##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930959_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3716167 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.63789356075763 30.0 10.0 31.0 2.0 33.0 2 21.520087767853276 28.0 10.0 31.0 2.0 33.0 3 21.973854242826008 28.0 10.0 31.0 2.0 33.0 4 23.896170974017046 32.0 2.0 35.0 2.0 37.0 5 23.877798279786674 32.0 10.0 35.0 2.0 37.0 6 24.60625827633688 32.0 11.0 35.0 2.0 37.0 7 24.670288229780848 32.0 11.0 35.0 2.0 37.0 8 25.42867341537665 32.0 17.0 35.0 2.0 37.0 9 26.55056594604064 33.0 17.0 37.0 2.0 39.0 10-11 26.779477483116338 33.0 17.0 37.0 2.0 39.0 12-13 26.704983521999953 33.0 17.0 37.0 2.0 39.0 14-15 26.92027928238962 33.0 16.5 38.0 2.0 40.0 16-17 26.049640933790112 32.5 10.5 38.0 2.0 40.0 18-19 25.430846487792394 32.0 10.0 38.0 2.0 40.0 20-21 24.901309871165637 32.0 9.5 38.0 2.0 40.0 22-23 24.817319835195782 32.0 10.0 38.0 2.0 40.0 24-25 24.517116964872677 31.5 8.0 37.5 2.0 40.0 26-27 24.171731114344432 31.0 5.0 37.0 2.0 39.0 28-29 24.0672449596587 31.0 5.0 37.0 2.0 39.0 30-31 23.95408373735626 30.0 2.0 37.0 2.0 39.0 32-33 23.62026383636688 30.0 2.0 37.0 2.0 39.0 34-35 20.76286036122704 25.0 2.0 36.0 2.0 39.0 36-37 19.151557774448783 17.5 2.0 35.0 2.0 38.5 38-39 18.226570818803353 16.5 2.0 34.0 2.0 38.0 40-41 19.477473025297304 22.0 2.0 34.5 2.0 38.0 42-43 19.37495691124753 21.0 2.0 35.0 2.0 38.0 44-45 17.476889763027337 15.0 2.0 33.5 2.0 38.0 46-47 18.222110847009837 18.5 2.0 33.0 2.0 38.0 48-49 17.649865170214362 15.0 2.0 33.0 2.0 38.0 50-51 15.75396355976467 10.0 2.0 30.5 2.0 37.5 52-53 18.487027897293096 19.0 2.0 32.5 2.0 37.5 54-55 20.07142870597581 26.0 2.0 35.0 2.0 38.5 56-57 20.271727024108444 26.0 2.0 36.0 2.0 39.0 58-59 19.89668628993261 25.5 2.0 35.0 2.0 39.0 60-61 19.560423818412897 25.0 2.0 35.0 2.0 38.5 62-63 19.075237469144955 23.0 2.0 34.0 2.0 38.0 64-65 18.5764158338417 22.0 2.0 34.0 2.0 38.0 66-67 18.150872121731883 21.0 2.0 34.0 2.0 37.5 68-69 17.688106320302612 19.0 2.0 33.0 2.0 37.0 70-71 17.107462474103023 16.0 2.0 32.5 2.0 36.0 72-73 16.629177725328276 11.5 2.0 32.0 2.0 36.0 74-75 16.096935901965654 7.5 2.0 31.5 2.0 35.0 76-77 15.537454856038494 4.5 2.0 31.0 2.0 35.0 78-79 15.035427498279812 2.0 2.0 31.0 2.0 34.5 80-81 14.532560969407456 2.0 2.0 30.0 2.0 34.0 82-83 14.054977077187328 2.0 2.0 30.0 2.0 34.0 84-85 13.53856688894767 2.0 2.0 29.5 2.0 34.0 86-87 12.988111002546441 2.0 2.0 29.0 2.0 34.0 88-89 12.442750150894724 2.0 2.0 29.0 2.0 34.0 90-91 11.878796485733822 2.0 2.0 27.5 2.0 34.0 92-93 11.268558705784752 2.0 2.0 26.0 2.0 33.0 94-95 10.628812698675812 2.0 2.0 25.0 2.0 33.0 96-97 9.924557749961183 2.0 2.0 23.5 2.0 32.0 98-99 9.165839694502427 2.0 2.0 15.0 2.0 32.0 100-101 8.179419681623566 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.794432951261182 1101 2 7.809884994290751 1101 3 7.330624612282389 1101 4 9.214594293549585 1101 5 8.899493306187498 1101 6 8.460201441767818 1101 7 8.274038618443882 1101 8 7.3492653581144936 1101 9 7.409539652695795 1101 10-11 6.914021234776641 1101 12-13 6.766978463991318 1101 14-15 7.316160609884893 1101 16-17 7.94693661205352 1101 18-19 8.449459256748959 1101 20-21 8.80099920696976 1101 22-23 8.81125196807994 1101 24-25 8.708269014364404 1101 26-27 8.534279964682685 1101 28-29 8.540773194646672 1101 30-31 8.4137700272947 1101 32-33 8.292873103670996 1101 34-35 10.683622571161894 1101 36-37 5.151061714095086 1101 38-39 8.043007264633403 1101 40-41 9.585494615639679 1101 42-43 9.910096651238842 1101 44-45 4.273764423369695 1101 46-47 5.8434353574888895 1101 48-49 8.938457760063773 1101 50-51 -3.4998849329218604 1101 52-53 5.712181664762017 1101 54-55 8.261568015185485 1101 56-57 8.774873479227299 1101 58-59 8.958096332478458 1101 60-61 8.709425269475023 1101 62-63 8.814036298439415 1101 64-65 8.790281921853445 1101 66-67 8.671208608655398 1101 68-69 8.588473136010013 1101 70-71 8.731115818129716 1101 72-73 8.543523522334773 1101 74-75 8.54950459160104 1101 76-77 8.414585065466943 1101 78-79 8.20925968114335 1101 80-81 7.975804942238861 1101 82-83 7.698282491752165 1101 84-85 7.623104756572369 1101 86-87 7.333271454630754 1101 88-89 7.19865710653478 1101 90-91 7.115585366576786 1101 92-93 7.219429075092187 1101 94-95 6.94950967072381 1101 96-97 6.777536120277151 1101 98-99 6.345217778858817 1101 100-101 5.840334787018465 1102 1 6.547850777710433 1102 2 7.749202454356055 1102 3 7.34710178080023 1102 4 9.144611565729388 1102 5 9.183436316659115 1102 6 8.582350124042026 1102 7 8.262447050618078 1102 8 7.379023619805345 1102 9 7.311074436064242 1102 10-11 6.959704056322963 1102 12-13 6.426721179276182 1102 14-15 6.993993442334645 1102 16-17 7.728360353791103 1102 18-19 8.136520863263868 1102 20-21 8.487627709243593 1102 22-23 8.211367501055218 1102 24-25 8.27267796210451 1102 26-27 8.377720507803208 1102 28-29 8.227389060501334 1102 30-31 8.063223592425697 1102 32-33 8.131282113510071 1102 34-35 10.31634710588894 1102 36-37 1.0287725509437493 1102 38-39 3.318255263385648 1102 40-41 8.314496786215447 1102 42-43 9.325125216766981 1102 44-45 1.1278470698835577 1102 46-47 1.5726745150801982 1102 48-49 7.242023215992997 1102 50-51 -4.617322072152094 1102 52-53 4.54325909640621 1102 54-55 7.268508405946886 1102 56-57 7.658553977116654 1102 58-59 8.05186390612712 1102 60-61 8.031939938552476 1102 62-63 7.9592201258447055 1102 64-65 7.921211687913612 1102 66-67 7.76854994216588 1102 68-69 7.864057046887876 1102 70-71 7.6632310608283944 1102 72-73 7.803438092920366 1102 74-75 7.543908427006988 1102 76-77 7.541065801065399 1102 78-79 7.533779533318908 1102 80-81 7.497908999901371 1102 82-83 7.290693852687289 1102 84-85 7.227104219344033 1102 86-87 7.053833724738183 1102 88-89 6.7948201481136525 1102 90-91 6.591134545353958 1102 92-93 6.468125574298618 1102 94-95 6.121112929042546 1102 96-97 5.918691240626405 1102 98-99 5.718207649505208 1102 100-101 5.352894737801419 1103 1 6.4297085567726135 1103 2 7.493775367070583 1103 3 7.146711418652654 1103 4 8.846250205131827 1103 5 8.767903500071863 1103 6 8.084662750070361 1103 7 7.756762449375309 1103 8 6.997184571591582 1103 9 7.132414322659518 1103 10-11 6.628896500439545 1103 12-13 6.456224272401176 1103 14-15 6.977088922059838 1103 16-17 7.5320213474048785 1103 18-19 7.918508434430521 1103 20-21 8.226727411718851 1103 22-23 7.999749474036047 1103 24-25 8.06032608720291 1103 26-27 7.989645917903161 1103 28-29 7.844584669427132 1103 30-31 7.68051429913638 1103 32-33 7.601826305214811 1103 34-35 9.880169408724228 1103 36-37 -3.1168062435388464 1103 38-39 -2.019250049556131 1103 40-41 6.033552861305591 1103 42-43 8.482025541613407 1103 44-45 0.5441801021149821 1103 46-47 0.19060569709813535 1103 48-49 6.7615193181456945 1103 50-51 -5.149782667657872 1103 52-53 3.8382245401033934 1103 54-55 6.919319515058167 1103 56-57 7.411885005006464 1103 58-59 7.6100079804331 1103 60-61 7.461975044105738 1103 62-63 7.4104350326143695 1103 64-65 7.406450511864197 1103 66-67 7.305358207004868 1103 68-69 7.277262027434556 1103 70-71 7.35646071116993 1103 72-73 7.061383176607826 1103 74-75 7.07228604964264 1103 76-77 6.953833141676439 1103 78-79 6.815957511305514 1103 80-81 6.562217072502703 1103 82-83 6.398435523586 1103 84-85 6.243780589435181 1103 86-87 6.129407909742071 1103 88-89 6.088065839384766 1103 90-91 6.031539604880029 1103 92-93 5.763535206686708 1103 94-95 5.5601122947287 1103 96-97 5.452472220111762 1103 98-99 5.05557251629226 1103 100-101 4.6759289915939934 1104 1 6.359575875425335 1104 2 7.337627984625932 1104 3 6.934703758431077 1104 4 8.754591877781973 1104 5 8.545099147443555 1104 6 7.978662982922518 1104 7 7.8063743681283455 1104 8 6.794098153735305 1104 9 7.005541032134108 1104 10-11 6.321630727693012 1104 12-13 6.120221982119208 1104 14-15 6.5956815526644625 1104 16-17 7.095648815356114 1104 18-19 7.673773607633954 1104 20-21 7.896689687619979 1104 22-23 7.922858856168634 1104 24-25 7.942703856035337 1104 26-27 7.875338210940438 1104 28-29 7.803240743710386 1104 30-31 7.389636333813598 1104 32-33 7.314700264251268 1104 34-35 9.466391320932136 1104 36-37 -3.3518002703004566 1104 38-39 -2.2743960652746757 1104 40-41 5.536923857281206 1104 42-43 7.802672017061337 1104 44-45 0.15450497868114255 1104 46-47 -0.5182892555068896 1104 48-49 6.045247117382061 1104 50-51 -5.230843695068531 1104 52-53 3.492228999802723 1104 54-55 6.164151135972116 1104 56-57 6.885837212953689 1104 58-59 7.170710490627208 1104 60-61 6.9201843631770465 1104 62-63 6.833362264265055 1104 64-65 6.922402353120479 1104 66-67 6.83576719714689 1104 68-69 6.761618903077501 1104 70-71 6.704093181686986 1104 72-73 6.581069802665347 1104 74-75 6.503783913855683 1104 76-77 6.3978551745441035 1104 78-79 6.194854003739353 1104 80-81 5.966145547966333 1104 82-83 5.882372036204918 1104 84-85 5.701428844051167 1104 86-87 5.475822925360578 1104 88-89 5.493647189633682 1104 90-91 5.249376353662933 1104 92-93 4.940210453375137 1104 94-95 4.74349405926327 1104 96-97 4.516479694597891 1104 98-99 4.211122370042666 1104 100-101 3.7882671175164813 1105 1 5.880398522041357 1105 2 7.082932405451093 1105 3 6.705212391003528 1105 4 8.585092684830503 1105 5 8.472495565180882 1105 6 7.5856829148364895 1105 7 7.3800966957648875 1105 8 6.620647402233423 1105 9 6.697424467578831 1105 10-11 5.997926010795567 1105 12-13 5.7978389313301015 1105 14-15 6.0096230677971185 1105 16-17 6.661836030753996 1105 18-19 7.20464344573432 1105 20-21 7.61942928967024 1105 22-23 7.409798407637677 1105 24-25 7.3299700668531 1105 26-27 7.406686485154211 1105 28-29 7.245442496574778 1105 30-31 7.097355067800073 1105 32-33 6.866114419842813 1105 34-35 9.057201593686731 1105 36-37 -3.635285468672855 1105 38-39 -2.477509028235186 1105 40-41 5.032714294782284 1105 42-43 7.094308835952756 1105 44-45 -3.320977495985211 1105 46-47 -4.390486731477498 1105 48-49 4.181945183246228 1105 50-51 -9.454406797789458 1105 52-53 0.14231076394858277 1105 54-55 4.70409848251041 1105 56-57 5.66555900961983 1105 58-59 6.020179454016734 1105 60-61 6.28134692354017 1105 62-63 6.27536976935297 1105 64-65 6.126058435247433 1105 66-67 5.918318662214734 1105 68-69 5.884396110014279 1105 70-71 5.860369159182319 1105 72-73 5.658710751473709 1105 74-75 5.617601226933578 1105 76-77 5.666350224337476 1105 78-79 5.466067132081919 1105 80-81 5.218136587675241 1105 82-83 5.101643315737666 1105 84-85 4.792410424277351 1105 86-87 4.662975659460926 1105 88-89 4.5183002382115145 1105 90-91 4.429831048381432 1105 92-93 4.204611304251898 1105 94-95 3.8454150201475663 1105 96-97 3.6102113307679637 1105 98-99 3.3166709445658835 1105 100-101 3.166421624607784 1106 1 5.735703288537504 1106 2 6.872650863870632 1106 3 6.496598399410519 1106 4 7.857279481666378 1106 5 7.72384579649292 1106 6 7.0231380763280065 1106 7 6.877195533634048 1106 8 6.159988930840001 1106 9 6.149854779816248 1106 10-11 5.542823870104954 1106 12-13 5.474216703508308 1106 14-15 5.7669760625669575 1106 16-17 6.289550940618838 1106 18-19 6.601095255465985 1106 20-21 7.180879912385365 1106 22-23 7.019852541839267 1106 24-25 7.007439451718227 1106 26-27 6.873665038680848 1106 28-29 6.80992612876209 1106 30-31 6.466331089024759 1106 32-33 6.310016434751322 1106 34-35 8.78149162243544 1106 36-37 -0.12093740626910332 1106 38-39 0.6498552431917624 1106 40-41 6.21826411251277 1106 42-43 7.326661336132371 1106 44-45 -3.4999014967307414 1106 46-47 -4.515229674716242 1106 48-49 3.8462506588285663 1106 50-51 -9.428283713545644 1106 52-53 -0.21856491064644956 1106 54-55 4.231562557038391 1106 56-57 5.221125700686283 1106 58-59 5.75964715519261 1106 60-61 5.709015185737545 1106 62-63 5.714208612074319 1106 64-65 5.418279910667341 1106 66-67 5.370207007690691 1106 68-69 5.150242910952645 1106 70-71 5.077384937131093 1106 72-73 5.1130684037215985 1106 74-75 5.037318951277099 1106 76-77 4.887035601439093 1106 78-79 4.935798080206171 1106 80-81 4.766136829243516 1106 82-83 4.591086547712974 1106 84-85 4.219448864870818 1106 86-87 3.9596782524673166 1106 88-89 3.9324827170144214 1106 90-91 3.9962832798163372 1106 92-93 3.8016553727614966 1106 94-95 3.792141275391524 1106 96-97 3.420752428091868 1106 98-99 3.1669967493683036 1106 100-101 2.9218488412310126 1107 1 5.527799099322877 1107 2 6.809764042713798 1107 3 6.31174918830196 1107 4 7.541229431624565 1107 5 7.792166500225843 1107 6 6.43430460087524 1107 7 6.5211950933895295 1107 8 5.924333393376532 1107 9 6.071517484625094 1107 10-11 5.603734091080966 1107 12-13 5.2656182034255 1107 14-15 5.605129627673261 1107 16-17 6.213618146825048 1107 18-19 6.551634606434472 1107 20-21 7.055032853126189 1107 22-23 6.811963653178957 1107 24-25 6.962836407242214 1107 26-27 6.952014934328496 1107 28-29 6.844936754289893 1107 30-31 6.6767603840060765 1107 32-33 6.241833693159425 1107 34-35 8.47601271251379 1107 36-37 -0.2996350230856244 1107 38-39 0.3507499784638881 1107 40-41 6.078382587662098 1107 42-43 7.071993381304754 1107 44-45 -3.5859062982761234 1107 46-47 -4.662346818845922 1107 48-49 3.777647473433163 1107 50-51 -9.458974053984264 1107 52-53 -0.23001641123224204 1107 54-55 4.247191880409307 1107 56-57 5.265148609859107 1107 58-59 5.678084914594866 1107 60-61 5.74246502614891 1107 62-63 5.677677317169124 1107 64-65 5.822729234717137 1107 66-67 5.766688214495556 1107 68-69 5.578940913497423 1107 70-71 5.658804972576945 1107 72-73 5.4764732836469605 1107 74-75 5.415670029824744 1107 76-77 5.448445726240186 1107 78-79 5.4147436174330625 1107 80-81 5.275763963735132 1107 82-83 4.934929861807893 1107 84-85 4.8016951302498025 1107 86-87 4.576719135548451 1107 88-89 4.441235276964308 1107 90-91 4.360715868402691 1107 92-93 4.069159978356922 1107 94-95 3.8993976468509253 1107 96-97 3.8235822077535992 1107 98-99 3.6397149609880834 1107 100-101 3.1178067690782267 1108 1 6.008461864058265 1108 2 6.2943412935608976 1108 3 6.487654421608273 1108 4 7.940347468456224 1108 5 8.044992296865171 1108 6 7.224979190600774 1108 7 7.2143330180999214 1108 8 6.414960688257203 1108 9 4.640010472377799 1108 10-11 5.658629139497435 1108 12-13 5.237861098136026 1108 14-15 5.920440157081362 1108 16-17 5.859364027965864 1108 18-19 6.677060841951544 1108 20-21 7.480606172029777 1108 22-23 7.444834862077652 1108 24-25 7.132782624383616 1108 26-27 7.4509375314165744 1108 28-29 7.185084686886167 1108 30-31 7.026560915631851 1108 32-33 7.018572705932616 1108 34-35 9.228088448467872 1108 36-37 -3.5135978668333276 1108 38-39 -2.342771337596382 1108 40-41 5.1935803537800105 1108 42-43 7.539260469540974 1108 44-45 -3.237842458164927 1108 46-47 -4.247409916154361 1108 48-49 4.634472913762494 1108 50-51 -9.438588447526996 1108 52-53 0.37607476320183864 1108 54-55 5.136233288849272 1108 56-57 6.395046849206832 1108 58-59 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3.763821551144641 1109 20-21 4.268534873423921 1109 22-23 5.878898459800187 1109 24-25 5.801145451141018 1109 26-27 4.705091751071333 1109 28-29 4.657593273822467 1109 30-31 4.483474255851835 1109 32-33 4.1291600403598885 1109 34-35 6.461958663546394 1109 36-37 -4.61469727395559 1109 38-39 -3.399774241096333 1109 40-41 2.915736711522637 1109 42-43 3.6447802694232188 1109 44-45 -4.572497829509938 1109 46-47 -5.900132000127567 1109 48-49 1.7143517013263825 1109 50-51 -9.691353819336385 1109 52-53 -1.3620589302897557 1109 54-55 2.204891661440964 1109 56-57 3.7866079293828534 1109 58-59 4.345252934832885 1109 60-61 4.643053268314656 1109 62-63 4.712124302038628 1109 64-65 4.238834505662879 1109 66-67 4.458233888280748 1109 68-69 4.483620274789999 1109 70-71 4.74743614136429 1109 72-73 4.551094729305298 1109 74-75 4.72058805622815 1109 76-77 4.699517171963423 1109 78-79 4.756725378291597 1109 80-81 4.5056143332036545 1109 82-83 4.246959102583515 1109 84-85 4.164008659949415 1109 86-87 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-10.203546589099114 2313 90-91 -9.625778676366348 2313 92-93 -9.046052985716816 2313 94-95 -8.528433495717326 2313 96-97 -7.802321479597193 2313 98-99 -7.0238394015088845 2313 100-101 -6.048040340602322 2314 1 -20.49217056700675 2314 2 -19.36819349014768 2314 3 -19.80604656498419 2314 4 -21.69663710022579 2314 5 -21.681291538196806 2314 6 -22.411246460803223 2314 7 -22.47021036667114 2314 8 -23.22556549438889 2314 9 -24.346076044191893 2314 10-11 -24.562974705683445 2314 12-13 -24.493102726807056 2314 14-15 -24.702377955744335 2314 16-17 -23.830871236390323 2314 18-19 -23.218335059895153 2314 20-21 -22.708743688835387 2314 22-23 -22.601332605668365 2314 24-25 -22.30296237941097 2314 26-27 -21.9616604953985 2314 28-29 -21.85665576340475 2314 30-31 -21.743740121555096 2314 32-33 -21.407938804312767 2314 34-35 -18.675782029650012 2314 36-37 -17.03459457819179 2314 38-39 -16.1526625594532 2314 40-41 -17.38054056703962 2314 42-43 -17.296939884620425 2314 44-45 -15.384692951461158 2314 46-47 -16.077538932091624 2314 48-49 -15.566007733846916 2314 50-51 -13.653741495844729 2314 52-53 -16.356153923871272 2314 54-55 -17.879703973989077 2314 56-57 -18.06409810399327 2314 58-59 -17.68452883666904 2314 60-61 -17.360669453123084 2314 62-63 -16.890699831465863 2314 64-65 -16.394156508457787 2314 66-67 -15.963974070640948 2314 68-69 -15.498536336249796 2314 70-71 -14.918545886877048 2314 72-73 -14.444027221914212 2314 74-75 -13.931144934785971 2314 76-77 -13.386362160514112 2314 78-79 -12.88884586689454 2314 80-81 -12.376157168721894 2314 82-83 -11.898740512307372 2314 84-85 -11.3902917251597 2314 86-87 -10.852249519658827 2314 88-89 -10.312303651246006 2314 90-91 -9.745578368199938 2314 92-93 -9.146335471592522 2314 94-95 -8.528433495717326 2314 96-97 -7.825305557666017 2314 98-99 -7.079951368637801 2314 100-101 -6.111086051953118 2315 1 -20.49217056700675 2315 2 -19.36819349014768 2315 3 -19.80604656498419 2315 4 -16.738919321315958 2315 5 -21.681291538196806 2315 6 -22.411246460803223 2315 7 -22.47021036667114 2315 8 -23.22556549438889 2315 9 -19.744700598445586 2315 10-11 -18.221787746946816 2315 12-13 -14.793281025329726 2315 14-15 -14.67066628992671 2315 16-17 -19.872516677042384 2315 18-19 -23.218335059895153 2315 20-21 -22.708743688835387 2315 22-23 -22.601332605668365 2315 24-25 -22.30296237941097 2315 26-27 -21.9616604953985 2315 28-29 -21.85665576340475 2315 30-31 -21.743740121555096 2315 32-33 -21.407938804312767 2315 34-35 -18.675782029650012 2315 36-37 -17.03459457819179 2315 38-39 -16.1526625594532 2315 40-41 -17.38054056703962 2315 42-43 -17.296939884620425 2315 44-45 -15.384692951461158 2315 46-47 -16.077538932091624 2315 48-49 -15.566007733846916 2315 50-51 -13.653741495844729 2315 52-53 -16.356153923871272 2315 54-55 -17.879703973989077 2315 56-57 -18.06409810399327 2315 58-59 -17.68452883666904 2315 60-61 -17.360669453123084 2315 62-63 -16.890699831465863 2315 64-65 -16.394156508457787 2315 66-67 -15.963974070640948 2315 68-69 -15.498536336249796 2315 70-71 -14.918545886877048 2315 72-73 -14.444027221914212 2315 74-75 -13.931144934785971 2315 76-77 -13.386362160514112 2315 78-79 -12.88884586689454 2315 80-81 -12.376157168721894 2315 82-83 -11.898740512307372 2315 84-85 -11.3902917251597 2315 86-87 -10.852249519658827 2315 88-89 -10.312303651246006 2315 90-91 -9.745578368199938 2315 92-93 -9.146335471592522 2315 94-95 -8.528433495717326 2315 96-97 -7.825305557666017 2315 98-99 -7.079951368637801 2315 100-101 -6.111086051953118 2316 1 -20.49217056700675 2316 2 -19.36819349014768 2316 3 -19.80604656498419 2316 4 -21.69663710022579 2316 5 -21.681291538196806 2316 6 -22.411246460803223 2316 7 -22.47021036667114 2316 8 -23.22556549438889 2316 9 -24.346076044191893 2316 10-11 -24.562974705683445 2316 12-13 -24.493102726807056 2316 14-15 -24.702377955744335 2316 16-17 -23.830871236390323 2316 18-19 -23.218335059895153 2316 20-21 -22.708743688835387 2316 22-23 -22.601332605668365 2316 24-25 -22.30296237941097 2316 26-27 -21.9616604953985 2316 28-29 -21.85665576340475 2316 30-31 -21.743740121555096 2316 32-33 -21.407938804312767 2316 34-35 -18.675782029650012 2316 36-37 -17.03459457819179 2316 38-39 -16.1526625594532 2316 40-41 -17.38054056703962 2316 42-43 -17.296939884620425 2316 44-45 -15.384692951461158 2316 46-47 -16.077538932091624 2316 48-49 -15.566007733846916 2316 50-51 -13.653741495844729 2316 52-53 -16.356153923871272 2316 54-55 -17.879703973989077 2316 56-57 -18.06409810399327 2316 58-59 -17.68452883666904 2316 60-61 -17.360669453123084 2316 62-63 -16.890699831465863 2316 64-65 -16.394156508457787 2316 66-67 -15.963974070640948 2316 68-69 -15.498536336249796 2316 70-71 -14.918545886877048 2316 72-73 -14.444027221914212 2316 74-75 -13.931144934785971 2316 76-77 -13.386362160514112 2316 78-79 -12.88884586689454 2316 80-81 -12.376157168721894 2316 82-83 -11.898740512307372 2316 84-85 -11.3902917251597 2316 86-87 -10.852249519658827 2316 88-89 -10.312303651246006 2316 90-91 -9.745578368199938 2316 92-93 -9.146335471592522 2316 94-95 -8.528433495717326 2316 96-97 -7.825305557666017 2316 98-99 -7.079951368637801 2316 100-101 -6.111086051953118 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 633844.0 3 40490.0 4 81415.0 5 51503.0 6 45516.0 7 56475.0 8 72024.0 9 84750.0 10 87519.0 11 80287.0 12 70778.0 13 64639.0 14 62603.0 15 66325.0 16 70924.0 17 75260.0 18 78089.0 19 81096.0 20 84255.0 21 89478.0 22 94168.0 23 98828.0 24 104468.0 25 110198.0 26 115447.0 27 121374.0 28 129078.0 29 134238.0 30 137745.0 31 135817.0 32 131647.0 33 126089.0 34 114037.0 35 93692.0 36 63175.0 37 25590.0 38 3290.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18705657142911 19.260664414801077 13.478055020659601 24.07422399311021 2 15.702065761410152 20.299076506554655 40.27812577441169 23.720731957623507 3 19.112290831920163 25.087052329044457 29.733325955557582 26.067330883477798 4 12.713638622184098 16.585829413169872 38.02203312096241 32.67849884368362 5 13.505046010704941 37.347837042209626 35.18859287137757 13.958524075707862 6 31.444369501296578 36.7802537593562 17.73402783984342 14.041348899503811 7 27.262753946187328 32.22932355739795 22.45874667511344 18.049175821301276 8 25.57312210582106 35.67999705890913 20.533991810900112 18.212889024369694 9 26.244543593791896 15.983974001331244 20.6130716145855 37.158410790291356 10-11 25.08137209775252 26.108460797083623 28.52401672261419 20.286150382549675 12-13 25.387979164107776 23.889855064372412 28.59201402458769 22.13015174693212 14-15 22.509304574840197 25.26958178499487 26.22441514620436 25.99669849396057 16-17 22.408020239649456 28.218897757409454 26.868025058758576 22.50505694418251 18-19 22.42935777269194 27.234179301018486 28.693649854852787 21.642813071436787 20-21 22.94817993344677 26.522625497611273 28.77008123502093 21.75911333392103 22-23 22.69608957550803 26.374020242732655 28.613206757317023 22.316683424442292 24-25 22.546605081361605 26.631265532104305 28.5077286666926 22.314400719841494 26-27 22.45300156106631 26.789768249317248 28.396046950284635 22.36118323933181 28-29 22.455390644058344 27.073590626246357 28.35083425942777 22.120184470267528 30-31 22.66980373061723 26.89294855325691 28.255340148837902 22.181907567287958 32-33 22.485684954173255 27.00487878956956 28.133036434371974 22.376399821885215 34-35 22.91185640346686 26.917626717347375 28.147330187143147 22.02318669204261 36-37 23.325255256878403 26.9406301254066 27.695674788160936 22.03843982955406 38-39 23.057143918015743 26.96445135057125 27.79910059997366 22.17930413143935 40-41 23.0572950963146 26.887912868891185 27.910445033174085 22.144347001620133 42-43 23.158776938753654 26.90909796872144 27.591795650379158 22.34032944214575 44-45 23.348886235637465 27.13970600527385 27.359863305738152 22.15154445335053 46-47 22.88312327280302 27.060351685274505 27.553902762093546 22.502622279828934 48-49 23.26461125226935 27.18917302079187 27.0581876427391 22.488028084199684 50-51 23.49438305242895 27.3281156580879 26.957620127156034 22.21988116232712 52-53 22.65057120282665 27.329296562094434 27.418965476405205 22.601166758673713 54-55 22.374058866755576 27.219379612882793 27.6296060786754 22.776955441686233 56-57 22.485737044931 27.423472133799592 27.385504224295154 22.705286596974254 58-59 22.53707402106191 27.360843845770084 27.46499563194287 22.63708650122514 60-61 22.472773874789496 27.3829659908356 27.561274237062644 22.582985897312255 62-63 22.459309559407163 27.263560725224316 27.55079372582897 22.72633598953955 64-65 22.278863416601155 27.194050740263272 27.56580126652012 22.961284576615455 66-67 22.321780701195966 27.352364356216942 27.414582440111644 22.911272502475455 68-69 22.437159370855536 27.23777251301817 27.455017586542912 22.87005052958338 70-71 22.29951294013463 27.110814293567625 27.389668977137617 23.200003789160128 72-73 22.399575876215316 27.286631682016377 27.289741316346134 23.024051125422172 74-75 22.389847652230102 27.44821205220836 27.167450759734713 22.994489535826823 76-77 22.47579984565002 27.224454067817693 27.202703509358432 23.097042577173852 78-79 22.39721674664837 27.26032502626546 27.18328375302106 23.15917447406511 80-81 22.51303316667806 27.30585738767858 27.06853840557774 23.11257104006562 82-83 22.6024227665716 27.230165824371998 27.116673174318624 23.050738234737782 84-85 22.555111818213426 27.310338254142003 26.968013744534375 23.166536183110193 86-87 22.406865067757213 27.347421134523408 26.981495709107524 23.26421808861186 88-89 22.485150338902297 27.185357078150364 26.82469194997996 23.504800632967378 90-91 22.488903872003483 27.186896020989803 26.888409844321387 23.43579026268533 92-93 22.57914847327156 27.016001087205137 26.97309227956946 23.43175815995384 94-95 22.40893913171804 26.956710399041917 26.976872849484117 23.657477619755923 96-97 22.396382456418067 26.820221243295727 26.927540977861774 23.855855322424432 98-99 22.262925239672693 26.94318747152678 26.79324547734802 24.000641811452503 100-101 22.6431111284924 26.968595753123765 26.626831974799416 23.761461143584427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44334.0 1 48270.0 2 36095.0 3 21184.5 4 27249.0 5 34362.0 6 36439.5 7 35036.5 8 32529.0 9 29749.5 10 26478.0 11 23304.0 12 20205.5 13 17047.5 14 14510.0 15 12527.5 16 10985.5 17 10073.0 18 9880.0 19 10031.0 20 10588.5 21 11726.0 22 13254.5 23 15517.0 24 18810.0 25 22665.5 26 27047.0 27 32339.5 28 38724.5 29 45904.0 30 52972.0 31 60086.0 32 67726.0 33 74024.0 34 78266.5 35 82608.0 36 87442.0 37 91896.5 38 95581.0 39 98358.0 40 100470.0 41 102632.5 42 104835.0 43 107114.5 44 109680.5 45 112511.0 46 114640.5 47 115357.5 48 115595.5 49 115123.0 50 112965.0 51 109967.0 52 106814.5 53 103346.0 54 98061.0 55 90579.0 56 82156.0 57 72862.0 58 64016.5 59 55789.0 60 47380.5 61 39222.0 62 31588.0 63 24783.5 64 19195.5 65 14621.0 66 10997.0 67 8259.5 68 6147.0 69 4470.5 70 3281.5 71 2437.0 72 1741.5 73 1225.0 74 853.0 75 617.5 76 431.0 77 262.5 78 165.5 79 122.5 80 71.0 81 40.5 82 27.0 83 15.0 84 8.0 85 7.5 86 8.5 87 5.0 88 1.5 89 3.0 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.574533383456664 2 21.599271507443017 3 17.32637419147202 4 22.60996343813397 5 22.46618626127405 6 16.298002753912836 7 15.13118221005676 8 12.16500765439228 9 3.0547873655839473 10-11 6.578310393478011 12-13 3.602825169051875 14-15 7.490029377043604 16-17 11.606784625125835 18-19 14.71404541292143 20-21 15.139281953690457 22-23 13.059921688126503 24-25 15.605353580719058 26-27 13.103932089166067 28-29 13.131931369069259 30-31 14.009327352618975 32-33 12.464684714115377 34-35 19.350664273161026 36-37 24.077079959000766 38-39 23.179125157723 40-41 18.28996113468528 42-43 18.220938402391496 44-45 23.540101938368217 46-47 12.928186488927974 48-49 21.68682139419461 50-51 21.326140617469562 52-53 11.38110854544481 54-55 12.992715343524658 56-57 12.505815804295125 58-59 13.53728182829243 60-61 12.944493613984518 62-63 12.905663281547897 64-65 13.980372787337059 66-67 12.288589291062538 68-69 13.330415452265735 70-71 11.939022116067441 72-73 11.30087802835556 74-75 11.382104194994467 76-77 11.417611210691016 78-79 11.840183716178524 80-81 11.817095410405399 82-83 10.30393682522879 84-85 10.221795737382093 86-87 10.35342329879147 88-89 11.776677420578785 90-91 9.242897318661944 92-93 11.292307369394324 94-95 11.380449263986254 96-97 10.924293768283286 98-99 10.778619475389561 100-101 10.893361358625702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3716167.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.77928665473836 #Duplication Level Percentage of deduplicated Percentage of total 1 98.78765896576945 95.60599164998736 2 0.8190745138581973 1.5853889433654587 3 0.1757403389032364 0.5102407390655155 4 0.07786799947478337 0.30144037769604304 5 0.038489291866280036 0.18624831053323024 6 0.02246312408849785 0.1304379075193015 7 0.01491231602222966 0.10102423149009883 8 0.011253481628421517 0.08712831395046704 9 0.010516391781765123 0.09159920053388834 >10 0.0384433065812092 0.6099983323059308 >50 0.0018866074102666737 0.13266931485331657 >100 0.0015546484010882087 0.32517661740067055 >500 5.5605698098471676E-5 0.040495630720057574 >1k 5.560567763059985E-5 0.10945925875797047 >5k 2.7802838815299927E-5 0.18270117182070295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6790 0.18271514708569342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.690944728802554E-5 2 0.0 0.0 0.0 0.0 2.690944728802554E-5 3 0.0 0.0 0.0 0.0 2.690944728802554E-5 4 0.0 0.0 0.0 0.0 2.690944728802554E-5 5 2.690944728802554E-5 0.0 0.0 0.0 2.690944728802554E-5 6 2.690944728802554E-5 0.0 0.0 0.0 1.0763778915210216E-4 7 2.690944728802554E-5 0.0 0.0 0.0 1.0763778915210216E-4 8 2.690944728802554E-5 0.0 0.0 0.0 1.345472364401277E-4 9 2.690944728802554E-5 0.0 0.0 2.690944728802554E-5 1.345472364401277E-4 10-11 2.690944728802554E-5 0.0 0.0 5.381889457605108E-5 2.0182085466019154E-4 12-13 2.690944728802554E-5 0.0 0.0 5.381889457605108E-5 5.516436694045234E-4 14-15 2.690944728802554E-5 0.0 0.0 5.381889457605108E-5 6.592814585566257E-4 16-17 2.690944728802554E-5 0.0 0.0 5.381889457605108E-5 7.803739713527407E-4 18-19 2.690944728802554E-5 0.0 0.0 5.381889457605108E-5 8.072834186407662E-4 20-21 2.690944728802554E-5 0.0 0.0 1.0763778915210216E-4 9.687401023689194E-4 22-23 2.690944728802554E-5 0.0 0.0 1.6145668372815323E-4 0.0010629231678770086 24-25 2.690944728802554E-5 0.0 0.0 2.1527557830420432E-4 0.0011032873388090471 26-27 2.690944728802554E-5 0.0 0.0 3.6327753838834477E-4 0.0011436515097410854 28-29 2.690944728802554E-5 0.0 0.0 0.0010360137205889831 0.0011571062333850981 30-31 2.690944728802554E-5 0.0 0.0 0.002960039201682809 0.0011571062333850981 32-33 2.690944728802554E-5 0.0 0.0 0.00854374951394811 0.0012109251279611491 34-35 2.690944728802554E-5 0.0 0.0 0.017114408475184244 0.0012109251279611491 36-37 5.381889457605108E-5 1.345472364401277E-5 0.0 0.030219309304452678 0.0012378345752491748 38-39 5.381889457605108E-5 2.690944728802554E-5 0.0 0.050307211704963745 0.0012378345752491748 40-41 5.381889457605108E-5 5.381889457605108E-5 0.0 0.07139076365513175 0.0012378345752491748 42-43 5.381889457605108E-5 8.072834186407662E-5 2.690944728802554E-5 0.09238013253979166 0.0012378345752491748 44-45 5.381889457605108E-5 8.072834186407662E-5 2.690944728802554E-5 0.11978740460264568 0.0012378345752491748 46-47 5.381889457605108E-5 8.072834186407662E-5 2.690944728802554E-5 0.15302057200335722 0.0012512892988931876 48-49 5.381889457605108E-5 8.072834186407662E-5 2.690944728802554E-5 0.19011524508990044 0.0012647440225372003 50-51 5.381889457605108E-5 8.072834186407662E-5 2.690944728802554E-5 0.23282053793599694 0.0013185629171132513 52-53 6.727361822006385E-5 8.072834186407662E-5 2.690944728802554E-5 0.28802526904738135 0.001399291258977328 54-55 8.072834186407662E-5 8.072834186407662E-5 2.690944728802554E-5 0.3502802753482284 0.0014396554299093663 56-57 9.41830655080894E-5 8.072834186407662E-5 2.690944728802554E-5 0.4209713933738715 0.0015069290481294303 58-59 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.500556083728207 0.0015607479427054813 60-61 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.5861684902750603 0.0015876573899935068 62-63 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.6767456898465543 0.00174911407372166 64-65 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.7698254680158345 0.0017760235210096855 66-67 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.8736555703766812 0.001802932968297711 68-69 1.0763778915210216E-4 8.072834186407662E-5 2.690944728802554E-5 0.9878861741143494 0.0018298424155857365 70-71 1.0763778915210216E-4 8.072834186407662E-5 4.036417093203831E-5 1.1154100448123025 0.0018567518628737623 72-73 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 1.2462706869739706 0.0019643896520258643 74-75 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 1.3789208073802928 0.0019912990993138898 76-77 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 1.5245681908267308 0.0020182085466019153 78-79 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 1.6897518330042756 0.0020585727175339535 80-81 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 1.8749291945168234 0.002112391612110005 82-83 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 2.0606312902514876 0.0021527557830420432 84-85 1.0763778915210216E-4 8.072834186407662E-5 5.381889457605108E-5 2.2498585235808832 0.0021527557830420432 86-87 1.2109251279611492E-4 8.072834186407662E-5 6.727361822006385E-5 2.4507375475859936 0.002220029401262107 88-89 1.345472364401277E-4 8.072834186407662E-5 8.072834186407662E-5 2.66551530111537 0.002327667190414209 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2980 0.0 54.146507 1 GTATCAA 5720 0.0 45.575485 1 ATCAACG 7125 0.0 36.635868 3 TCAACGC 7275 0.0 36.215687 4 CAACGCA 7470 0.0 35.01704 5 TATCAAC 7360 0.0 34.79025 2 AACGCAG 8055 0.0 32.59151 6 ACGCAGA 9085 0.0 28.308296 7 GTACATG 7510 0.0 27.528383 1 CGCAGAG 9475 0.0 27.309923 8 TACATGG 7825 0.0 26.281338 2 ACATGGG 8045 0.0 24.178139 3 GCAGAGT 10370 0.0 23.16281 9 GTGGTAT 1370 0.0 22.083477 1 CATGGGG 6145 0.0 20.740856 4 CAGAGTA 9645 0.0 20.18782 10-11 AGAGTAC 9050 0.0 19.805601 10-11 GAGTACT 6575 0.0 19.70587 12-13 GAGTACA 5335 0.0 18.997585 1 TGGTATC 1690 0.0 18.789946 2 >>END_MODULE