FastQCFastQC Report
Sat 18 Jun 2016
SRR1930960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615086
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10730.1744471504797703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.059.600561
GTATCAA10000.056.436311
TATCAAC13450.041.192772
ATCAACG13700.040.069053
TCAACGC14350.038.596594
CAACGCA13850.038.058015
AACGCAG14600.034.702956
ACGCAGA17650.029.4953147
GTACATG16600.029.1409531
ACTATAC700.0063029828.7782963
CGCAGAG19450.028.3853388
TACATGG17550.027.5514662
GTATATA750.00880288326.8744351
GCCTAAT1000.001171213525.194781
ACATGGG20150.024.9935553
CATGGGG16550.024.9473274
GCAGAGT20850.024.1947369
CCTAGTA1552.48043E-522.7466242
ATGGGGG9200.022.6021615
TATAGGC1352.550875E-422.3855652
Produced by FastQC (version 0.11.5)