FastQCFastQC Report
Sat 18 Jun 2016
SRR1930961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537669
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83790.184654279543087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.051.3218651
GTATCAA62050.044.8539351
ATCAACG69950.038.4736673
TCAACGC71250.038.2577484
CAACGCA74000.036.0167545
TATCAAC76200.035.587852
AACGCAG81250.033.153686
ACGCAGA90750.029.0995087
CGCAGAG93400.027.9440848
GTACATG84150.026.0039221
GCAGAGT100500.023.6954739
TACATGG94300.022.7170352
ACATGGG92050.022.611863
AGAGTAC92050.019.21695710-11
CATGGGG64850.018.646974
CAGAGTA98950.018.56964710-11
GAGTACT56650.018.34252212-13
GTGGTAT17500.017.8631021
GAGTACA64850.017.4934621
GTATTAG15750.016.6466181
Produced by FastQC (version 0.11.5)