##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930961_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4537669 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28764879060152 31.0 31.0 34.0 30.0 34.0 2 31.42571725703219 31.0 31.0 34.0 30.0 34.0 3 31.50670399273283 31.0 31.0 34.0 30.0 34.0 4 35.3723347824621 37.0 35.0 37.0 33.0 37.0 5 35.16192212345149 37.0 35.0 37.0 32.0 37.0 6 35.19006675013096 37.0 35.0 37.0 32.0 37.0 7 35.081577126934555 37.0 35.0 37.0 32.0 37.0 8 35.059616512354694 37.0 35.0 37.0 32.0 37.0 9 36.55808257499611 39.0 35.0 39.0 32.0 39.0 10-11 36.49710721518031 38.0 35.0 39.0 32.0 39.0 12-13 36.41112496305922 38.0 35.0 39.0 32.0 39.0 14-15 37.44770872445743 39.0 36.0 41.0 32.0 41.0 16-17 37.42152876289566 39.0 36.0 41.0 32.0 41.0 18-19 37.42309663838415 39.0 36.0 41.0 32.0 41.0 20-21 37.39742596914847 39.0 36.0 41.0 32.0 41.0 22-23 37.28014361558765 39.0 36.0 40.0 31.0 41.0 24-25 37.17296468737583 39.0 36.0 40.0 31.0 41.0 26-27 36.924775473927255 39.0 36.0 40.0 31.0 41.0 28-29 36.75848965625302 39.0 36.0 40.0 30.0 41.0 30-31 36.54624411784994 38.0 35.0 40.0 30.0 41.0 32-33 36.3420660475676 38.0 35.0 40.0 30.0 41.0 34-35 36.53706881220292 38.0 35.0 40.0 30.0 41.0 36-37 36.597121561753404 38.5 35.0 40.0 30.0 41.0 38-39 36.578110148624766 39.0 35.0 40.0 30.0 41.0 40-41 36.507842352538276 39.0 35.0 40.0 30.0 41.0 42-43 36.401057679614794 38.5 35.0 40.0 30.0 41.0 44-45 36.25522895125228 38.0 35.0 40.0 30.0 41.0 46-47 36.09303223747699 38.0 35.0 40.0 29.5 41.0 48-49 35.94488646042715 38.0 35.0 40.0 29.0 41.0 50-51 35.718229117196515 38.0 34.5 40.0 28.5 41.0 52-53 35.53673031241371 38.0 34.0 40.0 28.0 41.0 54-55 35.40790172222786 38.0 34.0 40.0 27.5 41.0 56-57 35.19613142783222 38.0 34.0 40.0 27.0 41.0 58-59 34.992363149449645 38.0 33.5 40.0 27.0 41.0 60-61 34.72332082838126 37.5 33.0 40.0 26.0 41.0 62-63 34.46098646684013 37.0 33.0 40.0 26.0 41.0 64-65 34.19602674853543 37.0 33.0 40.0 26.0 41.0 66-67 33.90417657171557 36.0 32.0 39.0 26.0 41.0 68-69 33.58026544025137 36.0 32.0 39.0 25.0 40.5 70-71 33.21660196016941 35.5 31.5 39.0 24.5 40.0 72-73 32.83212482003425 35.0 31.0 38.0 23.5 40.0 74-75 32.327410505261625 35.0 31.0 37.5 22.0 40.0 76-77 30.903216497280873 33.5 29.5 36.0 21.5 39.0 78-79 31.69881198915126 34.0 30.5 36.0 22.0 39.0 80-81 31.69707199886109 34.0 31.0 36.0 23.0 39.0 82-83 31.399208271912297 34.0 31.0 36.0 22.0 37.5 84-85 31.04877934022953 34.0 30.5 35.5 21.5 37.0 86-87 30.69253222304227 34.0 30.0 35.0 20.0 37.0 88-89 30.33453486801263 34.0 30.0 35.0 19.5 36.0 90-91 29.99715812237517 34.0 30.0 35.0 18.0 36.0 92-93 29.646670460097464 34.0 29.0 35.0 15.5 36.0 94-95 29.24576825678559 34.0 29.0 35.0 7.5 35.0 96-97 28.811356227173025 34.0 29.0 35.0 2.0 35.0 98-99 28.356746051772397 34.0 29.0 35.0 2.0 35.0 100-101 27.172765686523192 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4841155268341133 1101 2 0.5400658319140312 1101 3 0.5627890906430615 1101 4 0.396854032041972 1101 5 0.46226159433712155 1101 6 0.4233285637514044 1101 7 0.4665737618138408 1101 8 0.4289950386100472 1101 9 0.597836153968629 1101 10-11 0.49402692118219704 1101 12-13 0.5559374392715384 1101 14-15 0.7363527614581713 1101 16-17 0.7302810983765866 1101 18-19 0.7592958234107954 1101 20-21 0.7235124899261365 1101 22-23 0.7383877343311909 1101 24-25 0.7089346651450938 1101 26-27 0.72906159170455 1101 28-29 0.6238631674203887 1101 30-31 0.7488794341340039 1101 32-33 0.7024936326123381 1101 34-35 0.6933239287330437 1101 36-37 0.7943964210316992 1101 38-39 0.9313936644436254 1101 40-41 0.8919737630271172 1101 42-43 0.8441361481967604 1101 44-45 0.8370749015026959 1101 46-47 0.9049768119982673 1101 48-49 0.8678450044071937 1101 50-51 0.8151447412583792 1101 52-53 0.8792288853779624 1101 54-55 0.8531870054138082 1101 56-57 0.9938292295884565 1101 58-59 0.8931683765639349 1101 60-61 0.770086214623106 1101 62-63 0.8217063421582367 1101 64-65 0.8727853131515886 1101 66-67 0.8981882261365897 1101 68-69 0.8561102775513874 1101 70-71 0.7394792790408289 1101 72-73 0.8316343539698394 1101 74-75 0.9060216639374303 1101 76-77 1.0380300279834636 1101 78-79 1.0201356569127213 1101 80-81 0.8742304418711733 1101 82-83 1.0537074787411562 1101 84-85 1.0457357579659075 1101 86-87 0.8139871706519735 1101 88-89 0.8318104265752524 1101 90-91 0.7158189404998652 1101 92-93 0.8496373328562363 1101 94-95 0.9293796370187621 1101 96-97 1.037087553304449 1101 98-99 0.9569466984081387 1101 100-101 1.110079337807182 1102 1 0.5055178888940652 1102 2 0.561398421002334 1102 3 0.5257410973117835 1102 4 0.30718112036301903 1102 5 0.4249419276492503 1102 6 0.4321045877606764 1102 7 0.5212438185709303 1102 8 0.5788056576005474 1102 9 0.6933651465535888 1102 10-11 0.6514342845920353 1102 12-13 0.6614481366948155 1102 14-15 0.7778374963538468 1102 16-17 0.7471298649029379 1102 18-19 0.8142286076500724 1102 20-21 0.7652650192084778 1102 22-23 0.8289472815623 1102 24-25 0.8758719303704368 1102 26-27 0.8993929798637623 1102 28-29 0.846491967647232 1102 30-31 0.8548261035340587 1102 32-33 0.8711512694895518 1102 34-35 0.8586344266996875 1102 36-37 0.8324369215507019 1102 38-39 0.8860087707429258 1102 40-41 0.9110677258370998 1102 42-43 0.8370285903729595 1102 44-45 0.9383098831008638 1102 46-47 0.9664677949447622 1102 48-49 0.8854535641689267 1102 50-51 0.9017165134511274 1102 52-53 0.8992270118354924 1102 54-55 0.7683106097604266 1102 56-57 0.7771997897194751 1102 58-59 0.9120092850729975 1102 60-61 0.8148723893148428 1102 62-63 0.6920203525321469 1102 64-65 0.7888037716178999 1102 66-67 0.8464900664362958 1102 68-69 0.8704644542710511 1102 70-71 0.7337725142336282 1102 72-73 0.6699294555290365 1102 74-75 0.7818648792699108 1102 76-77 0.9505116659095485 1102 78-79 0.9542331699499442 1102 80-81 0.9090625736289191 1102 82-83 0.9876520391452495 1102 84-85 1.038233466464419 1102 86-87 1.012414277436914 1102 88-89 0.9915653183442323 1102 90-91 0.902648702982777 1102 92-93 1.0498171764484105 1102 94-95 1.0186792705724734 1102 96-97 1.1185629650959008 1102 98-99 1.105894949652125 1102 100-101 1.3843878483835788 1103 1 0.4337855948495921 1103 2 0.5475663981029513 1103 3 0.5059649429825264 1103 4 0.32003522437644705 1103 5 0.3658689841851199 1103 6 0.4054531843281808 1103 7 0.4552325953382095 1103 8 0.45541388314012465 1103 9 0.5622443782890372 1103 10-11 0.568388363544166 1103 12-13 0.524547796102766 1103 14-15 0.7004862260783611 1103 16-17 0.6485606610066625 1103 18-19 0.6194934798676641 1103 20-21 0.7154211616411814 1103 22-23 0.791997346782054 1103 24-25 0.72096538937258 1103 26-27 0.7438204651591249 1103 28-29 0.665389580181504 1103 30-31 0.834767443498059 1103 32-33 0.8579689908441921 1103 34-35 0.7507180015876642 1103 36-37 0.8592779831229862 1103 38-39 0.895721343360286 1103 40-41 0.897684654892501 1103 42-43 0.7854485587896889 1103 44-45 0.8528533221747452 1103 46-47 0.8998372105032999 1103 48-49 0.8494477683671491 1103 50-51 0.8765310447747297 1103 52-53 0.8605510936258938 1103 54-55 0.8448054933310871 1103 56-57 0.8879165502905053 1103 58-59 0.8323907979901364 1103 60-61 0.8765641248245828 1103 62-63 0.7383248683962478 1103 64-65 0.6842717535496163 1103 66-67 0.6064438162702217 1103 68-69 0.7687801390180837 1103 70-71 0.8038635576893185 1103 72-73 0.7650631107339336 1103 74-75 0.8904457683721745 1103 76-77 1.0339202963550953 1103 78-79 1.0880963383014546 1103 80-81 1.029919534026039 1103 82-83 0.9882610210528107 1103 84-85 1.0916191190243012 1103 86-87 1.0776094988821363 1103 88-89 1.0434127584688788 1103 90-91 1.068930014503728 1103 92-93 1.0775176441585934 1103 94-95 1.1340440709275583 1103 96-97 1.0889274182161799 1103 98-99 1.2194930042313956 1103 100-101 1.4355138327187582 1104 1 0.39375876073016514 1104 2 0.4790386087783389 1104 3 0.4095160860101785 1104 4 0.26575682161480785 1104 5 0.3845564471579621 1104 6 0.3437862612672049 1104 7 0.3365088007460528 1104 8 0.45734297756632714 1104 9 0.5059054108271113 1104 10-11 0.5940042008570643 1104 12-13 0.6023429441404744 1104 14-15 0.6997313630420123 1104 16-17 0.6267923067199135 1104 18-19 0.6784828553238569 1104 20-21 0.5747242382638049 1104 22-23 0.6134458491665242 1104 24-25 0.6880835691475369 1104 26-27 0.7163117422973642 1104 28-29 0.6489602964599186 1104 30-31 0.6672726095706523 1104 32-33 0.710010448713156 1104 34-35 0.6236251148386671 1104 36-37 0.5519388050904084 1104 38-39 0.6434615957395735 1104 40-41 0.577088254450409 1104 42-43 0.5045471800429127 1104 44-45 0.6653203864162478 1104 46-47 0.7191150213759414 1104 48-49 0.7329976321508553 1104 50-51 0.6354254043181058 1104 52-53 0.6790456744321105 1104 54-55 0.6361910809159284 1104 56-57 0.6524661091286319 1104 58-59 0.5578476571925606 1104 60-61 0.6902382880244744 1104 62-63 0.6087790518240155 1104 64-65 0.6279267853697874 1104 66-67 0.6758481195486894 1104 68-69 0.5750891028904945 1104 70-71 0.5337773137350368 1104 72-73 0.4548297455422414 1104 74-75 0.6408362241757928 1104 76-77 0.8173683661423539 1104 78-79 0.8330210947541303 1104 80-81 0.687525457607439 1104 82-83 0.7927745428523316 1104 84-85 0.8051161383040579 1104 86-87 0.6957001495454342 1104 88-89 0.8460355086088036 1104 90-91 0.7620202287511759 1104 92-93 0.7742700010769319 1104 94-95 0.7920637712844396 1104 96-97 0.7753458570039484 1104 98-99 0.9274991620590072 1104 100-101 1.3207432156158418 1105 1 0.24259427334416728 1105 2 0.32826826418989086 1105 3 0.32978446426693964 1105 4 0.15425713183324774 1105 5 0.14687583195870246 1105 6 0.25303973789604584 1105 7 0.29020422310051686 1105 8 0.2493471859843197 1105 9 0.39993790212890445 1105 10-11 0.3675180955468136 1105 12-13 0.33667079105364195 1105 14-15 0.496690500048544 1105 16-17 0.4186668518910608 1105 18-19 0.5018866441596828 1105 20-21 0.42973586589473456 1105 22-23 0.4738206640363458 1105 24-25 0.48991690351589057 1105 26-27 0.48278506362187557 1105 28-29 0.5090846595453726 1105 30-31 0.4500546076434162 1105 32-33 0.5326971037797179 1105 34-35 0.5645420348309429 1105 36-37 0.5486413110484634 1105 38-39 0.3853352358225024 1105 40-41 0.45405212522248206 1105 42-43 0.2979944866655373 1105 44-45 0.4136638437449065 1105 46-47 0.37684877560393204 1105 48-49 0.4095606567469261 1105 50-51 0.3669523665438632 1105 52-53 0.32105820041265076 1105 54-55 0.4297307461759843 1105 56-57 0.3884538129656292 1105 58-59 0.33118141510255583 1105 60-61 0.343551776362645 1105 62-63 0.30398854539346587 1105 64-65 0.29287336600594216 1105 66-67 0.33100534526435155 1105 68-69 0.3222477094492646 1105 70-71 0.23957543660409186 1105 72-73 0.2553890636368834 1105 74-75 0.46718388402064903 1105 76-77 0.38167706438369464 1105 78-79 0.502602123470794 1105 80-81 0.41683678148548964 1105 82-83 0.47722297790634727 1105 84-85 0.5237448666674389 1105 86-87 0.6073409009598016 1105 88-89 0.6293363138202075 1105 90-91 0.608237724137318 1105 92-93 0.6290958543956471 1105 94-95 0.43929061841204486 1105 96-97 0.3954878840641918 1105 98-99 0.6704909274298352 1105 100-101 1.1033803995732399 1106 1 0.09251025118772205 1106 2 0.18328081343478786 1106 3 0.18870803612081488 1106 4 0.06615081517171006 1106 5 0.12499051420896024 1106 6 0.12101761385694942 1106 7 0.1317477177413977 1106 8 0.2025213912900199 1106 9 0.10513272847722988 1106 10-11 0.19293576956152947 1106 12-13 0.13621482036138133 1106 14-15 0.16792107496106468 1106 16-17 0.2100353861945834 1106 18-19 0.19811433847260673 1106 20-21 0.1383419512895827 1106 22-23 0.30297355143993343 1106 24-25 0.147185231562446 1106 26-27 0.28981065860035216 1106 28-29 0.17948750913705425 1106 30-31 0.2426967246208349 1106 32-33 0.25653016361767556 1106 34-35 0.2770391703902746 1106 36-37 0.35128046598525486 1106 38-39 0.320778478013402 1106 40-41 0.3286381871537003 1106 42-43 0.3970586085109815 1106 44-45 0.22594794420539444 1106 46-47 0.17051549837442082 1106 48-49 0.27127483370080085 1106 50-51 0.24513642018222725 1106 52-53 0.15924884517314553 1106 54-55 0.20372159478899476 1106 56-57 0.2877379678063221 1106 58-59 0.39353995439338263 1106 60-61 0.21317129246155275 1106 62-63 0.23728545002221324 1106 64-65 0.23750785389739093 1106 66-67 0.23184383889992688 1106 68-69 0.31646449100811225 1106 70-71 0.20044196319217633 1106 72-73 0.12909504529567783 1106 74-75 0.3760073264910062 1106 76-77 0.3441007521940911 1106 78-79 0.36298687246909367 1106 80-81 0.37429752151992446 1106 82-83 0.5232676711955229 1106 84-85 0.3826051601292235 1106 86-87 0.37095004276347154 1106 88-89 0.4222253824251858 1106 90-91 0.448872655688497 1106 92-93 0.5841069428727579 1106 94-95 0.584268740074954 1106 96-97 0.45951676176276735 1106 98-99 0.5959722073244045 1106 100-101 0.913674171609177 1107 1 0.14690774903392523 1107 2 0.1756726602765717 1107 3 0.2301192079398966 1107 4 0.04059044752234797 1107 5 0.11670230690963024 1107 6 0.17299910990519862 1107 7 0.18203376061087084 1107 8 0.12865642620131723 1107 9 0.22787933731778054 1107 10-11 0.2661091648919083 1107 12-13 0.11652121676790017 1107 14-15 0.18736842150977395 1107 16-17 0.21198468913276258 1107 18-19 0.3275737928702611 1107 20-21 0.3454099587284176 1107 22-23 0.20203562340063996 1107 24-25 0.19571242208593276 1107 26-27 0.2583988388696241 1107 28-29 0.3327707508010107 1107 30-31 0.3153063608893092 1107 32-33 0.38306123452451857 1107 34-35 0.31291803723329537 1107 36-37 0.31463448187326293 1107 38-39 0.3033792662384158 1107 40-41 0.201567906962282 1107 42-43 0.29744978516914955 1107 44-45 0.30965686382284474 1107 46-47 0.14701022548719322 1107 48-49 0.2250760756664434 1107 50-51 0.13874046922508398 1107 52-53 0.009882684991161739 1107 54-55 0.19348130055610824 1107 56-57 0.18237386676855039 1107 58-59 0.24311891305762146 1107 60-61 0.14818701950447632 1107 62-63 0.10605676656856389 1107 64-65 0.08957000988414165 1107 66-67 0.006467898289123752 1107 68-69 0.13397599903259305 1107 70-71 0.013899341235465101 1107 72-73 0.0513534932030737 1107 74-75 0.15450139253142225 1107 76-77 0.19916405518693736 1107 78-79 0.3261205730873442 1107 80-81 0.2927034260197736 1107 82-83 0.27671109382930936 1107 84-85 0.3204924518484056 1107 86-87 0.3671774759501112 1107 88-89 0.3715541358330512 1107 90-91 0.28746293154700453 1107 92-93 0.2752363237425577 1107 94-95 0.43043882295702574 1107 96-97 0.38745215586008896 1107 98-99 0.38676596489813875 1107 100-101 0.7431072539779855 1108 1 0.22942276206410028 1108 2 0.33953568672788137 1108 3 0.2864778289017593 1108 4 0.1218458257241295 1108 5 0.2613233225318936 1108 6 0.22906978760030228 1108 7 0.20747212339863097 1108 8 0.27758204479872717 1108 9 0.25781395335543067 1108 10-11 0.3136243548562092 1108 12-13 0.3544487380876049 1108 14-15 0.3977768177823222 1108 16-17 0.47564391810150397 1108 18-19 0.44007390770730836 1108 20-21 0.4194816415454312 1108 22-23 0.45308595707457044 1108 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-1.1258286430831141 2316 84-85 -0.9261042649878775 2316 86-87 -0.8611665363529042 2316 88-89 -1.0143837677635332 2316 90-91 -0.8020395067452561 2316 92-93 -0.9992518816545122 2316 94-95 -0.7874009829909525 2316 96-97 -0.7385103572041771 2316 98-99 -0.9375179808046745 2316 100-101 -1.1861092753558573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 24.0 4 64.0 5 127.0 6 239.0 7 597.0 8 1568.0 9 2670.0 10 4531.0 11 6802.0 12 9111.0 13 11495.0 14 13810.0 15 16903.0 16 19614.0 17 22030.0 18 20359.0 19 19178.0 20 19626.0 21 21676.0 22 24958.0 23 29341.0 24 37212.0 25 47113.0 26 61033.0 27 79263.0 28 101656.0 29 128412.0 30 158099.0 31 193180.0 32 233829.0 33 283328.0 34 349520.0 35 432841.0 36 559806.0 37 737372.0 38 732439.0 39 157827.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.35839116715766 24.157158113652972 16.353873571237273 22.130577147952092 2 13.849430800779722 23.546368441757217 41.1155445216159 21.488656235847152 3 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20.028653478250614 22-23 20.89219205510145 28.657813437809942 29.856292876525853 20.59370163056276 24-25 20.81204564752727 28.89079541144635 29.71239007774748 20.5847688632789 26-27 20.81154441567354 28.993962738430177 29.56391531547554 20.63057753042074 28-29 20.871355354123093 28.961692094383757 29.583608667582777 20.583343883910377 30-31 21.26736618092675 28.60926856027288 29.406553047942857 20.716812210857512 32-33 21.038833877131935 28.8167079089522 29.323672807380674 20.820785406535194 34-35 21.227829969206773 28.796381867725568 29.390646362372625 20.585141800695034 36-37 21.28503227560953 28.705083725295786 29.30828314384778 20.701600855246905 38-39 21.154036018723392 28.663479253532675 29.39703237806437 20.78545234967957 40-41 21.123359844974175 28.643756515744727 29.484768983550985 20.748114655730117 42-43 21.2309240167435 28.62813809807963 29.104359272317737 21.036578612859127 44-45 21.20077720178705 28.694876670127606 29.07313000696669 21.03121612111865 46-47 21.365877633241336 28.635990216654054 28.885620916833293 21.11251123327131 48-49 21.22823338675807 28.560712453253355 28.98619287917017 21.224861280818402 50-51 21.294157380307794 28.66890621996606 28.73662939315996 21.30030700656619 52-53 21.42803752577538 28.529043934120867 28.819595725652963 21.223322814450793 54-55 21.268663049316935 28.542295637998173 28.86304759767759 21.3259937150073 56-57 21.348808157320764 28.605271208346338 28.738625083091335 21.307295551241566 58-59 21.338977938143216 28.607839383572475 28.820063122675705 21.233119555608603 60-61 21.409733612442658 28.685759570183105 28.80066229480652 21.103844522567712 62-63 21.342570583674068 28.60899672298948 28.779619663827916 21.268813029508536 64-65 21.09693973787335 28.485927277051264 28.794486114355944 21.62264687071944 66-67 21.214149590946054 28.449877268373008 28.7921050227968 21.543868117884134 68-69 21.136818984612965 28.51787697277003 28.84953034524668 21.495773697370325 70-71 21.18330446728997 28.316777867937827 28.907826787857356 21.592090876914845 72-73 21.25848179787651 28.393270489518386 28.784136700293928 21.564111012311177 74-75 21.150979401230018 28.596892096026334 28.618001761900423 21.634126740843225 76-77 21.024064767168937 28.49234436492938 28.803737737262686 21.679853130638996 78-79 20.977109721310345 28.6191626329165 28.664784066262598 21.738943579510554 80-81 21.091898554498236 28.638781248839564 28.615255744272588 21.654064452389612 82-83 21.246007586714676 28.431734443389328 28.75367286595827 21.568585103937725 84-85 21.25771665729144 28.454453747437427 28.558505002845624 21.729324592425513 86-87 20.935286377212616 28.743656710086167 28.59856018585754 21.722496726843673 88-89 21.101130934829435 28.436009557344725 28.548258993287735 21.914600514538105 90-91 20.98191267432951 28.464685928356616 28.705002492094227 21.848398905219646 92-93 21.058093355603106 28.353522890897036 28.71357903188211 21.874804721617753 94-95 20.84560812229144 28.375062381730903 28.682968357738194 22.09636113823946 96-97 20.83468406355774 28.1987624042212 28.749948486767103 22.216605045453957 98-99 20.63438298386242 28.353070706567625 28.66448610509052 22.348060204479438 100-101 20.59976168380726 28.25457299772196 28.708881145804156 22.436784172666627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 34.0 1 28.5 2 35.0 3 62.0 4 62.5 5 61.0 6 109.0 7 223.5 8 367.0 9 446.0 10 457.0 11 469.0 12 534.5 13 670.0 14 813.0 15 970.0 16 1203.5 17 1449.5 18 1730.5 19 2038.0 20 2357.5 21 2833.5 22 3414.5 23 4274.5 24 5878.5 25 8594.5 26 13136.0 27 21295.0 28 35323.0 29 57871.0 30 87970.0 31 121417.5 32 155661.0 33 183264.5 34 204359.5 35 217657.5 36 228631.0 37 234181.5 38 219127.0 39 194403.5 40 170482.0 41 151337.0 42 139615.5 43 131949.5 44 127420.5 45 127486.5 46 127220.5 47 126486.0 48 126477.0 49 124671.0 50 121239.0 51 116959.0 52 114250.0 53 111835.0 54 107079.0 55 99046.0 56 89536.5 57 79194.5 58 68288.0 59 57666.0 60 47242.0 61 38569.5 62 31234.0 63 23776.5 64 17123.0 65 12500.0 66 9146.5 67 6960.5 68 5340.5 69 3950.0 70 3038.0 71 2193.5 72 1542.5 73 1167.5 74 831.0 75 566.5 76 362.5 77 202.5 78 115.5 79 62.5 80 39.0 81 29.5 82 16.0 83 9.5 84 5.0 85 3.5 86 2.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02124438781233272 2 0.003393812990766845 3 0.004054945391565582 4 0.0365826595108634 5 0.0016969064953834224 6 4.407549338658241E-5 7 0.0 8 0.0 9 2.2037746693291205E-5 10-11 0.0 12-13 0.0 14-15 2.2037746693291205E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.509436673322801E-5 24-25 6.611324007987361E-4 26-27 0.0021707180492891837 28-29 5.509436673322801E-5 30-31 0.007272456408786096 32-33 0.023426124734968547 34-35 0.013927855910160042 36-37 7.052078941853186E-4 38-39 6.390946541054449E-4 40-41 0.002258869036062348 42-43 0.008517589096957049 44-45 0.00806581528974458 46-47 0.008076834163091226 48-49 0.009806797278514585 50-51 0.1969843988179834 52-53 0.051414063035448376 54-55 0.0182913297554317 56-57 0.010864609119792562 58-59 0.020880764991893414 60-61 0.0557224425139868 62-63 0.10080065337511397 64-65 0.10626601455505018 66-67 0.11051929966685538 68-69 0.10852488359111252 70-71 0.09636004741641578 72-73 0.07168878999327628 74-75 0.24916978298769699 76-77 0.300782185743385 78-79 0.007228380915399515 80-81 8.925287410782937E-4 82-83 0.0 84-85 3.30566200399368E-5 86-87 0.0 88-89 1.5426422685303843E-4 90-91 0.00706309781519983 92-93 0.01931608497666974 94-95 0.001818114102196524 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4537669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.32132871614484 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13283285384955 36.8014132198144 2 14.008334572953363 14.098360176226452 3 5.024478512091176 7.585153045024391 4 2.4005176636340537 4.831889537625649 5 1.4015618499511522 3.5264227283699996 6 0.8622881432120026 2.603489106156322 7 0.5840645895798176 2.0573634342594356 8 0.43619040687766064 1.7559744677855784 9 0.31965762621119104 1.4477036836676311 >10 1.6707720460328712 14.871586395060143 >50 0.10151005087493206 3.5138498510791956 >100 0.05358789873205383 5.007120449003063 >500 0.003109050408330111 1.0516628090979991 >1k 0.0010947355919744021 0.8480110968299694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2037746693291202E-5 2 0.0 0.0 0.0 0.0 2.2037746693291202E-5 3 0.0 0.0 0.0 0.0 2.2037746693291202E-5 4 0.0 0.0 0.0 0.0 2.2037746693291202E-5 5 0.0 0.0 0.0 0.0 2.2037746693291202E-5 6 0.0 0.0 0.0 0.0 4.4075493386582404E-5 7 0.0 0.0 0.0 0.0 4.4075493386582404E-5 8 0.0 0.0 0.0 0.0 4.4075493386582404E-5 9 0.0 0.0 0.0 0.0 4.4075493386582404E-5 10-11 0.0 0.0 0.0 2.2037746693291202E-5 8.815098677316481E-5 12-13 0.0 0.0 0.0 6.61132400798736E-5 3.5260394709265923E-4 14-15 0.0 0.0 0.0 6.61132400798736E-5 4.7381155390576087E-4 16-17 0.0 0.0 0.0 6.61132400798736E-5 5.619625406789257E-4 18-19 0.0 0.0 0.0 6.61132400798736E-5 5.729814140255713E-4 20-21 0.0 2.2037746693291202E-5 0.0 7.71321134265192E-5 6.170569074121537E-4 22-23 0.0 3.30566200399368E-5 0.0 1.1018873346645601E-4 6.941890208386728E-4 24-25 0.0 6.61132400798736E-5 0.0 2.0935859358626644E-4 7.052078941853185E-4 26-27 0.0 6.61132400798736E-5 0.0 2.9750958035943125E-4 7.272456408786097E-4 28-29 0.0 6.61132400798736E-5 0.0 6.390946541054449E-4 7.492833875719009E-4 30-31 0.0 6.61132400798736E-5 0.0 0.0021927557959824746 7.603022609185466E-4 32-33 0.0 6.61132400798736E-5 0.0 0.006930871335040082 7.713211342651921E-4 34-35 0.0 6.61132400798736E-5 0.0 0.014765290284505106 7.713211342651921E-4 36-37 0.0 6.61132400798736E-5 0.0 0.02785571182032008 7.713211342651921E-4 38-39 0.0 6.61132400798736E-5 0.0 0.04980530752683812 7.823400076118377E-4 40-41 0.0 1.1018873346645601E-4 0.0 0.07848743484815662 8.264155009984201E-4 42-43 0.0 1.5426422685303843E-4 0.0 0.10973695965924354 8.925287410782936E-4 44-45 0.0 1.5426422685303843E-4 0.0 0.14965833779414056 9.366042344648761E-4 46-47 0.0 1.5426422685303843E-4 0.0 0.20042228730213685 9.806797278514585E-4 48-49 0.0 1.7630197354632962E-4 0.0 0.2535442757063153 0.001024755221238041 50-51 0.0 1.9833972023962083E-4 0.0 0.31118400218261844 0.0010578118412779777 52-53 0.0 1.9833972023962083E-4 0.0 0.37470780702603035 0.0010908684613179145 54-55 0.0 1.9833972023962083E-4 0.0 0.4493716928229009 0.0011459628280511426 56-57 0.0 1.9833972023962083E-4 0.0 0.5355172446469763 0.0011790194480910793 58-59 0.0 1.9833972023962083E-4 0.0 0.6335741985587754 0.001212076068131016 60-61 0.0 1.9833972023962083E-4 0.0 0.7424516860969806 0.0012230949414776618 62-63 0.0 1.9833972023962083E-4 0.0 0.8595382342784368 0.0013443025482907634 64-65 0.0 1.9833972023962083E-4 0.0 0.9903763363965066 0.0013663402949840545 66-67 0.0 1.9833972023962083E-4 0.0 1.138425918682037 0.0013773591683307002 68-69 0.0 1.9833972023962083E-4 0.0 1.3032241884544686 0.0013883780416773458 70-71 0.0 2.0935859358626644E-4 0.0 1.4811569552561017 0.0013883780416773458 72-73 0.0 2.2037746693291202E-4 0.0 1.6780972785806987 0.0014765290284505105 74-75 0.0 2.2037746693291202E-4 0.0 1.8949156494226442 0.001575698888570321 76-77 0.0 2.3139634027955763E-4 0.0 2.142509733521771 0.0015977366352636123 78-79 0.0 2.534340869728488E-4 0.0 2.415832886885315 0.0016418121286501945 80-81 0.0 2.644529603194944E-4 0.0 2.714521486692837 0.0016858876220367772 82-83 0.0 2.644529603194944E-4 0.0 3.0329559075375485 0.001851170722236461 84-85 0.0 2.644529603194944E-4 0.0 3.3836315517945446 0.001851170722236461 86-87 0.0 2.644529603194944E-4 0.0 3.771936648530336 0.0019062650889696891 88-89 0.0 2.644529603194944E-4 0.0 4.198124631831894 0.001961359455702917 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4345 0.0 51.038372 1 GTATCAA 8215 0.0 40.694416 1 TCAACGC 9910 0.0 32.765236 4 ATCAACG 9915 0.0 32.606514 3 CAACGCA 9965 0.0 32.5747 5 AACGCAG 10645 0.0 30.760307 6 TATCAAC 10800 0.0 30.551336 2 ACGCAGA 12240 0.0 26.441753 7 CGCAGAG 12415 0.0 25.954357 8 GTACATG 11705 0.0 23.165112 1 GCAGAGT 14530 0.0 21.55587 9 ACATGGG 12230 0.0 20.689535 3 TACATGG 12890 0.0 20.441334 2 AGAGTAC 13635 0.0 18.98571 10-11 CATGGGG 8220 0.0 18.53805 4 GAGTACT 8145 0.0 17.94514 12-13 CAGAGTA 14395 0.0 17.670156 10-11 GTGGTAT 2440 0.0 16.931644 1 GAGTACA 9505 0.0 16.486614 1 GTACTTT 9225 0.0 15.741363 14-15 >>END_MODULE