FastQCFastQC Report
Sat 18 Jun 2016
SRR1930959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716167
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67900.18271514708569342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.054.1465071
GTATCAA57200.045.5754851
ATCAACG71250.036.6358683
TCAACGC72750.036.2156874
CAACGCA74700.035.017045
TATCAAC73600.034.790252
AACGCAG80550.032.591516
ACGCAGA90850.028.3082967
GTACATG75100.027.5283831
CGCAGAG94750.027.3099238
TACATGG78250.026.2813382
ACATGGG80450.024.1781393
GCAGAGT103700.023.162819
GTGGTAT13700.022.0834771
CATGGGG61450.020.7408564
CAGAGTA96450.020.1878210-11
AGAGTAC90500.019.80560110-11
GAGTACT65750.019.7058712-13
GAGTACA53350.018.9975851
TGGTATC16900.018.7899462
Produced by FastQC (version 0.11.5)