##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930960_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615086 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.157921006168245 30.0 10.0 31.0 2.0 33.0 2 21.094911931014526 28.0 2.0 31.0 2.0 33.0 3 21.519057497650735 28.0 10.0 31.0 2.0 33.0 4 23.407432456599565 32.0 2.0 35.0 2.0 37.0 5 23.36135922456372 32.0 2.0 35.0 2.0 37.0 6 24.03279866555246 32.0 10.0 35.0 2.0 37.0 7 24.05107903610227 32.0 10.0 35.0 2.0 37.0 8 24.74378704766488 32.0 11.0 35.0 2.0 37.0 9 25.810862220892687 32.0 16.0 37.0 2.0 39.0 10-11 25.969267712157325 32.5 15.0 37.0 2.0 39.0 12-13 25.827555821462365 32.0 10.5 37.0 2.0 39.0 14-15 26.0095149296196 33.0 10.0 38.0 2.0 40.0 16-17 25.128787681722557 32.0 10.0 38.0 2.0 40.0 18-19 24.49106385123381 31.5 8.0 38.0 2.0 40.0 20-21 23.91975837525159 31.0 2.0 37.0 2.0 39.5 22-23 23.786333943546104 30.0 2.0 37.0 2.0 39.0 24-25 23.414970426899654 30.0 2.0 37.0 2.0 39.0 26-27 23.00967100535535 30.0 2.0 37.0 2.0 39.0 28-29 22.820884396653476 30.0 2.0 37.0 2.0 39.0 30-31 22.636679261111453 30.0 2.0 37.0 2.0 39.0 32-33 22.221686073167003 30.0 2.0 36.5 2.0 39.0 34-35 19.472360775566344 22.5 2.0 35.0 2.0 38.0 36-37 17.841399414065677 16.0 2.0 34.0 2.0 38.0 38-39 16.889346367824988 15.0 2.0 33.0 2.0 38.0 40-41 17.89580644007505 15.0 2.0 33.0 2.0 38.0 42-43 17.700979212662943 14.0 2.0 33.0 2.0 38.0 44-45 15.903089161515627 10.0 2.0 31.5 2.0 37.5 46-47 16.427629632279064 13.5 2.0 31.0 2.0 37.0 48-49 15.897942726708134 8.0 2.0 31.0 2.0 37.0 50-51 14.135011201685618 7.0 2.0 28.5 2.0 36.0 52-53 16.522895985276854 15.0 2.0 31.0 2.0 36.5 54-55 17.865438328949125 19.5 2.0 33.5 2.0 37.5 56-57 17.961524892454065 19.0 2.0 34.0 2.0 38.0 58-59 17.569599210516905 16.5 2.0 34.0 2.0 38.0 60-61 17.18076903067213 13.0 2.0 33.0 2.0 37.0 62-63 16.66267562584744 8.0 2.0 32.5 2.0 36.5 64-65 16.153733786820055 7.0 2.0 32.0 2.0 36.0 66-67 15.693060807756964 3.5 2.0 31.5 2.0 35.0 68-69 15.228307911414014 2.0 2.0 31.0 2.0 35.0 70-71 14.678061279235749 2.0 2.0 31.0 2.0 34.5 72-73 14.196170129055124 2.0 2.0 30.0 2.0 34.0 74-75 13.693808670657436 2.0 2.0 30.0 2.0 34.0 76-77 13.152702223754076 2.0 2.0 29.0 2.0 34.0 78-79 12.685869130495574 2.0 2.0 29.0 2.0 34.0 80-81 12.201234461522454 2.0 2.0 27.5 2.0 34.0 82-83 11.754683897861437 2.0 2.0 27.0 2.0 33.0 84-85 11.26651964115587 2.0 2.0 25.5 2.0 33.0 86-87 10.742960334002074 2.0 2.0 25.0 2.0 32.5 88-89 10.236375076005633 2.0 2.0 23.5 2.0 32.0 90-91 9.71090058951106 2.0 2.0 21.5 2.0 32.0 92-93 9.155481509902682 2.0 2.0 18.0 2.0 31.5 94-95 8.573637670179455 2.0 2.0 6.0 2.0 31.0 96-97 7.944891446074207 2.0 2.0 2.0 2.0 31.0 98-99 7.277026302013052 2.0 2.0 2.0 2.0 30.5 100-101 6.471733221045513 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.936689874190787 1101 2 7.779137848136898 1101 3 7.62119405053463 1101 4 9.092480388606003 1101 5 8.95808331299023 1101 6 8.422687896097898 1101 7 8.544497053986337 1101 8 7.765266081156344 1101 9 8.047162604016211 1101 10-11 7.304718349453168 1101 12-13 7.016749259363653 1101 14-15 7.381622291742513 1101 16-17 7.856188538548945 1101 18-19 8.47578867063456 1101 20-21 9.07739016807314 1101 22-23 8.478322168357113 1101 24-25 8.411971239587377 1101 26-27 8.687875019017405 1101 28-29 8.575187817771504 1101 30-31 8.400397398784154 1101 32-33 8.630856415695519 1101 34-35 10.65669880107626 1101 36-37 4.9491216209337665 1101 38-39 8.096367768528165 1101 40-41 9.405752650505981 1101 42-43 9.584559449215323 1101 44-45 4.112120774104961 1101 46-47 5.500374466746695 1101 48-49 8.401393478563543 1101 50-51 -3.1974621727982804 1101 52-53 5.054438916875455 1101 54-55 7.433299272836717 1101 56-57 8.070873355886544 1101 58-59 8.25511928740303 1101 60-61 7.7129090140796706 1101 62-63 8.244405178972524 1101 64-65 8.120496843769313 1101 66-67 8.134138755522674 1101 68-69 8.139315690556208 1101 70-71 7.769239038224988 1101 72-73 7.46144495113097 1101 74-75 7.378200708771541 1101 76-77 7.26427774021267 1101 78-79 7.3196624338054 1101 80-81 7.122504732999955 1101 82-83 6.986610080768289 1101 84-85 6.872387758310692 1101 86-87 6.711694221788912 1101 88-89 6.339390827244758 1101 90-91 5.98058243313279 1101 92-93 6.12051706502 1101 94-95 5.661994125473338 1101 96-97 5.290221300173254 1101 98-99 5.0197644511193555 1101 100-101 4.551741801895699 1102 1 6.5281174055867695 1102 2 7.351439613592213 1102 3 7.162463843654816 1102 4 8.937639036910316 1102 5 8.656085294830106 1102 6 8.20262277850199 1102 7 7.907441704029818 1102 8 7.091592194930129 1102 9 7.142467868025594 1102 10-11 6.592441559224852 1102 12-13 6.0479430145457584 1102 14-15 6.220504271155644 1102 16-17 7.281404417535374 1102 18-19 7.549705736702627 1102 20-21 7.931674380089564 1102 22-23 7.6103698336284005 1102 24-25 7.749885049775653 1102 26-27 8.121068814645223 1102 28-29 7.894046642014661 1102 30-31 7.3923032007215035 1102 32-33 7.52891552710323 1102 34-35 9.294684402402883 1102 36-37 0.996142288692468 1102 38-39 3.0036737807832132 1102 40-41 7.3457944107033555 1102 42-43 8.823644162758573 1102 44-45 0.8892497352568522 1102 46-47 1.305906428970399 1102 48-49 6.754823274919383 1102 50-51 -4.1790846525248675 1102 52-53 4.440604461173621 1102 54-55 6.848246996754288 1102 56-57 7.235184059844155 1102 58-59 7.573763243802556 1102 60-61 7.201945698340218 1102 62-63 7.283738633642994 1102 64-65 7.406354495086834 1102 66-67 7.637134266048669 1102 68-69 7.341845064254363 1102 70-71 7.020189512450958 1102 72-73 7.104524184280278 1102 74-75 6.571809275173287 1102 76-77 7.104514650969207 1102 78-79 6.9256731721418685 1102 80-81 6.987249217216016 1102 82-83 6.65738704289088 1102 84-85 6.746485313367417 1102 86-87 6.327556018791883 1102 88-89 5.603594855637647 1102 90-91 5.669107479139786 1102 92-93 5.561468853731602 1102 94-95 5.209388916065667 1102 96-97 5.2061852225983785 1102 98-99 5.137469055863637 1102 100-101 4.516463029827507 1103 1 6.711934977064949 1103 2 7.589777384167512 1103 3 7.178756238607935 1103 4 8.961195004036139 1103 5 8.899130400359294 1103 6 7.92347202802933 1103 7 8.125454977171 1103 8 7.4101282909288955 1103 9 7.428891836849143 1103 10-11 7.197001307652528 1103 12-13 6.694463638074712 1103 14-15 7.210814685169613 1103 16-17 7.721670459129694 1103 18-19 8.350825657989812 1103 20-21 8.33897460773937 1103 22-23 8.4014673757822 1103 24-25 8.502614918201783 1103 26-27 8.589752497170206 1103 28-29 8.71517755994498 1103 30-31 8.269324075051092 1103 32-33 8.26899925592987 1103 34-35 10.41828570656617 1103 36-37 -2.361663398694189 1103 38-39 -1.4615421006845484 1103 40-41 6.709981044958862 1103 42-43 9.082668162449895 1103 44-45 1.3761019943917834 1103 46-47 1.0371349662239382 1103 48-49 7.232072763671942 1103 50-51 -4.2256564007982025 1103 52-53 4.174403704913253 1103 54-55 7.283014716783612 1103 56-57 7.883214310258943 1103 58-59 7.88600520853613 1103 60-61 7.955220575833756 1103 62-63 7.753550919015531 1103 64-65 7.452241364709092 1103 66-67 7.700654349391648 1103 68-69 7.107245187725445 1103 70-71 6.8490084343940865 1103 72-73 6.767969027433418 1103 74-75 6.660086274826025 1103 76-77 6.329540695650305 1103 78-79 6.488339568715547 1103 80-81 6.316449163197417 1103 82-83 6.275547902558278 1103 84-85 6.470194654726372 1103 86-87 6.059266864954635 1103 88-89 5.804992586856537 1103 90-91 5.459992805373535 1103 92-93 5.228685352554768 1103 94-95 4.642854402040124 1103 96-97 4.148086291394922 1103 98-99 3.695794672254422 1103 100-101 3.1379469978793653 1104 1 6.355573687225057 1104 2 7.008825715755123 1104 3 6.759305767262731 1104 4 8.522544697287675 1104 5 8.34675871099823 1104 6 7.638205306497085 1104 7 7.605506974776862 1104 8 6.904255180050548 1104 9 6.686195817097538 1104 10-11 5.855537893018379 1104 12-13 5.661951106277616 1104 14-15 6.257818276371172 1104 16-17 6.964623661284417 1104 18-19 7.465921797448143 1104 20-21 7.253244022672785 1104 22-23 6.930673944688383 1104 24-25 7.2216454808233586 1104 26-27 7.796910584855379 1104 28-29 7.434399840357965 1104 30-31 6.656056268676711 1104 32-33 6.389309184105841 1104 34-35 8.711010717788678 1104 36-37 -2.9868784967040654 1104 38-39 -2.6505197304915225 1104 40-41 4.468411951737256 1104 42-43 6.948231906277517 1104 44-45 -0.07868533914106024 1104 46-47 -1.0809818974600365 1104 48-49 5.43368506353727 1104 50-51 -4.977035833687623 1104 52-53 2.459539497222096 1104 54-55 5.277684026973645 1104 56-57 5.946532617905209 1104 58-59 6.08192208718344 1104 60-61 5.881128663518929 1104 62-63 5.990327067451862 1104 64-65 5.9126773590230215 1104 66-67 5.602102052518987 1104 68-69 5.458729363993589 1104 70-71 5.802182532852861 1104 72-73 5.624657640331817 1104 74-75 5.653090913461369 1104 76-77 5.3696003619678265 1104 78-79 5.087560539836302 1104 80-81 4.697878723275229 1104 82-83 4.6767103670203465 1104 84-85 4.478494632281361 1104 86-87 4.781968121875179 1104 88-89 4.373052402807458 1104 90-91 4.298599157311287 1104 92-93 4.12020581492197 1104 94-95 3.827375762207712 1104 96-97 3.38362328825262 1104 98-99 3.0432013384223104 1104 100-101 2.7420871669651037 1105 1 6.421981794147207 1105 2 7.377047092871443 1105 3 6.922237258834016 1105 4 8.5851384004361 1105 5 8.745705579027632 1105 6 7.638980071553579 1105 7 7.739664743453499 1105 8 6.8772229644510325 1105 9 7.318273832967652 1105 10-11 6.666266674968867 1105 12-13 6.583547853635647 1105 14-15 6.585445621235319 1105 16-17 7.336610305157603 1105 18-19 8.104403623608231 1105 20-21 8.117989241526772 1105 22-23 7.981074811328231 1105 24-25 8.531760621423789 1105 26-27 8.064990721616265 1105 28-29 7.908636045426164 1105 30-31 7.958208927103595 1105 32-33 7.517818481286518 1105 34-35 9.428560860400296 1105 36-37 -3.0425541649674948 1105 38-39 -2.2359198657897252 1105 40-41 5.141187987499865 1105 42-43 6.980586917796149 1105 44-45 -2.7912920693125596 1105 46-47 -3.6923515175280492 1105 48-49 4.400125623744971 1105 50-51 -8.300271700510308 1105 52-53 1.0489300153776497 1105 54-55 5.756045456015222 1105 56-57 6.659658143557017 1105 58-59 6.918922032332819 1105 60-61 6.744739160099904 1105 62-63 7.082028168121038 1105 64-65 6.907522543138281 1105 66-67 6.5075304358882775 1105 68-69 6.2152303055959575 1105 70-71 6.481029847000663 1105 72-73 6.630531227928264 1105 74-75 6.6944437126715215 1105 76-77 6.358096586416579 1105 78-79 6.599222010930022 1105 80-81 6.564300314308978 1105 82-83 6.349925207770701 1105 84-85 6.085513912275511 1105 86-87 5.321778247300273 1105 88-89 5.345550528744491 1105 90-91 4.966134673089982 1105 92-93 4.838341030850589 1105 94-95 4.720511892313391 1105 96-97 4.542458123883584 1105 98-99 4.175547893803159 1105 100-101 3.5686963471311204 1106 1 5.561187307310256 1106 2 6.502652759898826 1106 3 5.991274610328286 1106 4 7.7385416060975665 1106 5 7.375861134943644 1106 6 6.067086729198483 1106 7 6.376007314053826 1106 8 5.405207040419228 1106 9 5.5297585079408265 1106 10-11 5.2503243370188315 1106 12-13 4.837678902485255 1106 14-15 5.089032699411842 1106 16-17 5.804017250834686 1106 18-19 5.725819731628409 1106 20-21 6.348710434253437 1106 22-23 6.126816082887196 1106 24-25 5.9300027677385785 1106 26-27 6.435974086193635 1106 28-29 6.239888116454022 1106 30-31 5.600861325788333 1106 32-33 5.529966580343633 1106 34-35 7.694507485233164 1106 36-37 -0.89404148158739 1106 38-39 -0.35051171978544815 1106 40-41 4.442533836986247 1106 42-43 5.857571918340696 1106 44-45 -3.3224287420890004 1106 46-47 -4.810073954609354 1106 48-49 2.9272859230389106 1106 50-51 -8.641025589408208 1106 52-53 -0.5635195558182033 1106 54-55 2.885790861519581 1106 56-57 4.055863441217305 1106 58-59 4.454549504531801 1106 60-61 4.383551902106028 1106 62-63 4.1113075778752055 1106 64-65 3.77595463312111 1106 66-67 4.210604710188534 1106 68-69 4.526345613475248 1106 70-71 4.342280828928558 1106 72-73 4.108603242925403 1106 74-75 4.258017026478296 1106 76-77 4.3220462190191355 1106 78-79 4.227155339474406 1106 80-81 3.669196861398648 1106 82-83 3.6593141763466956 1106 84-85 3.526908748087898 1106 86-87 3.2093981931233433 1106 88-89 3.2714778278460983 1106 90-91 2.9939461151278497 1106 92-93 2.961225106587065 1106 94-95 2.53553984974117 1106 96-97 2.1758832969230326 1106 98-99 2.0605574755656004 1106 100-101 2.034747710939169 1107 1 5.610887341373594 1107 2 6.856807212811471 1107 3 6.440977888519175 1107 4 7.4636991200762814 1107 5 8.273441710961663 1107 6 6.229684719229741 1107 7 6.321373888880785 1107 8 6.058632259803353 1107 9 5.859503218274995 1107 10-11 5.4751122521802635 1107 12-13 5.306936159482721 1107 14-15 5.443016835628125 1107 16-17 6.599728745937213 1107 18-19 6.445483083878095 1107 20-21 6.8001481620665025 1107 22-23 6.743443626920435 1107 24-25 6.858597203132934 1107 26-27 6.951797318317912 1107 28-29 7.207736495201278 1107 30-31 6.351762150491904 1107 32-33 6.047053863595366 1107 34-35 8.230659810925037 1107 36-37 -0.02981053678158574 1107 38-39 0.6606318583482071 1107 40-41 6.2064053788042095 1107 42-43 6.58328556847081 1107 44-45 -3.1953400453245546 1107 46-47 -4.076909238667481 1107 48-49 3.9683415157989455 1107 50-51 -8.414964354183471 1107 52-53 0.16662116980043962 1107 54-55 4.619613671907278 1107 56-57 5.674650647305143 1107 58-59 5.895683651523225 1107 60-61 5.826448305527194 1107 62-63 5.668235064672761 1107 64-65 5.959996306256723 1107 66-67 5.435566417897274 1107 68-69 5.699488176660422 1107 70-71 5.430804959444444 1107 72-73 5.32993152777834 1107 74-75 5.3166326182187476 1107 76-77 5.120465402008783 1107 78-79 5.12620851767665 1107 80-81 5.004330299316477 1107 82-83 4.874662838955112 1107 84-85 4.408602368047626 1107 86-87 4.567655308769536 1107 88-89 4.615415596210756 1107 90-91 4.406914283284884 1107 92-93 3.865327537597217 1107 94-95 3.8082180392399287 1107 96-97 3.6908839109900313 1107 98-99 3.3269721816592295 1107 100-101 2.9577518193949883 1108 1 5.623639711544193 1108 2 6.069534668325698 1108 3 6.119928500676654 1108 4 7.638433910115662 1108 5 7.678978009867606 1108 6 7.04206205825702 1108 7 7.089405325057744 1108 8 6.088337523789438 1108 9 4.87674459758534 1108 10-11 5.673677692492177 1108 12-13 5.432379268831838 1108 14-15 5.710219968755435 1108 16-17 5.587861501716755 1108 18-19 6.521782033528634 1108 20-21 7.019683438160232 1108 22-23 7.212481702634765 1108 24-25 6.692213193971337 1108 26-27 7.007191997071832 1108 28-29 6.642850206567168 1108 30-31 6.965634150352027 1108 32-33 6.792101529266901 1108 34-35 8.666441191371835 1108 36-37 -3.0882660029260656 1108 38-39 -2.7917013773662998 1108 40-41 4.05074013562405 1108 42-43 6.477177543765961 1108 44-45 -2.8609886350238742 1108 46-47 -4.341319307685449 1108 48-49 3.4142013118484424 1108 50-51 -8.485046659969354 1108 52-53 0.11779609254315204 1108 54-55 4.409874882409895 1108 56-57 5.665740379407023 1108 58-59 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3.6440167715796967 1109 20-21 3.7996132022736724 1109 22-23 5.660284070335091 1109 24-25 5.502415066928311 1109 26-27 4.150800089659516 1109 28-29 3.6183891234326175 1109 30-31 3.9631007993492915 1109 32-33 3.4043729313342226 1109 34-35 5.835793607663028 1109 36-37 -4.248454623828973 1109 38-39 -4.0288733890281065 1109 40-41 1.6153770293425787 1109 42-43 2.255576695634499 1109 44-45 -4.817354038328052 1109 46-47 -6.345394314311477 1109 48-49 -0.19214642205031218 1109 50-51 -8.939004606784444 1109 52-53 -2.3549930231031073 1109 54-55 0.4512058303734996 1109 56-57 1.8484773703072754 1109 58-59 2.3436460339683656 1109 60-61 2.7026318046867033 1109 62-63 2.868930171664722 1109 64-65 2.7182898867392105 1109 66-67 2.440350389547813 1109 68-69 2.727165247216055 1109 70-71 2.666530278603048 1109 72-73 2.7800530579223572 1109 74-75 2.8098167079920415 1109 76-77 3.094780517178471 1109 78-79 3.116639438579833 1109 80-81 2.826957622018762 1109 82-83 2.6525557107010567 1109 84-85 2.919269761623564 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50-51 -4.1860165215982175 1215 52-53 -7.400400659842013 1215 54-55 -9.059097669016687 1215 56-57 -13.099843297672832 1215 58-59 -13.441831027669688 1215 60-61 -11.61497069085697 1215 62-63 -10.81802451451597 1215 64-65 -10.54863946873504 1215 66-67 -10.665095712337918 1215 68-69 -10.498188913587022 1215 70-71 -10.028581833227332 1215 72-73 -9.644928178591387 1215 74-75 -9.393112741299637 1215 76-77 -8.935039353353474 1215 78-79 -8.78624779286777 1215 80-81 -8.362075120264755 1215 82-83 -8.265077275507782 1215 84-85 -7.9611786856979485 1215 86-87 -7.516024213698331 1215 88-89 -7.103244036821025 1215 90-91 -6.755082504698833 1215 92-93 -6.400194113342019 1215 94-95 -6.021726121806483 1215 96-97 -5.5191248617134 1215 98-99 -4.885626627295426 1215 100-101 -4.244752578560833 1216 1 -13.488615826750374 1216 2 -18.93629623546439 1216 3 -16.63119405470629 1216 4 -16.437309317310866 1216 5 -21.189826654855427 1216 6 -21.860270303259014 1216 7 -21.89601741546704 1216 8 -20.000142185090986 1216 9 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9.221418385879677 1301 34-35 11.94304468991411 1301 36-37 13.321404683530336 1301 38-39 13.599698039543323 1301 40-41 11.780149756615305 1301 42-43 11.565966306024112 1301 44-45 12.784485788511775 1301 46-47 12.001196594779433 1301 48-49 12.037561370157482 1301 50-51 13.389025696245858 1301 52-53 11.34414117603109 1301 54-55 10.287028341196013 1301 56-57 10.271475205805114 1301 58-59 9.919782397604727 1301 60-61 9.872328774383675 1301 62-63 9.785326275560823 1301 64-65 9.548971704921026 1301 66-67 9.116811428508854 1301 68-69 8.990909835960641 1301 70-71 8.896021620594386 1301 72-73 8.956459984329284 1301 74-75 8.882165192840525 1301 76-77 8.54542986782782 1301 78-79 8.302786624727021 1301 80-81 8.409858054919193 1301 82-83 8.319438827584174 1301 84-85 8.091131233485038 1301 86-87 7.199967805118765 1301 88-89 7.146997208002263 1301 90-91 6.944356601087023 1301 92-93 6.54274897298342 1301 94-95 6.277734092901408 1301 96-97 6.289140789044511 1301 98-99 5.307663248463491 1301 100-101 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8.096624179501287 1303 24-25 8.065707933482049 1303 26-27 8.06833726263147 1303 28-29 8.007693659476281 1303 30-31 8.002410040832448 1303 32-33 8.148119402254608 1303 34-35 10.275342826560074 1303 36-37 11.224954937624066 1303 38-39 11.622989965009157 1303 40-41 9.888457210031454 1303 42-43 9.728445131546412 1303 44-45 11.026227742210247 1303 46-47 10.355410315840981 1303 48-49 10.322887457114001 1303 50-51 11.183209773038628 1303 52-53 9.65561774294244 1303 54-55 8.377725687328137 1303 56-57 8.000336018905056 1303 58-59 7.963578275639737 1303 60-61 7.888774285394401 1303 62-63 7.48313118803965 1303 64-65 7.490141947721703 1303 66-67 6.751586979045271 1303 68-69 6.688538294458668 1303 70-71 6.636175488465646 1303 72-73 6.240833219789478 1303 74-75 6.218592352637252 1303 76-77 6.0080216238978394 1303 78-79 5.852970136867052 1303 80-81 5.436890804778029 1303 82-83 5.391502633343633 1303 84-85 5.307322650763412 1303 86-87 5.2251301427811025 1303 88-89 4.911678179204975 1303 90-91 4.683204709500348 1303 92-93 3.744718199409167 1303 94-95 3.3624320036862763 1303 96-97 3.4648183723307895 1303 98-99 3.3568233275148742 1303 100-101 2.8978353490587283 1304 1 6.218683443322618 1304 2 7.262128961386004 1304 3 6.7648631899629414 1304 4 8.670182492563264 1304 5 8.755055234908351 1304 6 7.857839932961458 1304 7 7.557525891619576 1304 8 6.555599524202652 1304 9 6.913752471601089 1304 10-11 6.479678473006857 1304 12-13 5.757684013901969 1304 14-15 6.2119233272641985 1304 16-17 6.666783014078732 1304 18-19 7.308482292934986 1304 20-21 7.718528735559282 1304 22-23 7.117722629151604 1304 24-25 7.315950022786101 1304 26-27 7.8752930507666505 1304 28-29 7.631017374446696 1304 30-31 7.42509674303815 1304 32-33 7.414000705135223 1304 34-35 9.44369148406484 1304 36-37 10.454832181229484 1304 38-39 11.061677792074256 1304 40-41 9.383791488898769 1304 42-43 8.707759945455546 1304 44-45 9.462829688706067 1304 46-47 8.670693731500979 1304 48-49 9.541568681743533 1304 50-51 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-15.063799752262096 2313 62-63 -14.505677978805497 2313 64-65 -14.036255567806247 2313 66-67 -13.565762463116764 2313 68-69 -13.117296612763045 2313 70-71 -12.54438926162684 2313 72-73 -12.061509115536369 2313 74-75 -11.56042885104662 2313 76-77 -11.07559565837012 2313 78-79 -10.596396089110867 2313 80-81 -10.08443213764686 2313 82-83 -9.633651185850667 2313 84-85 -9.11688488832419 2313 86-87 -8.615219163085444 2313 88-89 -8.118228945154925 2313 90-91 -7.584192436082705 2313 92-93 -7.0619588192243725 2313 94-95 -6.470642530475214 2313 96-97 -5.848846223942502 2313 98-99 -5.204512076211834 2313 100-101 -4.408065272059285 2314 1 -19.98131655667738 2314 2 -18.93629623546439 2314 3 -19.361121062005562 2314 4 -21.227455302712325 2314 5 -21.189826654855427 2314 6 -21.860270303259014 2314 7 -21.89601741546704 2314 8 -22.553061893120184 2314 9 -23.62325540241466 2314 10-11 -23.802998692347472 2314 12-13 -23.63759157664921 2314 14-15 -23.830596357775175 2314 16-17 -22.93400438749607 2314 18-19 -22.273407470844543 2314 20-21 -21.720841343180876 2314 22-23 -21.558655323604302 2314 24-25 -21.201372811859567 2314 26-27 -20.811321122461695 2314 28-29 -20.58236845232496 2314 30-31 -20.431596170347678 2314 32-33 -20.000172523211234 2314 34-35 -17.324812452943032 2314 36-37 -15.771939472313822 2314 38-39 -14.828873389028107 2314 40-41 -15.782350243384695 2314 42-43 -15.589877849820045 2314 44-45 -13.83402070499472 2314 46-47 -14.33403067794784 2314 48-49 -13.828510058413949 2314 50-51 -12.076883394663232 2314 52-53 -14.438326356436441 2314 54-55 -15.716218412050743 2314 56-57 -15.857583235753328 2314 58-59 -15.483626693304362 2314 60-61 -15.070853043798145 2314 62-63 -14.540160737426188 2314 64-65 -14.036255567806247 2314 66-67 -13.586922337724914 2314 68-69 -13.124349904299097 2314 70-71 -12.580439418366652 2314 72-73 -12.110098457229158 2314 74-75 -11.612152988977655 2314 76-77 -11.100674028276075 2314 78-79 -10.614421167480772 2314 80-81 -10.129102984041845 2314 82-83 -9.68380792566258 2314 84-85 -9.168609026255224 2314 86-87 -8.66772700007604 2314 88-89 -8.158197597192542 2314 90-91 -7.633565476835056 2314 92-93 -7.0619588192243725 2314 94-95 -6.470642530475214 2314 96-97 -5.848846223942502 2314 98-99 -5.204512076211834 2314 100-101 -4.408065272059285 2315 1 -19.98131655667738 2315 2 -18.93629623546439 2315 3 -19.361121062005562 2315 4 -17.562811903622794 2315 5 -21.189826654855427 2315 6 -21.860270303259014 2315 7 -21.89601741546704 2315 8 -22.553061893120184 2315 9 -20.234788027604342 2315 10-11 -19.160358328159308 2315 12-13 -16.47294817755968 2315 14-15 -16.34880576596941 2315 16-17 -19.918071155326118 2315 18-19 -22.273407470844543 2315 20-21 -21.720841343180876 2315 22-23 -21.558655323604302 2315 24-25 -21.201372811859567 2315 26-27 -20.811321122461695 2315 28-29 -20.58236845232496 2315 30-31 -20.431596170347678 2315 32-33 -20.000172523211234 2315 34-35 -17.324812452943032 2315 36-37 -15.771939472313822 2315 38-39 -14.828873389028107 2315 40-41 -15.782350243384695 2315 42-43 -15.589877849820045 2315 44-45 -13.83402070499472 2315 46-47 -14.33403067794784 2315 48-49 -13.828510058413949 2315 50-51 -12.076883394663232 2315 52-53 -14.438326356436441 2315 54-55 -15.716218412050743 2315 56-57 -15.857583235753328 2315 58-59 -15.483626693304362 2315 60-61 -15.070853043798145 2315 62-63 -14.540160737426188 2315 64-65 -14.036255567806247 2315 66-67 -13.586922337724914 2315 68-69 -13.124349904299097 2315 70-71 -12.580439418366652 2315 72-73 -12.110098457229158 2315 74-75 -11.612152988977655 2315 76-77 -11.100674028276075 2315 78-79 -10.614421167480772 2315 80-81 -10.129102984041845 2315 82-83 -9.68380792566258 2315 84-85 -9.168609026255224 2315 86-87 -8.66772700007604 2315 88-89 -8.158197597192542 2315 90-91 -7.633565476835056 2315 92-93 -7.0619588192243725 2315 94-95 -6.470642530475214 2315 96-97 -5.848846223942502 2315 98-99 -5.204512076211834 2315 100-101 -4.408065272059285 2316 1 -19.98131655667738 2316 2 -18.93629623546439 2316 3 -19.361121062005562 2316 4 -21.227455302712325 2316 5 -21.189826654855427 2316 6 -21.860270303259014 2316 7 -21.89601741546704 2316 8 -22.553061893120184 2316 9 -23.62325540241466 2316 10-11 -23.802998692347472 2316 12-13 -23.63759157664921 2316 14-15 -23.830596357775175 2316 16-17 -22.93400438749607 2316 18-19 -22.273407470844543 2316 20-21 -21.720841343180876 2316 22-23 -21.558655323604302 2316 24-25 -21.201372811859567 2316 26-27 -20.811321122461695 2316 28-29 -20.58236845232496 2316 30-31 -20.431596170347678 2316 32-33 -20.000172523211234 2316 34-35 -17.324812452943032 2316 36-37 -15.771939472313822 2316 38-39 -14.828873389028107 2316 40-41 -15.782350243384695 2316 42-43 -15.589877849820045 2316 44-45 -13.83402070499472 2316 46-47 -14.33403067794784 2316 48-49 -13.828510058413949 2316 50-51 -12.076883394663232 2316 52-53 -14.438326356436441 2316 54-55 -15.716218412050743 2316 56-57 -15.857583235753328 2316 58-59 -15.483626693304362 2316 60-61 -15.070853043798145 2316 62-63 -14.540160737426188 2316 64-65 -14.036255567806247 2316 66-67 -13.586922337724914 2316 68-69 -13.124349904299097 2316 70-71 -12.580439418366652 2316 72-73 -12.110098457229158 2316 74-75 -11.612152988977655 2316 76-77 -11.100674028276075 2316 78-79 -10.614421167480772 2316 80-81 -10.129102984041845 2316 82-83 -9.68380792566258 2316 84-85 -9.168609026255224 2316 86-87 -8.66772700007604 2316 88-89 -8.158197597192542 2316 90-91 -7.633565476835056 2316 92-93 -7.0619588192243725 2316 94-95 -6.470642530475214 2316 96-97 -5.848846223942502 2316 98-99 -5.204512076211834 2316 100-101 -4.408065272059285 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 116446.0 3 7332.0 4 14037.0 5 10180.0 6 9515.0 7 11855.0 8 15229.0 9 17742.0 10 18069.0 11 16202.0 12 13743.0 13 12204.0 14 11134.0 15 11489.0 16 12381.0 17 12991.0 18 13332.0 19 14221.0 20 14690.0 21 15284.0 22 15978.0 23 16654.0 24 17249.0 25 17870.0 26 18578.0 27 19139.0 28 19895.0 29 20309.0 30 19776.0 31 18985.0 32 17786.0 33 16047.0 34 13290.0 35 9405.0 36 4738.0 37 1216.0 38 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55824337996455 15.69943023193896 12.116889933590452 26.625436454506037 2 17.982855118080867 18.699815372373823 35.793929874656286 27.523399634889028 3 21.104364057368333 21.991194701068764 26.414124707605797 30.490316533957106 4 15.171611206456506 14.979403294212545 32.11237545872571 37.736610040605235 5 16.718205849032852 34.744052364368734 31.2111358200814 17.326605966517015 6 36.12975652200935 31.145993567333242 16.033693811277367 16.690556099380043 7 31.36202570438047 29.33498055890747 18.836024440230613 20.466969296481448 8 28.95477061247165 31.398176010369887 18.411925373825287 21.235128003333177 9 28.709640639042778 14.224033856926315 17.572502649264244 39.49382285476666 10-11 28.454855808181673 23.220195469786248 24.6127284230056 23.71222029902648 12-13 28.9771194215099 20.915880821326425 24.52950461389024 25.57749514327343 14-15 26.005998511356836 21.598683245031193 22.737574355265483 29.657743888346488 16-17 26.19050246348905 24.08536417154491 23.329489591551862 26.394643773414177 18-19 26.03086347980398 23.425327800576785 25.007195506191948 25.53661321342729 20-21 26.969989781226218 22.674653798297125 24.999185182202847 25.356171238273813 22-23 26.730932692730285 22.55067986149359 24.68269857463328 26.03568887114285 24-25 26.411382582198385 22.815934767299257 24.677193891756026 26.095488758746328 26-27 26.069499080860858 23.133305579852845 24.814418089032543 25.982777250253758 28-29 26.088291401688245 23.465309278254015 24.672993075147474 25.773406244910262 30-31 26.380510791829447 23.282894562672787 24.430531025327802 25.90606362016996 32-33 26.129931562651247 23.43052545429041 24.504416796180813 25.935126186877532 34-35 26.42095512349346 23.40868129323278 24.53598074851234 25.63438283476142 36-37 26.765529833168404 23.52485212873511 24.131453989709854 25.578164048386633 38-39 26.503290891117555 23.573700752807188 24.22374679993579 25.699261556139476 40-41 26.626705262435653 23.217721702761533 24.47980732610276 25.67576570870006 42-43 26.554831810420737 23.362380315369126 24.148815427763854 25.933972446446283 44-45 26.597581118934706 23.64507326459545 24.148581110420515 25.60876450604933 46-47 26.241216255987805 23.458167375345816 24.19506288417931 26.105553484487075 48-49 26.453294638924156 23.649884694115514 23.970564610162466 25.926256056797865 50-51 26.498126274893743 24.002620420861117 23.85793841597008 25.641314888275062 52-53 25.929362485556506 23.757997266864354 24.11703106945634 26.1956091781228 54-55 25.614710044548133 23.568075643966374 24.32182335159751 26.49539095988798 56-57 25.678594306827364 23.789640646020853 24.158375706254084 26.373389340897702 58-59 25.81339595870639 23.744105349125164 24.15997696711039 26.28252172505805 60-61 25.85353683537751 23.6491701504587 24.181129776470435 26.316163237693353 62-63 25.64393536082609 23.705357893380107 24.194578782495643 26.456127963298158 64-65 25.653542398826083 23.578487962566385 24.08262020178526 26.685349436822264 66-67 25.715537396731225 23.719819900928805 23.92559172160567 26.639050980734304 68-69 25.65193707443062 23.612866870157315 23.971073021836112 26.76412303357596 70-71 25.516264788690762 23.533231601427694 24.014764674149998 26.935738935731546 72-73 25.756106778154557 23.577928552231974 23.868052682799757 26.797911986813705 74-75 25.713189578740426 23.635068680234447 23.83753907605214 26.814202664972985 76-77 25.81017466442999 23.452025815408263 23.80506410238753 26.932735417774218 78-79 25.792590679310727 23.53086601970407 23.7452492527951 26.931294048190104 80-81 25.869665898468707 23.53905539328476 23.761396115218623 26.82988259302791 82-83 26.076175532952107 23.54267471442111 23.71245754295587 26.66869220967091 84-85 26.047014957162318 23.3911964551625 23.5972958741144 26.964492713560777 86-87 25.75303732996261 23.661183789654974 23.633417026833037 26.952361853549384 88-89 25.932580953127665 23.31985075384069 23.655239527171933 27.09232876585971 90-91 25.94434076759115 23.32845723464253 23.51731642474405 27.20988557302227 92-93 26.074470709844526 23.10661196517228 23.701412755060392 27.1175045699228 94-95 26.070297159674556 22.948335103506963 23.726140522716822 27.25522721410166 96-97 25.99062267450089 23.087760627102934 23.581280046019202 27.340336652376973 98-99 25.832697941329908 22.86508286849392 23.693950216884524 27.608268973291644 100-101 26.696394747531915 22.79410489417929 23.017202426322587 27.49229793196621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6588.0 1 7539.5 2 5711.0 3 2782.0 4 3518.0 5 4851.5 6 5498.5 7 5556.0 8 5172.0 9 4739.0 10 4384.5 11 3985.0 12 3669.5 13 3299.5 14 2790.0 15 2429.5 16 2089.0 17 1794.5 18 1559.0 19 1362.5 20 1317.0 21 1342.0 22 1399.5 23 1544.5 24 1760.0 25 2028.0 26 2309.0 27 2559.5 28 2806.5 29 3092.5 30 3434.0 31 3666.5 32 3938.5 33 4257.5 34 4521.0 35 4821.5 36 5128.5 37 5569.5 38 5992.0 39 6405.0 40 6949.5 41 7630.5 42 8469.5 43 9359.5 44 10410.5 45 11829.0 46 13298.5 47 14891.5 48 17015.0 49 19216.5 50 21215.0 51 23358.0 52 25357.0 53 26863.5 54 27688.5 55 27717.5 56 26815.0 57 25334.5 58 23910.0 59 21870.0 60 19472.5 61 17002.0 62 14287.5 63 11771.0 64 9430.5 65 7336.5 66 5717.0 67 4461.0 68 3413.5 69 2538.0 70 1841.5 71 1380.0 72 1037.0 73 741.5 74 515.0 75 365.0 76 260.0 77 166.0 78 99.0 79 55.0 80 28.5 81 20.5 82 15.5 83 7.0 84 2.5 85 2.0 86 1.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.51146668921094 2 21.540565059194975 3 17.430082947750396 4 22.60480648234556 5 22.505145621913034 6 16.29268102346664 7 15.119186585290512 8 12.203334167904977 9 3.0392497959634914 10-11 6.58794054815099 12-13 3.5920180267474797 14-15 7.496756551116429 16-17 11.599191007436358 18-19 14.705748464442369 20-21 15.200638609885447 22-23 13.068335159636213 24-25 15.620986333618388 26-27 13.106866356899685 28-29 13.156371629333133 30-31 14.024055172772588 32-33 12.495244567426344 34-35 19.265761210627456 36-37 24.178326282828742 38-39 23.030600598940637 40-41 18.252325691041577 42-43 18.20769778535034 44-45 23.619786501399805 46-47 12.995824974068668 48-49 21.569910549093947 50-51 21.453504062846495 52-53 11.427751566447618 54-55 13.032161356298143 56-57 12.567592174102483 58-59 13.603301001811129 60-61 13.004522944758945 62-63 12.964853695255623 64-65 14.023486146652663 66-67 12.4181821728994 68-69 13.447062687168948 70-71 11.997346712492236 72-73 11.37637663676299 74-75 11.431734749287092 76-77 11.466282763711092 78-79 11.8789892795479 80-81 11.888581434140917 82-83 10.37147650897598 84-85 10.309208793567079 86-87 10.416023125221514 88-89 11.872892571120136 90-91 9.309755058642207 92-93 11.371661848912185 94-95 11.44758619119928 96-97 10.97220551272505 98-99 10.871325310606972 100-101 10.948631573471026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 615086.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.94744882042565 #Duplication Level Percentage of deduplicated Percentage of total 1 99.47227030546996 98.42527375102036 2 0.43126300314258526 0.8534474786318805 3 0.054796208186833194 0.162658350153602 4 0.01840116557363056 0.07282993555331953 5 0.007539065377971762 0.03729856428203519 6 0.004516583369582508 0.026814264108297053 7 0.003238731336326416 0.022432494220098676 8 0.0019546407726417103 0.015472537425062629 9 8.264936966934085E-4 0.00736014984785779 >10 0.004199013116447422 0.08407662878261773 >50 4.974093688848639E-4 0.031498782328571 >100 3.315870590027245E-4 0.08481305781100808 >500 0.0 0.0 >1k 1.6579352944341404E-4 0.1760240058352322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1073 0.1744471504797703 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.8773667422116583E-4 2 0.0 0.0 0.0 0.0 4.8773667422116583E-4 3 0.0 0.0 0.0 0.0 4.8773667422116583E-4 4 0.0 0.0 0.0 0.0 4.8773667422116583E-4 5 0.0 0.0 0.0 0.0 6.503155656282211E-4 6 0.0 0.0 0.0 0.0 6.503155656282211E-4 7 0.0 0.0 0.0 0.0 6.503155656282211E-4 8 0.0 0.0 0.0 0.0 8.128944570352764E-4 9 0.0 0.0 0.0 0.0 8.128944570352764E-4 10-11 0.0 0.0 0.0 0.0 8.128944570352764E-4 12-13 0.0 0.0 0.0 0.0 0.002032236142588191 14-15 0.0 0.0 0.0 0.0 0.002276104479698774 16-17 0.0 0.0 0.0 0.0 0.0026012622625128843 18-19 0.0 0.0 0.0 8.128944570352763E-5 0.0027638411539199397 20-21 0.0 0.0 0.0 1.6257889140705527E-4 0.0028451305996234676 22-23 0.0 0.0 0.0 2.438683371105829E-4 0.0032515778281411055 24-25 0.0 0.0 0.0 4.8773667422116583E-4 0.0032515778281411055 26-27 0.0 0.0 0.0 0.0016257889140705527 0.0032515778281411055 28-29 0.0 0.0 0.0 0.0051212350793222415 0.0032515778281411055 30-31 0.0 0.0 0.0 0.01788367805477608 0.0032515778281411055 32-33 0.0 0.0 0.0 0.051700087467443576 0.0032515778281411055 34-35 0.0 0.0 0.0 0.0961654142672732 0.0032515778281411055 36-37 0.0 0.0 0.0 0.16046536581876356 0.0032515778281411055 38-39 0.0 0.0 0.0 0.25167212389812155 0.0032515778281411055 40-41 0.0 0.0 0.0 0.35052008987361116 0.0032515778281411055 42-43 8.128944570352763E-5 0.0 0.0 0.4520506075573172 0.0032515778281411055 44-45 1.6257889140705527E-4 0.0 0.0 0.5785369850720061 0.0033328672738446334 46-47 1.6257889140705527E-4 0.0 0.0 0.716241306093782 0.003414156719548161 48-49 1.6257889140705527E-4 0.0 0.0 0.8445160514139486 0.003414156719548161 50-51 1.6257889140705527E-4 0.0 0.0 0.9890486858748208 0.003414156719548161 52-53 1.6257889140705527E-4 0.0 0.0 1.1793472782667789 0.0036580250566587433 54-55 1.6257889140705527E-4 0.0 0.0 1.3907811265416545 0.0037393145023622712 56-57 1.6257889140705527E-4 0.0 0.0 1.6135954972150235 0.0039018933937693266 58-59 1.6257889140705527E-4 0.0 0.0 1.845676864698595 0.004064472285176382 60-61 1.6257889140705527E-4 0.0 0.0 2.0817414150216393 0.004227051176583437 62-63 1.6257889140705527E-4 0.0 0.0 2.3154485714192816 0.004552208959397548 64-65 1.6257889140705527E-4 0.0 0.0 2.562405907466598 0.004552208959397548 66-67 1.6257889140705527E-4 0.0 0.0 2.8135902946904983 0.004552208959397548 68-69 1.6257889140705527E-4 0.0 0.0 3.085097043340281 0.004552208959397548 70-71 1.6257889140705527E-4 0.0 0.0 3.360749553720943 0.004633498405101076 72-73 1.6257889140705527E-4 0.0 0.0 3.6455064820204006 0.004714787850804603 74-75 1.6257889140705527E-4 0.0 0.0 3.930425989211265 0.004714787850804603 76-77 1.6257889140705527E-4 0.0 0.0 4.219166100350195 0.004714787850804603 78-79 1.6257889140705527E-4 0.0 0.0 4.543592278152974 0.004958656187915186 80-81 1.6257889140705527E-4 0.0 0.0 4.863547536442058 0.005039945633618714 82-83 1.6257889140705527E-4 0.0 0.0 5.193663975444084 0.0052025245250257686 84-85 1.6257889140705527E-4 0.0 0.0 5.518659179366788 0.005527682307839879 86-87 1.6257889140705527E-4 0.0 0.0 5.866171559749368 0.005771550644950462 88-89 1.6257889140705527E-4 0.0 0.0 6.2144155451432805 0.006015418982061045 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 465 0.0 59.60056 1 GTATCAA 1000 0.0 56.43631 1 TATCAAC 1345 0.0 41.19277 2 ATCAACG 1370 0.0 40.06905 3 TCAACGC 1435 0.0 38.59659 4 CAACGCA 1385 0.0 38.05801 5 AACGCAG 1460 0.0 34.70295 6 ACGCAGA 1765 0.0 29.495314 7 GTACATG 1660 0.0 29.140953 1 ACTATAC 70 0.00630298 28.778296 3 CGCAGAG 1945 0.0 28.385338 8 TACATGG 1755 0.0 27.551466 2 GTATATA 75 0.008802883 26.874435 1 GCCTAAT 100 0.0011712135 25.19478 1 ACATGGG 2015 0.0 24.993555 3 CATGGGG 1655 0.0 24.947327 4 GCAGAGT 2085 0.0 24.194736 9 CCTAGTA 155 2.48043E-5 22.746624 2 ATGGGGG 920 0.0 22.602161 5 TATAGGC 135 2.550875E-4 22.385565 2 >>END_MODULE