##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930960_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615086 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196814754359554 31.0 31.0 33.0 30.0 34.0 2 31.29822984103036 31.0 31.0 34.0 28.0 34.0 3 31.384791720182218 31.0 31.0 34.0 28.0 34.0 4 35.265156417151424 35.0 35.0 37.0 33.0 37.0 5 35.01911927762947 35.0 35.0 37.0 32.0 37.0 6 35.06161739984327 36.0 35.0 37.0 32.0 37.0 7 34.92449348546382 36.0 35.0 37.0 32.0 37.0 8 34.89272231850506 35.0 35.0 37.0 32.0 37.0 9 36.395351219179105 38.0 35.0 39.0 32.0 39.0 10-11 36.310881730359654 38.0 35.0 39.0 32.0 39.0 12-13 36.176875266222936 38.0 35.0 39.0 31.0 39.0 14-15 37.16853334330484 39.0 36.0 40.0 31.0 41.0 16-17 37.09744328435373 39.0 36.0 40.0 31.0 41.0 18-19 37.0517293516679 38.5 36.0 40.0 31.0 41.0 20-21 36.99431217748412 39.0 36.0 40.0 31.0 41.0 22-23 36.83461824850509 38.5 35.5 40.0 30.5 41.0 24-25 36.65980529551965 38.0 35.0 40.0 30.0 41.0 26-27 36.33813808150405 38.0 35.0 40.0 30.0 41.0 28-29 36.104180553613645 38.0 34.5 40.0 29.5 41.0 30-31 35.805676767151255 38.0 34.0 40.0 29.0 41.0 32-33 35.508591481516405 38.0 34.0 40.0 27.5 41.0 34-35 35.72595945932764 38.0 34.0 40.0 29.0 41.0 36-37 35.755626042537145 38.0 34.0 40.0 29.0 41.0 38-39 35.669068227857565 38.0 34.0 40.0 28.0 41.0 40-41 35.520579236074305 38.0 34.0 40.0 27.5 41.0 42-43 35.31580868366375 38.0 34.0 40.0 27.0 41.0 44-45 35.04139177285778 38.0 33.0 40.0 26.5 41.0 46-47 34.71356850911905 38.0 33.0 40.0 26.0 41.0 48-49 34.42141098968274 37.0 33.0 40.0 25.5 41.0 50-51 34.02133116344706 37.0 32.5 40.0 24.0 41.0 52-53 33.70506075573172 36.5 32.0 40.0 23.5 41.0 54-55 33.4105100750139 36.0 32.0 39.0 23.0 41.0 56-57 32.996384245455104 35.5 31.0 39.0 22.5 40.5 58-59 32.60161668449615 35.0 31.0 39.0 22.0 40.0 60-61 32.12533125449124 35.0 31.0 38.0 20.5 40.0 62-63 31.665962971031693 35.0 30.0 37.5 19.5 40.0 64-65 31.224789704203964 34.5 30.0 37.0 18.0 39.5 66-67 30.76550108440121 34.0 29.5 36.5 16.0 39.0 68-69 30.312850560734596 34.0 29.0 36.0 12.5 39.0 70-71 29.886866226836574 34.0 29.0 35.5 9.5 38.0 72-73 29.480301128622663 34.0 29.0 35.0 7.5 37.0 74-75 28.987242434391288 33.0 28.0 35.0 7.0 36.5 76-77 27.722342078993833 31.5 26.0 34.0 5.5 35.0 78-79 28.499916271870923 33.0 27.5 35.0 3.5 36.0 80-81 28.553397411093734 33.0 28.0 35.0 2.0 35.0 82-83 28.353176628959204 33.0 27.0 35.0 2.0 35.0 84-85 28.104715438166373 33.0 27.0 35.0 2.0 35.0 86-87 27.816533785519425 33.0 27.0 35.0 2.0 35.0 88-89 27.523697499211494 33.0 26.5 35.0 2.0 35.0 90-91 27.204406375693807 33.0 25.5 35.0 2.0 35.0 92-93 26.86235989764033 33.0 25.0 35.0 2.0 35.0 94-95 26.42499260266044 32.0 24.5 34.5 2.0 35.0 96-97 25.944634571425784 32.0 24.0 34.0 2.0 35.0 98-99 25.41750828339452 32.0 23.0 34.0 2.0 35.0 100-101 24.150257850121772 31.0 17.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49592583750480657 1101 2 0.5130353893925808 1101 3 0.5829259145988779 1101 4 0.4134316390831927 1101 5 0.6547032063739877 1101 6 0.5630826403215323 1101 7 0.4577822490998784 1101 8 0.4217140280817162 1101 9 0.5844507420388112 1101 10-11 0.4881812571921813 1101 12-13 0.6936026440610163 1101 14-15 0.7120041328556894 1101 16-17 0.7635919691444144 1101 18-19 0.7735765656732312 1101 20-21 0.7349452219160142 1101 22-23 0.7854158389403949 1101 24-25 0.5299651298471559 1101 26-27 0.5484544450916502 1101 28-29 0.8667322429151625 1101 30-31 0.7194698136851869 1101 32-33 0.7171541762413227 1101 34-35 0.8438615733302797 1101 36-37 0.7553013706562623 1101 38-39 1.0039487693228821 1101 40-41 0.756462718344828 1101 42-43 0.4628100046933028 1101 44-45 0.6640325902963653 1101 46-47 0.7121892932527842 1101 48-49 0.8765525728823107 1101 50-51 0.5164435895474853 1101 52-53 0.646141560750273 1101 54-55 0.19247688644078664 1101 56-57 0.25321529605942317 1101 58-59 0.5485418639019883 1101 60-61 0.42389059008677776 1101 62-63 0.355958810031769 1101 64-65 0.39326192358253564 1101 66-67 0.1381531210062512 1101 68-69 0.42536425620071583 1101 70-71 -0.03481631912123362 1101 72-73 -0.07006597986575613 1101 74-75 0.1924691773573386 1101 76-77 0.39780922824685305 1101 78-79 0.17280869307348468 1101 80-81 -0.018327246147610055 1101 82-83 0.15938171536710755 1101 84-85 0.09910186407965682 1101 86-87 0.018413351668836953 1101 88-89 -0.2914964220790708 1101 90-91 -0.12048034286524256 1101 92-93 -0.07382273364022751 1101 94-95 -0.11144192129925656 1101 96-97 0.0166418899216616 1101 98-99 0.23992483122411556 1101 100-101 0.529491604333689 1102 1 0.3201892199796781 1102 2 0.4178716853743012 1102 3 0.4357641924204714 1102 4 0.19866996543964177 1102 5 0.47202509357089895 1102 6 0.26143852213915864 1102 7 0.4338484991757099 1102 8 0.4057683062268751 1102 9 0.45933698847453286 1102 10-11 0.38164978031701935 1102 12-13 0.49561835722002456 1102 14-15 0.5999138984727921 1102 16-17 0.5881763894562511 1102 18-19 0.8047551537134368 1102 20-21 0.5831903835977457 1102 22-23 0.400360023858191 1102 24-25 0.6846042529841228 1102 26-27 0.886800518824181 1102 28-29 1.0677524859938927 1102 30-31 0.6953363630592833 1102 32-33 0.6763191745223764 1102 34-35 0.6363573770876911 1102 36-37 0.7065415372926012 1102 38-39 0.8773183015682307 1102 40-41 0.8669047899741926 1102 42-43 0.6801703153163672 1102 44-45 0.7059495148044519 1102 46-47 0.702846333840057 1102 48-49 0.6166661242180282 1102 50-51 0.7824575635408806 1102 52-53 0.8308874605662453 1102 54-55 0.7721852728591188 1102 56-57 0.22657421130543298 1102 58-59 0.29030357799124573 1102 60-61 0.3532673216688096 1102 62-63 0.33923703608285294 1102 64-65 -0.048273800092360375 1102 66-67 0.34360975309183317 1102 68-69 0.31670685160204215 1102 70-71 0.21084335043730462 1102 72-73 0.08723244881834447 1102 74-75 0.2702235303270655 1102 76-77 0.3599823763388237 1102 78-79 0.14959032667578143 1102 80-81 0.15130712461822782 1102 82-83 0.40664404625347217 1102 84-85 0.452435601870107 1102 86-87 0.3359854967071598 1102 88-89 0.42361409582827037 1102 90-91 0.5462823823668153 1102 92-93 0.508459011187778 1102 94-95 0.5939388750768089 1102 96-97 0.15244595067112243 1102 98-99 0.1006626621205946 1102 100-101 0.3044567199073107 1103 1 0.36566130394001206 1103 2 0.5288413192053767 1103 3 0.50910408515497 1103 4 0.3052312958405352 1103 5 0.365319070497236 1103 6 0.3208203950411175 1103 7 0.3915403616597928 1103 8 0.5691456261327303 1103 9 0.4646906173667702 1103 10-11 0.5363166013225396 1103 12-13 0.5309667771760402 1103 14-15 0.5366446157626044 1103 16-17 0.670260542714729 1103 18-19 0.632459942076288 1103 20-21 0.46122892972577034 1103 22-23 0.5891116154775915 1103 24-25 0.5420723241343381 1103 26-27 0.5371313827359003 1103 28-29 0.5664984828299495 1103 30-31 0.7957771934023015 1103 32-33 0.9251809254966403 1103 34-35 0.5769706810966468 1103 36-37 0.5735159170433448 1103 38-39 0.7265485365491244 1103 40-41 0.7525445245113644 1103 42-43 0.6996893568721063 1103 44-45 0.8526582388079973 1103 46-47 0.9273225463646568 1103 48-49 1.1776444396094732 1103 50-51 1.0460827902260732 1103 52-53 0.5822668640465878 1103 54-55 0.7104256170347583 1103 56-57 0.8960382696455156 1103 58-59 0.6531445703900545 1103 60-61 0.7752827941386187 1103 62-63 0.6545947163699246 1103 64-65 0.43341293062493236 1103 66-67 0.05150900840687811 1103 68-69 0.32389807743836485 1103 70-71 0.1006116492373188 1103 72-73 0.5201239871908392 1103 74-75 0.21947980998906758 1103 76-77 0.43497658863191546 1103 78-79 0.5650078132675915 1103 80-81 0.23360349379676038 1103 82-83 0.24918947975271877 1103 84-85 0.5378187480675827 1103 86-87 0.500476950193292 1103 88-89 0.27021574725397457 1103 90-91 0.09893604598529038 1103 92-93 0.3903463824113018 1103 94-95 0.5727896294079429 1103 96-97 0.6213900800070782 1103 98-99 0.7952357222741604 1103 100-101 0.9334502376755687 1104 1 0.28293273892430193 1104 2 0.6807379225272392 1104 3 0.5646087237903146 1104 4 0.2544588134224597 1104 5 0.3402180679534723 1104 6 0.3153961843572901 1104 7 0.27534067588992883 1104 8 0.2768087265367356 1104 9 0.5403022438818041 1104 10-11 0.608865419218688 1104 12-13 0.38118842164708866 1104 14-15 0.5872394085203396 1104 16-17 0.6594775859587827 1104 18-19 0.5786947916947227 1104 20-21 0.6871940651439985 1104 22-23 0.6544873823487549 1104 24-25 0.5067635182088566 1104 26-27 0.6316856622510869 1104 28-29 0.44271893023380215 1104 30-31 0.515434345878738 1104 32-33 0.8105757706260803 1104 34-35 0.716349515452471 1104 36-37 0.5936743414036627 1104 38-39 0.4179015965521202 1104 40-41 0.600778850412901 1104 42-43 0.7484371123711355 1104 44-45 0.6637507496564226 1104 46-47 0.8807397990954655 1104 48-49 0.6640324764192869 1104 50-51 0.4931733558403195 1104 52-53 0.8398420548291696 1104 54-55 0.8187414931383046 1104 56-57 0.5903138569565982 1104 58-59 0.6187569450720929 1104 60-61 0.4688429467646884 1104 62-63 0.1374710443663325 1104 64-65 0.30454584249086025 1104 66-67 0.3787666023018339 1104 68-69 0.1112736946761288 1104 70-71 0.31646250214768656 1104 72-73 0.41430044237563735 1104 74-75 0.5705571704559205 1104 76-77 0.5791062833797831 1104 78-79 0.6411151004140834 1104 80-81 0.5750061198628984 1104 82-83 0.5871692418356176 1104 84-85 0.7679268581977645 1104 86-87 0.824073201876665 1104 88-89 0.9897045621574598 1104 90-91 1.101217955307451 1104 92-93 1.1427779251316323 1104 94-95 1.1955217482168585 1104 96-97 1.12777287964796 1104 98-99 0.9685392536223567 1104 100-101 1.1691742762809874 1105 1 0.29292926505148387 1105 2 0.46649595814357525 1105 3 0.4694360357440921 1105 4 0.22629530371390416 1105 5 0.5070005303807932 1105 6 0.43799646958053273 1105 7 0.3306689568713921 1105 8 0.43473966960178956 1105 9 0.6119988650834003 1105 10-11 0.5478909521839128 1105 12-13 0.6121815378938464 1105 14-15 0.9308922772079526 1105 16-17 0.6987072089521646 1105 18-19 0.6678613364711126 1105 20-21 0.5967225326175765 1105 22-23 0.8746429295751383 1105 24-25 0.7392467430598444 1105 26-27 0.7432461167799218 1105 28-29 0.8998977341245435 1105 30-31 1.046124412430828 1105 32-33 1.0139310289725145 1105 34-35 0.9975058123040483 1105 36-37 0.7949772722553021 1105 38-39 1.0068761641463695 1105 40-41 0.9737310938034014 1105 42-43 1.018908964859527 1105 44-45 1.0238165246957251 1105 46-47 1.0381268068111211 1105 48-49 1.2344503924612624 1105 50-51 1.313275880797356 1105 52-53 1.1019594717304741 1105 54-55 1.2326625813746759 1105 56-57 1.418778816549974 1105 58-59 1.4238064020509142 1105 60-61 1.3671613180668416 1105 62-63 1.3710501389653764 1105 64-65 1.3876662487511453 1105 66-67 1.418905831467736 1105 68-69 1.5085967557065914 1105 70-71 1.2271005520251883 1105 72-73 1.172746295668503 1105 74-75 1.3467666267034062 1105 76-77 1.2128012499209042 1105 78-79 1.3659432351272152 1105 80-81 1.3117151281636268 1105 82-83 1.2693247342113771 1105 84-85 1.5459760957740976 1105 86-87 1.5663266940330267 1105 88-89 1.2255268187121118 1105 90-91 0.8736564311914599 1105 92-93 1.1858585189810498 1105 94-95 0.865212436409351 1105 96-97 0.5424195959239668 1105 98-99 0.6459878768717111 1105 100-101 0.8466903200883529 1106 1 0.07329256099238535 1106 2 0.2602088389520141 1106 3 0.15436868533892678 1106 4 0.13039889334103805 1106 5 -0.039885223033074624 1106 6 0.13063160487223513 1106 7 -0.060494039682879475 1106 8 0.07334386055114095 1106 9 0.0477639809798589 1106 10-11 3.9692485445641523E-4 1106 12-13 -0.004669619216940646 1106 14-15 0.06448429109281761 1106 16-17 0.20324667028796028 1106 18-19 0.1555857895459667 1106 20-21 0.10838285392523517 1106 22-23 0.12391358018492582 1106 24-25 0.004712541845144358 1106 26-27 -0.10376430382121526 1106 28-29 -0.18717349069581246 1106 30-31 -0.23898921313660537 1106 32-33 -0.027521969412383385 1106 34-35 0.062031509270092045 1106 36-37 -0.1689955326868926 1106 38-39 -0.28915395062622906 1106 40-41 -0.34664735684761894 1106 42-43 -0.20289667019002877 1106 44-45 -0.296833042329105 1106 46-47 -0.24238710933745722 1106 48-49 -0.13528166577545875 1106 50-51 -0.07252503636384944 1106 52-53 -0.1962673808401476 1106 54-55 -0.05842139750141939 1106 56-57 0.14170135837025555 1106 58-59 -0.11297157681127601 1106 60-61 0.006203466235412236 1106 62-63 -0.4280306143210417 1106 64-65 -0.4395839592206059 1106 66-67 -0.6445516206408293 1106 68-69 -0.4116475270979123 1106 70-71 -0.41116995723332295 1106 72-73 -0.366428861729613 1106 74-75 -0.44069551548670205 1106 76-77 -0.04405950053849139 1106 78-79 -0.15741564184851597 1106 80-81 -0.2527955067816521 1106 82-83 -0.06478796214523541 1106 84-85 -0.4595748381870486 1106 86-87 -0.8796991338567395 1106 88-89 -0.3329230907999019 1106 90-91 -0.5119544727846801 1106 92-93 -0.3312438262914448 1106 94-95 -0.37004705903612845 1106 96-97 -0.3270007943445776 1106 98-99 -0.2997934828348363 1106 100-101 0.054711888902087225 1107 1 0.14000572682059698 1107 2 0.27609116054237504 1107 3 0.2085664705267014 1107 4 -0.022580258504987682 1107 5 -0.058216860923558045 1107 6 0.18012540511276853 1107 7 0.40563147561201873 1107 8 0.18436485700808447 1107 9 -0.04467279908451616 1107 10-11 0.1903197377911141 1107 12-13 0.19913805963297193 1107 14-15 0.2269942262334368 1107 16-17 0.20095608451438096 1107 18-19 -0.010549458131585254 1107 20-21 0.2166625626025791 1107 22-23 0.16324862672656337 1107 24-25 0.020356188337203207 1107 26-27 0.20576485740961203 1107 28-29 0.21923806395962941 1107 30-31 0.4329310091324743 1107 32-33 0.32952323287056373 1107 34-35 0.06872020510764543 1107 36-37 -0.05209918951380388 1107 38-39 0.0855868088240328 1107 40-41 0.09891850010025394 1107 42-43 0.3682343021908636 1107 44-45 0.08528579006304682 1107 46-47 -0.07356734611241222 1107 48-49 -0.06689893287606452 1107 50-51 -0.05104196028211305 1107 52-53 -0.07195357174778394 1107 54-55 0.1449564344481331 1107 56-57 0.05221209990830289 1107 58-59 0.03400130459964146 1107 60-61 -0.08111454400212637 1107 62-63 -0.1259513602849971 1107 64-65 -0.5283757302593521 1107 66-67 -0.36220361380962274 1107 68-69 -0.4126925996157489 1107 70-71 -0.20318430554602784 1107 72-73 -0.3130108257707782 1107 74-75 0.07840080961195284 1107 76-77 0.1685733638639313 1107 78-79 0.1845339397748944 1107 80-81 -0.07659071891283276 1107 82-83 -0.006537334754138868 1107 84-85 -0.01849167034284349 1107 86-87 -0.08809609457267342 1107 88-89 0.30948483970161433 1107 90-91 0.27245703089160855 1107 92-93 0.5711658653078864 1107 94-95 -0.04033478997745377 1107 96-97 -0.016800793881127873 1107 98-99 0.06577798181923455 1107 100-101 0.678024652921934 1108 1 0.20633990666944868 1108 2 0.34294548848044926 1108 3 0.28970738694895815 1108 4 0.04341298394084703 1108 5 0.26114532387325795 1108 6 0.10763468254402397 1108 7 0.2830426242651001 1108 8 0.5121914684836923 1108 9 0.4823443046085387 1108 10-11 0.20400203658340388 1108 12-13 0.13313279695818636 1108 14-15 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-0.4405097437635135 2316 68-69 -0.9889535257564503 2316 70-71 -0.7437163979903723 2316 72-73 -0.7863524568420353 2316 74-75 -0.2745877904276526 2316 76-77 -1.0551119632838173 2316 78-79 -0.6901895475380542 2316 80-81 -0.7225922464509544 2316 82-83 -1.1617521801616242 2316 84-85 -0.6645455302053627 2316 86-87 -0.21322833977082567 2316 88-89 -0.3260714387629271 2316 90-91 -0.43767988178412764 2316 92-93 -0.6604089133405253 2316 94-95 0.07868730274976343 2316 96-97 0.27524469280948693 2316 98-99 0.31182618413317087 2316 100-101 0.3478529463224014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 18.0 5 37.0 6 74.0 7 211.0 8 445.0 9 819.0 10 1274.0 11 1877.0 12 2504.0 13 3086.0 14 3685.0 15 4446.0 16 5198.0 17 5779.0 18 5579.0 19 5420.0 20 5550.0 21 5699.0 22 6357.0 23 6920.0 24 8201.0 25 9655.0 26 11603.0 27 14037.0 28 17791.0 29 21528.0 30 26274.0 31 30361.0 32 36171.0 33 43401.0 34 52466.0 35 65907.0 36 83542.0 37 88405.0 38 38321.0 39 2440.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39393150996781 16.592409509252334 12.507561221503138 27.506097759276727 2 17.957676637282667 19.06193401119215 35.110572061247915 27.869817290277265 3 20.753950059669556 22.306659252683506 27.29565208908486 29.643738598562074 4 15.38903136937936 15.905864215098738 31.25001016427088 37.45509425125102 5 16.113728665307487 35.2220368798754 31.720854915961876 16.943379538855236 6 35.98949738654007 31.83137289968053 15.514766251818854 16.66436346196054 7 30.887062947295174 29.355082053566495 18.48879668859314 21.26905831054519 8 28.629167303433988 31.7770197988574 18.515947363458118 21.077865534250495 9 27.573587390360682 13.78654982644675 17.092596958143996 41.54726582504857 10-11 27.908048630598 23.184725387994522 24.935374890665695 23.971851090741783 12-13 28.51902010450571 20.45218067067044 24.72743648855607 26.301362736267777 14-15 25.517936545355518 21.499386263687132 22.42868871781949 30.55398847313786 16-17 25.860123624989022 24.28904250787695 23.11091457129572 26.739919295838305 18-19 25.977343005693516 23.31023629216077 24.573636857284995 26.13878384486072 20-21 26.679521237680586 22.457266138393656 24.63224654763724 26.23096607628852 22-23 26.440774135649324 22.818516435100133 24.45251558318674 26.288193846063802 24-25 26.35463315143391 22.942819958590988 24.568859349990692 26.133687539984408 26-27 26.29710529588836 23.132757736295698 24.56039663681377 26.009740331002167 28-29 26.241880193891742 23.23807015447446 24.505780090735353 26.014269560898445 30-31 26.202788601382515 23.256148588830474 24.617931043453307 25.923131766333707 32-33 26.25455124963208 23.268492424476495 24.50820485217281 25.968751473718616 34-35 26.29145043914884 22.981431601643262 24.90152382981041 25.825594129397484 36-37 25.92999643950491 23.2524716176516 24.608061025910324 26.209470916933164 38-39 25.809994285310157 22.947121641603594 24.86906206738457 26.373822005701687 40-41 25.99047105752402 22.766222248235373 25.226822485442796 26.016484208797806 42-43 26.000372337441778 22.735346936386392 24.904659664880043 26.359621061291783 44-45 25.570983765151578 22.97072524327884 25.116294194639327 26.341996796930257 46-47 26.043007203637508 22.78669639931939 24.719264629009825 26.45103176803328 48-49 25.76998088715781 22.889559315254584 24.638331532612558 26.702128264975045 50-51 25.605702027992187 23.1396498451846 24.82071219971431 26.433935927108905 52-53 25.750588824692898 23.320629684572143 24.891587939829275 26.037193550905684 54-55 25.373913382830715 23.158401384105552 24.696088666009736 26.771596567053997 56-57 25.615300191049144 23.621234909150036 24.224055932685662 26.539408967115158 58-59 25.275218467362425 24.01604316336188 24.550056425883707 26.158681943391993 60-61 25.585238853580893 24.259331857392205 24.022568560501927 26.132860728524975 62-63 25.148298292352283 24.513280529105526 23.831230440492718 26.507190738049474 64-65 25.061417063975465 24.247025575050067 23.96408800440722 26.72746935656725 66-67 25.214327972455347 24.279463331708495 23.79339556541489 26.712813130421264 68-69 25.297410520508084 24.07700814659476 23.902130190900976 26.72345114199618 70-71 25.330911907621307 23.760239799256 24.006369260911875 26.90247903221082 72-73 25.415552912734295 23.804263474456736 23.836476247960263 26.943707364848706 74-75 25.334735857496593 23.942912595096107 23.827131020580765 26.895220526826535 76-77 25.418396910099077 23.71472871250324 23.770976908892596 27.095897468505086 78-79 25.449929354303208 23.799714165397106 23.67346241957853 27.07689406072116 80-81 25.431732457566497 23.96858945177863 23.675944592573323 26.923733498081546 82-83 25.522528556982277 23.864955469641643 23.730340147556603 26.882175825819477 84-85 25.495459171563002 23.75927919022706 23.552966577031505 27.192295061178434 86-87 25.22858592131832 23.983231613140276 23.63628825887762 27.151894206663783 88-89 25.276343919946026 23.634294447108935 23.76712161733744 27.322240015607598 90-91 25.240431125729827 23.692729427794244 23.577208673893285 27.48963077258264 92-93 25.369387036635032 23.526862626967265 23.775817253319868 27.32793308307783 94-95 25.37479169207007 23.36552452952892 23.88115270495468 27.37853107344633 96-97 25.23525165586601 23.367545351381757 23.83300871748016 27.564194275272076 98-99 25.026093912070834 23.35397001394927 23.89535772233476 27.724578351645135 100-101 25.12071482686974 23.345515911596102 23.87479149257177 27.65897776896239 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 38.5 2 37.0 3 43.5 4 37.5 5 42.0 6 62.0 7 132.5 8 213.5 9 264.0 10 249.0 11 216.5 12 220.5 13 239.5 14 288.0 15 326.0 16 352.0 17 390.0 18 431.0 19 483.5 20 573.0 21 643.5 22 725.5 23 812.5 24 942.0 25 1123.0 26 1303.0 27 1522.0 28 1759.0 29 1990.5 30 2271.5 31 2588.5 32 2974.5 33 3215.0 34 3296.0 35 3580.5 36 3988.0 37 4408.5 38 4805.0 39 5271.5 40 5804.0 41 6346.5 42 7044.5 43 7884.5 44 8878.0 45 10161.5 46 11867.5 47 13952.0 48 16555.0 49 19730.5 50 23042.5 51 26812.5 52 30794.0 53 34298.5 54 36842.5 55 37281.0 56 36772.5 57 35045.0 58 32634.5 59 30206.5 60 26811.5 61 23229.5 62 19454.5 63 15326.5 64 11584.5 65 8720.0 66 6590.5 67 5305.5 68 4179.5 69 3084.5 70 2213.0 71 1528.5 72 1056.0 73 769.5 74 546.0 75 347.5 76 227.0 77 130.0 78 63.5 79 35.0 80 22.0 81 10.0 82 4.0 83 4.5 84 4.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01723336248914786 2 0.0019509466968846633 3 0.0052025245250257686 4 0.030402252693119337 5 0.0013006311312564421 6 1.6257889140705527E-4 7 0.0 8 0.0 9 1.6257889140705527E-4 10-11 0.0 12-13 0.0 14-15 1.6257889140705527E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.064472285176382E-4 26-27 0.0023573939254023014 28-29 8.128944570352763E-5 30-31 0.00755991845042807 32-33 0.023573939254023013 34-35 0.014550810780931445 36-37 4.8773667422116583E-4 38-39 7.316050113317488E-4 40-41 0.002032236142588191 42-43 0.008535391798870402 44-45 0.00829152346175982 46-47 0.008047655124649236 48-49 0.008860549581684512 50-51 0.1835515683985654 52-53 0.04909882520493069 54-55 0.016908204706333748 56-57 0.009917312375830371 58-59 0.01950946696884663 60-61 0.05478908640417763 62-63 0.099986018215339 64-65 0.10396920105481185 66-67 0.10819625223139528 68-69 0.10689562110013885 70-71 0.09299512588483562 72-73 0.06877087106518438 74-75 0.23256910415779256 76-77 0.2817492188084268 78-79 0.0073160501133174875 80-81 9.754733484423317E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.6257889140705527E-4 90-91 0.0073160501133174875 92-93 0.018615283066107827 94-95 0.001788367805477608 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 615086.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.26822554110235 #Duplication Level Percentage of deduplicated Percentage of total 1 85.25060466971462 60.75659321116576 2 8.556112435820644 12.195579016621924 3 2.5900479765366926 5.537643700622783 4 1.1852562456700786 3.378844377616615 5 0.6479215076801336 2.3088108071139417 6 0.4469121056398657 1.9110379645074542 7 0.2881060598733449 1.4372965358368317 8 0.2165003683031152 1.2343677662366512 9 0.14235764880682913 0.9131019320399524 >10 0.6560876454481567 7.534624438981339 >50 0.014156687549082031 0.6943654856464166 >100 0.004109987739793031 0.5278916742895542 >500 9.133306088085127E-4 0.4341599836537899 >1k 9.133306088085127E-4 1.1356831056670913 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2729 0.44367779464985385 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1899 0.308737314781998 TruSeq Adapter, Index 3 (95% over 21bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 1261 0.20501198206429672 RNA PCR Primer, Index 3 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.17721099163369025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 975 0.15851441912187889 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6257889140705527E-4 2 0.0 0.0 0.0 0.0 1.6257889140705527E-4 3 0.0 0.0 0.0 0.0 1.6257889140705527E-4 4 0.0 0.0 0.0 0.0 1.6257889140705527E-4 5 0.0 0.0 0.0 0.0 1.6257889140705527E-4 6 0.0 0.0 0.0 0.0 3.2515778281411053E-4 7 0.0 0.0 0.0 0.0 4.8773667422116583E-4 8 0.0 0.0 0.0 0.0 4.8773667422116583E-4 9 0.0 0.0 0.0 0.0 4.8773667422116583E-4 10-11 0.0 0.0 0.0 0.0 6.503155656282211E-4 12-13 0.0 0.0 0.0 0.0 0.0018696572511811356 14-15 0.0 0.0 0.0 0.0 0.002357393925402302 16-17 0.0 0.0 0.0 0.0 0.0026012622625128843 18-19 0.0 0.0 0.0 8.128944570352763E-5 0.0026012622625128843 20-21 0.0 0.0 0.0 1.6257889140705527E-4 0.002926420045326995 22-23 0.0 0.0 0.0 4.877366742211658E-4 0.00308899893673405 24-25 0.0 0.0 0.0 0.0017070783597740802 0.003414156719548161 26-27 0.0 0.0 0.0 0.004796077296508131 0.003576735610955216 28-29 0.0 0.0 0.0 0.01203083796412209 0.0037393145023622712 30-31 0.0 0.0 0.0 0.04227051176583437 0.0037393145023622712 32-33 0.0 0.0 0.0 0.1194141957384821 0.0037393145023622712 34-35 0.0 0.0 0.0 0.23208136748357142 0.0039018933937693266 36-37 0.0 0.0 0.0 0.39270931219374206 0.0039018933937693266 38-39 0.0 0.0 0.0 0.61503594619289 0.004389630067990492 40-41 0.0 0.0 0.0 0.8509379176245273 0.00447091951369402 42-43 0.0 0.0 0.0 1.0860269945991292 0.004714787850804603 44-45 0.0 0.0 0.0 1.3652562405907467 0.004877366742211658 46-47 0.0 0.0 0.0 1.67342452925282 0.005039945633618714 48-49 0.0 0.0 0.0 1.9648471920999666 0.0054463928621363514 50-51 0.0 0.0 0.0 2.252124093216233 0.006015418982061045 52-53 0.0 0.0 0.0 2.540701625463756 0.006665734547689266 54-55 0.0 0.0 0.0 2.8655342504950525 0.006665734547689266 56-57 0.0 0.0 0.0 3.2026415818275815 0.006665734547689266 58-59 0.0 0.0 0.0 3.55470617116956 0.006665734547689266 60-61 0.0 0.0 0.0 3.9096158911111614 0.006665734547689266 62-63 0.0 0.0 0.0 4.259648244310552 0.0073160501133174875 64-65 0.0 0.0 0.0 4.633823562883889 0.007803786787538653 66-67 0.0 0.0 0.0 5.037669529139015 0.007966365678945708 68-69 0.0 0.0 0.0 5.468422301922008 0.008128944570352764 70-71 0.0 0.0 0.0 5.920147751696511 0.008128944570352764 72-73 0.0 0.0 0.0 6.387887222274609 0.008372812907463346 74-75 0.0 0.0 0.0 6.870096214187935 0.008454102353166874 76-77 0.0 0.0 0.0 7.37327788309277 0.008535391798870402 78-79 0.0 0.0 0.0 7.922062931037286 0.008779260135980984 80-81 0.0 0.0 0.0 8.494909654910046 0.008779260135980984 82-83 0.0 0.0 0.0 9.088810345220018 0.009104417918795096 84-85 0.0 0.0 0.0 9.687832270609313 0.009185707364498624 86-87 8.128944570352763E-5 0.0 0.0 10.319207395388613 0.009510865147312733 88-89 1.6257889140705527E-4 0.0 0.0 10.984480219026281 0.009836022930126843 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 790 0.0 64.90097 1 GTATCAA 1395 0.0 41.518356 1 CCTAGTA 155 3.6379788E-11 33.691124 2 CTAATAC 115 1.04007995E-7 33.02529 3 TCTATAC 120 1.5111618E-7 31.649239 3 TATCAAC 1825 0.0 30.955557 2 ATCAACG 1895 0.0 29.812078 3 CAACGCA 1905 0.0 29.648352 5 TCAACGC 1925 0.0 29.594091 4 ATTATAC 65 0.005857723 29.214682 3 TAGGACG 65 0.0058647096 29.207556 5 CTAGTAC 150 3.3491233E-8 28.484316 3 AACGCAG 2000 0.0 28.002747 6 TAGTACT 170 3.6561687E-9 27.925798 4 GTACATG 2375 0.0 27.58481 1 TACATGG 2395 0.0 27.354456 2 TAATACT 230 5.456968E-12 26.83305 4 ACATGGG 2555 0.0 24.898228 3 GTGGTAT 325 0.0 24.83248 1 TATACAG 175 1.4952457E-7 24.409172 5 >>END_MODULE