FastQCFastQC Report
Sat 18 Jun 2016
SRR1930962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703705
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.042.7584151
GTATCAA48800.034.638311
CAACGCA54600.030.2459225
TCAACGC55850.029.6635554
ATCAACG57000.028.8156263
AACGCAG60300.027.3868546
TATCAAC62200.026.559962
GTACATG104900.026.162481
TACATGG107800.024.8369872
ACATGGG105600.024.1399973
ACGCAGA72100.022.5097717
CGCAGAG75850.021.459468
CATGGGG78400.021.13154
GAGTACA77100.020.1381661
ATGGGGG43550.017.8704685
AGTACAT81750.017.6532382
GCAGAGT91800.017.0072239
AGAGTAC81250.016.52887310-11
CTAACGC3201.616936E-716.3180983
TATACTG19500.015.5749115
Produced by FastQC (version 0.11.5)