FastQCFastQC Report
Sat 18 Jun 2016
SRR1930961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537669
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.051.0383721
GTATCAA82150.040.6944161
TCAACGC99100.032.7652364
ATCAACG99150.032.6065143
CAACGCA99650.032.57475
AACGCAG106450.030.7603076
TATCAAC108000.030.5513362
ACGCAGA122400.026.4417537
CGCAGAG124150.025.9543578
GTACATG117050.023.1651121
GCAGAGT145300.021.555879
ACATGGG122300.020.6895353
TACATGG128900.020.4413342
AGAGTAC136350.018.9857110-11
CATGGGG82200.018.538054
GAGTACT81450.017.9451412-13
CAGAGTA143950.017.67015610-11
GTGGTAT24400.016.9316441
GAGTACA95050.016.4866141
GTACTTT92250.015.74136314-15
Produced by FastQC (version 0.11.5)