##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930962_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3703705 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.218092423667652 31.0 31.0 33.0 30.0 34.0 2 31.336323492286777 31.0 31.0 34.0 30.0 34.0 3 31.427273500454273 31.0 31.0 34.0 28.0 34.0 4 35.29514391669963 37.0 35.0 37.0 33.0 37.0 5 35.071595874941444 35.0 35.0 37.0 32.0 37.0 6 35.10873220194373 37.0 35.0 37.0 32.0 37.0 7 34.98322922586977 36.0 35.0 37.0 32.0 37.0 8 34.95682296511196 36.0 35.0 37.0 32.0 37.0 9 36.468258946109366 38.0 35.0 39.0 32.0 39.0 10-11 36.386020349892874 38.0 35.0 39.0 32.0 39.0 12-13 36.278854687400866 38.0 35.0 39.0 32.0 39.0 14-15 37.3117008509047 39.0 36.0 41.0 32.0 41.0 16-17 37.26539397711211 39.0 36.0 40.5 32.0 41.0 18-19 37.222471822134864 39.0 36.0 40.0 31.0 41.0 20-21 37.170816385214266 39.0 36.0 40.0 31.0 41.0 22-23 37.039902881033996 39.0 36.0 40.0 31.0 41.0 24-25 36.91904673833364 39.0 36.0 40.0 31.0 41.0 26-27 36.64409867956546 38.0 35.0 40.0 30.0 41.0 28-29 36.458576479498234 38.0 35.0 40.0 30.0 41.0 30-31 36.24545367409121 38.0 35.0 40.0 30.0 41.0 32-33 36.04072557074605 38.0 34.5 40.0 29.0 41.0 34-35 36.29190553783306 38.0 35.0 40.0 30.0 41.0 36-37 36.37616454334241 38.0 35.0 40.0 30.0 41.0 38-39 36.36218934823373 38.0 35.0 40.0 30.0 41.0 40-41 36.28822651912071 38.0 35.0 40.0 30.0 41.0 42-43 36.176826313110794 38.0 35.0 40.0 30.0 41.0 44-45 36.03181287386549 38.0 35.0 40.0 29.0 41.0 46-47 35.86311949790817 38.0 34.0 40.0 29.0 41.0 48-49 35.70146245448814 38.0 34.0 40.0 28.0 41.0 50-51 35.43984024105592 38.0 34.0 40.0 27.5 41.0 52-53 35.220910277681405 38.0 33.5 40.0 27.0 41.0 54-55 35.03808497167026 38.0 33.0 40.0 27.0 41.0 56-57 34.777095098016716 37.0 33.0 40.0 26.0 41.0 58-59 34.52003250798862 37.0 33.0 40.0 26.0 41.0 60-61 34.200297674895815 36.5 32.5 39.5 26.0 41.0 62-63 33.880344411879456 36.0 32.0 39.0 26.0 41.0 64-65 33.55686981009556 36.0 32.0 39.0 25.0 40.0 66-67 33.20725111746211 35.0 31.0 39.0 24.5 40.0 68-69 32.8268502756024 35.0 31.0 38.0 23.5 40.0 70-71 32.412076150773345 35.0 31.0 37.5 23.0 40.0 72-73 31.989854078551076 34.5 30.5 37.0 22.0 39.0 74-75 31.455995280401652 34.0 30.0 36.5 21.0 39.0 76-77 30.08025490691078 32.5 28.5 35.0 20.0 37.5 78-79 30.86422582252096 34.0 30.0 36.0 20.5 37.5 80-81 30.873769509180672 34.0 30.0 35.0 20.5 37.0 82-83 30.605463988087607 34.0 30.0 35.0 20.0 37.0 84-85 30.29592691642558 34.0 30.0 35.0 19.5 36.0 86-87 29.961778273377604 34.0 29.0 35.0 18.0 36.0 88-89 29.623758506684524 34.0 29.0 35.0 17.5 35.5 90-91 29.282378996167353 34.0 29.0 35.0 13.0 35.0 92-93 28.93020232442919 33.0 29.0 35.0 7.0 35.0 94-95 28.502961898963335 33.0 29.0 35.0 2.0 35.0 96-97 28.02525714116 33.0 27.5 35.0 2.0 35.0 98-99 27.52374757168835 33.0 27.0 35.0 2.0 35.0 100-101 26.272742294540198 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4795271351687731 1101 2 0.5989335996145684 1101 3 0.5928866002613695 1101 4 0.3529774237344867 1101 5 0.49332476166593153 1101 6 0.5021459122406782 1101 7 0.511198020675053 1101 8 0.5234878500616418 1101 9 0.6365597065016857 1101 10-11 0.6627461102048997 1101 12-13 0.6355490266090627 1101 14-15 0.8249376407380069 1101 16-17 0.81719957890909 1101 18-19 0.8196527884278524 1101 20-21 0.7981457663152227 1101 22-23 0.8663358083694703 1101 24-25 0.8170007562141564 1101 26-27 0.8387046503887063 1101 28-29 0.6805212702332923 1101 30-31 0.8700153399331967 1101 32-33 0.7423248424062407 1101 34-35 0.5793098557405116 1101 36-37 0.7672811805327981 1101 38-39 0.8869692702391418 1101 40-41 0.8634828556941443 1101 42-43 0.795762605567333 1101 44-45 0.8309942194640314 1101 46-47 0.8500082452684481 1101 48-49 0.8375364325962664 1101 50-51 0.8084634150714933 1101 52-53 0.8965740809804217 1101 54-55 0.8291518418155448 1101 56-57 1.005468720738783 1101 58-59 0.9028715077564442 1101 60-61 0.8743890414508328 1101 62-63 0.7958937824817838 1101 64-65 0.909057618082187 1101 66-67 0.8833028532404228 1101 68-69 0.7890245735253387 1101 70-71 0.7237307641001749 1101 72-73 0.6275106915028879 1101 74-75 0.6030531755877 1101 76-77 0.733577095804236 1101 78-79 0.7705278689974868 1101 80-81 0.552261630788486 1101 82-83 0.7355107275992481 1101 84-85 0.8895242966939207 1101 86-87 0.8098889029574003 1101 88-89 0.6914448004307623 1101 90-91 0.6558667528344344 1101 92-93 0.8486726337890147 1101 94-95 0.8873775523148737 1101 96-97 0.7920337154029902 1101 98-99 0.7326317768683559 1101 100-101 0.9954271749912955 1102 1 0.4865528411962927 1102 2 0.5367854130562186 1102 3 0.5475519993913771 1102 4 0.37012325766797716 1102 5 0.5190231071464879 1102 6 0.43292208639253715 1102 7 0.5381058073832037 1102 8 0.559173328124615 1102 9 0.7392652250874221 1102 10-11 0.6736498062265639 1102 12-13 0.71051637793758 1102 14-15 0.8258731692143755 1102 16-17 0.7781584895957749 1102 18-19 0.8074973999566666 1102 20-21 0.8222479278493253 1102 22-23 0.788275715608826 1102 24-25 0.7926321239054133 1102 26-27 0.8787364828434079 1102 28-29 0.8262774230674736 1102 30-31 0.7993338469286613 1102 32-33 0.918517959298093 1102 34-35 0.8333743752769038 1102 36-37 0.7779076029105809 1102 38-39 0.932180478336015 1102 40-41 0.9027402424560762 1102 42-43 0.893976051058452 1102 44-45 0.9966530984622963 1102 46-47 1.0206246809937767 1102 48-49 0.9291990181789913 1102 50-51 0.9571015333557042 1102 52-53 0.9117367481848362 1102 54-55 0.8562027065252167 1102 56-57 0.8357155542977779 1102 58-59 0.8431508459486707 1102 60-61 0.8206903790861091 1102 62-63 0.7261535794838707 1102 64-65 0.7661793496140774 1102 66-67 0.810056038886529 1102 68-69 0.6838199911882654 1102 70-71 0.8681370384066369 1102 72-73 0.7265283505931457 1102 74-75 1.006913400863528 1102 76-77 1.0550786466161348 1102 78-79 0.9961995674005841 1102 80-81 0.9836655716751856 1102 82-83 1.0567722146502092 1102 84-85 0.9987617101509336 1102 86-87 0.9892350172853632 1102 88-89 1.1292781289628202 1102 90-91 1.0489279498883697 1102 92-93 1.152481485420008 1102 94-95 1.0139190017238384 1102 96-97 0.9707887927515308 1102 98-99 1.0949230414282205 1102 100-101 1.2034426639051503 1103 1 0.3816373849534429 1103 2 0.4757700706370862 1103 3 0.3877186416050371 1103 4 0.27930325122310506 1103 5 0.30754895263430626 1103 6 0.46293094976990545 1103 7 0.4420765736466308 1103 8 0.4600511575551849 1103 9 0.5559333991311917 1103 10-11 0.4614910345312211 1103 12-13 0.5189961780794761 1103 14-15 0.5868516311403695 1103 16-17 0.6502683619778722 1103 18-19 0.6147985986899371 1103 20-21 0.7006343845015195 1103 22-23 0.6483586950435196 1103 24-25 0.6308834927713107 1103 26-27 0.6907441483584478 1103 28-29 0.759597190006879 1103 30-31 0.8482053239371439 1103 32-33 0.7995076897053721 1103 34-35 0.6534546816295332 1103 36-37 0.7645587737406245 1103 38-39 0.7247999580698306 1103 40-41 0.7372031706935331 1103 42-43 0.7541482337204002 1103 44-45 0.7772597541016566 1103 46-47 0.7860885947208871 1103 48-49 0.7198054125396567 1103 50-51 0.6336295280283011 1103 52-53 0.5763667474010035 1103 54-55 0.6787211785941807 1103 56-57 0.7719661982827759 1103 58-59 0.7035482642471464 1103 60-61 0.6726510077360572 1103 62-63 0.5452447114838748 1103 64-65 0.535371140907337 1103 66-67 0.42085053381135396 1103 68-69 0.5541839189172393 1103 70-71 0.4861430933729807 1103 72-73 0.3230440986642087 1103 74-75 0.5285607429557295 1103 76-77 0.6757574821706704 1103 78-79 0.7618192434283948 1103 80-81 0.7587677215969002 1103 82-83 0.7547798305892819 1103 84-85 0.7663392345553675 1103 86-87 0.8365438801937692 1103 88-89 0.7856646847669246 1103 90-91 0.8217090070487814 1103 92-93 0.8357650418116549 1103 94-95 0.8811821542590117 1103 96-97 0.7826852473335926 1103 98-99 0.8230833580176089 1103 100-101 0.9398931525968095 1104 1 0.3813420541532153 1104 2 0.44749118850711156 1104 3 0.42785066174038633 1104 4 0.23353483946193165 1104 5 0.2961238130459094 1104 6 0.27144955858344844 1104 7 0.43441032784286904 1104 8 0.40718483008948425 1104 9 0.4799043496801261 1104 10-11 0.47515758293342714 1104 12-13 0.5103926368847951 1104 14-15 0.6277687885384751 1104 16-17 0.6157176645148823 1104 18-19 0.671978341507689 1104 20-21 0.6082302992903195 1104 22-23 0.5673532885738553 1104 24-25 0.5459564962987642 1104 26-27 0.6805452262167648 1104 28-29 0.6183366031905209 1104 30-31 0.5291916693473198 1104 32-33 0.6723824806778964 1104 34-35 0.676673992167558 1104 36-37 0.6264386038362204 1104 38-39 0.6300074923747871 1104 40-41 0.5550436616694299 1104 42-43 0.5547707077996691 1104 44-45 0.6422125441188129 1104 46-47 0.655670777618532 1104 48-49 0.5602086811314564 1104 50-51 0.6401517138462154 1104 52-53 0.578128920877127 1104 54-55 0.5507409968104824 1104 56-57 0.5667239444396799 1104 58-59 0.5380616984950848 1104 60-61 0.4589834022213637 1104 62-63 0.4047163072017739 1104 64-65 0.3808598473121805 1104 66-67 0.41019437748555987 1104 68-69 0.4308875052374148 1104 70-71 0.49539950063084603 1104 72-73 0.4436548189740499 1104 74-75 0.5946710381081424 1104 76-77 0.7149819382037101 1104 78-79 0.7428352870924257 1104 80-81 0.7477697215215819 1104 82-83 0.8151191174479884 1104 84-85 0.8968863497284332 1104 86-87 0.8681683251271295 1104 88-89 0.8345799882540099 1104 90-91 0.8546196935970904 1104 92-93 0.8473479884730608 1104 94-95 0.907112826179528 1104 96-97 0.9723843138371997 1104 98-99 1.1678621473198945 1104 100-101 1.507435802130324 1105 1 0.2172733719716149 1105 2 0.3576301668017017 1105 3 0.2926372943516853 1105 4 0.13520048237263893 1105 5 0.17913914748031345 1105 6 0.3135114556816987 1105 7 0.228782586372823 1105 8 0.2529484427297817 1105 9 0.43725838361545755 1105 10-11 0.38036264857615265 1105 12-13 0.37830937455809277 1105 14-15 0.48483115959378864 1105 16-17 0.49853738571859907 1105 18-19 0.45430469100427473 1105 20-21 0.3373621390221686 1105 22-23 0.4604087222537032 1105 24-25 0.4130206979888058 1105 26-27 0.4952199932248078 1105 28-29 0.49882650268946094 1105 30-31 0.473776243694104 1105 32-33 0.6169247118174397 1105 34-35 0.5785436582762102 1105 36-37 0.6272702126916343 1105 38-39 0.5384239594296858 1105 40-41 0.4899043580590501 1105 42-43 0.4607357997858088 1105 44-45 0.4970964402832365 1105 46-47 0.4639512313812446 1105 48-49 0.46954418158514244 1105 50-51 0.46712618835690733 1105 52-53 0.4631366678260278 1105 54-55 0.5380569733621883 1105 56-57 0.5944993281656181 1105 58-59 0.4525822697313515 1105 60-61 0.5005573256379918 1105 62-63 0.43546347346656233 1105 64-65 0.4359552704515366 1105 66-67 0.5069896127762306 1105 68-69 0.5991800119826607 1105 70-71 0.5891766007724257 1105 72-73 0.5355746854159484 1105 74-75 0.6946686455050575 1105 76-77 0.7476170117309451 1105 78-79 0.7460059751359687 1105 80-81 0.7481292639202692 1105 82-83 0.8005119822231457 1105 84-85 0.795708445802596 1105 86-87 0.6647644658518281 1105 88-89 0.6329955830192802 1105 90-91 0.5637962456695895 1105 92-93 0.6370650693512587 1105 94-95 0.6022774926865146 1105 96-97 0.41270526296132815 1105 98-99 0.7250612683544482 1105 100-101 1.0821568291737798 1106 1 0.05757144982134932 1106 2 0.1455106701590978 1106 3 0.07188812995125815 1106 4 -0.03487015028330376 1106 5 0.13313907982818307 1106 6 0.11079892427292037 1106 7 0.19307119547688956 1106 8 0.1651579932962406 1106 9 0.18094819475750512 1106 10-11 0.14226415855899432 1106 12-13 0.12412419662979346 1106 14-15 0.2067080084595645 1106 16-17 0.19827976512069512 1106 18-19 0.2226178042214002 1106 20-21 0.20154071767638726 1106 22-23 0.3515454950810337 1106 24-25 0.17506258614707093 1106 26-27 0.2566283446891262 1106 28-29 0.2910556021693722 1106 30-31 0.19009732071605612 1106 32-33 0.3924541376231545 1106 34-35 0.12218901686123473 1106 36-37 0.2711188148773118 1106 38-39 0.35037359177864147 1106 40-41 0.2486490025311383 1106 42-43 0.42032737237239814 1106 44-45 0.3433227915548045 1106 46-47 0.18284972701480484 1106 48-49 0.27065951634513397 1106 50-51 0.19706573543225403 1106 52-53 0.012570432239606077 1106 54-55 0.1406778233665591 1106 56-57 0.2838145034963375 1106 58-59 0.3002865967234669 1106 60-61 0.13444013044435366 1106 62-63 0.016301070692570363 1106 64-65 0.23249432136870496 1106 66-67 0.04457819079861025 1106 68-69 0.19014634611144032 1106 70-71 0.15594513921192998 1106 72-73 0.044959089301723765 1106 74-75 0.31773422531255235 1106 76-77 0.34478190869866054 1106 78-79 0.25369151270556145 1106 80-81 0.24843988932594385 1106 82-83 0.456983004453253 1106 84-85 0.42507253490173014 1106 86-87 0.3740963116892111 1106 88-89 0.543782875140554 1106 90-91 0.4021858951485697 1106 92-93 0.43850085822299434 1106 94-95 0.4346383478860574 1106 96-97 0.5063894576999068 1106 98-99 0.3656806938346655 1106 100-101 0.5853671462516665 1107 1 0.13575885123655596 1107 2 0.2174406845150969 1107 3 0.1674684846793184 1107 4 0.047432119008853135 1107 5 0.13644369494879527 1107 6 0.11442877206452096 1107 7 0.20247968982557296 1107 8 0.16353587125720281 1107 9 0.22674059422355697 1107 10-11 0.19632546345638247 1107 12-13 0.06859356252245874 1107 14-15 0.08129453520965768 1107 16-17 0.2106839937049827 1107 18-19 0.2869617090154577 1107 20-21 0.287876997439902 1107 22-23 0.291572957377106 1107 24-25 0.2132546692713433 1107 26-27 0.2984100178809683 1107 28-29 0.38658976154767544 1107 30-31 0.24313017288410066 1107 32-33 0.3530915650582145 1107 34-35 0.24140721496902273 1107 36-37 0.22833492642261177 1107 38-39 0.32442737982839986 1107 40-41 0.1714511017771514 1107 42-43 0.2502260534734049 1107 44-45 0.3150388026889388 1107 46-47 0.25683667791655296 1107 48-49 0.38968279867705746 1107 50-51 0.23892553036967001 1107 52-53 0.0749347761443957 1107 54-55 0.3212807814854699 1107 56-57 0.21183823161648974 1107 58-59 0.17253338807405783 1107 60-61 0.06513628752595224 1107 62-63 0.014522891684656258 1107 64-65 0.08871760593889633 1107 66-67 0.20339211431328863 1107 68-69 0.14942088638066764 1107 70-71 0.10705522652300914 1107 72-73 0.050474521843771214 1107 74-75 0.24041508508075538 1107 76-77 0.4056380645865154 1107 78-79 0.4426292497054227 1107 80-81 0.3595694868668353 1107 82-83 0.2679652493980349 1107 84-85 0.32273718277565777 1107 86-87 0.3612193424025918 1107 88-89 0.32036298303746946 1107 90-91 0.327645035268457 1107 92-93 0.46172036323018517 1107 94-95 0.4567926241889637 1107 96-97 0.5122718106410851 1107 98-99 0.4668171118733575 1107 100-101 0.874039804013762 1108 1 0.2579255860279197 1108 2 0.38170284074417893 1108 3 0.3294670289555981 1108 4 0.1483188434457574 1108 5 0.21194997178103137 1108 6 0.11567237320911516 1108 7 0.245234290536132 1108 8 0.25465968823372975 1108 9 0.31131941933227125 1108 10-11 0.28934796964928466 1108 12-13 0.3168691832661352 1108 14-15 0.4204671902168968 1108 16-17 0.4147901710342481 1108 18-19 0.4642681639017212 1108 20-21 0.5198029390143262 1108 22-23 0.5658125177787952 1108 24-25 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-0.6853822651226551 2316 74-75 -0.5523034145878363 2316 76-77 -1.0371757302410671 2316 78-79 -0.8098390379014546 2316 80-81 -0.8316564396894712 2316 82-83 -1.031321743550773 2316 84-85 -1.1192099150347232 2316 86-87 -0.9412079858135876 2316 88-89 -1.0460354872584396 2316 90-91 -0.6756832611668351 2316 92-93 -0.9628533569848869 2316 94-95 -0.5279722582995561 2316 96-97 -0.4539925038126462 2316 98-99 -0.4596741581952415 2316 100-101 -0.7192457848766516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 26.0 4 66.0 5 124.0 6 264.0 7 652.0 8 1500.0 9 2465.0 10 3842.0 11 5369.0 12 7148.0 13 9231.0 14 11138.0 15 13205.0 16 15303.0 17 17287.0 18 17674.0 19 18622.0 20 19704.0 21 21867.0 22 24605.0 23 29201.0 24 36164.0 25 45745.0 26 59282.0 27 75644.0 28 95731.0 29 117982.0 30 144354.0 31 172970.0 32 206541.0 33 249396.0 34 304287.0 35 377067.0 36 483716.0 37 595763.0 38 457062.0 39 62698.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24506515387647 20.449834904265536 13.52680513626373 24.77829480559427 2 16.124383775578476 21.207732364330724 38.77276770020105 23.895116159889753 3 18.95478203204906 25.195594955356615 30.053877715891574 25.795745296702748 4 13.360708472860475 17.042280302486017 37.37754518302522 32.219466041628294 5 13.450135447719989 37.87892083551249 35.28601433777499 13.384929378992522 6 32.36899267085256 37.22569697100606 17.191973982809106 13.213336375332268 7 27.91739622891132 31.835418857603397 22.254850210802427 17.992334702682854 8 25.79365797221971 36.350438277346605 20.017387993914202 17.838515756519485 9 26.115909910727208 15.34822977214162 19.722121422230284 38.81373889490089 10-11 25.098313715590198 25.881043441634795 28.73101394414512 20.289628898629886 12-13 25.396690611158284 23.18394688561859 28.61128248605113 22.808080017171996 14-15 22.53025011441246 25.002868748995937 25.432087598769343 27.03479353782226 16-17 22.91401448009493 28.038909686381608 26.16859334099233 22.878482492531127 18-19 22.84637950376717 27.405854407950958 27.38025841690955 22.367507671372312 20-21 23.483579280747254 26.581787696374302 27.26288945798869 22.671743564889752 22-23 23.26508813879295 26.414448886844088 27.107268831418494 23.213194142944467 24-25 23.251229585213444 26.582409209415047 27.042288659303914 23.124072546067595 26-27 23.17543654776763 26.744986172359162 26.932761282335655 23.146815997537555 28-29 23.174830274615665 26.911468480130768 26.8956599792613 23.01804126599227 30-31 23.42378879866849 26.68449503255265 26.874587463647188 23.01712870513167 32-33 23.321259226901606 26.74114578656446 26.741510372816453 23.196084613717492 34-35 23.401426253162974 26.713535147107255 26.909149777104908 22.975888822624867 36-37 23.477967741839677 26.564192993838837 26.885629790670528 23.072209473650958 38-39 23.27133872841422 26.652851587575338 26.942320244783403 23.13348943922704 40-41 23.33129299328146 26.547461126661915 27.033889175244507 23.087356704812116 42-43 23.292005466338573 26.52670132497415 26.83587726174349 23.34541594694379 44-45 23.272857718198185 26.56609404320659 26.89585916268417 23.265189075911053 46-47 23.377013329466628 26.585541466293982 26.73698291426702 23.30046228997237 48-49 23.315732547807876 26.612366112360508 26.777028348880343 23.294872990951273 50-51 23.316725279746088 26.616938537487687 26.78081440462921 23.285521778137017 52-53 23.411266027407326 26.489687134635627 26.81573263053706 23.283314207419988 54-55 23.226566776514506 26.423568343621834 26.936976252504387 23.41288862735927 56-57 23.22528459767483 26.603813681750697 26.807267468078454 23.36363425249602 58-59 23.21136162351808 26.592961800567473 26.871228569117655 23.324448006796793 60-61 23.22468760464 26.67590481563841 26.876219294512126 23.223188285209456 62-63 23.212160752764746 26.630612364586913 26.7582722150707 23.39895466757764 64-65 23.055270788946007 26.532259830033748 26.783076674006484 23.629392707013764 66-67 23.098267583306416 26.590886669216392 26.740302372190516 23.570543375286675 68-69 23.094485290341623 26.541888321953483 26.863026087257357 23.500600300447534 70-71 23.018624489281876 26.337636905427896 26.869853969345293 23.773884635944935 72-73 23.12454733597469 26.422528574321607 26.730107066820878 23.72281702288283 74-75 23.041622077190272 26.643978133699687 26.65515595084792 23.659243838262128 76-77 23.05191024817575 26.411741259475964 26.70306046962005 23.833288022728233 78-79 23.005227837987515 26.49371139234491 26.70768795415509 23.793372815512484 80-81 23.07134857368733 26.58568136089658 26.636954744421487 23.7060153209946 82-83 23.149791897572836 26.40929825674561 26.74808063817178 23.692829207509778 84-85 23.083992920602476 26.43282874850994 26.552830206509427 23.930348124378156 86-87 22.897841485755478 26.514179720037095 26.7367811421266 23.85119765208082 88-89 22.9588904616394 26.32164705453529 26.66006516452118 24.05939731930413 90-91 22.881944706961153 26.361534648065003 26.660673185241507 24.095847459732337 92-93 23.00174980525838 26.21109437835734 26.749916655043155 24.037239161341116 94-95 22.855250512097076 26.159915229422054 26.821722987107155 24.163111271373715 96-97 22.82121551257457 25.95580641546775 26.867326096435868 24.35565197552181 98-99 22.636697577155847 26.076064373377466 26.77130063004478 24.515937419421903 100-101 22.6658845669404 25.994713401850312 26.710699151255294 24.62870287995399 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 96.0 2 80.5 3 94.0 4 83.5 5 96.5 6 157.5 7 285.0 8 438.5 9 521.0 10 462.0 11 355.5 12 340.5 13 403.5 14 480.0 15 536.0 16 588.0 17 651.5 18 774.0 19 920.0 20 1079.0 21 1291.5 22 1587.5 23 2062.5 24 2722.5 25 3667.0 26 5210.5 27 7622.5 28 11338.5 29 17263.5 30 25024.0 31 34990.5 32 47878.5 33 59271.5 34 69769.0 35 80585.0 36 93172.0 37 106065.5 38 113520.0 39 115958.0 40 117038.0 41 119308.5 42 121965.5 43 126391.0 44 130752.5 45 136138.5 46 142597.0 47 146972.0 48 150610.5 49 151412.5 50 149168.5 51 147044.0 52 145290.5 53 143902.0 54 140609.0 55 130850.5 56 117862.5 57 104255.0 58 91982.0 59 81321.5 60 68854.5 61 56416.5 62 45278.0 63 34580.0 64 25614.5 65 18816.0 66 13503.0 67 10360.5 68 7865.5 69 5662.5 70 4142.5 71 2948.5 72 2097.0 73 1545.0 74 1115.0 75 770.5 76 490.0 77 275.5 78 154.0 79 86.5 80 61.0 81 35.0 82 15.0 83 13.0 84 10.5 85 5.5 86 5.5 87 4.0 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01895399336610232 2 0.002780999026650341 3 0.00359099874315044 4 0.03561298753545436 5 0.001673999414100205 6 0.0 7 0.0 8 0.0 9 2.6999990550003306E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6999990550003306E-5 24-25 4.5899983935005623E-4 26-27 0.0019574993148752397 28-29 4.049998582500496E-5 30-31 0.006574497698925805 32-33 0.023692491707627904 34-35 0.013648495223026674 36-37 5.939997921000728E-4 38-39 6.074997873750745E-4 40-41 0.001997999300700245 42-43 0.008423997051601032 44-45 0.00841049705632603 46-47 0.00792449722642597 48-49 0.009895496536576212 50-51 0.18912143380749816 52-53 0.05186698184655636 54-55 0.01900799334720233 56-57 0.010988996153851346 58-59 0.021154492595927592 60-61 0.0546884808590317 62-63 0.09822596562091203 64-65 0.10243796414671255 66-67 0.10691996257801309 68-69 0.10505696323006286 70-71 0.09512096670766165 72-73 0.07285947449918392 74-75 0.2399489160178794 76-77 0.2882788991023853 78-79 0.007073997524100866 80-81 7.829997259500959E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.749997637500827E-5 90-91 0.006398997760350784 92-93 0.019480493181827385 94-95 0.0016604994188252034 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3703705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.26221722345092 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87706626492978 41.93134918210729 2 13.121155547437288 14.502082961703348 3 4.366290956156519 7.238727578397322 4 2.0333625898628456 4.494725005401572 5 1.1265217833551537 3.112704574936091 6 0.7190828847900083 2.3842868748558694 7 0.5116391539796189 1.9792019847071045 8 0.3511441831993574 1.5524004902971307 9 0.2775441408296582 1.3803934139662162 >10 1.4961771994163935 14.726268762376252 >50 0.0904275693395354 3.382125850441352 >100 0.02796767638822791 2.6074617068049433 >500 0.0014236805824853365 0.5616091741611895 >1k 1.9636973311522651E-4 0.14666243984429655 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.699999055000331E-5 2 0.0 0.0 0.0 0.0 5.399998110000662E-5 3 0.0 0.0 0.0 0.0 5.399998110000662E-5 4 0.0 0.0 0.0 0.0 8.099997165000992E-5 5 0.0 0.0 0.0 0.0 1.3499995275001653E-4 6 0.0 0.0 0.0 0.0 2.4299991495002976E-4 7 0.0 0.0 0.0 0.0 2.4299991495002976E-4 8 0.0 0.0 0.0 0.0 2.4299991495002976E-4 9 0.0 0.0 0.0 0.0 2.6999990550003307E-4 10-11 0.0 0.0 0.0 0.0 2.969998960500364E-4 12-13 0.0 0.0 0.0 2.699999055000331E-5 4.994998251750611E-4 14-15 0.0 1.3499995275001655E-5 0.0 2.699999055000331E-5 5.939997921000728E-4 16-17 0.0 2.699999055000331E-5 0.0 2.699999055000331E-5 7.289997448500893E-4 18-19 0.0 2.699999055000331E-5 0.0 4.049998582500496E-5 8.504997023251042E-4 20-21 0.0 2.699999055000331E-5 0.0 5.399998110000662E-5 0.001093499617275134 22-23 0.0 4.049998582500496E-5 0.0 9.449996692501158E-5 0.001174499588925144 24-25 0.0 5.399998110000662E-5 0.0 2.8349990077503474E-4 0.0011879995842001456 26-27 0.0 5.399998110000662E-5 0.0 7.82999725950096E-4 0.0013229995369501621 28-29 0.0 5.399998110000662E-5 0.0 0.0016874994093752068 0.0013499995275001655 30-31 0.0 5.399998110000662E-5 0.0 0.006560997703650803 0.0013769995180501686 32-33 0.0 5.399998110000662E-5 0.0 0.020857492699877557 0.0014174995038751736 34-35 0.0 5.399998110000662E-5 0.0 0.0424709851351552 0.0014579994897001786 36-37 0.0 5.399998110000662E-5 0.0 0.07746297288795949 0.001484999480250182 38-39 0.0 5.399998110000662E-5 0.0 0.13120645407774106 0.001525499466075187 40-41 0.0 5.399998110000662E-5 0.0 0.18898643385474817 0.0015389994613501886 42-43 0.0 5.399998110000662E-5 0.0 0.24860241298915547 0.0015794994471751934 44-45 0.0 5.399998110000662E-5 0.0 0.3224743871339645 0.001673999414100205 46-47 0.0 5.399998110000662E-5 0.0 0.4123303556843755 0.001754999385750215 48-49 0.0 5.399998110000662E-5 0.0 0.5065603227038871 0.00187649934322523 50-51 5.399998110000662E-5 5.399998110000662E-5 0.0 0.6055422880601993 0.001916999329050235 52-53 5.399998110000662E-5 5.399998110000662E-5 0.0 0.7044027534590362 0.002186999234550268 54-55 5.399998110000662E-5 5.399998110000662E-5 0.0 0.8181402136509253 0.002335499182575286 56-57 5.399998110000662E-5 5.399998110000662E-5 0.0 0.9452291691697907 0.002429999149500298 58-59 5.399998110000662E-5 5.399998110000662E-5 0.0 1.086439119746308 0.002524499116425309 60-61 5.399998110000662E-5 5.399998110000662E-5 0.0 1.240609065786827 0.0025649991022503142 62-63 5.399998110000662E-5 5.399998110000662E-5 0.0 1.3996795101121715 0.002753999036100337 64-65 5.399998110000662E-5 5.399998110000662E-5 0.0 1.567862451248142 0.002807999017200344 66-67 5.399998110000662E-5 5.399998110000662E-5 0.0 1.7593598842240405 0.0028619989983003505 68-69 5.399998110000662E-5 5.399998110000662E-5 0.0 1.975400308609892 0.002888998988850354 70-71 5.399998110000662E-5 5.399998110000662E-5 0.0 2.2148632247978712 0.0029024989841253557 72-73 8.099997165000992E-5 5.399998110000662E-5 0.0 2.464086637569677 0.0031454988990753854 74-75 8.099997165000992E-5 5.399998110000662E-5 0.0 2.720303047893933 0.0032129988754503935 76-77 1.0799996220001322E-4 5.399998110000662E-5 0.0 3.006529947714518 0.003361498823475412 78-79 1.3499995275001653E-4 5.399998110000662E-5 0.0 3.329638834626408 0.003401998809300417 80-81 1.3499995275001653E-4 5.399998110000662E-5 0.0 3.6796667121166506 0.003469498785675425 82-83 1.3499995275001653E-4 5.399998110000662E-5 0.0 4.053765081182221 0.0038609986486504728 84-85 1.3499995275001653E-4 5.399998110000662E-5 0.0 4.442321945187319 0.0038609986486504728 86-87 1.3499995275001653E-4 5.399998110000662E-5 0.0 4.8602277989202705 0.003995998601400489 88-89 1.3499995275001653E-4 5.399998110000662E-5 0.0 5.311897140836001 0.004157998544700509 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2865 0.0 42.758415 1 GTATCAA 4880 0.0 34.63831 1 CAACGCA 5460 0.0 30.245922 5 TCAACGC 5585 0.0 29.663555 4 ATCAACG 5700 0.0 28.815626 3 AACGCAG 6030 0.0 27.386854 6 TATCAAC 6220 0.0 26.55996 2 GTACATG 10490 0.0 26.16248 1 TACATGG 10780 0.0 24.836987 2 ACATGGG 10560 0.0 24.139997 3 ACGCAGA 7210 0.0 22.509771 7 CGCAGAG 7585 0.0 21.45946 8 CATGGGG 7840 0.0 21.1315 4 GAGTACA 7710 0.0 20.138166 1 ATGGGGG 4355 0.0 17.870468 5 AGTACAT 8175 0.0 17.653238 2 GCAGAGT 9180 0.0 17.007223 9 AGAGTAC 8125 0.0 16.528873 10-11 CTAACGC 320 1.616936E-7 16.318098 3 TATACTG 1950 0.0 15.574911 5 >>END_MODULE