FastQCFastQC Report
Sat 18 Jun 2016
SRR1930960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615086
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT27290.44367779464985385No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT18990.308737314781998TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT12610.20501198206429672RNA PCR Primer, Index 3 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.17721099163369025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.15851441912187889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.064.900971
GTATCAA13950.041.5183561
CCTAGTA1553.6379788E-1133.6911242
CTAATAC1151.04007995E-733.025293
TCTATAC1201.5111618E-731.6492393
TATCAAC18250.030.9555572
ATCAACG18950.029.8120783
CAACGCA19050.029.6483525
TCAACGC19250.029.5940914
ATTATAC650.00585772329.2146823
TAGGACG650.005864709629.2075565
CTAGTAC1503.3491233E-828.4843163
AACGCAG20000.028.0027476
TAGTACT1703.6561687E-927.9257984
GTACATG23750.027.584811
TACATGG23950.027.3544562
TAATACT2305.456968E-1226.833054
ACATGGG25550.024.8982283
GTGGTAT3250.024.832481
TATACAG1751.4952457E-724.4091725
Produced by FastQC (version 0.11.5)