 1105 22-23 7.408664214647889 1105 24-25 7.714045509836357 1105 26-27 7.618528997986672 1105 28-29 7.399598230451652 1105 30-31 7.18284761562359 1105 32-33 6.984412136698875 1105 34-35 9.205359847043528 1105 36-37 -3.6705814273791812 1105 38-39 -2.4692870670955713 1105 40-41 5.280732457637132 1105 42-43 7.5168160170468 1105 44-45 -3.3547711935915263 1105 46-47 -4.4054362593347225 1105 48-49 4.499796650332144 1105 50-51 -9.708925989430575 1105 52-53 0.16528420862138304 1105 54-55 4.842052319561709 1105 56-57 5.989643963304488 1105 58-59 6.28329128202903 1105 60-61 6.256186535813082 1105 62-63 6.413524579068014 1105 64-65 6.251193938415458 1105 66-67 6.0745173258878005 1105 68-69 6.2171233651160485 1105 70-71 6.258800114030709 1105 72-73 6.062979307645957 1105 74-75 5.900868136870578 1105 76-77 5.733049916726573 1105 78-79 5.5984863174823065 1105 80-81 5.543776475070013 1105 82-83 5.251221755128638 1105 84-85 5.194775275525897 1105 86-87 5.183034846133081 1105 88-89 5.104425015014675 1105 90-91 4.839345098033041 1105 92-93 4.550960668169578 1105 94-95 4.330447077150055 1105 96-97 4.209728224866559 1105 98-99 3.85002480189619 1105 100-101 3.4584619270445085 1106 1 5.667993221135813 1106 2 6.754519604078553 1106 3 6.371193421976113 1106 4 7.813098854615042 1106 5 7.669796450577913 1106 6 6.869402462607155 1106 7 6.514761082403357 1106 8 6.12481531482597 1106 9 5.958581440604359 1106 10-11 5.430912194018926 1106 12-13 5.375820511633936 1106 14-15 5.564822457154303 1106 16-17 6.32674048796299 1106 18-19 6.439599999290593 1106 20-21 6.896048451035071 1106 22-23 6.565313015194725 1106 24-25 6.66549237195812 1106 26-27 6.858620896388484 1106 28-29 6.646610495272736 1106 30-31 6.260795030769625 1106 32-33 6.21049353370265 1106 34-35 8.802901138778036 1106 36-37 -0.1826263471188625 1106 38-39 0.6215265710638072 1106 40-41 6.227231810793388 1106 42-43 7.377759962149007 1106 44-45 -3.5191142435156078 1106 46-47 -4.653444255050939 1106 48-49 4.012187585201648 1106 50-51 -9.71231740360906 1106 52-53 -0.41371277192352096 1106 54-55 4.049134476694636 1106 56-57 5.12404417928073 1106 58-59 5.506068925424426 1106 60-61 5.388277995114262 1106 62-63 5.502385751147596 1106 64-65 5.58923064799443 1106 66-67 5.5188606183383975 1106 68-69 5.36169677055209 1106 70-71 5.307633944437022 1106 72-73 5.057766168325326 1106 74-75 4.881139466598778 1106 76-77 4.5565068896510414 1106 78-79 4.414589822692479 1106 80-81 4.447113416517093 1106 82-83 4.084611816264468 1106 84-85 4.176115955710648 1106 86-87 4.041638378467928 1106 88-89 4.0132959647421185 1106 90-91 3.8008712663247444 1106 92-93 3.7228669393308156 1106 94-95 3.2720021124791465 1106 96-97 2.9957177553799053 1106 98-99 2.94933666482531 1106 100-101 2.6502414908669554 1107 1 5.773897645879359 1107 2 6.986327060866007 1107 3 6.5477048585893165 1107 4 7.821955056511726 1107 5 8.230297126099416 1107 6 6.669678266875657 1107 7 6.87029634848049 1107 8 6.2257177717595305 1107 9 6.332321031884316 1107 10-11 5.855692299801927 1107 12-13 5.54193330040928 1107 14-15 5.687317478563951 1107 16-17 6.49171306608007 1107 18-19 6.876992927733806 1107 20-21 7.270921134039849 1107 22-23 7.369841029907601 1107 24-25 7.192705256668081 1107 26-27 7.274199951678039 1107 28-29 7.086650050429547 1107 30-31 7.016279935832895 1107 32-33 6.693827644212124 1107 34-35 8.965899067759057 1107 36-37 -0.034004871068631815 1107 38-39 0.6521674864653839 1107 40-41 6.61575192442276 1107 42-43 7.689139375188283 1107 44-45 -3.334770208155959 1107 46-47 -4.3794744020242735 1107 48-49 4.660055262570342 1107 50-51 -9.574990286441363 1107 52-53 0.29797253764438736 1107 54-55 4.9970040192860665 1107 56-57 6.135104639232221 1107 58-59 6.625757563793176 1107 60-61 6.522821080826841 1107 62-63 6.544301378418897 1107 64-65 6.427753466196766 1107 66-67 6.343749342258697 1107 68-69 6.067314106429588 1107 70-71 6.271739908984117 1107 72-73 6.174633843828797 1107 74-75 6.189754526282794 1107 76-77 6.100222892080803 1107 78-79 6.114494198940237 1107 80-81 5.870916953849838 1107 82-83 5.505801243933304 1107 84-85 5.429785128614929 1107 86-87 5.237309103237527 1107 88-89 5.002461756308454 1107 90-91 4.967862108702851 1107 92-93 4.773381771892881 1107 94-95 4.446957860069745 1107 96-97 4.336457328629379 1107 98-99 4.2626801884921885 1107 100-101 3.965229830116156 1108 1 5.960396471642667 1108 2 6.311142947520398 1108 3 6.642409282330632 1108 4 8.032842213624136 1108 5 8.093755398514144 1108 6 7.382833994972451 1108 7 7.381990364826201 1108 8 6.367960773977085 1108 9 4.8668557278107585 1108 10-11 5.788846103794466 1108 12-13 5.309193085849337 1108 14-15 5.879481316041112 1108 16-17 6.045733501753308 1108 18-19 6.667401565573375 1108 20-21 7.384539149259364 1108 22-23 7.4219458226752515 1108 24-25 7.166456079712351 1108 26-27 7.4716565945549895 1108 28-29 7.345614584799421 1108 30-31 7.168661802092526 1108 32-33 7.182046038971119 1108 34-35 9.48280611108401 1108 36-37 -3.479862629182197 1108 38-39 -2.329946867315151 1108 40-41 5.344383116409066 1108 42-43 7.90831425184167 1108 44-45 -3.0687932002099796 1108 46-47 -4.103277759809352 1108 48-49 5.075703716077744 1108 50-51 -9.668852759602153 1108 52-53 0.4940328721980123 1108 54-55 5.349362894235529 1108 56-57 6.613782725060911 1108 58-59 7.051069992080933 1108 60-61 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-11.693646312143674 2313 86-87 -11.13476206356706 2313 88-89 -10.57796391635112 2313 90-91 -9.998757658648117 2313 92-93 -9.417838689128686 2313 94-95 -8.861164345593444 2313 96-97 -8.125156679666503 2313 98-99 -7.339665843186167 2313 100-101 -6.324601900823892 2314 1 -20.610354713782836 2314 2 -19.46300229829942 2314 3 -19.898643874644662 2314 4 -21.810931182931892 2314 5 -21.76324485104912 2314 6 -22.52058502174829 2314 7 -22.569594361902023 2314 8 -23.35153011696377 2314 9 -24.503496156391886 2314 10-11 -24.75732310009872 2314 12-13 -24.663854081607614 2314 14-15 -24.86534024622492 2314 16-17 -24.00617565721849 2314 18-19 -23.368660326116167 2314 20-21 -22.822099233570686 2314 22-23 -22.724048686932935 2314 24-25 -22.429376213774045 2314 26-27 -22.105208890845557 2314 28-29 -22.027607276942533 2314 30-31 -21.96235891165841 2314 32-33 -21.663599082067062 2314 34-35 -18.915496858718836 2314 36-37 -17.268108199434256 2314 38-39 -16.382979060976243 2314 40-41 -17.653994722373067 2314 42-43 -17.58632013797615 2314 44-45 -15.691079199710853 2314 46-47 -16.443682052297497 2314 48-49 -15.945384379754547 2314 50-51 -13.990415025636594 2314 52-53 -16.760646439007377 2314 54-55 -18.36625976610887 2314 56-57 -18.552426408954563 2314 58-59 -18.17565823352426 2314 60-61 -17.864287712019657 2314 62-63 -17.36044278452199 2314 64-65 -16.865838188050628 2314 66-67 -16.41655865575422 2314 68-69 -15.966838899034894 2314 70-71 -15.37008820825384 2314 72-73 -14.893547974353023 2314 74-75 -14.377433749921874 2314 76-77 -13.798311633502903 2314 78-79 -13.301054733002138 2314 80-81 -12.787304606011068 2314 82-83 -12.31188380325994 2314 84-85 -11.798977911623519 2314 86-87 -11.2399636240352 2314 88-89 -10.684075749901185 2314 90-91 -10.108640623537584 2314 92-93 -9.507045451157294 2314 94-95 -8.861164345593444 2314 96-97 -8.139200892930482 2314 98-99 -7.372565713147155 2314 100-101 -6.361272902124282 2315 1 -20.610354713782836 2315 2 -19.46300229829942 2315 3 -19.898643874644662 2315 4 -16.703039850461 2315 5 -21.76324485104912 2315 6 -22.52058502174829 2315 7 -22.569594361902023 2315 8 -23.35153011696377 2315 9 -19.756536259884772 2315 10-11 -18.16327394097841 2315 12-13 -14.649753176044872 2315 14-15 -14.472196649847172 2315 16-17 -19.753523652043846 2315 18-19 -23.368660326116167 2315 20-21 -22.822099233570686 2315 22-23 -22.724048686932935 2315 24-25 -22.429376213774045 2315 26-27 -22.105208890845557 2315 28-29 -22.027607276942533 2315 30-31 -21.96235891165841 2315 32-33 -21.663599082067062 2315 34-35 -18.915496858718836 2315 36-37 -17.268108199434256 2315 38-39 -16.382979060976243 2315 40-41 -17.653994722373067 2315 42-43 -17.58632013797615 2315 44-45 -15.691079199710853 2315 46-47 -16.443682052297497 2315 48-49 -15.945384379754547 2315 50-51 -13.990415025636594 2315 52-53 -16.760646439007377 2315 54-55 -18.36625976610887 2315 56-57 -18.552426408954563 2315 58-59 -18.17565823352426 2315 60-61 -17.864287712019657 2315 62-63 -17.36044278452199 2315 64-65 -16.865838188050628 2315 66-67 -16.41655865575422 2315 68-69 -15.966838899034894 2315 70-71 -15.37008820825384 2315 72-73 -14.893547974353023 2315 74-75 -14.377433749921874 2315 76-77 -13.798311633502903 2315 78-79 -13.301054733002138 2315 80-81 -12.787304606011068 2315 82-83 -12.31188380325994 2315 84-85 -11.798977911623519 2315 86-87 -11.2399636240352 2315 88-89 -10.684075749901185 2315 90-91 -10.108640623537584 2315 92-93 -9.507045451157294 2315 94-95 -8.861164345593444 2315 96-97 -8.139200892930482 2315 98-99 -7.372565713147155 2315 100-101 -6.361272902124282 2316 1 -20.610354713782836 2316 2 -19.46300229829942 2316 3 -19.898643874644662 2316 4 -21.810931182931892 2316 5 -21.76324485104912 2316 6 -22.52058502174829 2316 7 -22.569594361902023 2316 8 -23.35153011696377 2316 9 -24.503496156391886 2316 10-11 -24.75732310009872 2316 12-13 -24.663854081607614 2316 14-15 -24.86534024622492 2316 16-17 -24.00617565721849 2316 18-19 -23.368660326116167 2316 20-21 -22.822099233570686 2316 22-23 -22.724048686932935 2316 24-25 -22.429376213774045 2316 26-27 -22.105208890845557 2316 28-29 -22.027607276942533 2316 30-31 -21.96235891165841 2316 32-33 -21.663599082067062 2316 34-35 -18.915496858718836 2316 36-37 -17.268108199434256 2316 38-39 -16.382979060976243 2316 40-41 -17.653994722373067 2316 42-43 -17.58632013797615 2316 44-45 -15.691079199710853 2316 46-47 -16.443682052297497 2316 48-49 -15.945384379754547 2316 50-51 -13.990415025636594 2316 52-53 -16.760646439007377 2316 54-55 -18.36625976610887 2316 56-57 -18.552426408954563 2316 58-59 -18.17565823352426 2316 60-61 -17.864287712019657 2316 62-63 -17.36044278452199 2316 64-65 -16.865838188050628 2316 66-67 -16.41655865575422 2316 68-69 -15.966838899034894 2316 70-71 -15.37008820825384 2316 72-73 -14.893547974353023 2316 74-75 -14.377433749921874 2316 76-77 -13.798311633502903 2316 78-79 -13.301054733002138 2316 80-81 -12.787304606011068 2316 82-83 -12.31188380325994 2316 84-85 -11.798977911623519 2316 86-87 -11.2399636240352 2316 88-89 -10.684075749901185 2316 90-91 -10.108640623537584 2316 92-93 -9.507045451157294 2316 94-95 -8.861164345593444 2316 96-97 -8.139200892930482 2316 98-99 -7.372565713147155 2316 100-101 -6.361272902124282 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 610176.0 3 38263.0 4 79465.0 5 49635.0 6 43475.0 7 53361.0 8 68723.0 9 80931.0 10 83418.0 11 76596.0 12 66037.0 13 58988.0 14 57893.0 15 61690.0 16 67130.0 17 73515.0 18 77116.0 19 81317.0 20 85568.0 21 89865.0 22 95193.0 23 101045.0 24 106619.0 25 112397.0 26 119419.0 27 126101.0 28 132612.0 29 139270.0 30 143992.0 31 142078.0 32 136630.0 33 131281.0 34 119781.0 35 98199.0 36 65844.0 37 26961.0 38 3110.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18367352082793 19.27343349022823 12.865801460480478 24.677091528463365 2 15.905941133833062 20.219515490067938 39.91107191667861 23.96347145942039 3 19.240638978883496 24.734444945268272 29.44256138301144 26.58235469283679 4 12.900157025456464 16.160736272602584 37.663290324126805 33.27581637781415 5 13.795291537631934 37.39991433110335 35.08117058645766 13.723623544807051 6 32.347374277364224 36.24156436122806 17.714319140258272 13.69674222114945 7 28.240773611794364 31.513371099356423 22.220767678099595 18.025087610749623 8 26.042407484611424 35.6507651372097 20.261268607303137 18.04555877087574 9 26.63624911700412 15.601041169735048 19.93964733603831 37.82306237722252 10-11 25.574573827564073 25.538689115749914 28.536296224472363 20.35044083221365 12-13 25.776781168106943 23.137733760539714 28.289298138758678 22.796186932594665 14-15 22.90573818967029 24.67292499503284 25.468500640632346 26.95283617466452 16-17 23.261545350560418 27.615202196895044 26.09735851700446 23.02589393554008 18-19 23.159523333234468 27.114587191915547 27.309832766549018 22.416056708300967 20-21 23.816219604749374 26.3839804446843 27.2201515638434 22.57964838672292 22-23 23.624623120011403 26.129169516629307 26.98428987598851 23.261917487370777 24-25 23.47195394824915 26.344833521390733 26.94218669318595 23.241025837174167 26-27 23.342864227581707 26.59134983451681 26.843450531685125 23.222335406216356 28-29 23.325947097655558 26.813119974766064 26.84673837248844 23.014194555089944 30-31 23.557634618239753 26.618296700726518 26.71799971933592 23.1060689616978 32-33 23.434984842367694 26.701801296800866 26.61055416451297 23.252659696318474 34-35 23.742988721929876 26.63897170144794 26.680072297031423 22.93796727959076 36-37 24.182855081729993 26.600660486980267 26.28049738662795 22.935987044661797 38-39 23.93933450889095 26.649788509848634 26.348591963881606 23.06228501737881 40-41 23.904842687058036 26.495187199570264 26.585916815112366 23.014053298259334 42-43 23.966060307085158 26.464491993998067 26.32483607736737 23.244611621549403 44-45 24.179500535943014 26.624853787744048 26.212864637841328 22.982781038471614 46-47 23.64623952073223 26.489449548800607 26.481334173828547 23.38297675663861 48-49 24.028652938035076 26.569593615315988 26.190343357696012 23.21141008895292 50-51 24.200760965038295 26.681251145825634 26.12747532541448 22.990512563721587 52-53 23.39440121317579 26.580764772213612 26.61007954175395 23.41475447285665 54-55 23.164774206180372 26.488013076559398 26.76772574239617 23.57948697486406 56-57 23.245587251055337 26.668187459977265 26.5939526469264 23.492272642040994 58-59 23.293882817510408 26.58391235053933 26.688150232029667 23.4340545999206 60-61 23.26105027756112 26.660397312586348 26.7095814464216 23.368970963430932 62-63 23.251058307326268 26.58702839662578 26.58922225998826 23.572691036059698 64-65 23.083044294867875 26.501958663420034 26.609342246619082 23.805654795093012 66-67 23.193596915382944 26.577754372267908 26.497103568153612 23.731545144195536 68-69 23.18453752432955 26.4985573059443 26.63610386900363 23.680801300722525 70-71 23.046112216338095 26.32926301399678 26.59458372206483 24.0300410476003 72-73 23.19864187082385 26.43938914537685 26.498876344475196 23.863092639324105 74-75 23.186884162187155 26.611393542577684 26.36858248163385 23.83313981360131 76-77 23.16483285340654 26.358687764093354 26.49320506037928 23.983274322120824 78-79 23.097682289575324 26.412732185557296 26.446931567342403 24.04265395752498 80-81 23.18209005976889 26.59110378993146 26.376194641358435 23.85061150894122 82-83 23.33488202776391 26.4052750683965 26.43156034091293 23.828282562926656 84-85 23.241063422334797 26.40149446750971 26.317058732927073 24.040383377228416 86-87 23.05397153815099 26.453371013322567 26.42432401907069 24.068333429455755 88-89 23.166568611056523 26.25153644189961 26.34645406908404 24.235440877959824 90-91 23.147432462691054 26.27950998938895 26.30528455821395 24.26777298970605 92-93 23.213042804669147 26.112618980889767 26.437675477323356 24.23666273711773 94-95 23.10991158062059 26.028939019095702 26.473101860474017 24.388047539809683 96-97 23.0705437213038 25.90962553961478 26.381233965721595 24.63859677335983 98-99 22.930145460433558 25.97518521151001 26.31522317660621 24.77944615145022 100-101 23.33981959637102 25.958831493594648 26.073644241843784 24.627704668190553 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41688.0 1 45924.0 2 33994.0 3 18784.5 4 24793.0 5 32474.5 6 34890.5 7 33699.0 8 31085.5 9 28232.0 10 25704.0 11 22575.5 12 19120.5 13 16073.5 14 13308.5 15 10914.5 16 9107.5 17 7827.0 18 7074.0 19 6768.5 20 6820.0 21 7265.5 22 8110.0 23 9243.5 24 10547.5 25 12262.0 26 14609.5 27 17886.5 28 22135.5 29 27594.0 30 34454.5 31 42751.0 32 52421.0 33 61162.0 34 69711.0 35 78822.5 36 87797.0 37 96327.5 38 101378.0 39 104303.0 40 106979.5 41 108938.0 42 110989.0 43 113964.5 44 117103.0 45 119968.0 46 122558.5 47 124545.0 48 125751.0 49 125007.5 50 122194.5 51 119127.5 52 116411.5 53 112437.0 54 106420.5 55 98375.0 56 89124.5 57 79300.5 58 70137.0 59 61208.0 60 51804.0 61 43148.5 62 35243.0 63 28045.5 64 21686.0 65 16380.0 66 12289.5 67 9330.0 68 7070.0 69 5296.0 70 4016.0 71 2971.5 72 2121.0 73 1520.0 74 1120.5 75 795.0 76 543.0 77 364.5 78 245.0 79 156.0 80 81.5 81 47.5 82 35.5 83 20.5 84 12.5 85 10.0 86 6.0 87 2.5 88 1.5 89 0.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.305080993221653 2 21.254149561047655 3 17.036724037146588 4 22.262950207967428 5 22.090771268230057 6 15.993498402275561 7 14.818701813454366 8 11.92171082740121 9 2.9922199527230164 10-11 6.437904746733339 12-13 3.516924269076506 14-15 7.321290437548347 16-17 11.364026022590892 18-19 14.427795950271419 20-21 14.849576302648293 22-23 12.78178202637629 24-25 15.291849647852624 26-27 12.836646007173897 28-29 12.8602439989146 30-31 13.708259702109105 32-33 12.191791732872893 34-35 19.034048338083082 36-37 23.797616170834342 38-39 22.835795507471573 40-41 17.97003271048855 42-43 17.90146623448682 44-45 23.260991358653023 46-47 12.66580356696875 48-49 21.35657402519909 50-51 21.06131562853953 52-53 11.119919108028311 54-55 12.710097051466033 56-57 12.236625217181174 58-59 13.253255861360449 60-61 12.660889568688653 62-63 12.619930583024297 64-65 13.682366711171651 66-67 12.020746792738622 68-69 13.050202432429147 70-71 11.67428291400098 72-73 11.039715636099528 74-75 11.125373106119413 76-77 11.172420589652795 78-79 11.577379947917018 80-81 11.547072458524639 82-83 10.06944397569461 84-85 9.984826005310898 86-87 10.114655459870589 88-89 11.519626968130561 90-91 9.02081834271358 92-93 11.031804638868376 94-95 11.130260104408963 96-97 10.68006226197821 98-99 10.540755810735467 100-101 10.64889077288823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3703705.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.93802877544694 #Duplication Level Percentage of deduplicated Percentage of total 1 98.78755650074534 95.76270994725343 2 0.8310991292137578 1.611302226059443 3 0.17646223151458715 0.5131770262902188 4 0.07121023114000154 0.2761191774142279 5 0.033304313129791874 0.16142272322611304 6 0.02301443187194383 0.13385841954318325 7 0.013486697598580793 0.09151617159278833 8 0.010129863623529467 0.0785575209143242 9 0.009124309987252627 0.07960433616903538 >10 0.041190519156880274 0.7041800098034605 >50 0.0020871188766777645 0.14712959486543473 >100 0.0012789956848770607 0.23952795525948067 >500 2.7828728894786198E-5 0.016779458401246495 >1k 0.0 0.0 >5k 2.7828727890351168E-5 0.18411543320754298 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6825 0.18427493550377255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.099997165000992E-5 2 0.0 0.0 0.0 0.0 8.099997165000992E-5 3 0.0 0.0 0.0 0.0 8.099997165000992E-5 4 0.0 0.0 0.0 0.0 8.099997165000992E-5 5 0.0 0.0 0.0 2.699999055000331E-5 8.099997165000992E-5 6 0.0 0.0 0.0 2.699999055000331E-5 1.3499995275001653E-4 7 0.0 0.0 0.0 2.699999055000331E-5 1.3499995275001653E-4 8 0.0 0.0 0.0 2.699999055000331E-5 1.3499995275001653E-4 9 0.0 0.0 0.0 5.399998110000662E-5 1.3499995275001653E-4 10-11 0.0 2.699999055000331E-5 0.0 5.399998110000662E-5 1.6199994330001985E-4 12-13 0.0 2.699999055000331E-5 0.0 8.099997165000992E-5 3.50999877150043E-4 14-15 0.0 4.049998582500496E-5 0.0 8.099997165000992E-5 4.184998535250513E-4 16-17 2.699999055000331E-5 5.399998110000662E-5 0.0 8.099997165000992E-5 4.859998299000595E-4 18-19 2.699999055000331E-5 5.399998110000662E-5 0.0 1.2149995747501489E-4 6.074997873750745E-4 20-21 2.699999055000331E-5 5.399998110000662E-5 0.0 1.3499995275001653E-4 6.479997732000794E-4 22-23 2.699999055000331E-5 5.399998110000662E-5 0.0 1.3499995275001653E-4 7.289997448500893E-4 24-25 2.699999055000331E-5 5.399998110000662E-5 0.0 1.8899993385002316E-4 7.559997354000926E-4 26-27 2.699999055000331E-5 5.399998110000662E-5 0.0 4.724998346250579E-4 8.639996976001058E-4 28-29 2.699999055000331E-5 5.399998110000662E-5 0.0 9.314996739751141E-4 8.909996881501091E-4 30-31 2.699999055000331E-5 5.399998110000662E-5 0.0 0.0032669988565504 9.314996739751141E-4 32-33 2.699999055000331E-5 5.399998110000662E-5 0.0 0.01019249643262625 0.0010394996361751273 34-35 2.699999055000331E-5 5.399998110000662E-5 0.0 0.01927799325270236 0.0010799996220001323 36-37 2.699999055000331E-5 5.399998110000662E-5 0.0 0.03434398797960421 0.0010799996220001323 38-39 2.699999055000331E-5 8.099997165000992E-5 0.0 0.05788797973920709 0.0010799996220001323 40-41 2.699999055000331E-5 8.099997165000992E-5 0.0 0.0832409708656602 0.0010799996220001323 42-43 2.699999055000331E-5 8.099997165000992E-5 0.0 0.11013296145346349 0.0011204996078251373 44-45 4.049998582500496E-5 9.449996692501158E-5 0.0 0.14326194985831756 0.0011474995983751406 46-47 5.399998110000662E-5 1.0799996220001324E-4 0.0 0.18554393505962274 0.0011609995936501423 48-49 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.23059341929230326 0.0012149995747501488 50-51 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.2829734009593097 0.0012149995747501488 52-53 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.35074337723981797 0.001241999565300152 54-55 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.42990734953242765 0.0012689995558501554 56-57 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.5177383187915885 0.0012959995464001588 58-59 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.6150057847479753 0.0012959995464001588 60-61 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.7196037481386881 0.0013229995369501621 62-63 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.8283867100646515 0.0014309994991501753 64-65 1.0799996220001324E-4 1.0799996220001324E-4 0.0 0.9431501698974405 0.0014579994897001786 66-67 1.3499995275001653E-4 1.0799996220001324E-4 0.0 1.069051125832106 0.001484999480250182 68-69 1.3499995275001653E-4 1.0799996220001324E-4 0.0 1.2100450764842234 0.0014984994755251836 70-71 1.6199994330001985E-4 1.0799996220001324E-4 0.0 1.3647010223546423 0.0015794994471751934 72-73 1.6199994330001985E-4 1.0799996220001324E-4 0.0 1.5260529658814619 0.0017009994046502082 74-75 1.6199994330001985E-4 1.0799996220001324E-4 0.0 1.689294908746782 0.0017684993810252166 76-77 1.6199994330001985E-4 1.0799996220001324E-4 0.0 1.868925845875954 0.0018089993668502216 78-79 1.6199994330001985E-4 1.0799996220001324E-4 0.0 2.075637773526779 0.001835999357400225 80-81 1.6199994330001985E-4 1.0799996220001324E-4 2.699999055000331E-5 2.2986306954792566 0.0018629993479502283 82-83 1.754999385750215E-4 1.0799996220001324E-4 2.699999055000331E-5 2.53014211445026 0.0019034993337752333 84-85 1.8899993385002316E-4 1.0799996220001324E-4 2.699999055000331E-5 2.765136532202214 0.001916999329050235 86-87 1.8899993385002316E-4 1.0799996220001324E-4 2.699999055000331E-5 3.0148999447850193 0.001984499305425243 88-89 2.1599992440002647E-4 1.0799996220001324E-4 2.699999055000331E-5 3.280687851759252 0.002051999281800251 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3505 0.0 40.358612 1 GGTATCA 2030 0.0 36.205517 1 ATCAACG 3875 0.0 33.373283 3 TCAACGC 4040 0.0 32.8673 4 CAACGCA 3945 0.0 32.824245 5 GTACATG 7550 0.0 31.60453 1 TATCAAC 4345 0.0 30.695866 2 AACGCAG 4340 0.0 30.24469 6 TACATGG 7760 0.0 29.964294 2 ACATGGG 7975 0.0 28.393526 3 CATGGGG 6315 0.0 24.212614 4 ACGCAGA 5355 0.0 24.019327 7 CGCAGAG 5420 0.0 23.50095 8 GAGTACA 5190 0.0 19.302061 1 GTATAAG 1100 0.0 19.220936 1 GTACTAG 685 0.0 19.10736 1 GCAGAGT 6480 0.0 18.456984 9 ATGGGGG 4070 0.0 18.282402 5 GTATAGG 1065 0.0 17.961885 1 AGTACAT 5265 0.0 17.780272 2 >>END_MODULE